##gff-version 3
##sequence-region BBa_I12046 1 2111
BBa_I12046	.	ribosome_entry_site	1038	1049	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I12046	.	conserved_region	1042	1045	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12046	.	engineered_region	1058	1974	.	+	0	ID=BBa_E0432;Name=BBa_E0432
BBa_I12046	.	ribosome_entry_site	1058	1069	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_I12046	.	conserved_region	1062	1065	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12046	.	terminator	1846	1925	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_I12046	.	engineered_region	1846	1925	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12046	.	stem_loop	1857	1900	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12046	.	terminator	1934	1974	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_I12046	.	polyA_site	1961	1974	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12046	.	engineered_region	1934	1974	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12046	.	stem_loop	1941	1960	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12046	.	stop_codon	1967	1967	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12046	.	engineered_region	1076	1837	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_I12046	.	tag	1793	1831	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I12046	.	sequence_alteration	1317	1317	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I12046	.	CDS	1076	1837	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I12046	.	stop_codon	1832	1837	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I12046	.	engineered_region	1076	1837	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I12046	.	ribosome_entry_site	237	248	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I12046	.	conserved_region	241	244	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12046	.	promoter	138	228	.	+	0	ID=BBa_I12040;Name=BBa_I12040
BBa_I12046	.	non_covalent_binding_site	193	206	.	+	0	ID=annotation1059301;Name=OR1 434;Parent=BBa_I12040
BBa_I12046	.	non_covalent_binding_site	170	186	.	+	0	ID=annotation1059299;Name=OR2 lambda;Parent=BBa_I12040
BBa_I12046	.	promoter	185	190	.	+	0	ID=annotation1059300;Name=-35;Parent=BBa_I12040
BBa_I12046	.	start_codon	220	220	.	+	0	ID=annotation1062263;Name=start;Parent=BBa_I12040
BBa_I12046	.	non_covalent_binding_site	146	162	.	+	0	ID=annotation1059298;Name=OR1 lambda;Parent=BBa_I12040
BBa_I12046	.	promoter	208	213	.	+	0	ID=annotation1059302;Name=-10;Parent=BBa_I12040
BBa_I12046	.	non_covalent_binding_site	215	228	.	+	0	ID=annotation1059303;Name=OR2 434;Parent=BBa_I12040
BBa_I12046	.	terminator	1	129	.	+	0	ID=BBa_B0025;Name=BBa_B0025
BBa_I12046	.	engineered_region	1	41	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_I12046	.	engineered_region	50	129	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
BBa_I12046	.	terminator	1983	2111	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I12046	.	terminator	2071	2111	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I12046	.	polyA_site	2098	2111	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12046	.	engineered_region	2071	2111	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12046	.	stem_loop	2078	2097	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12046	.	stop_codon	2104	2104	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12046	.	terminator	1983	2062	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I12046	.	engineered_region	1983	2062	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12046	.	stem_loop	1994	2037	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12046	.	CDS	255	1029	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I12046	.	tag	966	998	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12046	.	CDS	258	965	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12046	.	engineered_tag	1005	1029	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
>BBa_I12046
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggcaaccattatcaccgccaga
ggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtagatacttacaatgtatcttgttactagaga
aagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagc
aatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggc
gttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcg
ttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaag
tgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgat
gcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccg
caccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtga
tttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaa
ccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggc
ctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatc
gctactagagaaagaggagaaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccg
gggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcga
tgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgacc
accttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgc
ccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcga
gggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctg
gagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatcc
gccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgct
gctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctg
ctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaact
acgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata