##gff-version 3 ##sequence-region BBa_I12013 1 1281 BBa_I12013 . terminator 1153 1281 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_I12013 . terminator 1241 1281 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_I12013 . stem_loop 1248 1267 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I12013 . engineered_region 1241 1281 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I12013 . stop_codon 1274 1274 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I12013 . polyA_site 1268 1281 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I12013 . terminator 1153 1232 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_I12013 . engineered_region 1153 1232 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I12013 . stem_loop 1164 1207 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I12013 . engineered_region 228 1144 . + 0 ID=BBa_E0432;Name=BBa_E0432 BBa_I12013 . engineered_region 246 1007 . + 0 ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432 BBa_I12013 . stop_codon 1002 1007 . + 0 ID=annotation2159;Name=2;Parent=BBa_E0032 BBa_I12013 . tag 963 1001 . + 0 ID=annotation2160;Name=SsrA;Parent=BBa_E0032 BBa_I12013 . engineered_region 246 1007 . + 0 ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032 BBa_I12013 . CDS 246 1007 . + 0 ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032 BBa_I12013 . sequence_alteration 487 487 . + 0 ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032 BBa_I12013 . ribosome_entry_site 228 239 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432 BBa_I12013 . conserved_region 232 235 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_I12013 . terminator 1016 1095 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432 BBa_I12013 . engineered_region 1016 1095 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_I12013 . stem_loop 1027 1070 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_I12013 . terminator 1104 1144 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432 BBa_I12013 . stem_loop 1111 1130 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_I12013 . engineered_region 1104 1144 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_I12013 . stop_codon 1137 1137 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_I12013 . polyA_site 1131 1144 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_I12013 . terminator 1 129 . + 0 ID=BBa_B0025;Name=BBa_B0025 BBa_I12013 . engineered_region 1 41 . + 0 ID=annotation369702;Name=B0012;Parent=BBa_B0025 BBa_I12013 . engineered_region 50 129 . + 0 ID=annotation369703;Name=B0010;Parent=BBa_B0025 BBa_I12013 . promoter 138 219 . + 0 ID=BBa_I12006;Name=BBa_I12006 BBa_I12013 . non_covalent_binding_site 170 186 . + 0 ID=annotation786518;Name=OR2 lambda;Parent=BBa_I12006 BBa_I12013 . non_covalent_binding_site 146 162 . + 0 ID=annotation786500;Name=OR1 lambda;Parent=BBa_I12006 BBa_I12013 . non_covalent_binding_site 193 206 . + 0 ID=annotation786365;Name=OR1 434;Parent=BBa_I12006 BBa_I12013 . promoter 208 213 . + 0 ID=annotation837228;Name=-10;Parent=BBa_I12006 BBa_I12013 . promoter 185 190 . + 0 ID=annotation837284;Name=-35;Parent=BBa_I12006 >BBa_I12013 tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtactagaggcaaccattatcaccgccaga ggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagagaaagaggaga aatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaa cggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcacc accggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccg accacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaagga cgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatc gacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccg acaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgacca ctaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctg agcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatgg acgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaa taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca cactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcga aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg gcctttctgcgtttata