##gff-version 3
##sequence-region BBa_I12043 1 2102
BBa_I12043	.	CDS	246	1020	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I12043	.	tag	957	989	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12043	.	CDS	249	956	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12043	.	engineered_tag	996	1020	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I12043	.	terminator	1974	2102	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I12043	.	terminator	2062	2102	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I12043	.	polyA_site	2089	2102	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12043	.	engineered_region	2062	2102	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12043	.	stop_codon	2095	2095	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12043	.	stem_loop	2069	2088	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12043	.	terminator	1974	2053	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I12043	.	engineered_region	1974	2053	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12043	.	stem_loop	1985	2028	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12043	.	promoter	138	219	.	+	0	ID=BBa_I12006;Name=BBa_I12006
BBa_I12043	.	non_covalent_binding_site	146	162	.	+	0	ID=annotation786500;Name=OR1 lambda;Parent=BBa_I12006
BBa_I12043	.	non_covalent_binding_site	170	186	.	+	0	ID=annotation786518;Name=OR2 lambda;Parent=BBa_I12006
BBa_I12043	.	non_covalent_binding_site	193	206	.	+	0	ID=annotation786365;Name=OR1 434;Parent=BBa_I12006
BBa_I12043	.	promoter	208	213	.	+	0	ID=annotation837228;Name=-10;Parent=BBa_I12006
BBa_I12043	.	promoter	185	190	.	+	0	ID=annotation837284;Name=-35;Parent=BBa_I12006
BBa_I12043	.	ribosome_entry_site	1029	1040	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I12043	.	conserved_region	1033	1036	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12043	.	engineered_region	1049	1965	.	+	0	ID=BBa_E0432;Name=BBa_E0432
BBa_I12043	.	terminator	1837	1916	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_I12043	.	engineered_region	1837	1916	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12043	.	stem_loop	1848	1891	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12043	.	ribosome_entry_site	1049	1060	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_I12043	.	conserved_region	1053	1056	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12043	.	terminator	1925	1965	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_I12043	.	polyA_site	1952	1965	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12043	.	engineered_region	1925	1965	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12043	.	stop_codon	1958	1958	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12043	.	stem_loop	1932	1951	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12043	.	engineered_region	1067	1828	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_I12043	.	stop_codon	1823	1828	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I12043	.	engineered_region	1067	1828	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I12043	.	CDS	1067	1828	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I12043	.	tag	1784	1822	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I12043	.	sequence_alteration	1308	1308	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I12043	.	ribosome_entry_site	228	239	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I12043	.	conserved_region	232	235	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12043	.	terminator	1	129	.	+	0	ID=BBa_B0025;Name=BBa_B0025
BBa_I12043	.	engineered_region	1	41	.	+	0	ID=annotation369702;Name=B0012;Parent=BBa_B0025
BBa_I12043	.	engineered_region	50	129	.	+	0	ID=annotation369703;Name=B0010;Parent=BBa_B0025
>BBa_I12043
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggcaaccattatcaccgccaga
ggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagagaaagaggaga
aatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatga
aaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgct
ttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaat
ttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatga
gtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagaga
tgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacag
gctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcat
agccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaac
ccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagaga
cgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactaga
gaaagaggagaaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgc
ccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccaccta
cggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggc
tacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggct
acgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacac
cctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaac
tacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaaca
tcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccga
caaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttc
gtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttag
tagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata