##gff-version 3
##sequence-region BBa_I0424 1 2700
BBa_I0424	.	CDS	81	842	.	+	0	ID=BBa_C0071;Name=BBa_C0071
BBa_I0424	.	CDS	81	803	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_I0424	.	engineered_tag	843	867	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_I0424	.	tag	804	836	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_I0424	.	terminator	2660	2700	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I0424	.	stop_codon	2693	2693	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I0424	.	stem_loop	2667	2686	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I0424	.	polyA_site	2687	2700	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I0424	.	engineered_region	2660	2700	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I0424	.	CDS	894	1616	.	+	0	ID=BBa_E0020;Name=BBa_E0020
BBa_I0424	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I0424	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I0424	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I0424	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I0424	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I0424	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I0424	.	terminator	1713	1753	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I0424	.	stop_codon	1746	1746	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I0424	.	stem_loop	1720	1739	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I0424	.	polyA_site	1740	1753	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I0424	.	engineered_region	1713	1753	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I0424	.	terminator	2572	2651	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I0424	.	stem_loop	2583	2626	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I0424	.	engineered_region	2572	2651	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I0424	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I0424	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I0424	.	CDS	1841	2563	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_I0424	.	ribosome_entry_site	1823	1834	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I0424	.	conserved_region	1827	1830	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I0424	.	ribosome_entry_site	876	887	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I0424	.	conserved_region	880	883	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I0424	.	terminator	1625	1704	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I0424	.	stem_loop	1636	1679	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I0424	.	engineered_region	1625	1704	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I0424	.	promoter	1762	1814	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_I0424	.	start_codon	1814	1814	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_I0424	.	promoter	1780	1785	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_I0424	.	promoter	1803	1808	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_I0424	.	non_covalent_binding_site	1762	1781	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
>BBa_I0424
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggc
gtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccct
tcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggc
cgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccgg
atgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctca
gcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcat
gatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgc
gaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagca
cggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggc
gctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta
ctagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagc
tggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccct
gaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgc
ttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgca
ccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcat
cgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaac
gtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcg
tgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgag
cacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccggg
atcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaa
gactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggc
ctttctgcgtttatatactagagtcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctac
tagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagct
ggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctg
aagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgct
tcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcac
catcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatc
gagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacg
tctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgt
gcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagc
taccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccggga
tcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttata