##gff-version 3
##sequence-region BBa_I0409 1 1693
BBa_I0409	.	CDS	81	782	.	+	0	ID=BBa_C0076;Name=BBa_C0076
BBa_I0409	.	engineered_tag	783	807	.	+	0	ID=annotation2214001;Name=Help:Barcodes;Parent=BBa_C0076
BBa_I0409	.	tag	744	776	.	+	0	ID=annotation306586;Name=LVA;Parent=BBa_C0076
BBa_I0409	.	CDS	81	743	.	+	0	ID=annotation301035;Name=CinI;Parent=BBa_C0076
BBa_I0409	.	CDS	834	1556	.	+	0	ID=BBa_E0020;Name=BBa_E0020
BBa_I0409	.	terminator	1565	1644	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I0409	.	engineered_region	1565	1644	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I0409	.	stem_loop	1576	1619	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I0409	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I0409	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I0409	.	terminator	1653	1693	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I0409	.	stop_codon	1686	1686	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I0409	.	polyA_site	1680	1693	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I0409	.	stem_loop	1660	1679	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I0409	.	engineered_region	1653	1693	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I0409	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I0409	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I0409	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I0409	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I0409	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I0409	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I0409	.	ribosome_entry_site	816	827	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I0409	.	conserved_region	820	823	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I0409
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaa
gttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccgg
cttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccct
gctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatg
tgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctc
tgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacg
tgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtt
tccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgtt
ccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgata
gtgctagtgtagatccctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtg
gtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgcca
cctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccct
gacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaa
ggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcg
acaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagta
caactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccac
aacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgc
ccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctgga
gttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata