##gff-version 3
##sequence-region BBa_I0416 1 940
BBa_I0416	.	ribosome_entry_site	64	74	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_I0416	.	engineered_region	64	74	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_I0416	.	ribosome_entry_site	64	74	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_I0416	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_I0416	.	CDS	81	803	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_I0416	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I0416	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I0416	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I0416	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I0416	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I0416	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I0416	.	terminator	812	940	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I0416	.	terminator	812	891	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I0416	.	stem_loop	823	866	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I0416	.	engineered_region	812	891	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I0416	.	terminator	900	940	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I0416	.	stop_codon	933	933	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I0416	.	polyA_site	927	940	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I0416	.	stem_loop	907	926	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I0416	.	engineered_region	900	940	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_I0416
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggactacta
gatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacat
gaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagca
gaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata