##gff-version 3
##sequence-region BBa_I0413 1 943
BBa_I0413	.	CDS	84	806	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_I0413	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I0413	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I0413	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I0413	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I0413	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I0413	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I0413	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I0413	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I0413	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I0413	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I0413	.	terminator	903	943	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I0413	.	stop_codon	936	936	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I0413	.	stem_loop	910	929	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I0413	.	polyA_site	930	943	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I0413	.	engineered_region	903	943	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I0413	.	terminator	815	894	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I0413	.	stem_loop	826	869	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I0413	.	engineered_region	815	894	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I0413
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggc
cacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccg
gcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgacca
catgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgac
ggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgact
tcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaa
gcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactac
cagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagca
aagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacga
gctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata