##gff-version 3 ##sequence-region BBa_I12233 1 6513 BBa_I12233 . terminator 5391 5470 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I12233 . engineered_region 5391 5470 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I12233 . stem_loop 5402 5445 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I12233 . ribosome_entry_site 63 75 . + 0 ID=BBa_B0032;Name=BBa_B0032 BBa_I12233 . ribosome_entry_site 69 72 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_I12233 . engineered_region 63 75 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_I12233 . ribosome_entry_site 63 75 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_I12233 . ribosome_entry_site 4713 4724 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I12233 . conserved_region 4717 4720 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I12233 . terminator 2334 2413 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I12233 . engineered_region 2334 2413 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I12233 . stem_loop 2345 2388 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I12233 . terminator 3358 3437 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I12233 . engineered_region 3358 3437 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I12233 . stem_loop 3369 3412 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I12233 . engineered_region 5615 6376 . + 0 ID=BBa_E0022;Name=BBa_E0022 BBa_I12233 . CDS 5615 6376 . + 0 ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022 BBa_I12233 . point_mutation 5683 5683 . + 0 ID=annotation2155;Name=A;Parent=BBa_E0022 BBa_I12233 . stop_codon 6371 6376 . + 0 ID=annotation2154;Name=2;Parent=BBa_E0022 BBa_I12233 . engineered_region 5615 6376 . + 0 ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022 BBa_I12233 . tag 6333 6370 . + 0 ID=annotation2153;Name=SsrA;Parent=BBa_E0022 BBa_I12233 . terminator 1289 1329 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I12233 . polyA_site 1316 1329 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I12233 . engineered_region 1289 1329 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I12233 . stop_codon 1322 1322 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I12233 . stem_loop 1296 1315 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I12233 . terminator 6385 6464 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I12233 . engineered_region 6385 6464 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I12233 . stem_loop 6396 6439 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I12233 . promoter 1 54 . + 0 ID=BBa_R0053;Name=BBa_R0053 BBa_I12233 . promoter 18 23 . + 0 ID=annotation2038;Name=-35;Parent=BBa_R0053 BBa_I12233 . non_covalent_binding_site 11 28 . + 0 ID=annotation2036;Name=OR2;Parent=BBa_R0053 BBa_I12233 . non_covalent_binding_site 1 3 . + 0 ID=annotation2035;Name=OR3;Parent=BBa_R0053 BBa_I12233 . engineered_region 1 54 . + 0 ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053 BBa_I12233 . promoter 30 35 . + 0 ID=annotation2042;Name=-10;Parent=BBa_R0053 BBa_I12233 . non_covalent_binding_site 34 51 . + 0 ID=annotation2037;Name=OR1;Parent=BBa_R0053 BBa_I12233 . promoter 8 13 . + 0 ID=annotation2041;Name=-35;Parent=BBa_R0053 BBa_I12233 . ribosome_entry_site 5597 5608 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_I12233 . conserved_region 5601 5604 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I12233 . terminator 2422 2462 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I12233 . polyA_site 2449 2462 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I12233 . engineered_region 2422 2462 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I12233 . stop_codon 2455 2455 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I12233 . stem_loop 2429 2448 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I12233 . promoter 2471 2670 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_I12233 . CDS 2471 2558 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_I12233 . start_codon 2643 2643 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_I12233 . engineered_region 2471 2670 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_I12233 . non_covalent_binding_site 2559 2596 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_I12233 . promoter 2631 2636 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_I12233 . non_covalent_binding_site 2636 2670 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_I12233 . promoter 2607 2612 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_I12233 . promoter 5528 5588 . + 0 ID=BBa_I12212;Name=BBa_I12212 BBa_I12233 . promoter 5553 5557 . + 0 ID=annotation1031340;Name=-10;Parent=BBa_I12212 BBa_I12233 . promoter 5528 5533 . + 0 ID=annotation1031337;Name=-35;Parent=BBa_I12212 BBa_I12233 . non_covalent_binding_site 5559 5577 . + 0 ID=annotation1031341;Name=O1;Parent=BBa_I12212 BBa_I12233 . non_covalent_binding_site 5564 5564 . + 0 ID=annotation1057574;Name=O1 mutation;Parent=BBa_I12212 BBa_I12233 . non_covalent_binding_site 5529 5547 . + 0 ID=annotation1031338;Name=TetR O2;Parent=BBa_I12212 BBa_I12233 . non_covalent_binding_site 5534 5534 . + 0 ID=annotation1057573;Name=O2 mutation;Parent=BBa_I12212 BBa_I12233 . promoter 5549 5554 . + 0 ID=annotation1031339;Name=-35;Parent=BBa_I12212 BBa_I12233 . promoter 5572 5577 . + 0 ID=annotation1031342;Name=-10;Parent=BBa_I12212 BBa_I12233 . promoter 1338 1391 . + 0 ID=BBa_R0053;Name=BBa_R0053 BBa_I12233 . promoter 1355 1360 . + 0 ID=annotation2038;Name=-35;Parent=BBa_R0053 BBa_I12233 . non_covalent_binding_site 1348 1365 . + 0 ID=annotation2036;Name=OR2;Parent=BBa_R0053 BBa_I12233 . non_covalent_binding_site 1338 1340 . + 0 ID=annotation2035;Name=OR3;Parent=BBa_R0053 BBa_I12233 . engineered_region 1338 1391 . + 0 ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053 BBa_I12233 . promoter 1367 1372 . + 0 ID=annotation2042;Name=-10;Parent=BBa_R0053 BBa_I12233 . non_covalent_binding_site 1371 1388 . + 0 ID=annotation2037;Name=OR1;Parent=BBa_R0053 BBa_I12233 . promoter 1345 1350 . + 0 ID=annotation2041;Name=-35;Parent=BBa_R0053 BBa_I12233 . terminator 1201 1280 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_I12233 . engineered_region 1201 1280 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I12233 . stem_loop 1212 1255 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I12233 . CDS 1419 2300 . + 0 ID=BBa_I12205;Name=BBa_I12205 BBa_I12233 . CDS 1419 2300 . + 0 ID=annotation1032104;Name=araC;Parent=BBa_I12205 BBa_I12233 . engineered_tag 2301 2325 . + 0 ID=annotation2214023;Name=Help:Barcodes;Parent=BBa_I12205 BBa_I12233 . promoter 3495 4704 . + 0 ID=BBa_I0500;Name=BBa_I0500 BBa_I12233 . sequence_feature 3495 4573 . + 0 ID=annotation1498843;Name=AraC;Parent=BBa_I0500 BBa_I12233 . promoter 4574 4704 . + 0 ID=annotation1498841;Name=Pbad;Parent=BBa_I0500 BBa_I12233 . promoter 4537 4608 . + 0 ID=annotation1498842;Name=PC;Parent=BBa_I0500 BBa_I12233 . terminator 5479 5519 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I12233 . polyA_site 5506 5519 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I12233 . engineered_region 5479 5519 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I12233 . stop_codon 5512 5512 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I12233 . stem_loop 5486 5505 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I12233 . ribosome_entry_site 1400 1412 . + 0 ID=BBa_B0032;Name=BBa_B0032 BBa_I12233 . ribosome_entry_site 1406 1409 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_I12233 . engineered_region 1400 1412 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_I12233 . ribosome_entry_site 1400 1412 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_I12233 . CDS 82 1167 . + 0 ID=BBa_C0013;Name=BBa_C0013 BBa_I12233 . engineered_tag 1168 1192 . + 0 ID=annotation2214005;Name=Help:Barcodes;Parent=BBa_C0013 BBa_I12233 . terminator 3446 3486 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I12233 . polyA_site 3473 3486 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I12233 . engineered_region 3446 3486 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I12233 . stop_codon 3479 3479 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I12233 . stem_loop 3453 3472 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I12233 . CDS 2698 3324 . + 0 ID=BBa_I12202;Name=BBa_I12202 BBa_I12233 . CDS 2698 3324 . + 0 ID=annotation1031374;Name=tetR;Parent=BBa_I12202 BBa_I12233 . engineered_tag 3325 3349 . + 0 ID=annotation2214021;Name=Help:Barcodes;Parent=BBa_I12202 BBa_I12233 . CDS 4731 5357 . + 0 ID=BBa_I12203;Name=BBa_I12203 BBa_I12233 . CDS 4731 5357 . + 0 ID=annotation1031381;Name=tetR-4C;Parent=BBa_I12203 BBa_I12233 . engineered_tag 5358 5382 . + 0 ID=annotation2214022;Name=Help:Barcodes;Parent=BBa_I12203 BBa_I12233 . terminator 6473 6513 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_I12233 . polyA_site 6500 6513 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I12233 . engineered_region 6473 6513 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I12233 . stop_codon 6506 6506 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I12233 . stem_loop 6480 6499 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I12233 . ribosome_entry_site 2679 2691 . + 0 ID=BBa_B0032;Name=BBa_B0032 BBa_I12233 . ribosome_entry_site 2685 2688 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_I12233 . engineered_region 2679 2691 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_I12233 . ribosome_entry_site 2679 2691 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 >BBa_I12233 aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtact agatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaacca ggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtg gcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaa ttgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcga agcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccag gatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaaca gtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgct gttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaatt cagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggca tcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcg cgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatc aaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggca atcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgtt ggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgt agatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaa cttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatgg ctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggc caacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcag ggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatc actacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatg gcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctg tttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgt tactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacat cagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtca catcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttt tgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatt taaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttg tcataataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct ttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacga caggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggct ttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacag gaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcga aggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgg gctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggc aagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttagg tacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagag aatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgcta aagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaagg tgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtct taataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc tgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctc gggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtgg cgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaat ccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaa ttgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgt taatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttg cccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattg gcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgc gagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattc tcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcg ataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggca gcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcat cagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctg taacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgatt atttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatc gcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagat tagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaact cgcccagaagctaggtgtagcgcagcaaacattgatgtggcatgtaaaaaataagcgggctttgctcgacgccttagcc attgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacg ctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaaca gtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagc gctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacaccta ctactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttatt cggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaataacactgatagtgctag tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttc tctgtcactgatagggagtggtaaaataactctgtcaatgatagagtggattcaaaaatactagagaaagaggagaaat actagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacgg ccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccacc ggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgacc acatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacga cggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgac ttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgaca agcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccacta ccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagc aaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacg agctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataa aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac tggctcaccttcgggtgggcctttctgcgtttata