##gff-version 3
##sequence-region BBa_I12045 1 4137
BBa_I12045	.	promoter	1001	3111	.	+	0	ID=BBa_I12044;Name=BBa_I12044
BBa_I12045	.	promoter	1138	1228	.	+	0	ID=BBa_I12036;Name=BBa_I12036;Parent=BBa_I12044
BBa_I12045	.	non_covalent_binding_site	1170	1186	.	+	0	ID=annotation1056736;Name=OR2 lambda;Parent=BBa_I12036
BBa_I12045	.	start_codon	1220	1220	.	+	0	ID=annotation1062203;Name=start;Parent=BBa_I12036
BBa_I12045	.	promoter	1185	1190	.	+	0	ID=annotation1056739;Name=-35;Parent=BBa_I12036
BBa_I12045	.	non_covalent_binding_site	1193	1206	.	+	0	ID=annotation1056737;Name=OR1 434;Parent=BBa_I12036
BBa_I12045	.	promoter	1208	1213	.	+	0	ID=annotation1056740;Name=-10;Parent=BBa_I12036
BBa_I12045	.	non_covalent_binding_site	1215	1228	.	+	0	ID=annotation1056738;Name=OR2 434;Parent=BBa_I12036
BBa_I12045	.	non_covalent_binding_site	1146	1162	.	+	0	ID=annotation1056735;Name=OR1 lambda;Parent=BBa_I12036
BBa_I12045	.	terminator	2983	3111	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I12044
BBa_I12045	.	terminator	2983	3062	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I12045	.	engineered_region	2983	3062	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12045	.	stem_loop	2994	3037	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12045	.	terminator	3071	3111	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I12045	.	engineered_region	3071	3111	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12045	.	stem_loop	3078	3097	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12045	.	polyA_site	3098	3111	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12045	.	stop_codon	3104	3104	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12045	.	ribosome_entry_site	1237	1248	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12044
BBa_I12045	.	conserved_region	1241	1244	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12045	.	CDS	1255	2029	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I12044
BBa_I12045	.	engineered_tag	2005	2029	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I12045	.	CDS	1258	1965	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12045	.	tag	1966	1998	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12045	.	terminator	1001	1129	.	+	0	ID=BBa_B0025;Name=BBa_B0025;Parent=BBa_I12044
BBa_I12045	.	engineered_region	1050	1129	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
BBa_I12045	.	engineered_region	1001	1041	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_I12045	.	ribosome_entry_site	2038	2049	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12044
BBa_I12045	.	conserved_region	2042	2045	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12045	.	engineered_region	2058	2974	.	+	0	ID=BBa_E0432;Name=BBa_E0432;Parent=BBa_I12044
BBa_I12045	.	terminator	2934	2974	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_I12045	.	engineered_region	2934	2974	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12045	.	stem_loop	2941	2960	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12045	.	polyA_site	2961	2974	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12045	.	stop_codon	2967	2967	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12045	.	ribosome_entry_site	2058	2069	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_I12045	.	conserved_region	2062	2065	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12045	.	engineered_region	2076	2837	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_I12045	.	tag	2793	2831	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I12045	.	stop_codon	2832	2837	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I12045	.	engineered_region	2076	2837	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I12045	.	CDS	2076	2837	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I12045	.	sequence_alteration	2317	2317	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I12045	.	terminator	2846	2925	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_I12045	.	engineered_region	2846	2925	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12045	.	stem_loop	2857	2900	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12045	.	engineered_region	1	992	.	+	0	ID=BBa_I12038;Name=BBa_I12038
BBa_I12045	.	terminator	952	992	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12038
BBa_I12045	.	engineered_region	952	992	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12045	.	stem_loop	959	978	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12045	.	polyA_site	979	992	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12045	.	stop_codon	985	985	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12045	.	terminator	864	943	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12038
BBa_I12045	.	engineered_region	864	943	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12045	.	stem_loop	875	918	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12045	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12038
BBa_I12045	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12045	.	CDS	81	830	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I12038
BBa_I12045	.	engineered_tag	831	855	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I12045	.	CDS	84	791	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12045	.	tag	792	824	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12045	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I12038
BBa_I12045	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I12045	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I12045	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I12045	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I12045	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I12045	.	engineered_region	3120	4137	.	+	0	ID=BBa_I12002;Name=BBa_I12002
BBa_I12045	.	promoter	3257	3338	.	+	0	ID=BBa_I12007;Name=BBa_I12007;Parent=BBa_I12002
BBa_I12045	.	non_covalent_binding_site	3289	3305	.	+	0	ID=annotation937702;Name=OR2;Parent=BBa_I12007
BBa_I12045	.	promoter	3327	3332	.	+	0	ID=annotation937705;Name=-10;Parent=BBa_I12007
BBa_I12045	.	promoter	3304	3309	.	+	0	ID=annotation937703;Name=-35;Parent=BBa_I12007
BBa_I12045	.	non_covalent_binding_site	3265	3281	.	+	0	ID=annotation937701;Name=OR1;Parent=BBa_I12007
BBa_I12045	.	sequence_alteration	3315	3321	.	+	0	ID=annotation937704;Name=mutate to obliterate OR3;Parent=BBa_I12007
BBa_I12045	.	promoter	3365	4000	.	+	0	ID=BBa_I12014;Name=BBa_I12014;Parent=BBa_I12002
BBa_I12045	.	CDS	3365	4033	.	+	0	ID=annotation912810;Name=cI 434;Parent=BBa_I12014
BBa_I12045	.	terminator	3120	3248	.	+	0	ID=BBa_B0025;Name=BBa_B0025;Parent=BBa_I12002
BBa_I12045	.	engineered_region	3169	3248	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
BBa_I12045	.	engineered_region	3120	3160	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_I12045	.	ribosome_entry_site	3347	3358	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12002
BBa_I12045	.	conserved_region	3351	3354	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12045	.	terminator	4009	4137	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I12002
BBa_I12045	.	terminator	4009	4088	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I12045	.	engineered_region	4009	4088	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12045	.	stem_loop	4020	4063	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12045	.	terminator	4097	4137	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I12045	.	engineered_region	4097	4137	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12045	.	stem_loop	4104	4123	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12045	.	polyA_site	4124	4137	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12045	.	stop_codon	4130	4130	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_I12045
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaag
aaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattta
atggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccc
ttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccct
gttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaa
gcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaa
gccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccaga
cttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagt
acccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgg
cgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtataaacgcagaaaggcccacccgaag
gtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagc
ctttcgttttatttgatgcctggtactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgtt
agatattacaaactttcttgtatagatttacaatgtatcttgttactagagaaagaggagaaatactagatgagcacaa
aaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaact
tggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaat
gcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgcca
gagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctca
tgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaa
aaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttc
ctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtga
tgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatc
ccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacg
acgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatacta
gagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctgg
acggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaa
gttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttc
gcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcacca
tcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcga
gctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtc
tatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgc
agctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcta
ccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatc
actctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtga
ctctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttattt
gatgcctggtactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaat
agtggtgatagatttaacgttactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaaga
atccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacg
gtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcac
ctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggt
tcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggca
atggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgtt
agtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaag
aaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaact
gcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtataataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata