##gff-version 3
##sequence-region BBa_I12058 1 3925
BBa_I12058	.	engineered_region	3009	3925	.	+	0	ID=BBa_E0434;Name=BBa_E0434
BBa_I12058	.	engineered_region	3027	3788	.	+	0	ID=BBa_E0034;Name=BBa_E0034;Parent=BBa_E0434
BBa_I12058	.	tag	3744	3782	.	+	0	ID=annotation1245672;Name=AAV;Parent=BBa_E0034
BBa_I12058	.	stop_codon	3783	3788	.	+	0	ID=annotation1245671;Name=stop;Parent=BBa_E0034
BBa_I12058	.	ribosome_entry_site	3009	3020	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0434
BBa_I12058	.	conserved_region	3013	3016	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12058	.	terminator	3885	3925	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0434
BBa_I12058	.	stop_codon	3918	3918	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12058	.	polyA_site	3912	3925	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12058	.	stem_loop	3892	3911	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12058	.	engineered_region	3885	3925	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12058	.	terminator	3797	3876	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0434
BBa_I12058	.	stem_loop	3808	3851	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12058	.	engineered_region	3797	3876	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12058	.	engineered_region	1002	1882	.	+	0	ID=BBa_I12054;Name=BBa_I12054
BBa_I12058	.	CDS	1110	1745	.	+	0	ID=BBa_C0056;Name=BBa_C0056;Parent=BBa_I12054
BBa_I12058	.	CDS	1110	1745	.	+	0	ID=annotation1063931;Name=cI 434;Parent=BBa_C0056
BBa_I12058	.	stop_codon	1740	1745	.	+	0	ID=annotation1063930;Name=2;Parent=BBa_C0056
BBa_I12058	.	terminator	1842	1882	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12054
BBa_I12058	.	stop_codon	1875	1875	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12058	.	polyA_site	1869	1882	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12058	.	stem_loop	1849	1868	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12058	.	engineered_region	1842	1882	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12058	.	ribosome_entry_site	1092	1103	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12054
BBa_I12058	.	conserved_region	1096	1099	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12058	.	promoter	1002	1083	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_I12054
BBa_I12058	.	promoter	1072	1077	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_I12058	.	non_covalent_binding_site	1010	1026	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_I12058	.	sequence_alteration	1060	1066	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_I12058	.	promoter	1049	1054	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_I12058	.	non_covalent_binding_site	1034	1050	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_I12058	.	terminator	1754	1833	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12054
BBa_I12058	.	stem_loop	1765	1808	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12058	.	engineered_region	1754	1833	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12058	.	engineered_region	1	993	.	+	0	ID=BBa_I12024;Name=BBa_I12024
BBa_I12058	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_I12024
BBa_I12058	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I12058	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I12058	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I12058	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I12058	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I12058	.	CDS	82	856	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I12024
BBa_I12058	.	engineered_tag	832	856	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I12058	.	tag	793	825	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12058	.	CDS	85	792	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12058	.	terminator	865	993	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I12024
BBa_I12058	.	terminator	865	944	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I12058	.	stem_loop	876	919	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12058	.	engineered_region	865	944	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12058	.	terminator	953	993	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I12058	.	stop_codon	986	986	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12058	.	polyA_site	980	993	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12058	.	stem_loop	960	979	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12058	.	engineered_region	953	993	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12058	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12024
BBa_I12058	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12058	.	engineered_region	1891	3000	.	+	0	ID=BBa_I12055;Name=BBa_I12055
BBa_I12058	.	promoter	1891	1972	.	+	0	ID=BBa_I12006;Name=BBa_I12006;Parent=BBa_I12055
BBa_I12058	.	non_covalent_binding_site	1899	1915	.	+	0	ID=annotation786500;Name=OR1 lambda;Parent=BBa_I12006
BBa_I12058	.	non_covalent_binding_site	1923	1939	.	+	0	ID=annotation786518;Name=OR2 lambda;Parent=BBa_I12006
BBa_I12058	.	promoter	1938	1943	.	+	0	ID=annotation837284;Name=-35;Parent=BBa_I12006
BBa_I12058	.	non_covalent_binding_site	1946	1959	.	+	0	ID=annotation786365;Name=OR1 434;Parent=BBa_I12006
BBa_I12058	.	promoter	1961	1966	.	+	0	ID=annotation837228;Name=-10;Parent=BBa_I12006
BBa_I12058	.	terminator	2870	2910	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12055
BBa_I12058	.	stop_codon	2903	2903	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12058	.	polyA_site	2897	2910	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12058	.	stem_loop	2877	2896	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12058	.	engineered_region	2870	2910	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12058	.	promoter	2919	3000	.	+	0	ID=BBa_I12006;Name=BBa_I12006;Parent=BBa_I12055
BBa_I12058	.	non_covalent_binding_site	2927	2943	.	+	0	ID=annotation786500;Name=OR1 lambda;Parent=BBa_I12006
BBa_I12058	.	non_covalent_binding_site	2951	2967	.	+	0	ID=annotation786518;Name=OR2 lambda;Parent=BBa_I12006
BBa_I12058	.	promoter	2966	2971	.	+	0	ID=annotation837284;Name=-35;Parent=BBa_I12006
BBa_I12058	.	non_covalent_binding_site	2974	2987	.	+	0	ID=annotation786365;Name=OR1 434;Parent=BBa_I12006
BBa_I12058	.	promoter	2989	2994	.	+	0	ID=annotation837228;Name=-10;Parent=BBa_I12006
BBa_I12058	.	terminator	2782	2861	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12055
BBa_I12058	.	stem_loop	2793	2836	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12058	.	engineered_region	2782	2861	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12058	.	CDS	1999	2748	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I12055
BBa_I12058	.	engineered_tag	2749	2773	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I12058	.	tag	2710	2742	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12058	.	CDS	2002	2709	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12058	.	ribosome_entry_site	1981	1992	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12055
BBa_I12058	.	conserved_region	1985	1988	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I12058
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaa
gaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattt
aatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcc
cttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccc
tgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggta
agcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcca
agccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccag
acttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacag
tacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttg
gcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaaccattatcaccgccagaggtaa
aatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagagaaagaggagaaatac
tagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtgg
ggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagc
tcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaa
gggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattg
atgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacc
tgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgta
gccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctga
atccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgt
ataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaacc
attatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttac
tagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgcct
taaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcag
tcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaag
ttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcact
tagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaa
ggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattcca
tgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagcc
aggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgttt
ttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtc
agtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtg
tagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcaac
cattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgtta
ctagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagc
tggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccct
gaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgc
ttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgca
ccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcat
cgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaac
gtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcg
tgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgag
ctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccggg
atcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctgctgctgtttaataatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata