##gff-version 3
##sequence-region BBa_I12232 1 5307
BBa_I12232	.	promoter	3350	3498	.	+	0	ID=BBa_R0080;Name=BBa_R0080
BBa_I12232	.	non_covalent_binding_site	3392	3421	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_I12232	.	promoter	3462	3467	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_I12232	.	promoter	3485	3490	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_I12232	.	non_covalent_binding_site	3353	3378	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_I12232	.	operator	3355	3393	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_I12232	.	non_covalent_binding_site	3422	3450	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_I12232	.	engineered_region	1400	2462	.	+	0	ID=BBa_I12225;Name=BBa_I12225
BBa_I12232	.	ribosome_entry_site	1400	1412	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I12225
BBa_I12232	.	ribosome_entry_site	1406	1409	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I12232	.	engineered_region	1400	1412	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I12232	.	ribosome_entry_site	1400	1412	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I12232	.	CDS	1419	2300	.	+	0	ID=BBa_I12205;Name=BBa_I12205;Parent=BBa_I12225
BBa_I12232	.	engineered_tag	2301	2325	.	+	0	ID=annotation2214023;Name=Help:Barcodes;Parent=BBa_I12205
BBa_I12232	.	CDS	1419	2300	.	+	0	ID=annotation1032104;Name=araC;Parent=BBa_I12205
BBa_I12232	.	terminator	2422	2462	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12225
BBa_I12232	.	stop_codon	2455	2455	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12232	.	polyA_site	2449	2462	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12232	.	stem_loop	2429	2448	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12232	.	engineered_region	2422	2462	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12232	.	terminator	2334	2413	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12225
BBa_I12232	.	stem_loop	2345	2388	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12232	.	engineered_region	2334	2413	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12232	.	promoter	4322	4382	.	+	0	ID=BBa_I12212;Name=BBa_I12212
BBa_I12232	.	non_covalent_binding_site	4358	4358	.	+	0	ID=annotation1057574;Name=O1 mutation;Parent=BBa_I12212
BBa_I12232	.	promoter	4366	4371	.	+	0	ID=annotation1031342;Name=-10;Parent=BBa_I12212
BBa_I12232	.	non_covalent_binding_site	4353	4371	.	+	0	ID=annotation1031341;Name=O1;Parent=BBa_I12212
BBa_I12232	.	promoter	4347	4351	.	+	0	ID=annotation1031340;Name=-10;Parent=BBa_I12212
BBa_I12232	.	non_covalent_binding_site	4328	4328	.	+	0	ID=annotation1057573;Name=O2 mutation;Parent=BBa_I12212
BBa_I12232	.	promoter	4343	4348	.	+	0	ID=annotation1031339;Name=-35;Parent=BBa_I12212
BBa_I12232	.	promoter	4322	4327	.	+	0	ID=annotation1031337;Name=-35;Parent=BBa_I12212
BBa_I12232	.	non_covalent_binding_site	4323	4341	.	+	0	ID=annotation1031338;Name=TetR O2;Parent=BBa_I12212
BBa_I12232	.	engineered_region	2534	3341	.	+	0	ID=BBa_I12222;Name=BBa_I12222
BBa_I12232	.	terminator	3301	3341	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12222
BBa_I12232	.	stop_codon	3334	3334	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12232	.	polyA_site	3328	3341	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12232	.	stem_loop	3308	3327	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12232	.	engineered_region	3301	3341	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12232	.	CDS	2553	3179	.	+	0	ID=BBa_I12202;Name=BBa_I12202;Parent=BBa_I12222
BBa_I12232	.	CDS	2553	3179	.	+	0	ID=annotation1031374;Name=tetR;Parent=BBa_I12202
BBa_I12232	.	engineered_tag	3180	3204	.	+	0	ID=annotation2214021;Name=Help:Barcodes;Parent=BBa_I12202
BBa_I12232	.	ribosome_entry_site	2534	2546	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I12222
BBa_I12232	.	ribosome_entry_site	2540	2543	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I12232	.	engineered_region	2534	2546	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I12232	.	ribosome_entry_site	2534	2546	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I12232	.	terminator	3213	3292	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12222
BBa_I12232	.	stem_loop	3224	3267	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12232	.	engineered_region	3213	3292	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12232	.	engineered_region	63	1329	.	+	0	ID=BBa_I12224;Name=BBa_I12224
BBa_I12232	.	terminator	1201	1280	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12224
BBa_I12232	.	stem_loop	1212	1255	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12232	.	engineered_region	1201	1280	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12232	.	CDS	82	1167	.	+	0	ID=BBa_C0013;Name=BBa_C0013;Parent=BBa_I12224
BBa_I12232	.	engineered_tag	1168	1192	.	+	0	ID=annotation2214005;Name=Help:Barcodes;Parent=BBa_C0013
BBa_I12232	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I12224
BBa_I12232	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I12232	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I12232	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I12232	.	terminator	1289	1329	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12224
BBa_I12232	.	stop_codon	1322	1322	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12232	.	polyA_site	1316	1329	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12232	.	stem_loop	1296	1315	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12232	.	engineered_region	1289	1329	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12232	.	engineered_region	4391	5307	.	+	0	ID=BBa_E0422;Name=BBa_E0422
BBa_I12232	.	engineered_region	4409	5170	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_I12232	.	tag	5127	5164	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I12232	.	CDS	4409	5170	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I12232	.	engineered_region	4409	5170	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_I12232	.	stop_codon	5165	5170	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I12232	.	point_mutation	4477	4477	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I12232	.	terminator	5267	5307	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_I12232	.	stop_codon	5300	5300	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12232	.	polyA_site	5294	5307	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12232	.	stem_loop	5274	5293	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12232	.	engineered_region	5267	5307	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12232	.	terminator	5179	5258	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_I12232	.	stem_loop	5190	5233	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12232	.	engineered_region	5179	5258	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12232	.	ribosome_entry_site	4391	4402	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_I12232	.	conserved_region	4395	4398	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12232	.	promoter	1338	1391	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_I12232	.	non_covalent_binding_site	1338	1340	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I12232	.	engineered_region	1338	1391	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I12232	.	promoter	1367	1372	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I12232	.	non_covalent_binding_site	1348	1365	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I12232	.	promoter	1345	1350	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I12232	.	non_covalent_binding_site	1371	1388	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I12232	.	promoter	1355	1360	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I12232	.	engineered_region	3507	4313	.	+	0	ID=BBa_I12223;Name=BBa_I12223
BBa_I12232	.	ribosome_entry_site	3507	3518	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12223
BBa_I12232	.	conserved_region	3511	3514	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12232	.	CDS	3525	4151	.	+	0	ID=BBa_I12203;Name=BBa_I12203;Parent=BBa_I12223
BBa_I12232	.	CDS	3525	4151	.	+	0	ID=annotation1031381;Name=tetR-4C;Parent=BBa_I12203
BBa_I12232	.	engineered_tag	4152	4176	.	+	0	ID=annotation2214022;Name=Help:Barcodes;Parent=BBa_I12203
BBa_I12232	.	terminator	4185	4264	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12223
BBa_I12232	.	stem_loop	4196	4239	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12232	.	engineered_region	4185	4264	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12232	.	terminator	4273	4313	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12223
BBa_I12232	.	stop_codon	4306	4306	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12232	.	polyA_site	4300	4313	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12232	.	stem_loop	4280	4299	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12232	.	engineered_region	4273	4313	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12232	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_I12232	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I12232	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I12232	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I12232	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I12232	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I12232	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I12232	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I12232	.	promoter	2471	2524	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I12232	.	promoter	2513	2518	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I12232	.	operator	2473	2489	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I12232	.	promoter	2490	2495	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I12232	.	engineered_region	2471	2524	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I12232	.	operator	2496	2512	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
>BBa_I12232
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtact
agatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaacca
ggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtg
gcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaa
ttgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcga
agcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccag
gatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaaca
gtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgct
gttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaatt
cagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggca
tcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcg
cgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatc
aaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggca
atcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgtt
ggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataacactgatagtgctagtgt
agatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaa
cttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaagtactagatgg
ctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggc
caacggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcag
ggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatc
actacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatg
gcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctg
tttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgt
tactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacat
cagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcttgtcgccgtcgcgtctgtca
catcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcattagtcaggcgaagctgcttt
tgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatt
taaaaaatgcaccggggccagcccgagcgagtttcgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttg
tcataataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatac
tagagtcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatg
aggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgt
aaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaa
ggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaa
aagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaagg
tttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagag
catcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattat
ttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatg
tgaaagtgggtcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagaggcgtaacaaaagtgtctataatcacggcagaaaagtccacattgatta
tttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcg
caactctctactgtttctccattactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgatta
acagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagc
gcagcaaacattgatgtggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcac
catactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctt
tactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatca
attagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttacttta
ggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccat
tattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatg
cggattagaaaaacaacttaaatgtgaaagtgggtcttaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttctctgtcactgatagggagtgg
taaaataactctgtcaatgatagagtggattcaaaaatactagagaaagaggagaaatactagatggtgagcaagggcg
aggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccgg
cgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctgg
cccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttct
tcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgc
cgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatc
ctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaagg
ccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcgg
cgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgc
gatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctg
caaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttata