##gff-version 3
##sequence-region BBa_I12240 1 2317
BBa_I12240	.	engineered_region	63	1329	.	+	0	ID=BBa_I12224;Name=BBa_I12224
BBa_I12240	.	terminator	1201	1280	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12224
BBa_I12240	.	stem_loop	1212	1255	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12240	.	engineered_region	1201	1280	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12240	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I12224
BBa_I12240	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I12240	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I12240	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I12240	.	CDS	82	1167	.	+	0	ID=BBa_C0013;Name=BBa_C0013;Parent=BBa_I12224
BBa_I12240	.	engineered_tag	1168	1192	.	+	0	ID=annotation2214005;Name=Help:Barcodes;Parent=BBa_C0013
BBa_I12240	.	terminator	1289	1329	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12224
BBa_I12240	.	polyA_site	1316	1329	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12240	.	stem_loop	1296	1315	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12240	.	engineered_region	1289	1329	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12240	.	stop_codon	1322	1322	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12240	.	promoter	1338	1391	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I12240	.	promoter	1357	1362	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I12240	.	operator	1340	1356	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I12240	.	operator	1363	1379	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I12240	.	promoter	1380	1385	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I12240	.	engineered_region	1338	1391	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I12240	.	engineered_region	1401	2317	.	+	0	ID=BBa_E0422;Name=BBa_E0422
BBa_I12240	.	terminator	2189	2268	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_I12240	.	stem_loop	2200	2243	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12240	.	engineered_region	2189	2268	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12240	.	terminator	2277	2317	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_I12240	.	polyA_site	2304	2317	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12240	.	stem_loop	2284	2303	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12240	.	engineered_region	2277	2317	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12240	.	stop_codon	2310	2310	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12240	.	ribosome_entry_site	1401	1412	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_I12240	.	conserved_region	1405	1408	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12240	.	engineered_region	1419	2180	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_I12240	.	stop_codon	2175	2180	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I12240	.	tag	2137	2174	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I12240	.	point_mutation	1487	1487	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I12240	.	CDS	1419	2180	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I12240	.	engineered_region	1419	2180	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I12240	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_I12240	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I12240	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I12240	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I12240	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I12240	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I12240	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I12240	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
>BBa_I12240
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtact
agatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaacca
ggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtg
gcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaa
ttgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcga
agcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccag
gatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaaca
gtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgct
gttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaatt
cagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggca
tcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcg
cgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatc
aaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggca
atcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgtt
ggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgt
agatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgt
gagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgg
tgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagtt
cagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg
cccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagc
agcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaacta
caagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggag
gacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaaga
acggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaa
cacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagacccc
aacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtaca
agaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttata