##gff-version 3
##sequence-region BBa_I0405 1 1633
BBa_I0405	.	CDS	774	1496	.	+	0	ID=BBa_E0020;Name=BBa_E0020
BBa_I0405	.	terminator	1505	1584	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I0405	.	stem_loop	1516	1559	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I0405	.	engineered_region	1505	1584	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I0405	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I0405	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I0405	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I0405	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I0405	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I0405	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I0405	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I0405	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I0405	.	CDS	81	722	.	+	0	ID=BBa_C0070;Name=BBa_C0070
BBa_I0405	.	tag	684	716	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_I0405	.	engineered_tag	723	747	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_I0405	.	CDS	81	683	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_I0405	.	terminator	1593	1633	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I0405	.	stop_codon	1626	1626	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I0405	.	engineered_region	1593	1633	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I0405	.	stem_loop	1600	1619	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I0405	.	polyA_site	1620	1633	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I0405	.	ribosome_entry_site	756	767	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I0405	.	conserved_region	760	763	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I0405
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccag
gttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacc
cgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgctta
cctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttac
gctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtg
cttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctggg
tccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctg
ctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttag
tagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggtgagcaagggcga
ggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggc
gagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggc
ccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttctt
caagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgcc
gaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcc
tggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggc
caacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggc
gacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcg
atcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata