##gff-version 3
##sequence-region BBa_I12020 1 5176
BBa_I12020	.	engineered_region	2299	4200	.	+	0	ID=BBa_I12023;Name=BBa_I12023
BBa_I12020	.	ribosome_entry_site	3271	3282	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12023
BBa_I12020	.	conserved_region	3275	3278	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12020	.	terminator	3160	3200	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12023
BBa_I12020	.	stem_loop	3167	3186	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12020	.	stop_codon	3193	3193	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12020	.	polyA_site	3187	3200	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12020	.	engineered_region	3160	3200	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12020	.	CDS	3289	4038	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_I12023
BBa_I12020	.	tag	4000	4032	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I12020	.	engineered_tag	4039	4063	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I12020	.	CDS	3292	3999	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I12020	.	promoter	3209	3262	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I12023
BBa_I12020	.	engineered_region	3209	3262	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I12020	.	non_covalent_binding_site	3209	3227	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I12020	.	promoter	3228	3233	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I12020	.	promoter	3251	3256	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I12020	.	non_covalent_binding_site	3234	3252	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I12020	.	terminator	4160	4200	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I12023
BBa_I12020	.	stem_loop	4167	4186	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12020	.	stop_codon	4193	4193	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12020	.	polyA_site	4187	4200	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12020	.	engineered_region	4160	4200	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12020	.	promoter	2299	2352	.	+	0	ID=BBa_R0053;Name=BBa_R0053;Parent=BBa_I12023
BBa_I12020	.	non_covalent_binding_site	2309	2326	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I12020	.	non_covalent_binding_site	2332	2349	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I12020	.	non_covalent_binding_site	2299	2301	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I12020	.	promoter	2328	2333	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I12020	.	engineered_region	2299	2352	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I12020	.	promoter	2316	2321	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I12020	.	promoter	2306	2311	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I12020	.	ribosome_entry_site	2361	2372	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12023
BBa_I12020	.	conserved_region	2365	2368	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12020	.	terminator	4072	4151	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12023
BBa_I12020	.	stem_loop	4083	4126	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12020	.	engineered_region	4072	4151	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12020	.	CDS	2379	3038	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_I12023
BBa_I12020	.	tag	2999	3032	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I12020	.	engineered_tag	3039	3063	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I12020	.	CDS	2382	2998	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I12020	.	terminator	3072	3151	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I12023
BBa_I12020	.	stem_loop	3083	3126	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12020	.	engineered_region	3072	3151	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12020	.	promoter	4209	4290	.	+	0	ID=BBa_I12006;Name=BBa_I12006
BBa_I12020	.	promoter	4279	4284	.	+	0	ID=annotation837228;Name=-10;Parent=BBa_I12006
BBa_I12020	.	non_covalent_binding_site	4241	4257	.	+	0	ID=annotation786518;Name=OR2 lambda;Parent=BBa_I12006
BBa_I12020	.	non_covalent_binding_site	4217	4233	.	+	0	ID=annotation786500;Name=OR1 lambda;Parent=BBa_I12006
BBa_I12020	.	non_covalent_binding_site	4264	4277	.	+	0	ID=annotation786365;Name=OR1 434;Parent=BBa_I12006
BBa_I12020	.	promoter	4256	4261	.	+	0	ID=annotation837284;Name=-35;Parent=BBa_I12006
BBa_I12020	.	engineered_region	4299	5176	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_I12020	.	terminator	5136	5176	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_I12020	.	stem_loop	5143	5162	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12020	.	stop_codon	5169	5169	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12020	.	polyA_site	5163	5176	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12020	.	engineered_region	5136	5176	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12020	.	ribosome_entry_site	4299	4310	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_I12020	.	conserved_region	4303	4306	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12020	.	CDS	4317	5039	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_I12020	.	terminator	5048	5127	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_I12020	.	stem_loop	5059	5102	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12020	.	engineered_region	5048	5127	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12020	.	engineered_region	1	2290	.	+	0	ID=BBa_I12022;Name=BBa_I12022
BBa_I12020	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053;Parent=BBa_I12022
BBa_I12020	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_I12020	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_I12020	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_I12020	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_I12020	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_I12020	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_I12020	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_I12020	.	engineered_region	63	1433	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121;Parent=BBa_I12022
BBa_I12020	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_I12020	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12020	.	terminator	1242	1370	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_I12020	.	terminator	1330	1370	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I12020	.	stem_loop	1337	1356	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12020	.	stop_codon	1363	1363	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12020	.	polyA_site	1357	1370	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12020	.	engineered_region	1330	1370	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12020	.	terminator	1242	1321	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I12020	.	stem_loop	1253	1296	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12020	.	engineered_region	1242	1321	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I12020	.	CDS	81	1208	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_I12020	.	CDS	81	1208	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I12020	.	engineered_tag	1209	1233	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I12020	.	engineered_region	81	1208	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I12020	.	tag	1170	1208	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I12020	.	promoter	1379	1432	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_I12020	.	promoter	1398	1403	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I12020	.	promoter	1421	1426	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I12020	.	operator	1381	1397	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I12020	.	engineered_region	1379	1432	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I12020	.	operator	1404	1420	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I12020	.	ribosome_entry_site	1442	1453	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I12022
BBa_I12020	.	conserved_region	1446	1449	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I12020	.	CDS	1460	2128	.	+	0	ID=BBa_C0052;Name=BBa_C0052;Parent=BBa_I12022
BBa_I12020	.	tag	2090	2128	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_I12020	.	CDS	1460	2128	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_I12020	.	engineered_tag	2129	2153	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_I12020	.	engineered_region	1460	2128	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_I12020	.	terminator	2162	2290	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I12022
BBa_I12020	.	terminator	2250	2290	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I12020	.	stem_loop	2257	2276	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I12020	.	stop_codon	2283	2283	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I12020	.	polyA_site	2277	2290	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I12020	.	engineered_region	2250	2290	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I12020	.	terminator	2162	2241	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I12020	.	stem_loop	2173	2216	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I12020	.	engineered_region	2162	2241	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I12020
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggagaaatacta
gatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtg
gtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccca
accgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgcc
gtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagc
ggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctgg
atgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacc
catcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaa
atcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgca
atcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaa
tgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtcc
gggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaa
ccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggt
gaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaa
actacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaaga
tactgagcacatactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagctt
ggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaacta
agcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattc
gaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgt
gaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattct
ggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatac
tggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtc
atagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagatta
tcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctg
atagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagaaagaggaga
aatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaagg
tttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggct
ttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaag
attttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtac
acggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaat
gcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaag
aagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgc
agagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgct
gcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatcccta
tcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaa
gagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctg
tcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgc
cgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtat
gaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttct
cacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcatt
ctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctc
gttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaac
tgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttc
cgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagta
gcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattaca
aactttcttgtatagatttaacgttactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcac
cggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggc
gatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtga
ccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccat
gcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttc
gagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagc
tggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagat
ccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtg
ctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcc
tgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata