##gff-version 3
##sequence-region BBa_I12220 1 1018
BBa_I12220	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I12220	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I12220	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I12220	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I12220	.	CDS	20	856	.	+	0	ID=BBa_I12200;Name=BBa_I12200
BBa_I12220	.	engineered_tag	857	881	.	+	0	ID=annotation2214019;Name=Help:Barcodes;Parent=BBa_I12200
BBa_I12220	.	CDS	731	850	.	+	0	ID=annotation1031161;Name=gal4 dimerization domain;Parent=BBa_I12200
BBa_I12220	.	CDS	20	730	.	+	0	ID=annotation1031160;Name=cI lambda;Parent=BBa_I12200
BBa_I12220	.	stem_loop	851	856	.	+	0	ID=annotation1031174;Name=Terminator;Parent=BBa_I12200
BBa_I12220	.	CDS	20	853	.	+	0	ID=annotation1031472;Name=cI lambda;Parent=BBa_I12200
BBa_I12220	.	terminator	978	1018	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I12220	.	polyA_site	1005	1018	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12220	.	engineered_region	978	1018	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12220	.	stem_loop	985	1004	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12220	.	stop_codon	1011	1011	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12220	.	terminator	890	969	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I12220	.	stem_loop	901	944	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12220	.	engineered_region	890	969	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I12220
tcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaa
gcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcag
gcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttag
cgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttaga
agtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtg
atgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgac
cgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggt
gatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttac
aaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtg
gcctgaagagacgtttggcgaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagacctt
gacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattattttaataaccctgatagtgct
agtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata