iGEM 2018 Distribution Plate 5 Well 23K
type: Collection
persistent identity: iGEM 2018 Plate5 Well23K
display id: iGEM_2018_Plate5_Well23K
version: 1
owned by: myers
member: BBa_K1362052

iGEM 2018 Distribution Plate 5 Well 13N
type: Collection
persistent identity: iGEM 2018 Plate5 Well13N
display id: iGEM_2018_Plate5_Well13N
version: 1
owned by: myers
member: BBa_K1424000

iGEM 2018 Distribution Plate 5 Well 5C
type: Collection
persistent identity: iGEM 2018 Plate5 Well5C
display id: iGEM_2018_Plate5_Well5C
version: 1
owned by: myers
member: BBa_K1333305

iGEM 2018 Distribution Plate 5 Well 12M
type: Collection
persistent identity: iGEM 2018 Plate5 Well12M
display id: iGEM_2018_Plate5_Well12M
version: 1
owned by: myers
member: BBa_K1497011

iGEM 2018 Distribution Plate 5 Well 12E
type: Collection
persistent identity: iGEM 2018 Plate5 Well12E
display id: iGEM_2018_Plate5_Well12E
version: 1
owned by: myers
member: BBa_K1315012

iGEM 2018 Distribution Plate 5 Well 3O
type: Collection
persistent identity: iGEM 2018 Plate5 Well3O
display id: iGEM_2018_Plate5_Well3O
version: 1
owned by: myers
member: BBa_K1333303

iGEM 2018 Distribution Plate 5 Well 10N
type: Collection
persistent identity: iGEM 2018 Plate5 Well10N
display id: iGEM_2018_Plate5_Well10N
version: 1
owned by: myers
member: BBa_K1498000

iGEM 2018 Distribution Plate 5 Well 14O
type: Collection
persistent identity: iGEM 2018 Plate5 Well14O
display id: iGEM_2018_Plate5_Well14O
version: 1
owned by: myers
member: BBa_K1321090

iGEM 2018 Distribution Plate 5 Well 9C
type: Collection
persistent identity: iGEM 2018 Plate5 Well9C
display id: iGEM_2018_Plate5_Well9C
version: 1
owned by: myers
member: BBa_K1554000

iGEM 2018 Distribution Plate 5 Well 9J
type: Collection
persistent identity: iGEM 2018 Plate5 Well9J
display id: iGEM_2018_Plate5_Well9J
version: 1
owned by: myers
member: BBa_K1445001

iGEM 2018 Distribution Plate 5 Well 24F
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persistent identity: iGEM 2018 Plate5 Well24F
display id: iGEM_2018_Plate5_Well24F
version: 1
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member: BBa_K1375002

iGEM 2018 Distribution Plate 5 Well 7O
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persistent identity: iGEM 2018 Plate5 Well7O
display id: iGEM_2018_Plate5_Well7O
version: 1
owned by: myers
member: BBa_K1420003

iGEM 2018 Distribution Plate 5 Well 6L
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persistent identity: iGEM 2018 Plate5 Well6L
display id: iGEM_2018_Plate5_Well6L
version: 1
owned by: myers
member: BBa_K1489002

iGEM 2018 Distribution Plate 5 Well 18C
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persistent identity: iGEM 2018 Plate5 Well18C
display id: iGEM_2018_Plate5_Well18C
version: 1
owned by: myers
member: BBa_K1321341

iGEM 2018 Distribution Plate 5 Well 1M
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persistent identity: iGEM 2018 Plate5 Well1M
display id: iGEM_2018_Plate5_Well1M
version: 1
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member: BBa_K1333101

iGEM 2018 Distribution Plate 5 Well 21F
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persistent identity: iGEM 2018 Plate5 Well21F
display id: iGEM_2018_Plate5_Well21F
version: 1
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member: BBa_K1343020

iGEM 2018 Distribution Plate 5 Well 5A
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persistent identity: iGEM 2018 Plate5 Well5A
display id: iGEM_2018_Plate5_Well5A
version: 1
owned by: myers
member: BBa_K1333304

iGEM 2018 Distribution Plate 5 Well 22D
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persistent identity: iGEM 2018 Plate5 Well22D
display id: iGEM_2018_Plate5_Well22D
version: 1
owned by: myers
member: BBa_K1499501

iGEM 2018 Distribution Plate 5 Well 13O
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persistent identity: iGEM 2018 Plate5 Well13O
display id: iGEM_2018_Plate5_Well13O
version: 1
owned by: myers
member: BBa_K1415009

iGEM 2018 Distribution Plate 5 Well 19N
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persistent identity: iGEM 2018 Plate5 Well19N
display id: iGEM_2018_Plate5_Well19N
version: 1
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member: aGal

iGEM 2018 Distribution Plate 5 Well 19E
type: Collection
persistent identity: iGEM 2018 Plate5 Well19E
display id: iGEM_2018_Plate5_Well19E
version: 1
owned by: myers
member: BBa_K1493802

iGEM 2018 Distribution Plate 5 Well 9K
type: Collection
persistent identity: iGEM 2018 Plate5 Well9K
display id: iGEM_2018_Plate5_Well9K
version: 1
owned by: myers
member: BBa_K1330001

iGEM 2018 Distribution Plate 5 Well 19C
type: Collection
persistent identity: iGEM 2018 Plate5 Well19C
display id: iGEM_2018_Plate5_Well19C
version: 1
owned by: myers
member: BBa_K1493801

iGEM 2018 Distribution Plate 5 Well 19P
type: Collection
persistent identity: iGEM 2018 Plate5 Well19P
display id: iGEM_2018_Plate5_Well19P
version: 1
owned by: myers
member: Intein

iGEM 2018 Distribution Plate 5 Well 8D
type: Collection
persistent identity: iGEM 2018 Plate5 Well8D
display id: iGEM_2018_Plate5_Well8D
version: 1
owned by: myers
member: BBa_K1465302

iGEM 2018 Distribution Plate 5 Well 18E
type: Collection
persistent identity: iGEM 2018 Plate5 Well18E
display id: iGEM_2018_Plate5_Well18E
version: 1
owned by: myers
member: BBa_K1321342

iGEM 2018 Distribution Plate 5 Well 21E
type: Collection
persistent identity: iGEM 2018 Plate5 Well21E
display id: iGEM_2018_Plate5_Well21E
version: 1
owned by: myers
member: BBa_K1493501

iGEM 2018 Distribution Plate 5 Well 24B
type: Collection
persistent identity: iGEM 2018 Plate5 Well24B
display id: iGEM_2018_Plate5_Well24B
version: 1
owned by: myers
member: BBa_K1393003

iGEM 2018 Distribution Plate 5 Well 7A
type: Collection
persistent identity: iGEM 2018 Plate5 Well7A
display id: iGEM_2018_Plate5_Well7A
version: 1
owned by: myers
member: BBa_K1333315

iGEM 2018 Distribution Plate 5 Well 8G
type: Collection
persistent identity: iGEM 2018 Plate5 Well8G
display id: iGEM_2018_Plate5_Well8G
version: 1
owned by: myers
member: CircSortA*

iGEM 2018 Distribution Plate 5 Well 5N
type: Collection
persistent identity: iGEM 2018 Plate5 Well5N
display id: iGEM_2018_Plate5_Well5N
version: 1
owned by: myers
member: BBa_K1365020

iGEM 2018 Distribution Plate 5 Well 21M
type: Collection
persistent identity: iGEM 2018 Plate5 Well21M
display id: iGEM_2018_Plate5_Well21M
version: 1
owned by: myers
member: BBa_K1362011

iGEM 2018 Distribution Plate 5 Well 1O
type: Collection
persistent identity: iGEM 2018 Plate5 Well1O
display id: iGEM_2018_Plate5_Well1O
version: 1
owned by: myers
member: BBa_K1333102

iGEM 2018 Distribution Plate 5 Well 17H
type: Collection
persistent identity: iGEM 2018 Plate5 Well17H
display id: iGEM_2018_Plate5_Well17H
version: 1
owned by: myers
member: 1018

iGEM 2018 Distribution Plate 5 Well 10C
type: Collection
persistent identity: iGEM 2018 Plate5 Well10C
display id: iGEM_2018_Plate5_Well10C
version: 1
owned by: myers
member: BBa_K1315001

iGEM 2018 Distribution Plate 5 Well 23F
type: Collection
persistent identity: iGEM 2018 Plate5 Well23F
display id: iGEM_2018_Plate5_Well23F
version: 1
owned by: myers
member: BBa_K1336003

iGEM 2018 Distribution Plate 5 Well 23J
type: Collection
persistent identity: iGEM 2018 Plate5 Well23J
display id: iGEM_2018_Plate5_Well23J
version: 1
owned by: myers
member: BBa_K1351000

iGEM 2018 Distribution Plate 5 Well 18H
type: Collection
persistent identity: iGEM 2018 Plate5 Well18H
display id: iGEM_2018_Plate5_Well18H
version: 1
owned by: myers
member: BBa_K1465307

iGEM 2018 Distribution Plate 5 Well 5B
type: Collection
persistent identity: iGEM 2018 Plate5 Well5B
display id: iGEM_2018_Plate5_Well5B
version: 1
owned by: myers
member: BBa_K1478000

iGEM 2018 Distribution Plate 5 Well 7H
type: Collection
persistent identity: iGEM 2018 Plate5 Well7H
display id: iGEM_2018_Plate5_Well7H
version: 1
owned by: myers
member: BBa_K1399005

iGEM 2018 Distribution Plate 5 Well 19I
type: Collection
persistent identity: iGEM 2018 Plate5 Well19I
display id: iGEM_2018_Plate5_Well19I
version: 1
owned by: myers
member: BBa_K1493804

iGEM 2018 Distribution Plate 5 Well 9O
type: Collection
persistent identity: iGEM 2018 Plate5 Well9O
display id: iGEM_2018_Plate5_Well9O
version: 1
owned by: myers
member: BBa_K1319004

iGEM 2018 Distribution Plate 5 Well 21I
type: Collection
persistent identity: iGEM 2018 Plate5 Well21I
display id: iGEM_2018_Plate5_Well21I
version: 1
owned by: myers
member: Mj TyrRS

iGEM 2018 Distribution Plate 5 Well 15J
type: Collection
persistent identity: iGEM 2018 Plate5 Well15J
display id: iGEM_2018_Plate5_Well15J
version: 1
owned by: myers
member: BBa_K1460003

iGEM 2018 Distribution Plate 5 Well 20D
type: Collection
persistent identity: iGEM 2018 Plate5 Well20D
display id: iGEM_2018_Plate5_Well20D
version: 1
owned by: myers
member: BBa_K1357009

iGEM 2018 Distribution Plate 5 Well 1I
type: Collection
persistent identity: iGEM 2018 Plate5 Well1I
display id: iGEM_2018_Plate5_Well1I
version: 1
owned by: myers
member: BBa_K1404008

iGEM 2018 Distribution Plate 5 Well 21H
type: Collection
persistent identity: iGEM 2018 Plate5 Well21H
display id: iGEM_2018_Plate5_Well21H
version: 1
owned by: myers
member: BBa_K1343021

iGEM 2018 Distribution Plate 5 Well 16A
type: Collection
persistent identity: iGEM 2018 Plate5 Well16A
display id: iGEM_2018_Plate5_Well16A
version: 1
owned by: myers
member: BBa_K1321092

iGEM 2018 Distribution Plate 5 Well 21N
type: Collection
persistent identity: iGEM 2018 Plate5 Well21N
display id: iGEM_2018_Plate5_Well21N
version: 1
owned by: myers
member: BBa_K1355002

iGEM 2018 Distribution Plate 5 Well 10D
type: Collection
persistent identity: iGEM 2018 Plate5 Well10D
display id: iGEM_2018_Plate5_Well10D
version: 1
owned by: myers
member: BBa_K1323012

iGEM 2018 Distribution Plate 5 Well 9G
type: Collection
persistent identity: iGEM 2018 Plate5 Well9G
display id: iGEM_2018_Plate5_Well9G
version: 1
owned by: myers
member: BBa_K1554002

iGEM 2018 Distribution Plate 5 Well 20E
type: Collection
persistent identity: iGEM 2018 Plate5 Well20E
display id: iGEM_2018_Plate5_Well20E
version: 1
owned by: myers
member: BBa_K1321351

iGEM 2018 Distribution Plate 5 Well 17J
type: Collection
persistent identity: iGEM 2018 Plate5 Well17J
display id: iGEM_2018_Plate5_Well17J
version: 1
owned by: myers
member: BBa_K1472601

iGEM 2018 Distribution Plate 5 Well 6H
type: Collection
persistent identity: iGEM 2018 Plate5 Well6H
display id: iGEM_2018_Plate5_Well6H
version: 1
owned by: myers
member: BBa_K1351012

iGEM 2018 Distribution Plate 5 Well 20H
type: Collection
persistent identity: iGEM 2018 Plate5 Well20H
display id: iGEM_2018_Plate5_Well20H
version: 1
owned by: myers
member: BBa_K1450003

iGEM 2018 Distribution Plate 5 Well 8N
type: Collection
persistent identity: iGEM 2018 Plate5 Well8N
display id: iGEM_2018_Plate5_Well8N
version: 1
owned by: myers
member: BBa_K1323009

iGEM 2018 Distribution Plate 5 Well 17B
type: Collection
persistent identity: iGEM 2018 Plate5 Well17B
display id: iGEM_2018_Plate5_Well17B
version: 1
owned by: myers
member: REDOX

iGEM 2018 Distribution Plate 5 Well 8O
type: Collection
persistent identity: iGEM 2018 Plate5 Well8O
display id: iGEM_2018_Plate5_Well8O
version: 1
owned by: myers
member: BBa_K1362202

iGEM 2018 Distribution Plate 5 Well 1G
type: Collection
persistent identity: iGEM 2018 Plate5 Well1G
display id: iGEM_2018_Plate5_Well1G
version: 1
owned by: myers
member: BBa_K1404007

iGEM 2018 Distribution Plate 5 Well 9I
type: Collection
persistent identity: iGEM 2018 Plate5 Well9I
display id: iGEM_2018_Plate5_Well9I
version: 1
owned by: myers
member: BBa_K1330000

iGEM 2018 Distribution Plate 5 Well 18F
type: Collection
persistent identity: iGEM 2018 Plate5 Well18F
display id: iGEM_2018_Plate5_Well18F
version: 1
owned by: myers
member: BBa_K1465306

iGEM 2018 Distribution Plate 5 Well 5D
type: Collection
persistent identity: iGEM 2018 Plate5 Well5D
display id: iGEM_2018_Plate5_Well5D
version: 1
owned by: myers
member: BBa_K1478001

iGEM 2018 Distribution Plate 5 Well 9L
type: Collection
persistent identity: iGEM 2018 Plate5 Well9L
display id: iGEM_2018_Plate5_Well9L
version: 1
owned by: myers
member: BBa_K1331001

iGEM 2018 Distribution Plate 5 Well 12K
type: Collection
persistent identity: iGEM 2018 Plate5 Well12K
display id: iGEM_2018_Plate5_Well12K
version: 1
owned by: myers
member: BBa_K1497010

iGEM 2018 Distribution Plate 5 Well 12L
type: Collection
persistent identity: iGEM 2018 Plate5 Well12L
display id: iGEM_2018_Plate5_Well12L
version: 1
owned by: myers
member: BBa_K1321090

iGEM 2018 Distribution Plate 5 Well 24K
type: Collection
persistent identity: iGEM 2018 Plate5 Well24K
display id: iGEM_2018_Plate5_Well24K
version: 1
owned by: myers
member: BBa_K1321100

iGEM 2018 Distribution Plate 5
type: Collection
persistent identity: iGEM 2018 Plate5
display id: iGEM_2018_Plate5
version: 1
owned by: myers
member: iGEM 2018 Distribution Plate 5 Well 23K
member: BBa_K1401601
member: BBa_K1509000
member: iGEM 2018 Distribution Plate 5 Well 3K
member: iGEM 2018 Distribution Plate 5 Well 5B
member: iGEM 2018 Distribution Plate 5 Well 8B
member: iGEM 2018 Distribution Plate 5 Well 2B
member: iGEM 2018 Distribution Plate 5 Well 9K
member: iGEM 2018 Distribution Plate 5 Well 11L
member: iGEM 2018 Distribution Plate 5 Well 18G
member: BBa_K1325002
member: BBa_K1333305
member: BBa_K1497026
member: iGEM 2018 Distribution Plate 5 Well 6K
member: iGEM 2018 Distribution Plate 5 Well 22B
member: BBa_K1415103
member: BBa_K1415008
member: BBa_K1497031
member: iGEM 2018 Distribution Plate 5 Well 14L
member: BBa_K1351003
member: BBa_K1343022
member: iGEM 2018 Distribution Plate 5 Well 12G
member: iGEM 2018 Distribution Plate 5 Well 15G
member: iGEM 2018 Distribution Plate 5 Well 21G
member: TerDnaE3(C
member: 1GateNpuE
member: BBa_K1351033
member: BBa_K1321356
member: BBa_K1424000
member: BBa_K1493300
member: iGEM 2018 Distribution Plate 5 Well 5O
member: 1NpuE(N*)
member: iGEM 2018 Distribution Plate 5 Well 2O
member: BBa_K1321122
member: BBa_K1351032
member: iGEM 2018 Distribution Plate 5 Well 8O
member: iGEM 2018 Distribution Plate 5 Well 16C
member: BBa_K1493801
member: iGEM 2018 Distribution Plate 5 Well 19C
member: BBa_K1321152
member: BBa_K1333102
member: BBa_K1438002
member: iGEM 2018 Distribution Plate 5 Well 13C
member: BBa_K1486022
member: BBa_K1351027
member: iGEM 2018 Distribution Plate 5 Well 10C
member: BBa_K1460004
member: BBa_K1333304
member: iGEM 2018 Distribution Plate 5 Well 18H
member: BBa_K1323002
member: iGEM 2018 Distribution Plate 5 Well 16B
member: BBa_K1321094
member: iGEM 2018 Distribution Plate 5 Well 9J
member: iGEM 2018 Distribution Plate 5 Well 6J
member: iGEM 2018 Distribution Plate 5 Well 3J
member: BBa_K1499000
member: iGEM 2018 Distribution Plate 5 Well 20L
member: BBa_K1351028
member: iGEM 2018 Distribution Plate 5 Well 10B
member: iGEM 2018 Distribution Plate 5 Well 15H
member: BBa_K1529311
member: iGEM 2018 Distribution Plate 5 Well 19B
member: BBa_K1415009
member: BBa_K1497032
member: iGEM 2018 Distribution Plate 5 Well 12H
member: iGEM 2018 Distribution Plate 5 Well 13B
member: BBa_K1505002
member: BBa_K1351002
member: iGEM 2018 Distribution Plate 5 Well 2P
member: iGEM 2018 Distribution Plate 5 Well 23L
member: BBa_K1467204
member: BBa_K1362011
member: iGEM 2018 Distribution Plate 5 Well 5P
member: BBa_K1333306

iGEM 2018 Distribution Plate 5 Well 11C
type: Collection
persistent identity: iGEM 2018 Plate5 Well11C
display id: iGEM_2018_Plate5_Well11C
version: 1
owned by: myers
member: BBa_K1319020

iGEM 2018 Distribution Plate 5 Well 14H
type: Collection
persistent identity: iGEM 2018 Plate5 Well14H
display id: iGEM_2018_Plate5_Well14H
version: 1
owned by: myers
member: BBa_K1403013

iGEM 2018 Distribution Plate 5 Well 14C
type: Collection
persistent identity: iGEM 2018 Plate5 Well14C
display id: iGEM_2018_Plate5_Well14C
version: 1
owned by: myers
member: BBa_K1497026

iGEM 2018 Distribution Plate 5 Well 3P
type: Collection
persistent identity: iGEM 2018 Plate5 Well3P
display id: iGEM_2018_Plate5_Well3P
version: 1
owned by: myers
member: BBa_K1445000

iGEM 2018 Distribution Plate 5 Well 13M
type: Collection
persistent identity: iGEM 2018 Plate5 Well13M
display id: iGEM_2018_Plate5_Well13M
version: 1
owned by: myers
member: BBa_K1415008

iGEM 2018 Distribution Plate 5 Well 23L
type: Collection
persistent identity: iGEM 2018 Plate5 Well23L
display id: iGEM_2018_Plate5_Well23L
version: 1
owned by: myers
member: BBa_K1351001

iGEM 2018 Distribution Plate 5 Well 16H
type: Collection
persistent identity: iGEM 2018 Plate5 Well16H
display id: iGEM_2018_Plate5_Well16H
version: 1
owned by: myers
member: BBa_K1321112

iGEM 2018 Distribution Plate 5 Well 1A
type: Collection
persistent identity: iGEM 2018 Plate5 Well1A
display id: iGEM_2018_Plate5_Well1A
version: 1
owned by: myers
member: BBa_K1404001

iGEM 2018 Distribution Plate 5 Well 19G
type: Collection
persistent identity: iGEM 2018 Plate5 Well19G
display id: iGEM_2018_Plate5_Well19G
version: 1
owned by: myers
member: BBa_K1493803

iGEM 2018 Distribution Plate 5 Well 21K
type: Collection
persistent identity: iGEM 2018 Plate5 Well21K
display id: iGEM_2018_Plate5_Well21K
version: 1
owned by: myers
member: CircNpu

iGEM 2018 Distribution Plate 5 Well 15B
type: Collection
persistent identity: iGEM 2018 Plate5 Well15B
display id: iGEM_2018_Plate5_Well15B
version: 1
owned by: myers
member: nadE

iGEM 2018 Distribution Plate 5 Well 22J
type: Collection
persistent identity: iGEM 2018 Plate5 Well22J
display id: iGEM_2018_Plate5_Well22J
version: 1
owned by: myers
member: BBa_K1401008

iGEM 2018 Distribution Plate 5 Well 15P
type: Collection
persistent identity: iGEM 2018 Plate5 Well15P
display id: iGEM_2018_Plate5_Well15P
version: 1
owned by: myers
member: SAMmt

iGEM 2018 Distribution Plate 5 Well 11P
type: Collection
persistent identity: iGEM 2018 Plate5 Well11P
display id: iGEM_2018_Plate5_Well11P
version: 1
owned by: myers
member: BBa_K1350005

iGEM 2018 Distribution Plate 5 Well 11L
type: Collection
persistent identity: iGEM 2018 Plate5 Well11L
display id: iGEM_2018_Plate5_Well11L
version: 1
owned by: myers
member: BBa_K1334000

iGEM 2018 Distribution Plate 5 Well 1B
type: Collection
persistent identity: iGEM 2018 Plate5 Well1B
display id: iGEM_2018_Plate5_Well1B
version: 1
owned by: myers
member: BBa_K1491005

iGEM 2018 Distribution Plate 5 Well 7L
type: Collection
persistent identity: iGEM 2018 Plate5 Well7L
display id: iGEM_2018_Plate5_Well7L
version: 1
owned by: myers
member: BBa_K1399006

iGEM 2018 Distribution Plate 5 Well 1K
type: Collection
persistent identity: iGEM 2018 Plate5 Well1K
display id: iGEM_2018_Plate5_Well1K
version: 1
owned by: myers
member: BBa_K1333005

iGEM 2018 Distribution Plate 5 Well 7M
type: Collection
persistent identity: iGEM 2018 Plate5 Well7M
display id: iGEM_2018_Plate5_Well7M
version: 1
owned by: myers
member: BBa_K1420002

iGEM 2018 Distribution Plate 5 Well 12H
type: Collection
persistent identity: iGEM 2018 Plate5 Well12H
display id: iGEM_2018_Plate5_Well12H
version: 1
owned by: myers
member: BBa_K1365998

iGEM 2018 Distribution Plate 5 Well 9P
type: Collection
persistent identity: iGEM 2018 Plate5 Well9P
display id: iGEM_2018_Plate5_Well9P
version: 1
owned by: myers
member: BBa_K1331002

iGEM 2018 Distribution Plate 5 Well 8L
type: Collection
persistent identity: iGEM 2018 Plate5 Well8L
display id: iGEM_2018_Plate5_Well8L
version: 1
owned by: myers
member: BBa_K1323002

iGEM 2018 Distribution Plate 5 Well 10F
type: Collection
persistent identity: iGEM 2018 Plate5 Well10F
display id: iGEM_2018_Plate5_Well10F
version: 1
owned by: myers
member: BBa_K1323013

iGEM 2018 Distribution Plate 5 Well 6J
type: Collection
persistent identity: iGEM 2018 Plate5 Well6J
display id: iGEM_2018_Plate5_Well6J
version: 1
owned by: myers
member: BBa_K1351013

iGEM 2018 Distribution Plate 5 Well 15F
type: Collection
persistent identity: iGEM 2018 Plate5 Well15F
display id: iGEM_2018_Plate5_Well15F
version: 1
owned by: myers
member: BBa_K1393001

iGEM 2018 Distribution Plate 5 Well 15O
type: Collection
persistent identity: iGEM 2018 Plate5 Well15O
display id: iGEM_2018_Plate5_Well15O
version: 1
owned by: myers
member: BBa_K1415108

iGEM 2018 Distribution Plate 5 Well 20C
type: Collection
persistent identity: iGEM 2018 Plate5 Well20C
display id: iGEM_2018_Plate5_Well20C
version: 1
owned by: myers
member: BBa_K1321349

iGEM 2018 Distribution Plate 5 Well 6O
type: Collection
persistent identity: iGEM 2018 Plate5 Well6O
display id: iGEM_2018_Plate5_Well6O
version: 1
owned by: myers
member: BBa_K1362160

iGEM 2018 Distribution Plate 5 Well 21A
type: Collection
persistent identity: iGEM 2018 Plate5 Well21A
display id: iGEM_2018_Plate5_Well21A
version: 1
owned by: myers
member: BBa_K1493540

iGEM 2018 Distribution Plate 5 Well 2D
type: Collection
persistent identity: iGEM 2018 Plate5 Well2D
display id: iGEM_2018_Plate5_Well2D
version: 1
owned by: myers
member: BBa_K1351003

iGEM 2018 Distribution Plate 5 Well 16E
type: Collection
persistent identity: iGEM 2018 Plate5 Well16E
display id: iGEM_2018_Plate5_Well16E
version: 1
owned by: myers
member: BBa_K1321200

iGEM 2018 Distribution Plate 5 Well 2E
type: Collection
persistent identity: iGEM 2018 Plate5 Well2E
display id: iGEM_2018_Plate5_Well2E
version: 1
owned by: myers
member: BBa_K1362055

iGEM 2018 Distribution Plate 5 Well 11E
type: Collection
persistent identity: iGEM 2018 Plate5 Well11E
display id: iGEM_2018_Plate5_Well11E
version: 1
owned by: myers
member: BBa_K1319008

iGEM 2018 Distribution Plate 5 Well 19F
type: Collection
persistent identity: iGEM 2018 Plate5 Well19F
display id: iGEM_2018_Plate5_Well19F
version: 1
owned by: myers
member: BBa_K1349004

iGEM 2018 Distribution Plate 5 Well 9E
type: Collection
persistent identity: iGEM 2018 Plate5 Well9E
display id: iGEM_2018_Plate5_Well9E
version: 1
owned by: myers
member: BBa_K1554001

iGEM 2018 Distribution Plate 5 Well 18G
type: Collection
persistent identity: iGEM 2018 Plate5 Well18G
display id: iGEM_2018_Plate5_Well18G
version: 1
owned by: myers
member: BBa_K1321343

iGEM 2018 Distribution Plate 5 Well 20B
type: Collection
persistent identity: iGEM 2018 Plate5 Well20B
display id: iGEM_2018_Plate5_Well20B
version: 1
owned by: myers
member: BBa_K1357008

iGEM 2018 Distribution Plate 5 Well 19O
type: Collection
persistent identity: iGEM 2018 Plate5 Well19O
display id: iGEM_2018_Plate5_Well19O
version: 1
owned by: myers
member: BBa_K1493520

iGEM 2018 Distribution Plate 5 Well 17A
type: Collection
persistent identity: iGEM 2018 Plate5 Well17A
display id: iGEM_2018_Plate5_Well17A
version: 1
owned by: myers
member: BBa_K1415109

iGEM 2018 Distribution Plate 5 Well 4F
type: Collection
persistent identity: iGEM 2018 Plate5 Well4F
display id: iGEM_2018_Plate5_Well4F
version: 1
owned by: myers
member: BBa_K1351027

iGEM 2018 Distribution Plate 5 Well 15N
type: Collection
persistent identity: iGEM 2018 Plate5 Well15N
display id: iGEM_2018_Plate5_Well15N
version: 1
owned by: myers
member: GAFP-1

iGEM 2018 Distribution Plate 5 Well 18D
type: Collection
persistent identity: iGEM 2018 Plate5 Well18D
display id: iGEM_2018_Plate5_Well18D
version: 1
owned by: myers
member: BBa_K1508003

iGEM 2018 Distribution Plate 5 Well 16G
type: Collection
persistent identity: iGEM 2018 Plate5 Well16G
display id: iGEM_2018_Plate5_Well16G
version: 1
owned by: myers
member: BBa_K1321005

iGEM 2018 Distribution Plate 5 Well 16B
type: Collection
persistent identity: iGEM 2018 Plate5 Well16B
display id: iGEM_2018_Plate5_Well16B
version: 1
owned by: myers
member: BBa_K1321105

iGEM 2018 Distribution Plate 5 Well 16N
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persistent identity: iGEM 2018 Plate5 Well16N
display id: iGEM_2018_Plate5_Well16N
version: 1
owned by: myers
member: BBa_K1470002

iGEM 2018 Distribution Plate 5 Well 5F
type: Collection
persistent identity: iGEM 2018 Plate5 Well5F
display id: iGEM_2018_Plate5_Well5F
version: 1
owned by: myers
member: BBa_K1467201

iGEM 2018 Distribution Plate 5 Well 7G
type: Collection
persistent identity: iGEM 2018 Plate5 Well7G
display id: iGEM_2018_Plate5_Well7G
version: 1
owned by: myers
member: BBa_K1316015

iGEM 2018 Distribution Plate 5 Well 10H
type: Collection
persistent identity: iGEM 2018 Plate5 Well10H
display id: iGEM_2018_Plate5_Well10H
version: 1
owned by: myers
member: BBa_K1323014

iGEM 2018 Distribution Plate 5 Well 3M
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persistent identity: iGEM 2018 Plate5 Well3M
display id: iGEM_2018_Plate5_Well3M
version: 1
owned by: myers
member: BBa_K1333301

iGEM 2018 Distribution Plate 5 Well 22E
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persistent identity: iGEM 2018 Plate5 Well22E
display id: iGEM_2018_Plate5_Well22E
version: 1
owned by: myers
member: BBa_K1321121

iGEM 2018 Distribution Plate 5 Well 2F
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persistent identity: iGEM 2018 Plate5 Well2F
display id: iGEM_2018_Plate5_Well2F
version: 1
owned by: myers
member: BBa_K1351004

iGEM 2018 Distribution Plate 5 Well 21B
type: Collection
persistent identity: iGEM 2018 Plate5 Well21B
display id: iGEM_2018_Plate5_Well21B
version: 1
owned by: myers
member: BBa_K1446002

iGEM 2018 Distribution Plate 5 Well 3K
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persistent identity: iGEM 2018 Plate5 Well3K
display id: iGEM_2018_Plate5_Well3K
version: 1
owned by: myers
member: BBa_K1333300

iGEM 2018 Distribution Plate 5 Well 20A
type: Collection
persistent identity: iGEM 2018 Plate5 Well20A
display id: iGEM_2018_Plate5_Well20A
version: 1
owned by: myers
member: BBa_K1321348

iGEM 2018 Distribution Plate 5 Well 23C
type: Collection
persistent identity: iGEM 2018 Plate5 Well23C
display id: iGEM_2018_Plate5_Well23C
version: 1
owned by: myers
member: BBa_K1362021

iGEM 2018 Distribution Plate 5 Well 7P
type: Collection
persistent identity: iGEM 2018 Plate5 Well7P
display id: iGEM_2018_Plate5_Well7P
version: 1
owned by: myers
member: BBa_K1399008

iGEM 2018 Distribution Plate 5 Well 6P
type: Collection
persistent identity: iGEM 2018 Plate5 Well6P
display id: iGEM_2018_Plate5_Well6P
version: 1
owned by: myers
member: BBa_K1510002

iGEM 2018 Distribution Plate 5 Well 7I
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persistent identity: iGEM 2018 Plate5 Well7I
display id: iGEM_2018_Plate5_Well7I
version: 1
owned by: myers
member: BBa_K1420000

iGEM 2018 Distribution Plate 5 Well 18L
type: Collection
persistent identity: iGEM 2018 Plate5 Well18L
display id: iGEM_2018_Plate5_Well18L
version: 1
owned by: myers
member: BBa_K1333107

iGEM 2018 Distribution Plate 5 Well 20O
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persistent identity: iGEM 2018 Plate5 Well20O
display id: iGEM_2018_Plate5_Well20O
version: 1
owned by: myers
member: BBa_K1321358

iGEM 2018 Distribution Plate 5 Well 8F
type: Collection
persistent identity: iGEM 2018 Plate5 Well8F
display id: iGEM_2018_Plate5_Well8F
version: 1
owned by: myers
member: BBa_K1456002

iGEM 2018 Distribution Plate 5 Well 4D
type: Collection
persistent identity: iGEM 2018 Plate5 Well4D
display id: iGEM_2018_Plate5_Well4D
version: 1
owned by: myers
member: BBa_K1351026

iGEM 2018 Distribution Plate 5 Well 7C
type: Collection
persistent identity: iGEM 2018 Plate5 Well7C
display id: iGEM_2018_Plate5_Well7C
version: 1
owned by: myers
member: BBa_K1333318

iGEM 2018 Distribution Plate 5 Well 10E
type: Collection
persistent identity: iGEM 2018 Plate5 Well10E
display id: iGEM_2018_Plate5_Well10E
version: 1
owned by: myers
member: BBa_K1315003

iGEM 2018 Distribution Plate 5 Well 5P
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persistent identity: iGEM 2018 Plate5 Well5P
display id: iGEM_2018_Plate5_Well5P
version: 1
owned by: myers
member: BBa_K1399000

iGEM 2018 Distribution Plate 5 Well 12O
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persistent identity: iGEM 2018 Plate5 Well12O
display id: iGEM_2018_Plate5_Well12O
version: 1
owned by: myers
member: BBa_K1497013

iGEM 2018 Distribution Plate 5 Well 2H
type: Collection
persistent identity: iGEM 2018 Plate5 Well2H
display id: iGEM_2018_Plate5_Well2H
version: 1
owned by: myers
member: BBa_K1351005

iGEM 2018 Distribution Plate 5 Well 24E
type: Collection
persistent identity: iGEM 2018 Plate5 Well24E
display id: iGEM_2018_Plate5_Well24E
version: 1
owned by: myers
member: BBa_K1321162

iGEM 2018 Distribution Plate 5 Well 2C
type: Collection
persistent identity: iGEM 2018 Plate5 Well2C
display id: iGEM_2018_Plate5_Well2C
version: 1
owned by: myers
member: BBa_K1362054

iGEM 2018 Distribution Plate 5 Well 22G
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persistent identity: iGEM 2018 Plate5 Well22G
display id: iGEM_2018_Plate5_Well22G
version: 1
owned by: myers
member: BBa_K1321122

iGEM 2018 Distribution Plate 5 Well 1H
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persistent identity: iGEM 2018 Plate5 Well1H
display id: iGEM_2018_Plate5_Well1H
version: 1
owned by: myers
member: BBa_K1491015

iGEM 2018 Distribution Plate 5 Well 9M
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persistent identity: iGEM 2018 Plate5 Well9M
display id: iGEM_2018_Plate5_Well9M
version: 1
owned by: myers
member: REACh2

iGEM 2018 Distribution Plate 5 Well 14A
type: Collection
persistent identity: iGEM 2018 Plate5 Well14A
display id: iGEM_2018_Plate5_Well14A
version: 1
owned by: myers
member: BBa_K1497024

iGEM 2018 Distribution Plate 5 Well 7K
type: Collection
persistent identity: iGEM 2018 Plate5 Well7K
display id: iGEM_2018_Plate5_Well7K
version: 1
owned by: myers
member: BBa_K1420001

iGEM 2018 Distribution Plate 5 Well 24H
type: Collection
persistent identity: iGEM 2018 Plate5 Well24H
display id: iGEM_2018_Plate5_Well24H
version: 1
owned by: myers
member: BBa_K1375005

iGEM 2018 Distribution Plate 5 Well 20K
type: Collection
persistent identity: iGEM 2018 Plate5 Well20K
display id: iGEM_2018_Plate5_Well20K
version: 1
owned by: myers
member: BBa_K1321356

iGEM 2018 Distribution Plate 5 Well 21J
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persistent identity: iGEM 2018 Plate5 Well21J
display id: iGEM_2018_Plate5_Well21J
version: 1
owned by: myers
member: BBa_K1343022

iGEM 2018 Distribution Plate 5 Well 11N
type: Collection
persistent identity: iGEM 2018 Plate5 Well11N
display id: iGEM_2018_Plate5_Well11N
version: 1
owned by: myers
member: BBa_K1332001

iGEM 2018 Distribution Plate 5 Well 10K
type: Collection
persistent identity: iGEM 2018 Plate5 Well10K
display id: iGEM_2018_Plate5_Well10K
version: 1
owned by: myers
member: BBa_K1315007

iGEM 2018 Distribution Plate 5 Well 16F
type: Collection
persistent identity: iGEM 2018 Plate5 Well16F
display id: iGEM_2018_Plate5_Well16F
version: 1
owned by: myers
member: BBa_K1321111

iGEM 2018 Distribution Plate 5 Well 7N
type: Collection
persistent identity: iGEM 2018 Plate5 Well7N
display id: iGEM_2018_Plate5_Well7N
version: 1
owned by: myers
member: BBa_K1399007

iGEM 2018 Distribution Plate 5 Well 10A
type: Collection
persistent identity: iGEM 2018 Plate5 Well10A
display id: iGEM_2018_Plate5_Well10A
version: 1
owned by: myers
member: Smt3-mDNMT

iGEM 2018 Distribution Plate 5 Well 23A
type: Collection
persistent identity: iGEM 2018 Plate5 Well23A
display id: iGEM_2018_Plate5_Well23A
version: 1
owned by: myers
member: BBa_K1362020

iGEM 2018 Distribution Plate 5 Well 13B
type: Collection
persistent identity: iGEM 2018 Plate5 Well13B
display id: iGEM_2018_Plate5_Well13B
version: 1
owned by: myers
member: BBa_K1325001

iGEM 2018 Distribution Plate 5 Well 12J
type: Collection
persistent identity: iGEM 2018 Plate5 Well12J
display id: iGEM_2018_Plate5_Well12J
version: 1
owned by: myers
member: BBa_K1365997

iGEM 2018 Distribution Plate 5 Well 3A
type: Collection
persistent identity: iGEM 2018 Plate5 Well3A
display id: iGEM_2018_Plate5_Well3A
version: 1
owned by: myers
member: BBa_K1333105

iGEM 2018 Distribution Plate 5 Well 7J
type: Collection
persistent identity: iGEM 2018 Plate5 Well7J
display id: iGEM_2018_Plate5_Well7J
version: 1
owned by: myers
member: BBa_K1399004

iGEM 2018 Distribution Plate 5 Well 21O
type: Collection
persistent identity: iGEM 2018 Plate5 Well21O
display id: iGEM_2018_Plate5_Well21O
version: 1
owned by: myers
member: BBa_K1362013

iGEM 2018 Distribution Plate 5 Well 6A
type: Collection
persistent identity: iGEM 2018 Plate5 Well6A
display id: iGEM_2018_Plate5_Well6A
version: 1
owned by: myers
member: TerDnaE3(C

iGEM 2018 Distribution Plate 5 Well 12A
type: Collection
persistent identity: iGEM 2018 Plate5 Well12A
display id: iGEM_2018_Plate5_Well12A
version: 1
owned by: myers
member: BBa_K1315010

iGEM 2018 Distribution Plate 5 Well 12G
type: Collection
persistent identity: iGEM 2018 Plate5 Well12G
display id: iGEM_2018_Plate5_Well12G
version: 1
owned by: myers
member: BBa_K1497008

iGEM 2018 Distribution Plate 5 Well 15D
type: Collection
persistent identity: iGEM 2018 Plate5 Well15D
display id: iGEM_2018_Plate5_Well15D
version: 1
owned by: myers
member: BBa_K1365169

iGEM 2018 Distribution Plate 5 Well 23G
type: Collection
persistent identity: iGEM 2018 Plate5 Well23G
display id: iGEM_2018_Plate5_Well23G
version: 1
owned by: myers
member: BBa_K1362050

iGEM 2018 Distribution Plate 5 Well 24G
type: Collection
persistent identity: iGEM 2018 Plate5 Well24G
display id: iGEM_2018_Plate5_Well24G
version: 1
owned by: myers
member: BBa_K1321366

iGEM 2018 Distribution Plate 5 Well 2P
type: Collection
persistent identity: iGEM 2018 Plate5 Well2P
display id: iGEM_2018_Plate5_Well2P
version: 1
owned by: myers
member: BBa_K1351010

iGEM 2018 Distribution Plate 5 Well 17E
type: Collection
persistent identity: iGEM 2018 Plate5 Well17E
display id: iGEM_2018_Plate5_Well17E
version: 1
owned by: myers
member: BBa_K1529311

iGEM 2018 Distribution Plate 5 Well 20F
type: Collection
persistent identity: iGEM 2018 Plate5 Well20F
display id: iGEM_2018_Plate5_Well20F
version: 1
owned by: myers
member: BBa_K1357010

iGEM 2018 Distribution Plate 5 Well 17C
type: Collection
persistent identity: iGEM 2018 Plate5 Well17C
display id: iGEM_2018_Plate5_Well17C
version: 1
owned by: myers
member: BBa_K1529321

iGEM 2018 Distribution Plate 5 Well 16P
type: Collection
persistent identity: iGEM 2018 Plate5 Well16P
display id: iGEM_2018_Plate5_Well16P
version: 1
owned by: myers
member: BBa_K1470007

iGEM 2018 Distribution Plate 5 Well 18P
type: Collection
persistent identity: iGEM 2018 Plate5 Well18P
display id: iGEM_2018_Plate5_Well18P
version: 1
owned by: myers
member: BBa_K577882

iGEM 2018 Distribution Plate 5 Well 16D
type: Collection
persistent identity: iGEM 2018 Plate5 Well16D
display id: iGEM_2018_Plate5_Well16D
version: 1
owned by: myers
member: BBa_K1321110

iGEM 2018 Distribution Plate 5 Well 23O
type: Collection
persistent identity: iGEM 2018 Plate5 Well23O
display id: iGEM_2018_Plate5_Well23O
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 5 Well 10I
type: Collection
persistent identity: iGEM 2018 Plate5 Well10I
display id: iGEM_2018_Plate5_Well10I
version: 1
owned by: myers
member: BBa_K1315006

iGEM 2018 Distribution Plate 5 Well 18J
type: Collection
persistent identity: iGEM 2018 Plate5 Well18J
display id: iGEM_2018_Plate5_Well18J
version: 1
owned by: myers
member: BBa_K1333004

iGEM 2018 Distribution Plate 5 Well 10J
type: Collection
persistent identity: iGEM 2018 Plate5 Well10J
display id: iGEM_2018_Plate5_Well10J
version: 1
owned by: myers
member: BBa_K1323015

iGEM 2018 Distribution Plate 5 Well 2A
type: Collection
persistent identity: iGEM 2018 Plate5 Well2A
display id: iGEM_2018_Plate5_Well2A
version: 1
owned by: myers
member: N-carrier

iGEM 2018 Distribution Plate 5 Well 24D
type: Collection
persistent identity: iGEM 2018 Plate5 Well24D
display id: iGEM_2018_Plate5_Well24D
version: 1
owned by: myers
member: NeoR

iGEM 2018 Distribution Plate 5 Well 11H
type: Collection
persistent identity: iGEM 2018 Plate5 Well11H
display id: iGEM_2018_Plate5_Well11H
version: 1
owned by: myers
member: ONBY RS

iGEM 2018 Distribution Plate 5 Well 23I
type: Collection
persistent identity: iGEM 2018 Plate5 Well23I
display id: iGEM_2018_Plate5_Well23I
version: 1
owned by: myers
member: BBa_K1362051

iGEM 2018 Distribution Plate 5 Well 20M
type: Collection
persistent identity: iGEM 2018 Plate5 Well20M
display id: iGEM_2018_Plate5_Well20M
version: 1
owned by: myers
member: BBa_K1321357

iGEM 2018 Distribution Plate 5 Well 12P
type: Collection
persistent identity: iGEM 2018 Plate5 Well12P
display id: iGEM_2018_Plate5_Well12P
version: 1
owned by: myers
member: BBa_K1418041

iGEM 2018 Distribution Plate 5 Well 11A
type: Collection
persistent identity: iGEM 2018 Plate5 Well11A
display id: iGEM_2018_Plate5_Well11A
version: 1
owned by: myers
member: BBa_K1319042

iGEM 2018 Distribution Plate 5 Well 14N
type: Collection
persistent identity: iGEM 2018 Plate5 Well14N
display id: iGEM_2018_Plate5_Well14N
version: 1
owned by: myers
member: BBa_K1321101

iGEM 2018 Distribution Plate 5 Well 16J
type: Collection
persistent identity: iGEM 2018 Plate5 Well16J
display id: iGEM_2018_Plate5_Well16J
version: 1
owned by: myers
member: BBa_K1321114

iGEM 2018 Distribution Plate 5 Well 20N
type: Collection
persistent identity: iGEM 2018 Plate5 Well20N
display id: iGEM_2018_Plate5_Well20N
version: 1
owned by: myers
member: BBa_K1499400

iGEM 2018 Distribution Plate 5 Well 6M
type: Collection
persistent identity: iGEM 2018 Plate5 Well6M
display id: iGEM_2018_Plate5_Well6M
version: 1
owned by: myers
member: BBa_K1362060

iGEM 2018 Distribution Plate 5 Well 14M
type: Collection
persistent identity: iGEM 2018 Plate5 Well14M
display id: iGEM_2018_Plate5_Well14M
version: 1
owned by: myers
member: BBa_K1497032

iGEM 2018 Distribution Plate 5 Well 12F
type: Collection
persistent identity: iGEM 2018 Plate5 Well12F
display id: iGEM_2018_Plate5_Well12F
version: 1
owned by: myers
member: BBa_K1365120

iGEM 2018 Distribution Plate 5 Well 8M
type: Collection
persistent identity: iGEM 2018 Plate5 Well8M
display id: iGEM_2018_Plate5_Well8M
version: 1
owned by: myers
member: SspDnaB(N)

iGEM 2018 Distribution Plate 5 Well 12C
type: Collection
persistent identity: iGEM 2018 Plate5 Well12C
display id: iGEM_2018_Plate5_Well12C
version: 1
owned by: myers
member: BBa_K1315011

iGEM 2018 Distribution Plate 5 Well 2J
type: Collection
persistent identity: iGEM 2018 Plate5 Well2J
display id: iGEM_2018_Plate5_Well2J
version: 1
owned by: myers
member: BBa_K1351006

iGEM 2018 Distribution Plate 5 Well 18A
type: Collection
persistent identity: iGEM 2018 Plate5 Well18A
display id: iGEM_2018_Plate5_Well18A
version: 1
owned by: myers
member: BBa_K1321340

iGEM 2018 Distribution Plate 5 Well 12I
type: Collection
persistent identity: iGEM 2018 Plate5 Well12I
display id: iGEM_2018_Plate5_Well12I
version: 1
owned by: myers
member: BBa_K1497009

iGEM 2018 Distribution Plate 5 Well 23B
type: Collection
persistent identity: iGEM 2018 Plate5 Well23B
display id: iGEM_2018_Plate5_Well23B
version: 1
owned by: myers
member: mRdRP

iGEM 2018 Distribution Plate 5 Well 4N
type: Collection
persistent identity: iGEM 2018 Plate5 Well4N
display id: iGEM_2018_Plate5_Well4N
version: 1
owned by: myers
member: BBa_K1351030

iGEM 2018 Distribution Plate 5 Well 20L
type: Collection
persistent identity: iGEM 2018 Plate5 Well20L
display id: iGEM_2018_Plate5_Well20L
version: 1
owned by: myers
member: BBa_K1450012

iGEM 2018 Distribution Plate 5 Well 17M
type: Collection
persistent identity: iGEM 2018 Plate5 Well17M
display id: iGEM_2018_Plate5_Well17M
version: 1
owned by: myers
member: PfrI

iGEM 2018 Distribution Plate 5 Well 21L
type: Collection
persistent identity: iGEM 2018 Plate5 Well21L
display id: iGEM_2018_Plate5_Well21L
version: 1
owned by: myers
member: BBa_K1355004

iGEM 2018 Distribution Plate 5 Well 6N
type: Collection
persistent identity: iGEM 2018 Plate5 Well6N
display id: iGEM_2018_Plate5_Well6N
version: 1
owned by: myers
member: norC

iGEM 2018 Distribution Plate 5 Well 17D
type: Collection
persistent identity: iGEM 2018 Plate5 Well17D
display id: iGEM_2018_Plate5_Well17D
version: 1
owned by: myers
member: BFRM52H

iGEM 2018 Distribution Plate 5 Well 21G
type: Collection
persistent identity: iGEM 2018 Plate5 Well21G
display id: iGEM_2018_Plate5_Well21G
version: 1
owned by: myers
member: BBa_K1493510

iGEM 2018 Distribution Plate 5 Well 12B
type: Collection
persistent identity: iGEM 2018 Plate5 Well12B
display id: iGEM_2018_Plate5_Well12B
version: 1
owned by: myers
member: BBa_K1481003

iGEM 2018 Distribution Plate 5 Well 9B
type: Collection
persistent identity: iGEM 2018 Plate5 Well9B
display id: iGEM_2018_Plate5_Well9B
version: 1
owned by: myers
member: BBa_K1399010

iGEM 2018 Distribution Plate 5 Well 22A
type: Collection
persistent identity: iGEM 2018 Plate5 Well22A
display id: iGEM_2018_Plate5_Well22A
version: 1
owned by: myers
member: BBa_K1321333

iGEM 2018 Distribution Plate 5 Well 2N
type: Collection
persistent identity: iGEM 2018 Plate5 Well2N
display id: iGEM_2018_Plate5_Well2N
version: 1
owned by: myers
member: BBa_K1351008

iGEM 2018 Distribution Plate 5 Well 4H
type: Collection
persistent identity: iGEM 2018 Plate5 Well4H
display id: iGEM_2018_Plate5_Well4H
version: 1
owned by: myers
member: BBa_K1351017

iGEM 2018 Distribution Plate 5 Well 3J
type: Collection
persistent identity: iGEM 2018 Plate5 Well3J
display id: iGEM_2018_Plate5_Well3J
version: 1
owned by: myers
member: BBa_K1486022

iGEM 2018 Distribution Plate 5 Well 3D
type: Collection
persistent identity: iGEM 2018 Plate5 Well3D
display id: iGEM_2018_Plate5_Well3D
version: 1
owned by: myers
member: BBa_K1499202

iGEM 2018 Distribution Plate 5 Well 1N
type: Collection
persistent identity: iGEM 2018 Plate5 Well1N
display id: iGEM_2018_Plate5_Well1N
version: 1
owned by: myers
member: BBa_K1491018

iGEM 2018 Distribution Plate 5 Well 17L
type: Collection
persistent identity: iGEM 2018 Plate5 Well17L
display id: iGEM_2018_Plate5_Well17L
version: 1
owned by: myers
member: BBa_K1472610

iGEM 2018 Distribution Plate 5 Well 10G
type: Collection
persistent identity: iGEM 2018 Plate5 Well10G
display id: iGEM_2018_Plate5_Well10G
version: 1
owned by: myers
member: BBa_K1315005

iGEM 2018 Distribution Plate 5 Well 22O
type: Collection
persistent identity: iGEM 2018 Plate5 Well22O
display id: iGEM_2018_Plate5_Well22O
version: 1
owned by: myers
member: BBa_K1321152

iGEM 2018 Distribution Plate 5 Well 1L
type: Collection
persistent identity: iGEM 2018 Plate5 Well1L
display id: iGEM_2018_Plate5_Well1L
version: 1
owned by: myers
member: BBa_K1491017

iGEM 2018 Distribution Plate 5 Well 1P
type: Collection
persistent identity: iGEM 2018 Plate5 Well1P
display id: iGEM_2018_Plate5_Well1P
version: 1
owned by: myers
member: BBa_K1499200

iGEM 2018 Distribution Plate 5 Well 6K
type: Collection
persistent identity: iGEM 2018 Plate5 Well6K
display id: iGEM_2018_Plate5_Well6K
version: 1
owned by: myers
member: BBa_K1362205

iGEM 2018 Distribution Plate 5 Well 19A
type: Collection
persistent identity: iGEM 2018 Plate5 Well19A
display id: iGEM_2018_Plate5_Well19A
version: 1
owned by: myers
member: BBa_K1493601

iGEM 2018 Distribution Plate 5 Well 10P
type: Collection
persistent identity: iGEM 2018 Plate5 Well10P
display id: iGEM_2018_Plate5_Well10P
version: 1
owned by: myers
member: BBa_K1481002

iGEM 2018 Distribution Plate 5 Well 2M
type: Collection
persistent identity: iGEM 2018 Plate5 Well2M
display id: iGEM_2018_Plate5_Well2M
version: 1
owned by: myers
member: BBa_K1362059

iGEM 2018 Distribution Plate 5 Well 24O
type: Collection
persistent identity: iGEM 2018 Plate5 Well24O
display id: iGEM_2018_Plate5_Well24O
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 5 Well 24J
type: Collection
persistent identity: iGEM 2018 Plate5 Well24J
display id: iGEM_2018_Plate5_Well24J
version: 1
owned by: myers
member: BBa_K1375019

iGEM 2018 Distribution Plate 5 Well 2L
type: Collection
persistent identity: iGEM 2018 Plate5 Well2L
display id: iGEM_2018_Plate5_Well2L
version: 1
owned by: myers
member: BBa_K1351007

iGEM 2018 Distribution Plate 5 Well 15E
type: Collection
persistent identity: iGEM 2018 Plate5 Well15E
display id: iGEM_2018_Plate5_Well15E
version: 1
owned by: myers
member: BBa_K1415103

iGEM 2018 Distribution Plate 5 Well 14I
type: Collection
persistent identity: iGEM 2018 Plate5 Well14I
display id: iGEM_2018_Plate5_Well14I
version: 1
owned by: myers
member: BBa_K1497030

iGEM 2018 Distribution Plate 5 Well 3H
type: Collection
persistent identity: iGEM 2018 Plate5 Well3H
display id: iGEM_2018_Plate5_Well3H
version: 1
owned by: myers
member: BBa_K1499000

iGEM 2018 Distribution Plate 5 Well 4C
type: Collection
persistent identity: iGEM 2018 Plate5 Well4C
display id: iGEM_2018_Plate5_Well4C
version: 1
owned by: myers
member: 1NpuE(N*)

iGEM 2018 Distribution Plate 5 Well 17F
type: Collection
persistent identity: iGEM 2018 Plate5 Well17F
display id: iGEM_2018_Plate5_Well17F
version: 1
owned by: myers
member: BBa_K1438002

iGEM 2018 Distribution Plate 5 Well 22B
type: Collection
persistent identity: iGEM 2018 Plate5 Well22B
display id: iGEM_2018_Plate5_Well22B
version: 1
owned by: myers
member: BBa_K1499402

iGEM 2018 Distribution Plate 5 Well 14B
type: Collection
persistent identity: iGEM 2018 Plate5 Well14B
display id: iGEM_2018_Plate5_Well14B
version: 1
owned by: myers
member: BBa_K1499004

iGEM 2018 Distribution Plate 5 Well 4A
type: Collection
persistent identity: iGEM 2018 Plate5 Well4A
display id: iGEM_2018_Plate5_Well4A
version: 1
owned by: myers
member: 1GateNpuE

iGEM 2018 Distribution Plate 5 Well 9D
type: Collection
persistent identity: iGEM 2018 Plate5 Well9D
display id: iGEM_2018_Plate5_Well9D
version: 1
owned by: myers
member: BBa_K1399011

iGEM 2018 Distribution Plate 5 Well 23H
type: Collection
persistent identity: iGEM 2018 Plate5 Well23H
display id: iGEM_2018_Plate5_Well23H
version: 1
owned by: myers
member: BBa_K1351021

iGEM 2018 Distribution Plate 5 Well 6E
type: Collection
persistent identity: iGEM 2018 Plate5 Well6E
display id: iGEM_2018_Plate5_Well6E
version: 1
owned by: myers
member: BBa_K1362173

iGEM 2018 Distribution Plate 5 Well 8P
type: Collection
persistent identity: iGEM 2018 Plate5 Well8P
display id: iGEM_2018_Plate5_Well8P
version: 1
owned by: myers
member: BBa_K1323010

iGEM 2018 Distribution Plate 5 Well 5H
type: Collection
persistent identity: iGEM 2018 Plate5 Well5H
display id: iGEM_2018_Plate5_Well5H
version: 1
owned by: myers
member: BBa_K1467204

iGEM 2018 Distribution Plate 5 Well 16L
type: Collection
persistent identity: iGEM 2018 Plate5 Well16L
display id: iGEM_2018_Plate5_Well16L
version: 1
owned by: myers
member: BBa_K1321115

iGEM 2018 Distribution Plate 5 Well 5J
type: Collection
persistent identity: iGEM 2018 Plate5 Well5J
display id: iGEM_2018_Plate5_Well5J
version: 1
owned by: myers
member: BBa_K1423006

iGEM 2018 Distribution Plate 5 Well 14K
type: Collection
persistent identity: iGEM 2018 Plate5 Well14K
display id: iGEM_2018_Plate5_Well14K
version: 1
owned by: myers
member: BBa_K1497031

iGEM 2018 Distribution Plate 5 Well 6I
type: Collection
persistent identity: iGEM 2018 Plate5 Well6I
display id: iGEM_2018_Plate5_Well6I
version: 1
owned by: myers
member: BBa_K1362204

iGEM 2018 Distribution Plate 5 Well 14F
type: Collection
persistent identity: iGEM 2018 Plate5 Well14F
display id: iGEM_2018_Plate5_Well14F
version: 1
owned by: myers
member: BBa_K1429002

iGEM 2018 Distribution Plate 5 Well 15G
type: Collection
persistent identity: iGEM 2018 Plate5 Well15G
display id: iGEM_2018_Plate5_Well15G
version: 1
owned by: myers
member: BBa_K1415104

iGEM 2018 Distribution Plate 5 Well 2O
type: Collection
persistent identity: iGEM 2018 Plate5 Well2O
display id: iGEM_2018_Plate5_Well2O
version: 1
owned by: myers
member: 1Gate-NpuD

iGEM 2018 Distribution Plate 5 Well 8C
type: Collection
persistent identity: iGEM 2018 Plate5 Well8C
display id: iGEM_2018_Plate5_Well8C
version: 1
owned by: myers
member: SspDnaB(N*

iGEM 2018 Distribution Plate 5 Well 20P
type: Collection
persistent identity: iGEM 2018 Plate5 Well20P
display id: iGEM_2018_Plate5_Well20P
version: 1
owned by: myers
member: BBa_K1499401

iGEM 2018 Distribution Plate 5 Well 3C
type: Collection
persistent identity: iGEM 2018 Plate5 Well3C
display id: iGEM_2018_Plate5_Well3C
version: 1
owned by: myers
member: BBa_K1333106

iGEM 2018 Distribution Plate 5 Well 21C
type: Collection
persistent identity: iGEM 2018 Plate5 Well21C
display id: iGEM_2018_Plate5_Well21C
version: 1
owned by: myers
member: BBa_K1493504

iGEM 2018 Distribution Plate 5 Well 15A
type: Collection
persistent identity: iGEM 2018 Plate5 Well15A
display id: iGEM_2018_Plate5_Well15A
version: 1
owned by: myers
member: BBa_K1415101

iGEM 2018 Distribution Plate 5 Well 1F
type: Collection
persistent identity: iGEM 2018 Plate5 Well1F
display id: iGEM_2018_Plate5_Well1F
version: 1
owned by: myers
member: BBa_K1491009

iGEM 2018 Distribution Plate 5 Well 13C
type: Collection
persistent identity: iGEM 2018 Plate5 Well13C
display id: iGEM_2018_Plate5_Well13C
version: 1
owned by: myers
member: BBa_K1415003

iGEM 2018 Distribution Plate 5 Well 11M
type: Collection
persistent identity: iGEM 2018 Plate5 Well11M
display id: iGEM_2018_Plate5_Well11M
version: 1
owned by: myers
member: BBa_K1330005

iGEM 2018 Distribution Plate 5 Well 18N
type: Collection
persistent identity: iGEM 2018 Plate5 Well18N
display id: iGEM_2018_Plate5_Well18N
version: 1
owned by: myers
member: BBa_K1333321

iGEM 2018 Distribution Plate 5 Well 13D
type: Collection
persistent identity: iGEM 2018 Plate5 Well13D
display id: iGEM_2018_Plate5_Well13D
version: 1
owned by: myers
member: BBa_K1325002

iGEM 2018 Distribution Plate 5 Well 6C
type: Collection
persistent identity: iGEM 2018 Plate5 Well6C
display id: iGEM_2018_Plate5_Well6C
version: 1
owned by: myers
member: BBa_K1362172

iGEM 2018 Distribution Plate 5 Well 6D
type: Collection
persistent identity: iGEM 2018 Plate5 Well6D
display id: iGEM_2018_Plate5_Well6D
version: 1
owned by: myers
member: BBa_K1351033

iGEM 2018 Distribution Plate 5 Well 14J
type: Collection
persistent identity: iGEM 2018 Plate5 Well14J
display id: iGEM_2018_Plate5_Well14J
version: 1
owned by: myers
member: BBa_K1321159

iGEM 2018 Distribution Plate 5 Well 8H
type: Collection
persistent identity: iGEM 2018 Plate5 Well8H
display id: iGEM_2018_Plate5_Well8H
version: 1
owned by: myers
member: BBa_K1456004

iGEM 2018 Distribution Plate 5 Well 10L
type: Collection
persistent identity: iGEM 2018 Plate5 Well10L
display id: iGEM_2018_Plate5_Well10L
version: 1
owned by: myers
member: BBa_K1413021

iGEM 2018 Distribution Plate 5 Well 12N
type: Collection
persistent identity: iGEM 2018 Plate5 Well12N
display id: iGEM_2018_Plate5_Well12N
version: 1
owned by: myers
member: BBa_K1321333

iGEM 2018 Distribution Plate 5 Well 19B
type: Collection
persistent identity: iGEM 2018 Plate5 Well19B
display id: iGEM_2018_Plate5_Well19B
version: 1
owned by: myers
member: BBa_K1349007

iGEM 2018 Distribution Plate 5 Well 7E
type: Collection
persistent identity: iGEM 2018 Plate5 Well7E
display id: iGEM_2018_Plate5_Well7E
version: 1
owned by: myers
member: BBa_K1316012

iGEM 2018 Distribution Plate 5 Well 8A
type: Collection
persistent identity: iGEM 2018 Plate5 Well8A
display id: iGEM_2018_Plate5_Well8A
version: 1
owned by: myers
member: sfGFP_T65C

iGEM 2018 Distribution Plate 5 Well 15H
type: Collection
persistent identity: iGEM 2018 Plate5 Well15H
display id: iGEM_2018_Plate5_Well15H
version: 1
owned by: myers
member: BBa_K1460004

iGEM 2018 Distribution Plate 5 Well 4G
type: Collection
persistent identity: iGEM 2018 Plate5 Well4G
display id: iGEM_2018_Plate5_Well4G
version: 1
owned by: myers
member: SspDnaX(N)

iGEM 2018 Distribution Plate 5 Well 18B
type: Collection
persistent identity: iGEM 2018 Plate5 Well18B
display id: iGEM_2018_Plate5_Well18B
version: 1
owned by: myers
member: BBa_K1508002

iGEM 2018 Distribution Plate 5 Well 19H
type: Collection
persistent identity: iGEM 2018 Plate5 Well19H
display id: iGEM_2018_Plate5_Well19H
version: 1
owned by: myers
member: BBa_K1349000

iGEM 2018 Distribution Plate 5 Well 24P
type: Collection
persistent identity: iGEM 2018 Plate5 Well24P
display id: iGEM_2018_Plate5_Well24P
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 5 Well 13A
type: Collection
persistent identity: iGEM 2018 Plate5 Well13A
display id: iGEM_2018_Plate5_Well13A
version: 1
owned by: myers
member: BBa_K1415002

iGEM 2018 Distribution Plate 5 Well 12D
type: Collection
persistent identity: iGEM 2018 Plate5 Well12D
display id: iGEM_2018_Plate5_Well12D
version: 1
owned by: myers
member: BBa_K1481004

iGEM 2018 Distribution Plate 5 Well 10M
type: Collection
persistent identity: iGEM 2018 Plate5 Well10M
display id: iGEM_2018_Plate5_Well10M
version: 1
owned by: myers
member: BBa_K1315008

iGEM 2018 Distribution Plate 5 Well 4P
type: Collection
persistent identity: iGEM 2018 Plate5 Well4P
display id: iGEM_2018_Plate5_Well4P
version: 1
owned by: myers
member: BBa_K1351031

iGEM 2018 Distribution Plate 5 Well 15C
type: Collection
persistent identity: iGEM 2018 Plate5 Well15C
display id: iGEM_2018_Plate5_Well15C
version: 1
owned by: myers
member: BBa_K1415102

iGEM 2018 Distribution Plate 5 Well 1J
type: Collection
persistent identity: iGEM 2018 Plate5 Well1J
display id: iGEM_2018_Plate5_Well1J
version: 1
owned by: myers
member: BBa_K1491016

iGEM 2018 Distribution Plate 5 Well 8E
type: Collection
persistent identity: iGEM 2018 Plate5 Well8E
display id: iGEM_2018_Plate5_Well8E
version: 1
owned by: myers
member: SspDnaB(C)

iGEM 2018 Distribution Plate 5 Well 11O
type: Collection
persistent identity: iGEM 2018 Plate5 Well11O
display id: iGEM_2018_Plate5_Well11O
version: 1
owned by: myers
member: BBa_K1415001

iGEM 2018 Distribution Plate 5 Well 4E
type: Collection
persistent identity: iGEM 2018 Plate5 Well4E
display id: iGEM_2018_Plate5_Well4E
version: 1
owned by: myers
member: 1NpuE(C*)

iGEM 2018 Distribution Plate 5 Well 16K
type: Collection
persistent identity: iGEM 2018 Plate5 Well16K
display id: iGEM_2018_Plate5_Well16K
version: 1
owned by: myers
member: BBa_K1321337

iGEM 2018 Distribution Plate 5 Well 13F
type: Collection
persistent identity: iGEM 2018 Plate5 Well13F
display id: iGEM_2018_Plate5_Well13F
version: 1
owned by: myers
member: BBa_K1325005

iGEM 2018 Distribution Plate 5 Well 19D
type: Collection
persistent identity: iGEM 2018 Plate5 Well19D
display id: iGEM_2018_Plate5_Well19D
version: 1
owned by: myers
member: BBa_K1349008

iGEM 2018 Distribution Plate 5 Well 23E
type: Collection
persistent identity: iGEM 2018 Plate5 Well23E
display id: iGEM_2018_Plate5_Well23E
version: 1
owned by: myers
member: BBa_K1362023

iGEM 2018 Distribution Plate 5 Well 22M
type: Collection
persistent identity: iGEM 2018 Plate5 Well22M
display id: iGEM_2018_Plate5_Well22M
version: 1
owned by: myers
member: BBa_K1321151

iGEM 2018 Distribution Plate 5 Well 6B
type: Collection
persistent identity: iGEM 2018 Plate5 Well6B
display id: iGEM_2018_Plate5_Well6B
version: 1
owned by: myers
member: BBa_K1351032

iGEM 2018 Distribution Plate 5 Well 18K
type: Collection
persistent identity: iGEM 2018 Plate5 Well18K
display id: iGEM_2018_Plate5_Well18K
version: 1
owned by: myers
member: BBa_K1321345

iGEM 2018 Distribution Plate 5 Well 1C
type: Collection
persistent identity: iGEM 2018 Plate5 Well1C
display id: iGEM_2018_Plate5_Well1C
version: 1
owned by: myers
member: BBa_K1404002

iGEM 2018 Distribution Plate 5 Well 14E
type: Collection
persistent identity: iGEM 2018 Plate5 Well14E
display id: iGEM_2018_Plate5_Well14E
version: 1
owned by: myers
member: BBa_K1497028

iGEM 2018 Distribution Plate 5 Well 5K
type: Collection
persistent identity: iGEM 2018 Plate5 Well5K
display id: iGEM_2018_Plate5_Well5K
version: 1
owned by: myers
member: BBa_K1333309

iGEM 2018 Distribution Plate 5 Well 22K
type: Collection
persistent identity: iGEM 2018 Plate5 Well22K
display id: iGEM_2018_Plate5_Well22K
version: 1
owned by: myers
member: BBa_K1321138

iGEM 2018 Distribution Plate 5 Well 16I
type: Collection
persistent identity: iGEM 2018 Plate5 Well16I
display id: iGEM_2018_Plate5_Well16I
version: 1
owned by: myers
member: BBa_K1321009

iGEM 2018 Distribution Plate 5 Well 19M
type: Collection
persistent identity: iGEM 2018 Plate5 Well19M
display id: iGEM_2018_Plate5_Well19M
version: 1
owned by: myers
member: BBa_K1493806

iGEM 2018 Distribution Plate 5 Well 8B
type: Collection
persistent identity: iGEM 2018 Plate5 Well8B
display id: iGEM_2018_Plate5_Well8B
version: 1
owned by: myers
member: BBa_K1510003

iGEM 2018 Distribution Plate 5 Well 1E
type: Collection
persistent identity: iGEM 2018 Plate5 Well1E
display id: iGEM_2018_Plate5_Well1E
version: 1
owned by: myers
member: BBa_K1404006

iGEM 2018 Distribution Plate 5 Well 24I
type: Collection
persistent identity: iGEM 2018 Plate5 Well24I
display id: iGEM_2018_Plate5_Well24I
version: 1
owned by: myers
member: BBa_K1321163

iGEM 2018 Distribution Plate 5 Well 20J
type: Collection
persistent identity: iGEM 2018 Plate5 Well20J
display id: iGEM_2018_Plate5_Well20J
version: 1
owned by: myers
member: BBa_K1450004

iGEM 2018 Distribution Plate 5 Well 17G
type: Collection
persistent identity: iGEM 2018 Plate5 Well17G
display id: iGEM_2018_Plate5_Well17G
version: 1
owned by: myers
member: Ag43 RCF10

iGEM 2018 Distribution Plate 5 Well 13G
type: Collection
persistent identity: iGEM 2018 Plate5 Well13G
display id: iGEM_2018_Plate5_Well13G
version: 1
owned by: myers
member: BBa_K1415005

iGEM 2018 Distribution Plate 5 Well 3G
type: Collection
persistent identity: iGEM 2018 Plate5 Well3G
display id: iGEM_2018_Plate5_Well3G
version: 1
owned by: myers
member: BBa_K1333201

iGEM 2018 Distribution Plate 5 Well 20G
type: Collection
persistent identity: iGEM 2018 Plate5 Well20G
display id: iGEM_2018_Plate5_Well20G
version: 1
owned by: myers
member: BBa_K1321352

iGEM 2018 Distribution Plate 5 Well 24L
type: Collection
persistent identity: iGEM 2018 Plate5 Well24L
display id: iGEM_2018_Plate5_Well24L
version: 1
owned by: myers
member: BBa_K1375020

iGEM 2018 Distribution Plate 5 Well 22C
type: Collection
persistent identity: iGEM 2018 Plate5 Well22C
display id: iGEM_2018_Plate5_Well22C
version: 1
owned by: myers
member: BBa_K1321120

iGEM 2018 Distribution Plate 5 Well 5L
type: Collection
persistent identity: iGEM 2018 Plate5 Well5L
display id: iGEM_2018_Plate5_Well5L
version: 1
owned by: myers
member: BBa_K1365000

iGEM 2018 Distribution Plate 5 Well 22L
type: Collection
persistent identity: iGEM 2018 Plate5 Well22L
display id: iGEM_2018_Plate5_Well22L
version: 1
owned by: myers
member: BBa_K1401601

iGEM 2018 Distribution Plate 5 Well 4M
type: Collection
persistent identity: iGEM 2018 Plate5 Well4M
display id: iGEM_2018_Plate5_Well4M
version: 1
owned by: myers
member: SspGyrB(C)

iGEM 2018 Distribution Plate 5 Well 11J
type: Collection
persistent identity: iGEM 2018 Plate5 Well11J
display id: iGEM_2018_Plate5_Well11J
version: 1
owned by: myers
member: BBa_K1334002

iGEM 2018 Distribution Plate 5 Well 2G
type: Collection
persistent identity: iGEM 2018 Plate5 Well2G
display id: iGEM_2018_Plate5_Well2G
version: 1
owned by: myers
member: BBa_K1362056

iGEM 2018 Distribution Plate 5 Well 10O
type: Collection
persistent identity: iGEM 2018 Plate5 Well10O
display id: iGEM_2018_Plate5_Well10O
version: 1
owned by: myers
member: BBa_K1315009

iGEM 2018 Distribution Plate 5 Well 16M
type: Collection
persistent identity: iGEM 2018 Plate5 Well16M
display id: iGEM_2018_Plate5_Well16M
version: 1
owned by: myers
member: BBa_K1321338

iGEM 2018 Distribution Plate 5 Well 9N
type: Collection
persistent identity: iGEM 2018 Plate5 Well9N
display id: iGEM_2018_Plate5_Well9N
version: 1
owned by: myers
member: BBa_K1331004

iGEM 2018 Distribution Plate 5 Well 23D
type: Collection
persistent identity: iGEM 2018 Plate5 Well23D
display id: iGEM_2018_Plate5_Well23D
version: 1
owned by: myers
member: C2HP/RGE

iGEM 2018 Distribution Plate 5 Well 5I
type: Collection
persistent identity: iGEM 2018 Plate5 Well5I
display id: iGEM_2018_Plate5_Well5I
version: 1
owned by: myers
member: BBa_K1333308

iGEM 2018 Distribution Plate 5 Well 22F
type: Collection
persistent identity: iGEM 2018 Plate5 Well22F
display id: iGEM_2018_Plate5_Well22F
version: 1
owned by: myers
member: BBa_K1505000

iGEM 2018 Distribution Plate 5 Well 14L
type: Collection
persistent identity: iGEM 2018 Plate5 Well14L
display id: iGEM_2018_Plate5_Well14L
version: 1
owned by: myers
member: BBa_K1321162

iGEM 2018 Distribution Plate 5 Well 17P
type: Collection
persistent identity: iGEM 2018 Plate5 Well17P
display id: iGEM_2018_Plate5_Well17P
version: 1
owned by: myers
member: BBa_K1523022

iGEM 2018 Distribution Plate 5 Well 13E
type: Collection
persistent identity: iGEM 2018 Plate5 Well13E
display id: iGEM_2018_Plate5_Well13E
version: 1
owned by: myers
member: BBa_K1415004

iGEM 2018 Distribution Plate 5 Well 11F
type: Collection
persistent identity: iGEM 2018 Plate5 Well11F
display id: iGEM_2018_Plate5_Well11F
version: 1
owned by: myers
member: BBa_K1343014

iGEM 2018 Distribution Plate 5 Well 16C
type: Collection
persistent identity: iGEM 2018 Plate5 Well16C
display id: iGEM_2018_Plate5_Well16C
version: 1
owned by: myers
member: BBa_K1321094

iGEM 2018 Distribution Plate 5 Well 11K
type: Collection
persistent identity: iGEM 2018 Plate5 Well11K
display id: iGEM_2018_Plate5_Well11K
version: 1
owned by: myers
member: BBa_K1330004

iGEM 2018 Distribution Plate 5 Well 16O
type: Collection
persistent identity: iGEM 2018 Plate5 Well16O
display id: iGEM_2018_Plate5_Well16O
version: 1
owned by: myers
member: BBa_K1321339

iGEM 2018 Distribution Plate 5 Well 22I
type: Collection
persistent identity: iGEM 2018 Plate5 Well22I
display id: iGEM_2018_Plate5_Well22I
version: 1
owned by: myers
member: BBa_K1321125

iGEM 2018 Distribution Plate 5 Well 11G
type: Collection
persistent identity: iGEM 2018 Plate5 Well11G
display id: iGEM_2018_Plate5_Well11G
version: 1
owned by: myers
member: BBa_K1330002

iGEM 2018 Distribution Plate 5 Well 18I
type: Collection
persistent identity: iGEM 2018 Plate5 Well18I
display id: iGEM_2018_Plate5_Well18I
version: 1
owned by: myers
member: BBa_K1321344

iGEM 2018 Distribution Plate 5 Well 14G
type: Collection
persistent identity: iGEM 2018 Plate5 Well14G
display id: iGEM_2018_Plate5_Well14G
version: 1
owned by: myers
member: BBa_K1497029

iGEM 2018 Distribution Plate 5 Well 11D
type: Collection
persistent identity: iGEM 2018 Plate5 Well11D
display id: iGEM_2018_Plate5_Well11D
version: 1
owned by: myers
member: BBa_K1509000

iGEM 2018 Distribution Plate 5 Well 2B
type: Collection
persistent identity: iGEM 2018 Plate5 Well2B
display id: iGEM_2018_Plate5_Well2B
version: 1
owned by: myers
member: BBa_K1351002

iGEM 2018 Distribution Plate 5 Well 20I
type: Collection
persistent identity: iGEM 2018 Plate5 Well20I
display id: iGEM_2018_Plate5_Well20I
version: 1
owned by: myers
member: BBa_K1321353

iGEM 2018 Distribution Plate 5 Well 23P
type: Collection
persistent identity: iGEM 2018 Plate5 Well23P
display id: iGEM_2018_Plate5_Well23P
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 5 Well 4J
type: Collection
persistent identity: iGEM 2018 Plate5 Well4J
display id: iGEM_2018_Plate5_Well4J
version: 1
owned by: myers
member: BBa_K1351028

iGEM 2018 Distribution Plate 5 Well 4K
type: Collection
persistent identity: iGEM 2018 Plate5 Well4K
display id: iGEM_2018_Plate5_Well4K
version: 1
owned by: myers
member: SspGyrB(N)

iGEM 2018 Distribution Plate 5 Well 22P
type: Collection
persistent identity: iGEM 2018 Plate5 Well22P
display id: iGEM_2018_Plate5_Well22P
version: 1
owned by: myers
member: ComE

iGEM 2018 Distribution Plate 5 Well 15M
type: Collection
persistent identity: iGEM 2018 Plate5 Well15M
display id: iGEM_2018_Plate5_Well15M
version: 1
owned by: myers
member: BBa_K1415107

iGEM 2018 Distribution Plate 5 Well 4I
type: Collection
persistent identity: iGEM 2018 Plate5 Well4I
display id: iGEM_2018_Plate5_Well4I
version: 1
owned by: myers
member: BBa_K1362111

iGEM 2018 Distribution Plate 5 Well 3L
type: Collection
persistent identity: iGEM 2018 Plate5 Well3L
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iGEM 2018 Distribution Plate 5 Well 5M
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version: 1
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iGEM 2018 Distribution Plate 5 Well 17I
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iGEM 2018 Distribution Plate 5 Well 13K
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iGEM 2018 Distribution Plate 5 Well 17K
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iGEM 2018 Distribution Plate 5 Well 19J
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iGEM 2018 Distribution Plate 5 Well 13P
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iGEM 2018 Distribution Plate 5 Well 3I
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iGEM 2018 Distribution Plate 5 Well 8J
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iGEM 2018 Distribution Plate 5 Well 6F
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iGEM 2018 Distribution Plate 5 Well 10B
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iGEM 2018 Distribution Plate 5 Well 24A
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iGEM 2018 Distribution Plate 5 Well 7D
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iGEM 2018 Distribution Plate 5 Well 17N
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iGEM 2018 Distribution Plate 5 Well 21P
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iGEM 2018 Distribution Plate 5 Well 11B
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iGEM 2018 Distribution Plate 5 Well 19K
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iGEM 2018 Distribution Plate 5 Well 4O
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iGEM 2018 Distribution Plate 5 Well 15K
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iGEM 2018 Distribution Plate 5 Well 4B
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iGEM 2018 Distribution Plate 5 Well 5G
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iGEM 2018 Distribution Plate 5 Well 5O
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iGEM 2018 Distribution Plate 5 Well 9H
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iGEM 2018 Distribution Plate 5 Well 14P
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iGEM 2018 Distribution Plate 5 Well 3E
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iGEM 2018 Distribution Plate 5 Well 4L
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iGEM 2018 Distribution Plate 5 Well 2K
type: Collection
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iGEM 2018 Distribution Plate 5 Well 3B
type: Collection
persistent identity: iGEM 2018 Plate5 Well3B
display id: iGEM_2018_Plate5_Well3B
version: 1
owned by: myers
member: BBa_K1499201

BBa_K1321114
type: ComponentDefinition
persistent identity: BBa K1321114
display id: BBa_K1321114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to phytochelatin (PC) EC20 with LacI promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD4+PhytoC with LacI
mutable notes: CBD4+PhytoC with LacI
mutable provenance: CBD4+PhytoC with LacI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321002
component: BBa_J04500
component: BBa_K1319106
component: BBa_K1321005
sequence annotation: BBa_J04500
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1319106
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321114_sequence/1

BBa_B0105
type: Component
persistent identity: component2414934
display id: component2414934
version: 1
top level: BBa_K1321114
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: Component
persistent identity: component2414932
display id: component2414932
version: 1
top level: BBa_K1321114
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_J04500
type: Component
persistent identity: component2414929
display id: component2414929
version: 1
top level: BBa_K1321114
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1319106
type: Component
persistent identity: component2414931
display id: component2414931
version: 1
top level: BBa_K1321114
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321005
type: Component
persistent identity: component2414936
display id: component2414936
version: 1
top level: BBa_K1321114
access: public
definition: BBa_K1321005

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414929
display id: annotation2414929
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414929/range2414929/1
component: BBa_J04500

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type: Range
persistent identity: range2414929
display id: range2414929
version: 1
direction: direction/reverse
top level: BBa_K1321114
start: 1
end: 220
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414936
display id: annotation2414936
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414936/range2414936/1
component: BBa_K1321005

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type: Range
persistent identity: range2414936
display id: range2414936
version: 1
direction: direction/reverse
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start: 542
end: 664
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414931
display id: annotation2414931
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414931/range2414931/1
component: BBa_K1319106

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type: Range
persistent identity: range2414931
display id: range2414931
version: 1
direction: direction/reverse
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start: 221
end: 235
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414934
display id: annotation2414934
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414934/range2414934/1
component: BBa_B0105

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type: Range
persistent identity: range2414934
display id: range2414934
version: 1
direction: direction/reverse
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start: 536
end: 541
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414932
display id: annotation2414932
version: 1
top level: BBa_K1321114
location: https://synbiohub.org/public/igem/BBa_K1321114/annotation2414932/range2414932/1
component: BBa_K1321002

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type: Range
persistent identity: range2414932
display id: range2414932
version: 1
direction: direction/reverse
top level: BBa_K1321114
start: 236
end: 535
orientation: inline

BBa_K1415105
type: ComponentDefinition
persistent identity: BBa K1415105
display id: BBa_K1415105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons+B0034+PBAN(Spodoptera litura)
creator: HO, TSUNG YU
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part has a constitutive promoter, so our PBAN(Spodoptera litura) can constitutive express.
mutable notes: No
mutable provenance: Assemble
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1415005
component: BBa_J23101
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J23101
sequence annotation: BBa_K1415005
sequence: https://synbiohub.org/public/igem/BBa_K1415105_sequence/1

BBa_K1415005
type: Component
persistent identity: component2393979
display id: component2393979
version: 1
top level: BBa_K1415105
access: public
definition: BBa_K1415005

BBa_J23101
type: Component
persistent identity: component2393975
display id: component2393975
version: 1
top level: BBa_K1415105
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_B0034
type: Component
persistent identity: component2393977
display id: component2393977
version: 1
top level: BBa_K1415105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393977
display id: annotation2393977
version: 1
top level: BBa_K1415105
location: https://synbiohub.org/public/igem/BBa_K1415105/annotation2393977/range2393977/1
component: BBa_B0034

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persistent identity: range2393977
display id: range2393977
version: 1
direction: direction/reverse
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start: 44
end: 55
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BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2393975
display id: annotation2393975
version: 1
top level: BBa_K1415105
location: https://synbiohub.org/public/igem/BBa_K1415105/annotation2393975/range2393975/1
component: BBa_J23101

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type: Range
persistent identity: range2393975
display id: range2393975
version: 1
direction: direction/reverse
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start: 1
end: 35
orientation: inline

BBa_K1415005
type: SequenceAnnotation
persistent identity: annotation2393979
display id: annotation2393979
version: 1
top level: BBa_K1415105
location: https://synbiohub.org/public/igem/BBa_K1415105/annotation2393979/range2393979/1
component: BBa_K1415005

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type: Range
persistent identity: range2393979
display id: range2393979
version: 1
direction: direction/reverse
top level: BBa_K1415105
start: 62
end: 170
orientation: inline

BBa_K1319008
type: ComponentDefinition
persistent identity: BBa K1319008
display id: BBa_K1319008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG-induced and T7-driven expression of TEV protease
creator: Michael Osthege, Florian Gohr
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This protein generator produces TEV protease when induced with IPTG in a DE3 strain.
mutable notes: We created K1319004 in RFC[25] to enable potential
mutable provenance: The part is combined from other BioBricks and a codon-optimized TEV protease CDS (K1319004).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1319004
component: BBa_K1319106
component: BBa_B0015
component: BBa_I719005
component: BBa_K1319107
component: BBa_G0002
sequence annotation: BBa_G0002
sequence annotation: BBa_K1319107
sequence annotation: BBa_K1319004
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_I719005
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1319008_sequence/1

BBa_B0034
type: Component
persistent identity: component2393322
display id: component2393322
version: 1
top level: BBa_K1319008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1319004
type: Component
persistent identity: component2393327
display id: component2393327
version: 1
top level: BBa_K1319008
access: public
definition: BBa_K1319004

BBa_K1319106
type: Component
persistent identity: component2393324
display id: component2393324
version: 1
top level: BBa_K1319008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_B0015
type: Component
persistent identity: component2393336
display id: component2393336
version: 1
top level: BBa_K1319008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I719005
type: Component
persistent identity: component2393319
display id: component2393319
version: 1
top level: BBa_K1319008
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_K1319107
type: Component
persistent identity: component2393329
display id: component2393329
version: 1
top level: BBa_K1319008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319107/1

BBa_G0002
type: Component
persistent identity: component2393320
display id: component2393320
version: 1
top level: BBa_K1319008
access: public
definition: https://synbiohub.org/public/igem/BBa_G0002/1

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2393320
display id: annotation2393320
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393320/range2393320/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393320/range2393320/1
type: Range
persistent identity: range2393320
display id: range2393320
version: 1
direction: direction/reverse
top level: BBa_K1319008
start: 24
end: 31
orientation: inline

BBa_K1319107
type: SequenceAnnotation
persistent identity: annotation2393329
display id: annotation2393329
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393329/range2393329/1
component: BBa_K1319107

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393329/range2393329/1
type: Range
persistent identity: range2393329
display id: range2393329
version: 1
direction: direction/reverse
top level: BBa_K1319008
start: 785
end: 801
orientation: inline

BBa_K1319004
type: SequenceAnnotation
persistent identity: annotation2393327
display id: annotation2393327
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393327/range2393327/1
component: BBa_K1319004

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393327/range2393327/1
type: Range
persistent identity: range2393327
display id: range2393327
version: 1
direction: direction/reverse
top level: BBa_K1319008
start: 59
end: 784
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393322
display id: annotation2393322
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393322/range2393322/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393322/range2393322/1
type: Range
persistent identity: range2393322
display id: range2393322
version: 1
direction: direction/reverse
top level: BBa_K1319008
start: 32
end: 43
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2393336
display id: annotation2393336
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393336/range2393336/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393336/range2393336/1
type: Range
persistent identity: range2393336
display id: range2393336
version: 1
direction: direction/reverse
top level: BBa_K1319008
start: 802
end: 930
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2393319
display id: annotation2393319
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393319/range2393319/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393319/range2393319/1
type: Range
persistent identity: range2393319
display id: range2393319
version: 1
direction: direction/reverse
top level: BBa_K1319008
start: 1
end: 23
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2393324
display id: annotation2393324
version: 1
top level: BBa_K1319008
location: https://synbiohub.org/public/igem/BBa_K1319008/annotation2393324/range2393324/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1319008/annotation2393324/range2393324/1
type: Range
persistent identity: range2393324
display id: range2393324
version: 1
direction: direction/reverse
top level: BBa_K1319008
start: 44
end: 58
orientation: inline

BBa_K1529311
type: ComponentDefinition
persistent identity: BBa K1529311
display id: BBa_K1529311
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prhl(LR)_GFP
creator: Kohdai Hibi
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:10:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1911_
m user id: 0
owner id: 22688
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1529310
component: BBa_J54103
sequence annotation: BBa_J54103
sequence annotation: BBa_K1529310
sequence: https://synbiohub.org/public/igem/BBa_K1529311_sequence/1

BBa_K1529310
type: Component
persistent identity: component2417626
display id: component2417626
version: 1
top level: BBa_K1529311
access: public
definition: https://synbiohub.org/public/igem/BBa_K1529310/1

BBa_J54103
type: Component
persistent identity: component2417641
display id: component2417641
version: 1
top level: BBa_K1529311
access: public
definition: https://synbiohub.org/public/igem/BBa_J54103/1

BBa_J54103
type: SequenceAnnotation
persistent identity: annotation2417641
display id: annotation2417641
version: 1
top level: BBa_K1529311
location: https://synbiohub.org/public/igem/BBa_K1529311/annotation2417641/range2417641/1
component: BBa_J54103

https://synbiohub.org/public/igem/BBa_K1529311/annotation2417641/range2417641/1
type: Range
persistent identity: range2417641
display id: range2417641
version: 1
direction: direction/reverse
top level: BBa_K1529311
start: 64
end: 990
orientation: inline

BBa_K1529310
type: SequenceAnnotation
persistent identity: annotation2417626
display id: annotation2417626
version: 1
top level: BBa_K1529311
location: https://synbiohub.org/public/igem/BBa_K1529311/annotation2417626/range2417626/1
component: BBa_K1529310

https://synbiohub.org/public/igem/BBa_K1529311/annotation2417626/range2417626/1
type: Range
persistent identity: range2417626
display id: range2417626
version: 1
direction: direction/reverse
top level: BBa_K1529311
start: 1
end: 55
orientation: inline

BBa_K1331003
type: ComponentDefinition
persistent identity: BBa K1331003
display id: BBa_K1331003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlI Acyl-homoserine-lactone synthase from P.aeruginosa SQ6
creator: Jiayi Dong
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
group u list: _1706_
m user id: 0
owner id: 20167
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This sequece encodes RhlI, an acyl-homoserine-lactone synthase required for the synthesis of BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone) autoinducer molecules which bind to RhlR.
mutable notes: It includes both the complete coding sequence of RhlI and part of noncoding sequence between RhlR and RhlI gene.
mutable provenance: It comes from the genomic sequence of P.aeruginosa SQ6.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RhlI
sequence annotation: noncoding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1331003_sequence/1

RhlI
type: SequenceAnnotation
persistent identity: annotation2427973
display id: annotation2427973
version: 1
top level: BBa_K1331003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331003/annotation2427973/range2427973/1

https://synbiohub.org/public/igem/BBa_K1331003/annotation2427973/range2427973/1
type: Range
persistent identity: range2427973
display id: range2427973
version: 1
direction: direction/forward
top level: BBa_K1331003
start: 114
end: 719
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427981
display id: annotation2427981
version: 1
top level: BBa_K1331003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331003/annotation2427981/range2427981/1

https://synbiohub.org/public/igem/BBa_K1331003/annotation2427981/range2427981/1
type: Range
persistent identity: range2427981
display id: range2427981
version: 1
direction: direction/forward
top level: BBa_K1331003
start: 1
end: 113
orientation: inline

BBa_K1438002
type: ComponentDefinition
persistent identity: BBa K1438002
display id: BBa_K1438002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hu_Ferritin
creator: Johann Bauerfeind
created: 2014-10-05T11:00:00Z
modified: 2015-07-22T02:13:57Z
discontinued: false
dominant: false
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Human ferritins do loads more iron atoms then bacterial ferritins. Furthermore they are forming metall cores that are less contaminated with phosphates then e.g. bacterial ferritins. Due to this fact, this should result in a purer iron crystal with an improved magnetic moment.
mutable notes: This design was provided by iGEM Calgary and the HuFerritin as a single protein coding biobrick was constructed by iGEM Berlin as this biobrick was not accessible in this version in the registry.
mutable provenance: iGEM Calgary 2013 from a synthesized codon optimized construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1438002_sequence/1

BBa_K1351004
type: ComponentDefinition
persistent identity: BBa K1351004
display id: BBa_K1351004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SigD-dependent promoter of the LytABC locus (PlytA) of Bacillus subtilis
creator: Mona Dotzler
created: 2014-10-04T11:00:00Z
modified: 2015-06-01T08:15:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PlytA promoter of the ''B. subtilis'' LytABC operon, under the control of SigD (major), the transcripts being predominants at the exponential growth phase, and SigA (minor).
mutable notes: to be filled
mutable provenance: This part was generated by amplification from ''B. subtilis'' W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3. PlytA_ENX_fwd: TGCAGAATTCGCGGCCGCTTCTAGAG ctcatcctttgcacctcg PlytA_SNP_rev: AGCCTGCAGCGGCCGCTACTAGTA tcattatattttatcgtcaacctatt
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1351004_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2394208
display id: annotation2394208
version: 1
top level: BBa_K1351004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1351004/annotation2394208/range2394208/1

https://synbiohub.org/public/igem/BBa_K1351004/annotation2394208/range2394208/1
type: Range
persistent identity: range2394208
display id: range2394208
version: 1
direction: direction/forward
top level: BBa_K1351004
start: 143
end: 146
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2394209
display id: annotation2394209
version: 1
top level: BBa_K1351004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1351004/annotation2394209/range2394209/1

https://synbiohub.org/public/igem/BBa_K1351004/annotation2394209/range2394209/1
type: Range
persistent identity: range2394209
display id: range2394209
version: 1
direction: direction/forward
top level: BBa_K1351004
start: 161
end: 168
orientation: inline

BBa_K1332001
type: ComponentDefinition
persistent identity: BBa K1332001
display id: BBa_K1332001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Histidine tag (8 AA) and RFP
creator: Kenta Nomura
created: 2014-09-24T11:00:00Z
modified: 2015-09-17T07:39:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1707_
m user id: 20591
owner id: 21011
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part consists of histidine tag and RFP. His-tag is useful for purifying RFP.
mutable notes: None
mutable provenance: BBa_E1010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1332001_sequence/1

BBa_K1331002
type: ComponentDefinition
persistent identity: BBa K1331002
display id: BBa_K1331002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR regulatory protein from P. aeruginosa SQ6
creator: Jiayi Dong
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
group u list: _1706_
m user id: 0
owner id: 20167
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding a transcription regulator RhlR. RhlR is necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. It also functions as a transcriptional activator of elastase structural gene (lasB). RhlR binds to autoinducer molecules BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone). This part includes the noncoding sequence of RhlR.
mutable notes: It includes both the complete coding sequence and part of noncoding sequence of RhlR.
mutable provenance: It comes from the genomic sequence of Pseudomonas aeruginosa SQ6.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RhlR
sequence annotation: noncoding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1331002_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation2386611
display id: annotation2386611
version: 1
top level: BBa_K1331002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331002/annotation2386611/range2386611/1

https://synbiohub.org/public/igem/BBa_K1331002/annotation2386611/range2386611/1
type: Range
persistent identity: range2386611
display id: range2386611
version: 1
direction: direction/forward
top level: BBa_K1331002
start: 104
end: 826
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427979
display id: annotation2427979
version: 1
top level: BBa_K1331002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331002/annotation2427979/range2427979/1

https://synbiohub.org/public/igem/BBa_K1331002/annotation2427979/range2427979/1
type: Range
persistent identity: range2427979
display id: range2427979
version: 1
direction: direction/forward
top level: BBa_K1331002
start: 827
end: 897
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427980
display id: annotation2427980
version: 1
top level: BBa_K1331002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331002/annotation2427980/range2427980/1

https://synbiohub.org/public/igem/BBa_K1331002/annotation2427980/range2427980/1
type: Range
persistent identity: range2427980
display id: range2427980
version: 1
direction: direction/forward
top level: BBa_K1331002
start: 1
end: 103
orientation: inline

BBa_K1343020
type: ComponentDefinition
persistent identity: BBa K1343020
display id: BBa_K1343020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux->Green Renilla Luciferase->lacI
creator: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1718_
m user id: 0
owner id: 20669
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The promoter Plux is induced by the binding of an AHL-luxR dimer. This bio-brick leads to the synthesis of the enzyme Green Renilla Luciferase, which catalyses a luminescent reaction. lacI can be used to repress the promoter Plac.
mutable notes: r
mutable provenance: BBa_R0062: Plux, BBa_B0030: Strong RBS, BBa_B0030: Strong RBS, BBa_B0010:T1 (terminator) from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1343020_sequence/1

BBa_K1429002
type: ComponentDefinition
persistent identity: BBa K1429002
display id: BBa_K1429002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cyan Fluorescent Protein (CFP) "Cindy Lou" coding region, intellectual property-free
creator: Christal Gordon
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1807_
m user id: 0
owner id: 22493
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: IP free cyan fluorescent protein.
mutable notes: We had to make sure that we could do the transformation properly.
mutable provenance: I think that CFP is a variant of GFP.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1429002_sequence/1

NeoR
type: ComponentDefinition
persistent identity: BBa K1313004
display id: BBa_K1313004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Neomycin Resistance
creator: Carlos Meza
created: 2014-10-11T11:00:00Z
modified: 2016-01-28T01:43:08Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1688_
m user id: 4206
owner id: 19941
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Neomycin resistance gene. This gene can be used for selection of cells with an efficient transformation. This gene codes neomycin phosphotransferase which breaks neomycin, kanamycin, and gentamicin molecules. If you use these gene as selectivity gene in bacteria, and your laboratory lacks neomycin, you can replace it for kanamycin by adding 35 mg/ml per liter of LB media. The best concentration of neomycin to use is 80 mg/ml per liter of LB media. This gene can be also used as a selective gene for mammalian cells using gentamicin.
mutable notes: Pst1 site cuts gene.
mutable provenance: This gene was obtained from pCDNA 3.1 myc His(A).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1313004_sequence/1

EABase
type: ComponentDefinition
persistent identity: BBa K1483001
display id: BBa_K1483001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Endo-β-Galactosidase
creator: Philipp H. O. Mayer and Philip Roessler
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1863_
m user id: 0
owner id: 21259
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Encodes for an enzyme, that is capable of cleaving A- and B-antigens in the human ABO-Bloodgroup system. As the name suggests, the galactose and connected saccharide residues are cleaved from the antigen, leaving behind the the Oh-Antigen which is characteristic of the Bombay-blood type. Part in RFC25.
mutable notes: Part in RFC25, in order to ease fusion with other parts, for example immobilisation tags.
mutable provenance: The protein encoded by this sequence naturally occurs in Clostridium perfringens. This sequence was optimized for codon usage in E. coli K12 and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Endo-Beta-Galactosidase
sequence: https://synbiohub.org/public/igem/BBa_K1483001_sequence/1

Endo-Beta-Galactosidase
type: SequenceAnnotation
persistent identity: annotation2396228
display id: annotation2396228
version: 1
top level: EABase
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1483001/annotation2396228/range2396228/1

https://synbiohub.org/public/igem/BBa_K1483001/annotation2396228/range2396228/1
type: Range
persistent identity: range2396228
display id: range2396228
version: 1
direction: direction/forward
top level: EABase
start: 1
end: 2397
orientation: inline

BBa_K1491018
type: ComponentDefinition
persistent identity: BBa K1491018
display id: BBa_K1491018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Inducible Codon Optimized Supernova
creator: Danielle Peters
created: 2014-08-03T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Supernova is the monomeric version of KillerRed (BB_K1184000). Codon optimized Supernova with WTLac promoter allows for induction of Supernova expression with IPTG.
mutable notes: Lac inducible Supernova
mutable provenance: The original KillerRed is BBa_K1184000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1491017
component: BBa_J04500
sequence annotation: BBa_K1491017
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1491018_sequence/1

BBa_K1491017
type: Component
persistent identity: component2380367
display id: component2380367
version: 1
top level: BBa_K1491018
access: public
definition: BBa_K1491017

BBa_J04500
type: Component
persistent identity: component2380365
display id: component2380365
version: 1
top level: BBa_K1491018
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1491017
type: SequenceAnnotation
persistent identity: annotation2380367
display id: annotation2380367
version: 1
top level: BBa_K1491018
location: https://synbiohub.org/public/igem/BBa_K1491018/annotation2380367/range2380367/1
component: BBa_K1491017

https://synbiohub.org/public/igem/BBa_K1491018/annotation2380367/range2380367/1
type: Range
persistent identity: range2380367
display id: range2380367
version: 1
direction: direction/reverse
top level: BBa_K1491018
start: 227
end: 967
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2380365
display id: annotation2380365
version: 1
top level: BBa_K1491018
location: https://synbiohub.org/public/igem/BBa_K1491018/annotation2380365/range2380365/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1491018/annotation2380365/range2380365/1
type: Range
persistent identity: range2380365
display id: range2380365
version: 1
direction: direction/reverse
top level: BBa_K1491018
start: 1
end: 220
orientation: inline

BBa_K1321339
type: ComponentDefinition
persistent identity: BBa K1321339
display id: BBa_K1321339
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcenA+Linker, RFC 25 standard
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:14:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: CBDcenA in Freiburg format (RFC 25).
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker: Pro-Thr box
sequence annotation: CBDCenA
sequence: https://synbiohub.org/public/igem/BBa_K1321339_sequence/1

Linker: Pro-Thr box
type: SequenceAnnotation
persistent identity: annotation2414614
display id: annotation2414614
version: 1
top level: BBa_K1321339
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321339/annotation2414614/range2414614/1

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type: Range
persistent identity: range2414614
display id: range2414614
version: 1
direction: direction/forward
top level: BBa_K1321339
start: 319
end: 408
orientation: inline

CBDCenA
type: SequenceAnnotation
persistent identity: annotation2418065
display id: annotation2418065
version: 1
top level: BBa_K1321339
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321339/annotation2418065/range2418065/1

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type: Range
persistent identity: range2418065
display id: range2418065
version: 1
direction: direction/forward
top level: BBa_K1321339
start: 1
end: 318
orientation: inline

SspGyrB(C)
type: ComponentDefinition
persistent identity: BBa K1362121
display id: BBa_K1362121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspGyrB C-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362431
component: BBa_K1362418
component: BBa_K1362407
component: BBa_K1362419
component: BBa_K1362421
component: BBa_K1362465
component: BBa_K1362422
component: BBa_K1362467
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362414
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362465
sequence annotation: BBa_K1362407
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362422
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362467
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362431
sequence: https://synbiohub.org/public/igem/BBa_K1362121_sequence/1

BBa_K1362431
type: Component
persistent identity: component2410440
display id: component2410440
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_K1362418
type: Component
persistent identity: component2410441
display id: component2410441
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362407
type: Component
persistent identity: component2410443
display id: component2410443
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362407/1

BBa_K1362419
type: Component
persistent identity: component2410444
display id: component2410444
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362421
type: Component
persistent identity: component2410467
display id: component2410467
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362465
type: Component
persistent identity: component2410468
display id: component2410468
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362422
type: Component
persistent identity: component2410465
display id: component2410465
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362467
type: Component
persistent identity: component2410442
display id: component2410442
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362467/1

BBa_G0000
type: Component
persistent identity: component2410436
display id: component2410436
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2410435
display id: component2410435
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362414
type: Component
persistent identity: component2410437
display id: component2410437
version: 1
top level: SspGyrB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362421
type: SequenceAnnotation
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display id: annotation2410467
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410467/range2410467/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410467/range2410467/1
type: Range
persistent identity: range2410467
display id: range2410467
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 1167
end: 1170
orientation: inline

BBa_K1362465
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version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410468/range2410468/1
component: BBa_K1362465

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type: Range
persistent identity: range2410468
display id: range2410468
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 1171
end: 1172
orientation: inline

BBa_K1362407
type: SequenceAnnotation
persistent identity: annotation2410443
display id: annotation2410443
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410443/range2410443/1
component: BBa_K1362407

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type: Range
persistent identity: range2410443
display id: range2410443
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 66
end: 79
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410437
display id: annotation2410437
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410437/range2410437/1
component: BBa_K1362414

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type: Range
persistent identity: range2410437
display id: range2410437
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 36
end: 38
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410465
display id: annotation2410465
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410465/range2410465/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410465/range2410465/1
type: Range
persistent identity: range2410465
display id: range2410465
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 84
end: 1166
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410436
display id: annotation2410436
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410436/range2410436/1
component: BBa_G0000

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type: Range
persistent identity: range2410436
display id: range2410436
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 30
end: 35
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410435
display id: annotation2410435
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410435/range2410435/1
component: BBa_K1362090

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type: Range
persistent identity: range2410435
display id: range2410435
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 1
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orientation: inline

BBa_K1362467
type: SequenceAnnotation
persistent identity: annotation2410442
display id: annotation2410442
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410442/range2410442/1
component: BBa_K1362467

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type: Range
persistent identity: range2410442
display id: range2410442
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 64
end: 65
orientation: inline

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2410441
display id: annotation2410441
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410441/range2410441/1
component: BBa_K1362418

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410441/range2410441/1
type: Range
persistent identity: range2410441
display id: range2410441
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 60
end: 63
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2410444
display id: annotation2410444
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410444/range2410444/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410444/range2410444/1
type: Range
persistent identity: range2410444
display id: range2410444
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 80
end: 83
orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2410440
display id: annotation2410440
version: 1
top level: SspGyrB(C)
location: https://synbiohub.org/public/igem/BBa_K1362121/annotation2410440/range2410440/1
component: BBa_K1362431

https://synbiohub.org/public/igem/BBa_K1362121/annotation2410440/range2410440/1
type: Range
persistent identity: range2410440
display id: range2410440
version: 1
direction: direction/reverse
top level: SspGyrB(C)
start: 39
end: 59
orientation: inline

SspGyrB(N)
type: ComponentDefinition
persistent identity: BBa K1362120
display id: BBa_K1362120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspGyrB N-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362437
component: BBa_K1362417
component: BBa_K1362421
component: BBa_K1362090
component: BBa_G0000
component: BBa_K1362422
component: BBa_K1362430
component: BBa_K1362415
component: BBa_K1362414
component: BBa_K1362406
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362406
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362414
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362421
sequence: https://synbiohub.org/public/igem/BBa_K1362120_sequence/1

BBa_K1362437
type: Component
persistent identity: component2410432
display id: component2410432
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362417
type: Component
persistent identity: component2410425
display id: component2410425
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362421
type: Component
persistent identity: component2410430
display id: component2410430
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362090
type: Component
persistent identity: component2410397
display id: component2410397
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_G0000
type: Component
persistent identity: component2410398
display id: component2410398
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362422
type: Component
persistent identity: component2410420
display id: component2410420
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362430
type: Component
persistent identity: component2410428
display id: component2410428
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362415
type: Component
persistent identity: component2410421
display id: component2410421
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362414
type: Component
persistent identity: component2410399
display id: component2410399
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362406
type: Component
persistent identity: component2410422
display id: component2410422
version: 1
top level: SspGyrB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362406/1

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410397
display id: annotation2410397
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410397/range2410397/1
component: BBa_K1362090

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type: Range
persistent identity: range2410397
display id: range2410397
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1
end: 29
orientation: inline

BBa_K1362406
type: SequenceAnnotation
persistent identity: annotation2410422
display id: annotation2410422
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410422/range2410422/1
component: BBa_K1362406

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410422/range2410422/1
type: Range
persistent identity: range2410422
display id: range2410422
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1126
end: 1571
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2410421
display id: annotation2410421
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410421/range2410421/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410421/range2410421/1
type: Range
persistent identity: range2410421
display id: range2410421
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1122
end: 1125
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410399
display id: annotation2410399
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410399/range2410399/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410399/range2410399/1
type: Range
persistent identity: range2410399
display id: range2410399
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 36
end: 38
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410398
display id: annotation2410398
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410398/range2410398/1
component: BBa_G0000

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type: Range
persistent identity: range2410398
display id: range2410398
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 30
end: 35
orientation: inline

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410425
display id: annotation2410425
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410425/range2410425/1
component: BBa_K1362417

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type: Range
persistent identity: range2410425
display id: range2410425
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1572
end: 1577
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410428
display id: annotation2410428
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410428/range2410428/1
component: BBa_K1362430

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type: Range
persistent identity: range2410428
display id: range2410428
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1578
end: 1601
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410432
display id: annotation2410432
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410432/range2410432/1
component: BBa_K1362437

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type: Range
persistent identity: range2410432
display id: range2410432
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1606
end: 1608
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410420
display id: annotation2410420
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410420/range2410420/1
component: BBa_K1362422

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type: Range
persistent identity: range2410420
display id: range2410420
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 39
end: 1121
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410430
display id: annotation2410430
version: 1
top level: SspGyrB(N)
location: https://synbiohub.org/public/igem/BBa_K1362120/annotation2410430/range2410430/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362120/annotation2410430/range2410430/1
type: Range
persistent identity: range2410430
display id: range2410430
version: 1
direction: direction/reverse
top level: SspGyrB(N)
start: 1602
end: 1605
orientation: inline

BBa_K1415101
type: ComponentDefinition
persistent identity: BBa K1415101
display id: BBa_K1415101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons+B0034+PBAN(Bombyx mori)
creator: HO, TSUNG YU
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has a constitutive promoter, so our PBAN(Bombyx) can constitutive express.
mutable notes: No
mutable provenance: Assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23101
component: BBa_K1415001
component: BBa_B0034
sequence annotation: BBa_K1415001
sequence annotation: BBa_B0034
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K1415101_sequence/1

BBa_J23101
type: Component
persistent identity: component2393960
display id: component2393960
version: 1
top level: BBa_K1415101
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K1415001
type: Component
persistent identity: component2393964
display id: component2393964
version: 1
top level: BBa_K1415101
access: public
definition: BBa_K1415001

BBa_B0034
type: Component
persistent identity: component2393962
display id: component2393962
version: 1
top level: BBa_K1415101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1415001
type: SequenceAnnotation
persistent identity: annotation2393964
display id: annotation2393964
version: 1
top level: BBa_K1415101
location: https://synbiohub.org/public/igem/BBa_K1415101/annotation2393964/range2393964/1
component: BBa_K1415001

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type: Range
persistent identity: range2393964
display id: range2393964
version: 1
direction: direction/reverse
top level: BBa_K1415101
start: 62
end: 170
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393962
display id: annotation2393962
version: 1
top level: BBa_K1415101
location: https://synbiohub.org/public/igem/BBa_K1415101/annotation2393962/range2393962/1
component: BBa_B0034

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type: Range
persistent identity: range2393962
display id: range2393962
version: 1
direction: direction/reverse
top level: BBa_K1415101
start: 44
end: 55
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2393960
display id: annotation2393960
version: 1
top level: BBa_K1415101
location: https://synbiohub.org/public/igem/BBa_K1415101/annotation2393960/range2393960/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1415101/annotation2393960/range2393960/1
type: Range
persistent identity: range2393960
display id: range2393960
version: 1
direction: direction/reverse
top level: BBa_K1415101
start: 1
end: 35
orientation: inline

BBa_K1336003
type: ComponentDefinition
persistent identity: BBa K1336003
display id: BBa_K1336003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dye-decolourising peroxidase from Bacillus subtilis
creator: Yan-Kay Ho
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1711_
m user id: 0
owner id: 20842
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: BsDyP gene (1251bp) from Bacillus subtilis that has been found to be effective in degrading lignin and azo dyes, in an oxidative process.
mutable notes: None.
mutable provenance: From genomic sequence of Bacillus subtilis; BsDyP gene inserted into a pET-21a (+) expression vector, provided to us by XXX Research Group.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA stop codon
sequence annotation: ATG start codon
sequence annotation: BsDyP coding region
sequence: https://synbiohub.org/public/igem/BBa_K1336003_sequence/1

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2397243
display id: annotation2397243
version: 1
top level: BBa_K1336003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1336003/annotation2397243/range2397243/1

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type: Range
persistent identity: range2397243
display id: range2397243
version: 1
direction: direction/forward
top level: BBa_K1336003
start: 1249
end: 1251
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2397242
display id: annotation2397242
version: 1
top level: BBa_K1336003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1336003/annotation2397242/range2397242/1

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type: Range
persistent identity: range2397242
display id: range2397242
version: 1
direction: direction/forward
top level: BBa_K1336003
start: 1
end: 3
orientation: inline

BsDyP coding region
type: SequenceAnnotation
persistent identity: annotation2397241
display id: annotation2397241
version: 1
top level: BBa_K1336003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1336003/annotation2397241/range2397241/1

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type: Range
persistent identity: range2397241
display id: range2397241
version: 1
direction: direction/forward
top level: BBa_K1336003
start: 1
end: 1251
orientation: inline

1GateNpuE
type: ComponentDefinition
persistent identity: BBa K1362101
display id: BBa_K1362101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+NpuDnaE C-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This intein assembly construct is part of the iGEM team Heidelberg 2014's strategy for cloning with split inteins. Inteins are naturally occuring peptide sequences that splice out of a precursor protein and attach the remaining ends together to form a new protein. When splitting those intein sequence into an N-terminal and a C-terminal split intein one is left with a powerful tool to post-translationally modify whole proteins on the amino-acid sequence level. This construct was designed to express any protein of interest fused to the Nostoc punctiforme DnaE N-terminal split intein. The corresponding C-terminal construct is BBa_K1362101 . Upon coexpression or mixture of the N- and C-constructs protein splicing takes place and the N- and C-terminal proteins of interest are irreversibly assembled via a newly formed peptide bond. This mechanism can be applied for a variety of different uses such as the activation of a protein through reconstitution of individually expressed split halves. See our [http://2014.igem.org/Team:Heidelberg split sfGFP experiment] and the respective parts in the registry for more information. Protein splicing offers many new possibilities and we hope to have set a foundation that you guys can build on! ===Nostoc punctiforme DnaE split intein (NpuDnaE)=== Inteins can be found in all kingdoms of life, however their use for the organism remains yet unknown. In the past years many intein sequences were found in the genomes of cyanobacteria. NpuDnaE is a naturally split intein from the alpha subunit of Dna Polymerase III in Nostoc punctiforme bacteria which shows [[#References|[2]]] extraordinarly high splicing activity and therefore is on of the gold-standards for inteins since many years. Amino Acid Sequence: N-Intein: AEY | CLSYETEILTVEYGLLPIGKIVEKRIECTVYSVDNNGNIYTQPVAQWHDRGEQEVFEYCLEDGSLIRATKDHKFMTVDGQMLPIDEIFERELDLMRVDNLPN C-Intein: MIKIATRKYLGKQNVYDIGVERDHNFALKNGFIASN | CFN
mutable notes: ???
mutable provenance: The part is assembled from different sources. The intein sequences come addgene or were kindly provided by different working groups. BBa_J04450 was used as mRFP selection marker. The scars and overhangs are part of the RFC[???] intein cloning strategy and are further explained on our [http://2014.igem.org/Team:Heidelberg/parts/rfc wiki] and in RFC[???] [[#Reference|[1]]]. Please see the corresponding basic parts for the individual detailed information.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362090
component: BBa_K1362418
component: BBa_K1362422
component: BBa_G0000
component: BBa_K1362414
component: BBa_K1362401
component: BBa_K1362465
component: BBa_K1362431
component: BBa_K1362421
component: BBa_K1362419
component: BBa_K1362464
sequence annotation: BBa_K1362419
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362464
sequence annotation: BBa_K1362401
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362465
sequence: https://synbiohub.org/public/igem/BBa_K1362101_sequence/1

BBa_K1362090
type: Component
persistent identity: component2410058
display id: component2410058
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362418
type: Component
persistent identity: component2410064
display id: component2410064
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362422
type: Component
persistent identity: component2410089
display id: component2410089
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_G0000
type: Component
persistent identity: component2410059
display id: component2410059
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362414
type: Component
persistent identity: component2410060
display id: component2410060
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362401
type: Component
persistent identity: component2410067
display id: component2410067
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362401/1

BBa_K1362465
type: Component
persistent identity: component2410092
display id: component2410092
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362431
type: Component
persistent identity: component2410063
display id: component2410063
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_K1362421
type: Component
persistent identity: component2410091
display id: component2410091
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362419
type: Component
persistent identity: component2410068
display id: component2410068
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362464
type: Component
persistent identity: component2410066
display id: component2410066
version: 1
top level: 1GateNpuE
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362464/1

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2410068
display id: annotation2410068
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410068/range2410068/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410068/range2410068/1
type: Range
persistent identity: range2410068
display id: range2410068
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 167
end: 170
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410059
display id: annotation2410059
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410059/range2410059/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410059/range2410059/1
type: Range
persistent identity: range2410059
display id: range2410059
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 30
end: 35
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410060
display id: annotation2410060
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410060/range2410060/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410060/range2410060/1
type: Range
persistent identity: range2410060
display id: range2410060
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 36
end: 38
orientation: inline

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2410064
display id: annotation2410064
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410064/range2410064/1
component: BBa_K1362418

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410064/range2410064/1
type: Range
persistent identity: range2410064
display id: range2410064
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 60
end: 63
orientation: inline

BBa_K1362464
type: SequenceAnnotation
persistent identity: annotation2410066
display id: annotation2410066
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410066/range2410066/1
component: BBa_K1362464

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410066/range2410066/1
type: Range
persistent identity: range2410066
display id: range2410066
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 64
end: 65
orientation: inline

BBa_K1362401
type: SequenceAnnotation
persistent identity: annotation2410067
display id: annotation2410067
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410067/range2410067/1
component: BBa_K1362401

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type: Range
persistent identity: range2410067
display id: range2410067
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 66
end: 166
orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2410063
display id: annotation2410063
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410063/range2410063/1
component: BBa_K1362431

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type: Range
persistent identity: range2410063
display id: range2410063
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 39
end: 59
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410089
display id: annotation2410089
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410089/range2410089/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410089/range2410089/1
type: Range
persistent identity: range2410089
display id: range2410089
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 171
end: 1253
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410091
display id: annotation2410091
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410091/range2410091/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410091/range2410091/1
type: Range
persistent identity: range2410091
display id: range2410091
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 1254
end: 1257
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410058
display id: annotation2410058
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410058/range2410058/1
component: BBa_K1362090

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type: Range
persistent identity: range2410058
display id: range2410058
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 1
end: 29
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2410092
display id: annotation2410092
version: 1
top level: 1GateNpuE
location: https://synbiohub.org/public/igem/BBa_K1362101/annotation2410092/range2410092/1
component: BBa_K1362465

https://synbiohub.org/public/igem/BBa_K1362101/annotation2410092/range2410092/1
type: Range
persistent identity: range2410092
display id: range2410092
version: 1
direction: direction/reverse
top level: 1GateNpuE
start: 1258
end: 1259
orientation: inline

BBa_K1321110
type: ComponentDefinition
persistent identity: BBa K1321110
display id: BBa_K1321110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to linker-dCBD with LacI
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phytochelatin EC(20) +CBD2 with LacI
mutable notes: Phytochelatin EC(20) +CBD2 with LacI
mutable provenance: Phytochelatin EC(20) +CBD2 with LacI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321005
component: BBa_K1321340
component: BBa_J04500
component: BBa_K1319106
sequence annotation: BBa_K1321340
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_J04500
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321110_sequence/1

BBa_B0105
type: Component
persistent identity: component2414868
display id: component2414868
version: 1
top level: BBa_K1321110
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: Component
persistent identity: component2414866
display id: component2414866
version: 1
top level: BBa_K1321110
access: public
definition: BBa_K1321005

BBa_K1321340
type: Component
persistent identity: component2414870
display id: component2414870
version: 1
top level: BBa_K1321110
access: public
definition: BBa_K1321340

BBa_J04500
type: Component
persistent identity: component2414862
display id: component2414862
version: 1
top level: BBa_K1321110
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1319106
type: Component
persistent identity: component2414864
display id: component2414864
version: 1
top level: BBa_K1321110
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414870
display id: annotation2414870
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414870/range2414870/1
component: BBa_K1321340

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type: Range
persistent identity: range2414870
display id: range2414870
version: 1
direction: direction/reverse
top level: BBa_K1321110
start: 365
end: 730
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414868
display id: annotation2414868
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414868/range2414868/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414868/range2414868/1
type: Range
persistent identity: range2414868
display id: range2414868
version: 1
direction: direction/reverse
top level: BBa_K1321110
start: 359
end: 364
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414866
display id: annotation2414866
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414866/range2414866/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414866/range2414866/1
type: Range
persistent identity: range2414866
display id: range2414866
version: 1
direction: direction/reverse
top level: BBa_K1321110
start: 236
end: 358
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414862
display id: annotation2414862
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414862/range2414862/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321110/annotation2414862/range2414862/1
type: Range
persistent identity: range2414862
display id: range2414862
version: 1
direction: direction/reverse
top level: BBa_K1321110
start: 1
end: 220
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414864
display id: annotation2414864
version: 1
top level: BBa_K1321110
location: https://synbiohub.org/public/igem/BBa_K1321110/annotation2414864/range2414864/1
component: BBa_K1319106

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type: Range
persistent identity: range2414864
display id: range2414864
version: 1
direction: direction/reverse
top level: BBa_K1321110
start: 221
end: 235
orientation: inline

NAGA
type: ComponentDefinition
persistent identity: BBa K1483000
display id: BBa_K1483000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: α-N-Acetylgalactosamindase
creator: Philipp H.O. Mayer and Philip Roessler
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1863_
m user id: 0
owner id: 21259
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Encodes for an enzyme, that is capable of cleaving off N-Acetlygalactosamine from A-group blood antigens, thereby turning them into H-antigens. Can be used to convert erythrocytes from blood type A to O. Part in RFC25 standard.
mutable notes: Part in RFC25, in order to ease fusion with other parts, for example immobilisation tags.
mutable provenance: The protein encoded by this sequence naturally occurs in Elizabethkingia meningoseptica( also called Chryseobacterium meningosepticum or Flavobacterium meningosepticum). This sequence was optimized for codon usage in E. coli K12 and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: N-Acetylgalactosaminidase
sequence: https://synbiohub.org/public/igem/BBa_K1483000_sequence/1

N-Acetylgalactosaminidase
type: SequenceAnnotation
persistent identity: annotation2396225
display id: annotation2396225
version: 1
top level: NAGA
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1483000/annotation2396225/range2396225/1

https://synbiohub.org/public/igem/BBa_K1483000/annotation2396225/range2396225/1
type: Range
persistent identity: range2396225
display id: range2396225
version: 1
direction: direction/forward
top level: NAGA
start: 1
end: 1329
orientation: inline

BBa_K1333313
type: ComponentDefinition
persistent identity: BBa K1333313
display id: BBa_K1333313
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PIII with an RNA thermometer(PrfA)
creator: Wei Tang
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15896
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: We add an araBAD promoter up to this part to regulate the PIII; expression wtih ara and temperature.
mutable notes: To regulate PIII; expression with temperature on the translation level used RNAT.
mutable provenance: The RNAT(PrfA) comes from bacteria genome and PIII; comes from M13 phage genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1333002
component: BBa_K115003
sequence annotation: BBa_K1333002
sequence annotation: BBa_K115003
sequence: https://synbiohub.org/public/igem/BBa_K1333313_sequence/1

BBa_K1333002
type: Component
persistent identity: component2386096
display id: component2386096
version: 1
top level: BBa_K1333313
access: public
definition: https://synbiohub.org/public/igem/BBa_K1333002/1

BBa_K115003
type: Component
persistent identity: component2386092
display id: component2386092
version: 1
top level: BBa_K1333313
access: public
definition: https://synbiohub.org/public/igem/BBa_K115003/1

BBa_K1333002
type: SequenceAnnotation
persistent identity: annotation2386096
display id: annotation2386096
version: 1
top level: BBa_K1333313
location: https://synbiohub.org/public/igem/BBa_K1333313/annotation2386096/range2386096/1
component: BBa_K1333002

https://synbiohub.org/public/igem/BBa_K1333313/annotation2386096/range2386096/1
type: Range
persistent identity: range2386096
display id: range2386096
version: 1
direction: direction/reverse
top level: BBa_K1333313
start: 116
end: 1456
orientation: inline

BBa_K115003
type: SequenceAnnotation
persistent identity: annotation2386092
display id: annotation2386092
version: 1
top level: BBa_K1333313
location: https://synbiohub.org/public/igem/BBa_K1333313/annotation2386092/range2386092/1
component: BBa_K115003

https://synbiohub.org/public/igem/BBa_K1333313/annotation2386092/range2386092/1
type: Range
persistent identity: range2386092
display id: range2386092
version: 1
direction: direction/reverse
top level: BBa_K1333313
start: 1
end: 109
orientation: inline

BBa_K1321345
type: ComponentDefinition
persistent identity: BBa K1321345
display id: BBa_K1321345
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to dCBD with linker in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:25:54Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321340
component: BBa_B0105
component: BBa_K1321009
sequence annotation: BBa_K1321009
sequence annotation: BBa_K1321340
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321345_sequence/1

BBa_K1321340
type: Component
persistent identity: component2415114
display id: component2415114
version: 1
top level: BBa_K1321345
access: public
definition: BBa_K1321340

BBa_B0105
type: Component
persistent identity: component2415112
display id: component2415112
version: 1
top level: BBa_K1321345
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321009
type: Component
persistent identity: component2415110
display id: component2415110
version: 1
top level: BBa_K1321345
access: public
definition: BBa_K1321009

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415110
display id: annotation2415110
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415110/range2415110/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321345/annotation2415110/range2415110/1
type: Range
persistent identity: range2415110
display id: range2415110
version: 1
direction: direction/reverse
top level: BBa_K1321345
start: 1
end: 176
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415114
display id: annotation2415114
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415114/range2415114/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321345/annotation2415114/range2415114/1
type: Range
persistent identity: range2415114
display id: range2415114
version: 1
direction: direction/reverse
top level: BBa_K1321345
start: 183
end: 548
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415112
display id: annotation2415112
version: 1
top level: BBa_K1321345
location: https://synbiohub.org/public/igem/BBa_K1321345/annotation2415112/range2415112/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321345/annotation2415112/range2415112/1
type: Range
persistent identity: range2415112
display id: range2415112
version: 1
direction: direction/reverse
top level: BBa_K1321345
start: 177
end: 182
orientation: inline

BBa_K1493803
type: ComponentDefinition
persistent identity: BBa K1493803
display id: BBa_K1493803
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter with CIλ and LacI operator sites
creator: Max van der Ploeg
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -34 binding site
sequence annotation: CI lambda operator site
sequence annotation: -10 binding site
sequence annotation: Lac operator site
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K1493803_sequence/1

-34 binding site
type: SequenceAnnotation
persistent identity: annotation2422446
display id: annotation2422446
version: 1
top level: BBa_K1493803
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1493803/annotation2422446/range2422446/1

https://synbiohub.org/public/igem/BBa_K1493803/annotation2422446/range2422446/1
type: Range
persistent identity: range2422446
display id: range2422446
version: 1
direction: direction/forward
top level: BBa_K1493803
start: 39
end: 43
orientation: inline

CI lambda operator site
type: SequenceAnnotation
persistent identity: annotation2422490
display id: annotation2422490
version: 1
top level: BBa_K1493803
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493803/annotation2422490/range2422490/1

https://synbiohub.org/public/igem/BBa_K1493803/annotation2422490/range2422490/1
type: Range
persistent identity: range2422490
display id: range2422490
version: 1
direction: direction/forward
top level: BBa_K1493803
start: 2
end: 17
orientation: inline

-10 binding site
type: SequenceAnnotation
persistent identity: annotation2422489
display id: annotation2422489
version: 1
top level: BBa_K1493803
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1493803/annotation2422489/range2422489/1

https://synbiohub.org/public/igem/BBa_K1493803/annotation2422489/range2422489/1
type: Range
persistent identity: range2422489
display id: range2422489
version: 1
direction: direction/forward
top level: BBa_K1493803
start: 60
end: 65
orientation: inline

Lac operator site
type: SequenceAnnotation
persistent identity: annotation2422492
display id: annotation2422492
version: 1
top level: BBa_K1493803
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493803/annotation2422492/range2422492/1

https://synbiohub.org/public/igem/BBa_K1493803/annotation2422492/range2422492/1
type: Range
persistent identity: range2422492
display id: range2422492
version: 1
direction: direction/forward
top level: BBa_K1493803
start: 44
end: 59
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2422447
display id: annotation2422447
version: 1
top level: BBa_K1493803
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1493803/annotation2422447/range2422447/1

https://synbiohub.org/public/igem/BBa_K1493803/annotation2422447/range2422447/1
type: Range
persistent identity: range2422447
display id: range2422447
version: 1
direction: direction/forward
top level: BBa_K1493803
start: 1
end: 104
orientation: inline

CI lambda operator site
type: SequenceAnnotation
persistent identity: annotation2422491
display id: annotation2422491
version: 1
top level: BBa_K1493803
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493803/annotation2422491/range2422491/1

https://synbiohub.org/public/igem/BBa_K1493803/annotation2422491/range2422491/1
type: Range
persistent identity: range2422491
display id: range2422491
version: 1
direction: direction/forward
top level: BBa_K1493803
start: 25
end: 40
orientation: inline

Lac operator site
type: SequenceAnnotation
persistent identity: annotation2422495
display id: annotation2422495
version: 1
top level: BBa_K1493803
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493803/annotation2422495/range2422495/1

https://synbiohub.org/public/igem/BBa_K1493803/annotation2422495/range2422495/1
type: Range
persistent identity: range2422495
display id: range2422495
version: 1
direction: direction/forward
top level: BBa_K1493803
start: 77
end: 97
orientation: inline

BBa_K1365998
type: ComponentDefinition
persistent identity: BBa K1365998
display id: BBa_K1365998
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL Generator that can be used in E.coli and L.lactis
creator: chandhuru Jagadeesan
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:07Z
discontinued: false
dominant: false
group u list: _1741_
m user id: 0
owner id: 20161
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -----
mutable notes: ------
mutable provenance: ------
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0015
component: BBa_K1033222
component: BBa_K081009
sequence annotation: BBa_K1033222
sequence annotation: BBa_B0015
sequence annotation: BBa_K081009
sequence: https://synbiohub.org/public/igem/BBa_K1365998_sequence/1

BBa_B0015
type: Component
persistent identity: component2401531
display id: component2401531
version: 1
top level: BBa_K1365998
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1033222
type: Component
persistent identity: component2401516
display id: component2401516
version: 1
top level: BBa_K1365998
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033222/1

BBa_K081009
type: Component
persistent identity: component2401524
display id: component2401524
version: 1
top level: BBa_K1365998
access: public
definition: https://synbiohub.org/public/igem/BBa_K081009/1

BBa_K1033222
type: SequenceAnnotation
persistent identity: annotation2401516
display id: annotation2401516
version: 1
top level: BBa_K1365998
location: https://synbiohub.org/public/igem/BBa_K1365998/annotation2401516/range2401516/1
component: BBa_K1033222

https://synbiohub.org/public/igem/BBa_K1365998/annotation2401516/range2401516/1
type: Range
persistent identity: range2401516
display id: range2401516
version: 1
direction: direction/reverse
top level: BBa_K1365998
start: 1
end: 59
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2401531
display id: annotation2401531
version: 1
top level: BBa_K1365998
location: https://synbiohub.org/public/igem/BBa_K1365998/annotation2401531/range2401531/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1365998/annotation2401531/range2401531/1
type: Range
persistent identity: range2401531
display id: range2401531
version: 1
direction: direction/reverse
top level: BBa_K1365998
start: 764
end: 892
orientation: inline

BBa_K081009
type: SequenceAnnotation
persistent identity: annotation2401524
display id: annotation2401524
version: 1
top level: BBa_K1365998
location: https://synbiohub.org/public/igem/BBa_K1365998/annotation2401524/range2401524/1
component: BBa_K081009

https://synbiohub.org/public/igem/BBa_K1365998/annotation2401524/range2401524/1
type: Range
persistent identity: range2401524
display id: range2401524
version: 1
direction: direction/reverse
top level: BBa_K1365998
start: 68
end: 755
orientation: inline

BBa_K1497026
type: ComponentDefinition
persistent identity: BBa K1497026
display id: BBa_K1497026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PDZ-Domain
creator: Sascha Hein, Rene Sahm
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497026_sequence/1

BBa_K1413021
type: ComponentDefinition
persistent identity: BBa K1413021
display id: BBa_K1413021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: bphR2 mutated
creator: Laura MATABISHI, Julie Zaworski
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1791_
m user id: 0
owner id: 22651
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: bphR2 gene, from Pseudomonas pseudoalcaligenes KF707, encodes for a regulatory protein (bphR2) which can detect polychlorinated biphenyls (PCBs). This gene regulates negatively bphR1 promoter involved in the transcription of biphenyl-catabolic genes (bph), degrading PCBs. In absence of PCB, bphR2 is bound to the bphR1 promoter. When compound diffuses into the cell, it binds to protein allowing a conformational change. BphR2 is release from the promoter that its allows the transcription of bph genes and therefore the degration of PCBs. Our aim was to finish and characterize the part of 2013 Paris Saclay's team. We used bphR2 in our biosensor project of PCBs. Instead of the genes of degradation, we have RFP (BBa_E1010) to detect 2 hydroxy-3',4' biphenyl.
mutable notes: Moreover, the sequence contained a pstI site from 142 to 148bp after ATG site so we have synthetized it by mutating the site, keeping the same amino acid.
mutable provenance: The gene was synthetized from the sequence of bphR2 (BBa_K1155009)because there isn't the sample in the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mutation
sequence annotation: bphR2
sequence: https://synbiohub.org/public/igem/BBa_K1413021_sequence/1

mutation
type: SequenceAnnotation
persistent identity: annotation2398817
display id: annotation2398817
version: 1
top level: BBa_K1413021
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1413021/annotation2398817/range2398817/1

https://synbiohub.org/public/igem/BBa_K1413021/annotation2398817/range2398817/1
type: Range
persistent identity: range2398817
display id: range2398817
version: 1
direction: direction/forward
top level: BBa_K1413021
start: 142
end: 147
orientation: inline

bphR2
type: SequenceAnnotation
persistent identity: annotation2398816
display id: annotation2398816
version: 1
top level: BBa_K1413021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1413021/annotation2398816/range2398816/1

https://synbiohub.org/public/igem/BBa_K1413021/annotation2398816/range2398816/1
type: Range
persistent identity: range2398816
display id: range2398816
version: 1
direction: direction/forward
top level: BBa_K1413021
start: 1
end: 903
orientation: inline

BBa_K1415107
type: ComponentDefinition
persistent identity: BBa K1415107
display id: BBa_K1415107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons+B0034+PBAN(Adoxophyes sp.)
creator: HO, TSUNG YU
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has a constitutive promoter, so our PBAN(Adoxophyes sp.) can constitutive express.
mutable notes: No
mutable provenance: Assemble
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1415007
component: BBa_J23101
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J23101
sequence annotation: BBa_K1415007
sequence: https://synbiohub.org/public/igem/BBa_K1415107_sequence/1

BBa_K1415007
type: Component
persistent identity: component2393989
display id: component2393989
version: 1
top level: BBa_K1415107
access: public
definition: BBa_K1415007

BBa_J23101
type: Component
persistent identity: component2393985
display id: component2393985
version: 1
top level: BBa_K1415107
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_B0034
type: Component
persistent identity: component2393987
display id: component2393987
version: 1
top level: BBa_K1415107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393987
display id: annotation2393987
version: 1
top level: BBa_K1415107
location: https://synbiohub.org/public/igem/BBa_K1415107/annotation2393987/range2393987/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1415107/annotation2393987/range2393987/1
type: Range
persistent identity: range2393987
display id: range2393987
version: 1
direction: direction/reverse
top level: BBa_K1415107
start: 44
end: 55
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2393985
display id: annotation2393985
version: 1
top level: BBa_K1415107
location: https://synbiohub.org/public/igem/BBa_K1415107/annotation2393985/range2393985/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1415107/annotation2393985/range2393985/1
type: Range
persistent identity: range2393985
display id: range2393985
version: 1
direction: direction/reverse
top level: BBa_K1415107
start: 1
end: 35
orientation: inline

BBa_K1415007
type: SequenceAnnotation
persistent identity: annotation2393989
display id: annotation2393989
version: 1
top level: BBa_K1415107
location: https://synbiohub.org/public/igem/BBa_K1415107/annotation2393989/range2393989/1
component: BBa_K1415007

https://synbiohub.org/public/igem/BBa_K1415107/annotation2393989/range2393989/1
type: Range
persistent identity: range2393989
display id: range2393989
version: 1
direction: direction/reverse
top level: BBa_K1415107
start: 62
end: 164
orientation: inline

BBa_K1362023
type: ComponentDefinition
persistent identity: BBa K1362023
display id: BBa_K1362023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Xylanase (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22920
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_G0000
component: BBa_K1362441
component: BBa_K1362414
component: BBa_K1362442
component: BBa_J70594
component: BBa_B0034
sequence annotation: BBa_K1362441
sequence annotation: BBa_B0034
sequence annotation: BBa_K1362442
sequence annotation: BBa_K1362414
sequence annotation: BBa_G0000
sequence annotation: BBa_J70594
sequence: https://synbiohub.org/public/igem/BBa_K1362023_sequence/1

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type: Component
persistent identity: component2409638
display id: component2409638
version: 1
top level: BBa_K1362023
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362441
type: Component
persistent identity: component2409641
display id: component2409641
version: 1
top level: BBa_K1362023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362441/1

BBa_K1362414
type: Component
persistent identity: component2409639
display id: component2409639
version: 1
top level: BBa_K1362023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362442
type: Component
persistent identity: component2409643
display id: component2409643
version: 1
top level: BBa_K1362023
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362442/1

BBa_J70594
type: Component
persistent identity: component2409645
display id: component2409645
version: 1
top level: BBa_K1362023
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_B0034
type: Component
persistent identity: component2409637
display id: component2409637
version: 1
top level: BBa_K1362023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1362441
type: SequenceAnnotation
persistent identity: annotation2409641
display id: annotation2409641
version: 1
top level: BBa_K1362023
location: https://synbiohub.org/public/igem/BBa_K1362023/annotation2409641/range2409641/1
component: BBa_K1362441

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type: Range
persistent identity: range2409641
display id: range2409641
version: 1
direction: direction/reverse
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start: 22
end: 576
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2409637
display id: annotation2409637
version: 1
top level: BBa_K1362023
location: https://synbiohub.org/public/igem/BBa_K1362023/annotation2409637/range2409637/1
component: BBa_B0034

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type: Range
persistent identity: range2409637
display id: range2409637
version: 1
direction: direction/reverse
top level: BBa_K1362023
start: 1
end: 12
orientation: inline

BBa_K1362442
type: SequenceAnnotation
persistent identity: annotation2409643
display id: annotation2409643
version: 1
top level: BBa_K1362023
location: https://synbiohub.org/public/igem/BBa_K1362023/annotation2409643/range2409643/1
component: BBa_K1362442

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type: Range
persistent identity: range2409643
display id: range2409643
version: 1
direction: direction/reverse
top level: BBa_K1362023
start: 577
end: 606
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2409639
display id: annotation2409639
version: 1
top level: BBa_K1362023
location: https://synbiohub.org/public/igem/BBa_K1362023/annotation2409639/range2409639/1
component: BBa_K1362414

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type: Range
persistent identity: range2409639
display id: range2409639
version: 1
direction: direction/reverse
top level: BBa_K1362023
start: 19
end: 21
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2409638
display id: annotation2409638
version: 1
top level: BBa_K1362023
location: https://synbiohub.org/public/igem/BBa_K1362023/annotation2409638/range2409638/1
component: BBa_G0000

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type: Range
persistent identity: range2409638
display id: range2409638
version: 1
direction: direction/reverse
top level: BBa_K1362023
start: 13
end: 18
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2409645
display id: annotation2409645
version: 1
top level: BBa_K1362023
location: https://synbiohub.org/public/igem/BBa_K1362023/annotation2409645/range2409645/1
component: BBa_J70594

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type: Range
persistent identity: range2409645
display id: range2409645
version: 1
direction: direction/reverse
top level: BBa_K1362023
start: 607
end: 612
orientation: inline

BBa_K1333321
type: ComponentDefinition
persistent identity: BBa K1333321
display id: BBa_K1333321
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13458-I13453-FourU-PⅢ
creator: Wei Tang
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15896
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: I13458-I13453-FourU-PⅢ
mutable notes: I13458-I13453-FourU-PⅢ
mutable provenance: I13458-I13453-FourU-PⅢ
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I13453
component: BBa_K115002
component: BBa_I13458
component: BBa_K1333002
sequence annotation: BBa_I13458
sequence annotation: BBa_K115002
sequence annotation: BBa_K1333002
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1333321_sequence/1

BBa_I13453
type: Component
persistent identity: component2407897
display id: component2407897
version: 1
top level: BBa_K1333321
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K115002
type: Component
persistent identity: component2407902
display id: component2407902
version: 1
top level: BBa_K1333321
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_I13458
type: Component
persistent identity: component2407896
display id: component2407896
version: 1
top level: BBa_K1333321
access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_K1333002
type: Component
persistent identity: component2407906
display id: component2407906
version: 1
top level: BBa_K1333321
access: public
definition: https://synbiohub.org/public/igem/BBa_K1333002/1

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation2407896
display id: annotation2407896
version: 1
top level: BBa_K1333321
location: https://synbiohub.org/public/igem/BBa_K1333321/annotation2407896/range2407896/1
component: BBa_I13458

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type: Range
persistent identity: range2407896
display id: range2407896
version: 1
direction: direction/reverse
top level: BBa_K1333321
start: 1
end: 1114
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2407902
display id: annotation2407902
version: 1
top level: BBa_K1333321
location: https://synbiohub.org/public/igem/BBa_K1333321/annotation2407902/range2407902/1
component: BBa_K115002

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type: Range
persistent identity: range2407902
display id: range2407902
version: 1
direction: direction/reverse
top level: BBa_K1333321
start: 1261
end: 1312
orientation: inline

BBa_K1333002
type: SequenceAnnotation
persistent identity: annotation2407906
display id: annotation2407906
version: 1
top level: BBa_K1333321
location: https://synbiohub.org/public/igem/BBa_K1333321/annotation2407906/range2407906/1
component: BBa_K1333002

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type: Range
persistent identity: range2407906
display id: range2407906
version: 1
direction: direction/reverse
top level: BBa_K1333321
start: 1319
end: 2659
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2407897
display id: annotation2407897
version: 1
top level: BBa_K1333321
location: https://synbiohub.org/public/igem/BBa_K1333321/annotation2407897/range2407897/1
component: BBa_I13453

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type: Range
persistent identity: range2407897
display id: range2407897
version: 1
direction: direction/reverse
top level: BBa_K1333321
start: 1123
end: 1252
orientation: inline

BBa_K1321366
type: ComponentDefinition
persistent identity: BBa K1321366
display id: BBa_K1321366
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to sfGFP with T7 promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2016-01-26T09:46:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321338
component: BBa_K1319106
component: BBa_K1321349
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1321349
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321366_sequence/1

BBa_K1321338
type: Component
persistent identity: component2415724
display id: component2415724
version: 1
top level: BBa_K1321366
access: public
definition: BBa_K1321338

BBa_K1319106
type: Component
persistent identity: component2415726
display id: component2415726
version: 1
top level: BBa_K1321366
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321349
type: Component
persistent identity: component2415732
display id: component2415732
version: 1
top level: BBa_K1321366
access: public
definition: BBa_K1321349

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415724
display id: annotation2415724
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415724/range2415724/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415724/range2415724/1
type: Range
persistent identity: range2415724
display id: range2415724
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 1
end: 43
orientation: inline

BBa_K1321349
type: SequenceAnnotation
persistent identity: annotation2415732
display id: annotation2415732
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415732/range2415732/1
component: BBa_K1321349

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type: Range
persistent identity: range2415732
display id: range2415732
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 59
end: 1102
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415726
display id: annotation2415726
version: 1
top level: BBa_K1321366
location: https://synbiohub.org/public/igem/BBa_K1321366/annotation2415726/range2415726/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321366/annotation2415726/range2415726/1
type: Range
persistent identity: range2415726
display id: range2415726
version: 1
direction: direction/reverse
top level: BBa_K1321366
start: 44
end: 58
orientation: inline

BBa_K1351029
type: ComponentDefinition
persistent identity: BBa K1351029
display id: BBa_K1351029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LMU Bacillus RBS collection 2
creator: Florian Riemer
created: 2014-10-05T11:00:00Z
modified: 2015-06-01T08:10:03Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20062
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: "This part was generated in a modified version of RFC25, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGATGGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined."
mutable notes: The LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1351029_sequence/1

BBa_K1349008
type: ComponentDefinition
persistent identity: BBa K1349008
display id: BBa_K1349008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sh3pep-lza
creator: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1724_
m user id: 0
owner id: 6328
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SH3pep-LZA is a link wich mediates proteins interactions.
mutable notes: xxx
mutable provenance: Amplified from pWW2181,Whitaker et al. PNAS 2012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1349008_sequence/1

BBa_K1362020
type: ComponentDefinition
persistent identity: BBa K1362020
display id: BBa_K1362020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Xylanase
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22920
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1362414
component: BBa_J70594
component: BBa_B0034
component: BBa_G0000
component: BBa_K1362441
sequence annotation: BBa_J70594
sequence annotation: BBa_B0034
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362441
sequence annotation: BBa_G0000
sequence: https://synbiohub.org/public/igem/BBa_K1362020_sequence/1

BBa_K1362414
type: Component
persistent identity: component2403702
display id: component2403702
version: 1
top level: BBa_K1362020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_J70594
type: Component
persistent identity: component2403705
display id: component2403705
version: 1
top level: BBa_K1362020
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_B0034
type: Component
persistent identity: component2403700
display id: component2403700
version: 1
top level: BBa_K1362020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_G0000
type: Component
persistent identity: component2403701
display id: component2403701
version: 1
top level: BBa_K1362020
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362441
type: Component
persistent identity: component2403703
display id: component2403703
version: 1
top level: BBa_K1362020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362441/1

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2403705
display id: annotation2403705
version: 1
top level: BBa_K1362020
location: https://synbiohub.org/public/igem/BBa_K1362020/annotation2403705/range2403705/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362020/annotation2403705/range2403705/1
type: Range
persistent identity: range2403705
display id: range2403705
version: 1
direction: direction/reverse
top level: BBa_K1362020
start: 577
end: 582
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2403700
display id: annotation2403700
version: 1
top level: BBa_K1362020
location: https://synbiohub.org/public/igem/BBa_K1362020/annotation2403700/range2403700/1
component: BBa_B0034

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type: Range
persistent identity: range2403700
display id: range2403700
version: 1
direction: direction/reverse
top level: BBa_K1362020
start: 1
end: 12
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2403702
display id: annotation2403702
version: 1
top level: BBa_K1362020
location: https://synbiohub.org/public/igem/BBa_K1362020/annotation2403702/range2403702/1
component: BBa_K1362414

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type: Range
persistent identity: range2403702
display id: range2403702
version: 1
direction: direction/reverse
top level: BBa_K1362020
start: 19
end: 21
orientation: inline

BBa_K1362441
type: SequenceAnnotation
persistent identity: annotation2403703
display id: annotation2403703
version: 1
top level: BBa_K1362020
location: https://synbiohub.org/public/igem/BBa_K1362020/annotation2403703/range2403703/1
component: BBa_K1362441

https://synbiohub.org/public/igem/BBa_K1362020/annotation2403703/range2403703/1
type: Range
persistent identity: range2403703
display id: range2403703
version: 1
direction: direction/reverse
top level: BBa_K1362020
start: 22
end: 576
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2403701
display id: annotation2403701
version: 1
top level: BBa_K1362020
location: https://synbiohub.org/public/igem/BBa_K1362020/annotation2403701/range2403701/1
component: BBa_G0000

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type: Range
persistent identity: range2403701
display id: range2403701
version: 1
direction: direction/reverse
top level: BBa_K1362020
start: 13
end: 18
orientation: inline

BBa_K1330002
type: ComponentDefinition
persistent identity: BBa K1330002
display id: BBa_K1330002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter (J23105)
creator: Kristian Jensen
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1705_
m user id: 0
owner id: 20126
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1330002_sequence/1

BBa_K1365020
type: ComponentDefinition
persistent identity: BBa K1365020
display id: BBa_K1365020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP(Bs)
creator: Sandra Mous
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:07Z
discontinued: false
dominant: false
group u list: _1741_
m user id: 0
owner id: 23000
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Codes for the sfGFP protein, an optimized fluorescent reporter. Was originally optimized for Bacillus subtilis, but then showed high fluorescence in Lactococcus lactis.
mutable notes: Used primers: forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT-3' reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT-3' to PCR sfGFP(Bs) from the plasmid and add the BioBrick prefix and suffix.
mutable provenance: PCR on plasmid MG_sfgfp(Bs), used in the study 'Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging', see references. Plasmid kindly provided by the Molecular Genetics group of the Rijksuniversiteit Groningen.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: sfGFP(Bs)
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1365020_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2420001
display id: annotation2420001
version: 1
top level: BBa_K1365020
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1365020/annotation2420001/range2420001/1

https://synbiohub.org/public/igem/BBa_K1365020/annotation2420001/range2420001/1
type: Range
persistent identity: range2420001
display id: range2420001
version: 1
direction: direction/forward
top level: BBa_K1365020
start: 715
end: 717
orientation: inline

sfGFP(Bs)
type: SequenceAnnotation
persistent identity: annotation2420000
display id: annotation2420000
version: 1
top level: BBa_K1365020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1365020/annotation2420000/range2420000/1

https://synbiohub.org/public/igem/BBa_K1365020/annotation2420000/range2420000/1
type: Range
persistent identity: range2420000
display id: range2420000
version: 1
direction: direction/forward
top level: BBa_K1365020
start: 1
end: 717
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2419999
display id: annotation2419999
version: 1
top level: BBa_K1365020
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1365020/annotation2419999/range2419999/1

https://synbiohub.org/public/igem/BBa_K1365020/annotation2419999/range2419999/1
type: Range
persistent identity: range2419999
display id: range2419999
version: 1
direction: direction/forward
top level: BBa_K1365020
start: 1
end: 1
orientation: inline

BBa_K1497010
type: ComponentDefinition
persistent identity: BBa K1497010
display id: BBa_K1497010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Dihydroflavonol 4-reductase (DFR)
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497010_sequence/1

BBa_K1351034
type: ComponentDefinition
persistent identity: BBa K1351034
display id: BBa_K1351034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LMU Bacillus RBS collection 7
creator: Florian Riemer
created: 2014-10-05T11:00:00Z
modified: 2015-06-01T07:44:23Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20062
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: "This part was generated in a modified version of RFC25, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGATGGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined."
mutable notes: The LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1351034_sequence/1

BBa_K1486022
type: ComponentDefinition
persistent identity: BBa K1486022
display id: BBa_K1486022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Renilla Luciferase
creator: EPFL iGem team 2014
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1866_
m user id: 0
owner id: 21042
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the full renilla luciferase. The split protein has also been submitted as biobricks: BBa_K1486021.
mutable notes: to edit
mutable provenance: To edit
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_K1486019
component: BBa_K1486020
component: BBa_I0500
sequence annotation: BBa_K1486019
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_K1486020
sequence: https://synbiohub.org/public/igem/BBa_K1486022_sequence/1

BBa_B0034
type: Component
persistent identity: component2383473
display id: component2383473
version: 1
top level: BBa_K1486022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1486019
type: Component
persistent identity: component2383474
display id: component2383474
version: 1
top level: BBa_K1486022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486019/1

BBa_K1486020
type: Component
persistent identity: component2383475
display id: component2383475
version: 1
top level: BBa_K1486022
access: public
definition: https://synbiohub.org/public/igem/BBa_K1486020/1

BBa_I0500
type: Component
persistent identity: component2383471
display id: component2383471
version: 1
top level: BBa_K1486022
access: public
definition: pBad/araC

BBa_K1486019
type: SequenceAnnotation
persistent identity: annotation2383474
display id: annotation2383474
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383474/range2383474/1
component: BBa_K1486019

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383474/range2383474/1
type: Range
persistent identity: range2383474
display id: range2383474
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1223
end: 1552
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2383471
display id: annotation2383471
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383471/range2383471/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383471/range2383471/1
type: Range
persistent identity: range2383471
display id: range2383471
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1
end: 1210
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383473
display id: annotation2383473
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383473/range2383473/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383473/range2383473/1
type: Range
persistent identity: range2383473
display id: range2383473
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1211
end: 1222
orientation: inline

BBa_K1486020
type: SequenceAnnotation
persistent identity: annotation2383475
display id: annotation2383475
version: 1
top level: BBa_K1486022
location: https://synbiohub.org/public/igem/BBa_K1486022/annotation2383475/range2383475/1
component: BBa_K1486020

https://synbiohub.org/public/igem/BBa_K1486022/annotation2383475/range2383475/1
type: Range
persistent identity: range2383475
display id: range2383475
version: 1
direction: direction/reverse
top level: BBa_K1486022
start: 1553
end: 2158
orientation: inline

BBa_K1321348
type: ComponentDefinition
persistent identity: BBa K1321348
display id: BBa_K1321348
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to dCBD with linker in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:41Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321340
component: BBa_K1321337
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1321337
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321348_sequence/1

BBa_B0105
type: Component
persistent identity: component2415138
display id: component2415138
version: 1
top level: BBa_K1321348
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321340
type: Component
persistent identity: component2415140
display id: component2415140
version: 1
top level: BBa_K1321348
access: public
definition: BBa_K1321340

BBa_K1321337
type: Component
persistent identity: component2415136
display id: component2415136
version: 1
top level: BBa_K1321348
access: public
definition: BBa_K1321337

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415140
display id: annotation2415140
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415140/range2415140/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415140/range2415140/1
type: Range
persistent identity: range2415140
display id: range2415140
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 718
end: 1083
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415136
display id: annotation2415136
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415136/range2415136/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415136/range2415136/1
type: Range
persistent identity: range2415136
display id: range2415136
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 1
end: 711
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415138
display id: annotation2415138
version: 1
top level: BBa_K1321348
location: https://synbiohub.org/public/igem/BBa_K1321348/annotation2415138/range2415138/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321348/annotation2415138/range2415138/1
type: Range
persistent identity: range2415138
display id: range2415138
version: 1
direction: direction/reverse
top level: BBa_K1321348
start: 712
end: 717
orientation: inline

BBa_K1415008
type: ComponentDefinition
persistent identity: BBa K1415008
display id: BBa_K1415008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAN (Solenopsis invicta)
creator: Min-Chien Hsiao
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22254
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Red imported fire ant (Solenopsis invicta) Spread:The red imported fire ant, a eusocial species, are far more aggressive than most ant species. Animals, including humans, often encounter them by inadvertently stepping on one of their mounds, which causes the ants to swarm up the legs, attacking en masse. The ants respond to pheromones released by the first ant that attacks, thereafter stinging in concert. Characteristics: Fire ants are red and black in coloration and, like all insects, they are protected by a hard exoskeleton and have six legs. Worker ants have round heads with mandibles, an armored thorax midsection and an abdomen, made up of the pedicle and the gaster. The head is typically copper brown in color. In addition to their mandibles, fire ant workers also possess an abdominal stinger. Damage: They are considered to be a pest, not only because of the physical pain they can inflict, but also because their mound-building activity can damage plant roots, lead to loss of crops Control:(2) Hot water Pouring hot water on the mounds is effective and environmentally friendly, but may require 3 or 4 applications to kill the colony. Water should be at least scalding hot, but does not need to be boiling. This works best when you use 3 to 4 gallons of water in each application. WARNING: Hot water kills grass and shrubbery and may cause severe burns if spilled. (2)Liquid nitrogen: it could be the most effective and most environmental friendly method to eradicate the species.
mutable notes: No
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PBAN (Solenopsis invicta)
sequence: https://synbiohub.org/public/igem/BBa_K1415008_sequence/1

PBAN (Solenopsis invicta)
type: SequenceAnnotation
persistent identity: annotation2392763
display id: annotation2392763
version: 1
top level: BBa_K1415008
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1415008/annotation2392763/range2392763/1

https://synbiohub.org/public/igem/BBa_K1415008/annotation2392763/range2392763/1
type: Range
persistent identity: range2392763
display id: range2392763
version: 1
direction: direction/forward
top level: BBa_K1415008
start: 1
end: 88
orientation: inline

BBa_K1423006
type: ComponentDefinition
persistent identity: BBa K1423006
display id: BBa_K1423006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Arsenic Inducible Banana Odor Generator
creator: Kayla DeSanty
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
group u list: _1801_
m user id: 0
owner id: 22282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The aresnic inducible banana odor generator converts isoamyl alcohol to isoamyl acetate in the presence of arsenic.
mutable notes: We digested the Biobricks with CIP to assure that the plasmids did not re-ligate to themselves.
mutable provenance: The arsenic promoter with the arsR repressor gene is naturally found in E. coli. The ribosome binding site, the ATF1 gene, and the double terminator combined sequence was obtained from the Biobrick BBa_J45199.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J45199
component: BBa_J33201
sequence annotation: BBa_J45199
sequence annotation: BBa_J33201
sequence: https://synbiohub.org/public/igem/BBa_K1423006_sequence/1

BBa_J45199
type: Component
persistent identity: component2385476
display id: component2385476
version: 1
top level: BBa_K1423006
access: public
definition: https://synbiohub.org/public/igem/BBa_J45199/1

BBa_J33201
type: Component
persistent identity: component2385461
display id: component2385461
version: 1
top level: BBa_K1423006
access: public
definition: https://synbiohub.org/public/igem/BBa_J33201/1

BBa_J45199
type: SequenceAnnotation
persistent identity: annotation2385476
display id: annotation2385476
version: 1
top level: BBa_K1423006
location: https://synbiohub.org/public/igem/BBa_K1423006/annotation2385476/range2385476/1
component: BBa_J45199

https://synbiohub.org/public/igem/BBa_K1423006/annotation2385476/range2385476/1
type: Range
persistent identity: range2385476
display id: range2385476
version: 1
direction: direction/reverse
top level: BBa_K1423006
start: 527
end: 2265
orientation: inline

BBa_J33201
type: SequenceAnnotation
persistent identity: annotation2385461
display id: annotation2385461
version: 1
top level: BBa_K1423006
location: https://synbiohub.org/public/igem/BBa_K1423006/annotation2385461/range2385461/1
component: BBa_J33201

https://synbiohub.org/public/igem/BBa_K1423006/annotation2385461/range2385461/1
type: Range
persistent identity: range2385461
display id: range2385461
version: 1
direction: direction/reverse
top level: BBa_K1423006
start: 1
end: 518
orientation: inline

aGal
type: ComponentDefinition
persistent identity: BBa K1483002
display id: BBa_K1483002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: α-Galactosidase
creator: Philipp H. O. Mayer and Philip Roessler
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1863_
m user id: 0
owner id: 21259
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Encodes for an enzyme, that is capable of cleaving off galactose from B-group blood antigens, thereby turning them into H-antigens. Can be used to convert erythrocytes from blood type A to O. Part in RFC25 standard.
mutable notes: Part in RFC25, in order to ease fusion with other parts, for example immobilisation tags.
mutable provenance: The protein encoded by this sequence naturally occurs in Bacteroides fragilis. This sequence was optimized for codon usage in E. coli K12 and synthesized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: alpha-Galactosidase
sequence: https://synbiohub.org/public/igem/BBa_K1483002_sequence/1

alpha-Galactosidase
type: SequenceAnnotation
persistent identity: annotation2396465
display id: annotation2396465
version: 1
top level: aGal
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1483002/annotation2396465/range2396465/1

https://synbiohub.org/public/igem/BBa_K1483002/annotation2396465/range2396465/1
type: Range
persistent identity: range2396465
display id: range2396465
version: 1
direction: direction/forward
top level: aGal
start: 1
end: 1752
orientation: inline

BBa_K1375019
type: ComponentDefinition
persistent identity: BBa K1375019
display id: BBa_K1375019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prhl/las +RBS+eforRed
creator: Zheng Xu
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
group u list: _1752_
m user id: 0
owner id: 18474
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a regulated promoter with a eforRed protein
mutable notes: no
mutable provenance: K1375024 K592012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1375024
component: BBa_K592012
sequence annotation: BBa_K592012
sequence annotation: BBa_K1375024
sequence: https://synbiohub.org/public/igem/BBa_K1375019_sequence/1

BBa_K1375024
type: Component
persistent identity: component2416883
display id: component2416883
version: 1
top level: BBa_K1375019
access: public
definition: https://synbiohub.org/public/igem/BBa_K1375024/1

BBa_K592012
type: Component
persistent identity: component2416885
display id: component2416885
version: 1
top level: BBa_K1375019
access: public
definition: https://synbiohub.org/public/igem/BBa_K592012/1

BBa_K592012
type: SequenceAnnotation
persistent identity: annotation2416885
display id: annotation2416885
version: 1
top level: BBa_K1375019
location: https://synbiohub.org/public/igem/BBa_K1375019/annotation2416885/range2416885/1
component: BBa_K592012

https://synbiohub.org/public/igem/BBa_K1375019/annotation2416885/range2416885/1
type: Range
persistent identity: range2416885
display id: range2416885
version: 1
direction: direction/reverse
top level: BBa_K1375019
start: 82
end: 762
orientation: inline

BBa_K1375024
type: SequenceAnnotation
persistent identity: annotation2416883
display id: annotation2416883
version: 1
top level: BBa_K1375019
location: https://synbiohub.org/public/igem/BBa_K1375019/annotation2416883/range2416883/1
component: BBa_K1375024

https://synbiohub.org/public/igem/BBa_K1375019/annotation2416883/range2416883/1
type: Range
persistent identity: range2416883
display id: range2416883
version: 1
direction: direction/reverse
top level: BBa_K1375019
start: 1
end: 75
orientation: inline

SAMmt
type: ComponentDefinition
persistent identity: BBa K1312005
display id: BBa_K1312005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S-adenosyl methionine-dependent methyltransferase relate to Met-DMSP byosynthesis pathway(SAMmt)
creator: Yutaka Murata
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1687_
m user id: 0
owner id: 17633
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is S-adenosyl methionine-dependent methyltransferase gene of Fragilariopsis cylindrus. The gene code S-adenosyl methionine-dependent methyltransferase relate to Met-DMSP byosynthesis pathway from Fragilariopsis cylindrus. which catalyze third step of Met-DMSP biosynthesis pathway.
mutable notes: This part was excluded intron. And there was PstⅠ recognition site so I made single base substitution in recognition site.
mutable provenance: From Fragilariopsis cylindrus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SAMmt
sequence: https://synbiohub.org/public/igem/BBa_K1312005_sequence/1

SAMmt
type: SequenceAnnotation
persistent identity: annotation2428845
display id: annotation2428845
version: 1
top level: SAMmt
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1312005/annotation2428845/range2428845/1

https://synbiohub.org/public/igem/BBa_K1312005/annotation2428845/range2428845/1
type: Range
persistent identity: range2428845
display id: range2428845
version: 1
direction: direction/forward
top level: SAMmt
start: 1
end: 1315
orientation: inline

BBa_K1493806
type: ComponentDefinition
persistent identity: BBa K1493806
display id: BBa_K1493806
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter with CIλ and LacI operator sites
creator: Max van der Ploeg
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -34 binding site
sequence annotation: promoter
sequence annotation: cI lambda operator site
sequence annotation: -10 binding site
sequence annotation: lac operator site
sequence annotation: Lac operator site
sequence: https://synbiohub.org/public/igem/BBa_K1493806_sequence/1

-34 binding site
type: SequenceAnnotation
persistent identity: annotation2422562
display id: annotation2422562
version: 1
top level: BBa_K1493806
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1493806/annotation2422562/range2422562/1

https://synbiohub.org/public/igem/BBa_K1493806/annotation2422562/range2422562/1
type: Range
persistent identity: range2422562
display id: range2422562
version: 1
direction: direction/forward
top level: BBa_K1493806
start: 38
end: 42
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2422560
display id: annotation2422560
version: 1
top level: BBa_K1493806
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1493806/annotation2422560/range2422560/1

https://synbiohub.org/public/igem/BBa_K1493806/annotation2422560/range2422560/1
type: Range
persistent identity: range2422560
display id: range2422560
version: 1
direction: direction/forward
top level: BBa_K1493806
start: 1
end: 103
orientation: inline

cI lambda operator site
type: SequenceAnnotation
persistent identity: annotation2422566
display id: annotation2422566
version: 1
top level: BBa_K1493806
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493806/annotation2422566/range2422566/1

https://synbiohub.org/public/igem/BBa_K1493806/annotation2422566/range2422566/1
type: Range
persistent identity: range2422566
display id: range2422566
version: 1
direction: direction/forward
top level: BBa_K1493806
start: 47
end: 62
orientation: inline

-10 binding site
type: SequenceAnnotation
persistent identity: annotation2422564
display id: annotation2422564
version: 1
top level: BBa_K1493806
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1493806/annotation2422564/range2422564/1

https://synbiohub.org/public/igem/BBa_K1493806/annotation2422564/range2422564/1
type: Range
persistent identity: range2422564
display id: range2422564
version: 1
direction: direction/forward
top level: BBa_K1493806
start: 59
end: 64
orientation: inline

lac operator site
type: SequenceAnnotation
persistent identity: annotation2422565
display id: annotation2422565
version: 1
top level: BBa_K1493806
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493806/annotation2422565/range2422565/1

https://synbiohub.org/public/igem/BBa_K1493806/annotation2422565/range2422565/1
type: Range
persistent identity: range2422565
display id: range2422565
version: 1
direction: direction/forward
top level: BBa_K1493806
start: 20
end: 37
orientation: inline

Lac operator site
type: SequenceAnnotation
persistent identity: annotation2422567
display id: annotation2422567
version: 1
top level: BBa_K1493806
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493806/annotation2422567/range2422567/1

https://synbiohub.org/public/igem/BBa_K1493806/annotation2422567/range2422567/1
type: Range
persistent identity: range2422567
display id: range2422567
version: 1
direction: direction/forward
top level: BBa_K1493806
start: 75
end: 97
orientation: inline

BBa_K1415104
type: ComponentDefinition
persistent identity: BBa K1415104
display id: BBa_K1415104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons+B0034+PBAN(Lymantria dispar)
creator: HO, TSUNG YU
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has a constitutive promoter, so our PBAN(Lymantria dispar) can constitutive express.
mutable notes: No
mutable provenance: Assemble
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1415004
component: BBa_J23101
sequence annotation: BBa_B0034
sequence annotation: BBa_J23101
sequence annotation: BBa_K1415004
sequence: https://synbiohub.org/public/igem/BBa_K1415104_sequence/1

BBa_B0034
type: Component
persistent identity: component2393972
display id: component2393972
version: 1
top level: BBa_K1415104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1415004
type: Component
persistent identity: component2393974
display id: component2393974
version: 1
top level: BBa_K1415104
access: public
definition: BBa_K1415004

BBa_J23101
type: Component
persistent identity: component2393970
display id: component2393970
version: 1
top level: BBa_K1415104
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393972
display id: annotation2393972
version: 1
top level: BBa_K1415104
location: https://synbiohub.org/public/igem/BBa_K1415104/annotation2393972/range2393972/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1415104/annotation2393972/range2393972/1
type: Range
persistent identity: range2393972
display id: range2393972
version: 1
direction: direction/reverse
top level: BBa_K1415104
start: 44
end: 55
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2393970
display id: annotation2393970
version: 1
top level: BBa_K1415104
location: https://synbiohub.org/public/igem/BBa_K1415104/annotation2393970/range2393970/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1415104/annotation2393970/range2393970/1
type: Range
persistent identity: range2393970
display id: range2393970
version: 1
direction: direction/reverse
top level: BBa_K1415104
start: 1
end: 35
orientation: inline

BBa_K1415004
type: SequenceAnnotation
persistent identity: annotation2393974
display id: annotation2393974
version: 1
top level: BBa_K1415104
location: https://synbiohub.org/public/igem/BBa_K1415104/annotation2393974/range2393974/1
component: BBa_K1415004

https://synbiohub.org/public/igem/BBa_K1415104/annotation2393974/range2393974/1
type: Range
persistent identity: range2393974
display id: range2393974
version: 1
direction: direction/reverse
top level: BBa_K1415104
start: 62
end: 170
orientation: inline

BBa_K1497032
type: ComponentDefinition
persistent identity: BBa K1497032
display id: BBa_K1497032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold (G-S-P-His-Cys)
creator: Sascha Hein, Rene Sahm, Malte Blumenroth, Tobias Gabriel, Sebastian Palluk
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Scaffold (G-S-P-His-Cys)
mutable notes: Scaffold (G-S-P-His-Cys)
mutable provenance: Scaffold (G-S-P-His-Cys)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497032_sequence/1

BBa_K1351002
type: ComponentDefinition
persistent identity: BBa K1351002
display id: BBa_K1351002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chondroitin 4 sulfotransferase-derived peptide binding to NADH Oxidase of S. pneumoniae
creator: Mona Dotzler
created: 2014-10-02T11:00:00Z
modified: 2015-06-01T12:53:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Short peptide of 13 amino acids corresponding to amino acids 279 to 290 of the human protein Chondroitin 4 sulfotransferase (UniProt [http://www.uniprot.org/uniprot/Q9NRB3 Q9NRB3]). It has been shown to inhibit NADH oxidase (NOX) mediated binding of ''Streptococcus pneumoniae'' to human A549 cells (Muchnik ''et. al.'', 2013). Displayed on the surface of ''Bacillus subtilis'', it should enable ''B. subtilis'' to bind specifically to ''S. pneumoniae''. This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
mutable notes: For generation of the part's nucleotide sequence, the amino acid sequence of the peptide (Muchnik et. al., 2013) was reverse transcribed with regard to the codon usage of Bacillus subtilis. Oligos were designed as precut by EcoRI and PstI to shorten the synthesized fragments and to simplify the cloning into pSB1C3.
mutable provenance: The part was generated by annealing of artificially synthesized oligonucletides: CSP_ENX_SD_Ngo_precutEP_fwd: AATTCgcggccgctTCTAGAgtaaggaggaaGCCGGCATG GCGAACCACACATCACTTCCGGCGTCAGCGAGAGAA ACCGGTtaatACTAGTagcggccgCTGCA CSP_SNP_Age_precutEP_rev: GcggccgctACTAGTattaACCGGT TTCTCTCGCTGACGCCGGAAGTGATGTGTGGTTCGC CATGCCGGCttcctccttacTCTAGAagcggccgcG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351002_sequence/1

BBa_K1321344
type: ComponentDefinition
persistent identity: BBa K1321344
display id: BBa_K1321344
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDcex in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:09Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_K1321009
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1321009
sequence: https://synbiohub.org/public/igem/BBa_K1321344_sequence/1

BBa_K1321003
type: Component
persistent identity: component2415107
display id: component2415107
version: 1
top level: BBa_K1321344
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321009
type: Component
persistent identity: component2415104
display id: component2415104
version: 1
top level: BBa_K1321344
access: public
definition: BBa_K1321009

BBa_B0105
type: Component
persistent identity: component2415106
display id: component2415106
version: 1
top level: BBa_K1321344
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415106
display id: annotation2415106
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415106/range2415106/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321344/annotation2415106/range2415106/1
type: Range
persistent identity: range2415106
display id: range2415106
version: 1
direction: direction/reverse
top level: BBa_K1321344
start: 177
end: 182
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415107
display id: annotation2415107
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415107/range2415107/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321344/annotation2415107/range2415107/1
type: Range
persistent identity: range2415107
display id: range2415107
version: 1
direction: direction/reverse
top level: BBa_K1321344
start: 183
end: 509
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415104
display id: annotation2415104
version: 1
top level: BBa_K1321344
location: https://synbiohub.org/public/igem/BBa_K1321344/annotation2415104/range2415104/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321344/annotation2415104/range2415104/1
type: Range
persistent identity: range2415104
display id: range2415104
version: 1
direction: direction/reverse
top level: BBa_K1321344
start: 1
end: 176
orientation: inline

BBa_K1424000
type: ComponentDefinition
persistent identity: BBa K1424000
display id: BBa_K1424000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prefix homologous recombination for Synechocystis ("Left flank")
creator: Jacob Lamb
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1802_
m user id: 0
owner id: 20667
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803. Kunert, A., Hagemann, M. & Erdmann, N. (2000) Construction of promoter probe vectors of Synechocystis sp. PCC 6803 using the light-emitting reporter systems Gfp and LuxAB. J. Microbiol. Methods. 41, 185-194 Angermayr, S. A., Paszota, M. & Hellingwerf, K. J. (2012) Engineering a cyanobacterial cell factory for production of lactic acid. Appl. Environ. Microbiol. 78, 7098-7106
mutable notes: n/a
mutable provenance: This part comes from genomic sequence of Synechocystis sp. PCC 6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence annotation: Synechocystis prefix homologous integration region
sequence: https://synbiohub.org/public/igem/BBa_K1424000_sequence/1

Synechocystis prefix homologous integration region
type: SequenceAnnotation
persistent identity: annotation2400751
display id: annotation2400751
version: 1
top level: BBa_K1424000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1424000/annotation2400751/range2400751/1

https://synbiohub.org/public/igem/BBa_K1424000/annotation2400751/range2400751/1
type: Range
persistent identity: range2400751
display id: range2400751
version: 1
direction: direction/forward
top level: BBa_K1424000
start: 1
end: 556
orientation: inline

BBa_K1420003
type: ComponentDefinition
persistent identity: BBa K1420003
display id: BBa_K1420003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MerP, mercuric transport protein periplasmic component
creator: Stephen C. Heinsch
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T02:53:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: merP is in charge of periplasmic transportation of mercury. This protein has two conserved cysteine residues for specific binding with the mercury ion, Hg(II).
mutable notes: N/A
mutable provenance: Source organism: Serratia marcescens. Plasmid pDU1358 kindly donated by Anne O. Summers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merP
sequence: https://synbiohub.org/public/igem/BBa_K1420003_sequence/1

merP
type: SequenceAnnotation
persistent identity: annotation2407638
display id: annotation2407638
version: 1
top level: BBa_K1420003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420003/annotation2407638/range2407638/1

https://synbiohub.org/public/igem/BBa_K1420003/annotation2407638/range2407638/1
type: Range
persistent identity: range2407638
display id: range2407638
version: 1
direction: direction/forward
top level: BBa_K1420003
start: 1
end: 276
orientation: inline

BBa_K1319004
type: ComponentDefinition
persistent identity: BBa K1319004
display id: BBa_K1319004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEV protease with anti-self cleavage mutation S219V, codon optimized for E. coli
creator: Florian Gohr, Michael Osthege
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a TEV protease in RFC25 that was optimized for expression in E. coli. The part contains the S219V anti-self cleavage mutation.
mutable notes: The part does not contain start or stop codons, because it was designed for RFC25 compability
mutable provenance: The part was codon optimized using the genomic amino acid sequence, designed for RFC25 and mutated S219V for anti-self cleavage properties.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: S219V
sequence annotation: TEV protease
sequence: https://synbiohub.org/public/igem/BBa_K1319004_sequence/1

S219V
type: SequenceAnnotation
persistent identity: annotation2386540
display id: annotation2386540
version: 1
top level: BBa_K1319004
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1319004/annotation2386540/range2386540/1

https://synbiohub.org/public/igem/BBa_K1319004/annotation2386540/range2386540/1
type: Range
persistent identity: range2386540
display id: range2386540
version: 1
direction: direction/forward
top level: BBa_K1319004
start: 655
end: 657
orientation: inline

TEV protease
type: SequenceAnnotation
persistent identity: annotation2386539
display id: annotation2386539
version: 1
top level: BBa_K1319004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1319004/annotation2386539/range2386539/1

https://synbiohub.org/public/igem/BBa_K1319004/annotation2386539/range2386539/1
type: Range
persistent identity: range2386539
display id: range2386539
version: 1
direction: direction/forward
top level: BBa_K1319004
start: 1
end: 726
orientation: inline

BBa_K1333004
type: ComponentDefinition
persistent identity: BBa K1333004
display id: BBa_K1333004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13 bacteriophage g5p with a FLAG tag on C-terminal
creator: Huang Junxiang, Wang Xizi
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 18998
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is coding the M13 bacteriophage g5p with a FLAG tag on C-terminal, which could act as an single strand DNA binding protein and RNA binding protein at the same time. These properties make g5p protein an important regulatory protein for M13 phage. In the life cycle of M13 phage, g5p protein would bind to newly synthesized viral genome ssDNA, resulting in the correct direction to the phage packaging site. Also, g5p protein would repress the expression of g2p protein by binding to the leader sequence of viral mRNA.
mutable notes: Between the g5p and FLAG coding sequencing, there are 9 nucleotides linker sequence coding a 3 amino acid peptide. It is not certain whether this peptide would affect the regular function of g5p protein.
mutable provenance: It is derived from the commercial M13KE vector(New England Biolabs) used in phage display system by PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: g5p
sequence annotation: FLAG
sequence: https://synbiohub.org/public/igem/BBa_K1333004_sequence/1

g5p
type: SequenceAnnotation
persistent identity: annotation2386062
display id: annotation2386062
version: 1
top level: BBa_K1333004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1333004/annotation2386062/range2386062/1

https://synbiohub.org/public/igem/BBa_K1333004/annotation2386062/range2386062/1
type: Range
persistent identity: range2386062
display id: range2386062
version: 1
direction: direction/forward
top level: BBa_K1333004
start: 1
end: 261
orientation: inline

FLAG
type: SequenceAnnotation
persistent identity: annotation2386063
display id: annotation2386063
version: 1
top level: BBa_K1333004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1333004/annotation2386063/range2386063/1

https://synbiohub.org/public/igem/BBa_K1333004/annotation2386063/range2386063/1
type: Range
persistent identity: range2386063
display id: range2386063
version: 1
direction: direction/forward
top level: BBa_K1333004
start: 271
end: 297
orientation: inline

BBa_K1399007
type: ComponentDefinition
persistent identity: BBa K1399007
display id: BBa_K1399007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with SsrA-DAS+2 degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added engineered NYADAS-ssrA degradation tag (see part BBa_M0050). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. SsrA tags encode peptide sequence that and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The NYADAS tag encodes peptide sequence AANDENYNYDAS is reported to have low affinity to ClpX thus its mediated degradation very much depends on the concentration of SspB (ClpX mediator).[1] The two additional residues ???NY??? extends tag between SspB and ClpX binding site, thus preventing clash when both these protein are bound to tag.[3] However, be aware that exact protein degradation rate is influenced by multiple other factors: ClpXP and ClpAP protease concentrations, protein stability, Km of binding to the protease, temperature [4]. ===References=== [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] McGinness, K. E., Baker, T. a & Sauer, R. T. Engineering controllable protein degradation. Mol. Cell 22, 701???7 (2006). [4] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additional residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (DAS in this case) are recognized by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E0040, the tag sequence was obtained from part BBa_M0051.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: NYADAS-ssrA
sequence annotation: GFP (mut3b)
sequence: https://synbiohub.org/public/igem/BBa_K1399007_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2383923
display id: annotation2383923
version: 1
top level: BBa_K1399007
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383923/range2383923/1

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type: Range
persistent identity: range2383923
display id: range2383923
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383926
display id: annotation2383926
version: 1
top level: BBa_K1399007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383926/range2383926/1

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type: Range
persistent identity: range2383926
display id: range2383926
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 754
end: 756
orientation: inline

NYADAS-ssrA
type: SequenceAnnotation
persistent identity: annotation2383925
display id: annotation2383925
version: 1
top level: BBa_K1399007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383925/range2383925/1

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type: Range
persistent identity: range2383925
display id: range2383925
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 715
end: 753
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383927
display id: annotation2383927
version: 1
top level: BBa_K1399007
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383927/range2383927/1

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type: Range
persistent identity: range2383927
display id: range2383927
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 757
end: 759
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383924
display id: annotation2383924
version: 1
top level: BBa_K1399007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399007/annotation2383924/range2383924/1

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type: Range
persistent identity: range2383924
display id: range2383924
version: 1
direction: direction/forward
top level: BBa_K1399007
start: 4
end: 714
orientation: inline

BBa_K1481004
type: ComponentDefinition
persistent identity: BBa K1481004
display id: BBa_K1481004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP with MLRR tag under arabinose promoter
creator: Przemysław Nuc, Melania Nowicka, Weronika Krzynowek, Jakub Bartoszewicz, Katarzyna Rżosińska
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1861_
m user id: 0
owner id: 17561
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: sfGFP under arabinose promoter
mutable notes: -
mutable provenance: Synthetic + genomic E.Coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: O1 signal
sequence annotation: pBAD
sequence annotation: -10 signal
sequence annotation: his-sfGFP
sequence annotation: his-tag
sequence annotation: AraC
sequence annotation: -35 signal
sequence annotation: 5'-UTR
sequence annotation: CRP binding site
sequence annotation: O2 signal
sequence annotation: AraC binding site
sequence: https://synbiohub.org/public/igem/BBa_K1481004_sequence/1

O1 signal
type: SequenceAnnotation
persistent identity: annotation2415102
display id: annotation2415102
version: 1
top level: BBa_K1481004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415102/range2415102/1

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type: Range
persistent identity: range2415102
display id: range2415102
version: 1
direction: direction/forward
top level: BBa_K1481004
start: 909
end: 924
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2415092
display id: annotation2415092
version: 1
top level: BBa_K1481004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415092/range2415092/1

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type: Range
persistent identity: range2415092
display id: range2415092
version: 1
direction: direction/forward
top level: BBa_K1481004
start: 880
end: 1217
orientation: inline

-10 signal
type: SequenceAnnotation
persistent identity: annotation2415167
display id: annotation2415167
version: 1
top level: BBa_K1481004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415167/range2415167/1

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type: Range
persistent identity: range2415167
display id: range2415167
version: 1
direction: direction/forward
top level: BBa_K1481004
start: 1177
end: 1181
orientation: inline

his-sfGFP
type: SequenceAnnotation
persistent identity: annotation2415097
display id: annotation2415097
version: 1
top level: BBa_K1481004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415097/range2415097/1

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type: Range
persistent identity: range2415097
display id: range2415097
version: 1
direction: direction/forward
top level: BBa_K1481004
start: 1218
end: 1967
orientation: inline

his-tag
type: SequenceAnnotation
persistent identity: annotation2415142
display id: annotation2415142
version: 1
top level: BBa_K1481004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415142/range2415142/1

https://synbiohub.org/public/igem/BBa_K1481004/annotation2415142/range2415142/1
type: Range
persistent identity: range2415142
display id: range2415142
version: 1
direction: direction/forward
top level: BBa_K1481004
start: 1230
end: 1247
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2415081
display id: annotation2415081
version: 1
top level: BBa_K1481004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415081/range2415081/1

https://synbiohub.org/public/igem/BBa_K1481004/annotation2415081/range2415081/1
type: Range
persistent identity: range2415081
display id: range2415081
version: 1
direction: direction/reverse
top level: BBa_K1481004
start: 1
end: 879
orientation: inline

-35 signal
type: SequenceAnnotation
persistent identity: annotation2415156
display id: annotation2415156
version: 1
top level: BBa_K1481004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415156/range2415156/1

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type: Range
persistent identity: range2415156
display id: range2415156
version: 1
direction: direction/forward
top level: BBa_K1481004
start: 1153
end: 1157
orientation: inline

5'-UTR
type: SequenceAnnotation
persistent identity: annotation2415117
display id: annotation2415117
version: 1
top level: BBa_K1481004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415117/range2415117/1

https://synbiohub.org/public/igem/BBa_K1481004/annotation2415117/range2415117/1
type: Range
persistent identity: range2415117
display id: range2415117
version: 1
direction: direction/forward
top level: BBa_K1481004
start: 1191
end: 1217
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation2415154
display id: annotation2415154
version: 1
top level: BBa_K1481004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415154/range2415154/1

https://synbiohub.org/public/igem/BBa_K1481004/annotation2415154/range2415154/1
type: Range
persistent identity: range2415154
display id: range2415154
version: 1
direction: direction/forward
top level: BBa_K1481004
start: 1111
end: 1121
orientation: inline

O2 signal
type: SequenceAnnotation
persistent identity: annotation2415115
display id: annotation2415115
version: 1
top level: BBa_K1481004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415115/range2415115/1

https://synbiohub.org/public/igem/BBa_K1481004/annotation2415115/range2415115/1
type: Range
persistent identity: range2415115
display id: range2415115
version: 1
direction: direction/forward
top level: BBa_K1481004
start: 1066
end: 1087
orientation: inline

AraC binding site
type: SequenceAnnotation
persistent identity: annotation2415103
display id: annotation2415103
version: 1
top level: BBa_K1481004
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1481004/annotation2415103/range2415103/1

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type: Range
persistent identity: range2415103
display id: range2415103
version: 1
direction: direction/forward
top level: BBa_K1481004
start: 1113
end: 1155
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0010
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_E1010
sequence annotation: BBa_R0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

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type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_K1351028
type: ComponentDefinition
persistent identity: BBa K1351028
display id: BBa_K1351028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LMU Bacillus RBS collection 1
creator: Florian Riemer
created: 2014-10-05T11:00:00Z
modified: 2015-06-01T08:09:54Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20062
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part was generated in a modified version of RFC25, and has the following prefix and suffix: {||prefix with EcoRI, NotI, XbaI, SD and NgoMIV:|GAATTCGCGGCCGCTTCTAGATGGCCGGC|-|suffix with AgeI, SpeI, NotI and PstI:|ACCGGTTAATACTAGTAGCGGCCGCTGCAG|}Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined.
mutable notes: The LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1351028_sequence/1

BBa_K1497009
type: ComponentDefinition
persistent identity: BBa K1497009
display id: BBa_K1497009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: F3H
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497009_sequence/1

BBa_K1509000
type: ComponentDefinition
persistent identity: BBa K1509000
display id: BBa_K1509000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding for the trans-acting regulator SmtB
creator: Tong Li
created: 2014-09-25T11:00:00Z
modified: 2015-07-23T03:26:04Z
discontinued: false
dominant: false
group u list: _1889_
m user id: 4206
owner id: 24032
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: In 1993, Huckle and co-workers reported the isolation and analysis of the smt locus from Synechococcus sp.. Gene smtB, one of the components of the locus, encodes a transcriptional repressor of gene smtA, with which is required for normal tolerance to Zn2+ and Cd2+. The product of smtB functions by binding to the smt operator-promoter(smtO-P) and dissociating in the presence of Zn2+ due to the classical helix-turn-helix motif similar to many DNA-binding proteins. It can also be genomically rearranged when induced by Cd2+, leading to a deletion within smtB because of a highly iterated palindrome (HIP1), a highly represented octanucleotides(5′-GCGATC-GC-3′) which traverse both borders of the excised element. These two mechanisms both cause the elevated expression of smtA.
mutable notes: According to the mechanisms of smt locus represented above, gene smtB should function with the accompany of smt operator-promoter. Previous cases selected smtA whose product functions by encoding MT-like to sequester metal ions, while our case intended to take advantage of the trigger lies in this locus. By imitating former researches, in which a promoter less lacZ gene was fused upstream of smtA, we respectively splice two selected reporter genes and sequence from smt operator-promoter to smtB, equivalent to replacing smtA with reporter genes, to visualize the effects of metal ions on the locus.
mutable provenance: Metallothioneins(MTs), discovered in horse kidney in 1957 by Margoshes and Vallee are identified as low-molecular weight and sulphydryl rich proteins which bind metal ions in metal-thiolate clusters and whose synthesis increases in response to elevated concentrations of certain metals. In 1988, Olafson and co-workers reported the amino acid sequence of a metal-associated protein isolated from cyanobacterium Synechococcus sp.. With the function of binding metal ions remaining, it only has less than 20% homology in amino acid sequence with known MTs in eukaryotes and thus we call it class II MT or on some occasions MT-like. Owing to the works of Robinson and Huckle, the corresponding locus of Synechococcus PCC7942, smt locus was isolated and analyzed. It includes smtA which encodes the class II MT and a divergently transcribed gene smtB which encodes a trans-acting, autoregulatory repressor that exerts metal ion-inducible negative control over smtA transcription. Between them lies a 100-bp operator-promoter region(smt O-P, BBa_K1509001), which is responsible for the divergent orientation of smtA and smtB.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: smtB
sequence: https://synbiohub.org/public/igem/BBa_K1509000_sequence/1

smtB
type: SequenceAnnotation
persistent identity: annotation2387081
display id: annotation2387081
version: 1
top level: BBa_K1509000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1509000/annotation2387081/range2387081/1

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type: Range
persistent identity: range2387081
display id: range2387081
version: 1
direction: direction/forward
top level: BBa_K1509000
start: 1
end: 301
orientation: inline

BBa_K1321151
type: ComponentDefinition
persistent identity: BBa K1321151
display id: BBa_K1321151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: metallothionein Fmt fused to CBDclos
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321002
component: BBa_K1321012
component: BBa_B0105
sequence annotation: BBa_K1321012
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321151_sequence/1

BBa_K1321002
type: Component
persistent identity: component2415030
display id: component2415030
version: 1
top level: BBa_K1321151
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_K1321012
type: Component
persistent identity: component2415027
display id: component2415027
version: 1
top level: BBa_K1321151
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321012/1

BBa_B0105
type: Component
persistent identity: component2415029
display id: component2415029
version: 1
top level: BBa_K1321151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2415027
display id: annotation2415027
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415027/range2415027/1
component: BBa_K1321012

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type: Range
persistent identity: range2415027
display id: range2415027
version: 1
direction: direction/reverse
top level: BBa_K1321151
start: 1
end: 198
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415030
display id: annotation2415030
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415030/range2415030/1
component: BBa_K1321002

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type: Range
persistent identity: range2415030
display id: range2415030
version: 1
direction: direction/reverse
top level: BBa_K1321151
start: 205
end: 504
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415029
display id: annotation2415029
version: 1
top level: BBa_K1321151
location: https://synbiohub.org/public/igem/BBa_K1321151/annotation2415029/range2415029/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321151/annotation2415029/range2415029/1
type: Range
persistent identity: range2415029
display id: range2415029
version: 1
direction: direction/reverse
top level: BBa_K1321151
start: 199
end: 204
orientation: inline

BBa_K1362004
type: ComponentDefinition
persistent identity: BBa K1362004
display id: BBa_K1362004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A non-circularization construct (with His6)
creator: Silvan Schmitz
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362090
component: BBa_K1362204
sequence annotation: BBa_K1362204
sequence annotation: BBa_K1362090
sequence: https://synbiohub.org/public/igem/BBa_K1362004_sequence/1

BBa_K1362090
type: Component
persistent identity: component2409455
display id: component2409455
version: 1
top level: BBa_K1362004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362204
type: Component
persistent identity: component2409483
display id: component2409483
version: 1
top level: BBa_K1362004
access: public
definition: BBa_K1362204

BBa_K1362204
type: SequenceAnnotation
persistent identity: annotation2409483
display id: annotation2409483
version: 1
top level: BBa_K1362004
location: https://synbiohub.org/public/igem/BBa_K1362004/annotation2409483/range2409483/1
component: BBa_K1362204

https://synbiohub.org/public/igem/BBa_K1362004/annotation2409483/range2409483/1
type: Range
persistent identity: range2409483
display id: range2409483
version: 1
direction: direction/reverse
top level: BBa_K1362004
start: 36
end: 1195
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2409455
display id: annotation2409455
version: 1
top level: BBa_K1362004
location: https://synbiohub.org/public/igem/BBa_K1362004/annotation2409455/range2409455/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362004/annotation2409455/range2409455/1
type: Range
persistent identity: range2409455
display id: range2409455
version: 1
direction: direction/reverse
top level: BBa_K1362004
start: 1
end: 29
orientation: inline

BBa_K1351030
type: ComponentDefinition
persistent identity: BBa K1351030
display id: BBa_K1351030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LMU Bacillus RBS collection 3
creator: Florian Riemer
created: 2014-10-05T11:00:00Z
modified: 2015-06-01T08:10:10Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20062
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: "This part was generated in a modified version of RFC25, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGATGGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined."
mutable notes: The LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1351030_sequence/1

BBa_K1321101
type: ComponentDefinition
persistent identity: BBa K1321101
display id: BBa_K1321101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to CBDclos with T7 promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhytoC+CBD4
mutable notes: PhytoC+CBD4
mutable provenance: PhytoC+CBD4
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321338
component: BBa_K1321005
component: BBa_K1321002
component: BBa_K1319106
component: BBa_B0105
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321338
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321101_sequence/1

BBa_K1321338
type: Component
persistent identity: component2415286
display id: component2415286
version: 1
top level: BBa_K1321101
access: public
definition: BBa_K1321338

BBa_K1321005
type: Component
persistent identity: component2415290
display id: component2415290
version: 1
top level: BBa_K1321101
access: public
definition: BBa_K1321005

BBa_K1321002
type: Component
persistent identity: component2415293
display id: component2415293
version: 1
top level: BBa_K1321101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_K1319106
type: Component
persistent identity: component2415288
display id: component2415288
version: 1
top level: BBa_K1321101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_B0105
type: Component
persistent identity: component2415292
display id: component2415292
version: 1
top level: BBa_K1321101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415288
display id: annotation2415288
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415288/range2415288/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415288/range2415288/1
type: Range
persistent identity: range2415288
display id: range2415288
version: 1
direction: direction/reverse
top level: BBa_K1321101
start: 44
end: 58
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2415290
display id: annotation2415290
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415290/range2415290/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415290/range2415290/1
type: Range
persistent identity: range2415290
display id: range2415290
version: 1
direction: direction/reverse
top level: BBa_K1321101
start: 59
end: 181
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415286
display id: annotation2415286
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415286/range2415286/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415286/range2415286/1
type: Range
persistent identity: range2415286
display id: range2415286
version: 1
direction: direction/reverse
top level: BBa_K1321101
start: 1
end: 43
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415292
display id: annotation2415292
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415292/range2415292/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415292/range2415292/1
type: Range
persistent identity: range2415292
display id: range2415292
version: 1
direction: direction/reverse
top level: BBa_K1321101
start: 182
end: 187
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415293
display id: annotation2415293
version: 1
top level: BBa_K1321101
location: https://synbiohub.org/public/igem/BBa_K1321101/annotation2415293/range2415293/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321101/annotation2415293/range2415293/1
type: Range
persistent identity: range2415293
display id: range2415293
version: 1
direction: direction/reverse
top level: BBa_K1321101
start: 188
end: 487
orientation: inline

BBa_K1357009
type: ComponentDefinition
persistent identity: BBa K1357009
display id: BBa_K1357009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amilCP Reporter (RBS+Coding+Term)
creator: Matthew Tucker
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
group u list: _1733_
m user id: 0
owner id: 16772
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Show functionality of gene circuit
mutable notes: None
mutable provenance: jellyfish
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_B0034
component: BBa_K592009
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_K592009
sequence: https://synbiohub.org/public/igem/BBa_K1357009_sequence/1

BBa_B0015
type: Component
persistent identity: component2408711
display id: component2408711
version: 1
top level: BBa_K1357009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2408702
display id: component2408702
version: 1
top level: BBa_K1357009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K592009
type: Component
persistent identity: component2408704
display id: component2408704
version: 1
top level: BBa_K1357009
access: public
definition: https://synbiohub.org/public/igem/BBa_K592009/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2408702
display id: annotation2408702
version: 1
top level: BBa_K1357009
location: https://synbiohub.org/public/igem/BBa_K1357009/annotation2408702/range2408702/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1357009/annotation2408702/range2408702/1
type: Range
persistent identity: range2408702
display id: range2408702
version: 1
direction: direction/reverse
top level: BBa_K1357009
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2408711
display id: annotation2408711
version: 1
top level: BBa_K1357009
location: https://synbiohub.org/public/igem/BBa_K1357009/annotation2408711/range2408711/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1357009/annotation2408711/range2408711/1
type: Range
persistent identity: range2408711
display id: range2408711
version: 1
direction: direction/reverse
top level: BBa_K1357009
start: 696
end: 824
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2408704
display id: annotation2408704
version: 1
top level: BBa_K1357009
location: https://synbiohub.org/public/igem/BBa_K1357009/annotation2408704/range2408704/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K1357009/annotation2408704/range2408704/1
type: Range
persistent identity: range2408704
display id: range2408704
version: 1
direction: direction/reverse
top level: BBa_K1357009
start: 19
end: 687
orientation: inline

BBa_K1403013
type: ComponentDefinition
persistent identity: BBa K1403013
display id: BBa_K1403013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Geosmin expression cassette
creator: Paris Bettencourt 2014
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1781_
m user id: 0
owner id: 8369
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Germacradienol/germacrene D synthase CDS.
mutable notes: Synthetic RBS, IDT gBlock.
mutable provenance: gBlock
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: GDS
sequence annotation: 6-His
sequence annotation: sRBS
sequence annotation: BBa_J23108
sequence annotation: stop
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K1403013_sequence/1

GDS
type: SequenceAnnotation
persistent identity: annotation2396610
display id: annotation2396610
version: 1
top level: BBa_K1403013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1403013/annotation2396610/range2396610/1

https://synbiohub.org/public/igem/BBa_K1403013/annotation2396610/range2396610/1
type: Range
persistent identity: range2396610
display id: range2396610
version: 1
direction: direction/forward
top level: BBa_K1403013
start: 77
end: 1174
orientation: inline

6-His
type: SequenceAnnotation
persistent identity: annotation2396611
display id: annotation2396611
version: 1
top level: BBa_K1403013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1403013/annotation2396611/range2396611/1

https://synbiohub.org/public/igem/BBa_K1403013/annotation2396611/range2396611/1
type: Range
persistent identity: range2396611
display id: range2396611
version: 1
direction: direction/reverse
top level: BBa_K1403013
start: 1175
end: 1192
orientation: inline

sRBS
type: SequenceAnnotation
persistent identity: annotation2396609
display id: annotation2396609
version: 1
top level: BBa_K1403013
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1403013/annotation2396609/range2396609/1

https://synbiohub.org/public/igem/BBa_K1403013/annotation2396609/range2396609/1
type: Range
persistent identity: range2396609
display id: range2396609
version: 1
direction: direction/forward
top level: BBa_K1403013
start: 42
end: 76
orientation: inline

BBa_J23108
type: SequenceAnnotation
persistent identity: annotation2396608
display id: annotation2396608
version: 1
top level: BBa_K1403013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1403013/annotation2396608/range2396608/1

https://synbiohub.org/public/igem/BBa_K1403013/annotation2396608/range2396608/1
type: Range
persistent identity: range2396608
display id: range2396608
version: 1
direction: direction/forward
top level: BBa_K1403013
start: 1
end: 35
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2427975
display id: annotation2427975
version: 1
top level: BBa_K1403013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1403013/annotation2427975/range2427975/1

https://synbiohub.org/public/igem/BBa_K1403013/annotation2427975/range2427975/1
type: Range
persistent identity: range2427975
display id: range2427975
version: 1
direction: direction/forward
top level: BBa_K1403013
start: 1193
end: 1195
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation2427974
display id: annotation2427974
version: 1
top level: BBa_K1403013
role: feature/scar
location: https://synbiohub.org/public/igem/BBa_K1403013/annotation2427974/range2427974/1

https://synbiohub.org/public/igem/BBa_K1403013/annotation2427974/range2427974/1
type: Range
persistent identity: range2427974
display id: range2427974
version: 1
direction: direction/forward
top level: BBa_K1403013
start: 36
end: 41
orientation: inline

BBa_K1491017
type: ComponentDefinition
persistent identity: BBa K1491017
display id: BBa_K1491017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Codon Optimized Supernova
creator: Danielle Peters
created: 2014-08-03T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Supernova is the monomeric version of KillerRed (BBa_K1184000). It has been codon optimized for E. coli.
mutable notes: Monomeric version of KillerRed
mutable provenance: The original KillerRed is BBa_K11840000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x His Tag
sequence: https://synbiohub.org/public/igem/BBa_K1491017_sequence/1

6x His Tag
type: SequenceAnnotation
persistent identity: annotation2380356
display id: annotation2380356
version: 1
top level: BBa_K1491017
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1491017/annotation2380356/range2380356/1

https://synbiohub.org/public/igem/BBa_K1491017/annotation2380356/range2380356/1
type: Range
persistent identity: range2380356
display id: range2380356
version: 1
direction: direction/forward
top level: BBa_K1491017
start: 718
end: 735
orientation: inline

BBa_K1333305
type: ComponentDefinition
persistent identity: BBa K1333305
display id: BBa_K1333305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13453-FourU
creator: Runwen Yao
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15873
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a combination of a pBAD promoter(BBa_I13453), RNA thermometer(BBa_K115002）
mutable provenance: The source of the part will be its components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K115002
component: BBa_I13453
sequence annotation: BBa_K115002
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1333305_sequence/1

BBa_K115002
type: Component
persistent identity: component2385944
display id: component2385944
version: 1
top level: BBa_K1333305
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_I13453
type: Component
persistent identity: component2385939
display id: component2385939
version: 1
top level: BBa_K1333305
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2385944
display id: annotation2385944
version: 1
top level: BBa_K1333305
location: https://synbiohub.org/public/igem/BBa_K1333305/annotation2385944/range2385944/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K1333305/annotation2385944/range2385944/1
type: Range
persistent identity: range2385944
display id: range2385944
version: 1
direction: direction/reverse
top level: BBa_K1333305
start: 139
end: 190
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2385939
display id: annotation2385939
version: 1
top level: BBa_K1333305
location: https://synbiohub.org/public/igem/BBa_K1333305/annotation2385939/range2385939/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1333305/annotation2385939/range2385939/1
type: Range
persistent identity: range2385939
display id: range2385939
version: 1
direction: direction/reverse
top level: BBa_K1333305
start: 1
end: 130
orientation: inline

BBa_K1362005
type: ComponentDefinition
persistent identity: BBa K1362005
display id: BBa_K1362005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A non-circularization construct (with Smt3 and His6)
creator: Silvan Schmitz
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362205
component: BBa_K1362090
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362205
sequence: https://synbiohub.org/public/igem/BBa_K1362005_sequence/1

BBa_K1362205
type: Component
persistent identity: component2409518
display id: component2409518
version: 1
top level: BBa_K1362005
access: public
definition: BBa_K1362205

BBa_K1362090
type: Component
persistent identity: component2409486
display id: component2409486
version: 1
top level: BBa_K1362005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2409486
display id: annotation2409486
version: 1
top level: BBa_K1362005
location: https://synbiohub.org/public/igem/BBa_K1362005/annotation2409486/range2409486/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362005/annotation2409486/range2409486/1
type: Range
persistent identity: range2409486
display id: range2409486
version: 1
direction: direction/reverse
top level: BBa_K1362005
start: 1
end: 29
orientation: inline

BBa_K1362205
type: SequenceAnnotation
persistent identity: annotation2409518
display id: annotation2409518
version: 1
top level: BBa_K1362005
location: https://synbiohub.org/public/igem/BBa_K1362005/annotation2409518/range2409518/1
component: BBa_K1362205

https://synbiohub.org/public/igem/BBa_K1362005/annotation2409518/range2409518/1
type: Range
persistent identity: range2409518
display id: range2409518
version: 1
direction: direction/reverse
top level: BBa_K1362005
start: 36
end: 1522
orientation: inline

BBa_K1351031
type: ComponentDefinition
persistent identity: BBa K1351031
display id: BBa_K1351031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LMU Bacillus RBS collection 4
creator: Florian Riemer
created: 2014-10-05T11:00:00Z
modified: 2015-06-01T08:10:17Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20062
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: "This part was generated in a modified version of RFC25, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGATGGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined."
mutable notes: The LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1351031_sequence/1

BBa_K1508002
type: ComponentDefinition
persistent identity: BBa K1508002
display id: BBa_K1508002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IIA(Glc) - E.coli glucose-specific phosphotransferase enzyme IIA
creator: Fahmi Dwilaksono, Indah Nurulita, Rian Adha Ardinata, Mochammad Isro Alfajri, Arief Budi Witarto
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:47Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1888_
m user id: 0
owner id: 16581
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Phosphotransferase system (PTS) is a major carbohydrate active -transport system, which is executed by a cascade of protein rally. For glucose uptake, IIA(Glc) is the only enzyme specific to glucose transport. We measure the concentration of glucose in the medium using catabolite repression mechanism toward lacI promoter activity upstream of a reporter gene, mRFP. We expect by over-expressing IIA(Glc) in E. coli, we will obtain different range of glucose standard curve in our measurement system.
mutable notes: The gene coding for IIA(Glc) from E.coli - crr - was isolated by colony PCR using forward primer which contains with restriction sites of EcoRI and XbaI and reverse primers which contains restriction site for SpeI and a double stop codon.
mutable provenance: Escherichia coli DH5alpha genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: IIA(Glc) coding region
sequence annotation: TAA TAA stop codon
sequence annotation: ATG start codon
sequence: https://synbiohub.org/public/igem/BBa_K1508002_sequence/1

IIA(Glc) coding region
type: SequenceAnnotation
persistent identity: annotation2407417
display id: annotation2407417
version: 1
top level: BBa_K1508002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1508002/annotation2407417/range2407417/1

https://synbiohub.org/public/igem/BBa_K1508002/annotation2407417/range2407417/1
type: Range
persistent identity: range2407417
display id: range2407417
version: 1
direction: direction/forward
top level: BBa_K1508002
start: 1
end: 513
orientation: inline

TAA TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2407420
display id: annotation2407420
version: 1
top level: BBa_K1508002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1508002/annotation2407420/range2407420/1

https://synbiohub.org/public/igem/BBa_K1508002/annotation2407420/range2407420/1
type: Range
persistent identity: range2407420
display id: range2407420
version: 1
direction: direction/forward
top level: BBa_K1508002
start: 508
end: 513
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2407415
display id: annotation2407415
version: 1
top level: BBa_K1508002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1508002/annotation2407415/range2407415/1

https://synbiohub.org/public/igem/BBa_K1508002/annotation2407415/range2407415/1
type: Range
persistent identity: range2407415
display id: range2407415
version: 1
direction: direction/forward
top level: BBa_K1508002
start: 1
end: 3
orientation: inline

BBa_K1399005
type: ComponentDefinition
persistent identity: BBa K1399005
display id: BBa_K1399005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with AAV-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added AAV-ssrA degradation tag (part BBa_I11012). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYAAAV and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The AAV tag is reported to lead to reasonably fast protein degradation, degrading GFP with rate -0.012 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E0040, tag sequence was obtained from part I11012, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: GFP (mut3b)
sequence annotation: AAV-ssrA tag
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399005_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383911
display id: annotation2383911
version: 1
top level: BBa_K1399005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383911/range2383911/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383911/range2383911/1
type: Range
persistent identity: range2383911
display id: range2383911
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 748
end: 750
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383909
display id: annotation2383909
version: 1
top level: BBa_K1399005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383909/range2383909/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383909/range2383909/1
type: Range
persistent identity: range2383909
display id: range2383909
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 4
end: 714
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383910
display id: annotation2383910
version: 1
top level: BBa_K1399005
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383910/range2383910/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383910/range2383910/1
type: Range
persistent identity: range2383910
display id: range2383910
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 715
end: 747
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383912
display id: annotation2383912
version: 1
top level: BBa_K1399005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383912/range2383912/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383912/range2383912/1
type: Range
persistent identity: range2383912
display id: range2383912
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 751
end: 753
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383908
display id: annotation2383908
version: 1
top level: BBa_K1399005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399005/annotation2383908/range2383908/1

https://synbiohub.org/public/igem/BBa_K1399005/annotation2383908/range2383908/1
type: Range
persistent identity: range2383908
display id: range2383908
version: 1
direction: direction/forward
top level: BBa_K1399005
start: 1
end: 3
orientation: inline

BBa_K1351013
type: ComponentDefinition
persistent identity: BBa K1351013
display id: BBa_K1351013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spaS Subtilin (antimicrobial peptide)
creator: Carolin Kobras
created: 2014-10-06T11:00:00Z
modified: 2016-01-27T02:42:29Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20398
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Subtilin is a lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. This BioBrick differs in its overhangs from BBa_K1351012.
mutable notes: -
mutable provenance: Bacillus subtilis ATCC6633
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351013_sequence/1

ONBY RS
type: ComponentDefinition
persistent identity: BBa K1416000
display id: BBa_K1416000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The tRNA synthetase/tRNA needed for incorporating o-(2-nitrobenzyl)-L-tyrosine (ONBY) at a UAG codon
creator: Jordan Monk
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1794_
m user id: 0
owner id: 17383
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The part includes the gene coding for the Methanocaldococcus jannaschii synthetase mutated to charge the orthogonal tRNA (also in the part) with the non-canonical amino acid o-nitrobenzyl tyrosine (ONBY) along with the proper promoters and terminator . This part encodes for the incorporation of ONBY during translation at AUG amber stop codons in a cell with Release Factor 1 (RF-1) knocked out such as "Amberless" E. coli or "RF-0".
mutable provenance: Evolved from the Methanocaldococcus janaschii tyrosyl synthetase and tRNA by the Schulz group. Specific mutations sourced from Deiters, A., Groff, D., Ryu, Y., Xie, J. and Schultz, P. G. (2006), A Genetically Encoded Photocaged Tyrosine. Angew. Chem. Int. Ed., 45: 2728???2731.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tRNA
sequence annotation: terminator
sequence annotation: ONBYRS promoter
sequence annotation: tRNA promoter
sequence annotation: ONBYRS
sequence: https://synbiohub.org/public/igem/BBa_K1416000_sequence/1

tRNA
type: SequenceAnnotation
persistent identity: annotation2426654
display id: annotation2426654
version: 1
top level: ONBY RS
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1416000/annotation2426654/range2426654/1

https://synbiohub.org/public/igem/BBa_K1416000/annotation2426654/range2426654/1
type: Range
persistent identity: range2426654
display id: range2426654
version: 1
direction: direction/forward
top level: ONBY RS
start: 1396
end: 1471
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2426655
display id: annotation2426655
version: 1
top level: ONBY RS
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1416000/annotation2426655/range2426655/1

https://synbiohub.org/public/igem/BBa_K1416000/annotation2426655/range2426655/1
type: Range
persistent identity: range2426655
display id: range2426655
version: 1
direction: direction/reverse
top level: ONBY RS
start: 1497
end: 1526
orientation: inline

ONBYRS promoter
type: SequenceAnnotation
persistent identity: annotation2426651
display id: annotation2426651
version: 1
top level: ONBY RS
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1416000/annotation2426651/range2426651/1

https://synbiohub.org/public/igem/BBa_K1416000/annotation2426651/range2426651/1
type: Range
persistent identity: range2426651
display id: range2426651
version: 1
direction: direction/forward
top level: ONBY RS
start: 72
end: 219
orientation: inline

tRNA promoter
type: SequenceAnnotation
persistent identity: annotation2426653
display id: annotation2426653
version: 1
top level: ONBY RS
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1416000/annotation2426653/range2426653/1

https://synbiohub.org/public/igem/BBa_K1416000/annotation2426653/range2426653/1
type: Range
persistent identity: range2426653
display id: range2426653
version: 1
direction: direction/forward
top level: ONBY RS
start: 1330
end: 1379
orientation: inline

ONBYRS
type: SequenceAnnotation
persistent identity: annotation2426652
display id: annotation2426652
version: 1
top level: ONBY RS
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1416000/annotation2426652/range2426652/1

https://synbiohub.org/public/igem/BBa_K1416000/annotation2426652/range2426652/1
type: Range
persistent identity: range2426652
display id: range2426652
version: 1
direction: direction/forward
top level: ONBY RS
start: 233
end: 1153
orientation: inline

BBa_K1321163
type: ComponentDefinition
persistent identity: BBa K1321163
display id: BBa_K1321163
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP driven by T7 promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321009
component: BBa_K1321338
sequence annotation: BBa_K1321009
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321163_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415076
display id: component2415076
version: 1
top level: BBa_K1321163
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321009
type: Component
persistent identity: component2415077
display id: component2415077
version: 1
top level: BBa_K1321163
access: public
definition: BBa_K1321009

BBa_K1321338
type: Component
persistent identity: component2415074
display id: component2415074
version: 1
top level: BBa_K1321163
access: public
definition: BBa_K1321338

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415077
display id: annotation2415077
version: 1
top level: BBa_K1321163
location: https://synbiohub.org/public/igem/BBa_K1321163/annotation2415077/range2415077/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321163/annotation2415077/range2415077/1
type: Range
persistent identity: range2415077
display id: range2415077
version: 1
direction: direction/reverse
top level: BBa_K1321163
start: 59
end: 234
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415076
display id: annotation2415076
version: 1
top level: BBa_K1321163
location: https://synbiohub.org/public/igem/BBa_K1321163/annotation2415076/range2415076/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321163/annotation2415076/range2415076/1
type: Range
persistent identity: range2415076
display id: range2415076
version: 1
direction: direction/reverse
top level: BBa_K1321163
start: 44
end: 58
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415074
display id: annotation2415074
version: 1
top level: BBa_K1321163
location: https://synbiohub.org/public/igem/BBa_K1321163/annotation2415074/range2415074/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321163/annotation2415074/range2415074/1
type: Range
persistent identity: range2415074
display id: range2415074
version: 1
direction: direction/reverse
top level: BBa_K1321163
start: 1
end: 43
orientation: inline

BBa_K1529321
type: ComponentDefinition
persistent identity: BBa K1529321
display id: BBa_K1529321
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prhl(RR)_GFP
creator: Kohdai Hibi
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:10:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1911_
m user id: 0
owner id: 22688
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1529320
component: BBa_J54103
sequence annotation: BBa_K1529320
sequence annotation: BBa_J54103
sequence: https://synbiohub.org/public/igem/BBa_K1529321_sequence/1

BBa_K1529320
type: Component
persistent identity: component2417658
display id: component2417658
version: 1
top level: BBa_K1529321
access: public
definition: https://synbiohub.org/public/igem/BBa_K1529320/1

BBa_J54103
type: Component
persistent identity: component2417673
display id: component2417673
version: 1
top level: BBa_K1529321
access: public
definition: https://synbiohub.org/public/igem/BBa_J54103/1

BBa_K1529320
type: SequenceAnnotation
persistent identity: annotation2417658
display id: annotation2417658
version: 1
top level: BBa_K1529321
location: https://synbiohub.org/public/igem/BBa_K1529321/annotation2417658/range2417658/1
component: BBa_K1529320

https://synbiohub.org/public/igem/BBa_K1529321/annotation2417658/range2417658/1
type: Range
persistent identity: range2417658
display id: range2417658
version: 1
direction: direction/reverse
top level: BBa_K1529321
start: 1
end: 55
orientation: inline

BBa_J54103
type: SequenceAnnotation
persistent identity: annotation2417673
display id: annotation2417673
version: 1
top level: BBa_K1529321
location: https://synbiohub.org/public/igem/BBa_K1529321/annotation2417673/range2417673/1
component: BBa_J54103

https://synbiohub.org/public/igem/BBa_K1529321/annotation2417673/range2417673/1
type: Range
persistent identity: range2417673
display id: range2417673
version: 1
direction: direction/reverse
top level: BBa_K1529321
start: 64
end: 990
orientation: inline

REACh2
type: ComponentDefinition
persistent identity: BBa K1319002
display id: BBa_K1319002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFC[25]-compatible dark quencher based on K1319000 (E0030)
creator: Michael Osthege, Florian Gohr
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP). Three mutations were introduced that eliminated fluorescence: * L90I * Y145W * H148R
mutable notes: Point mutations were introduced, starting from K1319000 (RFC25-YFP) as the template.
mutable provenance: The part is derived from K1319000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: REACh2
sequence: https://synbiohub.org/public/igem/BBa_K1319002_sequence/1

REACh2
type: SequenceAnnotation
persistent identity: annotation2390763
display id: annotation2390763
version: 1
top level: REACh2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1319002/annotation2390763/range2390763/1

https://synbiohub.org/public/igem/BBa_K1319002/annotation2390763/range2390763/1
type: Range
persistent identity: range2390763
display id: range2390763
version: 1
direction: direction/forward
top level: REACh2
start: 1
end: 711
orientation: inline

BBa_K1321352
type: ComponentDefinition
persistent identity: BBa K1321352
display id: BBa_K1321352
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDcex driven by T7
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:31:54Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321344
component: BBa_K1321338
sequence annotation: BBa_K1321344
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321352_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415430
display id: component2415430
version: 1
top level: BBa_K1321352
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321344
type: Component
persistent identity: component2415435
display id: component2415435
version: 1
top level: BBa_K1321352
access: public
definition: BBa_K1321344

BBa_K1321338
type: Component
persistent identity: component2415428
display id: component2415428
version: 1
top level: BBa_K1321352
access: public
definition: BBa_K1321338

BBa_K1321344
type: SequenceAnnotation
persistent identity: annotation2415435
display id: annotation2415435
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415435/range2415435/1
component: BBa_K1321344

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415435/range2415435/1
type: Range
persistent identity: range2415435
display id: range2415435
version: 1
direction: direction/reverse
top level: BBa_K1321352
start: 59
end: 567
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415430
display id: annotation2415430
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415430/range2415430/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415430/range2415430/1
type: Range
persistent identity: range2415430
display id: range2415430
version: 1
direction: direction/reverse
top level: BBa_K1321352
start: 44
end: 58
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415428
display id: annotation2415428
version: 1
top level: BBa_K1321352
location: https://synbiohub.org/public/igem/BBa_K1321352/annotation2415428/range2415428/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321352/annotation2415428/range2415428/1
type: Range
persistent identity: range2415428
display id: range2415428
version: 1
direction: direction/reverse
top level: BBa_K1321352
start: 1
end: 43
orientation: inline

Intein
type: ComponentDefinition
persistent identity: BBa K1483003
display id: BBa_K1483003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ssp GyrB Split Intein
creator: Nikolas Layer, Philipp H. O. Mayer and Philip Roessler
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1863_
m user id: 0
owner id: 21259
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: N-Intein part of the ssp GyrB split intein. Can be used in conjunction with the 6 amino acid long C-Intein to N-terminally immobilize a peptide onto a matrix. For immobilisation, parts need to be fused to the C-terminus of the N-intein. The N-terminus of the C-Intein is fused the matrix. The split intein recombines and splices itself out, thereby specificially immobilising the N-terminus of the peptide to the matrix. The sequence of the corresponding C-Intein is: H2N-GVPVHN-COOH (was aquired by peptide synthesis and is thus not part of the registry) This part can also be used in order to create fusion proteins, by attaching the C-Intein to a proein instead of the matrix. Part in RFC25 standard.
mutable notes: Part was designed in RFC25, since it is specifically used for fusion of peptides
mutable provenance: Intein of DNA grase subunit B of the cyanobacterium Synechocystis species, strain PCC6804. Sequence optimized for codon usage in E. coli K12 and synthesised.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Intein
sequence: https://synbiohub.org/public/igem/BBa_K1483003_sequence/1

Intein
type: SequenceAnnotation
persistent identity: annotation2396716
display id: annotation2396716
version: 1
top level: Intein
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1483003/annotation2396716/range2396716/1

https://synbiohub.org/public/igem/BBa_K1483003/annotation2396716/range2396716/1
type: Range
persistent identity: range2396716
display id: range2396716
version: 1
direction: direction/forward
top level: Intein
start: 1
end: 450
orientation: inline

BBa_K1319020
type: ComponentDefinition
persistent identity: BBa K1319020
display id: BBa_K1319020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Translational unit of mRFP-galectin3-his
creator: Michael Osthege
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:09:51Z
discontinued: false
dominant: false
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a translational unit of a mRFP-galectin-3-his (B0032.E1010.K1319003.K1319016.B0015)
mutable notes: The stop of K1319003 was removed and substituted by a His6-tag.
mutable provenance: The part is a combination of other BioBrick parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_K1319009
sequence annotation: BBa_B0034
sequence annotation: BBa_K1319009
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1319020_sequence/1

BBa_B0034
type: Component
persistent identity: component2392190
display id: component2392190
version: 1
top level: BBa_K1319020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2392198
display id: component2392198
version: 1
top level: BBa_K1319020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_K1319009
type: Component
persistent identity: component2392191
display id: component2392191
version: 1
top level: BBa_K1319020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319009/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392190
display id: annotation2392190
version: 1
top level: BBa_K1319020
location: https://synbiohub.org/public/igem/BBa_K1319020/annotation2392190/range2392190/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1319020/annotation2392190/range2392190/1
type: Range
persistent identity: range2392190
display id: range2392190
version: 1
direction: direction/reverse
top level: BBa_K1319020
start: 1
end: 12
orientation: inline

BBa_K1319009
type: SequenceAnnotation
persistent identity: annotation2392191
display id: annotation2392191
version: 1
top level: BBa_K1319020
location: https://synbiohub.org/public/igem/BBa_K1319020/annotation2392191/range2392191/1
component: BBa_K1319009

https://synbiohub.org/public/igem/BBa_K1319020/annotation2392191/range2392191/1
type: Range
persistent identity: range2392191
display id: range2392191
version: 1
direction: direction/reverse
top level: BBa_K1319020
start: 19
end: 1476
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2392198
display id: annotation2392198
version: 1
top level: BBa_K1319020
location: https://synbiohub.org/public/igem/BBa_K1319020/annotation2392198/range2392198/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1319020/annotation2392198/range2392198/1
type: Range
persistent identity: range2392198
display id: range2392198
version: 1
direction: direction/reverse
top level: BBa_K1319020
start: 1485
end: 1613
orientation: inline

1Gate-NpuD
type: ComponentDefinition
persistent identity: BBa K1362100
display id: BBa_K1362100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + NpuDnaE N-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362422
component: BBa_K1362417
component: BBa_K1362437
component: BBa_K1362400
component: BBa_K1362090
component: BBa_K1362430
component: BBa_G0000
component: BBa_K1362414
component: BBa_K1362421
component: BBa_K1362415
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362400
sequence: https://synbiohub.org/public/igem/BBa_K1362100_sequence/1

BBa_K1362422
type: Component
persistent identity: component2410043
display id: component2410043
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362417
type: Component
persistent identity: component2410048
display id: component2410048
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362437
type: Component
persistent identity: component2410055
display id: component2410055
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362400
type: Component
persistent identity: component2410045
display id: component2410045
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362400/1

BBa_K1362090
type: Component
persistent identity: component2410020
display id: component2410020
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362430
type: Component
persistent identity: component2410051
display id: component2410051
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_G0000
type: Component
persistent identity: component2410021
display id: component2410021
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362414
type: Component
persistent identity: component2410022
display id: component2410022
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362421
type: Component
persistent identity: component2410053
display id: component2410053
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362415
type: Component
persistent identity: component2410044
display id: component2410044
version: 1
top level: 1Gate-NpuD
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410021
display id: annotation2410021
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410021/range2410021/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410021/range2410021/1
type: Range
persistent identity: range2410021
display id: range2410021
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 30
end: 35
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410022
display id: annotation2410022
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410022/range2410022/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410022/range2410022/1
type: Range
persistent identity: range2410022
display id: range2410022
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 36
end: 38
orientation: inline

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410048
display id: annotation2410048
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410048/range2410048/1
component: BBa_K1362417

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410048/range2410048/1
type: Range
persistent identity: range2410048
display id: range2410048
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1428
end: 1433
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410053
display id: annotation2410053
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410053/range2410053/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410053/range2410053/1
type: Range
persistent identity: range2410053
display id: range2410053
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1458
end: 1461
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410043
display id: annotation2410043
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410043/range2410043/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410043/range2410043/1
type: Range
persistent identity: range2410043
display id: range2410043
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 39
end: 1121
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410051
display id: annotation2410051
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410051/range2410051/1
component: BBa_K1362430

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410051/range2410051/1
type: Range
persistent identity: range2410051
display id: range2410051
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1434
end: 1457
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410055
display id: annotation2410055
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410055/range2410055/1
component: BBa_K1362437

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410055/range2410055/1
type: Range
persistent identity: range2410055
display id: range2410055
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1462
end: 1464
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410020
display id: annotation2410020
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410020/range2410020/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410020/range2410020/1
type: Range
persistent identity: range2410020
display id: range2410020
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1
end: 29
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2410044
display id: annotation2410044
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410044/range2410044/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410044/range2410044/1
type: Range
persistent identity: range2410044
display id: range2410044
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1122
end: 1125
orientation: inline

BBa_K1362400
type: SequenceAnnotation
persistent identity: annotation2410045
display id: annotation2410045
version: 1
top level: 1Gate-NpuD
location: https://synbiohub.org/public/igem/BBa_K1362100/annotation2410045/range2410045/1
component: BBa_K1362400

https://synbiohub.org/public/igem/BBa_K1362100/annotation2410045/range2410045/1
type: Range
persistent identity: range2410045
display id: range2410045
version: 1
direction: direction/reverse
top level: 1Gate-NpuD
start: 1126
end: 1427
orientation: inline

BBa_K1499200
type: ComponentDefinition
persistent identity: BBa K1499200
display id: BBa_K1499200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Deinococcus radiodurans UV DNA damage endonuclease - uvsE
creator: Raman Nelakanti
created: 2014-08-21T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1879_
m user id: 0
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: TODO
mutable notes: TODO - mention the different starting points
mutable provenance: Deinococcus radiodurans R1 genomic DNA from Chromosome 1
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG Start Codon
sequence annotation: TAA Stop
sequence annotation: uvsE
sequence: https://synbiohub.org/public/igem/BBa_K1499200_sequence/1

ATG Start Codon
type: SequenceAnnotation
persistent identity: annotation2381266
display id: annotation2381266
version: 1
top level: BBa_K1499200
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499200/annotation2381266/range2381266/1

https://synbiohub.org/public/igem/BBa_K1499200/annotation2381266/range2381266/1
type: Range
persistent identity: range2381266
display id: range2381266
version: 1
direction: direction/forward
top level: BBa_K1499200
start: 1
end: 3
orientation: inline

TAA Stop
type: SequenceAnnotation
persistent identity: annotation2381267
display id: annotation2381267
version: 1
top level: BBa_K1499200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1499200/annotation2381267/range2381267/1

https://synbiohub.org/public/igem/BBa_K1499200/annotation2381267/range2381267/1
type: Range
persistent identity: range2381267
display id: range2381267
version: 1
direction: direction/forward
top level: BBa_K1499200
start: 916
end: 918
orientation: inline

uvsE
type: SequenceAnnotation
persistent identity: annotation2427734
display id: annotation2427734
version: 1
top level: BBa_K1499200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1499200/annotation2427734/range2427734/1

https://synbiohub.org/public/igem/BBa_K1499200/annotation2427734/range2427734/1
type: Range
persistent identity: range2427734
display id: range2427734
version: 1
direction: direction/forward
top level: BBa_K1499200
start: 1
end: 918
orientation: inline

BBa_K1402010
type: ComponentDefinition
persistent identity: BBa K1402010
display id: BBa_K1402010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Promoter
creator: Chen Xi
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1780_
m user id: 0
owner id: 16730
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is an inverting regulator sensitive to LacI (BBa_C0010, BBa_C0012, etc.) The T7-lac promoter is a hybrid regulatory region consisting of the T7 promoter of T7 phage next to LacO. The hybrid design allows for strong promotion that can nevertheless be:  repressed by LacI  induced by lac or IPTG, showing a high expression level.  CAP protein will not influence the expression. Strong promoter. Only E.Coli BL21(DE3) can use this part.
mutable notes: no
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1402010_sequence/1

BBa_K1333312
type: ComponentDefinition
persistent identity: BBa K1333312
display id: BBa_K1333312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13458-I13453-RBS-G3P(M13)
creator: Wei Tang
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1708_
m user id: 0
owner id: 15896
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: expression PⅢ, which is from M13 phage genome, with the regulation of araBAD promoter.
mutable notes: For the rescure experiment.
mutable provenance: PⅢ is from M13 phage genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I13453
component: BBa_I13458
component: BBa_K1333002
component: BBa_B0034
sequence annotation: BBa_I13458
sequence annotation: BBa_K1333002
sequence annotation: BBa_B0034
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1333312_sequence/1

BBa_I13453
type: Component
persistent identity: component2386081
display id: component2386081
version: 1
top level: BBa_K1333312
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_I13458
type: Component
persistent identity: component2386080
display id: component2386080
version: 1
top level: BBa_K1333312
access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_K1333002
type: Component
persistent identity: component2386087
display id: component2386087
version: 1
top level: BBa_K1333312
access: public
definition: https://synbiohub.org/public/igem/BBa_K1333002/1

BBa_B0034
type: Component
persistent identity: component2386083
display id: component2386083
version: 1
top level: BBa_K1333312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation2386080
display id: annotation2386080
version: 1
top level: BBa_K1333312
location: https://synbiohub.org/public/igem/BBa_K1333312/annotation2386080/range2386080/1
component: BBa_I13458

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type: Range
persistent identity: range2386080
display id: range2386080
version: 1
direction: direction/reverse
top level: BBa_K1333312
start: 1
end: 1114
orientation: inline

BBa_K1333002
type: SequenceAnnotation
persistent identity: annotation2386087
display id: annotation2386087
version: 1
top level: BBa_K1333312
location: https://synbiohub.org/public/igem/BBa_K1333312/annotation2386087/range2386087/1
component: BBa_K1333002

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type: Range
persistent identity: range2386087
display id: range2386087
version: 1
direction: direction/reverse
top level: BBa_K1333312
start: 1279
end: 2619
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386083
display id: annotation2386083
version: 1
top level: BBa_K1333312
location: https://synbiohub.org/public/igem/BBa_K1333312/annotation2386083/range2386083/1
component: BBa_B0034

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type: Range
persistent identity: range2386083
display id: range2386083
version: 1
direction: direction/reverse
top level: BBa_K1333312
start: 1261
end: 1272
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2386081
display id: annotation2386081
version: 1
top level: BBa_K1333312
location: https://synbiohub.org/public/igem/BBa_K1333312/annotation2386081/range2386081/1
component: BBa_I13453

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type: Range
persistent identity: range2386081
display id: range2386081
version: 1
direction: direction/reverse
top level: BBa_K1333312
start: 1123
end: 1252
orientation: inline

BBa_K1316015
type: ComponentDefinition
persistent identity: BBa K1316015
display id: BBa_K1316015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRhamnose regulating csgB and csgA curli proteins
creator: Joan Cortada Garcia
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
group u list: _1691_
m user id: 0
owner id: 19967
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is meant to express the csgB and csgA curli genes, which respectively code for the production of the curli protein that is anchored to the cell membrane (CsgB); and for the major curli protein (CsgA), which forms long fibrils with other CsgA monomers in a self-assembly process. These CsgA long fibrils bind to the anchored CsgB protein forming the amyloid-like curli structure. The expression of these two genes will, then, occur at the same time and under the same conditions (in the presence of Rhamnose in this case) This construct was designed as a negative control for BBa_K1316013 and BBa_K1316014, to see if expressing CsgA constitutively has a positive impact on the speed of curli formation
mutable notes: This construct was designed as a negative control for BBa_K1316013 and BBa_K1316014, to see if expressing CsgA constitutively has a positive impact on the speed of curli formation
mutable provenance: csgA and csgB were PCRed out of the K540000 Lyon 2011 iGEM part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: start
sequence annotation: stop
sequence annotation: csgA
sequence annotation: csgB
sequence annotation: rbs
sequence annotation: Rhamnose promoter
sequence: https://synbiohub.org/public/igem/BBa_K1316015_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2399921
display id: annotation2399921
version: 1
top level: BBa_K1316015
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1316015/annotation2399921/range2399921/1

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type: Range
persistent identity: range2399921
display id: range2399921
version: 1
direction: direction/forward
top level: BBa_K1316015
start: 657
end: 659
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2399918
display id: annotation2399918
version: 1
top level: BBa_K1316015
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1316015/annotation2399918/range2399918/1

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type: Range
persistent identity: range2399918
display id: range2399918
version: 1
direction: direction/forward
top level: BBa_K1316015
start: 614
end: 616
orientation: inline

csgA
type: SequenceAnnotation
persistent identity: annotation2399919
display id: annotation2399919
version: 1
top level: BBa_K1316015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1316015/annotation2399919/range2399919/1

https://synbiohub.org/public/igem/BBa_K1316015/annotation2399919/range2399919/1
type: Range
persistent identity: range2399919
display id: range2399919
version: 1
direction: direction/forward
top level: BBa_K1316015
start: 657
end: 1112
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2399922
display id: annotation2399922
version: 1
top level: BBa_K1316015
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1316015/annotation2399922/range2399922/1

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type: Range
persistent identity: range2399922
display id: range2399922
version: 1
direction: direction/forward
top level: BBa_K1316015
start: 1110
end: 1112
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2399917
display id: annotation2399917
version: 1
top level: BBa_K1316015
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1316015/annotation2399917/range2399917/1

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type: Range
persistent identity: range2399917
display id: range2399917
version: 1
direction: direction/forward
top level: BBa_K1316015
start: 161
end: 163
orientation: inline

csgB
type: SequenceAnnotation
persistent identity: annotation2399916
display id: annotation2399916
version: 1
top level: BBa_K1316015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1316015/annotation2399916/range2399916/1

https://synbiohub.org/public/igem/BBa_K1316015/annotation2399916/range2399916/1
type: Range
persistent identity: range2399916
display id: range2399916
version: 1
direction: direction/forward
top level: BBa_K1316015
start: 161
end: 616
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2399914
display id: annotation2399914
version: 1
top level: BBa_K1316015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1316015/annotation2399914/range2399914/1

https://synbiohub.org/public/igem/BBa_K1316015/annotation2399914/range2399914/1
type: Range
persistent identity: range2399914
display id: range2399914
version: 1
direction: direction/forward
top level: BBa_K1316015
start: 148
end: 159
orientation: inline

Rhamnose promoter
type: SequenceAnnotation
persistent identity: annotation2399913
display id: annotation2399913
version: 1
top level: BBa_K1316015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1316015/annotation2399913/range2399913/1

https://synbiohub.org/public/igem/BBa_K1316015/annotation2399913/range2399913/1
type: Range
persistent identity: range2399913
display id: range2399913
version: 1
direction: direction/forward
top level: BBa_K1316015
start: 1
end: 122
orientation: inline

BBa_K1399004
type: ComponentDefinition
persistent identity: BBa K1399004
display id: BBa_K1399004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with LVA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-06-01T07:07:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 4206
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added LVA-ssrA degradation tag. The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LVA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3]. References [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LVA in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part E1010, the tag sequence was obtained from paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: GFP (mut3b)
sequence annotation: LVA
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399004_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383916
display id: annotation2383916
version: 1
top level: BBa_K1399004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383916/range2383916/1

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type: Range
persistent identity: range2383916
display id: range2383916
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 748
end: 750
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383914
display id: annotation2383914
version: 1
top level: BBa_K1399004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383914/range2383914/1

https://synbiohub.org/public/igem/BBa_K1399004/annotation2383914/range2383914/1
type: Range
persistent identity: range2383914
display id: range2383914
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 4
end: 714
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383917
display id: annotation2383917
version: 1
top level: BBa_K1399004
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383917/range2383917/1

https://synbiohub.org/public/igem/BBa_K1399004/annotation2383917/range2383917/1
type: Range
persistent identity: range2383917
display id: range2383917
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 751
end: 753
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2383915
display id: annotation2383915
version: 1
top level: BBa_K1399004
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383915/range2383915/1

https://synbiohub.org/public/igem/BBa_K1399004/annotation2383915/range2383915/1
type: Range
persistent identity: range2383915
display id: range2383915
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 715
end: 747
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383913
display id: annotation2383913
version: 1
top level: BBa_K1399004
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399004/annotation2383913/range2383913/1

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type: Range
persistent identity: range2383913
display id: range2383913
version: 1
direction: direction/forward
top level: BBa_K1399004
start: 1
end: 3
orientation: inline

BBa_K1333101
type: ComponentDefinition
persistent identity: BBa K1333101
display id: BBa_K1333101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS DNAQ
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnaq
mutable notes: dnaq
mutable provenance: dnaq
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0034
component: BBa_K1333111
sequence annotation: BBa_K1333111
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1333101_sequence/1

BBa_B0034
type: Component
persistent identity: component2429512
display id: component2429512
version: 1
top level: BBa_K1333101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1333111
type: Component
persistent identity: component2429514
display id: component2429514
version: 1
top level: BBa_K1333101
access: public
definition: https://synbiohub.org/public/igem/BBa_K1333111/1

BBa_K1333111
type: SequenceAnnotation
persistent identity: annotation2429514
display id: annotation2429514
version: 1
top level: BBa_K1333101
location: https://synbiohub.org/public/igem/BBa_K1333101/annotation2429514/range2429514/1
component: BBa_K1333111

https://synbiohub.org/public/igem/BBa_K1333101/annotation2429514/range2429514/1
type: Range
persistent identity: range2429514
display id: range2429514
version: 1
direction: direction/reverse
top level: BBa_K1333101
start: 19
end: 750
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2429512
display id: annotation2429512
version: 1
top level: BBa_K1333101
location: https://synbiohub.org/public/igem/BBa_K1333101/annotation2429512/range2429512/1
component: BBa_B0034

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type: Range
persistent identity: range2429512
display id: range2429512
version: 1
direction: direction/reverse
top level: BBa_K1333101
start: 1
end: 12
orientation: inline

BBa_K1505002
type: ComponentDefinition
persistent identity: BBa K1505002
display id: BBa_K1505002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+mntH+mCerulean
creator: Jane Shmushkis, Amey Vrudhula
created: 2014-10-09T11:00:00Z
modified: 2016-01-13T10:36:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1885_
m user id: 4206
owner id: 22465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The RBS is from pmsp3 promoter, the strongest promoter isolated from Magnetospirillum bacteria. mntH was functionally expressed in Azospirillum sp. B510. It encodes a divalent metal cation transporter. The MntH protein and mCerulean protein are linked by a fusion protein linker. The purpose of the linkage is to improve the expression/folding of MntH and for better visualization of successful transformation. The original use is to express MntH in Magnetospirillum magneticum strain AMB-1.
mutable notes: We researched the optimum linker sequence for fusion protein to facilitate successful fusion. The mCerulean protein is expected to improve the folding of MntH in AMB-1.
mutable provenance: RBS is predicted from pmsp3 promoter from Magnetospirillum magneticum strain AMB-1 genome. MntH is from part BBa_K1505000. mCerulean is from part BBa_K133066.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: ggggs linker
sequence annotation: rbs (from pmsp3)
sequence annotation: mCerulean
sequence annotation: mntH
sequence: https://synbiohub.org/public/igem/BBa_K1505002_sequence/1

ggggs linker
type: SequenceAnnotation
persistent identity: annotation2412329
display id: annotation2412329
version: 1
top level: BBa_K1505002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412329/range2412329/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412329/range2412329/1
type: Range
persistent identity: range2412329
display id: range2412329
version: 1
direction: direction/forward
top level: BBa_K1505002
start: 1303
end: 1317
orientation: inline

rbs (from pmsp3)
type: SequenceAnnotation
persistent identity: annotation2412327
display id: annotation2412327
version: 1
top level: BBa_K1505002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412327/range2412327/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412327/range2412327/1
type: Range
persistent identity: range2412327
display id: range2412327
version: 1
direction: direction/forward
top level: BBa_K1505002
start: 19
end: 46
orientation: inline

mCerulean
type: SequenceAnnotation
persistent identity: annotation2412330
display id: annotation2412330
version: 1
top level: BBa_K1505002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412330/range2412330/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412330/range2412330/1
type: Range
persistent identity: range2412330
display id: range2412330
version: 1
direction: direction/forward
top level: BBa_K1505002
start: 1318
end: 2034
orientation: inline

mntH
type: SequenceAnnotation
persistent identity: annotation2412328
display id: annotation2412328
version: 1
top level: BBa_K1505002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505002/annotation2412328/range2412328/1

https://synbiohub.org/public/igem/BBa_K1505002/annotation2412328/range2412328/1
type: Range
persistent identity: range2412328
display id: range2412328
version: 1
direction: direction/forward
top level: BBa_K1505002
start: 67
end: 1302
orientation: inline

BBa_K1321343
type: ComponentDefinition
persistent identity: BBa K1321343
display id: BBa_K1321343
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDclos in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:16Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: adsdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321002
component: BBa_B0105
component: BBa_K1321009
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321009
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321343_sequence/1

BBa_K1321002
type: Component
persistent identity: component2415101
display id: component2415101
version: 1
top level: BBa_K1321343
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_B0105
type: Component
persistent identity: component2415100
display id: component2415100
version: 1
top level: BBa_K1321343
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321009
type: Component
persistent identity: component2415098
display id: component2415098
version: 1
top level: BBa_K1321343
access: public
definition: BBa_K1321009

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415101
display id: annotation2415101
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415101/range2415101/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415101/range2415101/1
type: Range
persistent identity: range2415101
display id: range2415101
version: 1
direction: direction/reverse
top level: BBa_K1321343
start: 183
end: 482
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415098
display id: annotation2415098
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415098/range2415098/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415098/range2415098/1
type: Range
persistent identity: range2415098
display id: range2415098
version: 1
direction: direction/reverse
top level: BBa_K1321343
start: 1
end: 176
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415100
display id: annotation2415100
version: 1
top level: BBa_K1321343
location: https://synbiohub.org/public/igem/BBa_K1321343/annotation2415100/range2415100/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321343/annotation2415100/range2415100/1
type: Range
persistent identity: range2415100
display id: range2415100
version: 1
direction: direction/reverse
top level: BBa_K1321343
start: 177
end: 182
orientation: inline

BBa_K1333301
type: ComponentDefinition
persistent identity: BBa K1333301
display id: BBa_K1333301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13453-PrfA-RFP
creator: Runwen Yao
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15873
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a combination of a pBAD promoter(BBa_I13453), RNA thermometer(BBa_K115003), and a RFP gene.
mutable notes: no
mutable provenance: The source of the part will be its components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13453
component: BBa_E1010
component: BBa_K115003
sequence annotation: BBa_E1010
sequence annotation: BBa_K115003
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1333301_sequence/1

BBa_I13453
type: Component
persistent identity: component2385904
display id: component2385904
version: 1
top level: BBa_K1333301
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_E1010
type: Component
persistent identity: component2385910
display id: component2385910
version: 1
top level: BBa_K1333301
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K115003
type: Component
persistent identity: component2385907
display id: component2385907
version: 1
top level: BBa_K1333301
access: public
definition: https://synbiohub.org/public/igem/BBa_K115003/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2385910
display id: annotation2385910
version: 1
top level: BBa_K1333301
location: https://synbiohub.org/public/igem/BBa_K1333301/annotation2385910/range2385910/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1333301/annotation2385910/range2385910/1
type: Range
persistent identity: range2385910
display id: range2385910
version: 1
direction: direction/reverse
top level: BBa_K1333301
start: 254
end: 959
orientation: inline

BBa_K115003
type: SequenceAnnotation
persistent identity: annotation2385907
display id: annotation2385907
version: 1
top level: BBa_K1333301
location: https://synbiohub.org/public/igem/BBa_K1333301/annotation2385907/range2385907/1
component: BBa_K115003

https://synbiohub.org/public/igem/BBa_K1333301/annotation2385907/range2385907/1
type: Range
persistent identity: range2385907
display id: range2385907
version: 1
direction: direction/reverse
top level: BBa_K1333301
start: 139
end: 247
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2385904
display id: annotation2385904
version: 1
top level: BBa_K1333301
location: https://synbiohub.org/public/igem/BBa_K1333301/annotation2385904/range2385904/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1333301/annotation2385904/range2385904/1
type: Range
persistent identity: range2385904
display id: range2385904
version: 1
direction: direction/reverse
top level: BBa_K1333301
start: 1
end: 130
orientation: inline

BBa_K1434000
type: ComponentDefinition
persistent identity: BBa K1434000
display id: BBa_K1434000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cAMP receptor protein (CRP) mutant
creator: Greta Jasulaityte
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:25Z
discontinued: false
dominant: false
group u list: _1812_
m user id: 0
owner id: 20624
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Escherichia coli CRP protein is a cAMP-activated global transcription factor. CRP protein binds cAMP and leads to the activation of transcription of more than one hundred promoters. This protein enhances the possibility of RNA polymerase holoenzyme binding and initiating transcription.
mutable notes: Introduced a mutation from ACTTCA to ATAATT.
mutable provenance: GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1434000_sequence/1

1NpuE(C*)
type: ComponentDefinition
persistent identity: BBa K1362103
display id: BBa_K1362103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + NpuDnaE C-intein RFC[105] non-slicing control construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_G0000
component: BBa_K1362431
component: BBa_K1362422
component: BBa_K1362399
component: BBa_K1362421
component: BBa_K1362414
component: BBa_K1362464
component: BBa_K1362418
component: BBa_K1362465
component: BBa_K1362420
component: BBa_K1362090
sequence annotation: BBa_K1362464
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362420
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362399
sequence annotation: BBa_K1362465
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362422
sequence: https://synbiohub.org/public/igem/BBa_K1362103_sequence/1

BBa_G0000
type: Component
persistent identity: component2411562
display id: component2411562
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362431
type: Component
persistent identity: component2411566
display id: component2411566
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_K1362422
type: Component
persistent identity: component2411594
display id: component2411594
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362399
type: Component
persistent identity: component2411572
display id: component2411572
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362399/1

BBa_K1362421
type: Component
persistent identity: component2411596
display id: component2411596
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362414
type: Component
persistent identity: component2411563
display id: component2411563
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362464
type: Component
persistent identity: component2411569
display id: component2411569
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362464/1

BBa_K1362418
type: Component
persistent identity: component2411567
display id: component2411567
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362465
type: Component
persistent identity: component2411597
display id: component2411597
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362420
type: Component
persistent identity: component2411573
display id: component2411573
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362420/1

BBa_K1362090
type: Component
persistent identity: component2411561
display id: component2411561
version: 1
top level: 1NpuE(C*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362464
type: SequenceAnnotation
persistent identity: annotation2411569
display id: annotation2411569
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411569/range2411569/1
component: BBa_K1362464

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411569/range2411569/1
type: Range
persistent identity: range2411569
display id: range2411569
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 64
end: 65
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2411562
display id: annotation2411562
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411562/range2411562/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411562/range2411562/1
type: Range
persistent identity: range2411562
display id: range2411562
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 30
end: 35
orientation: inline

BBa_K1362420
type: SequenceAnnotation
persistent identity: annotation2411573
display id: annotation2411573
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411573/range2411573/1
component: BBa_K1362420

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411573/range2411573/1
type: Range
persistent identity: range2411573
display id: range2411573
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 167
end: 170
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2411563
display id: annotation2411563
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411563/range2411563/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411563/range2411563/1
type: Range
persistent identity: range2411563
display id: range2411563
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 36
end: 38
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2411561
display id: annotation2411561
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411561/range2411561/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411561/range2411561/1
type: Range
persistent identity: range2411561
display id: range2411561
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 1
end: 29
orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2411566
display id: annotation2411566
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411566/range2411566/1
component: BBa_K1362431

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411566/range2411566/1
type: Range
persistent identity: range2411566
display id: range2411566
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 39
end: 59
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2411596
display id: annotation2411596
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411596/range2411596/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411596/range2411596/1
type: Range
persistent identity: range2411596
display id: range2411596
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 1254
end: 1257
orientation: inline

BBa_K1362399
type: SequenceAnnotation
persistent identity: annotation2411572
display id: annotation2411572
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411572/range2411572/1
component: BBa_K1362399

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411572/range2411572/1
type: Range
persistent identity: range2411572
display id: range2411572
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 66
end: 166
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2411597
display id: annotation2411597
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411597/range2411597/1
component: BBa_K1362465

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411597/range2411597/1
type: Range
persistent identity: range2411597
display id: range2411597
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 1258
end: 1259
orientation: inline

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2411567
display id: annotation2411567
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411567/range2411567/1
component: BBa_K1362418

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411567/range2411567/1
type: Range
persistent identity: range2411567
display id: range2411567
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 60
end: 63
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2411594
display id: annotation2411594
version: 1
top level: 1NpuE(C*)
location: https://synbiohub.org/public/igem/BBa_K1362103/annotation2411594/range2411594/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362103/annotation2411594/range2411594/1
type: Range
persistent identity: range2411594
display id: range2411594
version: 1
direction: direction/reverse
top level: 1NpuE(C*)
start: 171
end: 1253
orientation: inline

BBa_K1323009
type: ComponentDefinition
persistent identity: BBa K1323009
display id: BBa_K1323009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DsRed (RFP) Coding Sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-26T02:07:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: DsRed
sequence: https://synbiohub.org/public/igem/BBa_K1323009_sequence/1

DsRed
type: SequenceAnnotation
persistent identity: annotation2428765
display id: annotation2428765
version: 1
top level: BBa_K1323009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323009/annotation2428765/range2428765/1

https://synbiohub.org/public/igem/BBa_K1323009/annotation2428765/range2428765/1
type: Range
persistent identity: range2428765
display id: range2428765
version: 1
direction: direction/forward
top level: BBa_K1323009
start: 1
end: 678
orientation: inline

BBa_K1333303
type: ComponentDefinition
persistent identity: BBa K1333303
display id: BBa_K1333303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13453-Rose42
creator: Runwen Yao
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15873
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a combination of a pBAD promoter(BBa_I13453), RNA thermometer(BBa_K115001)
mutable provenance: The source of the part will be its components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13453
component: BBa_K115001
sequence annotation: BBa_I13453
sequence annotation: BBa_K115001
sequence: https://synbiohub.org/public/igem/BBa_K1333303_sequence/1

BBa_I13453
type: Component
persistent identity: component2385921
display id: component2385921
version: 1
top level: BBa_K1333303
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K115001
type: Component
persistent identity: component2385928
display id: component2385928
version: 1
top level: BBa_K1333303
access: public
definition: https://synbiohub.org/public/igem/BBa_K115001/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2385921
display id: annotation2385921
version: 1
top level: BBa_K1333303
location: https://synbiohub.org/public/igem/BBa_K1333303/annotation2385921/range2385921/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1333303/annotation2385921/range2385921/1
type: Range
persistent identity: range2385921
display id: range2385921
version: 1
direction: direction/reverse
top level: BBa_K1333303
start: 1
end: 130
orientation: inline

BBa_K115001
type: SequenceAnnotation
persistent identity: annotation2385928
display id: annotation2385928
version: 1
top level: BBa_K1333303
location: https://synbiohub.org/public/igem/BBa_K1333303/annotation2385928/range2385928/1
component: BBa_K115001

https://synbiohub.org/public/igem/BBa_K1333303/annotation2385928/range2385928/1
type: Range
persistent identity: range2385928
display id: range2385928
version: 1
direction: direction/reverse
top level: BBa_K1333303
start: 139
end: 234
orientation: inline

BBa_K1349000
type: ComponentDefinition
persistent identity: BBa K1349000
display id: BBa_K1349000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: serA(1-->1008bp)
creator: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1724_
m user id: 0
owner id: 6328
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct encodes a truncated version of E. coli W3110 SerA ( D-3-phosphoglycerate dehydrogenase), missing the last 75 aa of the full sequence, and without stop codon. SerA is requiered for serine biosynthesis. Following the work of Peters-Wendisch et al. 2005, this mutated version of the enzyme is expected to be no-longer inhibited by Serine, and to allow the production of a high serine concentration.
mutable notes: The part was obtained by PCR from E.coli strain W3110 and SLIC assembly. The construction removed the EcoR1 restriction sites in the gene by the silent mutations of a GAA codon to a GAG codon.
mutable provenance: Amplified by PCR from E.coli W3110 genomic DNA.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1349000_sequence/1

BBa_K1349007
type: ComponentDefinition
persistent identity: BBa K1349007
display id: BBa_K1349007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cusR-lza
creator: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1724_
m user id: 0
owner id: 6328
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Response regulator of the cusCFBA-cusRS divergent operons fused with a leucine zipper.
mutable notes: xxx
mutable provenance: Amplified from pWW2021,Whitaker et al. PNAS 2012
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1349007_sequence/1

BBa_K1415003
type: ComponentDefinition
persistent identity: BBa K1415003
display id: BBa_K1415003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAN (Agrotis ipsilon)
creator: HO, TSUNG YU
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Black cutworm (Agrotis ipsilon) Spread: This Caterpillar can be found, as various species, throughout North America. Characteristics: Cutworms common in Georgia fields are black (Agrotis ipsilon (Ashmed)), granulate (Agrotis subterranea (Fabricius)) and variegated cutworms (Peridroma saucia(Hubner)). These are moths in the family Noctuidae. Full-grown cutworm larvae are 1.5 to 2 inches long. Coloration will vary among species, but all tend to be stout-bodied caterpillars with four sets of prolegs. They have the tendency to curl into a ball when disturbed. Damage: Almost any plant can be attacked in the seedling stage. Cotton and certain vegetables sometimes have stand reductions. Climbing cutworms, such as the granulate cutworm, can be serious foliage feeders on some crops such as peanuts. Control: Bacillus thuringiensis, a widely available caterpillar-killing bacterium,is a very effective control for climbing cutworms as well as for the surface feeders.
mutable notes: No
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PBAN (Agrotis ipsilon)
sequence: https://synbiohub.org/public/igem/BBa_K1415003_sequence/1

PBAN (Agrotis ipsilon)
type: SequenceAnnotation
persistent identity: annotation2392675
display id: annotation2392675
version: 1
top level: BBa_K1415003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1415003/annotation2392675/range2392675/1

https://synbiohub.org/public/igem/BBa_K1415003/annotation2392675/range2392675/1
type: Range
persistent identity: range2392675
display id: range2392675
version: 1
direction: direction/forward
top level: BBa_K1415003
start: 1
end: 109
orientation: inline

BBa_K1351027
type: ComponentDefinition
persistent identity: BBa K1351027
display id: BBa_K1351027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Signal peptide of B. subtilis minor autolysin LytE
creator: Mona Dotzler
created: 2014-10-04T11:00:00Z
modified: 2015-06-01T12:56:24Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Signal peptide (amino acids 1 to 25) of ''Bacillus subtilis'' minor autolysin LytE (probable peptidoglycan endopeptidase, Uniprot [http://www.uniprot.org/uniprot/P54421 P54421]). Targets proteins for secretion via the Sec pathway (Tjalsma ''et. al.'', 2000). This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
mutable notes: none
mutable provenance: This part was generated by amplification from BioBrick [http://parts.igem.org/Part:BBa_K1351007 BBa_K1351007] with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3. LytE_ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC ATGAAAAAGCAAATCATTACAGCTACGACAGC LyteSP_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT TGCAGATGCCGCTCCTGCAAATAAC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351027_sequence/1

BBa_K1362111
type: ComponentDefinition
persistent identity: BBa K1362111
display id: BBa_K1362111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspDnaX C-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362403
component: BBa_G0000
component: BBa_K1362431
component: BBa_K1362090
component: BBa_K1362418
component: BBa_K1362421
component: BBa_K1362422
component: BBa_K1362414
component: BBa_K1362419
component: BBa_K1362467
component: BBa_K1362465
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362465
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362403
sequence annotation: BBa_K1362467
sequence: https://synbiohub.org/public/igem/BBa_K1362111_sequence/1

BBa_K1362403
type: Component
persistent identity: component2410369
display id: component2410369
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362403/1

BBa_G0000
type: Component
persistent identity: component2410362
display id: component2410362
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362431
type: Component
persistent identity: component2410366
display id: component2410366
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_K1362090
type: Component
persistent identity: component2410361
display id: component2410361
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362418
type: Component
persistent identity: component2410367
display id: component2410367
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362421
type: Component
persistent identity: component2410393
display id: component2410393
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362422
type: Component
persistent identity: component2410391
display id: component2410391
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362414
type: Component
persistent identity: component2410363
display id: component2410363
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362419
type: Component
persistent identity: component2410370
display id: component2410370
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362467
type: Component
persistent identity: component2410368
display id: component2410368
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362467/1

BBa_K1362465
type: Component
persistent identity: component2410394
display id: component2410394
version: 1
top level: BBa_K1362111
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2410366
display id: annotation2410366
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410366/range2410366/1
component: BBa_K1362431

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type: Range
persistent identity: range2410366
display id: range2410366
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 39
end: 59
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410361
display id: annotation2410361
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410361/range2410361/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362111/annotation2410361/range2410361/1
type: Range
persistent identity: range2410361
display id: range2410361
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 1
end: 29
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410363
display id: annotation2410363
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410363/range2410363/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362111/annotation2410363/range2410363/1
type: Range
persistent identity: range2410363
display id: range2410363
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 36
end: 38
orientation: inline

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2410367
display id: annotation2410367
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410367/range2410367/1
component: BBa_K1362418

https://synbiohub.org/public/igem/BBa_K1362111/annotation2410367/range2410367/1
type: Range
persistent identity: range2410367
display id: range2410367
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 60
end: 63
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410393
display id: annotation2410393
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410393/range2410393/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362111/annotation2410393/range2410393/1
type: Range
persistent identity: range2410393
display id: range2410393
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 1167
end: 1170
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2410370
display id: annotation2410370
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410370/range2410370/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362111/annotation2410370/range2410370/1
type: Range
persistent identity: range2410370
display id: range2410370
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 80
end: 83
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410391
display id: annotation2410391
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410391/range2410391/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362111/annotation2410391/range2410391/1
type: Range
persistent identity: range2410391
display id: range2410391
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 84
end: 1166
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2410394
display id: annotation2410394
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410394/range2410394/1
component: BBa_K1362465

https://synbiohub.org/public/igem/BBa_K1362111/annotation2410394/range2410394/1
type: Range
persistent identity: range2410394
display id: range2410394
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 1171
end: 1172
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410362
display id: annotation2410362
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410362/range2410362/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362111/annotation2410362/range2410362/1
type: Range
persistent identity: range2410362
display id: range2410362
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 30
end: 35
orientation: inline

BBa_K1362403
type: SequenceAnnotation
persistent identity: annotation2410369
display id: annotation2410369
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410369/range2410369/1
component: BBa_K1362403

https://synbiohub.org/public/igem/BBa_K1362111/annotation2410369/range2410369/1
type: Range
persistent identity: range2410369
display id: range2410369
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 66
end: 79
orientation: inline

BBa_K1362467
type: SequenceAnnotation
persistent identity: annotation2410368
display id: annotation2410368
version: 1
top level: BBa_K1362111
location: https://synbiohub.org/public/igem/BBa_K1362111/annotation2410368/range2410368/1
component: BBa_K1362467

https://synbiohub.org/public/igem/BBa_K1362111/annotation2410368/range2410368/1
type: Range
persistent identity: range2410368
display id: range2410368
version: 1
direction: direction/reverse
top level: BBa_K1362111
start: 64
end: 65
orientation: inline

BBa_K1554000
type: ComponentDefinition
persistent identity: BBa K1554000
display id: BBa_K1554000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TA29 promoter
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-29T01:38:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Anther-specific promoter
mutable notes: BioBrick compatible
mutable provenance: Genomic from Nicotiana tabacco
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1554000_sequence/1

BBa_K1351017
type: ComponentDefinition
persistent identity: BBa K1351017
display id: BBa_K1351017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SdpI with RBS: Immunity against the cannibalsim toxin sdpC of B. subtilis
creator: Philipp Popp & Roman Herzog
created: 2014-09-08T11:00:00Z
modified: 2015-06-01T08:18:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 4206
owner id: 20046
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This gene codes for the immunity against a cannibalism toxin of Bacillus subtilis namely the sdpC. The sdpI gene is regulated by a separate promoter (PsdpIR) which is responsible for the operon sdpIR. Cannibalsim toxins are mainly produced by b. subtilis in mid to late stationary phase when nutrients are limited.
mutable notes: No changes were preformed regarding the sequence referring to the bio brick standard.
mutable provenance: The sequence comes from a PCR with B. subtilis gDNA template.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K1351017_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2382809
display id: annotation2382809
version: 1
top level: BBa_K1351017
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1351017/annotation2382809/range2382809/1

https://synbiohub.org/public/igem/BBa_K1351017/annotation2382809/range2382809/1
type: Range
persistent identity: range2382809
display id: range2382809
version: 1
direction: direction/forward
top level: BBa_K1351017
start: 1
end: 333
orientation: inline

BBa_K1333308
type: ComponentDefinition
persistent identity: BBa K1333308
display id: BBa_K1333308
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13453-FourU-PⅧ
creator: Runwen Yao
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15873
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a combination of a pBAD promoter(BBa_I13453), RNA thermometer(BBa_K115002), and VIII gene from M13 phage(c).
mutable provenance: The source of the part will be its components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13453
component: BBa_K1333005
component: BBa_K115002
sequence annotation: BBa_K1333005
sequence annotation: BBa_I13453
sequence annotation: BBa_K115002
sequence: https://synbiohub.org/public/igem/BBa_K1333308_sequence/1

BBa_I13453
type: Component
persistent identity: component2386241
display id: component2386241
version: 1
top level: BBa_K1333308
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K1333005
type: Component
persistent identity: component2386249
display id: component2386249
version: 1
top level: BBa_K1333308
access: public
definition: BBa_K1333005

BBa_K115002
type: Component
persistent identity: component2386246
display id: component2386246
version: 1
top level: BBa_K1333308
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_K1333005
type: SequenceAnnotation
persistent identity: annotation2386249
display id: annotation2386249
version: 1
top level: BBa_K1333308
location: https://synbiohub.org/public/igem/BBa_K1333308/annotation2386249/range2386249/1
component: BBa_K1333005

https://synbiohub.org/public/igem/BBa_K1333308/annotation2386249/range2386249/1
type: Range
persistent identity: range2386249
display id: range2386249
version: 1
direction: direction/reverse
top level: BBa_K1333308
start: 197
end: 451
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2386241
display id: annotation2386241
version: 1
top level: BBa_K1333308
location: https://synbiohub.org/public/igem/BBa_K1333308/annotation2386241/range2386241/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1333308/annotation2386241/range2386241/1
type: Range
persistent identity: range2386241
display id: range2386241
version: 1
direction: direction/reverse
top level: BBa_K1333308
start: 1
end: 130
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2386246
display id: annotation2386246
version: 1
top level: BBa_K1333308
location: https://synbiohub.org/public/igem/BBa_K1333308/annotation2386246/range2386246/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K1333308/annotation2386246/range2386246/1
type: Range
persistent identity: range2386246
display id: range2386246
version: 1
direction: direction/reverse
top level: BBa_K1333308
start: 139
end: 190
orientation: inline

BBa_K577882
type: ComponentDefinition
persistent identity: BBa K577882
display id: BBa_K577882
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pC+AraC+pBad+RFPc
creator: Alberto Purwada
created: 2011-09-10T11:00:00Z
modified: 2015-05-08T01:12:46Z
discontinued: false
dominant: false
group u list: _748_
m user id: 0
owner id: 8431
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This reporter part is a combination of a constitutive promoter, AraC gene, a pBad promoter (inducible with arabinose), and a RFP gene. The purpose of this part is to provide the user with a simple, but complete, inducible RFP genetic device. Adding arabinose with any concentration higher than 0.01 mM seems to cause a significant expression of the RFP gene. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. This part can be inserted in a cell line that does not contain any AraC in its genome, which is important if the user is not using Top 10 cells.
mutable notes: The RBS, RFP, and Terminator were taken from BBa_J61002 using SpeI and PstI restriction enzymes.
mutable provenance: The part is obtained from ligating several biobricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13458
component: BBa_B0015
component: BBa_I13453
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_I13453
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_I13458
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K577882_sequence/1

BBa_I13458
type: Component
persistent identity: component2264035
display id: component2264035
version: 1
top level: BBa_K577882
access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_B0015
type: Component
persistent identity: component2264048
display id: component2264048
version: 1
top level: BBa_K577882
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I13453
type: Component
persistent identity: component2264036
display id: component2264036
version: 1
top level: BBa_K577882
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0034
type: Component
persistent identity: component2264038
display id: component2264038
version: 1
top level: BBa_K577882
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2264041
display id: component2264041
version: 1
top level: BBa_K577882
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2264036
display id: annotation2264036
version: 1
top level: BBa_K577882
location: https://synbiohub.org/public/igem/BBa_K577882/annotation2264036/range2264036/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K577882/annotation2264036/range2264036/1
type: Range
persistent identity: range2264036
display id: range2264036
version: 1
direction: direction/reverse
top level: BBa_K577882
start: 1123
end: 1252
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2264048
display id: annotation2264048
version: 1
top level: BBa_K577882
location: https://synbiohub.org/public/igem/BBa_K577882/annotation2264048/range2264048/1
component: BBa_B0015

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type: Range
persistent identity: range2264048
display id: range2264048
version: 1
direction: direction/reverse
top level: BBa_K577882
start: 1993
end: 2121
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2264038
display id: annotation2264038
version: 1
top level: BBa_K577882
location: https://synbiohub.org/public/igem/BBa_K577882/annotation2264038/range2264038/1
component: BBa_B0034

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type: Range
persistent identity: range2264038
display id: range2264038
version: 1
direction: direction/reverse
top level: BBa_K577882
start: 1261
end: 1272
orientation: inline

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation2264035
display id: annotation2264035
version: 1
top level: BBa_K577882
location: https://synbiohub.org/public/igem/BBa_K577882/annotation2264035/range2264035/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_K577882/annotation2264035/range2264035/1
type: Range
persistent identity: range2264035
display id: range2264035
version: 1
direction: direction/reverse
top level: BBa_K577882
start: 1
end: 1114
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2264041
display id: annotation2264041
version: 1
top level: BBa_K577882
location: https://synbiohub.org/public/igem/BBa_K577882/annotation2264041/range2264041/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K577882/annotation2264041/range2264041/1
type: Range
persistent identity: range2264041
display id: range2264041
version: 1
direction: direction/reverse
top level: BBa_K577882
start: 1279
end: 1984
orientation: inline

1NpuE(N*)
type: ComponentDefinition
persistent identity: BBa K1362102
display id: BBa_K1362102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + NpuDnaE N-intein RFC[105] non-splicing control construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362417
component: BBa_K1362422
component: BBa_K1362400
component: BBa_K1362416
component: BBa_K1362421
component: BBa_K1362414
component: BBa_K1362430
component: BBa_K1362090
component: BBa_G0000
component: BBa_K1362437
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362400
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362090
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362416
sequence: https://synbiohub.org/public/igem/BBa_K1362102_sequence/1

BBa_K1362417
type: Component
persistent identity: component2410123
display id: component2410123
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362422
type: Component
persistent identity: component2410118
display id: component2410118
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362400
type: Component
persistent identity: component2410120
display id: component2410120
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362400/1

BBa_K1362416
type: Component
persistent identity: component2410119
display id: component2410119
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362416/1

BBa_K1362421
type: Component
persistent identity: component2410128
display id: component2410128
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362414
type: Component
persistent identity: component2410097
display id: component2410097
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362430
type: Component
persistent identity: component2410126
display id: component2410126
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362090
type: Component
persistent identity: component2410095
display id: component2410095
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_G0000
type: Component
persistent identity: component2410096
display id: component2410096
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362437
type: Component
persistent identity: component2410130
display id: component2410130
version: 1
top level: 1NpuE(N*)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410118
display id: annotation2410118
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410118/range2410118/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362102/annotation2410118/range2410118/1
type: Range
persistent identity: range2410118
display id: range2410118
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 39
end: 1121
orientation: inline

BBa_K1362400
type: SequenceAnnotation
persistent identity: annotation2410120
display id: annotation2410120
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410120/range2410120/1
component: BBa_K1362400

https://synbiohub.org/public/igem/BBa_K1362102/annotation2410120/range2410120/1
type: Range
persistent identity: range2410120
display id: range2410120
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1126
end: 1427
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410130
display id: annotation2410130
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410130/range2410130/1
component: BBa_K1362437

https://synbiohub.org/public/igem/BBa_K1362102/annotation2410130/range2410130/1
type: Range
persistent identity: range2410130
display id: range2410130
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1462
end: 1464
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410097
display id: annotation2410097
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410097/range2410097/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362102/annotation2410097/range2410097/1
type: Range
persistent identity: range2410097
display id: range2410097
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 36
end: 38
orientation: inline

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410123
display id: annotation2410123
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410123/range2410123/1
component: BBa_K1362417

https://synbiohub.org/public/igem/BBa_K1362102/annotation2410123/range2410123/1
type: Range
persistent identity: range2410123
display id: range2410123
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1428
end: 1433
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410128
display id: annotation2410128
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410128/range2410128/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362102/annotation2410128/range2410128/1
type: Range
persistent identity: range2410128
display id: range2410128
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1458
end: 1461
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410095
display id: annotation2410095
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410095/range2410095/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362102/annotation2410095/range2410095/1
type: Range
persistent identity: range2410095
display id: range2410095
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1
end: 29
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410096
display id: annotation2410096
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410096/range2410096/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362102/annotation2410096/range2410096/1
type: Range
persistent identity: range2410096
display id: range2410096
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 30
end: 35
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410126
display id: annotation2410126
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410126/range2410126/1
component: BBa_K1362430

https://synbiohub.org/public/igem/BBa_K1362102/annotation2410126/range2410126/1
type: Range
persistent identity: range2410126
display id: range2410126
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1434
end: 1457
orientation: inline

BBa_K1362416
type: SequenceAnnotation
persistent identity: annotation2410119
display id: annotation2410119
version: 1
top level: 1NpuE(N*)
location: https://synbiohub.org/public/igem/BBa_K1362102/annotation2410119/range2410119/1
component: BBa_K1362416

https://synbiohub.org/public/igem/BBa_K1362102/annotation2410119/range2410119/1
type: Range
persistent identity: range2410119
display id: range2410119
version: 1
direction: direction/reverse
top level: 1NpuE(N*)
start: 1122
end: 1125
orientation: inline

Ag43+FLAG
type: ComponentDefinition
persistent identity: BBa K1352001
display id: BBa_K1352001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ag43+FLAG+MCS under the control of pBAD promoter
creator: Martyna Sroka, Ana-Maria Cujba
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1728_
m user id: 0
owner id: 22069
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Antigen 43 (sometimes called Ag43 or fluffing protein) is a phase-variable outer membrane protein encoded by flu gene. It is native to E.Coli K12 strain and is usually expressed at about 50, 000 copies/cell. Ag34 precursor is 1039 amino acids long and subsequently becomes cleaved into alpha and beta chains (499 and 488 amino acids long respectively). The beta subunit forms a β-barrel pore via which alpha-subunit translocates to the cell surface, and with which it remains non-covalently joined. The surface alpha chain can be released by a brief heat treatment at approx. 60oC. Ag43 is an autotransporter protein, therefore it possesses all information necessary for translocation to the cell surface in its coding sequence. Ag43 mediates autoaggregation, via a velcro-like mechanism (Heras et. al., 2014), and plays a role in E.coli biofilm formation. Interestingly, the alpha subunit is able to express foreign peptide sequences on E.coli cell surface if inserted just in front of codon 148 (Kj??rgaard et. al., 2002). BioBrick Bba_K1352001 is a modified version of the Bba_K1352000 BioBrick. It is composed of an Ag43 coding sequence with an in-frame FLAG epitope tag flanked by BglII and HindIII multiple cloning sites inserted within the alpha-subunit in front of codon 148 of Ag43. Expression of Ag43 is under the control of the pBAD promoter; it includes a ribosome binding site as well as two transcriptional terminators. Structure of the BioBrick was designed to allow easy insertion of foreign protein sequences of choice at codon 148 with a simple restriction digest with BglII and HindIII followed by ligation. Potential uses of this BioBrick include surface display of foreign peptide sequences, E.coli aggregation tests as well as synthetic vaccines production.
mutable notes: FLAG tag epitope flanked by multiple cloning sites was designed to be in-frame with Ag43 ORF; it is optimized for E.coli codon bias.
mutable provenance: This BioBrick was PCR amplified from Bba_K1352000; in-frame FLAG tag epitope flanked by multiple cloning sites was inserted via In-Fusion (Clonetech).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_I0500
component: BBa_B0010
component: BBa_B0034
component: BBa_K1352011
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_K1352011
sequence annotation: BBa_B0012
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_K1352001_sequence/1

BBa_B0012
type: Component
persistent identity: component2392310
display id: component2392310
version: 1
top level: Ag43+FLAG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: Component
persistent identity: component2392302
display id: component2392302
version: 1
top level: Ag43+FLAG
access: public
definition: pBad/araC

BBa_B0010
type: Component
persistent identity: component2392308
display id: component2392308
version: 1
top level: Ag43+FLAG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2392304
display id: component2392304
version: 1
top level: Ag43+FLAG
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1352011
type: Component
persistent identity: component2392307
display id: component2392307
version: 1
top level: Ag43+FLAG
access: public
definition: https://synbiohub.org/public/igem/BBa_K1352011/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2392304
display id: annotation2392304
version: 1
top level: Ag43+FLAG
location: https://synbiohub.org/public/igem/BBa_K1352001/annotation2392304/range2392304/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1352001/annotation2392304/range2392304/1
type: Range
persistent identity: range2392304
display id: range2392304
version: 1
direction: direction/reverse
top level: Ag43+FLAG
start: 1219
end: 1230
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2392308
display id: annotation2392308
version: 1
top level: Ag43+FLAG
location: https://synbiohub.org/public/igem/BBa_K1352001/annotation2392308/range2392308/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1352001/annotation2392308/range2392308/1
type: Range
persistent identity: range2392308
display id: range2392308
version: 1
direction: direction/forward
top level: Ag43+FLAG
start: 4401
end: 4480
orientation: inline

BBa_K1352011
type: SequenceAnnotation
persistent identity: annotation2392307
display id: annotation2392307
version: 1
top level: Ag43+FLAG
location: https://synbiohub.org/public/igem/BBa_K1352001/annotation2392307/range2392307/1
component: BBa_K1352011

https://synbiohub.org/public/igem/BBa_K1352001/annotation2392307/range2392307/1
type: Range
persistent identity: range2392307
display id: range2392307
version: 1
direction: direction/reverse
top level: Ag43+FLAG
start: 1237
end: 4392
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2392310
display id: annotation2392310
version: 1
top level: Ag43+FLAG
location: https://synbiohub.org/public/igem/BBa_K1352001/annotation2392310/range2392310/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1352001/annotation2392310/range2392310/1
type: Range
persistent identity: range2392310
display id: range2392310
version: 1
direction: direction/forward
top level: Ag43+FLAG
start: 4489
end: 4529
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2392302
display id: annotation2392302
version: 1
top level: Ag43+FLAG
location: https://synbiohub.org/public/igem/BBa_K1352001/annotation2392302/range2392302/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1352001/annotation2392302/range2392302/1
type: Range
persistent identity: range2392302
display id: range2392302
version: 1
direction: direction/reverse
top level: Ag43+FLAG
start: 1
end: 1210
orientation: inline

BBa_K1319042
type: ComponentDefinition
persistent identity: BBa K1319042
display id: BBa_K1319042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible iLOV
creator: Ren?? Hanke
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1694_
m user id: 0
owner id: 19522
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part can be used for IPTG-induced expression of K660004 (iLOV).
mutable notes: The part was assembled by Gibson assembly starting from K731520. The open reading frame was exchanged.
mutable provenance: The part was combined from previously existing BioBricks.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K731500
component: BBa_K660004
component: BBa_B0010
component: BBa_B0032
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K660004
sequence annotation: BBa_K731500
sequence: https://synbiohub.org/public/igem/BBa_K1319042_sequence/1

BBa_K731500
type: Component
persistent identity: component2390774
display id: component2390774
version: 1
top level: BBa_K1319042
access: public
definition: https://synbiohub.org/public/igem/BBa_K731500/1

BBa_K660004
type: Component
persistent identity: component2390779
display id: component2390779
version: 1
top level: BBa_K1319042
access: public
definition: https://synbiohub.org/public/igem/BBa_K660004/1

BBa_B0010
type: Component
persistent identity: component2390780
display id: component2390780
version: 1
top level: BBa_K1319042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component2390776
display id: component2390776
version: 1
top level: BBa_K1319042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component2390782
display id: component2390782
version: 1
top level: BBa_K1319042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2390776
display id: annotation2390776
version: 1
top level: BBa_K1319042
location: https://synbiohub.org/public/igem/BBa_K1319042/annotation2390776/range2390776/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K1319042/annotation2390776/range2390776/1
type: Range
persistent identity: range2390776
display id: range2390776
version: 1
direction: direction/forward
top level: BBa_K1319042
start: 1248
end: 1260
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2390780
display id: annotation2390780
version: 1
top level: BBa_K1319042
location: https://synbiohub.org/public/igem/BBa_K1319042/annotation2390780/range2390780/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1319042/annotation2390780/range2390780/1
type: Range
persistent identity: range2390780
display id: range2390780
version: 1
direction: direction/forward
top level: BBa_K1319042
start: 1611
end: 1690
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2390782
display id: annotation2390782
version: 1
top level: BBa_K1319042
location: https://synbiohub.org/public/igem/BBa_K1319042/annotation2390782/range2390782/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1319042/annotation2390782/range2390782/1
type: Range
persistent identity: range2390782
display id: range2390782
version: 1
direction: direction/forward
top level: BBa_K1319042
start: 1699
end: 1739
orientation: inline

BBa_K660004
type: SequenceAnnotation
persistent identity: annotation2390779
display id: annotation2390779
version: 1
top level: BBa_K1319042
location: https://synbiohub.org/public/igem/BBa_K1319042/annotation2390779/range2390779/1
component: BBa_K660004

https://synbiohub.org/public/igem/BBa_K1319042/annotation2390779/range2390779/1
type: Range
persistent identity: range2390779
display id: range2390779
version: 1
direction: direction/reverse
top level: BBa_K1319042
start: 1267
end: 1602
orientation: inline

BBa_K731500
type: SequenceAnnotation
persistent identity: annotation2390774
display id: annotation2390774
version: 1
top level: BBa_K1319042
location: https://synbiohub.org/public/igem/BBa_K1319042/annotation2390774/range2390774/1
component: BBa_K731500

https://synbiohub.org/public/igem/BBa_K1319042/annotation2390774/range2390774/1
type: Range
persistent identity: range2390774
display id: range2390774
version: 1
direction: direction/reverse
top level: BBa_K1319042
start: 1
end: 1239
orientation: inline

BBa_K1493601
type: ComponentDefinition
persistent identity: BBa K1493601
display id: BBa_K1493601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kis Antitoxin (Kis-Kid Toxin-Antitoxin System)
creator: Teresa Robert Finestra
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Kid antitoxin regulated by lacI promoter
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Kis Antitoxin
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1493601_sequence/1

Kis Antitoxin
type: SequenceAnnotation
persistent identity: annotation2424396
display id: annotation2424396
version: 1
top level: BBa_K1493601
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1493601/annotation2424396/range2424396/1

https://synbiohub.org/public/igem/BBa_K1493601/annotation2424396/range2424396/1
type: Range
persistent identity: range2424396
display id: range2424396
version: 1
direction: direction/forward
top level: BBa_K1493601
start: 12
end: 267
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2424395
display id: annotation2424395
version: 1
top level: BBa_K1493601
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1493601/annotation2424395/range2424395/1

https://synbiohub.org/public/igem/BBa_K1493601/annotation2424395/range2424395/1
type: Range
persistent identity: range2424395
display id: range2424395
version: 1
direction: direction/forward
top level: BBa_K1493601
start: 1
end: 6
orientation: inline

BBa_K1321349
type: ComponentDefinition
persistent identity: BBa K1321349
display id: BBa_K1321349
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to sfGFP in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:55Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_K1321337
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321349_sequence/1

BBa_K1321003
type: Component
persistent identity: component2415149
display id: component2415149
version: 1
top level: BBa_K1321349
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321337
type: Component
persistent identity: component2415153
display id: component2415153
version: 1
top level: BBa_K1321349
access: public
definition: BBa_K1321337

BBa_B0105
type: Component
persistent identity: component2415151
display id: component2415151
version: 1
top level: BBa_K1321349
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415151
display id: annotation2415151
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415151/range2415151/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321349/annotation2415151/range2415151/1
type: Range
persistent identity: range2415151
display id: range2415151
version: 1
direction: direction/reverse
top level: BBa_K1321349
start: 328
end: 333
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415153
display id: annotation2415153
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415153/range2415153/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321349/annotation2415153/range2415153/1
type: Range
persistent identity: range2415153
display id: range2415153
version: 1
direction: direction/reverse
top level: BBa_K1321349
start: 334
end: 1044
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415149
display id: annotation2415149
version: 1
top level: BBa_K1321349
location: https://synbiohub.org/public/igem/BBa_K1321349/annotation2415149/range2415149/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321349/annotation2415149/range2415149/1
type: Range
persistent identity: range2415149
display id: range2415149
version: 1
direction: direction/reverse
top level: BBa_K1321349
start: 1
end: 327
orientation: inline

BBa_K1365169
type: ComponentDefinition
persistent identity: BBa K1365169
display id: BBa_K1365169
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: signal sequence from USP45 fused to aiiA
creator: Rick Elbert
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:10:07Z
discontinued: false
dominant: false
group u list: _1741_
m user id: 0
owner id: 20186
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ----------------
mutable notes: - one nucleotide in the signal sequence of USP45 had to be changed because it contained the illegal restriction site PstI
mutable provenance: -aiiA was isolated out of the biobrick BBa_C0060. -ssUSP45 was isolated from the synthetic gene ssUSP45DspB made by IDT.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: TAATAA
sequence annotation: ssUSP45
sequence annotation: aiiA
sequence: https://synbiohub.org/public/igem/BBa_K1365169_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2423467
display id: annotation2423467
version: 1
top level: BBa_K1365169
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1365169/annotation2423467/range2423467/1

https://synbiohub.org/public/igem/BBa_K1365169/annotation2423467/range2423467/1
type: Range
persistent identity: range2423467
display id: range2423467
version: 1
direction: direction/forward
top level: BBa_K1365169
start: 1
end: 1
orientation: inline

TAATAA
type: SequenceAnnotation
persistent identity: annotation2390762
display id: annotation2390762
version: 1
top level: BBa_K1365169
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1365169/annotation2390762/range2390762/1

https://synbiohub.org/public/igem/BBa_K1365169/annotation2390762/range2390762/1
type: Range
persistent identity: range2390762
display id: range2390762
version: 1
direction: direction/forward
top level: BBa_K1365169
start: 828
end: 833
orientation: inline

ssUSP45
type: SequenceAnnotation
persistent identity: annotation2390760
display id: annotation2390760
version: 1
top level: BBa_K1365169
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1365169/annotation2390760/range2390760/1

https://synbiohub.org/public/igem/BBa_K1365169/annotation2390760/range2390760/1
type: Range
persistent identity: range2390760
display id: range2390760
version: 1
direction: direction/forward
top level: BBa_K1365169
start: 1
end: 80
orientation: inline

aiiA
type: SequenceAnnotation
persistent identity: annotation2390761
display id: annotation2390761
version: 1
top level: BBa_K1365169
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1365169/annotation2390761/range2390761/1

https://synbiohub.org/public/igem/BBa_K1365169/annotation2390761/range2390761/1
type: Range
persistent identity: range2390761
display id: range2390761
version: 1
direction: direction/forward
top level: BBa_K1365169
start: 81
end: 827
orientation: inline

BBa_K1357008
type: ComponentDefinition
persistent identity: BBa K1357008
display id: BBa_K1357008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tsPurple Reporter (RBS + Coding + Terminator)
creator: Matthew Tucker
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
group u list: _1733_
m user id: 0
owner id: 16772
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reporter to add onto parts to see if they are functional
mutable notes: None
mutable provenance: Coding sequence comes from jellyfish?
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1033906
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K1033906
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1357008_sequence/1

BBa_K1033906
type: Component
persistent identity: component2408693
display id: component2408693
version: 1
top level: BBa_K1357008
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033906/1

BBa_B0034
type: Component
persistent identity: component2408691
display id: component2408691
version: 1
top level: BBa_K1357008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2408700
display id: component2408700
version: 1
top level: BBa_K1357008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2408700
display id: annotation2408700
version: 1
top level: BBa_K1357008
location: https://synbiohub.org/public/igem/BBa_K1357008/annotation2408700/range2408700/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1357008/annotation2408700/range2408700/1
type: Range
persistent identity: range2408700
display id: range2408700
version: 1
direction: direction/reverse
top level: BBa_K1357008
start: 717
end: 845
orientation: inline

BBa_K1033906
type: SequenceAnnotation
persistent identity: annotation2408693
display id: annotation2408693
version: 1
top level: BBa_K1357008
location: https://synbiohub.org/public/igem/BBa_K1357008/annotation2408693/range2408693/1
component: BBa_K1033906

https://synbiohub.org/public/igem/BBa_K1357008/annotation2408693/range2408693/1
type: Range
persistent identity: range2408693
display id: range2408693
version: 1
direction: direction/reverse
top level: BBa_K1357008
start: 19
end: 708
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2408691
display id: annotation2408691
version: 1
top level: BBa_K1357008
location: https://synbiohub.org/public/igem/BBa_K1357008/annotation2408691/range2408691/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1357008/annotation2408691/range2408691/1
type: Range
persistent identity: range2408691
display id: range2408691
version: 1
direction: direction/reverse
top level: BBa_K1357008
start: 1
end: 12
orientation: inline

norC
type: ComponentDefinition
persistent identity: BBa K1356004
display id: BBa_K1356004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nitric oxide reductase (norC) from Pseudomonas aeruginosa PAO1
creator: Cameron Sargent
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1732_
m user id: 0
owner id: 20075
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This gene codes for the nitrite reductase (nirS) that converts nitrite (NO2-) into nitric oxide (NO). It is cloned into the pSB1C3
mutable notes: Nothing.
mutable provenance: P. aeruginosa PAO1.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1356004_sequence/1

SspDnaB(C)
type: ComponentDefinition
persistent identity: BBa K1362141
display id: BBa_K1362141
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspDnaB C-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362409
component: BBa_K1362465
component: BBa_K1362467
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362414
component: BBa_K1362418
component: BBa_K1362419
component: BBa_K1362422
component: BBa_K1362421
component: BBa_K1362431
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362409
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362467
sequence annotation: BBa_K1362465
sequence annotation: BBa_K1362419
sequence: https://synbiohub.org/public/igem/BBa_K1362141_sequence/1

BBa_K1362409
type: Component
persistent identity: component2430322
display id: component2430322
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362409/1

BBa_K1362465
type: Component
persistent identity: component2430347
display id: component2430347
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362467
type: Component
persistent identity: component2430321
display id: component2430321
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362467/1

BBa_G0000
type: Component
persistent identity: component2430315
display id: component2430315
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2430314
display id: component2430314
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362414
type: Component
persistent identity: component2430316
display id: component2430316
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362418
type: Component
persistent identity: component2430320
display id: component2430320
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362419
type: Component
persistent identity: component2430323
display id: component2430323
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362422
type: Component
persistent identity: component2430344
display id: component2430344
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362421
type: Component
persistent identity: component2430346
display id: component2430346
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362431
type: Component
persistent identity: component2430319
display id: component2430319
version: 1
top level: SspDnaB(C)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2430315
display id: annotation2430315
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430315/range2430315/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430315/range2430315/1
type: Range
persistent identity: range2430315
display id: range2430315
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 30
end: 35
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2430344
display id: annotation2430344
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430344/range2430344/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430344/range2430344/1
type: Range
persistent identity: range2430344
display id: range2430344
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 495
end: 1577
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2430346
display id: annotation2430346
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430346/range2430346/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430346/range2430346/1
type: Range
persistent identity: range2430346
display id: range2430346
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 1578
end: 1581
orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2430319
display id: annotation2430319
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430319/range2430319/1
component: BBa_K1362431

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430319/range2430319/1
type: Range
persistent identity: range2430319
display id: range2430319
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 39
end: 59
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2430314
display id: annotation2430314
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430314/range2430314/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430314/range2430314/1
type: Range
persistent identity: range2430314
display id: range2430314
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 1
end: 29
orientation: inline

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2430320
display id: annotation2430320
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430320/range2430320/1
component: BBa_K1362418

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430320/range2430320/1
type: Range
persistent identity: range2430320
display id: range2430320
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 60
end: 63
orientation: inline

BBa_K1362409
type: SequenceAnnotation
persistent identity: annotation2430322
display id: annotation2430322
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430322/range2430322/1
component: BBa_K1362409

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430322/range2430322/1
type: Range
persistent identity: range2430322
display id: range2430322
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 66
end: 490
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2430316
display id: annotation2430316
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430316/range2430316/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430316/range2430316/1
type: Range
persistent identity: range2430316
display id: range2430316
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 36
end: 38
orientation: inline

BBa_K1362467
type: SequenceAnnotation
persistent identity: annotation2430321
display id: annotation2430321
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430321/range2430321/1
component: BBa_K1362467

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430321/range2430321/1
type: Range
persistent identity: range2430321
display id: range2430321
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 64
end: 65
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2430347
display id: annotation2430347
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430347/range2430347/1
component: BBa_K1362465

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430347/range2430347/1
type: Range
persistent identity: range2430347
display id: range2430347
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 1582
end: 1583
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2430323
display id: annotation2430323
version: 1
top level: SspDnaB(C)
location: https://synbiohub.org/public/igem/BBa_K1362141/annotation2430323/range2430323/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362141/annotation2430323/range2430323/1
type: Range
persistent identity: range2430323
display id: range2430323
version: 1
direction: direction/reverse
top level: SspDnaB(C)
start: 491
end: 494
orientation: inline

BBa_K1472610
type: ComponentDefinition
persistent identity: BBa K1472610
display id: BBa_K1472610
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Delta 15 desaturase
creator: Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu
created: 2014-09-27T11:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1851_
m user id: 0
owner id: 23007
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This sequence encoded for the delta15 desaturase. The gene is from Synechocystis sp. and optimised for the expression in E. coli. During the fatty acid conversion process, delta 15 desaturase catalyses the conversion of linolenic acid (18:2) into alpha-Linolenic acid, ALA (18:3) by adding a cis-doube bond at carbon 15.
mutable notes: The enzyme cut site must be designed according to iGEM prefix and suffix requirements.
mutable provenance: The amino acid sequence of delta 15 desaturase is derived from Synechocystis sp. but codon optimised for the expression in E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Delta 15 desaturase
sequence: https://synbiohub.org/public/igem/BBa_K1472610_sequence/1

Delta 15 desaturase
type: SequenceAnnotation
persistent identity: annotation2390390
display id: annotation2390390
version: 1
top level: BBa_K1472610
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1472610/annotation2390390/range2390390/1

https://synbiohub.org/public/igem/BBa_K1472610/annotation2390390/range2390390/1
type: Range
persistent identity: range2390390
display id: range2390390
version: 1
direction: direction/forward
top level: BBa_K1472610
start: 1
end: 1104
orientation: inline

BBa_K1510002
type: ComponentDefinition
persistent identity: BBa K1510002
display id: BBa_K1510002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PnlmC (S.mutans)
creator: Yen-An Chang
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:47Z
discontinued: false
dominant: false
group u list: _1890_
m user id: 0
owner id: 21272
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is derived from Streptococcus mutans UA159, a gram positive bacterium. The main function of this promoter is to sense a quorum sensing chemical, competence-stimulating peptide (CSP), and trigger down string gene.
mutable notes: Since there is no accurate description of actual position of nlmC promoter in previous study, to ensure the function of this promoter, we amplified the sequence from two comE binding site( used for detecting CSP) to the last nucleotide before gene nlmC. As a result, the gene sequence consists of two comE binding site, nlmC promoter and RBS.
mutable provenance: Streptococcus mutans UA159
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: comE binding site
sequence annotation: -35 extend region
sequence annotation: -10 extend region
sequence: https://synbiohub.org/public/igem/BBa_K1510002_sequence/1

comE binding site
type: SequenceAnnotation
persistent identity: annotation2424695
display id: annotation2424695
version: 1
top level: BBa_K1510002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1510002/annotation2424695/range2424695/1

https://synbiohub.org/public/igem/BBa_K1510002/annotation2424695/range2424695/1
type: Range
persistent identity: range2424695
display id: range2424695
version: 1
direction: direction/forward
top level: BBa_K1510002
start: 11
end: 21
orientation: inline

comE binding site
type: SequenceAnnotation
persistent identity: annotation2425417
display id: annotation2425417
version: 1
top level: BBa_K1510002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1510002/annotation2425417/range2425417/1

https://synbiohub.org/public/igem/BBa_K1510002/annotation2425417/range2425417/1
type: Range
persistent identity: range2425417
display id: range2425417
version: 1
direction: direction/forward
top level: BBa_K1510002
start: 32
end: 42
orientation: inline

-35 extend region
type: SequenceAnnotation
persistent identity: annotation2425446
display id: annotation2425446
version: 1
top level: BBa_K1510002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1510002/annotation2425446/range2425446/1

https://synbiohub.org/public/igem/BBa_K1510002/annotation2425446/range2425446/1
type: Range
persistent identity: range2425446
display id: range2425446
version: 1
direction: direction/forward
top level: BBa_K1510002
start: 48
end: 53
orientation: inline

-10 extend region
type: SequenceAnnotation
persistent identity: annotation2425447
display id: annotation2425447
version: 1
top level: BBa_K1510002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1510002/annotation2425447/range2425447/1

https://synbiohub.org/public/igem/BBa_K1510002/annotation2425447/range2425447/1
type: Range
persistent identity: range2425447
display id: range2425447
version: 1
direction: direction/forward
top level: BBa_K1510002
start: 69
end: 76
orientation: inline

BBa_K1415005
type: ComponentDefinition
persistent identity: BBa K1415005
display id: BBa_K1415005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAN (Spodoptera litura)
creator: HO, TSUNG YU
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Oriental Leafworm Moth (Spodoptera litura) Spread:It is found in the Indo-Australian tropics. It is also established on most Polynesian islands where it occurs in a variety of island forms. Characteristics: Adult moths measure between 15-20 mm (0.59-0.79 inches) in length and have a wingspan of 30-38 mm (1.18-1.5 inches). Forewings are gray to reddish-brown, with a complex pattern of creamy streaks and paler lines along the veins. Hind wings are grayish-white with grayish-brown margins. Males have a blue-grey band from the upper corner (apex) to the inner margin of each forewing. Larvae have bright yellow stripes along the back and the sides. Larval color varies from pale green to dark green, Damage: Oriental Leafworm Moth Spodoptera litura is a Noctuid moth which is considered as an agricultural pest. It is also known as the Cluster caterpillar, Cotton leafworm, Tobacco cutworm, and Tropical armyworm. It has a very wide host range of over 120 plant species, including: lettuce, cabbage, beetroot, peanuts, geranium, cotton, banana, fuchsias, acacia, African oil palm, amaranth, alfalfa, strawberry, sorghum, sugarcane, tomatoes, asparagus, apple, eggplant, beet, beans, broccoli, elephants ear, horsetail she oak, corn, flax, lantana, papaya, orange, mango, leek, among many others. Control: The use of Bacillus thuringiensis (BT) may effectively control this pest. Other forms of biological, horticultural, and cultural control that have been studied include: planting near derris and garlic plants, breeding resistant plants from wild plants for example groundnuts from wild groundnuts, breeding resistant plants using bacterium Bacillus thuringiensis genes, using a Baculovirus, using the nematode Steinernema carpocapsae, and using the fly Exorista japonica.
mutable notes: No
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PBAN (Spodoptera litura)
sequence: https://synbiohub.org/public/igem/BBa_K1415005_sequence/1

PBAN (Spodoptera litura)
type: SequenceAnnotation
persistent identity: annotation2392677
display id: annotation2392677
version: 1
top level: BBa_K1415005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1415005/annotation2392677/range2392677/1

https://synbiohub.org/public/igem/BBa_K1415005/annotation2392677/range2392677/1
type: Range
persistent identity: range2392677
display id: range2392677
version: 1
direction: direction/forward
top level: BBa_K1415005
start: 1
end: 109
orientation: inline

SspDnaB(N)
type: ComponentDefinition
persistent identity: BBa K1362140
display id: BBa_K1362140
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspDnaB N-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362408
component: BBa_K1362430
component: BBa_K1362090
component: BBa_K1362437
component: BBa_K1362417
component: BBa_K1362422
component: BBa_G0000
component: BBa_K1362414
component: BBa_K1362421
component: BBa_K1362415
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362408
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362417
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362430
sequence: https://synbiohub.org/public/igem/BBa_K1362140_sequence/1

BBa_K1362408
type: Component
persistent identity: component2410570
display id: component2410570
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362408/1

BBa_K1362430
type: Component
persistent identity: component2410576
display id: component2410576
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362090
type: Component
persistent identity: component2410545
display id: component2410545
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362437
type: Component
persistent identity: component2410580
display id: component2410580
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362417
type: Component
persistent identity: component2410573
display id: component2410573
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362422
type: Component
persistent identity: component2410568
display id: component2410568
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_G0000
type: Component
persistent identity: component2410546
display id: component2410546
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362414
type: Component
persistent identity: component2410547
display id: component2410547
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362421
type: Component
persistent identity: component2410578
display id: component2410578
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362415
type: Component
persistent identity: component2410569
display id: component2410569
version: 1
top level: SspDnaB(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2410569
display id: annotation2410569
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410569/range2410569/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410569/range2410569/1
type: Range
persistent identity: range2410569
display id: range2410569
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1122
end: 1125
orientation: inline

BBa_K1362408
type: SequenceAnnotation
persistent identity: annotation2410570
display id: annotation2410570
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410570/range2410570/1
component: BBa_K1362408

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410570/range2410570/1
type: Range
persistent identity: range2410570
display id: range2410570
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1126
end: 1160
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410568
display id: annotation2410568
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410568/range2410568/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410568/range2410568/1
type: Range
persistent identity: range2410568
display id: range2410568
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 39
end: 1121
orientation: inline

BBa_K1362417
type: SequenceAnnotation
persistent identity: annotation2410573
display id: annotation2410573
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410573/range2410573/1
component: BBa_K1362417

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410573/range2410573/1
type: Range
persistent identity: range2410573
display id: range2410573
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1161
end: 1166
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410546
display id: annotation2410546
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410546/range2410546/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410546/range2410546/1
type: Range
persistent identity: range2410546
display id: range2410546
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 30
end: 35
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410545
display id: annotation2410545
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410545/range2410545/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410545/range2410545/1
type: Range
persistent identity: range2410545
display id: range2410545
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1
end: 29
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410578
display id: annotation2410578
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410578/range2410578/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410578/range2410578/1
type: Range
persistent identity: range2410578
display id: range2410578
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1191
end: 1194
orientation: inline

BBa_K1362437
type: SequenceAnnotation
persistent identity: annotation2410580
display id: annotation2410580
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410580/range2410580/1
component: BBa_K1362437

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410580/range2410580/1
type: Range
persistent identity: range2410580
display id: range2410580
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1195
end: 1197
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410547
display id: annotation2410547
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410547/range2410547/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410547/range2410547/1
type: Range
persistent identity: range2410547
display id: range2410547
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 36
end: 38
orientation: inline

BBa_K1362430
type: SequenceAnnotation
persistent identity: annotation2410576
display id: annotation2410576
version: 1
top level: SspDnaB(N)
location: https://synbiohub.org/public/igem/BBa_K1362140/annotation2410576/range2410576/1
component: BBa_K1362430

https://synbiohub.org/public/igem/BBa_K1362140/annotation2410576/range2410576/1
type: Range
persistent identity: range2410576
display id: range2410576
version: 1
direction: direction/reverse
top level: SspDnaB(N)
start: 1167
end: 1190
orientation: inline

BBa_K1321090
type: ComponentDefinition
persistent identity: BBa K1321090
display id: BBa_K1321090
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 + Linker-dCBD
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phytochelatin EC(20) +CBD2. TBC
mutable notes: Codon optimised for E.coli. TBC
mutable provenance: CBD2 is EnLinker-CBH2-EnLinker-CBH1 from T.reesei. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321005
component: BBa_K1321340
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321340
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321090_sequence/1

BBa_B0105
type: Component
persistent identity: component2414671
display id: component2414671
version: 1
top level: BBa_K1321090
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: Component
persistent identity: component2414669
display id: component2414669
version: 1
top level: BBa_K1321090
access: public
definition: BBa_K1321005

BBa_K1321340
type: Component
persistent identity: component2414673
display id: component2414673
version: 1
top level: BBa_K1321090
access: public
definition: BBa_K1321340

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414669
display id: annotation2414669
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414669/range2414669/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321090/annotation2414669/range2414669/1
type: Range
persistent identity: range2414669
display id: range2414669
version: 1
direction: direction/reverse
top level: BBa_K1321090
start: 1
end: 123
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414673
display id: annotation2414673
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414673/range2414673/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321090/annotation2414673/range2414673/1
type: Range
persistent identity: range2414673
display id: range2414673
version: 1
direction: direction/reverse
top level: BBa_K1321090
start: 130
end: 495
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414671
display id: annotation2414671
version: 1
top level: BBa_K1321090
location: https://synbiohub.org/public/igem/BBa_K1321090/annotation2414671/range2414671/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321090/annotation2414671/range2414671/1
type: Range
persistent identity: range2414671
display id: range2414671
version: 1
direction: direction/reverse
top level: BBa_K1321090
start: 124
end: 129
orientation: inline

BBa_K1446002
type: ComponentDefinition
persistent identity: BBa K1446002
display id: BBa_K1446002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: superfolder GFP with N-terminal pdu-microcompartment tag
creator: Philipp Lorenz
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1824_
m user id: 0
owner id: 19947
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: We have used superfolded GFP with the N-terminal pdu-microcompartment tag in order to get fluorescence images that would show localised spots of higher GFP-density, indicating that the microcompartment tag successfully inserts the attached protein into the microcompartments
mutable notes: We have designed Primers to amplify sfGFP from a plasmid, provided by Dr. Ciaran Kelly, in order to attach the microcompartment tag upstream of the sfGFP-coding region.
mutable provenance: We received a sample of sfGFP in a holding vector, provided by Dr. Ciaran Kelly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: ribosome binding site
sequence annotation: pdu-microcompartment tag
sequence annotation: superfolder GFP coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K1446002_sequence/1

ribosome binding site
type: SequenceAnnotation
persistent identity: annotation2389094
display id: annotation2389094
version: 1
top level: BBa_K1446002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1446002/annotation2389094/range2389094/1

https://synbiohub.org/public/igem/BBa_K1446002/annotation2389094/range2389094/1
type: Range
persistent identity: range2389094
display id: range2389094
version: 1
direction: direction/forward
top level: BBa_K1446002
start: 14
end: 19
orientation: inline

pdu-microcompartment tag
type: SequenceAnnotation
persistent identity: annotation2389095
display id: annotation2389095
version: 1
top level: BBa_K1446002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1446002/annotation2389095/range2389095/1

https://synbiohub.org/public/igem/BBa_K1446002/annotation2389095/range2389095/1
type: Range
persistent identity: range2389095
display id: range2389095
version: 1
direction: direction/forward
top level: BBa_K1446002
start: 31
end: 80
orientation: inline

superfolder GFP coding sequence
type: SequenceAnnotation
persistent identity: annotation2389096
display id: annotation2389096
version: 1
top level: BBa_K1446002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1446002/annotation2389096/range2389096/1

https://synbiohub.org/public/igem/BBa_K1446002/annotation2389096/range2389096/1
type: Range
persistent identity: range2389096
display id: range2389096
version: 1
direction: direction/forward
top level: BBa_K1446002
start: 81
end: 831
orientation: inline

BBa_K1362058
type: ComponentDefinition
persistent identity: BBa K1362058
display id: BBa_K1362058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PelB leading sequence as RFC[105] N-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1362444
component: BBa_K1362423
component: BBa_K1362425
component: BBa_K1362415
component: BBa_J32015
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362425
sequence annotation: BBa_J32015
sequence annotation: BBa_K1362444
sequence annotation: BBa_K1362423
sequence: https://synbiohub.org/public/igem/BBa_K1362058_sequence/1

BBa_K1362444
type: Component
persistent identity: component2409736
display id: component2409736
version: 1
top level: BBa_K1362058
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362444/1

BBa_K1362423
type: Component
persistent identity: component2409738
display id: component2409738
version: 1
top level: BBa_K1362058
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362425
type: Component
persistent identity: component2409733
display id: component2409733
version: 1
top level: BBa_K1362058
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362415
type: Component
persistent identity: component2409737
display id: component2409737
version: 1
top level: BBa_K1362058
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_J32015
type: Component
persistent identity: component2409735
display id: component2409735
version: 1
top level: BBa_K1362058
access: public
definition: https://synbiohub.org/public/igem/BBa_J32015/1

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409737
display id: annotation2409737
version: 1
top level: BBa_K1362058
location: https://synbiohub.org/public/igem/BBa_K1362058/annotation2409737/range2409737/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362058/annotation2409737/range2409737/1
type: Range
persistent identity: range2409737
display id: range2409737
version: 1
direction: direction/reverse
top level: BBa_K1362058
start: 92
end: 95
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409733
display id: annotation2409733
version: 1
top level: BBa_K1362058
location: https://synbiohub.org/public/igem/BBa_K1362058/annotation2409733/range2409733/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362058/annotation2409733/range2409733/1
type: Range
persistent identity: range2409733
display id: range2409733
version: 1
direction: direction/reverse
top level: BBa_K1362058
start: 1
end: 6
orientation: inline

BBa_J32015
type: SequenceAnnotation
persistent identity: annotation2409735
display id: annotation2409735
version: 1
top level: BBa_K1362058
location: https://synbiohub.org/public/igem/BBa_K1362058/annotation2409735/range2409735/1
component: BBa_J32015

https://synbiohub.org/public/igem/BBa_K1362058/annotation2409735/range2409735/1
type: Range
persistent identity: range2409735
display id: range2409735
version: 1
direction: direction/reverse
top level: BBa_K1362058
start: 8
end: 73
orientation: inline

BBa_K1362444
type: SequenceAnnotation
persistent identity: annotation2409736
display id: annotation2409736
version: 1
top level: BBa_K1362058
location: https://synbiohub.org/public/igem/BBa_K1362058/annotation2409736/range2409736/1
component: BBa_K1362444

https://synbiohub.org/public/igem/BBa_K1362058/annotation2409736/range2409736/1
type: Range
persistent identity: range2409736
display id: range2409736
version: 1
direction: direction/reverse
top level: BBa_K1362058
start: 74
end: 91
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409738
display id: annotation2409738
version: 1
top level: BBa_K1362058
location: https://synbiohub.org/public/igem/BBa_K1362058/annotation2409738/range2409738/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362058/annotation2409738/range2409738/1
type: Range
persistent identity: range2409738
display id: range2409738
version: 1
direction: direction/reverse
top level: BBa_K1362058
start: 96
end: 102
orientation: inline

BBa_K1330004
type: ComponentDefinition
persistent identity: BBa K1330004
display id: BBa_K1330004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23112
creator: Kristian Jensen
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
group u list: _1705_
m user id: 0
owner id: 20126
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1330004_sequence/1

Ag43+FLAG-
type: ComponentDefinition
persistent identity: BBa K1352002
display id: BBa_K1352002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ag43+FLAG+MCS(-)beta-hairpins under pBAD promoter
creator: Martyna Sroka, Ana-Maria Cujba
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1728_
m user id: 0
owner id: 22069
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Antigen 43 (sometimes called Ag43 or fluffing protein) is a phase-variable outer membrane protein encoded by flu gene. It is native to E.Coli K12 strain and is usually expressed at about 50, 000 copies/cell. Ag34 precursor is 1039 amino acids long and subsequently becomes cleaved into alpha and beta chains (499 and 488 amino acids long respectively). The beta subunit forms a β-barrel pore via which alpha-subunit translocates to the cell surface, and with which it remains non-covalently joined. The surface alpha chain can be released by a brief heat treatment at approx. 60oC. Ag43 is an autotransporter protein, therefore it possesses all information necessary for translocation to the cell surface in its coding sequence. Ag43 mediates autoaggregation, via a velcro-like mechanism (Heras et. al., 2014), and plays a role in E.coli biofilm formation. Interestingly, the alpha subunit is able to express foreign peptide sequences on E.coli cell surface if inserted just in front of codon 148 (Kj??rgaard et. al., 2002). The shape of α-subunit of Ag43 resembles letter L. It consists of a 'β-helix domain [which form] the stem of the letter L, followed by three rungs flanked by four β-hairpin motifs that bend the protein by about 110?? and a C-terminal (...) parallel β-helix domain [which forms] the bottom of the letter L' (Heras et. al., 2014). It has been indicated that this unique shape plays a crucial role in cell-to-cell aggregation via velcro-like mechanism, in which α-subunits form a dimer by coling around each other. This interaction is strengthened by Van der Waals interactions, hydrogen bonds and salt bridges facilitated by the L-shape (Heras et. al., 2014). Recent research demonstrates that disruption of the bend and straightening of the shape by removal of two β-hairpin sequences eliminates self-association of Ag43 proteins (Heras et. al., 2014). Removal of β-hairpins does not interfere with protein translocation to the cell surface membrane. This BioBrick is a modifi…
mutable notes: Maintaining ORF integrity
mutable provenance: PCR amplified from Bba_K1352001; two hairpin sequences (H1 268 AATVTGTNRLGAFSVVA 284; H2 341 GAAVSGTRSDGKAFSIG 357) were removed via In-Fusion (Clonetech).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1352012
component: BBa_I0500
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_K1352012
sequence: https://synbiohub.org/public/igem/BBa_K1352002_sequence/1

BBa_K1352012
type: Component
persistent identity: component2393254
display id: component2393254
version: 1
top level: Ag43+FLAG-
access: public
definition: https://synbiohub.org/public/igem/BBa_K1352012/1

BBa_I0500
type: Component
persistent identity: component2393249
display id: component2393249
version: 1
top level: Ag43+FLAG-
access: public
definition: pBad/araC

BBa_B0010
type: Component
persistent identity: component2393255
display id: component2393255
version: 1
top level: Ag43+FLAG-
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2393257
display id: component2393257
version: 1
top level: Ag43+FLAG-
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component2393251
display id: component2393251
version: 1
top level: Ag43+FLAG-
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393251
display id: annotation2393251
version: 1
top level: Ag43+FLAG-
location: https://synbiohub.org/public/igem/BBa_K1352002/annotation2393251/range2393251/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1352002/annotation2393251/range2393251/1
type: Range
persistent identity: range2393251
display id: range2393251
version: 1
direction: direction/reverse
top level: Ag43+FLAG-
start: 1219
end: 1230
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2393249
display id: annotation2393249
version: 1
top level: Ag43+FLAG-
location: https://synbiohub.org/public/igem/BBa_K1352002/annotation2393249/range2393249/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1352002/annotation2393249/range2393249/1
type: Range
persistent identity: range2393249
display id: range2393249
version: 1
direction: direction/reverse
top level: Ag43+FLAG-
start: 1
end: 1210
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2393255
display id: annotation2393255
version: 1
top level: Ag43+FLAG-
location: https://synbiohub.org/public/igem/BBa_K1352002/annotation2393255/range2393255/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1352002/annotation2393255/range2393255/1
type: Range
persistent identity: range2393255
display id: range2393255
version: 1
direction: direction/forward
top level: Ag43+FLAG-
start: 4299
end: 4378
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2393257
display id: annotation2393257
version: 1
top level: Ag43+FLAG-
location: https://synbiohub.org/public/igem/BBa_K1352002/annotation2393257/range2393257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1352002/annotation2393257/range2393257/1
type: Range
persistent identity: range2393257
display id: range2393257
version: 1
direction: direction/forward
top level: Ag43+FLAG-
start: 4387
end: 4427
orientation: inline

BBa_K1352012
type: SequenceAnnotation
persistent identity: annotation2393254
display id: annotation2393254
version: 1
top level: Ag43+FLAG-
location: https://synbiohub.org/public/igem/BBa_K1352002/annotation2393254/range2393254/1
component: BBa_K1352012

https://synbiohub.org/public/igem/BBa_K1352002/annotation2393254/range2393254/1
type: Range
persistent identity: range2393254
display id: range2393254
version: 1
direction: direction/reverse
top level: Ag43+FLAG-
start: 1237
end: 4290
orientation: inline

BBa_K1415009
type: ComponentDefinition
persistent identity: BBa K1415009
display id: BBa_K1415009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAN (Aedes aegypti)
creator: HO, TSUNG YU
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yellow fever mosquito (Aedes aegypyi) Spread:The yellow fever mosquito, Aedes aegypti, is a mosquito that can spread the dengue fever, chikungunya, and yellow fever viruses, and other diseases. The mosquito can be recognized by white markings on its legs and a marking in the form of a lyre on the thorax. The mosquito originated in Africa, but is now found in tropical and subtropical regions throughout the world. Characteristics: The mosquito can be recognized by white markings on its legs and a marking in the form of a lyre on the thorax. Damage: The yellow fever mosquito, Aedes aegypti, is a mosquito that can spread the dengue fever, chikungunya, and yellow fever viruses, and other diseases. Control:(1) Empty water from containers such as flower pots, birdbaths, pet water dishes, cans, gutters, tires and buckets regularly to disrupt the mosquito breeding cycle. (2)Consider using an insect repellent, be sure to follow the label directions for applying the repellent. For help selecting a mosquito repellent, try our Insect Repellent Locator.
mutable notes: No
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PBAN (Aedes aegypti)
sequence: https://synbiohub.org/public/igem/BBa_K1415009_sequence/1

PBAN (Aedes aegypti)
type: SequenceAnnotation
persistent identity: annotation2392811
display id: annotation2392811
version: 1
top level: BBa_K1415009
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1415009/annotation2392811/range2392811/1

https://synbiohub.org/public/igem/BBa_K1415009/annotation2392811/range2392811/1
type: Range
persistent identity: range2392811
display id: range2392811
version: 1
direction: direction/forward
top level: BBa_K1415009
start: 1
end: 64
orientation: inline

BBa_K1508003
type: ComponentDefinition
persistent identity: BBa K1508003
display id: BBa_K1508003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IIA(Glc) + double terminator
creator: Fahmi Dwilaksono, Indah Nurulita, Rian Adha Ardinata, Mochammad Isro Alfajri, Arief Budi Witarto
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1888_
m user id: 0
owner id: 16581
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: E. coli IIA(Glc) gene (BBa_K1508002) followed by double terminator (BBa_B0015)
mutable notes: Construct was assembled by suffix insertion, namely BBa_B0015 fragment was inserted into BBa_K1508002 backbone.
mutable provenance: IIA(Glc) gene from Escherichia coli; double terminator from iGEM Registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1508002
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K1508002
sequence: https://synbiohub.org/public/igem/BBa_K1508003_sequence/1

BBa_K1508002
type: Component
persistent identity: component2407575
display id: component2407575
version: 1
top level: BBa_K1508003
access: public
definition: BBa_K1508002

BBa_B0015
type: Component
persistent identity: component2407582
display id: component2407582
version: 1
top level: BBa_K1508003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2407582
display id: annotation2407582
version: 1
top level: BBa_K1508003
location: https://synbiohub.org/public/igem/BBa_K1508003/annotation2407582/range2407582/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1508003/annotation2407582/range2407582/1
type: Range
persistent identity: range2407582
display id: range2407582
version: 1
direction: direction/reverse
top level: BBa_K1508003
start: 522
end: 650
orientation: inline

BBa_K1508002
type: SequenceAnnotation
persistent identity: annotation2407575
display id: annotation2407575
version: 1
top level: BBa_K1508003
location: https://synbiohub.org/public/igem/BBa_K1508003/annotation2407575/range2407575/1
component: BBa_K1508002

https://synbiohub.org/public/igem/BBa_K1508003/annotation2407575/range2407575/1
type: Range
persistent identity: range2407575
display id: range2407575
version: 1
direction: direction/reverse
top level: BBa_K1508003
start: 1
end: 513
orientation: inline

ModA
type: ComponentDefinition
persistent identity: BBa K1405002
display id: BBa_K1405002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ModA
creator: Xuan Wang,Yuelong Hou
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1783_
m user id: 0
owner id: 20328
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ModA is a subunit of ModABC(ATP-binding cassette) trabsporter protein which can bind molybdenum. Molybdenum is an essential component of a large number of enzymes, where it is present in the form of two types of cofactor: the iron???molybdenum cofactor, in nitrogenase, and the molybdopterin cofactors in all the other known molybdoenzymes. Molybdenum is a relatively abundant element in the earth???s crust, in two main chemical forms, MoS2,and MoO2-. Only Mo(VI)in the form of molybdate is readily soluble and stable in aqueous solutions, and it is thus the only relevant source of molybdenum for biological systems. We want to use ModA to bind molubdenum then carry it to the plants.
mutable notes: We change several sites of the gene to evaluate the binding efficiency.
mutable provenance: It is from E.coil.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ModA
sequence: https://synbiohub.org/public/igem/BBa_K1405002_sequence/1

ModA
type: SequenceAnnotation
persistent identity: annotation2424692
display id: annotation2424692
version: 1
top level: ModA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1405002/annotation2424692/range2424692/1

https://synbiohub.org/public/igem/BBa_K1405002/annotation2424692/range2424692/1
type: Range
persistent identity: range2424692
display id: range2424692
version: 1
direction: direction/forward
top level: ModA
start: 1
end: 774
orientation: inline

BBa_K1351007
type: ComponentDefinition
persistent identity: BBa K1351007
display id: BBa_K1351007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CWB domain of B. subtilis minor autolysin LytE
creator: Mona Dotzler
created: 2014-10-04T11:00:00Z
modified: 2015-06-01T12:54:58Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cell wall binding domain and signal peptide (amino acids 1 to 207) of ''Bacillus subtilis'' minor autolysin LytE (probable peptidoglycan endopeptidase, Uniprot [http://www.uniprot.org/uniprot/P54421 P54421]). This domain has been used for displaying beta-lactamase on the cell surface of ''B. subtilis'' (Chen ''et. al.'', 2008). Used in the [http://2014.igem.org/Team:LMU-Munich/Project BaKillus project] to display pathogen-specific peptides to mediate adhesion of ''B. subtilis'' to pathogens. This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
mutable notes: Due to the N-terminal signal peptide, this part allows only C-terminal fusions of proteins to be displayed on the cell surface. The CWB domain of LytE consists of three N‐terminal LysM cell wall‐binding motifs, ranging from amino acid 28 to 193. To minimize possible effects of a translational fusion to the CWB domain, the part includes the downstream unstructered region until amino acid 207. An additional linker can be inserted to allow more structural flexibilty.
mutable provenance: This part was generated by amplification from ''B. subtilis'' W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3. LytE_ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC ATGAAAAAGCAAATCATTACAGCTACGACAGC LytE_207_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT CGATGAAGATGAAGCAGGTTTTGAGC A SpeI site was removed by changing T to C at position 399 without altering the protein sequence by fusion PCR with the following mutagenesis primers: LytE_SpeI399mut_fwd: GTCCTGAAACTGAAAGGTTCAACcAGTTCAAGCAGCTCCAGCTCATCAAAAG LytE_SpeI399mut_rev: CTTTTGATGAGCTGGAGCTGCTTGAACTgGTTGAACCTTTCAGTTTCAGGAC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LysM
sequence annotation: signal peptide
sequence: https://synbiohub.org/public/igem/BBa_K1351007_sequence/1

LysM
type: SequenceAnnotation
persistent identity: annotation2394084
display id: annotation2394084
version: 1
top level: BBa_K1351007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1351007/annotation2394084/range2394084/1

https://synbiohub.org/public/igem/BBa_K1351007/annotation2394084/range2394084/1
type: Range
persistent identity: range2394084
display id: range2394084
version: 1
direction: direction/forward
top level: BBa_K1351007
start: 84
end: 212
orientation: inline

LysM
type: SequenceAnnotation
persistent identity: annotation2394096
display id: annotation2394096
version: 1
top level: BBa_K1351007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1351007/annotation2394096/range2394096/1

https://synbiohub.org/public/igem/BBa_K1351007/annotation2394096/range2394096/1
type: Range
persistent identity: range2394096
display id: range2394096
version: 1
direction: direction/forward
top level: BBa_K1351007
start: 453
end: 581
orientation: inline

LysM
type: SequenceAnnotation
persistent identity: annotation2394085
display id: annotation2394085
version: 1
top level: BBa_K1351007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1351007/annotation2394085/range2394085/1

https://synbiohub.org/public/igem/BBa_K1351007/annotation2394085/range2394085/1
type: Range
persistent identity: range2394085
display id: range2394085
version: 1
direction: direction/forward
top level: BBa_K1351007
start: 264
end: 392
orientation: inline

signal peptide
type: SequenceAnnotation
persistent identity: annotation2394083
display id: annotation2394083
version: 1
top level: BBa_K1351007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1351007/annotation2394083/range2394083/1

https://synbiohub.org/public/igem/BBa_K1351007/annotation2394083/range2394083/1
type: Range
persistent identity: range2394083
display id: range2394083
version: 1
direction: direction/forward
top level: BBa_K1351007
start: 1
end: 75
orientation: inline

BBa_K1362205
type: ComponentDefinition
persistent identity: BBa K1362205
display id: BBa_K1362205
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A non-circularization construct (with Smt3 and His6; without RBS)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362447
component: BBa_K1362459
component: BBa_J70594
component: BBa_K1362429
component: BBa_K1362446
component: BBa_K1362424
component: BBa_K1362453
component: BBa_J04450
component: BBa_K1362458
component: BBa_K1362456
component: BBa_K1362448
component: BBa_K1362449
sequence annotation: BBa_K1362449
sequence annotation: BBa_K1362456
sequence annotation: BBa_K1362459
sequence annotation: BBa_J70594
sequence annotation: BBa_K1362458
sequence annotation: BBa_K1362448
sequence annotation: BBa_K1362429
sequence annotation: BBa_K1362446
sequence annotation: BBa_K1362424
sequence annotation: BBa_K1362453
sequence annotation: BBa_K1362447
sequence annotation: BBa_J04450
sequence: https://synbiohub.org/public/igem/BBa_K1362205_sequence/1

BBa_K1362447
type: Component
persistent identity: component2410855
display id: component2410855
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362447/1

BBa_K1362459
type: Component
persistent identity: component2410875
display id: component2410875
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362459/1

BBa_J70594
type: Component
persistent identity: component2410877
display id: component2410877
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K1362429
type: Component
persistent identity: component2410853
display id: component2410853
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362429/1

BBa_K1362446
type: Component
persistent identity: component2410847
display id: component2410847
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362446/1

BBa_K1362424
type: Component
persistent identity: component2410874
display id: component2410874
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362424/1

BBa_K1362453
type: Component
persistent identity: component2410854
display id: component2410854
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362453/1

BBa_J04450
type: Component
persistent identity: component2410873
display id: component2410873
version: 1
top level: BBa_K1362205
access: public
definition: BBa_J04450

BBa_K1362458
type: Component
persistent identity: component2410848
display id: component2410848
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362458/1

BBa_K1362456
type: Component
persistent identity: component2410850
display id: component2410850
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362456/1

BBa_K1362448
type: Component
persistent identity: component2410851
display id: component2410851
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362448/1

BBa_K1362449
type: Component
persistent identity: component2410849
display id: component2410849
version: 1
top level: BBa_K1362205
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362449/1

BBa_K1362449
type: SequenceAnnotation
persistent identity: annotation2410849
display id: annotation2410849
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410849/range2410849/1
component: BBa_K1362449

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type: Range
persistent identity: range2410849
display id: range2410849
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 31
end: 39
orientation: inline

BBa_K1362456
type: SequenceAnnotation
persistent identity: annotation2410850
display id: annotation2410850
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410850/range2410850/1
component: BBa_K1362456

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410850/range2410850/1
type: Range
persistent identity: range2410850
display id: range2410850
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 40
end: 57
orientation: inline

BBa_K1362459
type: SequenceAnnotation
persistent identity: annotation2410875
display id: annotation2410875
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410875/range2410875/1
component: BBa_K1362459

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410875/range2410875/1
type: Range
persistent identity: range2410875
display id: range2410875
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 1470
end: 1481
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2410877
display id: annotation2410877
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410877/range2410877/1
component: BBa_J70594

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type: Range
persistent identity: range2410877
display id: range2410877
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 1482
end: 1487
orientation: inline

BBa_K1362458
type: SequenceAnnotation
persistent identity: annotation2410848
display id: annotation2410848
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410848/range2410848/1
component: BBa_K1362458

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410848/range2410848/1
type: Range
persistent identity: range2410848
display id: range2410848
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 13
end: 30
orientation: inline

BBa_K1362448
type: SequenceAnnotation
persistent identity: annotation2410851
display id: annotation2410851
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410851/range2410851/1
component: BBa_K1362448

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410851/range2410851/1
type: Range
persistent identity: range2410851
display id: range2410851
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 58
end: 72
orientation: inline

BBa_K1362429
type: SequenceAnnotation
persistent identity: annotation2410853
display id: annotation2410853
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410853/range2410853/1
component: BBa_K1362429

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410853/range2410853/1
type: Range
persistent identity: range2410853
display id: range2410853
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 73
end: 366
orientation: inline

BBa_K1362446
type: SequenceAnnotation
persistent identity: annotation2410847
display id: annotation2410847
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410847/range2410847/1
component: BBa_K1362446

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410847/range2410847/1
type: Range
persistent identity: range2410847
display id: range2410847
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 1
end: 12
orientation: inline

BBa_K1362424
type: SequenceAnnotation
persistent identity: annotation2410874
display id: annotation2410874
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410874/range2410874/1
component: BBa_K1362424

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410874/range2410874/1
type: Range
persistent identity: range2410874
display id: range2410874
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 1463
end: 1469
orientation: inline

BBa_K1362453
type: SequenceAnnotation
persistent identity: annotation2410854
display id: annotation2410854
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410854/range2410854/1
component: BBa_K1362453

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410854/range2410854/1
type: Range
persistent identity: range2410854
display id: range2410854
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 367
end: 387
orientation: inline

BBa_K1362447
type: SequenceAnnotation
persistent identity: annotation2410855
display id: annotation2410855
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410855/range2410855/1
component: BBa_K1362447

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410855/range2410855/1
type: Range
persistent identity: range2410855
display id: range2410855
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 388
end: 393
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2410873
display id: annotation2410873
version: 1
top level: BBa_K1362205
location: https://synbiohub.org/public/igem/BBa_K1362205/annotation2410873/range2410873/1
component: BBa_J04450

https://synbiohub.org/public/igem/BBa_K1362205/annotation2410873/range2410873/1
type: Range
persistent identity: range2410873
display id: range2410873
version: 1
direction: direction/reverse
top level: BBa_K1362205
start: 394
end: 1462
orientation: inline

BBa_K1467201
type: ComponentDefinition
persistent identity: BBa K1467201
display id: BBa_K1467201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible AmilCP, Blue chromoprotein
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-06-01T07:04:22Z
discontinued: false
dominant: false
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A variation of the part BBa_K592009 that has been made compatible with the GoldenGate MoClo Assembly Standard as it is free from BsaI and BpiI recognition sequences. The chromoprotein naturally exhibits strong blue color when expressed. Codon-optimised for expression in dicotyledenous plants.
mutable notes: Domesticated for GoldenGate by removal of internal BsaI and BpiI recognition sequences. Codon-optimised for expression in dicotyledenous plants.
mutable provenance: Acropora millepora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467201_sequence/1

BBa_K1493804
type: ComponentDefinition
persistent identity: BBa K1493804
display id: BBa_K1493804
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter with CIλ and Tet operator sites
creator: Max van der Ploeg
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tet operator site
sequence annotation: Tet operator site
sequence annotation: promoter
sequence annotation: CI lambda operator site
sequence annotation: -34 binding site
sequence annotation: -10 binding site
sequence: https://synbiohub.org/public/igem/BBa_K1493804_sequence/1

tet operator site
type: SequenceAnnotation
persistent identity: annotation2422526
display id: annotation2422526
version: 1
top level: BBa_K1493804
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493804/annotation2422526/range2422526/1

https://synbiohub.org/public/igem/BBa_K1493804/annotation2422526/range2422526/1
type: Range
persistent identity: range2422526
display id: range2422526
version: 1
direction: direction/forward
top level: BBa_K1493804
start: 20
end: 36
orientation: inline

Tet operator site
type: SequenceAnnotation
persistent identity: annotation2422528
display id: annotation2422528
version: 1
top level: BBa_K1493804
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493804/annotation2422528/range2422528/1

https://synbiohub.org/public/igem/BBa_K1493804/annotation2422528/range2422528/1
type: Range
persistent identity: range2422528
display id: range2422528
version: 1
direction: direction/forward
top level: BBa_K1493804
start: 69
end: 86
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2422511
display id: annotation2422511
version: 1
top level: BBa_K1493804
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1493804/annotation2422511/range2422511/1

https://synbiohub.org/public/igem/BBa_K1493804/annotation2422511/range2422511/1
type: Range
persistent identity: range2422511
display id: range2422511
version: 1
direction: direction/forward
top level: BBa_K1493804
start: 1
end: 103
orientation: inline

CI lambda operator site
type: SequenceAnnotation
persistent identity: annotation2422527
display id: annotation2422527
version: 1
top level: BBa_K1493804
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493804/annotation2422527/range2422527/1

https://synbiohub.org/public/igem/BBa_K1493804/annotation2422527/range2422527/1
type: Range
persistent identity: range2422527
display id: range2422527
version: 1
direction: direction/forward
top level: BBa_K1493804
start: 46
end: 62
orientation: inline

-34 binding site
type: SequenceAnnotation
persistent identity: annotation2422512
display id: annotation2422512
version: 1
top level: BBa_K1493804
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1493804/annotation2422512/range2422512/1

https://synbiohub.org/public/igem/BBa_K1493804/annotation2422512/range2422512/1
type: Range
persistent identity: range2422512
display id: range2422512
version: 1
direction: direction/forward
top level: BBa_K1493804
start: 37
end: 42
orientation: inline

-10 binding site
type: SequenceAnnotation
persistent identity: annotation2422525
display id: annotation2422525
version: 1
top level: BBa_K1493804
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1493804/annotation2422525/range2422525/1

https://synbiohub.org/public/igem/BBa_K1493804/annotation2422525/range2422525/1
type: Range
persistent identity: range2422525
display id: range2422525
version: 1
direction: direction/forward
top level: BBa_K1493804
start: 59
end: 64
orientation: inline

BBa_K1315012
type: ComponentDefinition
persistent identity: BBa K1315012
display id: BBa_K1315012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pseudomonas AutoInducer-1 response unit (luxR promoter)
creator: Dimitrios Michailidis
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ptet + lasR + pluxR
mutable notes: All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.
mutable provenance: All the parts have come from existing iGEM distribution kits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0062
component: BBa_C0179
component: BBa_R0040
sequence annotation: BBa_C0179
sequence annotation: BBa_R0062
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1315012_sequence/1

BBa_R0062
type: Component
persistent identity: component2387464
display id: component2387464
version: 1
top level: BBa_K1315012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_C0179
type: Component
persistent identity: component2387462
display id: component2387462
version: 1
top level: BBa_K1315012
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_R0040
type: Component
persistent identity: component2387457
display id: component2387457
version: 1
top level: BBa_K1315012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2387462
display id: annotation2387462
version: 1
top level: BBa_K1315012
location: https://synbiohub.org/public/igem/BBa_K1315012/annotation2387462/range2387462/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K1315012/annotation2387462/range2387462/1
type: Range
persistent identity: range2387462
display id: range2387462
version: 1
direction: direction/reverse
top level: BBa_K1315012
start: 61
end: 783
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2387464
display id: annotation2387464
version: 1
top level: BBa_K1315012
location: https://synbiohub.org/public/igem/BBa_K1315012/annotation2387464/range2387464/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1315012/annotation2387464/range2387464/1
type: Range
persistent identity: range2387464
display id: range2387464
version: 1
direction: direction/forward
top level: BBa_K1315012
start: 792
end: 846
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2387457
display id: annotation2387457
version: 1
top level: BBa_K1315012
location: https://synbiohub.org/public/igem/BBa_K1315012/annotation2387457/range2387457/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1315012/annotation2387457/range2387457/1
type: Range
persistent identity: range2387457
display id: range2387457
version: 1
direction: direction/forward
top level: BBa_K1315012
start: 1
end: 54
orientation: inline

BBa_K1351032
type: ComponentDefinition
persistent identity: BBa K1351032
display id: BBa_K1351032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LMU Bacillus RBS collection 5
creator: Florian Riemer
created: 2014-10-05T11:00:00Z
modified: 2015-06-01T08:10:24Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20062
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: "This part was generated in a modified version of RFC25, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGATGGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined."
mutable notes: The LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1351032_sequence/1

BBa_K1491007
type: ComponentDefinition
persistent identity: BBa K1491007
display id: BBa_K1491007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-mOrange-His
creator: Danielle Peters
created: 2014-07-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mOrange with 6x His tag(BBa_K1491006) and a strong RBS (BBa_B0034). It can be used as a reporter.
mutable notes: RBS+mOrange+His
mutable provenance: RBS is BBa_B0034 and mOrange is BBa_E2050
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_K1491006
sequence annotation: BBa_K1491006
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1491007_sequence/1

BBa_B0034
type: Component
persistent identity: component2380104
display id: component2380104
version: 1
top level: BBa_K1491007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1491006
type: Component
persistent identity: component2380107
display id: component2380107
version: 1
top level: BBa_K1491007
access: public
definition: https://synbiohub.org/public/igem/BBa_K1491006/1

BBa_K1491006
type: SequenceAnnotation
persistent identity: annotation2380107
display id: annotation2380107
version: 1
top level: BBa_K1491007
location: https://synbiohub.org/public/igem/BBa_K1491007/annotation2380107/range2380107/1
component: BBa_K1491006

https://synbiohub.org/public/igem/BBa_K1491007/annotation2380107/range2380107/1
type: Range
persistent identity: range2380107
display id: range2380107
version: 1
direction: direction/reverse
top level: BBa_K1491007
start: 19
end: 765
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2380104
display id: annotation2380104
version: 1
top level: BBa_K1491007
location: https://synbiohub.org/public/igem/BBa_K1491007/annotation2380104/range2380104/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1491007/annotation2380104/range2380104/1
type: Range
persistent identity: range2380104
display id: range2380104
version: 1
direction: direction/reverse
top level: BBa_K1491007
start: 1
end: 12
orientation: inline

BBa_K1456006
type: ComponentDefinition
persistent identity: BBa K1456006
display id: BBa_K1456006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Aprotinin: Oxygen Radical Inhibitor
creator: Mustafa Semih Elitok
created: 2014-07-20T11:00:00Z
modified: 2015-05-08T01:10:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1834_
m user id: 0
owner id: 9573
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This protein prevents the production of oxygen radicals. Our cells will produce this enzyme to block the production of oxygen radicals within the cells to prevent harmful effects.
mutable notes: None
mutable provenance: We ordered this part to be synthesized. Sequence originally belongs to Bos taurus (bovine)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1456006_sequence/1

BBa_K1499000
type: ComponentDefinition
persistent identity: BBa K1499000
display id: BBa_K1499000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: wssF gene from wss operon
creator: Jotthe Kannappan
created: 2014-08-26T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
group u list: _1879_
m user id: 0
owner id: 22152
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: To do
mutable notes: NA
mutable provenance: The wss operon was extracted from P.fluorescens, an organism that produces cellulose acetate.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: wssF
sequence annotation: Start Codon
sequence annotation: Stop Codon: TGA
sequence: https://synbiohub.org/public/igem/BBa_K1499000_sequence/1

wssF
type: SequenceAnnotation
persistent identity: annotation2381638
display id: annotation2381638
version: 1
top level: BBa_K1499000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1499000/annotation2381638/range2381638/1

https://synbiohub.org/public/igem/BBa_K1499000/annotation2381638/range2381638/1
type: Range
persistent identity: range2381638
display id: range2381638
version: 1
direction: direction/forward
top level: BBa_K1499000
start: 86
end: 754
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2381639
display id: annotation2381639
version: 1
top level: BBa_K1499000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499000/annotation2381639/range2381639/1

https://synbiohub.org/public/igem/BBa_K1499000/annotation2381639/range2381639/1
type: Range
persistent identity: range2381639
display id: range2381639
version: 1
direction: direction/forward
top level: BBa_K1499000
start: 86
end: 88
orientation: inline

Stop Codon: TGA
type: SequenceAnnotation
persistent identity: annotation2381640
display id: annotation2381640
version: 1
top level: BBa_K1499000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1499000/annotation2381640/range2381640/1

https://synbiohub.org/public/igem/BBa_K1499000/annotation2381640/range2381640/1
type: Range
persistent identity: range2381640
display id: range2381640
version: 1
direction: direction/forward
top level: BBa_K1499000
start: 752
end: 754
orientation: inline

Ag43 RCF10
type: ComponentDefinition
persistent identity: BBa K1352000
display id: BBa_K1352000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ag43 with PstI sites removed under the control of pBad/araC promoter
creator: Ana-Maria Cujba, Martyna Sroka
created: 2014-09-30T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1728_
m user id: 0
owner id: 20514
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: BBa_K1352000 is the modularized version of BBa_K759001, which is compatible with iGEM Assemly Standard RCF10.
mutable notes: We found that BBa_K759001 contained 6 additional PstI sites within the coding region of Ag43. We therefore removed the additional PstI sites, to meet the requirements iGEM Assemly Standard RCF10.
mutable provenance: Rescued from BioBrick BBa_K1352000 and subjected to PCR-based site directed mutagenisis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0034
component: BBa_I0500
component: BBa_K1352010
component: BBa_B0012
sequence annotation: BBa_I0500
sequence annotation: BBa_K1352010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K1352000_sequence/1

BBa_B0010
type: Component
persistent identity: component2391590
display id: component2391590
version: 1
top level: Ag43 RCF10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2391587
display id: component2391587
version: 1
top level: Ag43 RCF10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component2391585
display id: component2391585
version: 1
top level: Ag43 RCF10
access: public
definition: pBad/araC

BBa_K1352010
type: Component
persistent identity: component2391589
display id: component2391589
version: 1
top level: Ag43 RCF10
access: public
definition: https://synbiohub.org/public/igem/BBa_K1352010/1

BBa_B0012
type: Component
persistent identity: component2391592
display id: component2391592
version: 1
top level: Ag43 RCF10
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2391585
display id: annotation2391585
version: 1
top level: Ag43 RCF10
location: https://synbiohub.org/public/igem/BBa_K1352000/annotation2391585/range2391585/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_K1352000/annotation2391585/range2391585/1
type: Range
persistent identity: range2391585
display id: range2391585
version: 1
direction: direction/reverse
top level: Ag43 RCF10
start: 1
end: 1210
orientation: inline

BBa_K1352010
type: SequenceAnnotation
persistent identity: annotation2391589
display id: annotation2391589
version: 1
top level: Ag43 RCF10
location: https://synbiohub.org/public/igem/BBa_K1352000/annotation2391589/range2391589/1
component: BBa_K1352010

https://synbiohub.org/public/igem/BBa_K1352000/annotation2391589/range2391589/1
type: Range
persistent identity: range2391589
display id: range2391589
version: 1
direction: direction/reverse
top level: Ag43 RCF10
start: 1237
end: 4356
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2391587
display id: annotation2391587
version: 1
top level: Ag43 RCF10
location: https://synbiohub.org/public/igem/BBa_K1352000/annotation2391587/range2391587/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1352000/annotation2391587/range2391587/1
type: Range
persistent identity: range2391587
display id: range2391587
version: 1
direction: direction/reverse
top level: Ag43 RCF10
start: 1219
end: 1230
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2391592
display id: annotation2391592
version: 1
top level: Ag43 RCF10
location: https://synbiohub.org/public/igem/BBa_K1352000/annotation2391592/range2391592/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K1352000/annotation2391592/range2391592/1
type: Range
persistent identity: range2391592
display id: range2391592
version: 1
direction: direction/forward
top level: Ag43 RCF10
start: 4453
end: 4493
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2391590
display id: annotation2391590
version: 1
top level: Ag43 RCF10
location: https://synbiohub.org/public/igem/BBa_K1352000/annotation2391590/range2391590/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K1352000/annotation2391590/range2391590/1
type: Range
persistent identity: range2391590
display id: range2391590
version: 1
direction: direction/forward
top level: Ag43 RCF10
start: 4365
end: 4444
orientation: inline

BBa_K1362057
type: ComponentDefinition
persistent identity: BBa K1362057
display id: BBa_K1362057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NLS as RFC[105] N-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1362415
component: BBa_K1362423
component: BBa_K1362444
component: BBa_K1362425
component: BBa_K1362440
component: BBa_J63008
sequence annotation: BBa_K1362444
sequence annotation: BBa_K1362415
sequence annotation: BBa_J63008
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362440
sequence annotation: BBa_K1362425
sequence: https://synbiohub.org/public/igem/BBa_K1362057_sequence/1

BBa_K1362415
type: Component
persistent identity: component2409729
display id: component2409729
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362423
type: Component
persistent identity: component2409730
display id: component2409730
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362444
type: Component
persistent identity: component2409728
display id: component2409728
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362444/1

BBa_K1362425
type: Component
persistent identity: component2409725
display id: component2409725
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362440
type: Component
persistent identity: component2409726
display id: component2409726
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362440/1

BBa_J63008
type: Component
persistent identity: component2409727
display id: component2409727
version: 1
top level: BBa_K1362057
access: public
definition: https://synbiohub.org/public/igem/BBa_J63008/1

BBa_K1362444
type: SequenceAnnotation
persistent identity: annotation2409728
display id: annotation2409728
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409728/range2409728/1
component: BBa_K1362444

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409728/range2409728/1
type: Range
persistent identity: range2409728
display id: range2409728
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 32
end: 49
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409729
display id: annotation2409729
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409729/range2409729/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409729/range2409729/1
type: Range
persistent identity: range2409729
display id: range2409729
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 50
end: 53
orientation: inline

BBa_J63008
type: SequenceAnnotation
persistent identity: annotation2409727
display id: annotation2409727
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409727/range2409727/1
component: BBa_J63008

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409727/range2409727/1
type: Range
persistent identity: range2409727
display id: range2409727
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 11
end: 31
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409730
display id: annotation2409730
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409730/range2409730/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409730/range2409730/1
type: Range
persistent identity: range2409730
display id: range2409730
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 54
end: 60
orientation: inline

BBa_K1362440
type: SequenceAnnotation
persistent identity: annotation2409726
display id: annotation2409726
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409726/range2409726/1
component: BBa_K1362440

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409726/range2409726/1
type: Range
persistent identity: range2409726
display id: range2409726
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 7
end: 10
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409725
display id: annotation2409725
version: 1
top level: BBa_K1362057
location: https://synbiohub.org/public/igem/BBa_K1362057/annotation2409725/range2409725/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362057/annotation2409725/range2409725/1
type: Range
persistent identity: range2409725
display id: range2409725
version: 1
direction: direction/reverse
top level: BBa_K1362057
start: 1
end: 6
orientation: inline

BBa_K1375005
type: ComponentDefinition
persistent identity: BBa K1375005
display id: BBa_K1375005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+amilCP+DT
creator: Zheng Xu
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
group u list: _1752_
m user id: 0
owner id: 18474
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a translational unit that include RBS and amilCP.
mutable notes: No
mutable provenance: B0034 K592035 B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K592025
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K592025
sequence: https://synbiohub.org/public/igem/BBa_K1375005_sequence/1

BBa_K592025
type: Component
persistent identity: component2416042
display id: component2416042
version: 1
top level: BBa_K1375005
access: public
definition: https://synbiohub.org/public/igem/BBa_K592025/1

BBa_B0015
type: Component
persistent identity: component2416049
display id: component2416049
version: 1
top level: BBa_K1375005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2416049
display id: annotation2416049
version: 1
top level: BBa_K1375005
location: https://synbiohub.org/public/igem/BBa_K1375005/annotation2416049/range2416049/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1375005/annotation2416049/range2416049/1
type: Range
persistent identity: range2416049
display id: range2416049
version: 1
direction: direction/reverse
top level: BBa_K1375005
start: 696
end: 824
orientation: inline

BBa_K592025
type: SequenceAnnotation
persistent identity: annotation2416042
display id: annotation2416042
version: 1
top level: BBa_K1375005
location: https://synbiohub.org/public/igem/BBa_K1375005/annotation2416042/range2416042/1
component: BBa_K592025

https://synbiohub.org/public/igem/BBa_K1375005/annotation2416042/range2416042/1
type: Range
persistent identity: range2416042
display id: range2416042
version: 1
direction: direction/reverse
top level: BBa_K1375005
start: 1
end: 687
orientation: inline

BBa_K1415002
type: ComponentDefinition
persistent identity: BBa K1415002
display id: BBa_K1415002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAN (Mamestra brassicae)
creator: HO, TSUNG YU
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Cotton bollworm Helicoverpa armigera （Hubner） Spread:The pink bollworm has spread to cotton-growing regions throughout the world. Characteristics: The larva is green, khaki, grey-brown or brown with dark spots. The topside is darker than the bottom side and a yellow or light brown stripe goes round the middle portion by the spots. Damage: The cotton bollworm is a moth, the larvae of which feed on a wide range of plants, including many important cultivated crops. It is a major pest in cotton and one of the most polyphagous and cosmopolitan pest species. It should not be confused with the similarly named, related species Helicoverpa zea.The cotton bollworm is a highly polyphagous species.The most important crop hosts are tomato, cotton, pigeon pea, chickpea, sorghum and cowpea. Other hosts include groundnut, okra, peas, field beans, soybeans, lucerne, Phaseolus spp., other Leguminosae, tobacco, potatoes, maize, flax, Dianthus, Rosa, Pelargonium, Chrysanthemum, a number of fruit trees, forest trees and a range of vegetable crops. Control: Cultural controls, with the exception of the use of Bt cotton and the use of mating disruption and sprays of the Entrust formulation of spinosad are acceptable to use on organically grown cotton.
mutable notes: No
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PBAN (Mamestra brassicae)
sequence: https://synbiohub.org/public/igem/BBa_K1415002_sequence/1

PBAN (Mamestra brassicae)
type: SequenceAnnotation
persistent identity: annotation2392674
display id: annotation2392674
version: 1
top level: BBa_K1415002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1415002/annotation2392674/range2392674/1

https://synbiohub.org/public/igem/BBa_K1415002/annotation2392674/range2392674/1
type: Range
persistent identity: range2392674
display id: range2392674
version: 1
direction: direction/forward
top level: BBa_K1415002
start: 1
end: 109
orientation: inline

CircSortA*
type: ComponentDefinition
persistent identity: BBa K1362003
display id: BBa_K1362003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A circularization construct (with Smt3 and His6)
creator: Silvan Schmitz
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362203
component: BBa_K1362090
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362203
sequence: https://synbiohub.org/public/igem/BBa_K1362003_sequence/1

BBa_K1362203
type: Component
persistent identity: component2409011
display id: component2409011
version: 1
top level: CircSortA*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362203/1

BBa_K1362090
type: Component
persistent identity: component2408981
display id: component2408981
version: 1
top level: CircSortA*
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2408981
display id: annotation2408981
version: 1
top level: CircSortA*
location: https://synbiohub.org/public/igem/BBa_K1362003/annotation2408981/range2408981/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362003/annotation2408981/range2408981/1
type: Range
persistent identity: range2408981
display id: range2408981
version: 1
direction: direction/reverse
top level: CircSortA*
start: 1
end: 29
orientation: inline

BBa_K1362203
type: SequenceAnnotation
persistent identity: annotation2409011
display id: annotation2409011
version: 1
top level: CircSortA*
location: https://synbiohub.org/public/igem/BBa_K1362003/annotation2409011/range2409011/1
component: BBa_K1362203

https://synbiohub.org/public/igem/BBa_K1362003/annotation2409011/range2409011/1
type: Range
persistent identity: range2409011
display id: range2409011
version: 1
direction: direction/reverse
top level: CircSortA*
start: 36
end: 1477
orientation: inline

BBa_K1334002
type: ComponentDefinition
persistent identity: BBa K1334002
display id: BBa_K1334002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HxlR+P-formaldehyde
creator: Haoyuan Sun
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1709_
m user id: 0
owner id: 22430
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: A right promoter which can sense formaldehyde while there is a left promoter which can erpress the HxlR protein theoretically.The HxlR protein can enhance the function of the P-formaldehyde.
mutable notes: The start codon of the HxlR protein is UUG.
mutable provenance: We get this part from Bacillus subtilis 168.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: HxlR coding sequence
sequence annotation: weak promoter
sequence annotation: Formaldehyde promoter
sequence annotation: HxlR protein binding site 2
sequence annotation: HxlR protein binding site 1
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1334002_sequence/1

HxlR coding sequence
type: SequenceAnnotation
persistent identity: annotation2387313
display id: annotation2387313
version: 1
top level: BBa_K1334002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387313/range2387313/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387313/range2387313/1
type: Range
persistent identity: range2387313
display id: range2387313
version: 1
direction: direction/reverse
top level: BBa_K1334002
start: 1
end: 363
orientation: inline

weak promoter
type: SequenceAnnotation
persistent identity: annotation2387310
display id: annotation2387310
version: 1
top level: BBa_K1334002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387310/range2387310/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387310/range2387310/1
type: Range
persistent identity: range2387310
display id: range2387310
version: 1
direction: direction/reverse
top level: BBa_K1334002
start: 422
end: 470
orientation: inline

Formaldehyde promoter
type: SequenceAnnotation
persistent identity: annotation2387265
display id: annotation2387265
version: 1
top level: BBa_K1334002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387265/range2387265/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387265/range2387265/1
type: Range
persistent identity: range2387265
display id: range2387265
version: 1
direction: direction/forward
top level: BBa_K1334002
start: 534
end: 570
orientation: inline

HxlR protein binding site 2
type: SequenceAnnotation
persistent identity: annotation2387311
display id: annotation2387311
version: 1
top level: BBa_K1334002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387311/range2387311/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387311/range2387311/1
type: Range
persistent identity: range2387311
display id: range2387311
version: 1
direction: direction/forward
top level: BBa_K1334002
start: 514
end: 538
orientation: inline

HxlR protein binding site 1
type: SequenceAnnotation
persistent identity: annotation2387312
display id: annotation2387312
version: 1
top level: BBa_K1334002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387312/range2387312/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387312/range2387312/1
type: Range
persistent identity: range2387312
display id: range2387312
version: 1
direction: direction/forward
top level: BBa_K1334002
start: 489
end: 513
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2387242
display id: annotation2387242
version: 1
top level: BBa_K1334002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1334002/annotation2387242/range2387242/1

https://synbiohub.org/public/igem/BBa_K1334002/annotation2387242/range2387242/1
type: Range
persistent identity: range2387242
display id: range2387242
version: 1
direction: direction/forward
top level: BBa_K1334002
start: 579
end: 586
orientation: inline

BBa_K1321340
type: ComponentDefinition
persistent identity: BBa K1321340
display id: BBa_K1321340
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Double CBD (dCBD) with N-terminal linker
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:14:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: dCBD with N-terminal linker.
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Linker
sequence annotation: dCBD
sequence annotation: cbh2 CBD
sequence annotation: cbh1 CBD
sequence: https://synbiohub.org/public/igem/BBa_K1321340_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2417850
display id: annotation2417850
version: 1
top level: BBa_K1321340
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417850/range2417850/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417850/range2417850/1
type: Range
persistent identity: range2417850
display id: range2417850
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 187
end: 258
orientation: inline

dCBD
type: SequenceAnnotation
persistent identity: annotation2417847
display id: annotation2417847
version: 1
top level: BBa_K1321340
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417847/range2417847/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417847/range2417847/1
type: Range
persistent identity: range2417847
display id: range2417847
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 1
end: 366
orientation: inline

cbh2 CBD
type: SequenceAnnotation
persistent identity: annotation2417849
display id: annotation2417849
version: 1
top level: BBa_K1321340
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417849/range2417849/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417849/range2417849/1
type: Range
persistent identity: range2417849
display id: range2417849
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 73
end: 186
orientation: inline

cbh1 CBD
type: SequenceAnnotation
persistent identity: annotation2417851
display id: annotation2417851
version: 1
top level: BBa_K1321340
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417851/range2417851/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417851/range2417851/1
type: Range
persistent identity: range2417851
display id: range2417851
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 259
end: 366
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2417848
display id: annotation2417848
version: 1
top level: BBa_K1321340
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1321340/annotation2417848/range2417848/1

https://synbiohub.org/public/igem/BBa_K1321340/annotation2417848/range2417848/1
type: Range
persistent identity: range2417848
display id: range2417848
version: 1
direction: direction/forward
top level: BBa_K1321340
start: 1
end: 72
orientation: inline

BBa_K1315007
type: ComponentDefinition
persistent identity: BBa K1315007
display id: BBa_K1315007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The BCAM0228 Response Regulator from Burkholderia
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the coding sequence of the BCAM0228 two-component regulator of the BDSF sensory BCAM0227-BCAM0228 system. This system is found in Burkholderia spp.
mutable notes: The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Burkholderia cenocepacia J2315 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BCAM0228
sequence: https://synbiohub.org/public/igem/BBa_K1315007_sequence/1

BCAM0228
type: SequenceAnnotation
persistent identity: annotation2386928
display id: annotation2386928
version: 1
top level: BBa_K1315007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315007/annotation2386928/range2386928/1

https://synbiohub.org/public/igem/BBa_K1315007/annotation2386928/range2386928/1
type: Range
persistent identity: range2386928
display id: range2386928
version: 1
direction: direction/forward
top level: BBa_K1315007
start: 1
end: 888
orientation: inline

BBa_K1349004
type: ComponentDefinition
persistent identity: BBa K1349004
display id: BBa_K1349004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mesh1
creator: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1724_
m user id: 0
owner id: 6328
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Mesh1 is involved in ppGpp hydrolysis.
mutable notes: xxx
mutable provenance: xxx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1349004_sequence/1

BBa_K1478001
type: ComponentDefinition
persistent identity: BBa K1478001
display id: BBa_K1478001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domain
creator: Fabian Fr??mling
created: 2014-09-12T11:00:00Z
modified: 2015-06-17T01:26:18Z
discontinued: false
dominant: false
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for cellulose binding domain. When fused to a protein, protein attaches to cellulose
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Domain derived from cellulose binding protein from Chlostridium cellulovorans(WP_013291799).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1478001_sequence/1

BBa_K1554001
type: ComponentDefinition
persistent identity: BBa K1554001
display id: BBa_K1554001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AtrΔ11
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:28:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Delta11 desaturase
mutable notes: BioBrick compatible
mutable provenance: It comes from Amyelois transitella.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554001_sequence/1

BBa_K1420001
type: ComponentDefinition
persistent identity: BBa K1420001
display id: BBa_K1420001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: merA, mercuric reductase from Serratia marcescens
creator: Stephen C. Heinsch
created: 2014-09-25T11:00:00Z
modified: 2015-07-22T02:51:48Z
discontinued: false
dominant: false
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Mercuric reductase catalyzes the reduction of Hg to Hg2+.
mutable notes: Mutagenesis of adenine to guanine at position 1026 to remove EcoRI site.
mutable provenance: pDU1358, donated by Anne O. Summers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merA
sequence: https://synbiohub.org/public/igem/BBa_K1420001_sequence/1

merA
type: SequenceAnnotation
persistent identity: annotation2387014
display id: annotation2387014
version: 1
top level: BBa_K1420001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420001/annotation2387014/range2387014/1

https://synbiohub.org/public/igem/BBa_K1420001/annotation2387014/range2387014/1
type: Range
persistent identity: range2387014
display id: range2387014
version: 1
direction: direction/forward
top level: BBa_K1420001
start: 1
end: 1686
orientation: inline

BBa_K1333315
type: ComponentDefinition
persistent identity: BBa K1333315
display id: BBa_K1333315
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD with araC
creator: Wei Tang
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15896
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A araBAD promoter with araC transcripted from the negative direction.
mutable notes: We generate this regulatory part to regulate our reporter gene expression in our project.
mutable provenance: The two parts are all from previous parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I13458
component: BBa_I13453
sequence annotation: BBa_I13458
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1333315_sequence/1

BBa_I13458
type: Component
persistent identity: component2386112
display id: component2386112
version: 1
top level: BBa_K1333315
access: public
definition: https://synbiohub.org/public/igem/BBa_I13458/1

BBa_I13453
type: Component
persistent identity: component2386113
display id: component2386113
version: 1
top level: BBa_K1333315
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation2386112
display id: annotation2386112
version: 1
top level: BBa_K1333315
location: https://synbiohub.org/public/igem/BBa_K1333315/annotation2386112/range2386112/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_K1333315/annotation2386112/range2386112/1
type: Range
persistent identity: range2386112
display id: range2386112
version: 1
direction: direction/reverse
top level: BBa_K1333315
start: 1
end: 1114
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2386113
display id: annotation2386113
version: 1
top level: BBa_K1333315
location: https://synbiohub.org/public/igem/BBa_K1333315/annotation2386113/range2386113/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1333315/annotation2386113/range2386113/1
type: Range
persistent identity: range2386113
display id: range2386113
version: 1
direction: direction/reverse
top level: BBa_K1333315
start: 1123
end: 1252
orientation: inline

BBa_K1321356
type: ComponentDefinition
persistent identity: BBa K1321356
display id: BBa_K1321356
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDclos driven by LacI
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: awef
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1319106
component: BBa_K1321341
component: BBa_J04500
sequence annotation: BBa_K1321341
sequence annotation: BBa_J04500
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321356_sequence/1

BBa_K1319106
type: Component
persistent identity: component2415483
display id: component2415483
version: 1
top level: BBa_K1321356
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321341
type: Component
persistent identity: component2415489
display id: component2415489
version: 1
top level: BBa_K1321356
access: public
definition: BBa_K1321341

BBa_J04500
type: Component
persistent identity: component2415481
display id: component2415481
version: 1
top level: BBa_K1321356
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1321341
type: SequenceAnnotation
persistent identity: annotation2415489
display id: annotation2415489
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
component: BBa_K1321341

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415489/range2415489/1
type: Range
persistent identity: range2415489
display id: range2415489
version: 1
direction: direction/reverse
top level: BBa_K1321356
start: 236
end: 1252
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415481
display id: annotation2415481
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415481/range2415481/1
type: Range
persistent identity: range2415481
display id: range2415481
version: 1
direction: direction/reverse
top level: BBa_K1321356
start: 1
end: 220
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415483
display id: annotation2415483
version: 1
top level: BBa_K1321356
location: https://synbiohub.org/public/igem/BBa_K1321356/annotation2415483/range2415483/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321356/annotation2415483/range2415483/1
type: Range
persistent identity: range2415483
display id: range2415483
version: 1
direction: direction/reverse
top level: BBa_K1321356
start: 221
end: 235
orientation: inline

BBa_K1445000
type: ComponentDefinition
persistent identity: BBa K1445000
display id: BBa_K1445000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13 Ori: Packaging signal for M13 phage
creator: Josephina Hendrix
created: 2014-07-27T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
group u list: _1823_
m user id: 0
owner id: 22016
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inclusion of this part on a plasmid will facilitate the uptake of the plasmid as a single-stranded phagemid into the M13 phage. Note that this part does not code for phage coat proteins so phage containing this M13 ori and your part will not multiply. Therefore, the M13K07 or similar helper phagemid is required for phage production. This part is packaged preferentially over M13K07, reducing the risk of uncontrolled phage multiplication.
mutable notes: Orientation in relation to ori may affect packaging ability. Also, 3??? end contains the promoter for the M13 gene II. This is a strong promoter and may cause unwanted expression of down stream genes. We recommend placing coding regions upstream of part.
mutable provenance: M13 ori from the Litmus 28i vector
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1445000_sequence/1

BBa_K1334000
type: ComponentDefinition
persistent identity: BBa K1334000
display id: BBa_K1334000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: A promoter activitated by formaldehyde.
creator: Haoyuan Sun
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1709_
m user id: 0
owner id: 22430
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter can sense the whether there is formaldehyde or not.It can be activitated by formaldehyde with the help of the protein hxlR.This promoter may have some leakage in the strain E.coli DH5a .
mutable notes: There may be different expression levels when you use it in different stains of E.coli.
mutable provenance: This part comes from the Bacillus subtilis genomic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: rbs
sequence annotation: HxlR protein binding site 2
sequence annotation: Formaldehyde promoter
sequence annotation: HxlR protein binding site 1
sequence annotation: A theoretically promoter(very weak)
sequence: https://synbiohub.org/public/igem/BBa_K1334000_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2386999
display id: annotation2386999
version: 1
top level: BBa_K1334000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1334000/annotation2386999/range2386999/1

https://synbiohub.org/public/igem/BBa_K1334000/annotation2386999/range2386999/1
type: Range
persistent identity: range2386999
display id: range2386999
version: 1
direction: direction/forward
top level: BBa_K1334000
start: 215
end: 222
orientation: inline

HxlR protein binding site 2
type: SequenceAnnotation
persistent identity: annotation2387004
display id: annotation2387004
version: 1
top level: BBa_K1334000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1334000/annotation2387004/range2387004/1

https://synbiohub.org/public/igem/BBa_K1334000/annotation2387004/range2387004/1
type: Range
persistent identity: range2387004
display id: range2387004
version: 1
direction: direction/forward
top level: BBa_K1334000
start: 151
end: 175
orientation: inline

Formaldehyde promoter
type: SequenceAnnotation
persistent identity: annotation2387002
display id: annotation2387002
version: 1
top level: BBa_K1334000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1334000/annotation2387002/range2387002/1

https://synbiohub.org/public/igem/BBa_K1334000/annotation2387002/range2387002/1
type: Range
persistent identity: range2387002
display id: range2387002
version: 1
direction: direction/forward
top level: BBa_K1334000
start: 171
end: 207
orientation: inline

HxlR protein binding site 1
type: SequenceAnnotation
persistent identity: annotation2387003
display id: annotation2387003
version: 1
top level: BBa_K1334000
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1334000/annotation2387003/range2387003/1

https://synbiohub.org/public/igem/BBa_K1334000/annotation2387003/range2387003/1
type: Range
persistent identity: range2387003
display id: range2387003
version: 1
direction: direction/forward
top level: BBa_K1334000
start: 126
end: 150
orientation: inline

A theoretically promoter(very weak)
type: SequenceAnnotation
persistent identity: annotation2387005
display id: annotation2387005
version: 1
top level: BBa_K1334000
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1334000/annotation2387005/range2387005/1

https://synbiohub.org/public/igem/BBa_K1334000/annotation2387005/range2387005/1
type: Range
persistent identity: range2387005
display id: range2387005
version: 1
direction: direction/reverse
top level: BBa_K1334000
start: 73
end: 107
orientation: inline

BBa_K1333105
type: ComponentDefinition
persistent identity: BBa K1333105
display id: BBa_K1333105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS recA730
creator: Yanwen Xie
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: reca730
mutable notes: reca730
mutable provenance: reca730
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1333110
component: BBa_B0034
sequence annotation: BBa_K1333110
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1333105_sequence/1

BBa_K1333110
type: Component
persistent identity: component2429530
display id: component2429530
version: 1
top level: BBa_K1333105
access: public
definition: https://synbiohub.org/public/igem/BBa_K1333110/1

BBa_B0034
type: Component
persistent identity: component2429528
display id: component2429528
version: 1
top level: BBa_K1333105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1333110
type: SequenceAnnotation
persistent identity: annotation2429530
display id: annotation2429530
version: 1
top level: BBa_K1333105
location: https://synbiohub.org/public/igem/BBa_K1333105/annotation2429530/range2429530/1
component: BBa_K1333110

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type: Range
persistent identity: range2429530
display id: range2429530
version: 1
direction: direction/reverse
top level: BBa_K1333105
start: 19
end: 1080
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2429528
display id: annotation2429528
version: 1
top level: BBa_K1333105
location: https://synbiohub.org/public/igem/BBa_K1333105/annotation2429528/range2429528/1
component: BBa_B0034

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type: Range
persistent identity: range2429528
display id: range2429528
version: 1
direction: direction/reverse
top level: BBa_K1333105
start: 1
end: 12
orientation: inline

BBa_K1316012
type: ComponentDefinition
persistent identity: BBa K1316012
display id: BBa_K1316012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 lacO + mtrCAB
creator: Joan Cortada Garcia
created: 2014-09-13T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1691_
m user id: 0
owner id: 19967
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakage MtrCAB is an electron transport complex of the bacteria Shewanella oneidensis
mutable notes: In order to eliminate the illegal restriction sites, the entire construct was PCRed in different parts and reassembled via the Golden Gate Assembly method
mutable provenance: Caroline M. Ajo-Franklin laboratory (Goldbeck et al. ACS Synth. Biol. 2013, 2, 150−159)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: start
sequence annotation: mtrB
sequence annotation: stop
sequence annotation: T7 lacO promoter
sequence annotation: mtrA
sequence annotation: RBS
sequence annotation: mtrC
sequence: https://synbiohub.org/public/igem/BBa_K1316012_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2383243
display id: annotation2383243
version: 1
top level: BBa_K1316012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383243/range2383243/1

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type: Range
persistent identity: range2383243
display id: range2383243
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 2210
end: 2212
orientation: inline

mtrB
type: SequenceAnnotation
persistent identity: annotation2383245
display id: annotation2383245
version: 1
top level: BBa_K1316012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383245/range2383245/1

https://synbiohub.org/public/igem/BBa_K1316012/annotation2383245/range2383245/1
type: Range
persistent identity: range2383245
display id: range2383245
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 3224
end: 5317
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383248
display id: annotation2383248
version: 1
top level: BBa_K1316012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383248/range2383248/1

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type: Range
persistent identity: range2383248
display id: range2383248
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 5315
end: 5317
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383241
display id: annotation2383241
version: 1
top level: BBa_K1316012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383241/range2383241/1

https://synbiohub.org/public/igem/BBa_K1316012/annotation2383241/range2383241/1
type: Range
persistent identity: range2383241
display id: range2383241
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 2139
end: 2141
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383244
display id: annotation2383244
version: 1
top level: BBa_K1316012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383244/range2383244/1

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type: Range
persistent identity: range2383244
display id: range2383244
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 3209
end: 3211
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383247
display id: annotation2383247
version: 1
top level: BBa_K1316012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383247/range2383247/1

https://synbiohub.org/public/igem/BBa_K1316012/annotation2383247/range2383247/1
type: Range
persistent identity: range2383247
display id: range2383247
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 3224
end: 3226
orientation: inline

T7 lacO promoter
type: SequenceAnnotation
persistent identity: annotation2383237
display id: annotation2383237
version: 1
top level: BBa_K1316012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383237/range2383237/1

https://synbiohub.org/public/igem/BBa_K1316012/annotation2383237/range2383237/1
type: Range
persistent identity: range2383237
display id: range2383237
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 29
end: 76
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383240
display id: annotation2383240
version: 1
top level: BBa_K1316012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383240/range2383240/1

https://synbiohub.org/public/igem/BBa_K1316012/annotation2383240/range2383240/1
type: Range
persistent identity: range2383240
display id: range2383240
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 129
end: 131
orientation: inline

mtrA
type: SequenceAnnotation
persistent identity: annotation2383242
display id: annotation2383242
version: 1
top level: BBa_K1316012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383242/range2383242/1

https://synbiohub.org/public/igem/BBa_K1316012/annotation2383242/range2383242/1
type: Range
persistent identity: range2383242
display id: range2383242
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 2210
end: 3211
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2383238
display id: annotation2383238
version: 1
top level: BBa_K1316012
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383238/range2383238/1

https://synbiohub.org/public/igem/BBa_K1316012/annotation2383238/range2383238/1
type: Range
persistent identity: range2383238
display id: range2383238
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 105
end: 109
orientation: inline

mtrC
type: SequenceAnnotation
persistent identity: annotation2383239
display id: annotation2383239
version: 1
top level: BBa_K1316012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1316012/annotation2383239/range2383239/1

https://synbiohub.org/public/igem/BBa_K1316012/annotation2383239/range2383239/1
type: Range
persistent identity: range2383239
display id: range2383239
version: 1
direction: direction/forward
top level: BBa_K1316012
start: 129
end: 2141
orientation: inline

BBa_K1323010
type: ComponentDefinition
persistent identity: BBa K1323010
display id: BBa_K1323010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: YFP Coding Sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-26T02:08:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YFP
sequence: https://synbiohub.org/public/igem/BBa_K1323010_sequence/1

YFP
type: SequenceAnnotation
persistent identity: annotation2428779
display id: annotation2428779
version: 1
top level: BBa_K1323010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323010/annotation2428779/range2428779/1

https://synbiohub.org/public/igem/BBa_K1323010/annotation2428779/range2428779/1
type: Range
persistent identity: range2428779
display id: range2428779
version: 1
direction: direction/forward
top level: BBa_K1323010
start: 1
end: 720
orientation: inline

BBa_K1321112
type: ComponentDefinition
persistent identity: BBa K1321112
display id: BBa_K1321112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to CBDcex with LacI promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhytoC+CBD7 with LacI
mutable notes: PhytoC+CBD7 with LacI
mutable provenance: PhytoC+CBD7 with LacI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_J04500
component: BBa_K1321005
component: BBa_K1319106
component: BBa_B0105
component: BBa_K1321003
sequence annotation: BBa_K1321003
sequence annotation: BBa_J04500
sequence annotation: BBa_B0105
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321112_sequence/1

BBa_J04500
type: Component
persistent identity: component2414911
display id: component2414911
version: 1
top level: BBa_K1321112
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1321005
type: Component
persistent identity: component2414915
display id: component2414915
version: 1
top level: BBa_K1321112
access: public
definition: BBa_K1321005

BBa_K1319106
type: Component
persistent identity: component2414913
display id: component2414913
version: 1
top level: BBa_K1321112
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_B0105
type: Component
persistent identity: component2414917
display id: component2414917
version: 1
top level: BBa_K1321112
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321003
type: Component
persistent identity: component2414918
display id: component2414918
version: 1
top level: BBa_K1321112
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414918
display id: annotation2414918
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414918/range2414918/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414918/range2414918/1
type: Range
persistent identity: range2414918
display id: range2414918
version: 1
direction: direction/reverse
top level: BBa_K1321112
start: 365
end: 691
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414911
display id: annotation2414911
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414911/range2414911/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414911/range2414911/1
type: Range
persistent identity: range2414911
display id: range2414911
version: 1
direction: direction/reverse
top level: BBa_K1321112
start: 1
end: 220
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414917
display id: annotation2414917
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414917/range2414917/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414917/range2414917/1
type: Range
persistent identity: range2414917
display id: range2414917
version: 1
direction: direction/reverse
top level: BBa_K1321112
start: 359
end: 364
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414913
display id: annotation2414913
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414913/range2414913/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414913/range2414913/1
type: Range
persistent identity: range2414913
display id: range2414913
version: 1
direction: direction/reverse
top level: BBa_K1321112
start: 221
end: 235
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414915
display id: annotation2414915
version: 1
top level: BBa_K1321112
location: https://synbiohub.org/public/igem/BBa_K1321112/annotation2414915/range2414915/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321112/annotation2414915/range2414915/1
type: Range
persistent identity: range2414915
display id: range2414915
version: 1
direction: direction/reverse
top level: BBa_K1321112
start: 236
end: 358
orientation: inline

BBa_K1415001
type: ComponentDefinition
persistent identity: BBa K1415001
display id: BBa_K1415001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAN (Bombyx mori)
creator: HO, TSUNG YU
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: n our project, we will biologically synthesize PBAN with our E.coli. We store the PBAN inside a trapping device (check this out at our Device page). In the device, there will be appropriate lighting and nutrient sources that will attract insects.Once an insect is attracted into our device and ingests the nutrient sources we provide, it will also inevitably come in contact with our PBAN, which is evenly mixed with the nutrient sources. As the PBAN works its magic and activates the pheromone synthesis of the attracted insect, more of this species of insect???s counterparts will be attracted and later captured.Owing to the first feature mentioned above, PBANs are species-specific, which means that it doesn't matter if other kind of insect fly into our device and eat PBANs, because the insects we don't want to catch will not be stimulated by PBANs to produce pheromone; our PBANs are only for what we want to catch, we are sure that our method won't affect other kinds of insects.
mutable notes: None
mutable provenance: Artificial synthesis from known amino acid sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PBAN (Bombyx mori)
sequence: https://synbiohub.org/public/igem/BBa_K1415001_sequence/1

PBAN (Bombyx mori)
type: SequenceAnnotation
persistent identity: annotation2391945
display id: annotation2391945
version: 1
top level: BBa_K1415001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1415001/annotation2391945/range2391945/1

https://synbiohub.org/public/igem/BBa_K1415001/annotation2391945/range2391945/1
type: Range
persistent identity: range2391945
display id: range2391945
version: 1
direction: direction/forward
top level: BBa_K1415001
start: 1
end: 109
orientation: inline

BBa_K1415004
type: ComponentDefinition
persistent identity: BBa K1415004
display id: BBa_K1415004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAN (Lymantria dispar)
creator: HO, TSUNG YU
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:18Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Gypsy Moth (Lymantria dispar) Spread: It has a range which covers Europe, Africa, and North America. Characteristics:Gypsy moth caterpillars change appearance as they grow. Young caterpillars are black or brown and about ?? inch (.6 cm) in length. As they grow, bumps develop along their backs along with coarse, black hairs. Each of the 11 sections of a developed caterpillar will have two coloured spots, the first five pairs, blue, and the last six, red. Mature caterpillars can be as long as 2 ?? inches (6.35 cm). Damage: It is classified as a pest, and its larvae consume the leaves of over 500 species of trees, shrubs and plants. The gypsy moth is one of the most destructive pests of hardwood trees in the eastern United States.he gypsy moth was considered a nuisance just ten years after their release. It included an account of all the trees being defoliated, caterpillars covering houses and sidewalks and that the caterpillars would rain down upon residents. The first outbreak occurred in 1889. An eradication program was begun in 1890. Control: Tanglefoot Pest Barrier or Sticky Tree Bands can be placed around tree trunks to help curtail the caterpillars movement into and out of the tree canopy. Apply Bacillus thuringiensis, var. kurstaki or Monterey Garden Insect Spray (Spinosad) to the leaves of trees to kill gypsy moth caterpillars.
mutable notes: No
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PBAN (Lymantria dispar)
sequence: https://synbiohub.org/public/igem/BBa_K1415004_sequence/1

PBAN (Lymantria dispar)
type: SequenceAnnotation
persistent identity: annotation2392676
display id: annotation2392676
version: 1
top level: BBa_K1415004
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1415004/annotation2392676/range2392676/1

https://synbiohub.org/public/igem/BBa_K1415004/annotation2392676/range2392676/1
type: Range
persistent identity: range2392676
display id: range2392676
version: 1
direction: direction/forward
top level: BBa_K1415004
start: 1
end: 109
orientation: inline

BBa_K1465306
type: ComponentDefinition
persistent identity: BBa K1465306
display id: BBa_K1465306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Isobutanol pathway under Ptac control
creator: iGEM-Team Bielefeld 2014
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1844_
m user id: 0
owner id: 20850
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ptac_alsS_ilvC_ilvD_kivD
mutable notes: bb
mutable provenance: bb
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K731500
component: BBa_K1465302
sequence annotation: BBa_K731500
sequence annotation: BBa_K1465302
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K731500/1

BBa_K1465302
type: Component
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display id: component2404119
version: 1
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definition: BBa_K1465302

BBa_K731500
type: SequenceAnnotation
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component: BBa_K731500

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BBa_K1465302
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component: BBa_K1465302

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SspDnaX(N)
type: ComponentDefinition
persistent identity: BBa K1362110
display id: BBa_K1362110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspDnaX N-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
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sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362422
component: BBa_K1362414
component: BBa_K1362421
component: BBa_K1362415
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362430
component: BBa_K1362402
component: BBa_K1362437
component: BBa_K1362417
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362437
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362402
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BBa_K1362422
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version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_K1362414
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top level: SspDnaX(N)
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BBa_K1362421
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version: 1
top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362415
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top level: SspDnaX(N)
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_G0000
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definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
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definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362430
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definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362402
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version: 1
top level: SspDnaX(N)
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definition: https://synbiohub.org/public/igem/BBa_K1362402/1

BBa_K1362437
type: Component
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top level: SspDnaX(N)
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BBa_K1362417
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top level: SspDnaX(N)
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definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362415
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direction: direction/reverse
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BBa_K1362421
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top level: SspDnaX(N)
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BBa_K1362414
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BBa_K1362422
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type: Range
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version: 1
direction: direction/reverse
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BBa_K1362437
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top level: SspDnaX(N)
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direction: direction/reverse
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BBa_K1362402
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top level: SspDnaX(N)
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direction: direction/reverse
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BBa_G0000
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top level: SspDnaX(N)
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direction: direction/reverse
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orientation: inline

BBa_K1362090
type: SequenceAnnotation
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component: BBa_K1362090

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type: Range
persistent identity: range2410322
display id: range2410322
version: 1
direction: direction/reverse
top level: SspDnaX(N)
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orientation: inline

BBa_K1321122
type: ComponentDefinition
persistent identity: BBa K1321122
display id: BBa_K1321122
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Smt fused to CBDcex
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321003
component: BBa_K1321011
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1321011
sequence: https://synbiohub.org/public/igem/BBa_K1321122_sequence/1

BBa_B0105
type: Component
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display id: component2414990
version: 1
top level: BBa_K1321122
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321003
type: Component
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display id: component2414991
version: 1
top level: BBa_K1321122
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321011
type: Component
persistent identity: component2414988
display id: component2414988
version: 1
top level: BBa_K1321122
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_B0105
type: SequenceAnnotation
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display id: annotation2414990
version: 1
top level: BBa_K1321122
location: https://synbiohub.org/public/igem/BBa_K1321122/annotation2414990/range2414990/1
component: BBa_B0105

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type: Range
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display id: range2414990
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direction: direction/reverse
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orientation: inline

BBa_K1321003
type: SequenceAnnotation
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display id: annotation2414991
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location: https://synbiohub.org/public/igem/BBa_K1321122/annotation2414991/range2414991/1
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type: Range
persistent identity: range2414991
display id: range2414991
version: 1
direction: direction/reverse
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BBa_K1321011
type: SequenceAnnotation
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display id: annotation2414988
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1321122/annotation2414988/range2414988/1
component: BBa_K1321011

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type: Range
persistent identity: range2414988
display id: range2414988
version: 1
direction: direction/reverse
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orientation: inline

BBa_K1434001
type: ComponentDefinition
persistent identity: BBa K1434001
display id: BBa_K1434001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glmS riboswitch
creator: Greta Jasulaityte
created: 2014-09-14T11:00:00Z
modified: 2015-05-08T01:10:25Z
discontinued: false
dominant: false
group u list: _1812_
m user id: 0
owner id: 20624
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sample status: In stock
status: status/Available
bookmark: false
mutable description: The glmS riboswitch has a self-cleavage activity through which it regulates gene expression.
mutable notes: No
mutable provenance: GenBank
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1434001_sequence/1

BBa_K1365120
type: ComponentDefinition
persistent identity: BBa K1365120
display id: BBa_K1365120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: anti Pseudomonas aeruginosa secreting system
creator: Rick Elbert
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:07Z
discontinued: false
dominant: false
group u list: _1741_
m user id: 0
owner id: 20186
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ----------------
mutable notes: ----------------------------
mutable provenance: ---------------------
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1365169
component: BBa_B0034
component: BBa_B0015
component: BBa_R0079
component: BBa_K1365111
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sequence annotation: BBa_B0015
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sequence annotation: BBa_K1365169
sequence annotation: BBa_K1365111
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BBa_K1365169
type: Component
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display id: component2401009
version: 1
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access: public
definition: BBa_K1365169

BBa_B0034
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display id: component2400999
version: 1
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definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
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display id: component2401016
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definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_R0079
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version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_K1365111
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display id: component2401003
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1365111/1

BBa_B0034
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display id: component2401005
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
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BBa_B0015
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display id: annotation2401016
version: 1
top level: BBa_K1365120
location: https://synbiohub.org/public/igem/BBa_K1365120/annotation2401016/range2401016/1
component: BBa_B0015

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type: Range
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display id: range2401016
version: 1
direction: direction/reverse
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orientation: inline

BBa_R0079
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display id: annotation2400997
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location: https://synbiohub.org/public/igem/BBa_K1365120/annotation2400997/range2400997/1
component: BBa_R0079

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BBa_K1365169
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location: https://synbiohub.org/public/igem/BBa_K1365120/annotation2401009/range2401009/1
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display id: range2401009
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BBa_B0034
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component: BBa_B0034

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type: Range
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display id: range2400999
version: 1
direction: direction/reverse
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orientation: inline

BBa_K1365111
type: SequenceAnnotation
persistent identity: annotation2401003
display id: annotation2401003
version: 1
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location: https://synbiohub.org/public/igem/BBa_K1365120/annotation2401003/range2401003/1
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type: Range
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display id: range2401003
version: 1
direction: direction/reverse
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BBa_K1321346
type: ComponentDefinition
persistent identity: BBa K1321346
display id: BBa_K1321346
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to sfGFP in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:30:31Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321337
component: BBa_K1321002
component: BBa_B0105
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321346_sequence/1

BBa_K1321337
type: Component
persistent identity: component2415125
display id: component2415125
version: 1
top level: BBa_K1321346
access: public
definition: BBa_K1321337

BBa_K1321002
type: Component
persistent identity: component2415121
display id: component2415121
version: 1
top level: BBa_K1321346
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_B0105
type: Component
persistent identity: component2415123
display id: component2415123
version: 1
top level: BBa_K1321346
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415123
display id: annotation2415123
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415123/range2415123/1
component: BBa_B0105

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type: Range
persistent identity: range2415123
display id: range2415123
version: 1
direction: direction/reverse
top level: BBa_K1321346
start: 301
end: 306
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415125
display id: annotation2415125
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415125/range2415125/1
component: BBa_K1321337

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type: Range
persistent identity: range2415125
display id: range2415125
version: 1
direction: direction/reverse
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start: 307
end: 1017
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415121
display id: annotation2415121
version: 1
top level: BBa_K1321346
location: https://synbiohub.org/public/igem/BBa_K1321346/annotation2415121/range2415121/1
component: BBa_K1321002

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type: Range
persistent identity: range2415121
display id: range2415121
version: 1
direction: direction/reverse
top level: BBa_K1321346
start: 1
end: 300
orientation: inline

BBa_K1472601
type: ComponentDefinition
persistent identity: BBa K1472601
display id: BBa_K1472601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Leaderless thioesterase
creator: Lo Hiu Fung, Lau Tsz Kit, Chu Lok Ting, Wu Chun Ting
created: 2014-09-15T11:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1851_
m user id: 0
owner id: 22977
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: We have cloned a modified thioesterase gene, ???tesA, which codes for an E. coli thioesterase protein that lacks the N-terminal 26-amino acid signal peptide sequence, and is named here as ???leaderless TesA??? or ???TesA. The ???TesA protein is expected to be expressed exclusively in the cytosol of E. coli to catalyze the conversion of fatty acyl-ACP (acyl carrier protein) or fatty acyl-CoA to free fatty acids (Figure 1). ???TesA is predicted to enhance free fatty acid production in the genetically-modified E. coli strain.
mutable notes: N/A
mutable provenance: The genome sequence of Escherichia coli K-12 substr. W3110 is available in GenBank at http://www.ncbi.nlm.nih.gov/gene/12930860.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 'tesA
sequence: https://synbiohub.org/public/igem/BBa_K1472601_sequence/1

'tesA
type: SequenceAnnotation
persistent identity: annotation2383718
display id: annotation2383718
version: 1
top level: BBa_K1472601
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1472601/annotation2383718/range2383718/1

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type: Range
persistent identity: range2383718
display id: range2383718
version: 1
direction: direction/forward
top level: BBa_K1472601
start: 1
end: 549
orientation: inline

BBa_K1470002
type: ComponentDefinition
persistent identity: BBa K1470002
display id: BBa_K1470002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Galactose-induced gene 4 DNA binding domain (Gal4DBD)
creator: Pascal Sartor
created: 2014-10-01T11:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1849_
m user id: 0
owner id: 20909
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This yeast protein is a positive transcriptional regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which are important for galactose import and conversion to glucose []. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. GAL4 is used to investigate gene expressions in several organisms (bacteria, plants, fruit flyes) []. The protein is controlled by a strong promotor. Binding to UAS-G leads to the expression of the corresponding gene. ==References== BRAND, AH., PERRIMON, N.: Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993 Jun;118(2):401-15. TRAVEN, A., JELICIC, B., SOPTA, M.: Yeast Gal4: a transcriptional paradigm revisited. EMBO Rep. May 2006; 7(5): 496???499.
mutable notes: No
mutable provenance: We received it from the AG Weber at the BIOSS Center, Freiburg, Germany.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1470002_sequence/1

BBa_K1321351
type: ComponentDefinition
persistent identity: BBa K1321351
display id: BBa_K1321351
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcenA with linker fused to NiBP in Freiburg format (RFC 25)
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:31:45Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321339
component: BBa_K1321009
component: BBa_B0105
sequence annotation: BBa_K1321009
sequence annotation: BBa_K1321339
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321351_sequence/1

BBa_K1321339
type: Component
persistent identity: component2430307
display id: component2430307
version: 1
top level: BBa_K1321351
access: public
definition: BBa_K1321339

BBa_K1321009
type: Component
persistent identity: component2430311
display id: component2430311
version: 1
top level: BBa_K1321351
access: public
definition: BBa_K1321009

BBa_B0105
type: Component
persistent identity: component2430309
display id: component2430309
version: 1
top level: BBa_K1321351
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2430311
display id: annotation2430311
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430311/range2430311/1
component: BBa_K1321009

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type: Range
persistent identity: range2430311
display id: range2430311
version: 1
direction: direction/reverse
top level: BBa_K1321351
start: 415
end: 590
orientation: inline

BBa_K1321339
type: SequenceAnnotation
persistent identity: annotation2430307
display id: annotation2430307
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430307/range2430307/1
component: BBa_K1321339

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type: Range
persistent identity: range2430307
display id: range2430307
version: 1
direction: direction/reverse
top level: BBa_K1321351
start: 1
end: 408
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2430309
display id: annotation2430309
version: 1
top level: BBa_K1321351
location: https://synbiohub.org/public/igem/BBa_K1321351/annotation2430309/range2430309/1
component: BBa_B0105

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type: Range
persistent identity: range2430309
display id: range2430309
version: 1
direction: direction/reverse
top level: BBa_K1321351
start: 409
end: 414
orientation: inline

BBa_K1399011
type: ComponentDefinition
persistent identity: BBa K1399011
display id: BBa_K1399011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LVA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LVA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. This part can be considered as improved version of BBa_J04451 as it does not contain StuI restriction site coding for additional 'RP' residues between RFP and LVA tag.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: Barcode of BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_K1399001
sequence annotation: -10
sequence annotation: end of LacI coding region (inactive)
sequence annotation: T7 TE
sequence annotation: LacI binding site
sequence annotation: stem_loop
sequence annotation: -35
sequence annotation: conserved
sequence annotation: polya
sequence annotation: start
sequence annotation: BBa_B0015
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010 w/o stop
sequence annotation: LVA-ssrA tag
sequence annotation: BBa_B0034
sequence annotation: CAP binding site
sequence: https://synbiohub.org/public/igem/BBa_K1399011_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2386303
display id: annotation2386303
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386303/range2386303/1

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type: Range
persistent identity: range2386303
display id: range2386303
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 938
end: 940
orientation: inline

Barcode of BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386304
display id: annotation2386304
version: 1
top level: BBa_K1399011
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386304/range2386304/1

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type: Range
persistent identity: range2386304
display id: range2386304
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 941
end: 965
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2386308
display id: annotation2386308
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386308/range2386308/1
type: Range
persistent identity: range2386308
display id: range2386308
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1062
end: 1102
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386311
display id: annotation2386311
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386311/range2386311/1

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type: Range
persistent identity: range2386311
display id: range2386311
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1095
end: 1095
orientation: inline

BBa_K1399001
type: SequenceAnnotation
persistent identity: annotation2386299
display id: annotation2386299
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386299/range2386299/1
type: Range
persistent identity: range2386299
display id: range2386299
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 227
end: 940
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2386293
display id: annotation2386293
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386293/range2386293/1
type: Range
persistent identity: range2386293
display id: range2386293
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 161
end: 166
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2386290
display id: annotation2386290
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386290/range2386290/1
type: Range
persistent identity: range2386290
display id: range2386290
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 88
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2386309
display id: annotation2386309
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386309/range2386309/1
type: Range
persistent identity: range2386309
display id: range2386309
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1069
end: 1088
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2386294
display id: annotation2386294
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386294/range2386294/1
type: Range
persistent identity: range2386294
display id: range2386294
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 166
end: 200
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2386307
display id: annotation2386307
version: 1
top level: BBa_K1399011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386307/range2386307/1
type: Range
persistent identity: range2386307
display id: range2386307
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 985
end: 1028
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2386292
display id: annotation2386292
version: 1
top level: BBa_K1399011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386292/range2386292/1
type: Range
persistent identity: range2386292
display id: range2386292
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 137
end: 142
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2386297
display id: annotation2386297
version: 1
top level: BBa_K1399011
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386297/range2386297/1
type: Range
persistent identity: range2386297
display id: range2386297
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 213
end: 216
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2386310
display id: annotation2386310
version: 1
top level: BBa_K1399011
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386310/range2386310/1
type: Range
persistent identity: range2386310
display id: range2386310
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1089
end: 1102
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386295
display id: annotation2386295
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386295/range2386295/1
type: Range
persistent identity: range2386295
display id: range2386295
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 173
end: 173
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2386305
display id: annotation2386305
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386305/range2386305/1
type: Range
persistent identity: range2386305
display id: range2386305
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 974
end: 1102
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2386289
display id: annotation2386289
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386289/range2386289/1
type: Range
persistent identity: range2386289
display id: range2386289
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2386306
display id: annotation2386306
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386306/range2386306/1
type: Range
persistent identity: range2386306
display id: range2386306
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 974
end: 1053
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2386300
display id: annotation2386300
version: 1
top level: BBa_K1399011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386300/range2386300/1
type: Range
persistent identity: range2386300
display id: range2386300
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 230
end: 901
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2386298
display id: annotation2386298
version: 1
top level: BBa_K1399011
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386298/range2386298/1
type: Range
persistent identity: range2386298
display id: range2386298
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 227
end: 229
orientation: inline

LVA-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2386301
display id: annotation2386301
version: 1
top level: BBa_K1399011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386301/range2386301/1
type: Range
persistent identity: range2386301
display id: range2386301
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 902
end: 934
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2386302
display id: annotation2386302
version: 1
top level: BBa_K1399011
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386302/range2386302/1
type: Range
persistent identity: range2386302
display id: range2386302
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 935
end: 937
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2386296
display id: annotation2386296
version: 1
top level: BBa_K1399011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386296/range2386296/1
type: Range
persistent identity: range2386296
display id: range2386296
version: 1
direction: direction/reverse
top level: BBa_K1399011
start: 209
end: 220
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2386291
display id: annotation2386291
version: 1
top level: BBa_K1399011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1

https://synbiohub.org/public/igem/BBa_K1399011/annotation2386291/range2386291/1
type: Range
persistent identity: range2386291
display id: range2386291
version: 1
direction: direction/forward
top level: BBa_K1399011
start: 89
end: 126
orientation: inline

BBa_K1415109
type: ComponentDefinition
persistent identity: BBa K1415109
display id: BBa_K1415109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons+B0034+PBAN(Aedes aegypti)
creator: HO, TSUNG YU
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has a constitutive promoter, so our PBAN( Aedes aegypti) can constitutive express.
mutable notes: No
mutable provenance: Assemble
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23101
component: BBa_K1415009
component: BBa_B0034
sequence annotation: BBa_K1415009
sequence annotation: BBa_J23101
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1415109_sequence/1

BBa_J23101
type: Component
persistent identity: component2394000
display id: component2394000
version: 1
top level: BBa_K1415109
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K1415009
type: Component
persistent identity: component2394004
display id: component2394004
version: 1
top level: BBa_K1415109
access: public
definition: BBa_K1415009

BBa_B0034
type: Component
persistent identity: component2394002
display id: component2394002
version: 1
top level: BBa_K1415109
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1415009
type: SequenceAnnotation
persistent identity: annotation2394004
display id: annotation2394004
version: 1
top level: BBa_K1415109
location: https://synbiohub.org/public/igem/BBa_K1415109/annotation2394004/range2394004/1
component: BBa_K1415009

https://synbiohub.org/public/igem/BBa_K1415109/annotation2394004/range2394004/1
type: Range
persistent identity: range2394004
display id: range2394004
version: 1
direction: direction/reverse
top level: BBa_K1415109
start: 62
end: 125
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2394000
display id: annotation2394000
version: 1
top level: BBa_K1415109
location: https://synbiohub.org/public/igem/BBa_K1415109/annotation2394000/range2394000/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1415109/annotation2394000/range2394000/1
type: Range
persistent identity: range2394000
display id: range2394000
version: 1
direction: direction/reverse
top level: BBa_K1415109
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2394002
display id: annotation2394002
version: 1
top level: BBa_K1415109
location: https://synbiohub.org/public/igem/BBa_K1415109/annotation2394002/range2394002/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1415109/annotation2394002/range2394002/1
type: Range
persistent identity: range2394002
display id: range2394002
version: 1
direction: direction/reverse
top level: BBa_K1415109
start: 44
end: 55
orientation: inline

BBa_K1493802
type: ComponentDefinition
persistent identity: BBa K1493802
display id: BBa_K1493802
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with Tet operator site
creator: Max van der Ploeg
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR operator site
sequence annotation: promoter
sequence annotation: -10 binding site
sequence annotation: -34 binding site
sequence: https://synbiohub.org/public/igem/BBa_K1493802_sequence/1

TetR operator site
type: SequenceAnnotation
persistent identity: annotation2422401
display id: annotation2422401
version: 1
top level: BBa_K1493802
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493802/annotation2422401/range2422401/1

https://synbiohub.org/public/igem/BBa_K1493802/annotation2422401/range2422401/1
type: Range
persistent identity: range2422401
display id: range2422401
version: 1
direction: direction/forward
top level: BBa_K1493802
start: 20
end: 37
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2422399
display id: annotation2422399
version: 1
top level: BBa_K1493802
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1493802/annotation2422399/range2422399/1

https://synbiohub.org/public/igem/BBa_K1493802/annotation2422399/range2422399/1
type: Range
persistent identity: range2422399
display id: range2422399
version: 1
direction: direction/forward
top level: BBa_K1493802
start: 1
end: 103
orientation: inline

-10 binding site
type: SequenceAnnotation
persistent identity: annotation2422443
display id: annotation2422443
version: 1
top level: BBa_K1493802
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1493802/annotation2422443/range2422443/1

https://synbiohub.org/public/igem/BBa_K1493802/annotation2422443/range2422443/1
type: Range
persistent identity: range2422443
display id: range2422443
version: 1
direction: direction/forward
top level: BBa_K1493802
start: 60
end: 64
orientation: inline

-34 binding site
type: SequenceAnnotation
persistent identity: annotation2422400
display id: annotation2422400
version: 1
top level: BBa_K1493802
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1493802/annotation2422400/range2422400/1

https://synbiohub.org/public/igem/BBa_K1493802/annotation2422400/range2422400/1
type: Range
persistent identity: range2422400
display id: range2422400
version: 1
direction: direction/forward
top level: BBa_K1493802
start: 38
end: 42
orientation: inline

TetR operator site
type: SequenceAnnotation
persistent identity: annotation2422445
display id: annotation2422445
version: 1
top level: BBa_K1493802
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493802/annotation2422445/range2422445/1

https://synbiohub.org/public/igem/BBa_K1493802/annotation2422445/range2422445/1
type: Range
persistent identity: range2422445
display id: range2422445
version: 1
direction: direction/forward
top level: BBa_K1493802
start: 71
end: 87
orientation: inline

BBa_K1350005
type: ComponentDefinition
persistent identity: BBa K1350005
display id: BBa_K1350005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mdfa Antiporter
creator: Zhipeng Qiu
created: 2014-09-27T11:00:00Z
modified: 2015-05-08T01:10:01Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1725_
m user id: 0
owner id: 20480
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: MDFA is an antiporter which can make E-coli have a ability to tolerate high alkiline environment up to pH 10.
mutable notes: We use pcr to get the target genes from E-coli and invert them into pet28a which can multicopy.
mutable provenance: Escherchia coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1350005_sequence/1

N-carrier
type: ComponentDefinition
persistent identity: BBa K1362053
display id: BBa_K1362053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Carrier for RFC[105] N-terminal inserts
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22911
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_K1362426
component: BBa_K1362415
component: BBa_K1362425
component: BBa_K1362423
component: BBa_K1362440
component: BBa_K1362436
sequence annotation: BBa_K1362436
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362440
sequence annotation: BBa_K1362426
sequence annotation: BBa_K1362425
sequence annotation: BBa_K1362415
sequence: https://synbiohub.org/public/igem/BBa_K1362053_sequence/1

BBa_K1362426
type: Component
persistent identity: component2409690
display id: component2409690
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362426/1

BBa_K1362415
type: Component
persistent identity: component2409688
display id: component2409688
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362425
type: Component
persistent identity: component2409685
display id: component2409685
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362423
type: Component
persistent identity: component2409689
display id: component2409689
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362440
type: Component
persistent identity: component2409686
display id: component2409686
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362440/1

BBa_K1362436
type: Component
persistent identity: component2409687
display id: component2409687
version: 1
top level: N-carrier
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362436/1

BBa_K1362436
type: SequenceAnnotation
persistent identity: annotation2409687
display id: annotation2409687
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409687/range2409687/1
component: BBa_K1362436

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409687/range2409687/1
type: Range
persistent identity: range2409687
display id: range2409687
version: 1
direction: direction/reverse
top level: N-carrier
start: 11
end: 16
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409689
display id: annotation2409689
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409689/range2409689/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409689/range2409689/1
type: Range
persistent identity: range2409689
display id: range2409689
version: 1
direction: direction/reverse
top level: N-carrier
start: 21
end: 27
orientation: inline

BBa_K1362440
type: SequenceAnnotation
persistent identity: annotation2409686
display id: annotation2409686
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409686/range2409686/1
component: BBa_K1362440

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409686/range2409686/1
type: Range
persistent identity: range2409686
display id: range2409686
version: 1
direction: direction/reverse
top level: N-carrier
start: 7
end: 10
orientation: inline

BBa_K1362426
type: SequenceAnnotation
persistent identity: annotation2409690
display id: annotation2409690
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409690/range2409690/1
component: BBa_K1362426

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409690/range2409690/1
type: Range
persistent identity: range2409690
display id: range2409690
version: 1
direction: direction/reverse
top level: N-carrier
start: 28
end: 31
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409685
display id: annotation2409685
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409685/range2409685/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409685/range2409685/1
type: Range
persistent identity: range2409685
display id: range2409685
version: 1
direction: direction/reverse
top level: N-carrier
start: 1
end: 6
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409688
display id: annotation2409688
version: 1
top level: N-carrier
location: https://synbiohub.org/public/igem/BBa_K1362053/annotation2409688/range2409688/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362053/annotation2409688/range2409688/1
type: Range
persistent identity: range2409688
display id: range2409688
version: 1
direction: direction/reverse
top level: N-carrier
start: 17
end: 20
orientation: inline

BBa_K1424003
type: ComponentDefinition
persistent identity: BBa K1424003
display id: BBa_K1424003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kanamycin resistance for Synechocystis
creator: Jacob Lamb
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
group u list: _1802_
m user id: 0
owner id: 20667
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The kanamycin resistance gene tn903 aph from e.coli for use with Synechocystis sp. PCC 6803. The kanamycin gene to allow transformation in Synechocystis sp. PCC 6803 (Poteete et al, 2006).
mutable notes: N/A
mutable provenance: genomic DNA of kanamycin containing strain of Synechocystis sp. PCC 6803
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Synechocystis RBS + promotor region
sequence annotation: kanamucin resistance gene
sequence: https://synbiohub.org/public/igem/BBa_K1424003_sequence/1

Synechocystis RBS + promotor region
type: SequenceAnnotation
persistent identity: annotation2400731
display id: annotation2400731
version: 1
top level: BBa_K1424003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1424003/annotation2400731/range2400731/1

https://synbiohub.org/public/igem/BBa_K1424003/annotation2400731/range2400731/1
type: Range
persistent identity: range2400731
display id: range2400731
version: 1
direction: direction/forward
top level: BBa_K1424003
start: 1
end: 110
orientation: inline

kanamucin resistance gene
type: SequenceAnnotation
persistent identity: annotation2400729
display id: annotation2400729
version: 1
top level: BBa_K1424003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1424003/annotation2400729/range2400729/1

https://synbiohub.org/public/igem/BBa_K1424003/annotation2400729/range2400729/1
type: Range
persistent identity: range2400729
display id: range2400729
version: 1
direction: direction/forward
top level: BBa_K1424003
start: 120
end: 920
orientation: inline

BBa_K1499501
type: ComponentDefinition
persistent identity: BBa K1499501
display id: BBa_K1499501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Endo-1,4-beta-glucanase (cellulase gene)
creator: Jovita Byemerwa
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1879_
m user id: 0
owner id: 22072
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Endo-1,4-&-beta-glucanase (EG 1) is an enzyme that breaks down the 1,4-beta-D-glycosidic linkages in cellulose to produce glucose. The coding sequence of this gene was from Neisseria Sicca bacterium that uses cellulose acetate as its sole carbon source. This enzyme is specific for 1,4-beta linkages in cellulose.
mutable notes: Since the gene is usually secreted by the original bacterium (Neisseria Sicca), it contains a signal sequence for extra-cellular secretion. We removed this signal sequence and replaced it with a secretion tag specific for E.coli, so that the enzyme can be secreted extracellularly. In order to allow protein purification and assays, we added a His-tag at the C' terminus of the gene.
mutable provenance: Genomic sequence of Neisseria Sicca SB
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Metal-affinity tag
sequence annotation: Cellulase
sequence: https://synbiohub.org/public/igem/BBa_K1499501_sequence/1

Metal-affinity tag
type: SequenceAnnotation
persistent identity: annotation2430103
display id: annotation2430103
version: 1
top level: BBa_K1499501
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1499501/annotation2430103/range2430103/1

https://synbiohub.org/public/igem/BBa_K1499501/annotation2430103/range2430103/1
type: Range
persistent identity: range2430103
display id: range2430103
version: 1
direction: direction/forward
top level: BBa_K1499501
start: 1378
end: 1398
orientation: inline

Cellulase
type: SequenceAnnotation
persistent identity: annotation2412319
display id: annotation2412319
version: 1
top level: BBa_K1499501
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1499501/annotation2412319/range2412319/1

https://synbiohub.org/public/igem/BBa_K1499501/annotation2412319/range2412319/1
type: Range
persistent identity: range2412319
display id: range2412319
version: 1
direction: direction/forward
top level: BBa_K1499501
start: 1
end: 1374
orientation: inline

BBa_K1523022
type: ComponentDefinition
persistent identity: BBa K1523022
display id: BBa_K1523022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: nahE coding sequence
creator: Haotian Wang
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:50Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1905_
m user id: 0
owner id: 21246
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The gene encoding trans-o-hydroxybenzylidenepyruvate hydratase-aldolase (tHBP-HA) was isolated from Pseudomonas fluorescens N3, an environmental strain able to degrade naphthalene.
mutable provenance: Pseudomonas fluorescens (strain: PC20, biotype: F) NCBI Gene ID: 7871527
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1523022_sequence/1

BBa_K1362204
type: ComponentDefinition
persistent identity: BBa K1362204
display id: BBa_K1362204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A non-circularization construct (with His6; without RBS)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODo
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362424
component: BBa_J04450
component: BBa_K1362445
component: BBa_K1362447
component: BBa_K1362449
component: BBa_K1362459
component: BBa_J70594
component: BBa_K1362453
component: BBa_K1362458
sequence annotation: BBa_K1362458
sequence annotation: BBa_K1362453
sequence annotation: BBa_J70594
sequence annotation: BBa_K1362447
sequence annotation: BBa_K1362449
sequence annotation: BBa_K1362424
sequence annotation: BBa_K1362445
sequence annotation: BBa_K1362459
sequence annotation: BBa_J04450
sequence: https://synbiohub.org/public/igem/BBa_K1362204_sequence/1

BBa_K1362424
type: Component
persistent identity: component2410843
display id: component2410843
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362424/1

BBa_J04450
type: Component
persistent identity: component2410842
display id: component2410842
version: 1
top level: BBa_K1362204
access: public
definition: BBa_J04450

BBa_K1362445
type: Component
persistent identity: component2410820
display id: component2410820
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362445/1

BBa_K1362447
type: Component
persistent identity: component2410824
display id: component2410824
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362447/1

BBa_K1362449
type: Component
persistent identity: component2410822
display id: component2410822
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362449/1

BBa_K1362459
type: Component
persistent identity: component2410844
display id: component2410844
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362459/1

BBa_J70594
type: Component
persistent identity: component2410846
display id: component2410846
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K1362453
type: Component
persistent identity: component2410823
display id: component2410823
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362453/1

BBa_K1362458
type: Component
persistent identity: component2410821
display id: component2410821
version: 1
top level: BBa_K1362204
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362458/1

BBa_K1362458
type: SequenceAnnotation
persistent identity: annotation2410821
display id: annotation2410821
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410821/range2410821/1
component: BBa_K1362458

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410821/range2410821/1
type: Range
persistent identity: range2410821
display id: range2410821
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 13
end: 30
orientation: inline

BBa_K1362453
type: SequenceAnnotation
persistent identity: annotation2410823
display id: annotation2410823
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410823/range2410823/1
component: BBa_K1362453

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410823/range2410823/1
type: Range
persistent identity: range2410823
display id: range2410823
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 40
end: 60
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2410846
display id: annotation2410846
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410846/range2410846/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410846/range2410846/1
type: Range
persistent identity: range2410846
display id: range2410846
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 1155
end: 1160
orientation: inline

BBa_K1362447
type: SequenceAnnotation
persistent identity: annotation2410824
display id: annotation2410824
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410824/range2410824/1
component: BBa_K1362447

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410824/range2410824/1
type: Range
persistent identity: range2410824
display id: range2410824
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 61
end: 66
orientation: inline

BBa_K1362449
type: SequenceAnnotation
persistent identity: annotation2410822
display id: annotation2410822
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410822/range2410822/1
component: BBa_K1362449

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410822/range2410822/1
type: Range
persistent identity: range2410822
display id: range2410822
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 31
end: 39
orientation: inline

BBa_K1362424
type: SequenceAnnotation
persistent identity: annotation2410843
display id: annotation2410843
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410843/range2410843/1
component: BBa_K1362424

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410843/range2410843/1
type: Range
persistent identity: range2410843
display id: range2410843
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 1136
end: 1142
orientation: inline

BBa_K1362445
type: SequenceAnnotation
persistent identity: annotation2410820
display id: annotation2410820
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410820/range2410820/1
component: BBa_K1362445

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410820/range2410820/1
type: Range
persistent identity: range2410820
display id: range2410820
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 1
end: 12
orientation: inline

BBa_K1362459
type: SequenceAnnotation
persistent identity: annotation2410844
display id: annotation2410844
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410844/range2410844/1
component: BBa_K1362459

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410844/range2410844/1
type: Range
persistent identity: range2410844
display id: range2410844
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 1143
end: 1154
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2410842
display id: annotation2410842
version: 1
top level: BBa_K1362204
location: https://synbiohub.org/public/igem/BBa_K1362204/annotation2410842/range2410842/1
component: BBa_J04450

https://synbiohub.org/public/igem/BBa_K1362204/annotation2410842/range2410842/1
type: Range
persistent identity: range2410842
display id: range2410842
version: 1
direction: direction/reverse
top level: BBa_K1362204
start: 67
end: 1135
orientation: inline

BBa_K1404002
type: ComponentDefinition
persistent identity: BBa K1404002
display id: BBa_K1404002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptac-NiCoTB, improves nickel and cobalt internalization
creator: Alexandre Duprey
created: 2014-09-22T11:00:00Z
modified: 2015-07-23T03:01:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 4206
owner id: 8419
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part comprises a strong E. coli promoter with...
mutable notes: Temp
mutable provenance: The promoter and the rest...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: B0014 terminator
sequence annotation: Ptac
sequence annotation: NiCoT
sequence annotation: RBS Paris 2009
sequence: https://synbiohub.org/public/igem/BBa_K1404002_sequence/1

B0014 terminator
type: SequenceAnnotation
persistent identity: annotation2385408
display id: annotation2385408
version: 1
top level: BBa_K1404002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385408/range2385408/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385408/range2385408/1
type: Range
persistent identity: range2385408
display id: range2385408
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 1212
end: 1306
orientation: inline

Ptac
type: SequenceAnnotation
persistent identity: annotation2385405
display id: annotation2385405
version: 1
top level: BBa_K1404002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385405/range2385405/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385405/range2385405/1
type: Range
persistent identity: range2385405
display id: range2385405
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 1
end: 76
orientation: inline

NiCoT
type: SequenceAnnotation
persistent identity: annotation2385406
display id: annotation2385406
version: 1
top level: BBa_K1404002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385406/range2385406/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385406/range2385406/1
type: Range
persistent identity: range2385406
display id: range2385406
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 96
end: 1212
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2385407
display id: annotation2385407
version: 1
top level: BBa_K1404002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404002/annotation2385407/range2385407/1

https://synbiohub.org/public/igem/BBa_K1404002/annotation2385407/range2385407/1
type: Range
persistent identity: range2385407
display id: range2385407
version: 1
direction: direction/forward
top level: BBa_K1404002
start: 83
end: 95
orientation: inline

BBa_K1351005
type: ComponentDefinition
persistent identity: BBa K1351005
display id: BBa_K1351005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SigD-dependent flagellin promoter (Phag) of Bacillus subtilis
creator: Mona Dotzler
created: 2014-10-04T11:00:00Z
modified: 2015-06-01T08:15:22Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phag promoter of ''B. subtilis'' flagellin under the control of SigD.
mutable notes: to be filled
mutable provenance: This part was generated by amplification from ''B. subtilis'' W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3. Phag_ENX_fwd: TGCAGAATTCGCGGCCGCTTCTAGAG ttcacaggaaagaaatttacttgacc Phag_SNP_rev: AGCCTGCAGCGGCCGCTACTAGTA gaatatgttgttaaggcacgtcc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: transcription start
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K1351005_sequence/1

transcription start
type: SequenceAnnotation
persistent identity: annotation2394212
display id: annotation2394212
version: 1
top level: BBa_K1351005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1351005/annotation2394212/range2394212/1

https://synbiohub.org/public/igem/BBa_K1351005/annotation2394212/range2394212/1
type: Range
persistent identity: range2394212
display id: range2394212
version: 1
direction: direction/forward
top level: BBa_K1351005
start: 154
end: 155
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2394210
display id: annotation2394210
version: 1
top level: BBa_K1351005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1351005/annotation2394210/range2394210/1

https://synbiohub.org/public/igem/BBa_K1351005/annotation2394210/range2394210/1
type: Range
persistent identity: range2394210
display id: range2394210
version: 1
direction: direction/forward
top level: BBa_K1351005
start: 121
end: 124
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2394211
display id: annotation2394211
version: 1
top level: BBa_K1351005
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1351005/annotation2394211/range2394211/1

https://synbiohub.org/public/igem/BBa_K1351005/annotation2394211/range2394211/1
type: Range
persistent identity: range2394211
display id: range2394211
version: 1
direction: direction/forward
top level: BBa_K1351005
start: 140
end: 147
orientation: inline

BBa_K1351003
type: ComponentDefinition
persistent identity: BBa K1351003
display id: BBa_K1351003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Laminin-derived peptide binding to NADH Oxidase of S. pneumoniae
creator: Mona Dotzler
created: 2014-10-02T11:00:00Z
modified: 2015-06-01T12:53:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Short peptide of 9 amino acids corresponding to amino acids 661 to 668 of the human protein Laminin (subunit alpha-5, UniProt [http://www.uniprot.org/uniprot/O15230 O15230]). It has been shown to bind to NADH oxidase (NOX) of ''Streptococcus pneumoniae'' and inhibit bacterial binding to human A549 cells (Muchnik ''et. al.'', 2013). Displayed on the surface of ''Bacillus subtilis'', it should enable ''B. subtilis'' to bind specifically to ''S. pneumoniae''. This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
mutable notes: For generation of the part's nucleotide sequence, the amino acid sequence of the peptide (Muchnik et. al., 2013) was reverse transcribed with regard to the codon usage of Bacillus subtilis. Oligos were designed as precut by EcoRI and PstI to shorten the synthesized fragments and to simplify the cloning into pSB1C3.
mutable provenance: The part was generated by annealing of artificially synthesized oligonucletides: L5P_ENX_SD_Ngo_precutEP_fwd: AATTCgcggccgctTCTAGAgtaaggaggaaGCCGGCATG TGTCAAGAATGTTCACCGGGATTT ACCGGTtaatACTAGTagcggccgCTGCA L5P_SNP_Age_precutEP_rev: GcggccgctACTAGTattaACCGGT AAATCCCGGTGAACATTCTTGACA CATGCCGGCttcctccttacTCTAGAagcggccgcG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351003_sequence/1

BBa_K1460003
type: ComponentDefinition
persistent identity: BBa K1460003
display id: BBa_K1460003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter + NixA + Ter
creator: Eric Holmes
created: 2014-08-13T11:00:00Z
modified: 2016-01-13T10:21:44Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a functional coding sequence for the protein NixA, which is a nickel transporter from H. pylori. This part will result in nickel intolerance from higher levels of intracellular nickel.
mutable notes: The Anderson promoter provides strong constitutive expression of NixA in E. coli.
mutable provenance: Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in H. pylori. This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J23102
sequence annotation: BBa_B1006
sequence annotation: NixA
sequence annotation: BBa_J61101
sequence: https://synbiohub.org/public/igem/BBa_K1460003_sequence/1

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2381013
display id: annotation2381013
version: 1
top level: BBa_K1460003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381013/range2381013/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381013/range2381013/1
type: Range
persistent identity: range2381013
display id: range2381013
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 1
end: 35
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2381016
display id: annotation2381016
version: 1
top level: BBa_K1460003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381016/range2381016/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381016/range2381016/1
type: Range
persistent identity: range2381016
display id: range2381016
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 990
end: 1028
orientation: inline

NixA
type: SequenceAnnotation
persistent identity: annotation2381015
display id: annotation2381015
version: 1
top level: BBa_K1460003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381015/range2381015/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381015/range2381015/1
type: Range
persistent identity: range2381015
display id: range2381015
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 63
end: 989
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2381014
display id: annotation2381014
version: 1
top level: BBa_K1460003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460003/annotation2381014/range2381014/1

https://synbiohub.org/public/igem/BBa_K1460003/annotation2381014/range2381014/1
type: Range
persistent identity: range2381014
display id: range2381014
version: 1
direction: direction/forward
top level: BBa_K1460003
start: 43
end: 56
orientation: inline

BBa_K1321005
type: ComponentDefinition
persistent identity: BBa K1321005
display id: BBa_K1321005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Synthetic Phytochelatin EC(20) RFC[25]
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Synthetic Phytochelatin EC(20) rfc25 fusion format
mutable notes: Codon optimised for E.coli.
mutable provenance: Sequence is not natural. Basis for the sequence was from the following reference: Bae W, Chen W, Mulchandani A, Mehra RK (2000) Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins. Biotechnol Bioeng. 70(5):518-24.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Synthetic Phytochelatin EC(20)
sequence: https://synbiohub.org/public/igem/BBa_K1321005_sequence/1

Synthetic Phytochelatin EC(20)
type: SequenceAnnotation
persistent identity: annotation2414598
display id: annotation2414598
version: 1
top level: BBa_K1321005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321005/annotation2414598/range2414598/1

https://synbiohub.org/public/igem/BBa_K1321005/annotation2414598/range2414598/1
type: Range
persistent identity: range2414598
display id: range2414598
version: 1
direction: direction/forward
top level: BBa_K1321005
start: 1
end: 123
orientation: inline

BBa_K1333307
type: ComponentDefinition
persistent identity: BBa K1333307
display id: BBa_K1333307
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13453-PrfA-PⅧ
creator: Runwen Yao
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15873
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1333005
component: BBa_K115003
component: BBa_I13453
sequence annotation: BBa_K1333005
sequence annotation: BBa_K115003
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1333307_sequence/1

BBa_K1333005
type: Component
persistent identity: component2386240
display id: component2386240
version: 1
top level: BBa_K1333307
access: public
definition: BBa_K1333005

BBa_K115003
type: Component
persistent identity: component2386237
display id: component2386237
version: 1
top level: BBa_K1333307
access: public
definition: https://synbiohub.org/public/igem/BBa_K115003/1

BBa_I13453
type: Component
persistent identity: component2386234
display id: component2386234
version: 1
top level: BBa_K1333307
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K1333005
type: SequenceAnnotation
persistent identity: annotation2386240
display id: annotation2386240
version: 1
top level: BBa_K1333307
location: https://synbiohub.org/public/igem/BBa_K1333307/annotation2386240/range2386240/1
component: BBa_K1333005

https://synbiohub.org/public/igem/BBa_K1333307/annotation2386240/range2386240/1
type: Range
persistent identity: range2386240
display id: range2386240
version: 1
direction: direction/reverse
top level: BBa_K1333307
start: 254
end: 508
orientation: inline

BBa_K115003
type: SequenceAnnotation
persistent identity: annotation2386237
display id: annotation2386237
version: 1
top level: BBa_K1333307
location: https://synbiohub.org/public/igem/BBa_K1333307/annotation2386237/range2386237/1
component: BBa_K115003

https://synbiohub.org/public/igem/BBa_K1333307/annotation2386237/range2386237/1
type: Range
persistent identity: range2386237
display id: range2386237
version: 1
direction: direction/reverse
top level: BBa_K1333307
start: 139
end: 247
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2386234
display id: annotation2386234
version: 1
top level: BBa_K1333307
location: https://synbiohub.org/public/igem/BBa_K1333307/annotation2386234/range2386234/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1333307/annotation2386234/range2386234/1
type: Range
persistent identity: range2386234
display id: range2386234
version: 1
direction: direction/reverse
top level: BBa_K1333307
start: 1
end: 130
orientation: inline

BBa_K1333318
type: ComponentDefinition
persistent identity: BBa K1333318
display id: BBa_K1333318
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PIII with an RNA thermometer(Rose42)
creator: Wei Tang
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15896
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: We add an araBAD promoter up to this part to regulate the PIII; expression wtih ara and temperature.
mutable notes: To regulate PIII expression with temperature on the translation level used RNAT.
mutable provenance: The RNAT(Rose42) comes from bacteria genome and PIII; comes from M13 phage genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K115001
component: BBa_K1333002
sequence annotation: BBa_K1333002
sequence annotation: BBa_K115001
sequence: https://synbiohub.org/public/igem/BBa_K1333318_sequence/1

BBa_K115001
type: Component
persistent identity: component2386144
display id: component2386144
version: 1
top level: BBa_K1333318
access: public
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display id: component2386148
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1333002/1

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component: BBa_K115001

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BBa_K1491009
type: ComponentDefinition
persistent identity: BBa K1491009
display id: BBa_K1491009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-mRFP-His
creator: Danielle Peters
created: 2014-07-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP with 6x His tag (BBa_K1491008) and a strong RBS (BBa_B0034). It can be used as a reporter.
mutable notes: RBS+RFP+His
mutable provenance: RBS is BBa_B0034 and mRFP is BBa_E1010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1491008
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1491008
sequence: https://synbiohub.org/public/igem/BBa_K1491009_sequence/1

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access: public
definition: https://synbiohub.org/public/igem/BBa_K1491008/1

BBa_B0034
type: Component
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display id: component2380111
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

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display id: annotation2380111
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component: BBa_B0034

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BBa_K1362021
type: ComponentDefinition
persistent identity: BBa K1362021
display id: BBa_K1362021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Circular Xylanase with His6 linker
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22920
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1362443
component: BBa_K1362419
component: BBa_K1362434
component: BBa_K1362999
component: BBa_K1362415
component: BBa_K1362090
component: BBa_K1362400
component: BBa_K1362433
component: BBa_K1362401
component: BBa_K1362421
component: BBa_G0000
component: BBa_K1362441
component: BBa_K1362414
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sequence annotation: BBa_K1362401
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sequence annotation: BBa_K1362443
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sequence annotation: BBa_K1362433
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sequence: https://synbiohub.org/public/igem/BBa_K1362021_sequence/1

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definition: https://synbiohub.org/public/igem/BBa_K1362419/1

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definition: https://synbiohub.org/public/igem/BBa_K1362400/1

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persistent identity: range2409607
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component: BBa_G0000

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persistent identity: range2409595
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display id: annotation2409602
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BBa_K1325001
type: ComponentDefinition
persistent identity: BBa K1325001
display id: BBa_K1325001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ranscriptionfactor
creator: Ke Xu
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
group u list: _1700_
m user id: 0
owner id: 23253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ranscriptionfactor，the ipgC???mxiE complex activates transcriptionfrom the pipaH* promoter
mutable notes: no
mutable provenance: gene clusters encoding type III secretion systems
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1325001_sequence/1

BBa_K1362173
type: ComponentDefinition
persistent identity: BBa K1362173
display id: BBa_K1362173
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split sfGFP reconstitution N-terminal splicing half
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J18909
component: BBa_K1362475
component: BBa_G0000
component: BBa_K1362471
component: BBa_J70594
component: BBa_K1362090
component: BBa_K1362400
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sequence annotation: BBa_J70594
sequence annotation: BBa_J18909
sequence: https://synbiohub.org/public/igem/BBa_K1362173_sequence/1

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BBa_G0000
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display id: component2414345
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definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362471
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display id: component2414349
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definition: https://synbiohub.org/public/igem/BBa_K1362471/1

BBa_J70594
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display id: component2414355
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definition: https://synbiohub.org/public/igem/BBa_J70594/1

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display id: component2414351
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1362400/1

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orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2414353
display id: annotation2414353
version: 1
top level: BBa_K1362173
location: https://synbiohub.org/public/igem/BBa_K1362173/annotation2414353/range2414353/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_K1362173/annotation2414353/range2414353/1
type: Range
persistent identity: range2414353
display id: range2414353
version: 1
direction: direction/reverse
top level: BBa_K1362173
start: 552
end: 569
orientation: inline

BBa_K1510003
type: ComponentDefinition
persistent identity: BBa K1510003
display id: BBa_K1510003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcon (S.mutans)
creator: Yen-An Chang
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:47Z
discontinued: false
dominant: false
group u list: _1890_
m user id: 0
owner id: 21272
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is derived from Streptococcus mutans UA159. The main function of this promoter is to trigger downstream gene continually.
mutable notes: In order to maintain the complete function of this promoter, RBS is retained when amplifying the sequence with PCR. Consequently, the part consists of one constitutive promoter and RBS.
mutable provenance: Streptococcus mutans UA159
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1510003_sequence/1

BBa_K1362055
type: ComponentDefinition
persistent identity: BBa K1362055
display id: BBa_K1362055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MinD MTS as RFC[105] C-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362423
component: BBa_K1362438
component: BBa_K1362426
component: BBa_K1362419
component: BBa_K1362433
component: BBa_K1362255
component: BBa_K1362425
component: BBa_K1362421
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362425
sequence annotation: BBa_K1362426
sequence annotation: BBa_K1362438
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362255
sequence annotation: BBa_K1362433
sequence: https://synbiohub.org/public/igem/BBa_K1362055_sequence/1

BBa_K1362423
type: Component
persistent identity: component2409714
display id: component2409714
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362438
type: Component
persistent identity: component2409710
display id: component2409710
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362438/1

BBa_K1362426
type: Component
persistent identity: component2409715
display id: component2409715
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362426/1

BBa_K1362419
type: Component
persistent identity: component2409707
display id: component2409707
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362433
type: Component
persistent identity: component2409709
display id: component2409709
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362433/1

BBa_K1362255
type: Component
persistent identity: component2409711
display id: component2409711
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362255/1

BBa_K1362425
type: Component
persistent identity: component2409706
display id: component2409706
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362421
type: Component
persistent identity: component2409713
display id: component2409713
version: 1
top level: BBa_K1362055
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2409707
display id: annotation2409707
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409707/range2409707/1
component: BBa_K1362419

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type: Range
persistent identity: range2409707
display id: range2409707
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 7
end: 10
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409706
display id: annotation2409706
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409706/range2409706/1
component: BBa_K1362425

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type: Range
persistent identity: range2409706
display id: range2409706
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 1
end: 6
orientation: inline

BBa_K1362426
type: SequenceAnnotation
persistent identity: annotation2409715
display id: annotation2409715
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409715/range2409715/1
component: BBa_K1362426

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type: Range
persistent identity: range2409715
display id: range2409715
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 112
end: 115
orientation: inline

BBa_K1362438
type: SequenceAnnotation
persistent identity: annotation2409710
display id: annotation2409710
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409710/range2409710/1
component: BBa_K1362438

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type: Range
persistent identity: range2409710
display id: range2409710
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 20
end: 28
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2409713
display id: annotation2409713
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409713/range2409713/1
component: BBa_K1362421

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type: Range
persistent identity: range2409713
display id: range2409713
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 101
end: 104
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409714
display id: annotation2409714
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409714/range2409714/1
component: BBa_K1362423

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type: Range
persistent identity: range2409714
display id: range2409714
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 105
end: 111
orientation: inline

BBa_K1362255
type: SequenceAnnotation
persistent identity: annotation2409711
display id: annotation2409711
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409711/range2409711/1
component: BBa_K1362255

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type: Range
persistent identity: range2409711
display id: range2409711
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 29
end: 100
orientation: inline

BBa_K1362433
type: SequenceAnnotation
persistent identity: annotation2409709
display id: annotation2409709
version: 1
top level: BBa_K1362055
location: https://synbiohub.org/public/igem/BBa_K1362055/annotation2409709/range2409709/1
component: BBa_K1362433

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type: Range
persistent identity: range2409709
display id: range2409709
version: 1
direction: direction/reverse
top level: BBa_K1362055
start: 11
end: 19
orientation: inline

BBa_K1420002
type: ComponentDefinition
persistent identity: BBa K1420002
display id: BBa_K1420002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: merB, Organomercurial Lyase from Serratia marcescens
creator: Stephen C. Heinsch
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T02:52:43Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Demethylates organomerury.
mutable notes: N/A
mutable provenance: Amplified from pDU1358, kindly donated by Anne O. Summers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merB
sequence: https://synbiohub.org/public/igem/BBa_K1420002_sequence/1

merB
type: SequenceAnnotation
persistent identity: annotation2407616
display id: annotation2407616
version: 1
top level: BBa_K1420002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420002/annotation2407616/range2407616/1

https://synbiohub.org/public/igem/BBa_K1420002/annotation2407616/range2407616/1
type: Range
persistent identity: range2407616
display id: range2407616
version: 1
direction: direction/forward
top level: BBa_K1420002
start: 1
end: 639
orientation: inline

BFRM52H
type: ComponentDefinition
persistent identity: BBa K1438001
display id: BBa_K1438001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacterioferritin (BFR) M52H heme-deletion
creator: Johann Bauerfeind
created: 2014-10-05T11:00:00Z
modified: 2015-07-22T02:13:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1816_
m user id: 4206
owner id: 19714
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ATGAAAGGTGATACTAAAGTTATAAATTATCTCAACAAACTGTTGGGAAATGAGCTTGTCGCAATCAATCAGTACTTTCTTCATGCCCGGATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGCACAAACACGCCGATCGTTATATTGAGCGCATTCTTTTTCTGGAAGGTCTTCCAAACTTACAGGACCTGGGCAAACTGAACATTGGTGAAGATGTTGAGGAAATGCTGCGTTCTGATCTGGCACTTGAGCTGGATGGCGCGAAGAATTTGCGTGAGGCAATTGGTTATGCCGATAGCGTTCATGATTACGTCAGCCGCGATATGATGATAGAAATTTTGCGCGATGAAGAAGGCCATATCGACTGGCTGGAAACGGAACTTGATCTGATTCAGAAGATGGGACTGCAAAATTATCTGCAAGCACAGATTCGCGAAGAAGGTTAA
mutable notes: The M52H Mutagensis leads to an deletion of the BFR heme residue. As a result, BFR will have a reduced iron release rate and keep it's iron content inside of the protein scaffold.
mutable provenance: Escherichia coli Nissle 1917
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1438001_sequence/1

BBa_K1362054
type: ComponentDefinition
persistent identity: BBa K1362054
display id: BBa_K1362054
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SsrA degradation tag as RFC[105] C-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22920
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362421
component: BBa_K1362425
component: BBa_K1362426
component: BBa_K1362419
component: BBa_K1362433
component: BBa_K1362423
component: BBa_K1362438
component: BBa_M0050
sequence annotation: BBa_K1362425
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362438
sequence annotation: BBa_K1362433
sequence annotation: BBa_M0050
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362426
sequence annotation: BBa_K1362419
sequence: https://synbiohub.org/public/igem/BBa_K1362054_sequence/1

BBa_K1362421
type: Component
persistent identity: component2409701
display id: component2409701
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362425
type: Component
persistent identity: component2409693
display id: component2409693
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362426
type: Component
persistent identity: component2409703
display id: component2409703
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362426/1

BBa_K1362419
type: Component
persistent identity: component2409694
display id: component2409694
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362433
type: Component
persistent identity: component2409696
display id: component2409696
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362433/1

BBa_K1362423
type: Component
persistent identity: component2409702
display id: component2409702
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362438
type: Component
persistent identity: component2409697
display id: component2409697
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362438/1

BBa_M0050
type: Component
persistent identity: component2409699
display id: component2409699
version: 1
top level: BBa_K1362054
access: public
definition: https://synbiohub.org/public/igem/BBa_M0050/1

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409693
display id: annotation2409693
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409693/range2409693/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362054/annotation2409693/range2409693/1
type: Range
persistent identity: range2409693
display id: range2409693
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 1
end: 6
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409702
display id: annotation2409702
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409702/range2409702/1
component: BBa_K1362423

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type: Range
persistent identity: range2409702
display id: range2409702
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 66
end: 72
orientation: inline

BBa_K1362438
type: SequenceAnnotation
persistent identity: annotation2409697
display id: annotation2409697
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409697/range2409697/1
component: BBa_K1362438

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type: Range
persistent identity: range2409697
display id: range2409697
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 20
end: 28
orientation: inline

BBa_K1362433
type: SequenceAnnotation
persistent identity: annotation2409696
display id: annotation2409696
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409696/range2409696/1
component: BBa_K1362433

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type: Range
persistent identity: range2409696
display id: range2409696
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 11
end: 19
orientation: inline

BBa_M0050
type: SequenceAnnotation
persistent identity: annotation2409699
display id: annotation2409699
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409699/range2409699/1
component: BBa_M0050

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type: Range
persistent identity: range2409699
display id: range2409699
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 29
end: 61
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2409701
display id: annotation2409701
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409701/range2409701/1
component: BBa_K1362421

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type: Range
persistent identity: range2409701
display id: range2409701
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 62
end: 65
orientation: inline

BBa_K1362426
type: SequenceAnnotation
persistent identity: annotation2409703
display id: annotation2409703
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409703/range2409703/1
component: BBa_K1362426

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type: Range
persistent identity: range2409703
display id: range2409703
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 73
end: 76
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2409694
display id: annotation2409694
version: 1
top level: BBa_K1362054
location: https://synbiohub.org/public/igem/BBa_K1362054/annotation2409694/range2409694/1
component: BBa_K1362419

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type: Range
persistent identity: range2409694
display id: range2409694
version: 1
direction: direction/reverse
top level: BBa_K1362054
start: 7
end: 10
orientation: inline

BBa_K1355004
type: ComponentDefinition
persistent identity: BBa K1355004
display id: BBa_K1355004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury ions bioremediating device
creator: Luna Barroco de Lacerda, Maria Clara Tavares Astolfi
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 0
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The biorremediator device is composed by our ???essential biobrick??? (BBa_K1355001) + a MerA gene (BBa_K1355000). In the presence of mercury, The MerR protein will bind to it and MerT, MerP and MerA protein will be expressed and bioremediation will start! The MerP and MerT proteins are responsible for the transport of mercury from the periplasm to the cytoplasm, leading him to the mercury ion reductase (MerA)! The beautiful MerA be responsible for the reduction from Hg2+ in Hg0, which is volatile at room temperature and able to passively leave out the bacteria.
mutable notes: For this construct, we followed these steps: Extraction of plasmid DNA and DNA quantification of bacteria transformed with the Essential Biobrick (BBa_K1355001) and the bacteria transformed with the MerA Biobrick (BBa_E0840); Verifying the electrophoretic profile of the extracted plasmid DNA; Restriction enzyme digestion for Essential Biobrick with SpeI and PstI and for merA Biobrick with PstI and XbaI; Checking the electrophoretic profile of digested samples; Fragment of interest purification from the gel; Ligation the gel purification of merA gene and digested Essential Biobrick; Transformation of ligation in DH5-alpha; Plasmid DNA extraction from bacteria transformed; Check the electrophoretic profile to see results of samples linked (no fragments); Digestion of Essential Biobrick + merA Biobrick with EcoRI and PstI, aiming to analyze the fragment size to be isolated; Checking the electrophoretic profile of the digested sample to obtain results showing that the isolated fragment is the junction of Essential Biobrick +merA Biobrick ;
mutable provenance: BBa_K1355001, BBa_K1355000
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1355000
component: BBa_K1355001
sequence annotation: BBa_K1355000
sequence annotation: BBa_K1355001
sequence: https://synbiohub.org/public/igem/BBa_K1355004_sequence/1

BBa_K1355000
type: Component
persistent identity: component2415200
display id: component2415200
version: 1
top level: BBa_K1355004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1355000/1

BBa_K1355001
type: Component
persistent identity: component2415196
display id: component2415196
version: 1
top level: BBa_K1355004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1355001/1

BBa_K1355000
type: SequenceAnnotation
persistent identity: annotation2415200
display id: annotation2415200
version: 1
top level: BBa_K1355004
location: https://synbiohub.org/public/igem/BBa_K1355004/annotation2415200/range2415200/1
component: BBa_K1355000

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type: Range
persistent identity: range2415200
display id: range2415200
version: 1
direction: direction/reverse
top level: BBa_K1355004
start: 1198
end: 2975
orientation: inline

BBa_K1355001
type: SequenceAnnotation
persistent identity: annotation2415196
display id: annotation2415196
version: 1
top level: BBa_K1355004
location: https://synbiohub.org/public/igem/BBa_K1355004/annotation2415196/range2415196/1
component: BBa_K1355001

https://synbiohub.org/public/igem/BBa_K1355004/annotation2415196/range2415196/1
type: Range
persistent identity: range2415196
display id: range2415196
version: 1
direction: direction/reverse
top level: BBa_K1355004
start: 1
end: 1189
orientation: inline

BBa_K1493801
type: ComponentDefinition
persistent identity: BBa K1493801
display id: BBa_K1493801
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter with Tet operator site
creator: Max van der Ploeg
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: promoter
sequence annotation: tetR operator site
sequence annotation: -10 binding site
sequence annotation: -34 binding site
sequence: https://synbiohub.org/public/igem/BBa_K1493801_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2407305
display id: annotation2407305
version: 1
top level: BBa_K1493801
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1493801/annotation2407305/range2407305/1

https://synbiohub.org/public/igem/BBa_K1493801/annotation2407305/range2407305/1
type: Range
persistent identity: range2407305
display id: range2407305
version: 1
direction: direction/forward
top level: BBa_K1493801
start: 1
end: 103
orientation: inline

tetR operator site
type: SequenceAnnotation
persistent identity: annotation2407306
display id: annotation2407306
version: 1
top level: BBa_K1493801
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493801/annotation2407306/range2407306/1

https://synbiohub.org/public/igem/BBa_K1493801/annotation2407306/range2407306/1
type: Range
persistent identity: range2407306
display id: range2407306
version: 1
direction: direction/forward
top level: BBa_K1493801
start: 49
end: 67
orientation: inline

-10 binding site
type: SequenceAnnotation
persistent identity: annotation2407314
display id: annotation2407314
version: 1
top level: BBa_K1493801
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1493801/annotation2407314/range2407314/1

https://synbiohub.org/public/igem/BBa_K1493801/annotation2407314/range2407314/1
type: Range
persistent identity: range2407314
display id: range2407314
version: 1
direction: direction/forward
top level: BBa_K1493801
start: 62
end: 67
orientation: inline

-34 binding site
type: SequenceAnnotation
persistent identity: annotation2407312
display id: annotation2407312
version: 1
top level: BBa_K1493801
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1493801/annotation2407312/range2407312/1

https://synbiohub.org/public/igem/BBa_K1493801/annotation2407312/range2407312/1
type: Range
persistent identity: range2407312
display id: range2407312
version: 1
direction: direction/forward
top level: BBa_K1493801
start: 39
end: 44
orientation: inline

tetR operator site
type: SequenceAnnotation
persistent identity: annotation2407310
display id: annotation2407310
version: 1
top level: BBa_K1493801
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K1493801/annotation2407310/range2407310/1

https://synbiohub.org/public/igem/BBa_K1493801/annotation2407310/range2407310/1
type: Range
persistent identity: range2407310
display id: range2407310
version: 1
direction: direction/forward
top level: BBa_K1493801
start: 73
end: 90
orientation: inline

BBa_K1315008
type: ComponentDefinition
persistent identity: BBa K1315008
display id: BBa_K1315008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The cblD promoter from Burkholderia
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a promoter that is activated by the OmpR-like two-component response regulator BCAM0228 (Bba_K1315007) in the BCAM0227-BCAM0228 sensor system. The Burkholderia diffusible signal (BDSF) is being picked up by the BCAM0227 sensor kinase that is hypothesised to eventually lead in involvement of BCAM0228. BCAM0228 is then activating cblD.
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector
mutable provenance: The source of this part is Burkholderia cenocepacia J2315 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cblD
sequence: https://synbiohub.org/public/igem/BBa_K1315008_sequence/1

cblD
type: SequenceAnnotation
persistent identity: annotation2387049
display id: annotation2387049
version: 1
top level: BBa_K1315008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1315008/annotation2387049/range2387049/1

https://synbiohub.org/public/igem/BBa_K1315008/annotation2387049/range2387049/1
type: Range
persistent identity: range2387049
display id: range2387049
version: 1
direction: direction/forward
top level: BBa_K1315008
start: 1
end: 250
orientation: inline

BBa_K1399012
type: ComponentDefinition
persistent identity: BBa K1399012
display id: BBa_K1399012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(LAA)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RBS, RFP reporter tagged with LAA-ssrA degradation tag and terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: start
sequence annotation: barcode from BBa_E1010
sequence annotation: stop
sequence annotation: CAP binding site
sequence annotation: -10
sequence annotation: end of LacI coding region (inactive)
sequence annotation: LacI binding site
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: polya
sequence annotation: BBa_B0010
sequence annotation: T7 TE
sequence annotation: -35
sequence annotation: conserved
sequence annotation: BBa_B0012
sequence annotation: tag
sequence annotation: BBa_B0034
sequence annotation: stem_loop
sequence annotation: BBa_K1399002
sequence annotation: RFP
sequence: https://synbiohub.org/public/igem/BBa_K1399012_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2383992
display id: annotation2383992
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383992/range2383992/1
type: Range
persistent identity: range2383992
display id: range2383992
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 173
end: 173
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386312
display id: annotation2386312
version: 1
top level: BBa_K1399012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2386312/range2386312/1
type: Range
persistent identity: range2386312
display id: range2386312
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 941
end: 965
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384006
display id: annotation2384006
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384006/range2384006/1
type: Range
persistent identity: range2384006
display id: range2384006
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1095
end: 1095
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383988
display id: annotation2383988
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383988/range2383988/1
type: Range
persistent identity: range2383988
display id: range2383988
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 89
end: 126
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383990
display id: annotation2383990
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383990/range2383990/1
type: Range
persistent identity: range2383990
display id: range2383990
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 161
end: 166
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383998
display id: annotation2383998
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383998/range2383998/1
type: Range
persistent identity: range2383998
display id: range2383998
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 935
end: 937
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383987
display id: annotation2383987
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383987/range2383987/1
type: Range
persistent identity: range2383987
display id: range2383987
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 88
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383991
display id: annotation2383991
version: 1
top level: BBa_K1399012
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383991/range2383991/1
type: Range
persistent identity: range2383991
display id: range2383991
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 166
end: 200
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383986
display id: annotation2383986
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383986/range2383986/1
type: Range
persistent identity: range2383986
display id: range2383986
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1
end: 200
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384007
display id: annotation2384007
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384007/range2384007/1
type: Range
persistent identity: range2384007
display id: range2384007
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 974
end: 1102
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384005
display id: annotation2384005
version: 1
top level: BBa_K1399012
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384005/range2384005/1
type: Range
persistent identity: range2384005
display id: range2384005
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1089
end: 1102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384001
display id: annotation2384001
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384001/range2384001/1
type: Range
persistent identity: range2384001
display id: range2384001
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 974
end: 1053
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384004
display id: annotation2384004
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384004/range2384004/1
type: Range
persistent identity: range2384004
display id: range2384004
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1069
end: 1088
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383989
display id: annotation2383989
version: 1
top level: BBa_K1399012
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383989/range2383989/1
type: Range
persistent identity: range2383989
display id: range2383989
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 137
end: 142
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2383993
display id: annotation2383993
version: 1
top level: BBa_K1399012
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383993/range2383993/1
type: Range
persistent identity: range2383993
display id: range2383993
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 213
end: 216
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384003
display id: annotation2384003
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384003/range2384003/1
type: Range
persistent identity: range2384003
display id: range2384003
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 1062
end: 1102
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation2383997
display id: annotation2383997
version: 1
top level: BBa_K1399012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383997/range2383997/1
type: Range
persistent identity: range2383997
display id: range2383997
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 902
end: 934
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383994
display id: annotation2383994
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383994/range2383994/1
type: Range
persistent identity: range2383994
display id: range2383994
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 209
end: 220
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383999
display id: annotation2383999
version: 1
top level: BBa_K1399012
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383999/range2383999/1
type: Range
persistent identity: range2383999
display id: range2383999
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 938
end: 940
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384002
display id: annotation2384002
version: 1
top level: BBa_K1399012
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384002/range2384002/1
type: Range
persistent identity: range2384002
display id: range2384002
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 985
end: 1028
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383995
display id: annotation2383995
version: 1
top level: BBa_K1399012
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383995/range2383995/1
type: Range
persistent identity: range2383995
display id: range2383995
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 227
end: 229
orientation: inline

BBa_K1399002
type: SequenceAnnotation
persistent identity: annotation2384000
display id: annotation2384000
version: 1
top level: BBa_K1399012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2384000/range2384000/1
type: Range
persistent identity: range2384000
display id: range2384000
version: 1
direction: direction/reverse
top level: BBa_K1399012
start: 227
end: 940
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383996
display id: annotation2383996
version: 1
top level: BBa_K1399012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1

https://synbiohub.org/public/igem/BBa_K1399012/annotation2383996/range2383996/1
type: Range
persistent identity: range2383996
display id: range2383996
version: 1
direction: direction/forward
top level: BBa_K1399012
start: 230
end: 901
orientation: inline

BBa_K1330000
type: ComponentDefinition
persistent identity: BBa K1330000
display id: BBa_K1330000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spinach2.1 flanked by tRNALys3
creator: Rasmus Bech
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1705_
m user id: 0
owner id: 20187
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Test
mutable notes: Test
mutable provenance: Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence: https://synbiohub.org/public/igem/BBa_K1330000_sequence/1

BBa_K1321009
type: ComponentDefinition
persistent identity: BBa K1321009
display id: BBa_K1321009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Nickel Binding Protein (NiBP) Freiburg format (RFC[25])
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Nickel Binding Protein in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
mutable notes: OEPCR primers were designed to allow for the replacement of the standard BioBrick prefix and suffix with the Freiburg prefix and suffix.
mutable provenance: This part is based on the existing BioBrick BBa_K1151001 by the 2013 Salente Lecce iGEM team.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Nickel Binding Protein
sequence: https://synbiohub.org/public/igem/BBa_K1321009_sequence/1

Nickel Binding Protein
type: SequenceAnnotation
persistent identity: annotation2416941
display id: annotation2416941
version: 1
top level: BBa_K1321009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321009/annotation2416941/range2416941/1

https://synbiohub.org/public/igem/BBa_K1321009/annotation2416941/range2416941/1
type: Range
persistent identity: range2416941
display id: range2416941
version: 1
direction: direction/forward
top level: BBa_K1321009
start: 1
end: 176
orientation: inline

REDOX
type: ComponentDefinition
persistent identity: BBa K1312006
display id: BBa_K1312006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NADPH-dependent flavinoid reductase relate to Met-DMSP byosynthesis pathway(REDOX)
creator: Yutaka Murata
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:09:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1687_
m user id: 0
owner id: 17633
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is NADPH-dependent flavinoid reductase gene of Fragilariopsis cylindrus. The gene code NADPH-dependent flavinoid reductase relate to Met-DMSP byosynthesis pathway from Fragilariopsis cylindrus. which catalyze second step of Met-DMSP biosynthesis pathway.
mutable notes: This part was excluded intron.
mutable provenance: From Fragilariopsis cylindrus.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: REDOX
sequence: https://synbiohub.org/public/igem/BBa_K1312006_sequence/1

REDOX
type: SequenceAnnotation
persistent identity: annotation2429347
display id: annotation2429347
version: 1
top level: REDOX
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1312006/annotation2429347/range2429347/1

https://synbiohub.org/public/igem/BBa_K1312006/annotation2429347/range2429347/1
type: Range
persistent identity: range2429347
display id: range2429347
version: 1
direction: direction/forward
top level: REDOX
start: 1
end: 705
orientation: inline

BBa_K1333201
type: ComponentDefinition
persistent identity: BBa K1333201
display id: BBa_K1333201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI-LGF2(D78A)
creator: Yanwen Xie
created: 2014-09-08T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fuse lambda cI with LGF2 mutated from Asp to Ala at site 78. As one of the components in Bacterial Two-hybrid System, it causes a less interaction with GAL11P compared to the nomalized WT.
mutable notes: Mutation of GAC to GCC LGF2 at site 78.
mutable provenance: From Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LGF2(D78A)
sequence annotation: lambda cI
sequence annotation: TGA stop codon
sequence annotation: ATG start codon
sequence: https://synbiohub.org/public/igem/BBa_K1333201_sequence/1

LGF2(D78A)
type: SequenceAnnotation
persistent identity: annotation2382832
display id: annotation2382832
version: 1
top level: BBa_K1333201
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382832/range2382832/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382832/range2382832/1
type: Range
persistent identity: range2382832
display id: range2382832
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 721
end: 840
orientation: inline

lambda cI
type: SequenceAnnotation
persistent identity: annotation2382831
display id: annotation2382831
version: 1
top level: BBa_K1333201
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382831/range2382831/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382831/range2382831/1
type: Range
persistent identity: range2382831
display id: range2382831
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 1
end: 711
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2382834
display id: annotation2382834
version: 1
top level: BBa_K1333201
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382834/range2382834/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382834/range2382834/1
type: Range
persistent identity: range2382834
display id: range2382834
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 841
end: 843
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2382833
display id: annotation2382833
version: 1
top level: BBa_K1333201
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1333201/annotation2382833/range2382833/1

https://synbiohub.org/public/igem/BBa_K1333201/annotation2382833/range2382833/1
type: Range
persistent identity: range2382833
display id: range2382833
version: 1
direction: direction/forward
top level: BBa_K1333201
start: 1
end: 3
orientation: inline

BBa_K1420000
type: ComponentDefinition
persistent identity: BBa K1420000
display id: BBa_K1420000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mer operon, biological system found to detoxify organic and inorganic forms of mercury
creator: Jessica Tarnowski
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1798_
m user id: 0
owner id: 18138
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: converts organomercuric and cationic mercury to the less toxic volatile mercury.
mutable notes: The original plasmid obtained from A. O. Summer's lab contained a the EcoRI restriction site within the operon. Mutagenesis by the UMN iGEM team removed this internal restriction site.
mutable provenance: This part was acquired courtesy of A. O. Summer's lab, in the pDU1358 plasmid.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: merR
sequence: https://synbiohub.org/public/igem/BBa_K1420000_sequence/1

merR
type: SequenceAnnotation
persistent identity: annotation2411871
display id: annotation2411871
version: 1
top level: BBa_K1420000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420000/annotation2411871/range2411871/1

https://synbiohub.org/public/igem/BBa_K1420000/annotation2411871/range2411871/1
type: Range
persistent identity: range2411871
display id: range2411871
version: 1
direction: direction/reverse
top level: BBa_K1420000
start: 1
end: 435
orientation: inline

C2HP/RGE
type: ComponentDefinition
persistent identity: BBa K1442107
display id: BBa_K1442107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C2HP Testing Module (reverse GFP)
creator: Dan Goss
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1820_
m user id: 0
owner id: 23055
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1442107_sequence/1

BBa_K1497024
type: ComponentDefinition
persistent identity: BBa K1497024
display id: BBa_K1497024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GBD-Domain
creator: Sascha Hein, Rene Sahm
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497024_sequence/1

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inducible pBad/araC promoter
creator: Sri Kosuri
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PC
sequence annotation: Pbad
sequence annotation: AraC
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
direction: direction/forward
top level: pBad/araC
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
direction: direction/forward
top level: pBad/araC
start: 1080
end: 1210
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
direction: direction/forward
top level: pBad/araC
start: 1
end: 1079
orientation: inline

BBa_K1499500
type: ComponentDefinition
persistent identity: BBa K1499500
display id: BBa_K1499500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: quorum sensing machinery that activates GFP expression
creator: Poorwa Godbole
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
group u list: _1879_
m user id: 0
owner id: 22138
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the combination of two parts, BBa_I13202 and BBa_T9002. The first part (BBa_I13202) produces a luxI enzyme under the control of a lac repressible promoter. The second part (BBa_T9002) produces luxR, which combines with the luxI to activate the promoter that controls expression of GFP.
mutable notes: N/A
mutable provenance: Combination of two previous parts.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13202
component: BBa_T9002
sequence annotation: BBa_I13202
sequence annotation: BBa_T9002
sequence: https://synbiohub.org/public/igem/BBa_K1499500_sequence/1

BBa_I13202
type: Component
persistent identity: component2381368
display id: component2381368
version: 1
top level: BBa_K1499500
access: public
definition: https://synbiohub.org/public/igem/BBa_I13202/1

BBa_T9002
type: Component
persistent identity: component2381403
display id: component2381403
version: 1
top level: BBa_K1499500
access: public
definition: https://synbiohub.org/public/igem/BBa_T9002/1

BBa_I13202
type: SequenceAnnotation
persistent identity: annotation2381368
display id: annotation2381368
version: 1
top level: BBa_K1499500
location: https://synbiohub.org/public/igem/BBa_K1499500/annotation2381368/range2381368/1
component: BBa_I13202

https://synbiohub.org/public/igem/BBa_K1499500/annotation2381368/range2381368/1
type: Range
persistent identity: range2381368
display id: range2381368
version: 1
direction: direction/reverse
top level: BBa_K1499500
start: 1
end: 803
orientation: inline

BBa_T9002
type: SequenceAnnotation
persistent identity: annotation2381403
display id: annotation2381403
version: 1
top level: BBa_K1499500
location: https://synbiohub.org/public/igem/BBa_K1499500/annotation2381403/range2381403/1
component: BBa_T9002

https://synbiohub.org/public/igem/BBa_K1499500/annotation2381403/range2381403/1
type: Range
persistent identity: range2381403
display id: range2381403
version: 1
direction: direction/reverse
top level: BBa_K1499500
start: 812
end: 2756
orientation: inline

BBa_K1321341
type: ComponentDefinition
persistent identity: BBa K1321341
display id: BBa_K1321341
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDclos in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:27:02Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdfads
mutable notes: asdfd
mutable provenance: asdfad
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321002
component: BBa_K1321337
sequence annotation: BBa_K1321002
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence: https://synbiohub.org/public/igem/BBa_K1321341_sequence/1

BBa_B0105
type: Component
persistent identity: component2415090
display id: component2415090
version: 1
top level: BBa_K1321341
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: Component
persistent identity: component2415091
display id: component2415091
version: 1
top level: BBa_K1321341
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_K1321337
type: Component
persistent identity: component2415088
display id: component2415088
version: 1
top level: BBa_K1321341
access: public
definition: BBa_K1321337

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415091
display id: annotation2415091
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415091/range2415091/1
type: Range
persistent identity: range2415091
display id: range2415091
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 718
end: 1017
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415090
display id: annotation2415090
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415090/range2415090/1
type: Range
persistent identity: range2415090
display id: range2415090
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 712
end: 717
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415088
display id: annotation2415088
version: 1
top level: BBa_K1321341
location: https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321341/annotation2415088/range2415088/1
type: Range
persistent identity: range2415088
display id: range2415088
version: 1
direction: direction/reverse
top level: BBa_K1321341
start: 1
end: 711
orientation: inline

BBa_K1497011
type: ComponentDefinition
persistent identity: BBa K1497011
display id: BBa_K1497011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-ANS
creator: Sascha Hein, Sven Jager, Thomas Dohmen
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1497011_sequence/1

BBa_K1499401
type: ComponentDefinition
persistent identity: BBa K1499401
display id: BBa_K1499401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chitin-binding wasp protein 2 (PdomMRNAr1.2-08705.1)
creator: Ian Hull
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1879_
m user id: 0
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: todo
mutable notes: todo
mutable provenance: todo
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA stop codon
sequence annotation: chitin-binding protein 2
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1499401_sequence/1

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2413318
display id: annotation2413318
version: 1
top level: BBa_K1499401
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1499401/annotation2413318/range2413318/1

https://synbiohub.org/public/igem/BBa_K1499401/annotation2413318/range2413318/1
type: Range
persistent identity: range2413318
display id: range2413318
version: 1
direction: direction/forward
top level: BBa_K1499401
start: 333
end: 335
orientation: inline

chitin-binding protein 2
type: SequenceAnnotation
persistent identity: annotation2413316
display id: annotation2413316
version: 1
top level: BBa_K1499401
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1499401/annotation2413316/range2413316/1

https://synbiohub.org/public/igem/BBa_K1499401/annotation2413316/range2413316/1
type: Range
persistent identity: range2413316
display id: range2413316
version: 1
direction: direction/forward
top level: BBa_K1499401
start: 9
end: 332
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2413317
display id: annotation2413317
version: 1
top level: BBa_K1499401
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499401/annotation2413317/range2413317/1

https://synbiohub.org/public/igem/BBa_K1499401/annotation2413317/range2413317/1
type: Range
persistent identity: range2413317
display id: range2413317
version: 1
direction: direction/forward
top level: BBa_K1499401
start: 9
end: 11
orientation: inline

BBa_K1321358
type: ComponentDefinition
persistent identity: BBa K1321358
display id: BBa_K1321358
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcenA with linker fused to NiBP driven by LacI
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:53Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20830
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321339
component: BBa_J04500
component: BBa_B0105
component: BBa_K1321009
sequence annotation: BBa_K1321009
sequence annotation: BBa_K1321339
sequence annotation: BBa_B0105
sequence annotation: BBa_J04500
sequence annotation: BBa_K1319106
sequence: https://synbiohub.org/public/igem/BBa_K1321358_sequence/1

BBa_K1319106
type: Component
persistent identity: component2422625
display id: component2422625
version: 1
top level: BBa_K1321358
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321339
type: Component
persistent identity: component2422628
display id: component2422628
version: 1
top level: BBa_K1321358
access: public
definition: BBa_K1321339

BBa_J04500
type: Component
persistent identity: component2422623
display id: component2422623
version: 1
top level: BBa_K1321358
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_B0105
type: Component
persistent identity: component2422630
display id: component2422630
version: 1
top level: BBa_K1321358
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321009
type: Component
persistent identity: component2422632
display id: component2422632
version: 1
top level: BBa_K1321358
access: public
definition: BBa_K1321009

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2422632
display id: annotation2422632
version: 1
top level: BBa_K1321358
location: https://synbiohub.org/public/igem/BBa_K1321358/annotation2422632/range2422632/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321358/annotation2422632/range2422632/1
type: Range
persistent identity: range2422632
display id: range2422632
version: 1
direction: direction/reverse
top level: BBa_K1321358
start: 650
end: 825
orientation: inline

BBa_K1321339
type: SequenceAnnotation
persistent identity: annotation2422628
display id: annotation2422628
version: 1
top level: BBa_K1321358
location: https://synbiohub.org/public/igem/BBa_K1321358/annotation2422628/range2422628/1
component: BBa_K1321339

https://synbiohub.org/public/igem/BBa_K1321358/annotation2422628/range2422628/1
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BBa_B0105
type: SequenceAnnotation
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display id: annotation2422630
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top level: BBa_K1321358
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BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2422623
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component: BBa_J04500

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type: Range
persistent identity: range2422623
display id: range2422623
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start: 1
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BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2422625
display id: annotation2422625
version: 1
top level: BBa_K1321358
location: https://synbiohub.org/public/igem/BBa_K1321358/annotation2422625/range2422625/1
component: BBa_K1319106

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type: Range
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display id: range2422625
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direction: direction/reverse
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BBa_K1497028
type: ComponentDefinition
persistent identity: BBa K1497028
display id: BBa_K1497028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold (P-P)
creator: Tobias Gabriel, Rene Sahm
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497028_sequence/1

BBa_K1362174
type: ComponentDefinition
persistent identity: BBa K1362174
display id: BBa_K1362174
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split sfGFP reconstitution N-terminal non-splicing control half
creator: Jan Gleixner
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1362090
component: BBa_J70594
component: BBa_K1362478
component: BBa_K1362471
component: BBa_J18909
component: BBa_G0000
component: BBa_K1362400
sequence annotation: BBa_K1362471
sequence annotation: BBa_G0000
sequence annotation: BBa_J18909
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362400
sequence annotation: BBa_J70594
sequence annotation: BBa_K1362478
sequence: https://synbiohub.org/public/igem/BBa_K1362174_sequence/1

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type: Component
persistent identity: component2414469
display id: component2414469
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_J70594
type: Component
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display id: component2414480
version: 1
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access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K1362478
type: Component
persistent identity: component2414475
display id: component2414475
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362478/1

BBa_K1362471
type: Component
persistent identity: component2414474
display id: component2414474
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362471/1

BBa_J18909
type: Component
persistent identity: component2414478
display id: component2414478
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_G0000
type: Component
persistent identity: component2414470
display id: component2414470
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362400
type: Component
persistent identity: component2414476
display id: component2414476
version: 1
top level: BBa_K1362174
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362400/1

BBa_K1362471
type: SequenceAnnotation
persistent identity: annotation2414474
display id: annotation2414474
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414474/range2414474/1
component: BBa_K1362471

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type: Range
persistent identity: range2414474
display id: range2414474
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start: 36
end: 245
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2414470
display id: annotation2414470
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414470/range2414470/1
component: BBa_G0000

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type: Range
persistent identity: range2414470
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version: 1
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start: 30
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BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2414478
display id: annotation2414478
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414478/range2414478/1
component: BBa_J18909

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type: Range
persistent identity: range2414478
display id: range2414478
version: 1
direction: direction/reverse
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start: 552
end: 569
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2414469
display id: annotation2414469
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414469/range2414469/1
component: BBa_K1362090

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type: Range
persistent identity: range2414469
display id: range2414469
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 1
end: 29
orientation: inline

BBa_K1362400
type: SequenceAnnotation
persistent identity: annotation2414476
display id: annotation2414476
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414476/range2414476/1
component: BBa_K1362400

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type: Range
persistent identity: range2414476
display id: range2414476
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 250
end: 551
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2414480
display id: annotation2414480
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414480/range2414480/1
component: BBa_J70594

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type: Range
persistent identity: range2414480
display id: range2414480
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 570
end: 575
orientation: inline

BBa_K1362478
type: SequenceAnnotation
persistent identity: annotation2414475
display id: annotation2414475
version: 1
top level: BBa_K1362174
location: https://synbiohub.org/public/igem/BBa_K1362174/annotation2414475/range2414475/1
component: BBa_K1362478

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type: Range
persistent identity: range2414475
display id: range2414475
version: 1
direction: direction/reverse
top level: BBa_K1362174
start: 246
end: 249
orientation: inline

BBa_K1321092
type: ComponentDefinition
persistent identity: BBa K1321092
display id: BBa_K1321092
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to CBDcex
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhytoC+CBD7. TBC
mutable notes: PhytoC+CBD7. TBC
mutable provenance: PhytoC+CBD7. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_B0105
component: BBa_K1321005
sequence annotation: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321092_sequence/1

BBa_K1321003
type: Component
persistent identity: component2414621
display id: component2414621
version: 1
top level: BBa_K1321092
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_B0105
type: Component
persistent identity: component2414620
display id: component2414620
version: 1
top level: BBa_K1321092
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: Component
persistent identity: component2414618
display id: component2414618
version: 1
top level: BBa_K1321092
access: public
definition: BBa_K1321005

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414618
display id: annotation2414618
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414618/range2414618/1
component: BBa_K1321005

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type: Range
persistent identity: range2414618
display id: range2414618
version: 1
direction: direction/reverse
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start: 1
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BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414620
display id: annotation2414620
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414620/range2414620/1
component: BBa_B0105

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type: Range
persistent identity: range2414620
display id: range2414620
version: 1
direction: direction/reverse
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start: 124
end: 129
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BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414621
display id: annotation2414621
version: 1
top level: BBa_K1321092
location: https://synbiohub.org/public/igem/BBa_K1321092/annotation2414621/range2414621/1
component: BBa_K1321003

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type: Range
persistent identity: range2414621
display id: range2414621
version: 1
direction: direction/reverse
top level: BBa_K1321092
start: 130
end: 456
orientation: inline

BBa_K1351008
type: ComponentDefinition
persistent identity: BBa K1351008
display id: BBa_K1351008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Signal peptide of B. subtilis endonuclease YhcR
creator: Mona Dotzler
created: 2014-10-04T11:00:00Z
modified: 2015-06-01T12:55:11Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Signal peptide (amino acids 1 to 46) of ''Bacillus subtilis'' endonuclease YhcR (UniProt [http://www.uniprot.org/uniprot/P54602 P54602]. YhcR is secreted and covalently anchored to the petidoglycan wall by the sortase YhcS. Despite its signal protein of RR-type, which is typical for proteins exported via the Tat pathway, export of YhcR is not Tat-dependent and seems to function via the Sec pathway (Tjalsma ''et. al.'', 2004). A YhcR-based surface display system has been shown to covalently anchor heterologous proteins to the ''B. subtilis'' cell wall (Liew ''et. al.'', 2012). It was used in the [http://2014.igem.org/Team:LMU-Munich/Project/Bakillus BaKillus project] to display pathogen-specific peptides and mediate adhesion of ''B. subtlis'' to pathogens. It consists of four components: the sortase substrate with a signal peptide (this part), a linker ([http://parts.igem.org/Part:BBa_K1351009 BBa_K1351009]) and a cell wall-anchoring domain ([http://parts.igem.org/Part:BBa_K1351010 BBa_K1351010]) as well as the sortase YhcS itself ([http://parts.igem.org/Part:BBa_K1351011 BBa_K1351011]), whose overexpression increases the efficiency of the surface display.
mutable notes: The native signal peptide of YhcR was used instead of known Sec-dependent signal peptides as Liew ''et. al.'' did 2012.
mutable provenance: This part was generated by amplification from ''B. subtilis'' W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3. SP_YhcR_ ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC ATGCTGTCTGTCGAAATGATAAGC SP_YhcR_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT AGCTTCGAACGTGTACATTACATTTAAG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351008_sequence/1

BBa_K1399002
type: ComponentDefinition
persistent identity: BBa K1399002
display id: BBa_K1399002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with LAA-ssrA degradation tag (wt)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LAA-ssrA degradation tag (part BBa_M0050). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is E. coli wild type ssrA tag and is reported to lead to very fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations; Protein stability; Km of binding to the protease; Temperature [3]. Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LAA-ssrA degradation tag (part BBa_M0050). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is E. coli wild type ssrA tag and is reported to lead to very fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease a…
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from part BBa_M0050, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tag
sequence annotation: RFP
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399002_sequence/1

tag
type: SequenceAnnotation
persistent identity: annotation2383900
display id: annotation2383900
version: 1
top level: BBa_K1399002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383900/range2383900/1

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type: Range
persistent identity: range2383900
display id: range2383900
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 676
end: 708
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383899
display id: annotation2383899
version: 1
top level: BBa_K1399002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383899/range2383899/1

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type: Range
persistent identity: range2383899
display id: range2383899
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 4
end: 675
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383901
display id: annotation2383901
version: 1
top level: BBa_K1399002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383901/range2383901/1

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type: Range
persistent identity: range2383901
display id: range2383901
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 709
end: 711
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383898
display id: annotation2383898
version: 1
top level: BBa_K1399002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383898/range2383898/1

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type: Range
persistent identity: range2383898
display id: range2383898
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383902
display id: annotation2383902
version: 1
top level: BBa_K1399002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399002/annotation2383902/range2383902/1

https://synbiohub.org/public/igem/BBa_K1399002/annotation2383902/range2383902/1
type: Range
persistent identity: range2383902
display id: range2383902
version: 1
direction: direction/forward
top level: BBa_K1399002
start: 712
end: 714
orientation: inline

BBa_K1321105
type: ComponentDefinition
persistent identity: BBa K1321105
display id: BBa_K1321105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to Phytochelatin (PC) EC20 with T7 promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD7+PhytoC
mutable notes: CBD7+PhytoC
mutable provenance: CBD7+PhytoC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321003
component: BBa_K1321338
component: BBa_K1319106
component: BBa_B0105
component: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321105_sequence/1

BBa_K1321003
type: Component
persistent identity: component2415330
display id: component2415330
version: 1
top level: BBa_K1321105
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321338
type: Component
persistent identity: component2415327
display id: component2415327
version: 1
top level: BBa_K1321105
access: public
definition: BBa_K1321338

BBa_K1319106
type: Component
persistent identity: component2415329
display id: component2415329
version: 1
top level: BBa_K1321105
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_B0105
type: Component
persistent identity: component2415332
display id: component2415332
version: 1
top level: BBa_K1321105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: Component
persistent identity: component2415334
display id: component2415334
version: 1
top level: BBa_K1321105
access: public
definition: BBa_K1321005

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415332
display id: annotation2415332
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415332/range2415332/1
component: BBa_B0105

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type: Range
persistent identity: range2415332
display id: range2415332
version: 1
direction: direction/reverse
top level: BBa_K1321105
start: 386
end: 391
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415327
display id: annotation2415327
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415327/range2415327/1
component: BBa_K1321338

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type: Range
persistent identity: range2415327
display id: range2415327
version: 1
direction: direction/reverse
top level: BBa_K1321105
start: 1
end: 43
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415329
display id: annotation2415329
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415329/range2415329/1
component: BBa_K1319106

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type: Range
persistent identity: range2415329
display id: range2415329
version: 1
direction: direction/reverse
top level: BBa_K1321105
start: 44
end: 58
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415330
display id: annotation2415330
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415330/range2415330/1
component: BBa_K1321003

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type: Range
persistent identity: range2415330
display id: range2415330
version: 1
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start: 59
end: 385
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2415334
display id: annotation2415334
version: 1
top level: BBa_K1321105
location: https://synbiohub.org/public/igem/BBa_K1321105/annotation2415334/range2415334/1
component: BBa_K1321005

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type: Range
persistent identity: range2415334
display id: range2415334
version: 1
direction: direction/reverse
top level: BBa_K1321105
start: 392
end: 514
orientation: inline

TerDnaE3(C
type: ComponentDefinition
persistent identity: BBa K1362131
display id: BBa_K1362131
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + TerDnaE3 C-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362405
component: BBa_K1362465
component: BBa_K1362418
component: BBa_K1362419
component: BBa_K1362421
component: BBa_K1362431
component: BBa_K1362422
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362414
component: BBa_K1362467
sequence annotation: BBa_K1362405
sequence annotation: BBa_K1362431
sequence annotation: BBa_K1362465
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362418
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362467
sequence annotation: BBa_K1362419
sequence annotation: BBa_G0000
sequence: https://synbiohub.org/public/igem/BBa_K1362131_sequence/1

BBa_K1362405
type: Component
persistent identity: component2410517
display id: component2410517
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362405/1

BBa_K1362465
type: Component
persistent identity: component2410542
display id: component2410542
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362465/1

BBa_K1362418
type: Component
persistent identity: component2410515
display id: component2410515
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362418/1

BBa_K1362419
type: Component
persistent identity: component2410518
display id: component2410518
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362421
type: Component
persistent identity: component2410541
display id: component2410541
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362431
type: Component
persistent identity: component2410514
display id: component2410514
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362431/1

BBa_K1362422
type: Component
persistent identity: component2410539
display id: component2410539
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362422/1

BBa_G0000
type: Component
persistent identity: component2410510
display id: component2410510
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2410509
display id: component2410509
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362414
type: Component
persistent identity: component2410511
display id: component2410511
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362467
type: Component
persistent identity: component2410516
display id: component2410516
version: 1
top level: TerDnaE3(C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362467/1

BBa_K1362405
type: SequenceAnnotation
persistent identity: annotation2410517
display id: annotation2410517
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410517/range2410517/1
component: BBa_K1362405

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type: Range
persistent identity: range2410517
display id: range2410517
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 66
end: 79
orientation: inline

BBa_K1362431
type: SequenceAnnotation
persistent identity: annotation2410514
display id: annotation2410514
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410514/range2410514/1
component: BBa_K1362431

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type: Range
persistent identity: range2410514
display id: range2410514
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 39
end: 59
orientation: inline

BBa_K1362465
type: SequenceAnnotation
persistent identity: annotation2410542
display id: annotation2410542
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410542/range2410542/1
component: BBa_K1362465

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410542/range2410542/1
type: Range
persistent identity: range2410542
display id: range2410542
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 1171
end: 1172
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410511
display id: annotation2410511
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410511/range2410511/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410511/range2410511/1
type: Range
persistent identity: range2410511
display id: range2410511
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 36
end: 38
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410509
display id: annotation2410509
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410509/range2410509/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410509/range2410509/1
type: Range
persistent identity: range2410509
display id: range2410509
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 1
end: 29
orientation: inline

BBa_K1362418
type: SequenceAnnotation
persistent identity: annotation2410515
display id: annotation2410515
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410515/range2410515/1
component: BBa_K1362418

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410515/range2410515/1
type: Range
persistent identity: range2410515
display id: range2410515
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 60
end: 63
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2410541
display id: annotation2410541
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410541/range2410541/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410541/range2410541/1
type: Range
persistent identity: range2410541
display id: range2410541
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 1167
end: 1170
orientation: inline

BBa_K1362422
type: SequenceAnnotation
persistent identity: annotation2410539
display id: annotation2410539
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410539/range2410539/1
component: BBa_K1362422

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410539/range2410539/1
type: Range
persistent identity: range2410539
display id: range2410539
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 84
end: 1166
orientation: inline

BBa_K1362467
type: SequenceAnnotation
persistent identity: annotation2410516
display id: annotation2410516
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410516/range2410516/1
component: BBa_K1362467

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410516/range2410516/1
type: Range
persistent identity: range2410516
display id: range2410516
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 64
end: 65
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2410518
display id: annotation2410518
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410518/range2410518/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362131/annotation2410518/range2410518/1
type: Range
persistent identity: range2410518
display id: range2410518
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 80
end: 83
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2410510
display id: annotation2410510
version: 1
top level: TerDnaE3(C
location: https://synbiohub.org/public/igem/BBa_K1362131/annotation2410510/range2410510/1
component: BBa_G0000

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type: Range
persistent identity: range2410510
display id: range2410510
version: 1
direction: direction/reverse
top level: TerDnaE3(C
start: 30
end: 35
orientation: inline

BBa_K1325007
type: ComponentDefinition
persistent identity: BBa K1325007
display id: BBa_K1325007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MinE
creator: Ke Xu
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
group u list: _1700_
m user id: 0
owner id: 23253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: cell division topological specificity factor MinE
mutable notes: no
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1325007_sequence/1

BBa_K1315005
type: ComponentDefinition
persistent identity: BBa K1315005
display id: BBa_K1315005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The clp gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: c-di-GMP Repressed DNA Binding Protein (Clp) Quorum sensing and bacterial cell signalling are mechanisms known to be utilised by bacteria to regulate their virulence. The RpfC-RpfG sensory system has been found to activate the production of virulence factors like extracellular enzymes and polysaccharides in Xanthomonas campestris. It has been also suggested that this system is involved in linking environmental stimuli, including DSF, with pathogenicity in Xanthomonas campestris. DSF is a cis-unsaturated fatty acid from the DSF family. The RpfC-RpfG two-component system is the most well understood one and is present in all Xanthomonads. http://www.ncbi.nlm.nih.gov/pubmed/?term=r+ryan+dsf+family http://www.ncbi.nlm.nih.gov/pubmed/?term=A+two-component+system+involving+an+HD-GYP+domain+protein+links+cell%E2%80%93cell+signalling+to+pathogenicity+gene+expression+in+Xanthomonas+campestris
mutable notes: The sequence had 1 PstI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Clp
sequence: https://synbiohub.org/public/igem/BBa_K1315005_sequence/1

Clp
type: SequenceAnnotation
persistent identity: annotation2386527
display id: annotation2386527
version: 1
top level: BBa_K1315005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1

https://synbiohub.org/public/igem/BBa_K1315005/annotation2386527/range2386527/1
type: Range
persistent identity: range2386527
display id: range2386527
version: 1
direction: direction/forward
top level: BBa_K1315005
start: 1
end: 696
orientation: inline

BBa_K1315009
type: ComponentDefinition
persistent identity: BBa K1315009
display id: BBa_K1315009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pseudomonas AutoInducer 1 (PAI-1) Biosensor
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a composite part that consists of 4 biobricks. In the front it has ptet(Bba_R0040) which is constitutively on. This produces LasR all the time with lasR(Bba_C0179) next in the sequence. Only in the presence of the Pseudomonas Autoinducer 1 (PA1), LasR forms an complex with PA1 and it can then activate the plasB(Bba_R0079) promoter that leads to the production of GFP(Bba_E0040).
mutable notes: All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.
mutable provenance: All the parts have come from existing iGEM distribution kits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_R0079
component: BBa_C0179
component: BBa_R0040
sequence annotation: BBa_E0040
sequence annotation: BBa_R0079
sequence annotation: BBa_C0179
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1315009_sequence/1

BBa_E0040
type: Component
persistent identity: component2387062
display id: component2387062
version: 1
top level: BBa_K1315009
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0079
type: Component
persistent identity: component2387060
display id: component2387060
version: 1
top level: BBa_K1315009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_C0179
type: Component
persistent identity: component2387055
display id: component2387055
version: 1
top level: BBa_K1315009
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_R0040
type: Component
persistent identity: component2387050
display id: component2387050
version: 1
top level: BBa_K1315009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2387062
display id: annotation2387062
version: 1
top level: BBa_K1315009
location: https://synbiohub.org/public/igem/BBa_K1315009/annotation2387062/range2387062/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1315009/annotation2387062/range2387062/1
type: Range
persistent identity: range2387062
display id: range2387062
version: 1
direction: direction/reverse
top level: BBa_K1315009
start: 955
end: 1674
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2387060
display id: annotation2387060
version: 1
top level: BBa_K1315009
location: https://synbiohub.org/public/igem/BBa_K1315009/annotation2387060/range2387060/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K1315009/annotation2387060/range2387060/1
type: Range
persistent identity: range2387060
display id: range2387060
version: 1
direction: direction/reverse
top level: BBa_K1315009
start: 792
end: 948
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2387055
display id: annotation2387055
version: 1
top level: BBa_K1315009
location: https://synbiohub.org/public/igem/BBa_K1315009/annotation2387055/range2387055/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K1315009/annotation2387055/range2387055/1
type: Range
persistent identity: range2387055
display id: range2387055
version: 1
direction: direction/reverse
top level: BBa_K1315009
start: 61
end: 783
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2387050
display id: annotation2387050
version: 1
top level: BBa_K1315009
location: https://synbiohub.org/public/igem/BBa_K1315009/annotation2387050/range2387050/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1315009/annotation2387050/range2387050/1
type: Range
persistent identity: range2387050
display id: range2387050
version: 1
direction: direction/forward
top level: BBa_K1315009
start: 1
end: 54
orientation: inline

BBa_K1315001
type: ComponentDefinition
persistent identity: BBa K1315001
display id: BBa_K1315001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The pqsA promoter from Pseudomonas
creator: Dimitrios Michailidis
created: 2014-09-19T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Pseudomonas quinolone signal (PQS) is imperative in modulating mechanisms that enable Pseudomonas aeruginosa to survive at times of stress. P. aeruginosa is the main pathogen responsible for severe deterioration in the Cystic Fibrosis lungs. The production of this signal relies on the pqsABCDE operon. The transcriptional factor PqsR (BBa_K1315000) positively regulates this operon. http://www.ncbi.nlm.nih.gov/pubmed/25225275 http://www.ncbi.nlm.nih.gov/pubmed/16735731 http://www.ncbi.nlm.nih.gov/pubmed/21539583 http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs http://www.ncbi.nlm.nih.gov/pubmed/12393198
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.
mutable provenance: The source of this part is the genome of Pseudomonas aeruginosa PAO1 provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pqsABCDE
sequence: https://synbiohub.org/public/igem/BBa_K1315001_sequence/1

pqsABCDE
type: SequenceAnnotation
persistent identity: annotation2384758
display id: annotation2384758
version: 1
top level: BBa_K1315001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1315001/annotation2384758/range2384758/1

https://synbiohub.org/public/igem/BBa_K1315001/annotation2384758/range2384758/1
type: Range
persistent identity: range2384758
display id: range2384758
version: 1
direction: direction/forward
top level: BBa_K1315001
start: 1
end: 92
orientation: inline

BBa_K1351021
type: ComponentDefinition
persistent identity: BBa K1351021
display id: BBa_K1351021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Monomeric Red Fluorescent Protein from Discosoma striata
creator: Anna Sommer
created: 2014-09-29T11:00:00Z
modified: 2016-01-26T01:58:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 4206
owner id: 20043
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant of the Biobrick BBa_E1010, in order to make it compatible with the Freiburg-Standard, two AgeI-Restriction sites were deleted monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
mutable notes: The Biobrick has the Following Prefix and Suffix: Gatcgaattccgcggccgcttctagataaggaggagccggc Gatcctgcagcggccgctactagtattaaccggt
mutable provenance: Discosoma striata (coral)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351021_sequence/1

BBa_K1491016
type: ComponentDefinition
persistent identity: BBa K1491016
display id: BBa_K1491016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Inducible Codon Optimized KillerRed
creator: Danielle Peters
created: 2014-08-03T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Codon optimized KillerRed with WTLac promoter allows for induction of KillerRed expression with IPTG.
mutable notes: Lac inducible codon optimized KillerRed
mutable provenance: The original KillerRed is BBa_K1184000.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1491015
component: BBa_J04500
sequence annotation: BBa_K1491015
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1491016_sequence/1

BBa_K1491015
type: Component
persistent identity: component2380355
display id: component2380355
version: 1
top level: BBa_K1491016
access: public
definition: BBa_K1491015

BBa_J04500
type: Component
persistent identity: component2380353
display id: component2380353
version: 1
top level: BBa_K1491016
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1491015
type: SequenceAnnotation
persistent identity: annotation2380355
display id: annotation2380355
version: 1
top level: BBa_K1491016
location: https://synbiohub.org/public/igem/BBa_K1491016/annotation2380355/range2380355/1
component: BBa_K1491015

https://synbiohub.org/public/igem/BBa_K1491016/annotation2380355/range2380355/1
type: Range
persistent identity: range2380355
display id: range2380355
version: 1
direction: direction/reverse
top level: BBa_K1491016
start: 227
end: 967
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2380353
display id: annotation2380353
version: 1
top level: BBa_K1491016
location: https://synbiohub.org/public/igem/BBa_K1491016/annotation2380353/range2380353/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1491016/annotation2380353/range2380353/1
type: Range
persistent identity: range2380353
display id: range2380353
version: 1
direction: direction/reverse
top level: BBa_K1491016
start: 1
end: 220
orientation: inline

BBa_K1321342
type: ComponentDefinition
persistent identity: BBa K1321342
display id: BBa_K1321342
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDcex in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:26:42Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: sadf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321337
component: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321337
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321342_sequence/1

BBa_B0105
type: Component
persistent identity: component2415361
display id: component2415361
version: 1
top level: BBa_K1321342
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321337
type: Component
persistent identity: component2415359
display id: component2415359
version: 1
top level: BBa_K1321342
access: public
definition: BBa_K1321337

BBa_K1321003
type: Component
persistent identity: component2415362
display id: component2415362
version: 1
top level: BBa_K1321342
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415361
display id: annotation2415361
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415361/range2415361/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415361/range2415361/1
type: Range
persistent identity: range2415361
display id: range2415361
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 712
end: 717
orientation: inline

BBa_K1321337
type: SequenceAnnotation
persistent identity: annotation2415359
display id: annotation2415359
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415359/range2415359/1
component: BBa_K1321337

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415359/range2415359/1
type: Range
persistent identity: range2415359
display id: range2415359
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 1
end: 711
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415362
display id: annotation2415362
version: 1
top level: BBa_K1321342
location: https://synbiohub.org/public/igem/BBa_K1321342/annotation2415362/range2415362/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321342/annotation2415362/range2415362/1
type: Range
persistent identity: range2415362
display id: range2415362
version: 1
direction: direction/reverse
top level: BBa_K1321342
start: 718
end: 1044
orientation: inline

BBa_K1321094
type: ComponentDefinition
persistent identity: BBa K1321094
display id: BBa_K1321094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to Phytochelatin (PC) EC20
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-06-17T12:17:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD4+PhytoC. TBC
mutable notes: CBD4+PhytoC. TBC
mutable provenance: CBD4+PhytoC. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321005
component: BBa_K1321002
component: BBa_B0105
sequence annotation: BBa_K1321005
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321094_sequence/1

BBa_K1321005
type: Component
persistent identity: component2414635
display id: component2414635
version: 1
top level: BBa_K1321094
access: public
definition: BBa_K1321005

BBa_K1321002
type: Component
persistent identity: component2414631
display id: component2414631
version: 1
top level: BBa_K1321094
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_B0105
type: Component
persistent identity: component2414633
display id: component2414633
version: 1
top level: BBa_K1321094
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414635
display id: annotation2414635
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414635/range2414635/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414635/range2414635/1
type: Range
persistent identity: range2414635
display id: range2414635
version: 1
direction: direction/reverse
top level: BBa_K1321094
start: 307
end: 429
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414633
display id: annotation2414633
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414633/range2414633/1
component: BBa_B0105

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type: Range
persistent identity: range2414633
display id: range2414633
version: 1
direction: direction/reverse
top level: BBa_K1321094
start: 301
end: 306
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414631
display id: annotation2414631
version: 1
top level: BBa_K1321094
location: https://synbiohub.org/public/igem/BBa_K1321094/annotation2414631/range2414631/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321094/annotation2414631/range2414631/1
type: Range
persistent identity: range2414631
display id: range2414631
version: 1
direction: direction/reverse
top level: BBa_K1321094
start: 1
end: 300
orientation: inline

BBa_K1351006
type: ComponentDefinition
persistent identity: BBa K1351006
display id: BBa_K1351006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CWB domain of B. subtilis major autolysin LytC
creator: Mona Dotzler
created: 2014-10-03T11:00:00Z
modified: 2015-06-01T12:54:45Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cell wall binding domain and signal peptide (amino acids 1 to 333) of ''Bacillus subtilis'' major autolysin LytC (Uniprot [http://www.uniprot.org/uniprot/Q02114 Q02114]). This domain has been used for displaying heterologous proteins on the cell surface of ''B. subtilis'' (Tsuchiya ''et. al.'', 1999). Used in the [http://2014.igem.org/Team:LMU-Munich/Project/Bakillus BaKillus project] to display pathogen-specific peptides by C-terminal fusions to mediate adhesion of ''B. subtlis'' to pathogens.
mutable notes: Due to the N-terminal signal peptide, this part allows only C-terminal fusions of proteins to be displayed on the cell surface. The CWB domain of LytC consists of three N‐terminal type II cell wall‐binding motifs, ranging from position 30 to 318. To minimize possible effects of a translational fusion to the CWB domain, the part includes the downstream unstructered region until position 333. An additional linker can be inserted to allow more structural flexibilty.
mutable provenance: This part was generated by amplification from ''B. subtilis'' W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3. LytC_ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC TTGCGTTCTTATATAAAAGTCCTAACAATGTG LytC_333_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT TGAATCTTGATCACCGTGACCC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CWB repeat
sequence annotation: signal peptide
sequence: https://synbiohub.org/public/igem/BBa_K1351006_sequence/1

CWB repeat
type: SequenceAnnotation
persistent identity: annotation2393769
display id: annotation2393769
version: 1
top level: BBa_K1351006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1351006/annotation2393769/range2393769/1

https://synbiohub.org/public/igem/BBa_K1351006/annotation2393769/range2393769/1
type: Range
persistent identity: range2393769
display id: range2393769
version: 1
direction: direction/forward
top level: BBa_K1351006
start: 669
end: 956
orientation: inline

CWB repeat
type: SequenceAnnotation
persistent identity: annotation2393767
display id: annotation2393767
version: 1
top level: BBa_K1351006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1351006/annotation2393767/range2393767/1

https://synbiohub.org/public/igem/BBa_K1351006/annotation2393767/range2393767/1
type: Range
persistent identity: range2393767
display id: range2393767
version: 1
direction: direction/forward
top level: BBa_K1351006
start: 90
end: 386
orientation: inline

signal peptide
type: SequenceAnnotation
persistent identity: annotation2393766
display id: annotation2393766
version: 1
top level: BBa_K1351006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1351006/annotation2393766/range2393766/1

https://synbiohub.org/public/igem/BBa_K1351006/annotation2393766/range2393766/1
type: Range
persistent identity: range2393766
display id: range2393766
version: 1
direction: direction/forward
top level: BBa_K1351006
start: 1
end: 75
orientation: inline

CWB repeat
type: SequenceAnnotation
persistent identity: annotation2393768
display id: annotation2393768
version: 1
top level: BBa_K1351006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1351006/annotation2393768/range2393768/1

https://synbiohub.org/public/igem/BBa_K1351006/annotation2393768/range2393768/1
type: Range
persistent identity: range2393768
display id: range2393768
version: 1
direction: direction/forward
top level: BBa_K1351006
start: 387
end: 668
orientation: inline

sfGFP_T65C
type: ComponentDefinition
persistent identity: BBa K1362170
display id: BBa_K1362170
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split sfGFP reconstitution positive control
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1362090
component: BBa_J70594
component: BBa_K371056
component: BBa_K1362468
component: BBa_G0000
component: BBa_K1362470
sequence annotation: BBa_J70594
sequence annotation: BBa_K1362468
sequence annotation: BBa_K371056
sequence annotation: BBa_K1362090
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362470
sequence: https://synbiohub.org/public/igem/BBa_K1362170_sequence/1

BBa_K1362090
type: Component
persistent identity: component2414456
display id: component2414456
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_J70594
type: Component
persistent identity: component2414464
display id: component2414464
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K371056
type: Component
persistent identity: component2414459
display id: component2414459
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_K371056/1

BBa_K1362468
type: Component
persistent identity: component2414462
display id: component2414462
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362468/1

BBa_G0000
type: Component
persistent identity: component2414457
display id: component2414457
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362468
type: Component
persistent identity: component2414460
display id: component2414460
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362468/1

BBa_K1362470
type: Component
persistent identity: component2414461
display id: component2414461
version: 1
top level: sfGFP_T65C
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362470/1

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2414464
display id: annotation2414464
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414464/range2414464/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362170/annotation2414464/range2414464/1
type: Range
persistent identity: range2414464
display id: range2414464
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 786
end: 791
orientation: inline

BBa_K1362468
type: SequenceAnnotation
persistent identity: annotation2414462
display id: annotation2414462
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414462/range2414462/1
component: BBa_K1362468

https://synbiohub.org/public/igem/BBa_K1362170/annotation2414462/range2414462/1
type: Range
persistent identity: range2414462
display id: range2414462
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 768
end: 785
orientation: inline

BBa_K371056
type: SequenceAnnotation
persistent identity: annotation2414459
display id: annotation2414459
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414459/range2414459/1
component: BBa_K371056

https://synbiohub.org/public/igem/BBa_K1362170/annotation2414459/range2414459/1
type: Range
persistent identity: range2414459
display id: range2414459
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 36
end: 38
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2414456
display id: annotation2414456
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414456/range2414456/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362170/annotation2414456/range2414456/1
type: Range
persistent identity: range2414456
display id: range2414456
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 1
end: 29
orientation: inline

BBa_K1362468
type: SequenceAnnotation
persistent identity: annotation2414460
display id: annotation2414460
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414460/range2414460/1
component: BBa_K1362468

https://synbiohub.org/public/igem/BBa_K1362170/annotation2414460/range2414460/1
type: Range
persistent identity: range2414460
display id: range2414460
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 39
end: 56
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2414457
display id: annotation2414457
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414457/range2414457/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362170/annotation2414457/range2414457/1
type: Range
persistent identity: range2414457
display id: range2414457
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 30
end: 35
orientation: inline

BBa_K1362470
type: SequenceAnnotation
persistent identity: annotation2414461
display id: annotation2414461
version: 1
top level: sfGFP_T65C
location: https://synbiohub.org/public/igem/BBa_K1362170/annotation2414461/range2414461/1
component: BBa_K1362470

https://synbiohub.org/public/igem/BBa_K1362170/annotation2414461/range2414461/1
type: Range
persistent identity: range2414461
display id: range2414461
version: 1
direction: direction/reverse
top level: sfGFP_T65C
start: 57
end: 767
orientation: inline

BBa_K1493501
type: ComponentDefinition
persistent identity: BBa K1493501
display id: BBa_K1493501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRha + GFP
creator: Michiel Herpers
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI repressor protein regulated by L-rhamnose promoter
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13504
component: BBa_K914003
sequence annotation: BBa_I13504
sequence annotation: BBa_K914003
sequence: https://synbiohub.org/public/igem/BBa_K1493501_sequence/1

BBa_I13504
type: Component
persistent identity: component2418209
display id: component2418209
version: 1
top level: BBa_K1493501
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_K914003
type: Component
persistent identity: component2418199
display id: component2418199
version: 1
top level: BBa_K1493501
access: public
definition: https://synbiohub.org/public/igem/BBa_K914003/1

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2418209
display id: annotation2418209
version: 1
top level: BBa_K1493501
location: https://synbiohub.org/public/igem/BBa_K1493501/annotation2418209/range2418209/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1493501/annotation2418209/range2418209/1
type: Range
persistent identity: range2418209
display id: range2418209
version: 1
direction: direction/reverse
top level: BBa_K1493501
start: 131
end: 1005
orientation: inline

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2418199
display id: annotation2418199
version: 1
top level: BBa_K1493501
location: https://synbiohub.org/public/igem/BBa_K1493501/annotation2418199/range2418199/1
component: BBa_K914003

https://synbiohub.org/public/igem/BBa_K1493501/annotation2418199/range2418199/1
type: Range
persistent identity: range2418199
display id: range2418199
version: 1
direction: direction/reverse
top level: BBa_K1493501
start: 1
end: 122
orientation: inline

BBa_K1323011
type: ComponentDefinition
persistent identity: BBa K1323011
display id: BBa_K1323011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Erythromycin resistance coding sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-28T01:42:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ermM CDS (Erythromycin resistance)
sequence: https://synbiohub.org/public/igem/BBa_K1323011_sequence/1

ermM CDS (Erythromycin resistance)
type: SequenceAnnotation
persistent identity: annotation2428789
display id: annotation2428789
version: 1
top level: BBa_K1323011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323011/annotation2428789/range2428789/1

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type: Range
persistent identity: range2428789
display id: range2428789
version: 1
direction: direction/forward
top level: BBa_K1323011
start: 1
end: 735
orientation: inline

BBa_K1362052
type: ComponentDefinition
persistent identity: BBa K1362052
display id: BBa_K1362052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Carrier for RFC[105] C-terminal inserts
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22911
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
component: BBa_K1362426
component: BBa_K1362421
component: BBa_K1362425
component: BBa_K1362436
component: BBa_K1362419
component: BBa_K1362423
sequence annotation: BBa_K1362426
sequence annotation: BBa_K1362425
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362436
sequence annotation: BBa_K1362419
sequence: https://synbiohub.org/public/igem/BBa_K1362052_sequence/1

BBa_K1362426
type: Component
persistent identity: component2409682
display id: component2409682
version: 1
top level: BBa_K1362052
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362426/1

BBa_K1362421
type: Component
persistent identity: component2409680
display id: component2409680
version: 1
top level: BBa_K1362052
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362425
type: Component
persistent identity: component2409676
display id: component2409676
version: 1
top level: BBa_K1362052
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362436
type: Component
persistent identity: component2409678
display id: component2409678
version: 1
top level: BBa_K1362052
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362436/1

BBa_K1362419
type: Component
persistent identity: component2409677
display id: component2409677
version: 1
top level: BBa_K1362052
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362423
type: Component
persistent identity: component2409681
display id: component2409681
version: 1
top level: BBa_K1362052
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362426
type: SequenceAnnotation
persistent identity: annotation2409682
display id: annotation2409682
version: 1
top level: BBa_K1362052
location: https://synbiohub.org/public/igem/BBa_K1362052/annotation2409682/range2409682/1
component: BBa_K1362426

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type: Range
persistent identity: range2409682
display id: range2409682
version: 1
direction: direction/reverse
top level: BBa_K1362052
start: 28
end: 31
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409676
display id: annotation2409676
version: 1
top level: BBa_K1362052
location: https://synbiohub.org/public/igem/BBa_K1362052/annotation2409676/range2409676/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362052/annotation2409676/range2409676/1
type: Range
persistent identity: range2409676
display id: range2409676
version: 1
direction: direction/reverse
top level: BBa_K1362052
start: 1
end: 6
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2409680
display id: annotation2409680
version: 1
top level: BBa_K1362052
location: https://synbiohub.org/public/igem/BBa_K1362052/annotation2409680/range2409680/1
component: BBa_K1362421

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type: Range
persistent identity: range2409680
display id: range2409680
version: 1
direction: direction/reverse
top level: BBa_K1362052
start: 17
end: 20
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409681
display id: annotation2409681
version: 1
top level: BBa_K1362052
location: https://synbiohub.org/public/igem/BBa_K1362052/annotation2409681/range2409681/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362052/annotation2409681/range2409681/1
type: Range
persistent identity: range2409681
display id: range2409681
version: 1
direction: direction/reverse
top level: BBa_K1362052
start: 21
end: 27
orientation: inline

BBa_K1362436
type: SequenceAnnotation
persistent identity: annotation2409678
display id: annotation2409678
version: 1
top level: BBa_K1362052
location: https://synbiohub.org/public/igem/BBa_K1362052/annotation2409678/range2409678/1
component: BBa_K1362436

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type: Range
persistent identity: range2409678
display id: range2409678
version: 1
direction: direction/reverse
top level: BBa_K1362052
start: 11
end: 16
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2409677
display id: annotation2409677
version: 1
top level: BBa_K1362052
location: https://synbiohub.org/public/igem/BBa_K1362052/annotation2409677/range2409677/1
component: BBa_K1362419

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type: Range
persistent identity: range2409677
display id: range2409677
version: 1
direction: direction/reverse
top level: BBa_K1362052
start: 7
end: 10
orientation: inline

BBa_K1404001
type: ComponentDefinition
persistent identity: BBa K1404001
display id: BBa_K1404001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptac-NiCoTB-rcn-csgBAEFG, turns E. coli into a functional Ni and Co biofilter
creator: Alexandre Duprey
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 8419
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part, when expressed in a rcnA E. coli strain, allows the formation of a functional Ni2+ and Co2+ biofilter capturing up to 3 mg of metal per g of bacterial dry weight when exposed to 20 ??M Ni2+ or Co2+, in only 10 mn. This is one of the best performances and the fastest reported until now.
mutable notes: Temp
mutable provenance: [lien] and [lien] were cloned by 3A assembly.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K540000
component: BBa_K1404002
sequence annotation: BBa_K540000
sequence annotation: BBa_K1404002
sequence: https://synbiohub.org/public/igem/BBa_K1404001_sequence/1

BBa_K540000
type: Component
persistent identity: component2385428
display id: component2385428
version: 1
top level: BBa_K1404001
access: public
definition: https://synbiohub.org/public/igem/BBa_K540000/1

BBa_K1404002
type: Component
persistent identity: component2385413
display id: component2385413
version: 1
top level: BBa_K1404001
access: public
definition: BBa_K1404002

BBa_K540000
type: SequenceAnnotation
persistent identity: annotation2385428
display id: annotation2385428
version: 1
top level: BBa_K1404001
location: https://synbiohub.org/public/igem/BBa_K1404001/annotation2385428/range2385428/1
component: BBa_K540000

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type: Range
persistent identity: range2385428
display id: range2385428
version: 1
direction: direction/reverse
top level: BBa_K1404001
start: 1315
end: 4437
orientation: inline

BBa_K1404002
type: SequenceAnnotation
persistent identity: annotation2385413
display id: annotation2385413
version: 1
top level: BBa_K1404001
location: https://synbiohub.org/public/igem/BBa_K1404001/annotation2385413/range2385413/1
component: BBa_K1404002

https://synbiohub.org/public/igem/BBa_K1404001/annotation2385413/range2385413/1
type: Range
persistent identity: range2385413
display id: range2385413
version: 1
direction: direction/reverse
top level: BBa_K1404001
start: 1
end: 1306
orientation: inline

BBa_K1497031
type: ComponentDefinition
persistent identity: BBa K1497031
display id: BBa_K1497031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold (G-S-P-His)
creator: Sascha Hein, Rene Sahm, Malte Blumenroth, Tobias Gabriel, Sebastian Palluk
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Scaffold (G-S-P-His)
mutable notes: Scaffold (G-S-P-His)
mutable provenance: Scaffold (G-S-P-His)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497031_sequence/1

BBa_K1321115
type: ComponentDefinition
persistent identity: BBa K1321115
display id: BBa_K1321115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDcex fused to phytochelatin (PC) EC20 with LacI promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:20:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD7+PhytoC with LacI
mutable notes: CBD7+PhytoC with LacI
mutable provenance: CBD7+PhytoC with LacI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321005
component: BBa_B0105
component: BBa_K1321003
component: BBa_J04500
sequence annotation: BBa_K1321005
sequence annotation: BBa_K1321003
sequence annotation: BBa_J04500
sequence annotation: BBa_K1319106
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321115_sequence/1

BBa_K1319106
type: Component
persistent identity: component2414980
display id: component2414980
version: 1
top level: BBa_K1321115
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321005
type: Component
persistent identity: component2414985
display id: component2414985
version: 1
top level: BBa_K1321115
access: public
definition: BBa_K1321005

BBa_B0105
type: Component
persistent identity: component2414983
display id: component2414983
version: 1
top level: BBa_K1321115
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321003
type: Component
persistent identity: component2414981
display id: component2414981
version: 1
top level: BBa_K1321115
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_J04500
type: Component
persistent identity: component2414978
display id: component2414978
version: 1
top level: BBa_K1321115
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2414985
display id: annotation2414985
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414985/range2414985/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414985/range2414985/1
type: Range
persistent identity: range2414985
display id: range2414985
version: 1
direction: direction/reverse
top level: BBa_K1321115
start: 569
end: 691
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414981
display id: annotation2414981
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414981/range2414981/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414981/range2414981/1
type: Range
persistent identity: range2414981
display id: range2414981
version: 1
direction: direction/reverse
top level: BBa_K1321115
start: 236
end: 562
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2414978
display id: annotation2414978
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414978/range2414978/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414978/range2414978/1
type: Range
persistent identity: range2414978
display id: range2414978
version: 1
direction: direction/reverse
top level: BBa_K1321115
start: 1
end: 220
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414980
display id: annotation2414980
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414980/range2414980/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414980/range2414980/1
type: Range
persistent identity: range2414980
display id: range2414980
version: 1
direction: direction/reverse
top level: BBa_K1321115
start: 221
end: 235
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414983
display id: annotation2414983
version: 1
top level: BBa_K1321115
location: https://synbiohub.org/public/igem/BBa_K1321115/annotation2414983/range2414983/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321115/annotation2414983/range2414983/1
type: Range
persistent identity: range2414983
display id: range2414983
version: 1
direction: direction/reverse
top level: BBa_K1321115
start: 563
end: 568
orientation: inline

Mj TyrRS
type: ComponentDefinition
persistent identity: BBa K1492002
display id: BBa_K1492002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Methanococcus jannaschii tyrosyl-tRNA synthetase
creator: Wiggert Altenburg
created: 2014-07-27T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1872_
m user id: 0
owner id: 21091
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Pyrrolysyl-tRNA Synthetase introduces the Non Natural Amino acid p-Azido-L-Phenylalanine at the TAG codon.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1492002_sequence/1

BBa_K1491005
type: ComponentDefinition
persistent identity: BBa K1491005
display id: BBa_K1491005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-SuperYFP2-His
creator: Danielle Peters
created: 2014-07-14T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: SuperYFP2 with 6x His tag(BBa_K1491004) and a strong RBS (BBa_B0034). It can be used as a reporter.
mutable notes: RBS+YFP+His
mutable provenance: RBS is BBa_B0034 and SuperYFP2 is BBa_K864100
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1491004
component: BBa_B0034
sequence annotation: BBa_K1491004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1491005_sequence/1

BBa_K1491004
type: Component
persistent identity: component2380100
display id: component2380100
version: 1
top level: BBa_K1491005
access: public
definition: https://synbiohub.org/public/igem/BBa_K1491004/1

BBa_B0034
type: Component
persistent identity: component2380097
display id: component2380097
version: 1
top level: BBa_K1491005
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1491004
type: SequenceAnnotation
persistent identity: annotation2380100
display id: annotation2380100
version: 1
top level: BBa_K1491005
location: https://synbiohub.org/public/igem/BBa_K1491005/annotation2380100/range2380100/1
component: BBa_K1491004

https://synbiohub.org/public/igem/BBa_K1491005/annotation2380100/range2380100/1
type: Range
persistent identity: range2380100
display id: range2380100
version: 1
direction: direction/reverse
top level: BBa_K1491005
start: 19
end: 759
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2380097
display id: annotation2380097
version: 1
top level: BBa_K1491005
location: https://synbiohub.org/public/igem/BBa_K1491005/annotation2380097/range2380097/1
component: BBa_B0034

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type: Range
persistent identity: range2380097
display id: range2380097
version: 1
direction: direction/reverse
top level: BBa_K1491005
start: 1
end: 12
orientation: inline

BBa_K1321338
type: ComponentDefinition
persistent identity: BBa K1321338
display id: BBa_K1321338
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Expression vector
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:53Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 0
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdfasdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_I719005
component: BBa_B0034
sequence annotation: BBa_I719005
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1321338_sequence/1

BBa_I719005
type: Component
persistent identity: component2402299
display id: component2402299
version: 1
top level: BBa_K1321338
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_B0034
type: Component
persistent identity: component2402301
display id: component2402301
version: 1
top level: BBa_K1321338
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2402299
display id: annotation2402299
version: 1
top level: BBa_K1321338
location: https://synbiohub.org/public/igem/BBa_K1321338/annotation2402299/range2402299/1
component: BBa_I719005

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type: Range
persistent identity: range2402299
display id: range2402299
version: 1
direction: direction/reverse
top level: BBa_K1321338
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2402301
display id: annotation2402301
version: 1
top level: BBa_K1321338
location: https://synbiohub.org/public/igem/BBa_K1321338/annotation2402301/range2402301/1
component: BBa_B0034

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type: Range
persistent identity: range2402301
display id: range2402301
version: 1
direction: direction/reverse
top level: BBa_K1321338
start: 32
end: 43
orientation: inline

BBa_K1362202
type: ComponentDefinition
persistent identity: BBa K1362202
display id: BBa_K1362202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Sortase A circularization construct (with His6; without RBS)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: TODO
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362454
component: BBa_K1362424
component: BBa_J70594
component: BBa_J04450
component: BBa_K1362427
component: BBa_K1362452
component: BBa_K1362445
component: BBa_K1362457
sequence annotation: BBa_K1362457
sequence annotation: BBa_J70594
sequence annotation: BBa_K1362427
sequence annotation: BBa_J04450
sequence annotation: BBa_K1362452
sequence annotation: BBa_K1362424
sequence annotation: BBa_K1362454
sequence annotation: BBa_K1362445
sequence: https://synbiohub.org/public/igem/BBa_K1362202_sequence/1

BBa_K1362454
type: Component
persistent identity: component2410786
display id: component2410786
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362454/1

BBa_K1362424
type: Component
persistent identity: component2410785
display id: component2410785
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362424/1

BBa_J70594
type: Component
persistent identity: component2410789
display id: component2410789
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_J04450
type: Component
persistent identity: component2410784
display id: component2410784
version: 1
top level: BBa_K1362202
access: public
definition: BBa_J04450

BBa_K1362427
type: Component
persistent identity: component2410766
display id: component2410766
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362427/1

BBa_K1362452
type: Component
persistent identity: component2410765
display id: component2410765
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362452/1

BBa_K1362445
type: Component
persistent identity: component2410764
display id: component2410764
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362445/1

BBa_K1362457
type: Component
persistent identity: component2410787
display id: component2410787
version: 1
top level: BBa_K1362202
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362457/1

BBa_K1362457
type: SequenceAnnotation
persistent identity: annotation2410787
display id: annotation2410787
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410787/range2410787/1
component: BBa_K1362457

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type: Range
persistent identity: range2410787
display id: range2410787
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 1134
end: 1151
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2410789
display id: annotation2410789
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410789/range2410789/1
component: BBa_J70594

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type: Range
persistent identity: range2410789
display id: range2410789
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 1152
end: 1157
orientation: inline

BBa_K1362427
type: SequenceAnnotation
persistent identity: annotation2410766
display id: annotation2410766
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410766/range2410766/1
component: BBa_K1362427

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type: Range
persistent identity: range2410766
display id: range2410766
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 34
end: 39
orientation: inline

BBa_J04450
type: SequenceAnnotation
persistent identity: annotation2410784
display id: annotation2410784
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410784/range2410784/1
component: BBa_J04450

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type: Range
persistent identity: range2410784
display id: range2410784
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 40
end: 1108
orientation: inline

BBa_K1362452
type: SequenceAnnotation
persistent identity: annotation2410765
display id: annotation2410765
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410765/range2410765/1
component: BBa_K1362452

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type: Range
persistent identity: range2410765
display id: range2410765
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 13
end: 33
orientation: inline

BBa_K1362424
type: SequenceAnnotation
persistent identity: annotation2410785
display id: annotation2410785
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410785/range2410785/1
component: BBa_K1362424

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type: Range
persistent identity: range2410785
display id: range2410785
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 1109
end: 1115
orientation: inline

BBa_K1362454
type: SequenceAnnotation
persistent identity: annotation2410786
display id: annotation2410786
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410786/range2410786/1
component: BBa_K1362454

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type: Range
persistent identity: range2410786
display id: range2410786
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 1116
end: 1133
orientation: inline

BBa_K1362445
type: SequenceAnnotation
persistent identity: annotation2410764
display id: annotation2410764
version: 1
top level: BBa_K1362202
location: https://synbiohub.org/public/igem/BBa_K1362202/annotation2410764/range2410764/1
component: BBa_K1362445

https://synbiohub.org/public/igem/BBa_K1362202/annotation2410764/range2410764/1
type: Range
persistent identity: range2410764
display id: range2410764
version: 1
direction: direction/reverse
top level: BBa_K1362202
start: 1
end: 12
orientation: inline

BBa_K1325002
type: ComponentDefinition
persistent identity: BBa K1325002
display id: BBa_K1325002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sicA*??????Chaperone
creator: Ke Xu
created: 2014-09-27T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
group u list: _1700_
m user id: 0
owner id: 23253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Chaperone,the SicA???InvF complex activates transcription from the psicA promoter
mutable notes: no
mutable provenance: type III secretion pathways
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1325002_sequence/1

BBa_K1499202
type: ComponentDefinition
persistent identity: BBa K1499202
display id: BBa_K1499202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Deinococcus radiodurans uracil-DNA glycosylase 2
creator: Raman Nelakanti
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1879_
m user id: 0
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: todo
mutable notes: todo
mutable provenance: todo
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA Stop
sequence annotation: Start Codon
sequence annotation: uracil-DNA glycosylase 2
sequence: https://synbiohub.org/public/igem/BBa_K1499202_sequence/1

TAA Stop
type: SequenceAnnotation
persistent identity: annotation2381353
display id: annotation2381353
version: 1
top level: BBa_K1499202
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1499202/annotation2381353/range2381353/1

https://synbiohub.org/public/igem/BBa_K1499202/annotation2381353/range2381353/1
type: Range
persistent identity: range2381353
display id: range2381353
version: 1
direction: direction/forward
top level: BBa_K1499202
start: 712
end: 714
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2381352
display id: annotation2381352
version: 1
top level: BBa_K1499202
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499202/annotation2381352/range2381352/1

https://synbiohub.org/public/igem/BBa_K1499202/annotation2381352/range2381352/1
type: Range
persistent identity: range2381352
display id: range2381352
version: 1
direction: direction/forward
top level: BBa_K1499202
start: 1
end: 3
orientation: inline

uracil-DNA glycosylase 2
type: SequenceAnnotation
persistent identity: annotation2429042
display id: annotation2429042
version: 1
top level: BBa_K1499202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1499202/annotation2429042/range2429042/1

https://synbiohub.org/public/igem/BBa_K1499202/annotation2429042/range2429042/1
type: Range
persistent identity: range2429042
display id: range2429042
version: 1
direction: direction/forward
top level: BBa_K1499202
start: 1
end: 714
orientation: inline

BBa_K1362172
type: ComponentDefinition
persistent identity: BBa K1362172
display id: BBa_K1362172
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Split sfGFP reconstitution C-terminal non-splicing control half
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 12377
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_G0000
component: BBa_K1362090
component: BBa_J18909
component: BBa_K1362479
component: BBa_K371056
component: BBa_K1362472
sequence annotation: BBa_K1362472
sequence annotation: BBa_J18909
sequence annotation: BBa_K1362090
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362479
sequence annotation: BBa_K371056
sequence: https://synbiohub.org/public/igem/BBa_K1362172_sequence/1

BBa_G0000
type: Component
persistent identity: component2414564
display id: component2414564
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2414563
display id: component2414563
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_J18909
type: Component
persistent identity: component2414568
display id: component2414568
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_J18909/1

BBa_K1362479
type: Component
persistent identity: component2414569
display id: component2414569
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362479/1

BBa_K371056
type: Component
persistent identity: component2414566
display id: component2414566
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_K371056/1

BBa_K1362472
type: Component
persistent identity: component2414573
display id: component2414573
version: 1
top level: BBa_K1362172
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362472/1

BBa_K1362472
type: SequenceAnnotation
persistent identity: annotation2414573
display id: annotation2414573
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414573/range2414573/1
component: BBa_K1362472

https://synbiohub.org/public/igem/BBa_K1362172/annotation2414573/range2414573/1
type: Range
persistent identity: range2414573
display id: range2414573
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 162
end: 707
orientation: inline

BBa_J18909
type: SequenceAnnotation
persistent identity: annotation2414568
display id: annotation2414568
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414568/range2414568/1
component: BBa_J18909

https://synbiohub.org/public/igem/BBa_K1362172/annotation2414568/range2414568/1
type: Range
persistent identity: range2414568
display id: range2414568
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 39
end: 56
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2414563
display id: annotation2414563
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414563/range2414563/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362172/annotation2414563/range2414563/1
type: Range
persistent identity: range2414563
display id: range2414563
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 1
end: 29
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2414564
display id: annotation2414564
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414564/range2414564/1
component: BBa_G0000

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type: Range
persistent identity: range2414564
display id: range2414564
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 30
end: 35
orientation: inline

BBa_K1362479
type: SequenceAnnotation
persistent identity: annotation2414569
display id: annotation2414569
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414569/range2414569/1
component: BBa_K1362479

https://synbiohub.org/public/igem/BBa_K1362172/annotation2414569/range2414569/1
type: Range
persistent identity: range2414569
display id: range2414569
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 57
end: 161
orientation: inline

BBa_K371056
type: SequenceAnnotation
persistent identity: annotation2414566
display id: annotation2414566
version: 1
top level: BBa_K1362172
location: https://synbiohub.org/public/igem/BBa_K1362172/annotation2414566/range2414566/1
component: BBa_K371056

https://synbiohub.org/public/igem/BBa_K1362172/annotation2414566/range2414566/1
type: Range
persistent identity: range2414566
display id: range2414566
version: 1
direction: direction/reverse
top level: BBa_K1362172
start: 36
end: 38
orientation: inline

BBa_K1351001
type: ComponentDefinition
persistent identity: BBa K1351001
display id: BBa_K1351001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Contactin-4-derived peptide binding to NADH Oxidase of S. pneumoniae
creator: Mona Dotzler
created: 2014-10-02T11:00:00Z
modified: 2015-06-01T12:51:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Short peptide of 13 amino acids corresponding to amino acids of the human protein Contactin-4 (isoform 3, UniProt Q8IWV2-3). It has been shown to bind to NADH oxidase (NOX) of Streptococcus pneumoniae and inhibit bacterial binding to human A549 cells (Muchnik et. al., 2013). Displayed on the surface of Bacillus subtilis, it should enable B. subtilis to bind specifically to S. pneumoniae. This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
mutable notes: For generation of the part's nucleotide sequence, the amino acid sequence of the peptide (Muchnik et. al., 2013) was reverse transcribed with regard to the codon usage of Bacillus subtilis. Oligos were designed as precut by EcoRI and PstI to shorten the synthesized fragments and to simplify the cloning into pSB1C3.
mutable provenance: The part was generated by annealing of artificially synthesized oligonucletides: C4P_ENX_SD_Ngo_precutEP_fwd: AATTCgcggccgctTCTAGAgtaaggaggaaGCCGGCATG TGGGAATGTAAAGCGAACGGAAGACCGAAACCGACATAT ACCGGTtaatACTAGTagcggccgCTGCA C4P_SNP_Age_precutEP_rev: GcggccgctACTAGTattaACCGGT ATATGTCGGTTTCGGTCTTCCGTTCGCTTTACATTCCCACATGCCGGCttcctccttacTCTAGAagcggccgcG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351001_sequence/1

BBa_K1321125
type: ComponentDefinition
persistent identity: BBa K1321125
display id: BBa_K1321125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBD7+Smt
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20961
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0105
component: BBa_K1321003
component: BBa_K1321011
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321003
sequence annotation: BBa_K1321011
sequence: https://synbiohub.org/public/igem/BBa_K1321125_sequence/1

BBa_B0105
type: Component
persistent identity: component2414714
display id: component2414714
version: 1
top level: BBa_K1321125
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321003
type: Component
persistent identity: component2414712
display id: component2414712
version: 1
top level: BBa_K1321125
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_K1321011
type: Component
persistent identity: component2414716
display id: component2414716
version: 1
top level: BBa_K1321125
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414714
display id: annotation2414714
version: 1
top level: BBa_K1321125
location: https://synbiohub.org/public/igem/BBa_K1321125/annotation2414714/range2414714/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321125/annotation2414714/range2414714/1
type: Range
persistent identity: range2414714
display id: range2414714
version: 1
direction: direction/reverse
top level: BBa_K1321125
start: 328
end: 333
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2414712
display id: annotation2414712
version: 1
top level: BBa_K1321125
location: https://synbiohub.org/public/igem/BBa_K1321125/annotation2414712/range2414712/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321125/annotation2414712/range2414712/1
type: Range
persistent identity: range2414712
display id: range2414712
version: 1
direction: direction/reverse
top level: BBa_K1321125
start: 1
end: 327
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414716
display id: annotation2414716
version: 1
top level: BBa_K1321125
location: https://synbiohub.org/public/igem/BBa_K1321125/annotation2414716/range2414716/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321125/annotation2414716/range2414716/1
type: Range
persistent identity: range2414716
display id: range2414716
version: 1
direction: direction/reverse
top level: BBa_K1321125
start: 334
end: 498
orientation: inline

BBa_K1323015
type: ComponentDefinition
persistent identity: BBa K1323015
display id: BBa_K1323015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: DsRed Expression Cassette under a Constitutive Promoter
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1698_
m user id: 0
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Terminator
sequence annotation: TIR RBS
sequence annotation: DsRed
sequence annotation: sarA P1 Promoter
sequence: https://synbiohub.org/public/igem/BBa_K1323015_sequence/1

Terminator
type: SequenceAnnotation
persistent identity: annotation2428769
display id: annotation2428769
version: 1
top level: BBa_K1323015
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1323015/annotation2428769/range2428769/1

https://synbiohub.org/public/igem/BBa_K1323015/annotation2428769/range2428769/1
type: Range
persistent identity: range2428769
display id: range2428769
version: 1
direction: direction/forward
top level: BBa_K1323015
start: 765
end: 866
orientation: inline

TIR RBS
type: SequenceAnnotation
persistent identity: annotation2428767
display id: annotation2428767
version: 1
top level: BBa_K1323015
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1323015/annotation2428767/range2428767/1

https://synbiohub.org/public/igem/BBa_K1323015/annotation2428767/range2428767/1
type: Range
persistent identity: range2428767
display id: range2428767
version: 1
direction: direction/forward
top level: BBa_K1323015
start: 45
end: 72
orientation: inline

DsRed
type: SequenceAnnotation
persistent identity: annotation2428768
display id: annotation2428768
version: 1
top level: BBa_K1323015
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323015/annotation2428768/range2428768/1

https://synbiohub.org/public/igem/BBa_K1323015/annotation2428768/range2428768/1
type: Range
persistent identity: range2428768
display id: range2428768
version: 1
direction: direction/forward
top level: BBa_K1323015
start: 79
end: 756
orientation: inline

sarA P1 Promoter
type: SequenceAnnotation
persistent identity: annotation2428766
display id: annotation2428766
version: 1
top level: BBa_K1323015
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1323015/annotation2428766/range2428766/1

https://synbiohub.org/public/igem/BBa_K1323015/annotation2428766/range2428766/1
type: Range
persistent identity: range2428766
display id: range2428766
version: 1
direction: direction/forward
top level: BBa_K1323015
start: 1
end: 36
orientation: inline

BBa_K1415006
type: ComponentDefinition
persistent identity: BBa K1415006
display id: BBa_K1415006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAN (Helicoverpa armigera Hubner)
creator: HO, TSUNG YU
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Cotton bollworm Helicoverpa armigera （Hubner） Spread:The pink bollworm has spread to cotton-growing regions throughout the world. Characteristics: The larva is green, khaki, grey-brown or brown with dark spots. The topside is darker than the bottom side and a yellow or light brown stripe goes round the middle portion by the spots. Damage: The cotton bollworm is a moth, the larvae of which feed on a wide range of plants, including many important cultivated crops. It is a major pest in cotton and one of the most polyphagous and cosmopolitan pest species. It should not be confused with the similarly named, related species Helicoverpa zea.The cotton bollworm is a highly polyphagous species.The most important crop hosts are tomato, cotton, pigeon pea, chickpea, sorghum and cowpea. Other hosts include groundnut, okra, peas, field beans, soybeans, lucerne, Phaseolus spp., other Leguminosae, tobacco, potatoes, maize, flax, Dianthus, Rosa, Pelargonium, Chrysanthemum, a number of fruit trees, forest trees and a range of vegetable crops. Control: Cultural controls, with the exception of the use of Bt cotton and the use of mating disruption and sprays of the Entrust formulation of spinosad are acceptable to use on organically grown cotton.
mutable notes: No
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PBAN (Helicoverpa armigera Hubner)
sequence: https://synbiohub.org/public/igem/BBa_K1415006_sequence/1

PBAN (Helicoverpa armigera Hubner)
type: SequenceAnnotation
persistent identity: annotation2392678
display id: annotation2392678
version: 1
top level: BBa_K1415006
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1415006/annotation2392678/range2392678/1

https://synbiohub.org/public/igem/BBa_K1415006/annotation2392678/range2392678/1
type: Range
persistent identity: range2392678
display id: range2392678
version: 1
direction: direction/forward
top level: BBa_K1415006
start: 1
end: 109
orientation: inline

BBa_K1415108
type: ComponentDefinition
persistent identity: BBa K1415108
display id: BBa_K1415108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons+B0034+PBAN(Solenopsis invicta)
creator: HO, TSUNG YU
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has a constitutive promoter, so our PBAN(Solenopsis invicta) can constitutive express.
mutable notes: No
mutable provenance: Assemble
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1415008
component: BBa_J23101
sequence annotation: BBa_J23101
sequence annotation: BBa_B0034
sequence annotation: BBa_K1415008
sequence: https://synbiohub.org/public/igem/BBa_K1415108_sequence/1

BBa_B0034
type: Component
persistent identity: component2393997
display id: component2393997
version: 1
top level: BBa_K1415108
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1415008
type: Component
persistent identity: component2393999
display id: component2393999
version: 1
top level: BBa_K1415108
access: public
definition: BBa_K1415008

BBa_J23101
type: Component
persistent identity: component2393995
display id: component2393995
version: 1
top level: BBa_K1415108
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2393995
display id: annotation2393995
version: 1
top level: BBa_K1415108
location: https://synbiohub.org/public/igem/BBa_K1415108/annotation2393995/range2393995/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1415108/annotation2393995/range2393995/1
type: Range
persistent identity: range2393995
display id: range2393995
version: 1
direction: direction/reverse
top level: BBa_K1415108
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393997
display id: annotation2393997
version: 1
top level: BBa_K1415108
location: https://synbiohub.org/public/igem/BBa_K1415108/annotation2393997/range2393997/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1415108/annotation2393997/range2393997/1
type: Range
persistent identity: range2393997
display id: range2393997
version: 1
direction: direction/reverse
top level: BBa_K1415108
start: 44
end: 55
orientation: inline

BBa_K1415008
type: SequenceAnnotation
persistent identity: annotation2393999
display id: annotation2393999
version: 1
top level: BBa_K1415108
location: https://synbiohub.org/public/igem/BBa_K1415108/annotation2393999/range2393999/1
component: BBa_K1415008

https://synbiohub.org/public/igem/BBa_K1415108/annotation2393999/range2393999/1
type: Range
persistent identity: range2393999
display id: range2393999
version: 1
direction: direction/reverse
top level: BBa_K1415108
start: 62
end: 149
orientation: inline

BBa_K1315011
type: ComponentDefinition
persistent identity: BBa K1315011
display id: BBa_K1315011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pseudomonas AutoInducer-1 response unit (lasB promoter)
creator: Dimitrios Michailidis
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ptet+lasR+plasB
mutable notes: All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.
mutable provenance: All the parts have come from existing iGEM distribution kits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0079
component: BBa_C0179
component: BBa_R0040
sequence annotation: BBa_C0179
sequence annotation: BBa_R0079
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_K1315011_sequence/1

BBa_R0079
type: Component
persistent identity: component2387455
display id: component2387455
version: 1
top level: BBa_K1315011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0079/1

BBa_C0179
type: Component
persistent identity: component2387450
display id: component2387450
version: 1
top level: BBa_K1315011
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_R0040
type: Component
persistent identity: component2387445
display id: component2387445
version: 1
top level: BBa_K1315011
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2387450
display id: annotation2387450
version: 1
top level: BBa_K1315011
location: https://synbiohub.org/public/igem/BBa_K1315011/annotation2387450/range2387450/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K1315011/annotation2387450/range2387450/1
type: Range
persistent identity: range2387450
display id: range2387450
version: 1
direction: direction/reverse
top level: BBa_K1315011
start: 61
end: 783
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2387455
display id: annotation2387455
version: 1
top level: BBa_K1315011
location: https://synbiohub.org/public/igem/BBa_K1315011/annotation2387455/range2387455/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K1315011/annotation2387455/range2387455/1
type: Range
persistent identity: range2387455
display id: range2387455
version: 1
direction: direction/reverse
top level: BBa_K1315011
start: 792
end: 948
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2387445
display id: annotation2387445
version: 1
top level: BBa_K1315011
location: https://synbiohub.org/public/igem/BBa_K1315011/annotation2387445/range2387445/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1315011/annotation2387445/range2387445/1
type: Range
persistent identity: range2387445
display id: range2387445
version: 1
direction: direction/forward
top level: BBa_K1315011
start: 1
end: 54
orientation: inline

BBa_K1321159
type: ComponentDefinition
persistent identity: BBa K1321159
display id: BBa_K1321159
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fmt metallothionein with T7 promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321338
component: BBa_K1321012
component: BBa_K1319106
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321012
sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321159_sequence/1

BBa_K1321338
type: Component
persistent identity: component2414965
display id: component2414965
version: 1
top level: BBa_K1321159
access: public
definition: BBa_K1321338

BBa_K1321012
type: Component
persistent identity: component2414969
display id: component2414969
version: 1
top level: BBa_K1321159
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321012/1

BBa_K1319106
type: Component
persistent identity: component2414967
display id: component2414967
version: 1
top level: BBa_K1321159
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414967
display id: annotation2414967
version: 1
top level: BBa_K1321159
location: https://synbiohub.org/public/igem/BBa_K1321159/annotation2414967/range2414967/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321159/annotation2414967/range2414967/1
type: Range
persistent identity: range2414967
display id: range2414967
version: 1
direction: direction/reverse
top level: BBa_K1321159
start: 44
end: 58
orientation: inline

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2414969
display id: annotation2414969
version: 1
top level: BBa_K1321159
location: https://synbiohub.org/public/igem/BBa_K1321159/annotation2414969/range2414969/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321159/annotation2414969/range2414969/1
type: Range
persistent identity: range2414969
display id: range2414969
version: 1
direction: direction/reverse
top level: BBa_K1321159
start: 59
end: 256
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2414965
display id: annotation2414965
version: 1
top level: BBa_K1321159
location: https://synbiohub.org/public/igem/BBa_K1321159/annotation2414965/range2414965/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321159/annotation2414965/range2414965/1
type: Range
persistent identity: range2414965
display id: range2414965
version: 1
direction: direction/reverse
top level: BBa_K1321159
start: 1
end: 43
orientation: inline

PfrI
type: ComponentDefinition
persistent identity: BBa K1493200
display id: BBa_K1493200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ferric regulator PfrI
creator: Wen Wu
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcription factor involved in pyoverdine synethesis gene transcription
mutable notes: -
mutable provenance: ?
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PfrI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K1493200_sequence/1

PfrI
type: SequenceAnnotation
persistent identity: annotation2401019
display id: annotation2401019
version: 1
top level: PfrI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1493200/annotation2401019/range2401019/1

https://synbiohub.org/public/igem/BBa_K1493200/annotation2401019/range2401019/1
type: Range
persistent identity: range2401019
display id: range2401019
version: 1
direction: direction/forward
top level: PfrI
start: 18
end: 568
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2401018
display id: annotation2401018
version: 1
top level: PfrI
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1493200/annotation2401018/range2401018/1

https://synbiohub.org/public/igem/BBa_K1493200/annotation2401018/range2401018/1
type: Range
persistent identity: range2401018
display id: range2401018
version: 1
direction: direction/forward
top level: PfrI
start: 1
end: 12
orientation: inline

BBa_K1393003
type: ComponentDefinition
persistent identity: BBa K1393003
display id: BBa_K1393003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: outer membrane porin protein C from Escherichia coli BL21(DE3)
creator: Jiajun Tan
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
group u list: _1770_
m user id: 0
owner id: 20256
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: One OmpC molecule consists of 16 transmembrane, antiparallel β-strands, which produce a β-barrel structure surrounding a large channel and are connected by seven internal loops and eight external loops. Three OmpC molecules form a pore structure on the outer membrane of an E.coli cell, which allows small hydrophilic molecules to pass through.
mutable notes: In general, the amino acid sequences of the external loops are less conservative, and thus these loops may be relatively tolerant to insertion and deletion. We used the external loop as the point of insertion for foreign peptides (copper binding polypeptide or polyhistidine) for cell surface display.
mutable provenance: This part is from genome of Escherichia coli BL21(DE3).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: A to G
sequence annotation: G to A
sequence: https://synbiohub.org/public/igem/BBa_K1393003_sequence/1

A to G
type: SequenceAnnotation
persistent identity: annotation2414293
display id: annotation2414293
version: 1
top level: BBa_K1393003
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1393003/annotation2414293/range2414293/1

https://synbiohub.org/public/igem/BBa_K1393003/annotation2414293/range2414293/1
type: Range
persistent identity: range2414293
display id: range2414293
version: 1
direction: direction/forward
top level: BBa_K1393003
start: 222
end: 223
orientation: inline

G to A
type: SequenceAnnotation
persistent identity: annotation2414296
display id: annotation2414296
version: 1
top level: BBa_K1393003
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1393003/annotation2414296/range2414296/1

https://synbiohub.org/public/igem/BBa_K1393003/annotation2414296/range2414296/1
type: Range
persistent identity: range2414296
display id: range2414296
version: 1
direction: direction/forward
top level: BBa_K1393003
start: 731
end: 732
orientation: inline

A to G
type: SequenceAnnotation
persistent identity: annotation2414295
display id: annotation2414295
version: 1
top level: BBa_K1393003
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K1393003/annotation2414295/range2414295/1

https://synbiohub.org/public/igem/BBa_K1393003/annotation2414295/range2414295/1
type: Range
persistent identity: range2414295
display id: range2414295
version: 1
direction: direction/forward
top level: BBa_K1393003
start: 378
end: 379
orientation: inline

BBa_K1362160
type: ComponentDefinition
persistent identity: BBa K1362160
display id: BBa_K1362160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)
creator: Jan Gleixner
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_G0000
component: BBa_K1362417
component: BBa_K1362437
component: BBa_K1362415
component: BBa_K1362090
component: BBa_K1362430
component: BBa_K1362414
component: BBa_K1362421
component: BBa_K1362412
component: BBa_K1362422
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362430
sequence annotation: BBa_K1362422
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362412
sequence annotation: BBa_K1362437
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362417
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362090
sequence: https://synbiohub.org/public/igem/BBa_K1362160_sequence/1

BBa_G0000
type: Component
persistent identity: component2413327
display id: component2413327
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362417
type: Component
persistent identity: component2413354
display id: component2413354
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362417/1

BBa_K1362437
type: Component
persistent identity: component2413361
display id: component2413361
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362437/1

BBa_K1362415
type: Component
persistent identity: component2413350
display id: component2413350
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362090
type: Component
persistent identity: component2413326
display id: component2413326
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362430
type: Component
persistent identity: component2413357
display id: component2413357
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362430/1

BBa_K1362414
type: Component
persistent identity: component2413328
display id: component2413328
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362421
type: Component
persistent identity: component2413359
display id: component2413359
version: 1
top level: BBa_K1362160
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362412
type: Component
persistent identity: component2413351
display id: component2413351
version: 1
top level: BBa_K1362160
access: public
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TerDnaE3(N
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display id: BBa_K1362130
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + TerDnaE3 N-intein RFC[105] assembly construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
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modified: 2015-05-08T01:10:04Z
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experience: experience/None
group u list: _1738_
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sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
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star: false
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SspDnaB(N*
type: ComponentDefinition
persistent identity: BBa K1362142
display id: BBa_K1362142
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 12377
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sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
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BBa_G0000
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type: Range
persistent identity: range2410646
display id: range2410646
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 1161
end: 1166
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2410620
display id: annotation2410620
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410620/range2410620/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410620/range2410620/1
type: Range
persistent identity: range2410620
display id: range2410620
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 36
end: 38
orientation: inline

BBa_K1362408
type: SequenceAnnotation
persistent identity: annotation2410643
display id: annotation2410643
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410643/range2410643/1
component: BBa_K1362408

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410643/range2410643/1
type: Range
persistent identity: range2410643
display id: range2410643
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 1126
end: 1160
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2410618
display id: annotation2410618
version: 1
top level: SspDnaB(N*
location: https://synbiohub.org/public/igem/BBa_K1362142/annotation2410618/range2410618/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362142/annotation2410618/range2410618/1
type: Range
persistent identity: range2410618
display id: range2410618
version: 1
direction: direction/reverse
top level: SspDnaB(N*
start: 1
end: 29
orientation: inline

BBa_K1404006
type: ComponentDefinition
persistent identity: BBa K1404006
display id: BBa_K1404006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA, curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: RBS Paris 2009
sequence annotation: Beta sheet5
sequence annotation: Beta sheet1
sequence annotation: CsgA-WT
sequence annotation: CsgG signal
sequence annotation: Beta sheet3
sequence annotation: mutation
sequence annotation: Beta sheet2
sequence annotation: Sec signal
sequence annotation: Beta sheet4
sequence annotation: Part:BBa_K342000
sequence: https://synbiohub.org/public/igem/BBa_K1404006_sequence/1

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2425875
display id: annotation2425875
version: 1
top level: BBa_K1404006
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425875/range2425875/1
type: Range
persistent identity: range2425875
display id: range2425875
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 79
end: 91
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2425863
display id: annotation2425863
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425863/range2425863/1
type: Range
persistent identity: range2425863
display id: range2425863
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 488
end: 544
orientation: inline

Beta sheet1
type: SequenceAnnotation
persistent identity: annotation2425859
display id: annotation2425859
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425859/range2425859/1
type: Range
persistent identity: range2425859
display id: range2425859
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 218
end: 286
orientation: inline

CsgA-WT
type: SequenceAnnotation
persistent identity: annotation2425824
display id: annotation2425824
version: 1
top level: BBa_K1404006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425824/range2425824/1
type: Range
persistent identity: range2425824
display id: range2425824
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 547
orientation: inline

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2425825
display id: annotation2425825
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425825/range2425825/1
type: Range
persistent identity: range2425825
display id: range2425825
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 152
end: 217
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2425861
display id: annotation2425861
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425861/range2425861/1
type: Range
persistent identity: range2425861
display id: range2425861
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 353
end: 421
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2425844
display id: annotation2425844
version: 1
top level: BBa_K1404006
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425844/range2425844/1
type: Range
persistent identity: range2425844
display id: range2425844
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 461
end: 463
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2425860
display id: annotation2425860
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425860/range2425860/1
type: Range
persistent identity: range2425860
display id: range2425860
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 287
end: 352
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2425823
display id: annotation2425823
version: 1
top level: BBa_K1404006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425823/range2425823/1
type: Range
persistent identity: range2425823
display id: range2425823
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 92
end: 151
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2425862
display id: annotation2425862
version: 1
top level: BBa_K1404006
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425862/range2425862/1
type: Range
persistent identity: range2425862
display id: range2425862
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 422
end: 487
orientation: inline

Part:BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2425822
display id: annotation2425822
version: 1
top level: BBa_K1404006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1

https://synbiohub.org/public/igem/BBa_K1404006/annotation2425822/range2425822/1
type: Range
persistent identity: range2425822
display id: range2425822
version: 1
direction: direction/forward
top level: BBa_K1404006
start: 1
end: 70
orientation: inline

BBa_K1333300
type: ComponentDefinition
persistent identity: BBa K1333300
display id: BBa_K1333300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13453-Rose42-RFP
creator: Runwen Yao
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15873
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a combination of a pBAD promoter(BBa_I13453), RNA thermometer(BBa_K115001), and a RFP gene.
mutable notes: nothing
mutable provenance: All this parts come from the distribution.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K115001
component: BBa_E1010
component: BBa_I13453
sequence annotation: BBa_E1010
sequence annotation: BBa_K115001
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1333300_sequence/1

BBa_K115001
type: Component
persistent identity: component2385900
display id: component2385900
version: 1
top level: BBa_K1333300
access: public
definition: https://synbiohub.org/public/igem/BBa_K115001/1

BBa_E1010
type: Component
persistent identity: component2385903
display id: component2385903
version: 1
top level: BBa_K1333300
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_I13453
type: Component
persistent identity: component2385893
display id: component2385893
version: 1
top level: BBa_K1333300
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2385903
display id: annotation2385903
version: 1
top level: BBa_K1333300
location: https://synbiohub.org/public/igem/BBa_K1333300/annotation2385903/range2385903/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1333300/annotation2385903/range2385903/1
type: Range
persistent identity: range2385903
display id: range2385903
version: 1
direction: direction/reverse
top level: BBa_K1333300
start: 241
end: 946
orientation: inline

BBa_K115001
type: SequenceAnnotation
persistent identity: annotation2385900
display id: annotation2385900
version: 1
top level: BBa_K1333300
location: https://synbiohub.org/public/igem/BBa_K1333300/annotation2385900/range2385900/1
component: BBa_K115001

https://synbiohub.org/public/igem/BBa_K1333300/annotation2385900/range2385900/1
type: Range
persistent identity: range2385900
display id: range2385900
version: 1
direction: direction/reverse
top level: BBa_K1333300
start: 139
end: 234
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2385893
display id: annotation2385893
version: 1
top level: BBa_K1333300
location: https://synbiohub.org/public/igem/BBa_K1333300/annotation2385893/range2385893/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1333300/annotation2385893/range2385893/1
type: Range
persistent identity: range2385893
display id: range2385893
version: 1
direction: direction/reverse
top level: BBa_K1333300
start: 1
end: 130
orientation: inline

BBa_K1499004
type: ComponentDefinition
persistent identity: BBa K1499004
display id: BBa_K1499004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cellulose binding domains with streptavidin domain generator
creator: Raman Nelakanti
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:53:51Z
discontinued: false
dominant: false
group u list: _1879_
m user id: 4206
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: todo
mutable notes: Different sequences for cellulose-binding domains to prevent homologous recombination. Removed cut sites for RFC-10 compatibility.
mutable provenance: organism 1 organism 2
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Cellulose-binding(clostridium cellulovorans)
sequence annotation: Streptavidin
sequence: https://synbiohub.org/public/igem/BBa_K1499004_sequence/1

Cellulose-binding(clostridium cellulovorans)
type: SequenceAnnotation
persistent identity: annotation2429345
display id: annotation2429345
version: 1
top level: BBa_K1499004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1499004/annotation2429345/range2429345/1

https://synbiohub.org/public/igem/BBa_K1499004/annotation2429345/range2429345/1
type: Range
persistent identity: range2429345
display id: range2429345
version: 1
direction: direction/forward
top level: BBa_K1499004
start: 55
end: 352
orientation: inline

Streptavidin
type: SequenceAnnotation
persistent identity: annotation2429346
display id: annotation2429346
version: 1
top level: BBa_K1499004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1499004/annotation2429346/range2429346/1

https://synbiohub.org/public/igem/BBa_K1499004/annotation2429346/range2429346/1
type: Range
persistent identity: range2429346
display id: range2429346
version: 1
direction: direction/forward
top level: BBa_K1499004
start: 373
end: 750
orientation: inline

BBa_K1351010
type: ComponentDefinition
persistent identity: BBa K1351010
display id: BBa_K1351010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cell wall-anchoring domain of B. subtilis endonuclease YhcR
creator: Mona Dotzler
created: 2014-10-04T11:00:00Z
modified: 2015-06-01T12:55:21Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cell wall-anchoring domain (amino acids 1086 to 1217) of ''Bacillus subtilis'' endonuclease YhcR (UniProt [http://www.uniprot.org/uniprot/P54602 P54602]). YhcR is secreted and covalently anchored to the petidoglycan wall by the sortase YhcS. A YhcR-based surface display system has been shown to covalently anchor heterologous proteins to the ''B. subtilis'' cell wall (Liew ''et. al.'', 2012). It was used in the [http://2014.igem.org/Team:LMU-Munich/Project/Bakillus BaKillus project] to display pathogen-specific peptides and mediate adhesion of ''B. subtlis'' to pathogens. It consists of four components: the sortase substrate with a signal peptide ([http://parts.igem.org/Part:BBa_K1351008 BBa_K1351008]), a linker ([http://parts.igem.org/Part:BBa_K1351009 BBa_K1351009]) and a cell wall-anchoring domain (this part) as well as the sortase YhcS itself ([http://parts.igem.org/Part:BBa_K1351011 BBa_K1351011]), whose overexpression increases the efficiency of the surface display. This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
mutable notes: none
mutable provenance: This part was generated by amplification from ''B. subtilis'' W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3. CWAD_YhcR_ ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGCATG GCTGAAGCCAGTGATATTGAAGACCC CWAD_YhcR_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT TCACGTTCTGGAGGCGCTC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LPDTS sorting signal
sequence: https://synbiohub.org/public/igem/BBa_K1351010_sequence/1

LPDTS sorting signal
type: SequenceAnnotation
persistent identity: annotation2394181
display id: annotation2394181
version: 1
top level: BBa_K1351010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1351010/annotation2394181/range2394181/1

https://synbiohub.org/public/igem/BBa_K1351010/annotation2394181/range2394181/1
type: Range
persistent identity: range2394181
display id: range2394181
version: 1
direction: direction/forward
top level: BBa_K1351010
start: 292
end: 306
orientation: inline

BBa_K1321162
type: ComponentDefinition
persistent identity: BBa K1321162
display id: BBa_K1321162
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP driven by LacI promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321009
component: BBa_J04500
component: BBa_K1319106
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321009
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1321162_sequence/1

BBa_K1321009
type: Component
persistent identity: component2415069
display id: component2415069
version: 1
top level: BBa_K1321162
access: public
definition: BBa_K1321009

BBa_J04500
type: Component
persistent identity: component2415066
display id: component2415066
version: 1
top level: BBa_K1321162
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1319106
type: Component
persistent identity: component2415068
display id: component2415068
version: 1
top level: BBa_K1321162
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415068
display id: annotation2415068
version: 1
top level: BBa_K1321162
location: https://synbiohub.org/public/igem/BBa_K1321162/annotation2415068/range2415068/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321162/annotation2415068/range2415068/1
type: Range
persistent identity: range2415068
display id: range2415068
version: 1
direction: direction/reverse
top level: BBa_K1321162
start: 221
end: 235
orientation: inline

BBa_K1321009
type: SequenceAnnotation
persistent identity: annotation2415069
display id: annotation2415069
version: 1
top level: BBa_K1321162
location: https://synbiohub.org/public/igem/BBa_K1321162/annotation2415069/range2415069/1
component: BBa_K1321009

https://synbiohub.org/public/igem/BBa_K1321162/annotation2415069/range2415069/1
type: Range
persistent identity: range2415069
display id: range2415069
version: 1
direction: direction/reverse
top level: BBa_K1321162
start: 236
end: 411
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415066
display id: annotation2415066
version: 1
top level: BBa_K1321162
location: https://synbiohub.org/public/igem/BBa_K1321162/annotation2415066/range2415066/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321162/annotation2415066/range2415066/1
type: Range
persistent identity: range2415066
display id: range2415066
version: 1
direction: direction/reverse
top level: BBa_K1321162
start: 1
end: 220
orientation: inline

BBa_K1375002
type: ComponentDefinition
persistent identity: BBa K1375002
display id: BBa_K1375002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PRhl/Las+RBS+GFP+DT
creator: Zheng Xu
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
group u list: _1752_
m user id: 0
owner id: 18474
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a reporter biobrick that can be regulated by C4-HSL and reduced by C12-HSL, also,there need another two coding sequences, RhlR and LasR.
mutable notes: NO
mutable provenance: BBa_K1375024 , I13401 and B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K1375024
component: BBa_I13401
sequence annotation: BBa_I13401
sequence annotation: BBa_K1375024
sequence: https://synbiohub.org/public/igem/BBa_K1375002_sequence/1

BBa_K1375024
type: Component
persistent identity: component2418710
display id: component2418710
version: 1
top level: BBa_K1375002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1375024/1

BBa_I13401
type: Component
persistent identity: component2418718
display id: component2418718
version: 1
top level: BBa_K1375002
access: public
definition: https://synbiohub.org/public/igem/BBa_I13401/1

BBa_I13401
type: SequenceAnnotation
persistent identity: annotation2418718
display id: annotation2418718
version: 1
top level: BBa_K1375002
location: https://synbiohub.org/public/igem/BBa_K1375002/annotation2418718/range2418718/1
component: BBa_I13401

https://synbiohub.org/public/igem/BBa_K1375002/annotation2418718/range2418718/1
type: Range
persistent identity: range2418718
display id: range2418718
version: 1
direction: direction/reverse
top level: BBa_K1375002
start: 82
end: 938
orientation: inline

BBa_K1375024
type: SequenceAnnotation
persistent identity: annotation2418710
display id: annotation2418710
version: 1
top level: BBa_K1375002
location: https://synbiohub.org/public/igem/BBa_K1375002/annotation2418710/range2418710/1
component: BBa_K1375024

https://synbiohub.org/public/igem/BBa_K1375002/annotation2418710/range2418710/1
type: Range
persistent identity: range2418710
display id: range2418710
version: 1
direction: direction/reverse
top level: BBa_K1375002
start: 1
end: 75
orientation: inline

BBa_K1331001
type: ComponentDefinition
persistent identity: BBa K1331001
display id: BBa_K1331001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1706_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlA together with RhlB encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa.
mutable notes: A mutation has been done to remove the PstI restriction site contained in the coding sequence.
mutable provenance: Pseudomonas aeruginosa
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: noncoding sequence
sequence annotation: rhlA
sequence: https://synbiohub.org/public/igem/BBa_K1331001_sequence/1

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2427978
display id: annotation2427978
version: 1
top level: BBa_K1331001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331001/annotation2427978/range2427978/1

https://synbiohub.org/public/igem/BBa_K1331001/annotation2427978/range2427978/1
type: Range
persistent identity: range2427978
display id: range2427978
version: 1
direction: direction/forward
top level: BBa_K1331001
start: 889
end: 947
orientation: inline

rhlA
type: SequenceAnnotation
persistent identity: annotation2386609
display id: annotation2386609
version: 1
top level: BBa_K1331001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331001/annotation2386609/range2386609/1

https://synbiohub.org/public/igem/BBa_K1331001/annotation2386609/range2386609/1
type: Range
persistent identity: range2386609
display id: range2386609
version: 1
direction: direction/forward
top level: BBa_K1331001
start: 1
end: 888
orientation: inline

BBa_K1470007
type: ComponentDefinition
persistent identity: BBa K1470007
display id: BBa_K1470007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cas9-Nickase
creator: Dennis Zimmer
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:39Z
discontinued: false
dominant: false
group u list: _1849_
m user id: 0
owner id: 20942
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cas9-Nickase
mutable notes: ...
mutable provenance: iGEM Team Freiburg 2013 Bba_xxx
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1470007_sequence/1

1018
type: ComponentDefinition
persistent identity: BBa K1344000
display id: BBa_K1344000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 1018 synthetic cationic peptide
creator: UI Indonesia 2014
created: 2014-10-03T11:00:00Z
modified: 2015-05-08T01:10:00Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1719_
m user id: 0
owner id: 20150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Peptide 1018 is a synthetic cationic peptides, derived from natural peptides such as the human cathelicidin LL-37 and the bovine peptide indolicidin. This part work as biofilm inhibitory compounds. Anti-biofilm peptides are similar to cationic antimicrobial peptides (which are active against planktonic bacteria), comprising both cationic and hydrophobic amino acids.
mutable notes: This part will have broad spectrum effect on gram-negative and gram-positive bacterias
mutable provenance: This part is synthetized.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATG
sequence annotation: Stop
sequence annotation: 1018
sequence: https://synbiohub.org/public/igem/BBa_K1344000_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2393871
display id: annotation2393871
version: 1
top level: 1018
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1344000/annotation2393871/range2393871/1

https://synbiohub.org/public/igem/BBa_K1344000/annotation2393871/range2393871/1
type: Range
persistent identity: range2393871
display id: range2393871
version: 1
direction: direction/forward
top level: 1018
start: 1
end: 1
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2393870
display id: annotation2393870
version: 1
top level: 1018
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1344000/annotation2393870/range2393870/1

https://synbiohub.org/public/igem/BBa_K1344000/annotation2393870/range2393870/1
type: Range
persistent identity: range2393870
display id: range2393870
version: 1
direction: direction/forward
top level: 1018
start: 40
end: 45
orientation: inline

1018
type: SequenceAnnotation
persistent identity: annotation2393869
display id: annotation2393869
version: 1
top level: 1018
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1344000/annotation2393869/range2393869/1

https://synbiohub.org/public/igem/BBa_K1344000/annotation2393869/range2393869/1
type: Range
persistent identity: range2393869
display id: range2393869
version: 1
direction: direction/forward
top level: 1018
start: 1
end: 45
orientation: inline

BBa_K1399001
type: ComponentDefinition
persistent identity: BBa K1399001
display id: BBa_K1399001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with LVA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-17T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added LVA-ssrA degradation tag. The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. This tag is commonly attached to repressor proteins for use in various gene networks (e.g., oscillators). The tag encodes peptide sequence AANDENYALVA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LVA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations; protein stability; Km of binding to the protease; temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction sites. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LVA in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA-ssrA tag
sequence annotation: cds
sequence annotation: stop
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399001_sequence/1

LVA-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383874
display id: annotation2383874
version: 1
top level: BBa_K1399001
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383874/range2383874/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383874/range2383874/1
type: Range
persistent identity: range2383874
display id: range2383874
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 676
end: 708
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2383873
display id: annotation2383873
version: 1
top level: BBa_K1399001
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383873/range2383873/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383873/range2383873/1
type: Range
persistent identity: range2383873
display id: range2383873
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 4
end: 675
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383875
display id: annotation2383875
version: 1
top level: BBa_K1399001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383875/range2383875/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383875/range2383875/1
type: Range
persistent identity: range2383875
display id: range2383875
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 709
end: 711
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383876
display id: annotation2383876
version: 1
top level: BBa_K1399001
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383876/range2383876/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383876/range2383876/1
type: Range
persistent identity: range2383876
display id: range2383876
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 712
end: 714
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383872
display id: annotation2383872
version: 1
top level: BBa_K1399001
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399001/annotation2383872/range2383872/1

https://synbiohub.org/public/igem/BBa_K1399001/annotation2383872/range2383872/1
type: Range
persistent identity: range2383872
display id: range2383872
version: 1
direction: direction/forward
top level: BBa_K1399001
start: 1
end: 3
orientation: inline

BBa_K1450012
type: ComponentDefinition
persistent identity: BBa K1450012
display id: BBa_K1450012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNA Polymerase and DNA Polymerase
creator: Joe Ho
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:28Z
discontinued: false
dominant: false
group u list: _1828_
m user id: 0
owner id: 9774
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the composite T7 RNA Polymerase and DNA Polymerase
mutable notes: None
mutable provenance: T7 Phage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_I13453
component: BBa_B0034
component: BBa_K1450001
component: BBa_K1450000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1450000
sequence annotation: BBa_B0015
sequence annotation: BBa_K1450001
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1450012_sequence/1

BBa_B0015
type: Component
persistent identity: component2411835
display id: component2411835
version: 1
top level: BBa_K1450012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I13453
type: Component
persistent identity: component2411836
display id: component2411836
version: 1
top level: BBa_K1450012
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0034
type: Component
persistent identity: component2411827
display id: component2411827
version: 1
top level: BBa_K1450012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1450001
type: Component
persistent identity: component2411828
display id: component2411828
version: 1
top level: BBa_K1450012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1450001/1

BBa_K1450000
type: Component
persistent identity: component2411839
display id: component2411839
version: 1
top level: BBa_K1450012
access: public
definition: https://synbiohub.org/public/igem/BBa_K1450000/1

BBa_B0015
type: Component
persistent identity: component2411846
display id: component2411846
version: 1
top level: BBa_K1450012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0034
type: Component
persistent identity: component2411838
display id: component2411838
version: 1
top level: BBa_K1450012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13453
type: Component
persistent identity: component2411825
display id: component2411825
version: 1
top level: BBa_K1450012
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2411838
display id: annotation2411838
version: 1
top level: BBa_K1450012
location: https://synbiohub.org/public/igem/BBa_K1450012/annotation2411838/range2411838/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1450012/annotation2411838/range2411838/1
type: Range
persistent identity: range2411838
display id: range2411838
version: 1
direction: direction/reverse
top level: BBa_K1450012
start: 3095
end: 3106
orientation: inline

BBa_K1450000
type: SequenceAnnotation
persistent identity: annotation2411839
display id: annotation2411839
version: 1
top level: BBa_K1450012
location: https://synbiohub.org/public/igem/BBa_K1450012/annotation2411839/range2411839/1
component: BBa_K1450000

https://synbiohub.org/public/igem/BBa_K1450012/annotation2411839/range2411839/1
type: Range
persistent identity: range2411839
display id: range2411839
version: 1
direction: direction/reverse
top level: BBa_K1450012
start: 3113
end: 5230
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2411846
display id: annotation2411846
version: 1
top level: BBa_K1450012
location: https://synbiohub.org/public/igem/BBa_K1450012/annotation2411846/range2411846/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1450012/annotation2411846/range2411846/1
type: Range
persistent identity: range2411846
display id: range2411846
version: 1
direction: direction/reverse
top level: BBa_K1450012
start: 5239
end: 5367
orientation: inline

BBa_K1450001
type: SequenceAnnotation
persistent identity: annotation2411828
display id: annotation2411828
version: 1
top level: BBa_K1450012
location: https://synbiohub.org/public/igem/BBa_K1450012/annotation2411828/range2411828/1
component: BBa_K1450001

https://synbiohub.org/public/igem/BBa_K1450012/annotation2411828/range2411828/1
type: Range
persistent identity: range2411828
display id: range2411828
version: 1
direction: direction/reverse
top level: BBa_K1450012
start: 157
end: 2811
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2411827
display id: annotation2411827
version: 1
top level: BBa_K1450012
location: https://synbiohub.org/public/igem/BBa_K1450012/annotation2411827/range2411827/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1450012/annotation2411827/range2411827/1
type: Range
persistent identity: range2411827
display id: range2411827
version: 1
direction: direction/reverse
top level: BBa_K1450012
start: 139
end: 150
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2411836
display id: annotation2411836
version: 1
top level: BBa_K1450012
location: https://synbiohub.org/public/igem/BBa_K1450012/annotation2411836/range2411836/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1450012/annotation2411836/range2411836/1
type: Range
persistent identity: range2411836
display id: range2411836
version: 1
direction: direction/reverse
top level: BBa_K1450012
start: 2957
end: 3086
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2411835
display id: annotation2411835
version: 1
top level: BBa_K1450012
location: https://synbiohub.org/public/igem/BBa_K1450012/annotation2411835/range2411835/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1450012/annotation2411835/range2411835/1
type: Range
persistent identity: range2411835
display id: range2411835
version: 1
direction: direction/reverse
top level: BBa_K1450012
start: 2820
end: 2948
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2411825
display id: annotation2411825
version: 1
top level: BBa_K1450012
location: https://synbiohub.org/public/igem/BBa_K1450012/annotation2411825/range2411825/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1450012/annotation2411825/range2411825/1
type: Range
persistent identity: range2411825
display id: range2411825
version: 1
direction: direction/reverse
top level: BBa_K1450012
start: 1
end: 130
orientation: inline

BBa_K1321138
type: ComponentDefinition
persistent identity: BBa K1321138
display id: BBa_K1321138
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Metallothionein SmtA with T7 promoter
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1319106
component: BBa_K1321011
component: BBa_K1321338
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321011
sequence: https://synbiohub.org/public/igem/BBa_K1321138_sequence/1

BBa_K1319106
type: Component
persistent identity: component2414815
display id: component2414815
version: 1
top level: BBa_K1321138
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321011
type: Component
persistent identity: component2414817
display id: component2414817
version: 1
top level: BBa_K1321138
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_K1321338
type: Component
persistent identity: component2414813
display id: component2414813
version: 1
top level: BBa_K1321138
access: public
definition: BBa_K1321338

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2414813
display id: annotation2414813
version: 1
top level: BBa_K1321138
location: https://synbiohub.org/public/igem/BBa_K1321138/annotation2414813/range2414813/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321138/annotation2414813/range2414813/1
type: Range
persistent identity: range2414813
display id: range2414813
version: 1
direction: direction/reverse
top level: BBa_K1321138
start: 1
end: 43
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2414815
display id: annotation2414815
version: 1
top level: BBa_K1321138
location: https://synbiohub.org/public/igem/BBa_K1321138/annotation2414815/range2414815/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321138/annotation2414815/range2414815/1
type: Range
persistent identity: range2414815
display id: range2414815
version: 1
direction: direction/reverse
top level: BBa_K1321138
start: 44
end: 58
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414817
display id: annotation2414817
version: 1
top level: BBa_K1321138
location: https://synbiohub.org/public/igem/BBa_K1321138/annotation2414817/range2414817/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321138/annotation2414817/range2414817/1
type: Range
persistent identity: range2414817
display id: range2414817
version: 1
direction: direction/reverse
top level: BBa_K1321138
start: 59
end: 223
orientation: inline

BBa_K1351033
type: ComponentDefinition
persistent identity: BBa K1351033
display id: BBa_K1351033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LMU Bacillus RBS collection 6
creator: Florian Riemer
created: 2014-10-05T11:00:00Z
modified: 2015-06-01T08:10:31Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20062
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: "This part was generated in a modified version of RFC25, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGATGGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Stop codons are underlined."
mutable notes: The LMU Bacillus RBS collection is based on a wobble-library with the sequence ARRRRRRGATA and the theoretical translation initiation rates of the RBS in this library, calculated by the salis lab's RBS calculator (https://salis.psu.edu/software/). The 7 RBS submitted as BioBricks are the ones that offer the best coverage of translation initiation rate space, 1 being the optimal Bacillus RBS and 7 being the weakest.
mutable provenance: Synthetic
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K1351033_sequence/1

BBa_K1493510
type: ComponentDefinition
persistent identity: BBa K1493510
display id: BBa_K1493510
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRHA+cIλ
creator: Michiel Herpers
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_P0451
component: BBa_K914003
sequence annotation: BBa_P0451
sequence annotation: BBa_K914003
sequence: https://synbiohub.org/public/igem/BBa_K1493510_sequence/1

BBa_P0451
type: Component
persistent identity: component2396335
display id: component2396335
version: 1
top level: BBa_K1493510
access: public
definition: https://synbiohub.org/public/igem/BBa_P0451/1

BBa_K914003
type: Component
persistent identity: component2396322
display id: component2396322
version: 1
top level: BBa_K1493510
access: public
definition: https://synbiohub.org/public/igem/BBa_K914003/1

BBa_P0451
type: SequenceAnnotation
persistent identity: annotation2396335
display id: annotation2396335
version: 1
top level: BBa_K1493510
location: https://synbiohub.org/public/igem/BBa_K1493510/annotation2396335/range2396335/1
component: BBa_P0451

https://synbiohub.org/public/igem/BBa_K1493510/annotation2396335/range2396335/1
type: Range
persistent identity: range2396335
display id: range2396335
version: 1
direction: direction/reverse
top level: BBa_K1493510
start: 131
end: 1060
orientation: inline

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2396322
display id: annotation2396322
version: 1
top level: BBa_K1493510
location: https://synbiohub.org/public/igem/BBa_K1493510/annotation2396322/range2396322/1
component: BBa_K914003

https://synbiohub.org/public/igem/BBa_K1493510/annotation2396322/range2396322/1
type: Range
persistent identity: range2396322
display id: range2396322
version: 1
direction: direction/reverse
top level: BBa_K1493510
start: 1
end: 122
orientation: inline

BBa_K1362050
type: ComponentDefinition
persistent identity: BBa K1362050
display id: BBa_K1362050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intein protease - T7RBS-His-SspDnaBC
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22911
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1362090
component: BBa_K1362251
sequence annotation: BBa_K1362251
sequence annotation: BBa_K1362090
sequence: https://synbiohub.org/public/igem/BBa_K1362050_sequence/1

BBa_K1362090
type: Component
persistent identity: component2409648
display id: component2409648
version: 1
top level: BBa_K1362050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362251
type: Component
persistent identity: component2409656
display id: component2409656
version: 1
top level: BBa_K1362050
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362251/1

BBa_K1362251
type: SequenceAnnotation
persistent identity: annotation2409656
display id: annotation2409656
version: 1
top level: BBa_K1362050
location: https://synbiohub.org/public/igem/BBa_K1362050/annotation2409656/range2409656/1
component: BBa_K1362251

https://synbiohub.org/public/igem/BBa_K1362050/annotation2409656/range2409656/1
type: Range
persistent identity: range2409656
display id: range2409656
version: 1
direction: direction/reverse
top level: BBa_K1362050
start: 36
end: 500
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2409648
display id: annotation2409648
version: 1
top level: BBa_K1362050
location: https://synbiohub.org/public/igem/BBa_K1362050/annotation2409648/range2409648/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362050/annotation2409648/range2409648/1
type: Range
persistent identity: range2409648
display id: range2409648
version: 1
direction: direction/reverse
top level: BBa_K1362050
start: 1
end: 29
orientation: inline

BBa_K1355002
type: ComponentDefinition
persistent identity: BBa K1355002
display id: BBa_K1355002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury ions detector device
creator: Luna Barroco de Lacerda
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 0
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: We used the biobrick (BBa_E0840) available in the database of the iGEM since 2004 to be connected to our ???essential biobrick??? (BBa_K1355001) and - together - form a system capable of biodetect mercury in bacteria. In the presence of mercury, The MerR protein will bind to it and MerT, MerP and the Green Fluorescent Protein (GFP) protein will be expressed and the biodetection will begin! The MerP and MerT protein are responsible for the transport of mercury from the periplasm to the cytoplasm and the GFP will act as rep??rter to alerting the presence of mercury in the middle and the higher concentration of mercury is, higher will be the GFP expression. And If there is no mercury in the middle, the synthesis of MerT, MerP and GFP protein will be repressed by the MerR repressor protein.
mutable notes: For this construct, we followed these steps: Extraction of plasmid DNA and DNA quantification of bacteria transformed with the Essential Biobrick (BBa_K1355001) and the bacteria transformed with the GFP Biobrick (BBa_E0840); Verifying the electrophoretic profile of the extracted plasmid DNA; Restriction enzyme digestion for Essential Biobrick with SpeI and PstI and for GFP Biobrick with PstI and XbaI; Checking the electrophoretic profile of digested samples; Fragment of interest purification from the gel; Ligation the gel purification of GFP gene and digested Essential Biobrick; Transformation of ligation in DH5-alpha; Plasmid DNA extraction from bacteria transformed; Check the electrophoretic profile to see results of samples linked (no fragments); Digestion of Essential Biobrick + GFP Biobrick with EcoRI and PstI, aiming to analyze the fragment size to be isolated; Checking the electrophoretic profile of the digested sample to obtain results showing that the isolated fragment is the junction of Essential Biobrick +GFP Biobrick ;
mutable provenance: BBa_K1355001, BBa_E0840
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0840
component: BBa_K1355001
sequence annotation: BBa_E0840
sequence annotation: BBa_K1355001
sequence: https://synbiohub.org/public/igem/BBa_K1355002_sequence/1

BBa_E0840
type: Component
persistent identity: component2415239
display id: component2415239
version: 1
top level: BBa_K1355002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0840/1

BBa_K1355001
type: Component
persistent identity: component2415228
display id: component2415228
version: 1
top level: BBa_K1355002
access: public
definition: https://synbiohub.org/public/igem/BBa_K1355001/1

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2415239
display id: annotation2415239
version: 1
top level: BBa_K1355002
location: https://synbiohub.org/public/igem/BBa_K1355002/annotation2415239/range2415239/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1355002/annotation2415239/range2415239/1
type: Range
persistent identity: range2415239
display id: range2415239
version: 1
direction: direction/reverse
top level: BBa_K1355002
start: 1198
end: 2075
orientation: inline

BBa_K1355001
type: SequenceAnnotation
persistent identity: annotation2415228
display id: annotation2415228
version: 1
top level: BBa_K1355002
location: https://synbiohub.org/public/igem/BBa_K1355002/annotation2415228/range2415228/1
component: BBa_K1355001

https://synbiohub.org/public/igem/BBa_K1355002/annotation2415228/range2415228/1
type: Range
persistent identity: range2415228
display id: range2415228
version: 1
direction: direction/reverse
top level: BBa_K1355002
start: 1
end: 1189
orientation: inline

Smt3-mDNMT
type: ComponentDefinition
persistent identity: BBa K1362500
display id: BBa_K1362500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Smt3-Murine DNMT1(731-1602) fusion protein (with His6)
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:06Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22951
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable provenance: * Smt3: ''Saccharomyces cerevisiae'' * DNMT1: ''Mus musculus''
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1362458
component: BBa_K1362448
component: BBa_J70594
component: BBa_K1362446
component: BBa_K1362449
component: BBa_K1362456
component: BBa_K1362450
component: BBa_K1362429
sequence annotation: BBa_K1362448
sequence annotation: BBa_K1362449
sequence annotation: BBa_K1362450
sequence annotation: BBa_K1362456
sequence annotation: BBa_K1362446
sequence annotation: BBa_K1362458
sequence annotation: BBa_J70594
sequence annotation: BBa_K1362429
sequence: https://synbiohub.org/public/igem/BBa_K1362500_sequence/1

BBa_K1362458
type: Component
persistent identity: component2410949
display id: component2410949
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362458/1

BBa_K1362448
type: Component
persistent identity: component2410952
display id: component2410952
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362448/1

BBa_J70594
type: Component
persistent identity: component2410957
display id: component2410957
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_J70594/1

BBa_K1362446
type: Component
persistent identity: component2410948
display id: component2410948
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362446/1

BBa_K1362449
type: Component
persistent identity: component2410950
display id: component2410950
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362449/1

BBa_K1362456
type: Component
persistent identity: component2410951
display id: component2410951
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362456/1

BBa_K1362450
type: Component
persistent identity: component2410955
display id: component2410955
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362450/1

BBa_K1362429
type: Component
persistent identity: component2410954
display id: component2410954
version: 1
top level: Smt3-mDNMT
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362429/1

BBa_K1362448
type: SequenceAnnotation
persistent identity: annotation2410952
display id: annotation2410952
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410952/range2410952/1
component: BBa_K1362448

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410952/range2410952/1
type: Range
persistent identity: range2410952
display id: range2410952
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 58
end: 72
orientation: inline

BBa_K1362449
type: SequenceAnnotation
persistent identity: annotation2410950
display id: annotation2410950
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410950/range2410950/1
component: BBa_K1362449

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410950/range2410950/1
type: Range
persistent identity: range2410950
display id: range2410950
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 31
end: 39
orientation: inline

BBa_K1362450
type: SequenceAnnotation
persistent identity: annotation2410955
display id: annotation2410955
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410955/range2410955/1
component: BBa_K1362450

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410955/range2410955/1
type: Range
persistent identity: range2410955
display id: range2410955
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 367
end: 2982
orientation: inline

BBa_K1362456
type: SequenceAnnotation
persistent identity: annotation2410951
display id: annotation2410951
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410951/range2410951/1
component: BBa_K1362456

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410951/range2410951/1
type: Range
persistent identity: range2410951
display id: range2410951
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 40
end: 57
orientation: inline

BBa_K1362446
type: SequenceAnnotation
persistent identity: annotation2410948
display id: annotation2410948
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410948/range2410948/1
component: BBa_K1362446

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410948/range2410948/1
type: Range
persistent identity: range2410948
display id: range2410948
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 1
end: 12
orientation: inline

BBa_K1362458
type: SequenceAnnotation
persistent identity: annotation2410949
display id: annotation2410949
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410949/range2410949/1
component: BBa_K1362458

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410949/range2410949/1
type: Range
persistent identity: range2410949
display id: range2410949
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 13
end: 30
orientation: inline

BBa_J70594
type: SequenceAnnotation
persistent identity: annotation2410957
display id: annotation2410957
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410957/range2410957/1
component: BBa_J70594

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410957/range2410957/1
type: Range
persistent identity: range2410957
display id: range2410957
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 2983
end: 2988
orientation: inline

BBa_K1362429
type: SequenceAnnotation
persistent identity: annotation2410954
display id: annotation2410954
version: 1
top level: Smt3-mDNMT
location: https://synbiohub.org/public/igem/BBa_K1362500/annotation2410954/range2410954/1
component: BBa_K1362429

https://synbiohub.org/public/igem/BBa_K1362500/annotation2410954/range2410954/1
type: Range
persistent identity: range2410954
display id: range2410954
version: 1
direction: direction/reverse
top level: Smt3-mDNMT
start: 73
end: 366
orientation: inline

BBa_K1323013
type: ComponentDefinition
persistent identity: BBa K1323013
display id: BBa_K1323013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: aadD - Kanamycin Resistance Gene Coding Sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-28T01:43:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aadD
sequence: https://synbiohub.org/public/igem/BBa_K1323013_sequence/1

aadD
type: SequenceAnnotation
persistent identity: annotation2428783
display id: annotation2428783
version: 1
top level: BBa_K1323013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323013/annotation2428783/range2428783/1

https://synbiohub.org/public/igem/BBa_K1323013/annotation2428783/range2428783/1
type: Range
persistent identity: range2428783
display id: range2428783
version: 1
direction: direction/forward
top level: BBa_K1323013
start: 1
end: 726
orientation: inline

mRdRP
type: ComponentDefinition
persistent identity: BBa K1442101
display id: BBa_K1442101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RdRP Mutant
creator: Dan Goss
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1820_
m user id: 0
owner id: 23055
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: To test the RdRP against a control, we used a deliberately mutated version of itself. To mutate it, we used the restriction enzyme BgLII (code: agatct). This was not thought through but arbitrarily chosen, and resulted in a shift of frame and the introduction of a stop codon (tga).
mutable notes: Principally, our goal with this part was to create a non-functioning version of our RdRP which we could test it against, to ensure it was the RdRP proper that was having the desired effect of replicating our RNA.
mutable provenance: The source from which this mutant is derived is our RdRP proper, part BBa_K1442100.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Stop Codon TGA
sequence annotation: Untranslated Region
sequence: https://synbiohub.org/public/igem/BBa_K1442101_sequence/1

Stop Codon TGA
type: SequenceAnnotation
persistent identity: annotation2396851
display id: annotation2396851
version: 1
top level: mRdRP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1442101/annotation2396851/range2396851/1

https://synbiohub.org/public/igem/BBa_K1442101/annotation2396851/range2396851/1
type: Range
persistent identity: range2396851
display id: range2396851
version: 1
direction: direction/forward
top level: mRdRP
start: 517
end: 519
orientation: inline

Untranslated Region
type: SequenceAnnotation
persistent identity: annotation2396853
display id: annotation2396853
version: 1
top level: mRdRP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1442101/annotation2396853/range2396853/1

https://synbiohub.org/public/igem/BBa_K1442101/annotation2396853/range2396853/1
type: Range
persistent identity: range2396853
display id: range2396853
version: 1
direction: direction/forward
top level: mRdRP
start: 517
end: 868
orientation: inline

BBa_K1330003
type: ComponentDefinition
persistent identity: BBa K1330003
display id: BBa_K1330003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23110
creator: Kristian Jensen
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
group u list: _1705_
m user id: 0
owner id: 20126
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1330003_sequence/1

BBa_K1445001
type: ComponentDefinition
persistent identity: BBa K1445001
display id: BBa_K1445001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Endogenous Type II CRISPR-Cas9 phagemid
creator: Josephina Hendrix
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:10:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1823_
m user id: 0
owner id: 22016
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The M13ori-pCas9 composite part consists of the M13 origin of replication (M13ori) and the pCas9 part (BBa_K1218011) containing a tracrRNA sequence, the cas9 gene, and a minimal CRISPR array. M13ori (BBa_K1445000) is a 500bp noncoding sequence that is recognized by the gene II of the M13 phage, which results in the uptake of the plasmid containing the ori into an assembled phage coat. This part does not contain any complete phage genes so phage production requires a Helper Phagemid such as M13K07. There will be some packaging of Helper Phagemid but their packaging signal weakened so parts containing the M13ori are preferentially packaged. pCas9 contains the native trcrRNA and promoter region upstream of the Cas9 protein. The type II Cas9 protein is the native, active version from streptococcus pyogenes with the exceptrion of a SNP to erase an illegal EcoRI cleavage site. Downstream of the Cas9 protein is a minimal CRISPR array. This minimal array only includes two CRISPR repeats flanking a spacer region. It is this spacer region that determines which DNA sequence is targeted by the Cas9 endonuclease. Spacer sequence can be easily replaced with the protocol found here.
mutable notes: We have experienced some toxic effects by the pCas9 part so we suggest growing part-containing cells in lower concentrations of antibiotic, even if plasmid contains a high copy origin of replication.
mutable provenance: The M13 origin of replication from Litmus28i and part BBa_K1218011 from the registry.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1218011
component: BBa_K1445000
sequence annotation: BBa_K1218011
sequence annotation: BBa_K1445000
sequence: https://synbiohub.org/public/igem/BBa_K1445001_sequence/1

BBa_K1218011
type: Component
persistent identity: component2386529
display id: component2386529
version: 1
top level: BBa_K1445001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1218011/1

BBa_K1445000
type: Component
persistent identity: component2386528
display id: component2386528
version: 1
top level: BBa_K1445001
access: public
definition: BBa_K1445000

BBa_K1218011
type: SequenceAnnotation
persistent identity: annotation2386529
display id: annotation2386529
version: 1
top level: BBa_K1445001
location: https://synbiohub.org/public/igem/BBa_K1445001/annotation2386529/range2386529/1
component: BBa_K1218011

https://synbiohub.org/public/igem/BBa_K1445001/annotation2386529/range2386529/1
type: Range
persistent identity: range2386529
display id: range2386529
version: 1
direction: direction/reverse
top level: BBa_K1445001
start: 538
end: 5617
orientation: inline

BBa_K1445000
type: SequenceAnnotation
persistent identity: annotation2386528
display id: annotation2386528
version: 1
top level: BBa_K1445001
location: https://synbiohub.org/public/igem/BBa_K1445001/annotation2386528/range2386528/1
component: BBa_K1445000

https://synbiohub.org/public/igem/BBa_K1445001/annotation2386528/range2386528/1
type: Range
persistent identity: range2386528
display id: range2386528
version: 1
direction: direction/reverse
top level: BBa_K1445001
start: 1
end: 529
orientation: inline

BBa_K1333200
type: ComponentDefinition
persistent identity: BBa K1333200
display id: BBa_K1333200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lambda cI-LGF2(E75A)
creator: Yanwen Xie
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fuse lambda cI with LGF2 mutated from Glu to Ala at site 75 (the Glu which locates after three continuous Ala behind lambda repressor is at site 68). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.
mutable notes: The LGF2 is mutated from GAA to GCA at site 75.
mutable provenance: It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lambda cI
sequence annotation: ATG start codon
sequence annotation: LGF2(E75A)
sequence annotation: TGA stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1333200_sequence/1

lambda cI
type: SequenceAnnotation
persistent identity: annotation2385724
display id: annotation2385724
version: 1
top level: BBa_K1333200
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385724/range2385724/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385724/range2385724/1
type: Range
persistent identity: range2385724
display id: range2385724
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 1
end: 711
orientation: inline

ATG start codon
type: SequenceAnnotation
persistent identity: annotation2385725
display id: annotation2385725
version: 1
top level: BBa_K1333200
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385725/range2385725/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385725/range2385725/1
type: Range
persistent identity: range2385725
display id: range2385725
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 1
end: 3
orientation: inline

LGF2(E75A)
type: SequenceAnnotation
persistent identity: annotation2385726
display id: annotation2385726
version: 1
top level: BBa_K1333200
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385726/range2385726/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385726/range2385726/1
type: Range
persistent identity: range2385726
display id: range2385726
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 721
end: 840
orientation: inline

TGA stop codon
type: SequenceAnnotation
persistent identity: annotation2385727
display id: annotation2385727
version: 1
top level: BBa_K1333200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1333200/annotation2385727/range2385727/1

https://synbiohub.org/public/igem/BBa_K1333200/annotation2385727/range2385727/1
type: Range
persistent identity: range2385727
display id: range2385727
version: 1
direction: direction/forward
top level: BBa_K1333200
start: 841
end: 843
orientation: inline

BBa_K1460002
type: ComponentDefinition
persistent identity: BBa K1460002
display id: BBa_K1460002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + GST (glutathione-S-transferase)-CRS5 (metallothionein) + Ter
creator: Eric Holmes
created: 2014-08-13T11:00:00Z
modified: 2016-01-13T10:26:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: his part serves as a functional metallothionein for heavy metal binding and conferred resistance to heavy metals when combined with an appropriate promoter. CRS5 gene is the metallothionein that binds to heavy metals and it is fused to GST for stability.
mutable notes: The part contains a KpnI cut site between the biobrick prefix and the RBS that is a result of removing the T7 promoter from part BBa_K1460001.
mutable provenance: This part is BBa_K1460001 with the T7 promoter removed.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: CRS5
sequence annotation: GST
sequence annotation: BBa_J61101
sequence annotation: BBa_B1006
sequence annotation: Linker Sequence
sequence: https://synbiohub.org/public/igem/BBa_K1460002_sequence/1

CRS5
type: SequenceAnnotation
persistent identity: annotation2380990
display id: annotation2380990
version: 1
top level: BBa_K1460002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380990/range2380990/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380990/range2380990/1
type: Range
persistent identity: range2380990
display id: range2380990
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 721
end: 930
orientation: inline

GST
type: SequenceAnnotation
persistent identity: annotation2380988
display id: annotation2380988
version: 1
top level: BBa_K1460002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380988/range2380988/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380988/range2380988/1
type: Range
persistent identity: range2380988
display id: range2380988
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 34
end: 687
orientation: inline

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2380987
display id: annotation2380987
version: 1
top level: BBa_K1460002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380987/range2380987/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380987/range2380987/1
type: Range
persistent identity: range2380987
display id: range2380987
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 14
end: 27
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2380991
display id: annotation2380991
version: 1
top level: BBa_K1460002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380991/range2380991/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380991/range2380991/1
type: Range
persistent identity: range2380991
display id: range2380991
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 931
end: 969
orientation: inline

Linker Sequence
type: SequenceAnnotation
persistent identity: annotation2380989
display id: annotation2380989
version: 1
top level: BBa_K1460002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460002/annotation2380989/range2380989/1

https://synbiohub.org/public/igem/BBa_K1460002/annotation2380989/range2380989/1
type: Range
persistent identity: range2380989
display id: range2380989
version: 1
direction: direction/forward
top level: BBa_K1460002
start: 688
end: 720
orientation: inline

BBa_K1321152
type: ComponentDefinition
persistent identity: BBa K1321152
display id: BBa_K1321152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: metallothionein Fmt fused to CBDcex
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321012
component: BBa_B0105
component: BBa_K1321003
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321012
sequence annotation: BBa_K1321003
sequence: https://synbiohub.org/public/igem/BBa_K1321152_sequence/1

BBa_K1321012
type: Component
persistent identity: component2415037
display id: component2415037
version: 1
top level: BBa_K1321152
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321012/1

BBa_B0105
type: Component
persistent identity: component2415039
display id: component2415039
version: 1
top level: BBa_K1321152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321003
type: Component
persistent identity: component2415040
display id: component2415040
version: 1
top level: BBa_K1321152
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321003/1

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415039
display id: annotation2415039
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415039/range2415039/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415039/range2415039/1
type: Range
persistent identity: range2415039
display id: range2415039
version: 1
direction: direction/reverse
top level: BBa_K1321152
start: 199
end: 204
orientation: inline

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2415037
display id: annotation2415037
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415037/range2415037/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415037/range2415037/1
type: Range
persistent identity: range2415037
display id: range2415037
version: 1
direction: direction/reverse
top level: BBa_K1321152
start: 1
end: 198
orientation: inline

BBa_K1321003
type: SequenceAnnotation
persistent identity: annotation2415040
display id: annotation2415040
version: 1
top level: BBa_K1321152
location: https://synbiohub.org/public/igem/BBa_K1321152/annotation2415040/range2415040/1
component: BBa_K1321003

https://synbiohub.org/public/igem/BBa_K1321152/annotation2415040/range2415040/1
type: Range
persistent identity: range2415040
display id: range2415040
version: 1
direction: direction/reverse
top level: BBa_K1321152
start: 205
end: 531
orientation: inline

BBa_K1399000
type: ComponentDefinition
persistent identity: BBa K1399000
display id: BBa_K1399000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with AAV-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-16T11:00:00Z
modified: 2015-05-08T01:10:15Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part E1010) with added AAV-ssrA degradation tag (part I11012). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYAAAV and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The AAV tag is reported to lead to reasonably fast protein degradation, degrading GFP with rate -0.012 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: 1) ClpXP and ClpAP protease and SspB mediator concentrations; 2) Protein stability; 3) Km of binding to the protease; 4) Temperature [3]. [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3]Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part E1010, tag sequence was obtained from part I11012, but same tag was also usead in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: cds
sequence annotation: AAV-ssrA tag
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399000_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383858
display id: annotation2383858
version: 1
top level: BBa_K1399000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383858/range2383858/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383858/range2383858/1
type: Range
persistent identity: range2383858
display id: range2383858
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 712
end: 714
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2383855
display id: annotation2383855
version: 1
top level: BBa_K1399000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383855/range2383855/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383855/range2383855/1
type: Range
persistent identity: range2383855
display id: range2383855
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 4
end: 675
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383857
display id: annotation2383857
version: 1
top level: BBa_K1399000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383857/range2383857/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383857/range2383857/1
type: Range
persistent identity: range2383857
display id: range2383857
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 709
end: 711
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383856
display id: annotation2383856
version: 1
top level: BBa_K1399000
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383856/range2383856/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383856/range2383856/1
type: Range
persistent identity: range2383856
display id: range2383856
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 676
end: 708
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383854
display id: annotation2383854
version: 1
top level: BBa_K1399000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399000/annotation2383854/range2383854/1

https://synbiohub.org/public/igem/BBa_K1399000/annotation2383854/range2383854/1
type: Range
persistent identity: range2383854
display id: range2383854
version: 1
direction: direction/forward
top level: BBa_K1399000
start: 1
end: 3
orientation: inline

BBa_K1355003
type: ComponentDefinition
persistent identity: BBa K1355003
display id: BBa_K1355003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mercury ions accumulator device
creator: Luna Barroco de Lacerda, Maria Clara Astolfi
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1731_
m user id: 0
owner id: 15499
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: The Bioaccumulative biobrick is composed of our ???essential biobrick??? (BBa_K1355001) + a Metallothionein (BBa_K346004) available in the database of the iGEM since 2010.In the presence of mercury, The MerR protein will bind to it and MerT, MerP and Metal Binding Protein (MBP) proteins are synthesized and bioaccumulation will start! The MerP and MerT protein are responsible for the transport of mercury from the periplasm to the cytoplasm, leading him to MBP! The MBP binds to the mercury in the cytoplasm, rendering it inactive.
mutable notes: For this construct, we followed these steps: Extraction of plasmid DNA and DNA quantification of bacteria transformed with the Essential Biobrick (BBa_K1355001) and the bacteria transformed with the MBP Biobrick (BBa_K346004); Verifying the electrophoretic profile of the extracted plasmid DNA; Restriction enzyme digestion for Essential Biobrick with SpeI and PstI and for MBP Biobrick with PstI and XbaI; Checking the electrophoretic profile of digested samples; Purification of the Fragment of interest from the gel; Ligation the gel purification of MBP gene and digested Essential Biobrick; Transformation of ligation in DH5-alpha; Plasmid DNA extraction from bacteria transformed; Check the electrophoretic profile to see results of samples linked (no fragments); Digestion of Essential Biobrick + MBP Biobrick with EcoRI and PstI, aiming to analyze the fragment size to be isolated; Checking the electrophoretic profile of the digested sample to obtain results showing that the isolated fragment is the junction of Essential Biobrick +MBP Biobrick ;
mutable provenance: BBa_K1355001; BBa_K346004
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K346004
component: BBa_K1355001
sequence annotation: BBa_K346004
sequence annotation: BBa_K1355001
sequence: https://synbiohub.org/public/igem/BBa_K1355003_sequence/1

BBa_K346004
type: Component
persistent identity: component2415272
display id: component2415272
version: 1
top level: BBa_K1355003
access: public
definition: https://synbiohub.org/public/igem/BBa_K346004/1

BBa_K1355001
type: Component
persistent identity: component2415267
display id: component2415267
version: 1
top level: BBa_K1355003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1355001/1

BBa_K346004
type: SequenceAnnotation
persistent identity: annotation2415272
display id: annotation2415272
version: 1
top level: BBa_K1355003
location: https://synbiohub.org/public/igem/BBa_K1355003/annotation2415272/range2415272/1
component: BBa_K346004

https://synbiohub.org/public/igem/BBa_K1355003/annotation2415272/range2415272/1
type: Range
persistent identity: range2415272
display id: range2415272
version: 1
direction: direction/reverse
top level: BBa_K1355003
start: 1198
end: 1676
orientation: inline

BBa_K1355001
type: SequenceAnnotation
persistent identity: annotation2415267
display id: annotation2415267
version: 1
top level: BBa_K1355003
location: https://synbiohub.org/public/igem/BBa_K1355003/annotation2415267/range2415267/1
component: BBa_K1355001

https://synbiohub.org/public/igem/BBa_K1355003/annotation2415267/range2415267/1
type: Range
persistent identity: range2415267
display id: range2415267
version: 1
direction: direction/reverse
top level: BBa_K1355003
start: 1
end: 1189
orientation: inline

BBa_K1460004
type: ComponentDefinition
persistent identity: BBa K1460004
display id: BBa_K1460004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Anderson Promoter + MerT + MerP + ter
creator: Eric Holmes
created: 2014-08-25T11:00:00Z
modified: 2016-01-13T10:25:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1839_
m user id: 4206
owner id: 15649
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a functional coding sequence for the mercury transport proteins merT/merP from P. aeruginosa. This part will result in mercury intolerance due to greater mercury transport into engineered cells.
mutable notes: The Anderson promoter provides strong constitutive expression of merT/merP in E. coli
mutable provenance: Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. merT/merP genes are naturally found in Pseudomonas aeruginosa. This part was synthesized by GenScript in the vector pUC57. The part was then transferred into pSB1C3 using standard cloning procedures.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_J61101
sequence annotation: merT
sequence annotation: BBa_J23102
sequence annotation: merP
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K1460004_sequence/1

BBa_J61101
type: SequenceAnnotation
persistent identity: annotation2381483
display id: annotation2381483
version: 1
top level: BBa_K1460004
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381483/range2381483/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381483/range2381483/1
type: Range
persistent identity: range2381483
display id: range2381483
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 43
end: 56
orientation: inline

merT
type: SequenceAnnotation
persistent identity: annotation2381485
display id: annotation2381485
version: 1
top level: BBa_K1460004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381485/range2381485/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381485/range2381485/1
type: Range
persistent identity: range2381485
display id: range2381485
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 63
end: 413
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2381484
display id: annotation2381484
version: 1
top level: BBa_K1460004
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381484/range2381484/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381484/range2381484/1
type: Range
persistent identity: range2381484
display id: range2381484
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 1
end: 35
orientation: inline

merP
type: SequenceAnnotation
persistent identity: annotation2381486
display id: annotation2381486
version: 1
top level: BBa_K1460004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381486/range2381486/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381486/range2381486/1
type: Range
persistent identity: range2381486
display id: range2381486
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 426
end: 701
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2381487
display id: annotation2381487
version: 1
top level: BBa_K1460004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1460004/annotation2381487/range2381487/1

https://synbiohub.org/public/igem/BBa_K1460004/annotation2381487/range2381487/1
type: Range
persistent identity: range2381487
display id: range2381487
version: 1
direction: direction/forward
top level: BBa_K1460004
start: 702
end: 740
orientation: inline

BBa_K1429001
type: ComponentDefinition
persistent identity: BBa K1429001
display id: BBa_K1429001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Red Fluorescent Protein (RFP) "Rudolph" coding region, intellectual property-free
creator: Ramsez McCall
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1807_
m user id: 0
owner id: 22579
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: An intellectual property free protein coding region which may be used by any scientist to create a biological machine.
mutable notes: n/a
mutable provenance: DNA 2.0 ProteinPaintbox
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1429001_sequence/1

BBa_K1415102
type: ComponentDefinition
persistent identity: BBa K1415102
display id: BBa_K1415102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons+B0034+PBAN(Mamestra brassicae)
creator: HO, TSUNG YU
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part has a constitutive promoter, so our PBAN(Mamestra brassicae) can constitutive express.
mutable notes: No
mutable provenance: Assemble
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K1415002
component: BBa_J23101
sequence annotation: BBa_J23101
sequence annotation: BBa_K1415002
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1415102_sequence/1

BBa_B0034
type: Component
persistent identity: component2393957
display id: component2393957
version: 1
top level: BBa_K1415102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1415002
type: Component
persistent identity: component2393959
display id: component2393959
version: 1
top level: BBa_K1415102
access: public
definition: BBa_K1415002

BBa_J23101
type: Component
persistent identity: component2393955
display id: component2393955
version: 1
top level: BBa_K1415102
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2393955
display id: annotation2393955
version: 1
top level: BBa_K1415102
location: https://synbiohub.org/public/igem/BBa_K1415102/annotation2393955/range2393955/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1415102/annotation2393955/range2393955/1
type: Range
persistent identity: range2393955
display id: range2393955
version: 1
direction: direction/reverse
top level: BBa_K1415102
start: 1
end: 35
orientation: inline

BBa_K1415002
type: SequenceAnnotation
persistent identity: annotation2393959
display id: annotation2393959
version: 1
top level: BBa_K1415102
location: https://synbiohub.org/public/igem/BBa_K1415102/annotation2393959/range2393959/1
component: BBa_K1415002

https://synbiohub.org/public/igem/BBa_K1415102/annotation2393959/range2393959/1
type: Range
persistent identity: range2393959
display id: range2393959
version: 1
direction: direction/reverse
top level: BBa_K1415102
start: 62
end: 170
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393957
display id: annotation2393957
version: 1
top level: BBa_K1415102
location: https://synbiohub.org/public/igem/BBa_K1415102/annotation2393957/range2393957/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1415102/annotation2393957/range2393957/1
type: Range
persistent identity: range2393957
display id: range2393957
version: 1
direction: direction/reverse
top level: BBa_K1415102
start: 44
end: 55
orientation: inline

BBa_K1499402
type: ComponentDefinition
persistent identity: BBa K1499402
display id: BBa_K1499402
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Uncharacterized wasp protein 1 (PdomMRNAr1.2-02758.1)
creator: Ian Hull
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1879_
m user id: 0
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: todo
mutable notes: todo
mutable provenance: todo
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: uncharacterized wasp protein 1
sequence annotation: TAA stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1499402_sequence/1

uncharacterized wasp protein 1
type: SequenceAnnotation
persistent identity: annotation2413135
display id: annotation2413135
version: 1
top level: BBa_K1499402
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1499402/annotation2413135/range2413135/1

https://synbiohub.org/public/igem/BBa_K1499402/annotation2413135/range2413135/1
type: Range
persistent identity: range2413135
display id: range2413135
version: 1
direction: direction/forward
top level: BBa_K1499402
start: 9
end: 713
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2413275
display id: annotation2413275
version: 1
top level: BBa_K1499402
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1499402/annotation2413275/range2413275/1

https://synbiohub.org/public/igem/BBa_K1499402/annotation2413275/range2413275/1
type: Range
persistent identity: range2413275
display id: range2413275
version: 1
direction: direction/forward
top level: BBa_K1499402
start: 714
end: 716
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2413130
display id: annotation2413130
version: 1
top level: BBa_K1499402
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499402/annotation2413130/range2413130/1

https://synbiohub.org/public/igem/BBa_K1499402/annotation2413130/range2413130/1
type: Range
persistent identity: range2413130
display id: range2413130
version: 1
direction: direction/forward
top level: BBa_K1499402
start: 9
end: 11
orientation: inline

BBa_K1481003
type: ComponentDefinition
persistent identity: BBa K1481003
display id: BBa_K1481003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP with R4-tag under arabinose promoter
creator: Przemysław Nuc, Melania Nowicka, Weronika Krzynowek, Jakub Bartoszewicz, Katarzyna Rżosińska
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1861_
m user id: 0
owner id: 17561
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: sfGFP under arabinose promoter with integrase R4
mutable notes: -
mutable provenance: Synthetic + genomic E.Coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: his-tag
sequence annotation: CRP binding site
sequence annotation: O1 signal
sequence annotation: R4-tag
sequence annotation: O2 signal
sequence annotation: -35 signal
sequence annotation: pBAD
sequence annotation: -10 signal
sequence annotation: sfGFP
sequence annotation: AraC
sequence annotation: AraC binding site
sequence: https://synbiohub.org/public/igem/BBa_K1481003_sequence/1

his-tag
type: SequenceAnnotation
persistent identity: annotation2414820
display id: annotation2414820
version: 1
top level: BBa_K1481003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2414820/range2414820/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2414820/range2414820/1
type: Range
persistent identity: range2414820
display id: range2414820
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 1230
end: 1247
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation2414818
display id: annotation2414818
version: 1
top level: BBa_K1481003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2414818/range2414818/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2414818/range2414818/1
type: Range
persistent identity: range2414818
display id: range2414818
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 1111
end: 1121
orientation: inline

O1 signal
type: SequenceAnnotation
persistent identity: annotation2414805
display id: annotation2414805
version: 1
top level: BBa_K1481003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2414805/range2414805/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2414805/range2414805/1
type: Range
persistent identity: range2414805
display id: range2414805
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 909
end: 924
orientation: inline

R4-tag
type: SequenceAnnotation
persistent identity: annotation2415108
display id: annotation2415108
version: 1
top level: BBa_K1481003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2415108/range2415108/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2415108/range2415108/1
type: Range
persistent identity: range2415108
display id: range2415108
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 1962
end: 1973
orientation: inline

O2 signal
type: SequenceAnnotation
persistent identity: annotation2414806
display id: annotation2414806
version: 1
top level: BBa_K1481003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2414806/range2414806/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2414806/range2414806/1
type: Range
persistent identity: range2414806
display id: range2414806
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 1066
end: 1087
orientation: inline

-35 signal
type: SequenceAnnotation
persistent identity: annotation2414833
display id: annotation2414833
version: 1
top level: BBa_K1481003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2414833/range2414833/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2414833/range2414833/1
type: Range
persistent identity: range2414833
display id: range2414833
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 1153
end: 1157
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2414794
display id: annotation2414794
version: 1
top level: BBa_K1481003
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2414794/range2414794/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2414794/range2414794/1
type: Range
persistent identity: range2414794
display id: range2414794
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 880
end: 1217
orientation: inline

-10 signal
type: SequenceAnnotation
persistent identity: annotation2414832
display id: annotation2414832
version: 1
top level: BBa_K1481003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2414832/range2414832/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2414832/range2414832/1
type: Range
persistent identity: range2414832
display id: range2414832
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 1177
end: 1181
orientation: inline

sfGFP
type: SequenceAnnotation
persistent identity: annotation2414804
display id: annotation2414804
version: 1
top level: BBa_K1481003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2414804/range2414804/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2414804/range2414804/1
type: Range
persistent identity: range2414804
display id: range2414804
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 1218
end: 1967
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2414802
display id: annotation2414802
version: 1
top level: BBa_K1481003
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2414802/range2414802/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2414802/range2414802/1
type: Range
persistent identity: range2414802
display id: range2414802
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 1
end: 879
orientation: inline

AraC binding site
type: SequenceAnnotation
persistent identity: annotation2414807
display id: annotation2414807
version: 1
top level: BBa_K1481003
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1481003/annotation2414807/range2414807/1

https://synbiohub.org/public/igem/BBa_K1481003/annotation2414807/range2414807/1
type: Range
persistent identity: range2414807
display id: range2414807
version: 1
direction: direction/forward
top level: BBa_K1481003
start: 1113
end: 1155
orientation: inline

BBa_K1331004
type: ComponentDefinition
persistent identity: BBa K1331004
display id: BBa_K1331004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6
creator: Tianyi Huang
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1706_
m user id: 0
owner id: 20153
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlB together with RhlA encodes Rhamnosyltransferase I for mono-rahmnolipids biosynthesis in Pseudomonas aeruginosa. This part includes bp upstream noncoding gene and bp downstreanm gene of RhlB. Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
mutable notes: Two mutations have been done to remove the PstI restriction sites contained in the coding sequence, which won't change the amino acid sequence.
mutable provenance: Pseudomonas aeruginosa SQ6
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: noncoding sequence
sequence annotation: RhlB
sequence: https://synbiohub.org/public/igem/BBa_K1331004_sequence/1

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2434208
display id: annotation2434208
version: 1
top level: BBa_K1331004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434208/range2434208/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434208/range2434208/1
type: Range
persistent identity: range2434208
display id: range2434208
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 1
end: 6
orientation: inline

RhlB
type: SequenceAnnotation
persistent identity: annotation2434209
display id: annotation2434209
version: 1
top level: BBa_K1331004
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434209/range2434209/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434209/range2434209/1
type: Range
persistent identity: range2434209
display id: range2434209
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 7
end: 1287
orientation: inline

noncoding sequence
type: SequenceAnnotation
persistent identity: annotation2434210
display id: annotation2434210
version: 1
top level: BBa_K1331004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1331004/annotation2434210/range2434210/1

https://synbiohub.org/public/igem/BBa_K1331004/annotation2434210/range2434210/1
type: Range
persistent identity: range2434210
display id: range2434210
version: 1
direction: direction/forward
top level: BBa_K1331004
start: 1288
end: 1307
orientation: inline

BBa_K1415007
type: ComponentDefinition
persistent identity: BBa K1415007
display id: BBa_K1415007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PBAN (Adoxophyes sp.)
creator: HO, TSUNG YU
created: 2014-10-02T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Leafrollers (Adoxophyes sp.) Spread: The obliquebanded leafroller (OBLR) is native to and widely distributed throughout temperate North America. Characteristics: hatched larvae have a yellowish green body and a black head and thoracic shield. Mature larvae are 20 to 25 mm in length and the head and thoracic shield may be either black or various shades of brown. Damage: Leafrollers, the larvae of certain tortricid moths, often feed and pupate within the protection of rolled-up leaves. Several species can cause problems on fruit and ornamental trees in California. The fruittree leafroller, Archips argyrospila, is the most common leafroller pest in landscapes throughout the state. It occurs on many ornamental trees???including ash, birch, California buckeye, box elder, elm, locust, maple, poplar, rose, and willow???and is particularly damaging to deciduous and live oaks. It also attacks numerous fruit and nut trees including almond, apple, apricot, caneberries, cherry, citrus, pear, plum, prune, quince, and walnut. Control(1) Several parasites attack OBLR larvae but do not adequately control the pest.(2) Apply sprays during June to kill the first summer brood adults and newly hatching larvae.
mutable notes: No
mutable provenance: Artificial synthesis
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PBAN (Adoxophyes sp.)
sequence: https://synbiohub.org/public/igem/BBa_K1415007_sequence/1

PBAN (Adoxophyes sp.)
type: SequenceAnnotation
persistent identity: annotation2392715
display id: annotation2392715
version: 1
top level: BBa_K1415007
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1415007/annotation2392715/range2392715/1

https://synbiohub.org/public/igem/BBa_K1415007/annotation2392715/range2392715/1
type: Range
persistent identity: range2392715
display id: range2392715
version: 1
direction: direction/forward
top level: BBa_K1415007
start: 1
end: 103
orientation: inline

BBa_K1399008
type: ComponentDefinition
persistent identity: BBa K1399008
display id: BBa_K1399008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with DAS-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added DAS-ssrA degradation tag (see part BBa_M0052). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. SsrA tags encode peptide sequence that is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The DAS tag encodes peptide sequence AANDENYADAS is reported to have low affinity to ClpX thus its mediated degradation very much depends on the concentration of SspB (ClpX mediator).[1] However, be aware that exact protein degradation rate is influenced by multiple other factors: ClpXP and ClpAP protease concentrations, protein stability, Km of binding to the protease, temperature [3]. ===References=== [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additional residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (DAS in this case) are recognized by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E0040, the tag sequence was obtained from part BBa_M0052.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: GFP (mut3b)
sequence annotation: DAS-ssrA tag
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399008_sequence/1

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GFP (mut3b)
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DAS-ssrA tag
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BBa_K1321353
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display id: BBa_K1321353
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NiBP fused to CBDclos driven by T7
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:32:03Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
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sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
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component: BBa_K1321343
component: BBa_K1319106
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sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321338
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BBa_K1321104
type: ComponentDefinition
persistent identity: BBa K1321104
display id: BBa_K1321104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to Phytochelatin (PC) EC20 with T7 promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CBD4+PhytoC
mutable notes: CBD4+PhytoC
mutable provenance: CBD4+PhytoC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321002
component: BBa_K1321338
component: BBa_B0105
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sequence annotation: BBa_K1319106
sequence annotation: BBa_K1321005
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sequence annotation: BBa_K1321338
sequence: https://synbiohub.org/public/igem/BBa_K1321104_sequence/1

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type: Component
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definition: BBa_K1321338

BBa_B0105
type: Component
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access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321005
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display id: component2420135
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type: SequenceAnnotation
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BBa_B0105
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BBa_K1321338
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type: Range
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BBa_K1493540
type: ComponentDefinition
persistent identity: BBa K1493540
display id: BBa_K1493540
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRHA+cIλ+LacI
creator: Michiel Herpers
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_P0412
component: BBa_K914003
component: BBa_S0103
sequence annotation: BBa_P0412
sequence annotation: BBa_K914003
sequence annotation: BBa_S0103
sequence: https://synbiohub.org/public/igem/BBa_K1493540_sequence/1

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type: ComponentDefinition
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display id: BBa_K1497029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold (S-P)
creator: Tobias Gabriel, Rene Sahm
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
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sample status: In stock
status: status/Available
bookmark: false
mutable description: Scaffold (S-P)
mutable notes: Scaffold (S-P)
mutable provenance: Scaffold (S-P)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497029_sequence/1

BBa_K1493805
type: ComponentDefinition
persistent identity: BBa K1493805
display id: BBa_K1493805
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter with CIλ and Tet operator sites
creator: Max van der Ploeg
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1873_
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status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Tet operator site
sequence annotation: cI lambda operator site
sequence annotation: cI lamda operator site
sequence annotation: promoter
sequence annotation: -10 binding site
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Tet operator site
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cI lambda operator site
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Tet operator site
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cI lamda operator site
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type: Range
persistent identity: range2422557
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start: 25
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promoter
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display id: annotation2422541
version: 1
top level: BBa_K1493805
role: SO:0000167
role: feature/promoter
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type: Range
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direction: direction/forward
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orientation: inline

-10 binding site
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top level: BBa_K1493805
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role: feature/misc
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type: Range
persistent identity: range2422555
display id: range2422555
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direction: direction/forward
top level: BBa_K1493805
start: 61
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orientation: inline

-34 binding site
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display id: annotation2422542
version: 1
top level: BBa_K1493805
role: SO:0000110
role: feature/misc
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type: Range
persistent identity: range2422542
display id: range2422542
version: 1
direction: direction/forward
top level: BBa_K1493805
start: 39
end: 43
orientation: inline

BBa_K1399006
type: ComponentDefinition
persistent identity: BBa K1399006
display id: BBa_K1399006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP (mut3b) with LAA-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: GFP (mut3b) (see part BBa_E0040) with added LAA-ssrA degradation tag (see part BBa_M0050). The tag increases GFP turn-over rate, thus providing better temporal resolution of green fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes E.coli wild type ssrA peptide sequence AANDENYALAA and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The LAA tag is reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute.[2] However, be aware that exact protein degradation rate depends on multiple factors: ClpXP and ClpAP protease and SspB mediator concentrations, protein stability, Km of binding to the protease, temperature [3]. ===References=== [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to GFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additional residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (LAA in this case) are recognized by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: GFP comes from part BBa_E1010, the tag sequence was obtained from part BBa_M0050.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: GFP (mut3b)
sequence annotation: start
sequence annotation: LAA-ssrA
sequence: https://synbiohub.org/public/igem/BBa_K1399006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383921
display id: annotation2383921
version: 1
top level: BBa_K1399006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383921/range2383921/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383921/range2383921/1
type: Range
persistent identity: range2383921
display id: range2383921
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 748
end: 750
orientation: inline

GFP (mut3b)
type: SequenceAnnotation
persistent identity: annotation2383919
display id: annotation2383919
version: 1
top level: BBa_K1399006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383919/range2383919/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383919/range2383919/1
type: Range
persistent identity: range2383919
display id: range2383919
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 4
end: 714
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383918
display id: annotation2383918
version: 1
top level: BBa_K1399006
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383918/range2383918/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383918/range2383918/1
type: Range
persistent identity: range2383918
display id: range2383918
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383922
display id: annotation2383922
version: 1
top level: BBa_K1399006
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383922/range2383922/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383922/range2383922/1
type: Range
persistent identity: range2383922
display id: range2383922
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 751
end: 753
orientation: inline

LAA-ssrA
type: SequenceAnnotation
persistent identity: annotation2383920
display id: annotation2383920
version: 1
top level: BBa_K1399006
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399006/annotation2383920/range2383920/1

https://synbiohub.org/public/igem/BBa_K1399006/annotation2383920/range2383920/1
type: Range
persistent identity: range2383920
display id: range2383920
version: 1
direction: direction/forward
top level: BBa_K1399006
start: 715
end: 747
orientation: inline

BBa_K1375020
type: ComponentDefinition
persistent identity: BBa K1375020
display id: BBa_K1375020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Prhl/las +RBS+amilGFP
creator: Zheng Xu
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
group u list: _1752_
m user id: 0
owner id: 18474
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a regulated promoter with a yellow chromoprotein
mutable notes: NO
mutable provenance: K1375024 K592010
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1375024
component: BBa_K592010
sequence annotation: BBa_K1375024
sequence annotation: BBa_K592010
sequence: https://synbiohub.org/public/igem/BBa_K1375020_sequence/1

BBa_K1375024
type: Component
persistent identity: component2416887
display id: component2416887
version: 1
top level: BBa_K1375020
access: public
definition: https://synbiohub.org/public/igem/BBa_K1375024/1

BBa_K592010
type: Component
persistent identity: component2416889
display id: component2416889
version: 1
top level: BBa_K1375020
access: public
definition: https://synbiohub.org/public/igem/BBa_K592010/1

BBa_K1375024
type: SequenceAnnotation
persistent identity: annotation2416887
display id: annotation2416887
version: 1
top level: BBa_K1375020
location: https://synbiohub.org/public/igem/BBa_K1375020/annotation2416887/range2416887/1
component: BBa_K1375024

https://synbiohub.org/public/igem/BBa_K1375020/annotation2416887/range2416887/1
type: Range
persistent identity: range2416887
display id: range2416887
version: 1
direction: direction/reverse
top level: BBa_K1375020
start: 1
end: 75
orientation: inline

BBa_K592010
type: SequenceAnnotation
persistent identity: annotation2416889
display id: annotation2416889
version: 1
top level: BBa_K1375020
location: https://synbiohub.org/public/igem/BBa_K1375020/annotation2416889/range2416889/1
component: BBa_K592010

https://synbiohub.org/public/igem/BBa_K1375020/annotation2416889/range2416889/1
type: Range
persistent identity: range2416889
display id: range2416889
version: 1
direction: direction/reverse
top level: BBa_K1375020
start: 82
end: 780
orientation: inline

BBa_K1333102
type: ComponentDefinition
persistent identity: BBa K1333102
display id: BBa_K1333102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS umuC
creator: Yanwen Xie
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: umuc
mutable notes: umuc
mutable provenance: umuc
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1333107
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1333107
sequence: https://synbiohub.org/public/igem/BBa_K1333102_sequence/1

BBa_K1333107
type: Component
persistent identity: component2429518
display id: component2429518
version: 1
top level: BBa_K1333102
access: public
definition: BBa_K1333107

BBa_B0034
type: Component
persistent identity: component2429516
display id: component2429516
version: 1
top level: BBa_K1333102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2429516
display id: annotation2429516
version: 1
top level: BBa_K1333102
location: https://synbiohub.org/public/igem/BBa_K1333102/annotation2429516/range2429516/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1333102/annotation2429516/range2429516/1
type: Range
persistent identity: range2429516
display id: range2429516
version: 1
direction: direction/reverse
top level: BBa_K1333102
start: 1
end: 12
orientation: inline

BBa_K1333107
type: SequenceAnnotation
persistent identity: annotation2429518
display id: annotation2429518
version: 1
top level: BBa_K1333102
location: https://synbiohub.org/public/igem/BBa_K1333102/annotation2429518/range2429518/1
component: BBa_K1333107

https://synbiohub.org/public/igem/BBa_K1333102/annotation2429518/range2429518/1
type: Range
persistent identity: range2429518
display id: range2429518
version: 1
direction: direction/reverse
top level: BBa_K1333102
start: 19
end: 1287
orientation: inline

BBa_K1330001
type: ComponentDefinition
persistent identity: BBa K1330001
display id: BBa_K1330001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spinach2.1 flanked by tRNALys3 with terminator BBa_B0052
creator: Kristian Jensen
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1705_
m user id: 0
owner id: 20126
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Test2
mutable notes: Test
mutable provenance: Test
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
sequence: https://synbiohub.org/public/igem/BBa_K1330001_sequence/1

BBa_K1323012
type: ComponentDefinition
persistent identity: BBa K1323012
display id: BBa_K1323012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spc - Spectinomycin Resistance Gene Coding Sequence
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2016-01-28T01:43:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1698_
m user id: 4206
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: spc
sequence: https://synbiohub.org/public/igem/BBa_K1323012_sequence/1

spc
type: SequenceAnnotation
persistent identity: annotation2428780
display id: annotation2428780
version: 1
top level: BBa_K1323012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323012/annotation2428780/range2428780/1

https://synbiohub.org/public/igem/BBa_K1323012/annotation2428780/range2428780/1
type: Range
persistent identity: range2428780
display id: range2428780
version: 1
direction: direction/forward
top level: BBa_K1323012
start: 1
end: 783
orientation: inline

BBa_K1343022
type: ComponentDefinition
persistent identity: BBa K1343022
display id: BBa_K1343022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux->amilCP->tetR
creator: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1718_
m user id: 0
owner id: 20669
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The promoter Plux in induced by the dimer AHL-luxR. As a result, amilCP, a blue chromophoire protein, is synthesized. TetR is a repressor of the promoter Ptet.
mutable notes: r
mutable provenance: BBa_R0062: Plux, BBa_B0030: Strong RBS, BBa_K592009: amilCP, BBa_B0010:T1 (terminator) from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1343022_sequence/1

BBa_K1321347
type: ComponentDefinition
persistent identity: BBa K1321347
display id: BBa_K1321347
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCBD with N-terminal linker in RFC 25
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:53Z
discontinued: true
dominant: false
experience: experience/None
group u list: _1696_
m user id: 0
owner id: 20830
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dCBD
sequence: https://synbiohub.org/public/igem/BBa_K1321347_sequence/1

dCBD
type: SequenceAnnotation
persistent identity: annotation2414642
display id: annotation2414642
version: 1
top level: BBa_K1321347
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321347/annotation2414642/range2414642/1

https://synbiohub.org/public/igem/BBa_K1321347/annotation2414642/range2414642/1
type: Range
persistent identity: range2414642
display id: range2414642
version: 1
direction: direction/forward
top level: BBa_K1321347
start: 1
end: 366
orientation: inline

BBa_K1491015
type: ComponentDefinition
persistent identity: BBa K1491015
display id: BBa_K1491015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Codon Optimized KillerRed
creator: Danielle Peters
created: 2014-08-03T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _1871_
m user id: 0
owner id: 22521
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the codon optimized version of KillerRed (BBa_K1184000). It has been optimized for E. coli by eliminating the rare codons.
mutable notes: Increase the functionality of KillerRed through codon optimization.
mutable provenance: This part was codon optimized from the original KillerRed (BBa_K1184000)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: 6x His Tag
sequence: https://synbiohub.org/public/igem/BBa_K1491015_sequence/1

6x His Tag
type: SequenceAnnotation
persistent identity: annotation2380344
display id: annotation2380344
version: 1
top level: BBa_K1491015
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1491015/annotation2380344/range2380344/1

https://synbiohub.org/public/igem/BBa_K1491015/annotation2380344/range2380344/1
type: Range
persistent identity: range2380344
display id: range2380344
version: 1
direction: direction/forward
top level: BBa_K1491015
start: 718
end: 735
orientation: inline

BBa_K1493300
type: ComponentDefinition
persistent identity: BBa K1493300
display id: BBa_K1493300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: methionine-γ-lyase
creator: Wen Wu
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Converts L-methionine to mathanethiol, ammonium and 2-oxobutanoate
mutable notes: -
mutable provenance: ?
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: methionine-gamma-lyase
sequence annotation: RBS B0034
sequence: https://synbiohub.org/public/igem/BBa_K1493300_sequence/1

methionine-gamma-lyase
type: SequenceAnnotation
persistent identity: annotation2401037
display id: annotation2401037
version: 1
top level: BBa_K1493300
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1493300/annotation2401037/range2401037/1

https://synbiohub.org/public/igem/BBa_K1493300/annotation2401037/range2401037/1
type: Range
persistent identity: range2401037
display id: range2401037
version: 1
direction: direction/forward
top level: BBa_K1493300
start: 18
end: 1296
orientation: inline

RBS B0034
type: SequenceAnnotation
persistent identity: annotation2401036
display id: annotation2401036
version: 1
top level: BBa_K1493300
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1493300/annotation2401036/range2401036/1

https://synbiohub.org/public/igem/BBa_K1493300/annotation2401036/range2401036/1
type: Range
persistent identity: range2401036
display id: range2401036
version: 1
direction: direction/forward
top level: BBa_K1493300
start: 1
end: 12
orientation: inline

BBa_K1554002
type: ComponentDefinition
persistent identity: BBa K1554002
display id: BBa_K1554002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HarFAR
creator: Valencia_UPV team members
created: 2014-09-25T11:00:00Z
modified: 2015-05-28T11:28:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1939_
m user id: 4206
owner id: 22386
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fatty alcohol reductase
mutable notes: BioBrick compatible
mutable provenance: It comes from Helicoverpa armigera.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1554002_sequence/1

GAFP-1
type: ComponentDefinition
persistent identity: BBa K1364007
display id: BBa_K1364007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Antifungal GAFP-1 + Double terminator
creator: Jourdan Camille
created: 2014-10-02T11:00:00Z
modified: 2015-06-08T08:40:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1740_
m user id: 4206
owner id: 21465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: bla bla
mutable notes: bla
mutable provenance: bla
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1364002
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K1364002
sequence: https://synbiohub.org/public/igem/BBa_K1364007_sequence/1

BBa_K1364002
type: Component
persistent identity: component2392847
display id: component2392847
version: 1
top level: GAFP-1
access: public
definition: https://synbiohub.org/public/igem/BBa_K1364002/1

BBa_B0015
type: Component
persistent identity: component2392854
display id: component2392854
version: 1
top level: GAFP-1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2392854
display id: annotation2392854
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364007/annotation2392854/range2392854/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1364007/annotation2392854/range2392854/1
type: Range
persistent identity: range2392854
display id: range2392854
version: 1
direction: direction/reverse
top level: GAFP-1
start: 196
end: 324
orientation: inline

BBa_K1364002
type: SequenceAnnotation
persistent identity: annotation2392847
display id: annotation2392847
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364007/annotation2392847/range2392847/1
component: BBa_K1364002

https://synbiohub.org/public/igem/BBa_K1364007/annotation2392847/range2392847/1
type: Range
persistent identity: range2392847
display id: range2392847
version: 1
direction: direction/reverse
top level: GAFP-1
start: 1
end: 187
orientation: inline

BBa_K1321121
type: ComponentDefinition
persistent identity: BBa K1321121
display id: BBa_K1321121
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Smt metallothionein fused to CBDclos
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0105
component: BBa_K1321011
component: BBa_K1321002
sequence annotation: BBa_K1321011
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321002
sequence: https://synbiohub.org/public/igem/BBa_K1321121_sequence/1

BBa_B0105
type: Component
persistent identity: component2414705
display id: component2414705
version: 1
top level: BBa_K1321121
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321011
type: Component
persistent identity: component2414703
display id: component2414703
version: 1
top level: BBa_K1321121
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_K1321002
type: Component
persistent identity: component2414706
display id: component2414706
version: 1
top level: BBa_K1321121
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414703
display id: annotation2414703
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414703/range2414703/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414703/range2414703/1
type: Range
persistent identity: range2414703
display id: range2414703
version: 1
direction: direction/reverse
top level: BBa_K1321121
start: 1
end: 165
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414705
display id: annotation2414705
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414705/range2414705/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414705/range2414705/1
type: Range
persistent identity: range2414705
display id: range2414705
version: 1
direction: direction/reverse
top level: BBa_K1321121
start: 166
end: 171
orientation: inline

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2414706
display id: annotation2414706
version: 1
top level: BBa_K1321121
location: https://synbiohub.org/public/igem/BBa_K1321121/annotation2414706/range2414706/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321121/annotation2414706/range2414706/1
type: Range
persistent identity: range2414706
display id: range2414706
version: 1
direction: direction/reverse
top level: BBa_K1321121
start: 172
end: 471
orientation: inline

nadE
type: ComponentDefinition
persistent identity: BBa K1373000
display id: BBa_K1373000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: This part encodes NAD synthetase
creator: Wenxuan Chen
created: 2014-08-13T11:00:00Z
modified: 2015-05-08T01:10:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1750_
m user id: 0
owner id: 23360
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The nadE gene encodes Naphthyl Acetamide synthetase which can boot up the NAD+(H) level in the cells facilitating the electron transferring rate in MFCs (Microbial Fuel Cells).
mutable notes: The sequence is a full length ORF containing the initiation codon and the termination codon.
mutable provenance: This part is aquried from genome the E.coli K-12 substrain MG1655.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: nadE
sequence: https://synbiohub.org/public/igem/BBa_K1373000_sequence/1

nadE
type: SequenceAnnotation
persistent identity: annotation2385761
display id: annotation2385761
version: 1
top level: nadE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1373000/annotation2385761/range2385761/1

https://synbiohub.org/public/igem/BBa_K1373000/annotation2385761/range2385761/1
type: Range
persistent identity: range2385761
display id: range2385761
version: 1
direction: direction/forward
top level: nadE
start: 1
end: 828
orientation: inline

BBa_K1321154
type: ComponentDefinition
persistent identity: BBa K1321154
display id: BBa_K1321154
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CBDclos fused to metallothionein Fmt
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:23:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321002
component: BBa_K1321012
component: BBa_B0105
sequence annotation: BBa_K1321002
sequence annotation: BBa_K1321012
sequence annotation: BBa_B0105
sequence: https://synbiohub.org/public/igem/BBa_K1321154_sequence/1

BBa_K1321002
type: Component
persistent identity: component2415053
display id: component2415053
version: 1
top level: BBa_K1321154
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321002/1

BBa_K1321012
type: Component
persistent identity: component2415057
display id: component2415057
version: 1
top level: BBa_K1321154
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321012/1

BBa_B0105
type: Component
persistent identity: component2415055
display id: component2415055
version: 1
top level: BBa_K1321154
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321002
type: SequenceAnnotation
persistent identity: annotation2415053
display id: annotation2415053
version: 1
top level: BBa_K1321154
location: https://synbiohub.org/public/igem/BBa_K1321154/annotation2415053/range2415053/1
component: BBa_K1321002

https://synbiohub.org/public/igem/BBa_K1321154/annotation2415053/range2415053/1
type: Range
persistent identity: range2415053
display id: range2415053
version: 1
direction: direction/reverse
top level: BBa_K1321154
start: 1
end: 300
orientation: inline

BBa_K1321012
type: SequenceAnnotation
persistent identity: annotation2415057
display id: annotation2415057
version: 1
top level: BBa_K1321154
location: https://synbiohub.org/public/igem/BBa_K1321154/annotation2415057/range2415057/1
component: BBa_K1321012

https://synbiohub.org/public/igem/BBa_K1321154/annotation2415057/range2415057/1
type: Range
persistent identity: range2415057
display id: range2415057
version: 1
direction: direction/reverse
top level: BBa_K1321154
start: 307
end: 504
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415055
display id: annotation2415055
version: 1
top level: BBa_K1321154
location: https://synbiohub.org/public/igem/BBa_K1321154/annotation2415055/range2415055/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321154/annotation2415055/range2415055/1
type: Range
persistent identity: range2415055
display id: range2415055
version: 1
direction: direction/reverse
top level: BBa_K1321154
start: 301
end: 306
orientation: inline

BBa_K1323014
type: ComponentDefinition
persistent identity: BBa K1323014
display id: BBa_K1323014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Xylose Inducible Promoter for Staphylococcus aureus
creator: Julia Manalil
created: 2014-10-03T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1698_
m user id: 0
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: XylR promoter
sequence annotation: pT5X
sequence annotation: Removal of EcoRI site (C to T)
sequence annotation: Terminator
sequence annotation: XylR cds
sequence annotation: Putative RBS
sequence: https://synbiohub.org/public/igem/BBa_K1323014_sequence/1

XylR promoter
type: SequenceAnnotation
persistent identity: annotation2417891
display id: annotation2417891
version: 1
top level: BBa_K1323014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1323014/annotation2417891/range2417891/1

https://synbiohub.org/public/igem/BBa_K1323014/annotation2417891/range2417891/1
type: Range
persistent identity: range2417891
display id: range2417891
version: 1
direction: direction/forward
top level: BBa_K1323014
start: 26
end: 54
orientation: inline

pT5X
type: SequenceAnnotation
persistent identity: annotation2417892
display id: annotation2417892
version: 1
top level: BBa_K1323014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1323014/annotation2417892/range2417892/1

https://synbiohub.org/public/igem/BBa_K1323014/annotation2417892/range2417892/1
type: Range
persistent identity: range2417892
display id: range2417892
version: 1
direction: direction/forward
top level: BBa_K1323014
start: 1367
end: 1417
orientation: inline

Removal of EcoRI site (C to T)
type: SequenceAnnotation
persistent identity: annotation2417889
display id: annotation2417889
version: 1
top level: BBa_K1323014
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1323014/annotation2417889/range2417889/1

https://synbiohub.org/public/igem/BBa_K1323014/annotation2417889/range2417889/1
type: Range
persistent identity: range2417889
display id: range2417889
version: 1
direction: direction/forward
top level: BBa_K1323014
start: 1460
end: 1460
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2417890
display id: annotation2417890
version: 1
top level: BBa_K1323014
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1323014/annotation2417890/range2417890/1

https://synbiohub.org/public/igem/BBa_K1323014/annotation2417890/range2417890/1
type: Range
persistent identity: range2417890
display id: range2417890
version: 1
direction: direction/forward
top level: BBa_K1323014
start: 1334
end: 1364
orientation: inline

XylR cds
type: SequenceAnnotation
persistent identity: annotation2417888
display id: annotation2417888
version: 1
top level: BBa_K1323014
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323014/annotation2417888/range2417888/1

https://synbiohub.org/public/igem/BBa_K1323014/annotation2417888/range2417888/1
type: Range
persistent identity: range2417888
display id: range2417888
version: 1
direction: direction/forward
top level: BBa_K1323014
start: 176
end: 1327
orientation: inline

Putative RBS
type: SequenceAnnotation
persistent identity: annotation2428792
display id: annotation2428792
version: 1
top level: BBa_K1323014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1323014/annotation2428792/range2428792/1

https://synbiohub.org/public/igem/BBa_K1323014/annotation2428792/range2428792/1
type: Range
persistent identity: range2428792
display id: range2428792
version: 1
direction: direction/forward
top level: BBa_K1323014
start: 161
end: 166
orientation: inline

BBa_K1351026
type: ComponentDefinition
persistent identity: BBa K1351026
display id: BBa_K1351026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Signal peptide of B. subtilis major autolysin LytC
creator: Mona Dotzler
created: 2014-10-04T11:00:00Z
modified: 2015-06-01T12:56:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Signal peptide (amino acids 1 to 25) of ''Bacillus subtilis'' major autolysin LytC (N-acetylmuramoyl-L-alanine amidase, Uniprot [http://www.uniprot.org/uniprot/Q02114 Q02114]). Targets proteins for secretion via the Sec pathway (Tjalsma ''et. al.'', 2000).
mutable notes: none
mutable provenance: This part was generated by amplification from BioBrick [http://parts.igem.org/Part:BBa_K1351006 BBa_K1351006] with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3. LytC_ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC TTGCGTTCTTATATAAAAGTCCTAACAATGTG LytcSP_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT ATCGGCCAAAGCTGTTGGC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351026_sequence/1

BBa_K1321357
type: ComponentDefinition
persistent identity: BBa K1321357
display id: BBa_K1321357
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP fused to CBDcex driven by LacI
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T09:45:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: asdf
mutable notes: asdf
mutable provenance: asdf
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1321342
component: BBa_J04500
component: BBa_K1319106
sequence annotation: BBa_K1321342
sequence annotation: BBa_K1319106
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_K1321357_sequence/1

BBa_K1321342
type: Component
persistent identity: component2415507
display id: component2415507
version: 1
top level: BBa_K1321357
access: public
definition: BBa_K1321342

BBa_J04500
type: Component
persistent identity: component2415499
display id: component2415499
version: 1
top level: BBa_K1321357
access: public
definition: https://synbiohub.org/public/igem/BBa_J04500/1

BBa_K1319106
type: Component
persistent identity: component2415501
display id: component2415501
version: 1
top level: BBa_K1321357
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321342
type: SequenceAnnotation
persistent identity: annotation2415507
display id: annotation2415507
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415507/range2415507/1
component: BBa_K1321342

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415507/range2415507/1
type: Range
persistent identity: range2415507
display id: range2415507
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 236
end: 1279
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415501
display id: annotation2415501
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415501/range2415501/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415501/range2415501/1
type: Range
persistent identity: range2415501
display id: range2415501
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 221
end: 235
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2415499
display id: annotation2415499
version: 1
top level: BBa_K1321357
location: https://synbiohub.org/public/igem/BBa_K1321357/annotation2415499/range2415499/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_K1321357/annotation2415499/range2415499/1
type: Range
persistent identity: range2415499
display id: range2415499
version: 1
direction: direction/reverse
top level: BBa_K1321357
start: 1
end: 220
orientation: inline

BBa_K1362013
type: ComponentDefinition
persistent identity: BBa K1362013
display id: BBa_K1362013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + Circular λ-Lysozyme with rigid linker
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22920
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1362999
component: BBa_K1362400
component: BBa_K1362421
component: BBa_K1362401
component: BBa_K1362432
component: BBa_K1362090
component: BBa_K1362415
component: BBa_K1362434
component: BBa_K1362433
component: BBa_K1362419
component: BBa_K1362439
component: BBa_G0000
component: BBa_K1362414
sequence annotation: BBa_K1362433
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362401
sequence annotation: BBa_K1362999
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362434
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362439
sequence annotation: BBa_K1362419
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362400
sequence annotation: BBa_K1362432
sequence: https://synbiohub.org/public/igem/BBa_K1362013_sequence/1

BBa_K1362999
type: Component
persistent identity: component2403283
display id: component2403283
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362999/1

BBa_K1362400
type: Component
persistent identity: component2403277
display id: component2403277
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362400/1

BBa_K1362421
type: Component
persistent identity: component2403279
display id: component2403279
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_K1362401
type: Component
persistent identity: component2403270
display id: component2403270
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362401/1

BBa_K1362432
type: Component
persistent identity: component2403273
display id: component2403273
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362432/1

BBa_K1362090
type: Component
persistent identity: component2403267
display id: component2403267
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362415
type: Component
persistent identity: component2403276
display id: component2403276
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362434
type: Component
persistent identity: component2403275
display id: component2403275
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362434/1

BBa_K1362433
type: Component
persistent identity: component2403272
display id: component2403272
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362433/1

BBa_K1362419
type: Component
persistent identity: component2403271
display id: component2403271
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362439
type: Component
persistent identity: component2403274
display id: component2403274
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362439/1

BBa_G0000
type: Component
persistent identity: component2403268
display id: component2403268
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362414
type: Component
persistent identity: component2403269
display id: component2403269
version: 1
top level: BBa_K1362013
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362433
type: SequenceAnnotation
persistent identity: annotation2403272
display id: annotation2403272
version: 1
top level: BBa_K1362013
location: https://synbiohub.org/public/igem/BBa_K1362013/annotation2403272/range2403272/1
component: BBa_K1362433

https://synbiohub.org/public/igem/BBa_K1362013/annotation2403272/range2403272/1
type: Range
persistent identity: range2403272
display id: range2403272
version: 1
direction: direction/reverse
top level: BBa_K1362013
start: 144
end: 152
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2403267
display id: annotation2403267
version: 1
top level: BBa_K1362013
location: https://synbiohub.org/public/igem/BBa_K1362013/annotation2403267/range2403267/1
component: BBa_K1362090

https://synbiohub.org/public/igem/BBa_K1362013/annotation2403267/range2403267/1
type: Range
persistent identity: range2403267
display id: range2403267
version: 1
direction: direction/reverse
top level: BBa_K1362013
start: 1
end: 29
orientation: inline

BBa_K1362401
type: SequenceAnnotation
persistent identity: annotation2403270
display id: annotation2403270
version: 1
top level: BBa_K1362013
location: https://synbiohub.org/public/igem/BBa_K1362013/annotation2403270/range2403270/1
component: BBa_K1362401

https://synbiohub.org/public/igem/BBa_K1362013/annotation2403270/range2403270/1
type: Range
persistent identity: range2403270
display id: range2403270
version: 1
direction: direction/reverse
top level: BBa_K1362013
start: 39
end: 139
orientation: inline

BBa_K1362999
type: SequenceAnnotation
persistent identity: annotation2403283
display id: annotation2403283
version: 1
top level: BBa_K1362013
location: https://synbiohub.org/public/igem/BBa_K1362013/annotation2403283/range2403283/1
component: BBa_K1362999

https://synbiohub.org/public/igem/BBa_K1362013/annotation2403283/range2403283/1
type: Range
persistent identity: range2403283
display id: range2403283
version: 1
direction: direction/reverse
top level: BBa_K1362013
start: 991
end: 1016
orientation: inline

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2403269
display id: annotation2403269
version: 1
top level: BBa_K1362013
location: https://synbiohub.org/public/igem/BBa_K1362013/annotation2403269/range2403269/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362013/annotation2403269/range2403269/1
type: Range
persistent identity: range2403269
display id: range2403269
version: 1
direction: direction/reverse
top level: BBa_K1362013
start: 36
end: 38
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BBa_K1362434
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was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + λ-Lysozyme
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created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
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group u list: _1738_
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mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
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star: false
type: DnaRegion
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BBa_K1321111
type: ComponentDefinition
persistent identity: BBa K1321111
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version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused with CBDclos with LacI promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:19:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
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status: status/Available
bookmark: false
mutable description: PhytoC+CBD4 with LacI
mutable notes: PhytoC+CBD4 with LacI
mutable provenance: PhytoC+CBD4 with LacI
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star: false
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type: ComponentDefinition
persistent identity: BBa K1362060
display id: BBa_K1362060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OmpA surface protein as RFC[105] N-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
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modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
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sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
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BBa_K1399013
type: ComponentDefinition
persistent identity: BBa K1399013
display id: BBa_K1399013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(DAS)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter with RFP reporter tagged with DAS-ssrA degradation tag followed by terminator. The tagged RFP is degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Presence of SspB is crucial for effective protein degradation.
mutable notes: Part was assembled using BrickClip assembly (BBF RFC104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1]
mutable provenance: other biobricks
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: RFP
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: stop
sequence annotation: -35
sequence annotation: stem_loop
sequence annotation: BBa_K1399003
sequence annotation: -10
sequence annotation: BBa_R0010
sequence annotation: BBa_B0015
sequence annotation: BBa_B0010
sequence annotation: polya
sequence annotation: conserved
sequence annotation: T7 TE
sequence annotation: CAP binding site
sequence annotation: start
sequence annotation: LacI binding site
sequence annotation: DAS-ssrA tag
sequence annotation: barcode from BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K1399013_sequence/1

RFP
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top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384022/range2384022/1
type: Range
persistent identity: range2384022
display id: range2384022
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 230
end: 901
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2384013
display id: annotation2384013
version: 1
top level: BBa_K1399013
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384013/range2384013/1
type: Range
persistent identity: range2384013
display id: range2384013
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 88
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2384029
display id: annotation2384029
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384029/range2384029/1
type: Range
persistent identity: range2384029
display id: range2384029
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1062
end: 1102
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2384020
display id: annotation2384020
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384020/range2384020/1
type: Range
persistent identity: range2384020
display id: range2384020
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 209
end: 220
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384025
display id: annotation2384025
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384025/range2384025/1
type: Range
persistent identity: range2384025
display id: range2384025
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 938
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384024
display id: annotation2384024
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384024/range2384024/1
type: Range
persistent identity: range2384024
display id: range2384024
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 935
end: 937
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2384032
display id: annotation2384032
version: 1
top level: BBa_K1399013
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384032/range2384032/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384032/range2384032/1
type: Range
persistent identity: range2384032
display id: range2384032
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1095
end: 1095
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2384015
display id: annotation2384015
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384015/range2384015/1
type: Range
persistent identity: range2384015
display id: range2384015
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 137
end: 142
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2384028
display id: annotation2384028
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384028/range2384028/1
type: Range
persistent identity: range2384028
display id: range2384028
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 985
end: 1028
orientation: inline

BBa_K1399003
type: SequenceAnnotation
persistent identity: annotation2384026
display id: annotation2384026
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384026/range2384026/1
type: Range
persistent identity: range2384026
display id: range2384026
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 227
end: 940
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2384016
display id: annotation2384016
version: 1
top level: BBa_K1399013
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384016/range2384016/1
type: Range
persistent identity: range2384016
display id: range2384016
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 161
end: 166
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2384012
display id: annotation2384012
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384012/range2384012/1
type: Range
persistent identity: range2384012
display id: range2384012
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1
end: 200
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2384033
display id: annotation2384033
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384033/range2384033/1
type: Range
persistent identity: range2384033
display id: range2384033
version: 1
direction: direction/reverse
top level: BBa_K1399013
start: 974
end: 1102
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2384027
display id: annotation2384027
version: 1
top level: BBa_K1399013
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384027/range2384027/1
type: Range
persistent identity: range2384027
display id: range2384027
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 974
end: 1053
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2384031
display id: annotation2384031
version: 1
top level: BBa_K1399013
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384031/range2384031/1
type: Range
persistent identity: range2384031
display id: range2384031
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1089
end: 1102
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2384019
display id: annotation2384019
version: 1
top level: BBa_K1399013
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384019/range2384019/1
type: Range
persistent identity: range2384019
display id: range2384019
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 213
end: 216
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2384030
display id: annotation2384030
version: 1
top level: BBa_K1399013
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384030/range2384030/1
type: Range
persistent identity: range2384030
display id: range2384030
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 1069
end: 1088
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2384014
display id: annotation2384014
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384014/range2384014/1
type: Range
persistent identity: range2384014
display id: range2384014
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 89
end: 126
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384018
display id: annotation2384018
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384018/range2384018/1
type: Range
persistent identity: range2384018
display id: range2384018
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 173
end: 173
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2384017
display id: annotation2384017
version: 1
top level: BBa_K1399013
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384017/range2384017/1
type: Range
persistent identity: range2384017
display id: range2384017
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 166
end: 200
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2384023
display id: annotation2384023
version: 1
top level: BBa_K1399013
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384023/range2384023/1
type: Range
persistent identity: range2384023
display id: range2384023
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 902
end: 934
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2384021
display id: annotation2384021
version: 1
top level: BBa_K1399013
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2384021/range2384021/1
type: Range
persistent identity: range2384021
display id: range2384021
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 227
end: 229
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386313
display id: annotation2386313
version: 1
top level: BBa_K1399013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1

https://synbiohub.org/public/igem/BBa_K1399013/annotation2386313/range2386313/1
type: Range
persistent identity: range2386313
display id: range2386313
version: 1
direction: direction/forward
top level: BBa_K1399013
start: 941
end: 965
orientation: inline

BBa_K1465307
type: ComponentDefinition
persistent identity: BBa K1465307
display id: BBa_K1465307
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Isobutanol pathway with adhA from Lactococcus lactis under Ptac control
creator: iGEM-Team Bielefeld 2014
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1844_
m user id: 0
owner id: 20850
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ptac_alsS_ilvC_ilvD_kivD_adhA
mutable notes: bb
mutable provenance: bb
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K731500
component: BBa_K1465303
sequence annotation: BBa_K731500
sequence annotation: BBa_K1465303
sequence: https://synbiohub.org/public/igem/BBa_K1465307_sequence/1

BBa_K731500
type: Component
persistent identity: component2407338
display id: component2407338
version: 1
top level: BBa_K1465307
access: public
definition: https://synbiohub.org/public/igem/BBa_K731500/1

BBa_K1465303
type: Component
persistent identity: component2407341
display id: component2407341
version: 1
top level: BBa_K1465307
access: public
definition: https://synbiohub.org/public/igem/BBa_K1465303/1

BBa_K731500
type: SequenceAnnotation
persistent identity: annotation2407338
display id: annotation2407338
version: 1
top level: BBa_K1465307
location: https://synbiohub.org/public/igem/BBa_K1465307/annotation2407338/range2407338/1
component: BBa_K731500

https://synbiohub.org/public/igem/BBa_K1465307/annotation2407338/range2407338/1
type: Range
persistent identity: range2407338
display id: range2407338
version: 1
direction: direction/reverse
top level: BBa_K1465307
start: 1
end: 1239
orientation: inline

BBa_K1465303
type: SequenceAnnotation
persistent identity: annotation2407341
display id: annotation2407341
version: 1
top level: BBa_K1465307
location: https://synbiohub.org/public/igem/BBa_K1465307/annotation2407341/range2407341/1
component: BBa_K1465303

https://synbiohub.org/public/igem/BBa_K1465307/annotation2407341/range2407341/1
type: Range
persistent identity: range2407341
display id: range2407341
version: 1
direction: direction/reverse
top level: BBa_K1465307
start: 1248
end: 9028
orientation: inline

BBa_K1450004
type: ComponentDefinition
persistent identity: BBa K1450004
display id: BBa_K1450004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD_T7 RNA polymerase
creator: Joe Ho
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:28Z
discontinued: false
dominant: false
group u list: _1828_
m user id: 0
owner id: 9774
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a T7 RNA polymerase under an arabinose promoter
mutable notes: None
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1450001
component: BBa_B0015
component: BBa_I13453
component: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_K1450001
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1450004_sequence/1

BBa_K1450001
type: Component
persistent identity: component2411795
display id: component2411795
version: 1
top level: BBa_K1450004
access: public
definition: https://synbiohub.org/public/igem/BBa_K1450001/1

BBa_B0015
type: Component
persistent identity: component2411802
display id: component2411802
version: 1
top level: BBa_K1450004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I13453
type: Component
persistent identity: component2411792
display id: component2411792
version: 1
top level: BBa_K1450004
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_B0034
type: Component
persistent identity: component2411794
display id: component2411794
version: 1
top level: BBa_K1450004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2411802
display id: annotation2411802
version: 1
top level: BBa_K1450004
location: https://synbiohub.org/public/igem/BBa_K1450004/annotation2411802/range2411802/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1450004/annotation2411802/range2411802/1
type: Range
persistent identity: range2411802
display id: range2411802
version: 1
direction: direction/reverse
top level: BBa_K1450004
start: 2820
end: 2948
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2411794
display id: annotation2411794
version: 1
top level: BBa_K1450004
location: https://synbiohub.org/public/igem/BBa_K1450004/annotation2411794/range2411794/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1450004/annotation2411794/range2411794/1
type: Range
persistent identity: range2411794
display id: range2411794
version: 1
direction: direction/reverse
top level: BBa_K1450004
start: 139
end: 150
orientation: inline

BBa_K1450001
type: SequenceAnnotation
persistent identity: annotation2411795
display id: annotation2411795
version: 1
top level: BBa_K1450004
location: https://synbiohub.org/public/igem/BBa_K1450004/annotation2411795/range2411795/1
component: BBa_K1450001

https://synbiohub.org/public/igem/BBa_K1450004/annotation2411795/range2411795/1
type: Range
persistent identity: range2411795
display id: range2411795
version: 1
direction: direction/reverse
top level: BBa_K1450004
start: 157
end: 2811
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2411792
display id: annotation2411792
version: 1
top level: BBa_K1450004
location: https://synbiohub.org/public/igem/BBa_K1450004/annotation2411792/range2411792/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1450004/annotation2411792/range2411792/1
type: Range
persistent identity: range2411792
display id: range2411792
version: 1
direction: direction/reverse
top level: BBa_K1450004
start: 1
end: 130
orientation: inline

BBa_K1321333
type: ComponentDefinition
persistent identity: BBa K1321333
display id: BBa_K1321333
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pSB-AraC-pBAD
creator: Laura de Arroyo Garcia
created: 2014-10-07T11:00:00Z
modified: 2015-06-17T12:27:41Z
discontinued: false
dominant: false
group u list: _1696_
m user id: 4206
owner id: 21121
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This regulatory part is made up of the Arabinose-Inducible promoter, pBAD, and its transcriptional inhibitor/activator, AraC. AraC inhibits gene expression in the absence of Arabinose by binding to the operator units present within the AraC and pBAD promoter sequences, and producing a DNA loop that prevents the RNA polymerase and CAP from binding and initiating transcription. When Arabinose is supplied, this binds to the AraC dimer and releases it from the operator site found in the pBAD promoter, at the same time it enhances transcription by allowing binding to the enhancer sites upstream.
mutable provenance: This part was cloned out of existing templates (such as BBa_K325218, BBa_325219, all of which contain sfGFP downstream of the pBAD promoter)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraC
sequence annotation: PC
sequence annotation: pBAD
sequence: https://synbiohub.org/public/igem/BBa_K1321333_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation2429237
display id: annotation2429237
version: 1
top level: BBa_K1321333
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321333/annotation2429237/range2429237/1

https://synbiohub.org/public/igem/BBa_K1321333/annotation2429237/range2429237/1
type: Range
persistent identity: range2429237
display id: range2429237
version: 1
direction: direction/reverse
top level: BBa_K1321333
start: 1
end: 1041
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2429238
display id: annotation2429238
version: 1
top level: BBa_K1321333
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1321333/annotation2429238/range2429238/1

https://synbiohub.org/public/igem/BBa_K1321333/annotation2429238/range2429238/1
type: Range
persistent identity: range2429238
display id: range2429238
version: 1
direction: direction/reverse
top level: BBa_K1321333
start: 1042
end: 1115
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2429239
display id: annotation2429239
version: 1
top level: BBa_K1321333
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1321333/annotation2429239/range2429239/1

https://synbiohub.org/public/igem/BBa_K1321333/annotation2429239/range2429239/1
type: Range
persistent identity: range2429239
display id: range2429239
version: 1
direction: direction/forward
top level: BBa_K1321333
start: 1116
end: 1210
orientation: inline

BBa_K1399003
type: ComponentDefinition
persistent identity: BBa K1399003
display id: BBa_K1399003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP from Discosoma striata (coral) with DAS-ssrA degradation tag
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Mutant RFP from Discosoma striata (coral) (see part BBa_E1010) with added DAS-ssrA degradation tag (part BBa_M0052). The tag increases RFP turn-over rate, thus providing better temporal resolution of red fluorescence. In the same time, maximal fluorescence amplitudes will be lower as newly formed protein is degraded as soon as it is formed. The tag encodes peptide sequence AANDENYADAS and is recognized by ClpA and ClpX unfoldases and ClpX mediator SspB.[1] ClpA and ClpX then form a proteosome-like complex with ClpP protease and the protein is degraded.[1] The final three residues of the tag determines the strength of interaction with ClpX and thus the final protein degradation rate.[2] The DAS tag is reported to have low affinity to ClpX thus its mediated degradation very much depends on the concentration of SspB (ClpX mediator).[1] However, be aware that exact protein degradation rate is influenced by multiple other factors: ClpXP and ClpAP protease concentrations, protein stability, Km of binding to the protease, temperature [3]. References: [1] Flynn, J. M. et al. Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. U. S. A. 98, 10584???9 (2001). [2] Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240???6 (1998). [3] Purcell, O., Grierson, C. S., Bernardo, M. Di & Savery, N. J. Temperature dependence of ssrA-tag mediated protein degradation. J. Biol. Eng. 6, 10 (2012).
mutable notes: The tag was attached to RFP using PCR and MABEL (mutagenesis with blunt-end ligation), thus avoiding introduction of additonal residues and restriction site. Different parts of the tag are recognized by different proteins, for example, the final 3 residues (AAV in this case) are recognised by ClpX, whereas first 4 residues of the tag are required for efficient SspB binding.[1] Thus modifications of these critical residues alter the efficacy with what different proteases bind to it.
mutable provenance: RFP comes from part BBa_E1010, tag sequence was obtained from part BBa_M0052, but same tag was also used in paper by Andersen et al., (1998).[2]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: RFP
sequence annotation: DAS-ssrA tag
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_K1399003_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation2383906
display id: annotation2383906
version: 1
top level: BBa_K1399003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383906/range2383906/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383906/range2383906/1
type: Range
persistent identity: range2383906
display id: range2383906
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 709
end: 711
orientation: inline

RFP
type: SequenceAnnotation
persistent identity: annotation2383904
display id: annotation2383904
version: 1
top level: BBa_K1399003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383904/range2383904/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383904/range2383904/1
type: Range
persistent identity: range2383904
display id: range2383904
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 4
end: 675
orientation: inline

DAS-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383905
display id: annotation2383905
version: 1
top level: BBa_K1399003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383905/range2383905/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383905/range2383905/1
type: Range
persistent identity: range2383905
display id: range2383905
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 676
end: 708
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383907
display id: annotation2383907
version: 1
top level: BBa_K1399003
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383907/range2383907/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383907/range2383907/1
type: Range
persistent identity: range2383907
display id: range2383907
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 712
end: 714
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383903
display id: annotation2383903
version: 1
top level: BBa_K1399003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399003/annotation2383903/range2383903/1

https://synbiohub.org/public/igem/BBa_K1399003/annotation2383903/range2383903/1
type: Range
persistent identity: range2383903
display id: range2383903
version: 1
direction: direction/forward
top level: BBa_K1399003
start: 1
end: 3
orientation: inline

BBa_K1315006
type: ComponentDefinition
persistent identity: BBa K1315006
display id: BBa_K1315006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The manA promoter from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the promoter manA from Xcc.
mutable notes: The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: manA
sequence: https://synbiohub.org/public/igem/BBa_K1315006_sequence/1

manA
type: SequenceAnnotation
persistent identity: annotation2386904
display id: annotation2386904
version: 1
top level: BBa_K1315006
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1315006/annotation2386904/range2386904/1

https://synbiohub.org/public/igem/BBa_K1315006/annotation2386904/range2386904/1
type: Range
persistent identity: range2386904
display id: range2386904
version: 1
direction: direction/forward
top level: BBa_K1315006
start: 1
end: 250
orientation: inline

BBa_K1456002
type: ComponentDefinition
persistent identity: BBa K1456002
display id: BBa_K1456002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Glutathione Peroxidase-1 (GPX1)
creator: Mustafa Semih Elitok
created: 2014-07-20T11:00:00Z
modified: 2015-05-08T01:10:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1834_
m user id: 0
owner id: 9573
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This enzyme converts hydrogen peroxide into water Our cells will produce this enzyme to degrade oxygen radicals within the cells to prevent harmful effects.
mutable notes: None
mutable provenance: We clonned this part from human hepatocellular carcinoma cell genome cDNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1456002_sequence/1

BBa_K1333309
type: ComponentDefinition
persistent identity: BBa K1333309
display id: BBa_K1333309
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23119-FourU-RFP
creator: Runwen Yao
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15873
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is a combination of a constitutive promoter(BBa_J23119), RNA thermometer(BBa_K115002), and a RFP gene(BBa_E1010).
mutable provenance: The source of the part will be its components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23119
component: BBa_E1010
component: BBa_K115002
sequence annotation: BBa_J23119
sequence annotation: BBa_K115002
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K1333309_sequence/1

BBa_J23119
type: Component
persistent identity: component2385958
display id: component2385958
version: 1
top level: BBa_K1333309
access: public
definition: https://synbiohub.org/public/igem/BBa_J23119/1

BBa_E1010
type: Component
persistent identity: component2385966
display id: component2385966
version: 1
top level: BBa_K1333309
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_K115002
type: Component
persistent identity: component2385963
display id: component2385963
version: 1
top level: BBa_K1333309
access: public
definition: https://synbiohub.org/public/igem/BBa_K115002/1

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2385958
display id: annotation2385958
version: 1
top level: BBa_K1333309
location: https://synbiohub.org/public/igem/BBa_K1333309/annotation2385958/range2385958/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1333309/annotation2385958/range2385958/1
type: Range
persistent identity: range2385958
display id: range2385958
version: 1
direction: direction/reverse
top level: BBa_K1333309
start: 1
end: 35
orientation: inline

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2385963
display id: annotation2385963
version: 1
top level: BBa_K1333309
location: https://synbiohub.org/public/igem/BBa_K1333309/annotation2385963/range2385963/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K1333309/annotation2385963/range2385963/1
type: Range
persistent identity: range2385963
display id: range2385963
version: 1
direction: direction/reverse
top level: BBa_K1333309
start: 44
end: 95
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2385966
display id: annotation2385966
version: 1
top level: BBa_K1333309
location: https://synbiohub.org/public/igem/BBa_K1333309/annotation2385966/range2385966/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1333309/annotation2385966/range2385966/1
type: Range
persistent identity: range2385966
display id: range2385966
version: 1
direction: direction/reverse
top level: BBa_K1333309
start: 102
end: 807
orientation: inline

BBa_K1497008
type: ComponentDefinition
persistent identity: BBa K1497008
display id: BBa_K1497008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hokD (relF)
creator: Sascha Hein, Daniel Sachs, Christian Sator
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: d
mutable notes: d
mutable provenance: d
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497008_sequence/1

BBa_K1489002
type: ComponentDefinition
persistent identity: BBa K1489002
display id: BBa_K1489002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lpp-OmpA-Linker (pSB1C3)
creator: Iowis Zhu
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:44Z
discontinued: false
dominant: false
group u list: _1869_
m user id: 0
owner id: 20382
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Outer membrane protein A (OmpA) is a common outer membrane protein found in bacteria that serves various purposes. For our purposes, the beta-barrel transmembrane domain can be utilized to anchor otherwise soluble proteins in the outer cell membrane. Lpp serves to direct the fusion protein, while a unstructured linker (GGGSGGGS) serves to separate OmpA from its cargo in order to avoid interactions between the two proteins. The expressed protein is structurally identical to BBa_K103006. This version of the biobrick has been moved into the new backbone, pSB1C3, which contains the gene for chloramphenicol resistance. This resistance gene contains an internal SacI site, making the SacI site at the end of the gene almost unusable for RE cloning. BBa_K1489003 mutagenizes this SacI site into a KasI in order to avoid this issue.
mutable notes: SacI site is kept in this version of Lpp-OmpA-Linker, is removed in BBa_K1489003.
mutable provenance: Part sequence is from BBa_K103006, which originally comes from the E. coli genome.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SacI site
sequence annotation: Lpp-OmpA
sequence annotation: Linker
sequence annotation: NdeI site
sequence annotation: ATG start
sequence: https://synbiohub.org/public/igem/BBa_K1489002_sequence/1

SacI site
type: SequenceAnnotation
persistent identity: annotation2397210
display id: annotation2397210
version: 1
top level: BBa_K1489002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1489002/annotation2397210/range2397210/1

https://synbiohub.org/public/igem/BBa_K1489002/annotation2397210/range2397210/1
type: Range
persistent identity: range2397210
display id: range2397210
version: 1
direction: direction/forward
top level: BBa_K1489002
start: 459
end: 464
orientation: inline

Lpp-OmpA
type: SequenceAnnotation
persistent identity: annotation2397212
display id: annotation2397212
version: 1
top level: BBa_K1489002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1489002/annotation2397212/range2397212/1

https://synbiohub.org/public/igem/BBa_K1489002/annotation2397212/range2397212/1
type: Range
persistent identity: range2397212
display id: range2397212
version: 1
direction: direction/forward
top level: BBa_K1489002
start: 4
end: 438
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2397211
display id: annotation2397211
version: 1
top level: BBa_K1489002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1489002/annotation2397211/range2397211/1

https://synbiohub.org/public/igem/BBa_K1489002/annotation2397211/range2397211/1
type: Range
persistent identity: range2397211
display id: range2397211
version: 1
direction: direction/forward
top level: BBa_K1489002
start: 439
end: 464
orientation: inline

NdeI site
type: SequenceAnnotation
persistent identity: annotation2397209
display id: annotation2397209
version: 1
top level: BBa_K1489002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1489002/annotation2397209/range2397209/1

https://synbiohub.org/public/igem/BBa_K1489002/annotation2397209/range2397209/1
type: Range
persistent identity: range2397209
display id: range2397209
version: 1
direction: direction/forward
top level: BBa_K1489002
start: 1
end: 6
orientation: inline

ATG start
type: SequenceAnnotation
persistent identity: annotation2397208
display id: annotation2397208
version: 1
top level: BBa_K1489002
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1489002/annotation2397208/range2397208/1

https://synbiohub.org/public/igem/BBa_K1489002/annotation2397208/range2397208/1
type: Range
persistent identity: range2397208
display id: range2397208
version: 1
direction: direction/forward
top level: BBa_K1489002
start: 4
end: 6
orientation: inline

BBa_K1330005
type: ComponentDefinition
persistent identity: BBa K1330005
display id: BBa_K1330005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23116
creator: Kristian Jensen
created: 2014-09-29T11:00:00Z
modified: 2015-05-08T01:09:55Z
discontinued: false
dominant: false
group u list: _1705_
m user id: 0
owner id: 20126
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: t
mutable notes: t
mutable provenance: t
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1330005_sequence/1

BBa_K1321200
type: ComponentDefinition
persistent identity: BBa K1321200
display id: BBa_K1321200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J23101+B0034+VHb (Vitreoscilla haemoglobin)
creator: Xenia Spencer-Milnes
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:52Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1696_
m user id: 0
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: J23101+RBS+VhB. TBC
mutable notes: J23101+RBS+VhB. TBC
mutable provenance: J23101+RBS+VhB. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Stop
sequence annotation: B0034
sequence annotation: Start
sequence annotation: VHb
sequence annotation: J23101 constitutive
sequence: https://synbiohub.org/public/igem/BBa_K1321200_sequence/1

Stop
type: SequenceAnnotation
persistent identity: annotation2422497
display id: annotation2422497
version: 1
top level: BBa_K1321200
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1321200/annotation2422497/range2422497/1

https://synbiohub.org/public/igem/BBa_K1321200/annotation2422497/range2422497/1
type: Range
persistent identity: range2422497
display id: range2422497
version: 1
direction: direction/forward
top level: BBa_K1321200
start: 502
end: 504
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2422493
display id: annotation2422493
version: 1
top level: BBa_K1321200
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1321200/annotation2422493/range2422493/1

https://synbiohub.org/public/igem/BBa_K1321200/annotation2422493/range2422493/1
type: Range
persistent identity: range2422493
display id: range2422493
version: 1
direction: direction/forward
top level: BBa_K1321200
start: 44
end: 55
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2422496
display id: annotation2422496
version: 1
top level: BBa_K1321200
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1321200/annotation2422496/range2422496/1

https://synbiohub.org/public/igem/BBa_K1321200/annotation2422496/range2422496/1
type: Range
persistent identity: range2422496
display id: range2422496
version: 1
direction: direction/forward
top level: BBa_K1321200
start: 64
end: 66
orientation: inline

VHb
type: SequenceAnnotation
persistent identity: annotation2422498
display id: annotation2422498
version: 1
top level: BBa_K1321200
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321200/annotation2422498/range2422498/1

https://synbiohub.org/public/igem/BBa_K1321200/annotation2422498/range2422498/1
type: Range
persistent identity: range2422498
display id: range2422498
version: 1
direction: direction/forward
top level: BBa_K1321200
start: 64
end: 504
orientation: inline

J23101 constitutive
type: SequenceAnnotation
persistent identity: annotation2422494
display id: annotation2422494
version: 1
top level: BBa_K1321200
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1321200/annotation2422494/range2422494/1

https://synbiohub.org/public/igem/BBa_K1321200/annotation2422494/range2422494/1
type: Range
persistent identity: range2422494
display id: range2422494
version: 1
direction: direction/forward
top level: BBa_K1321200
start: 1
end: 35
orientation: inline

BBa_K1362051
type: ComponentDefinition
persistent identity: BBa K1362051
display id: BBa_K1362051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intein protease with arabinose inducible regulatory promoter/ repressor araC-Pbad-T7RBS-His-SspDnaBC
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22911
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362050
component: BBa_K808000
sequence annotation: BBa_K1362050
sequence annotation: BBa_K808000
sequence: https://synbiohub.org/public/igem/BBa_K1362051_sequence/1

BBa_K1362050
type: Component
persistent identity: component2409673
display id: component2409673
version: 1
top level: BBa_K1362051
access: public
definition: BBa_K1362050

BBa_K808000
type: Component
persistent identity: component2409661
display id: component2409661
version: 1
top level: BBa_K1362051
access: public
definition: https://synbiohub.org/public/igem/BBa_K808000/1

BBa_K1362050
type: SequenceAnnotation
persistent identity: annotation2409673
display id: annotation2409673
version: 1
top level: BBa_K1362051
location: https://synbiohub.org/public/igem/BBa_K1362051/annotation2409673/range2409673/1
component: BBa_K1362050

https://synbiohub.org/public/igem/BBa_K1362051/annotation2409673/range2409673/1
type: Range
persistent identity: range2409673
display id: range2409673
version: 1
direction: direction/reverse
top level: BBa_K1362051
start: 1218
end: 1717
orientation: inline

BBa_K808000
type: SequenceAnnotation
persistent identity: annotation2409661
display id: annotation2409661
version: 1
top level: BBa_K1362051
location: https://synbiohub.org/public/igem/BBa_K1362051/annotation2409661/range2409661/1
component: BBa_K808000

https://synbiohub.org/public/igem/BBa_K1362051/annotation2409661/range2409661/1
type: Range
persistent identity: range2409661
display id: range2409661
version: 1
direction: direction/reverse
top level: BBa_K1362051
start: 1
end: 1209
orientation: inline

BBa_K1315003
type: ComponentDefinition
persistent identity: BBa K1315003
display id: BBa_K1315003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: The rpfG gene from Xanthomonas campestris
creator: Dimitrios Michailidis
created: 2014-09-24T11:00:00Z
modified: 2015-05-08T01:09:48Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the coding sequence for the RpfG(regulation of pathogenicity factor G) protein. It is the regulator in the two-component signal transduction system of Xanthomonas campestris pv. campestris that is activated by the sensor kinase RpfC(Bba_K1315002) in the presence of DSF(diffusible signal factor).
mutable notes: The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.
mutable provenance: The source of this part is Xanthomonas campestris pv. campestris(Xcc) genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: RpfG
sequence: https://synbiohub.org/public/igem/BBa_K1315003_sequence/1

RpfG
type: SequenceAnnotation
persistent identity: annotation2386405
display id: annotation2386405
version: 1
top level: BBa_K1315003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1315003/annotation2386405/range2386405/1

https://synbiohub.org/public/igem/BBa_K1315003/annotation2386405/range2386405/1
type: Range
persistent identity: range2386405
display id: range2386405
version: 1
direction: direction/forward
top level: BBa_K1315003
start: 1
end: 1140
orientation: inline

BBa_K1493520
type: ComponentDefinition
persistent identity: BBa K1493520
display id: BBa_K1493520
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pRHA+LacI
creator: Michiel Herpers
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_P0412
component: BBa_K914003
sequence annotation: BBa_K914003
sequence annotation: BBa_P0412
sequence: https://synbiohub.org/public/igem/BBa_K1493520_sequence/1

BBa_P0412
type: Component
persistent identity: component2396350
display id: component2396350
version: 1
top level: BBa_K1493520
access: public
definition: https://synbiohub.org/public/igem/BBa_P0412/1

BBa_K914003
type: Component
persistent identity: component2396337
display id: component2396337
version: 1
top level: BBa_K1493520
access: public
definition: https://synbiohub.org/public/igem/BBa_K914003/1

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2396337
display id: annotation2396337
version: 1
top level: BBa_K1493520
location: https://synbiohub.org/public/igem/BBa_K1493520/annotation2396337/range2396337/1
component: BBa_K914003

https://synbiohub.org/public/igem/BBa_K1493520/annotation2396337/range2396337/1
type: Range
persistent identity: range2396337
display id: range2396337
version: 1
direction: direction/reverse
top level: BBa_K1493520
start: 1
end: 122
orientation: inline

BBa_P0412
type: SequenceAnnotation
persistent identity: annotation2396350
display id: annotation2396350
version: 1
top level: BBa_K1493520
location: https://synbiohub.org/public/igem/BBa_K1493520/annotation2396350/range2396350/1
component: BBa_P0412

https://synbiohub.org/public/igem/BBa_K1493520/annotation2396350/range2396350/1
type: Range
persistent identity: range2396350
display id: range2396350
version: 1
direction: direction/reverse
top level: BBa_K1493520
start: 131
end: 1438
orientation: inline

BBa_K1393001
type: ComponentDefinition
persistent identity: BBa K1393001
display id: BBa_K1393001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OprF(Ala196)+CBP
creator: Jiajun Tan
created: 2014-10-01T11:00:00Z
modified: 2015-07-22T02:48:08Z
discontinued: false
dominant: false
group u list: _1770_
m user id: 4206
owner id: 20256
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The OprF is a major outer membrane protein of Pseudomonas aeruginosa. This protein functions as a nonspecific porin to allow the passage of small hydrophilic molecules, plays a structural role in maintaining cell shape and outer membrane integrity, and is required for growth under low osmolality. The structure of OprF has been proposed to consist of three domains, the N-terminal forming h-barrel structure, a loop or hinge region, and the C-terminal associated with peptidoglycan. Based on the predicted secondary structure and information found in the literature, we chose Ala196 as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.
mutable notes: Based on the predicted secondary structure and information found in the literature, we chose Val(188) as potential fusion sites for displaying CBP. CBP is the abbreviation of copper binding peptide made up of seven amino acid.
mutable provenance: The OprF gene is from Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: oprF to Ala196
sequence annotation: CBP
sequence: https://synbiohub.org/public/igem/BBa_K1393001_sequence/1

oprF to Ala196
type: SequenceAnnotation
persistent identity: annotation2391711
display id: annotation2391711
version: 1
top level: BBa_K1393001
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1393001/annotation2391711/range2391711/1

https://synbiohub.org/public/igem/BBa_K1393001/annotation2391711/range2391711/1
type: Range
persistent identity: range2391711
display id: range2391711
version: 1
direction: direction/forward
top level: BBa_K1393001
start: 1
end: 588
orientation: inline

CBP
type: SequenceAnnotation
persistent identity: annotation2391717
display id: annotation2391717
version: 1
top level: BBa_K1393001
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1393001/annotation2391717/range2391717/1

https://synbiohub.org/public/igem/BBa_K1393001/annotation2391717/range2391717/1
type: Range
persistent identity: range2391717
display id: range2391717
version: 1
direction: direction/forward
top level: BBa_K1393001
start: 589
end: 609
orientation: inline

BBa_K1343014
type: ComponentDefinition
persistent identity: BBa K1343014
display id: BBa_K1343014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux-green renilla luciferase
creator: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
created: 2014-09-26T11:00:00Z
modified: 2015-05-08T01:09:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1718_
m user id: 0
owner id: 20512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: E.coli optimized green renilla luciferase under LuxR-AHL regulated promoter
mutable notes: To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other
mutable provenance: Placeholder
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_B0030: Strong RBS
sequence annotation: barcode 5
sequence annotation: BBa_R0062: P lux
sequence annotation: T7 terminator
sequence annotation: barcode 3
sequence annotation: barcode 6
sequence annotation: barcode 2
sequence annotation: green renilla luciferase
sequence annotation: BBa_B0010:T1 (terminator) from E. coli
sequence annotation: barcode 1
sequence annotation: barcode 4
sequence: https://synbiohub.org/public/igem/BBa_K1343014_sequence/1

BBa_B0030: Strong RBS
type: SequenceAnnotation
persistent identity: annotation2428051
display id: annotation2428051
version: 1
top level: BBa_K1343014
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428051/range2428051/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428051/range2428051/1
type: Range
persistent identity: range2428051
display id: range2428051
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 122
end: 136
orientation: inline

barcode 5
type: SequenceAnnotation
persistent identity: annotation2428055
display id: annotation2428055
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428055/range2428055/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428055/range2428055/1
type: Range
persistent identity: range2428055
display id: range2428055
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1123
end: 1144
orientation: inline

BBa_R0062: P lux
type: SequenceAnnotation
persistent identity: annotation2428047
display id: annotation2428047
version: 1
top level: BBa_K1343014
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428047/range2428047/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428047/range2428047/1
type: Range
persistent identity: range2428047
display id: range2428047
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1
end: 55
orientation: inline

T7 terminator
type: SequenceAnnotation
persistent identity: annotation2428057
display id: annotation2428057
version: 1
top level: BBa_K1343014
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428057/range2428057/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428057/range2428057/1
type: Range
persistent identity: range2428057
display id: range2428057
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1225
end: 1271
orientation: inline

barcode 3
type: SequenceAnnotation
persistent identity: annotation2428050
display id: annotation2428050
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428050/range2428050/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428050/range2428050/1
type: Range
persistent identity: range2428050
display id: range2428050
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 100
end: 121
orientation: inline

barcode 6
type: SequenceAnnotation
persistent identity: annotation2428048
display id: annotation2428048
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428048/range2428048/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428048/range2428048/1
type: Range
persistent identity: range2428048
display id: range2428048
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 56
end: 77
orientation: inline

barcode 2
type: SequenceAnnotation
persistent identity: annotation2428054
display id: annotation2428054
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428054/range2428054/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428054/range2428054/1
type: Range
persistent identity: range2428054
display id: range2428054
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1101
end: 1122
orientation: inline

green renilla luciferase
type: SequenceAnnotation
persistent identity: annotation2428052
display id: annotation2428052
version: 1
top level: BBa_K1343014
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428052/range2428052/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428052/range2428052/1
type: Range
persistent identity: range2428052
display id: range2428052
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 143
end: 1078
orientation: inline

BBa_B0010:T1 (terminator) from E. coli
type: SequenceAnnotation
persistent identity: annotation2428056
display id: annotation2428056
version: 1
top level: BBa_K1343014
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428056/range2428056/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428056/range2428056/1
type: Range
persistent identity: range2428056
display id: range2428056
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1145
end: 1224
orientation: inline

barcode 1
type: SequenceAnnotation
persistent identity: annotation2428049
display id: annotation2428049
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428049/range2428049/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428049/range2428049/1
type: Range
persistent identity: range2428049
display id: range2428049
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 78
end: 99
orientation: inline

barcode 4
type: SequenceAnnotation
persistent identity: annotation2428053
display id: annotation2428053
version: 1
top level: BBa_K1343014
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_K1343014/annotation2428053/range2428053/1

https://synbiohub.org/public/igem/BBa_K1343014/annotation2428053/range2428053/1
type: Range
persistent identity: range2428053
display id: range2428053
version: 1
direction: direction/forward
top level: BBa_K1343014
start: 1079
end: 1100
orientation: inline

BBa_K1404008
type: ComponentDefinition
persistent identity: BBa K1404008
display id: BBa_K1404008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His*2, double His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: Beta sheet
sequence annotation: CsgD signal
sequence annotation: BBa_K342000
sequence annotation: Beta sheet2
sequence annotation: His tag
sequence annotation: linker
sequence annotation: SEC signal
sequence annotation: GCT into GCC
sequence annotation: CsgA-His2
sequence annotation: Linker
sequence annotation: Beta sheet5
sequence annotation: RBS Paris 2009
sequence annotation: Beta sheet4
sequence: https://synbiohub.org/public/igem/BBa_K1404008_sequence/1

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426514
display id: annotation2426514
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426514/range2426514/1
type: Range
persistent identity: range2426514
display id: range2426514
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 353
end: 421
orientation: inline

CsgD signal
type: SequenceAnnotation
persistent identity: annotation2426511
display id: annotation2426511
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426511/range2426511/1
type: Range
persistent identity: range2426511
display id: range2426511
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 152
end: 217
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426507
display id: annotation2426507
version: 1
top level: BBa_K1404008
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426507/range2426507/1
type: Range
persistent identity: range2426507
display id: range2426507
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 1
end: 70
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426513
display id: annotation2426513
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426513/range2426513/1
type: Range
persistent identity: range2426513
display id: range2426513
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 287
end: 352
orientation: inline

Beta sheet
type: SequenceAnnotation
persistent identity: annotation2426512
display id: annotation2426512
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426512/range2426512/1
type: Range
persistent identity: range2426512
display id: range2426512
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 218
end: 286
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426519
display id: annotation2426519
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426519/range2426519/1
type: Range
persistent identity: range2426519
display id: range2426519
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 557
end: 574
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2426520
display id: annotation2426520
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426520/range2426520/1
type: Range
persistent identity: range2426520
display id: range2426520
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 575
end: 586
orientation: inline

SEC signal
type: SequenceAnnotation
persistent identity: annotation2426509
display id: annotation2426509
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426509/range2426509/1
type: Range
persistent identity: range2426509
display id: range2426509
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 151
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426516
display id: annotation2426516
version: 1
top level: BBa_K1404008
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426516/range2426516/1
type: Range
persistent identity: range2426516
display id: range2426516
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 461
end: 463
orientation: inline

CsgA-His2
type: SequenceAnnotation
persistent identity: annotation2426510
display id: annotation2426510
version: 1
top level: BBa_K1404008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426510/range2426510/1
type: Range
persistent identity: range2426510
display id: range2426510
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 92
end: 607
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426518
display id: annotation2426518
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426518/range2426518/1
type: Range
persistent identity: range2426518
display id: range2426518
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 545
end: 556
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426521
display id: annotation2426521
version: 1
top level: BBa_K1404008
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426521/range2426521/1
type: Range
persistent identity: range2426521
display id: range2426521
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 587
end: 604
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426517
display id: annotation2426517
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426517/range2426517/1
type: Range
persistent identity: range2426517
display id: range2426517
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 488
end: 544
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426508
display id: annotation2426508
version: 1
top level: BBa_K1404008
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426508/range2426508/1
type: Range
persistent identity: range2426508
display id: range2426508
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 79
end: 91
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426515
display id: annotation2426515
version: 1
top level: BBa_K1404008
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1

https://synbiohub.org/public/igem/BBa_K1404008/annotation2426515/range2426515/1
type: Range
persistent identity: range2426515
display id: range2426515
version: 1
direction: direction/forward
top level: BBa_K1404008
start: 422
end: 487
orientation: inline

BBa_K1362059
type: ComponentDefinition
persistent identity: BBa K1362059
display id: BBa_K1362059
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PKI-NES as RFC[105] N-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
component: BBa_K1362425
component: BBa_J63007
component: BBa_K1362423
component: BBa_K1362440
component: BBa_K1362444
component: BBa_K1362415
sequence annotation: BBa_K1362415
sequence annotation: BBa_K1362423
sequence annotation: BBa_J63007
sequence annotation: BBa_K1362425
sequence annotation: BBa_K1362444
sequence annotation: BBa_K1362440
sequence: https://synbiohub.org/public/igem/BBa_K1362059_sequence/1

BBa_K1362425
type: Component
persistent identity: component2409741
display id: component2409741
version: 1
top level: BBa_K1362059
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_J63007
type: Component
persistent identity: component2409743
display id: component2409743
version: 1
top level: BBa_K1362059
access: public
definition: https://synbiohub.org/public/igem/BBa_J63007/1

BBa_K1362423
type: Component
persistent identity: component2409746
display id: component2409746
version: 1
top level: BBa_K1362059
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362440
type: Component
persistent identity: component2409742
display id: component2409742
version: 1
top level: BBa_K1362059
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362440/1

BBa_K1362444
type: Component
persistent identity: component2409744
display id: component2409744
version: 1
top level: BBa_K1362059
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362444/1

BBa_K1362415
type: Component
persistent identity: component2409745
display id: component2409745
version: 1
top level: BBa_K1362059
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409745
display id: annotation2409745
version: 1
top level: BBa_K1362059
location: https://synbiohub.org/public/igem/BBa_K1362059/annotation2409745/range2409745/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362059/annotation2409745/range2409745/1
type: Range
persistent identity: range2409745
display id: range2409745
version: 1
direction: direction/reverse
top level: BBa_K1362059
start: 59
end: 62
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409746
display id: annotation2409746
version: 1
top level: BBa_K1362059
location: https://synbiohub.org/public/igem/BBa_K1362059/annotation2409746/range2409746/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362059/annotation2409746/range2409746/1
type: Range
persistent identity: range2409746
display id: range2409746
version: 1
direction: direction/reverse
top level: BBa_K1362059
start: 63
end: 69
orientation: inline

BBa_J63007
type: SequenceAnnotation
persistent identity: annotation2409743
display id: annotation2409743
version: 1
top level: BBa_K1362059
location: https://synbiohub.org/public/igem/BBa_K1362059/annotation2409743/range2409743/1
component: BBa_J63007

https://synbiohub.org/public/igem/BBa_K1362059/annotation2409743/range2409743/1
type: Range
persistent identity: range2409743
display id: range2409743
version: 1
direction: direction/reverse
top level: BBa_K1362059
start: 11
end: 40
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409741
display id: annotation2409741
version: 1
top level: BBa_K1362059
location: https://synbiohub.org/public/igem/BBa_K1362059/annotation2409741/range2409741/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362059/annotation2409741/range2409741/1
type: Range
persistent identity: range2409741
display id: range2409741
version: 1
direction: direction/reverse
top level: BBa_K1362059
start: 1
end: 6
orientation: inline

BBa_K1362444
type: SequenceAnnotation
persistent identity: annotation2409744
display id: annotation2409744
version: 1
top level: BBa_K1362059
location: https://synbiohub.org/public/igem/BBa_K1362059/annotation2409744/range2409744/1
component: BBa_K1362444

https://synbiohub.org/public/igem/BBa_K1362059/annotation2409744/range2409744/1
type: Range
persistent identity: range2409744
display id: range2409744
version: 1
direction: direction/reverse
top level: BBa_K1362059
start: 41
end: 58
orientation: inline

BBa_K1362440
type: SequenceAnnotation
persistent identity: annotation2409742
display id: annotation2409742
version: 1
top level: BBa_K1362059
location: https://synbiohub.org/public/igem/BBa_K1362059/annotation2409742/range2409742/1
component: BBa_K1362440

https://synbiohub.org/public/igem/BBa_K1362059/annotation2409742/range2409742/1
type: Range
persistent identity: range2409742
display id: range2409742
version: 1
direction: direction/reverse
top level: BBa_K1362059
start: 7
end: 10
orientation: inline

BBa_K1401601
type: ComponentDefinition
persistent identity: BBa K1401601
display id: BBa_K1401601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: C0080_CI, MoClo of BBa_C0080
creator: Yash Agarwal
created: 2014-07-20T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1779_
m user id: 0
owner id: 23227
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a MoClo formatted C0080_CI araC gene. This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part.
mutable notes: The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; I = TCTA.
mutable provenance: Will enter later.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1401601_sequence/1

BBa_K1333107
type: ComponentDefinition
persistent identity: BBa K1333107
display id: BBa_K1333107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: umuc
creator: Zhang Fangyingnan
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 18999
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: umuc coding sequence
mutable notes: original E.coli gene sequence
mutable provenance: from E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K1333107_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2429478
display id: annotation2429478
version: 1
top level: BBa_K1333107
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1333107/annotation2429478/range2429478/1

https://synbiohub.org/public/igem/BBa_K1333107/annotation2429478/range2429478/1
type: Range
persistent identity: range2429478
display id: range2429478
version: 1
direction: direction/forward
top level: BBa_K1333107
start: 1
end: 1269
orientation: inline

BBa_K1323002
type: ComponentDefinition
persistent identity: BBa K1323002
display id: BBa_K1323002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas9: Expression cassette under a xylose inducible promoter
creator: Julia Manalil
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:09:53Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1698_
m user id: 0
owner id: 18368
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TBA
mutable notes: TBA
mutable provenance: TBA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: xylose inducible promoter
sequence annotation: sodA RBS
sequence annotation: transcriptional terminator
sequence annotation: dCas9
sequence: https://synbiohub.org/public/igem/BBa_K1323002_sequence/1

xylose inducible promoter
type: SequenceAnnotation
persistent identity: annotation2428742
display id: annotation2428742
version: 1
top level: BBa_K1323002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1323002/annotation2428742/range2428742/1

https://synbiohub.org/public/igem/BBa_K1323002/annotation2428742/range2428742/1
type: Range
persistent identity: range2428742
display id: range2428742
version: 1
direction: direction/forward
top level: BBa_K1323002
start: 1
end: 1484
orientation: inline

sodA RBS
type: SequenceAnnotation
persistent identity: annotation2428743
display id: annotation2428743
version: 1
top level: BBa_K1323002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1323002/annotation2428743/range2428743/1

https://synbiohub.org/public/igem/BBa_K1323002/annotation2428743/range2428743/1
type: Range
persistent identity: range2428743
display id: range2428743
version: 1
direction: direction/forward
top level: BBa_K1323002
start: 1493
end: 1510
orientation: inline

transcriptional terminator
type: SequenceAnnotation
persistent identity: annotation2428748
display id: annotation2428748
version: 1
top level: BBa_K1323002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1323002/annotation2428748/range2428748/1

https://synbiohub.org/public/igem/BBa_K1323002/annotation2428748/range2428748/1
type: Range
persistent identity: range2428748
display id: range2428748
version: 1
direction: direction/forward
top level: BBa_K1323002
start: 5686
end: 5732
orientation: inline

dCas9
type: SequenceAnnotation
persistent identity: annotation2428744
display id: annotation2428744
version: 1
top level: BBa_K1323002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1323002/annotation2428744/range2428744/1

https://synbiohub.org/public/igem/BBa_K1323002/annotation2428744/range2428744/1
type: Range
persistent identity: range2428744
display id: range2428744
version: 1
direction: direction/forward
top level: BBa_K1323002
start: 1517
end: 5623
orientation: inline

BBa_K1499400
type: ComponentDefinition
persistent identity: BBa K1499400
display id: BBa_K1499400
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Chitin-binding wasp protein 1 (PdomMRNAr1.2-03231.1)
creator: Ian Hull
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1879_
m user id: 0
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: todo
mutable notes: todo
mutable provenance: todo
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: chitin-binding protein 1
sequence annotation: TAA stop codon
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_K1499400_sequence/1

chitin-binding protein 1
type: SequenceAnnotation
persistent identity: annotation2413313
display id: annotation2413313
version: 1
top level: BBa_K1499400
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1499400/annotation2413313/range2413313/1

https://synbiohub.org/public/igem/BBa_K1499400/annotation2413313/range2413313/1
type: Range
persistent identity: range2413313
display id: range2413313
version: 1
direction: direction/forward
top level: BBa_K1499400
start: 9
end: 422
orientation: inline

TAA stop codon
type: SequenceAnnotation
persistent identity: annotation2413314
display id: annotation2413314
version: 1
top level: BBa_K1499400
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1499400/annotation2413314/range2413314/1

https://synbiohub.org/public/igem/BBa_K1499400/annotation2413314/range2413314/1
type: Range
persistent identity: range2413314
display id: range2413314
version: 1
direction: direction/forward
top level: BBa_K1499400
start: 423
end: 425
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation2413315
display id: annotation2413315
version: 1
top level: BBa_K1499400
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499400/annotation2413315/range2413315/1

https://synbiohub.org/public/igem/BBa_K1499400/annotation2413315/range2413315/1
type: Range
persistent identity: range2413315
display id: range2413315
version: 1
direction: direction/forward
top level: BBa_K1499400
start: 9
end: 11
orientation: inline

CircNpu
type: ComponentDefinition
persistent identity: BBa K1362000
display id: BBa_K1362000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NpuDnaE intein RFC[105] circularization construct
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Buescher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schaefer, Carolin Schmelas, Silvan Schmitz, Max Waldhauer
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ???
mutable notes: ???
mutable provenance: ???
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362424
component: BBa_K1362401
component: BBa_K1362999
component: BBa_K1362400
component: BBa_K1362419
component: BBa_K1362423
component: BBa_K1362421
component: BBa_G0000
component: BBa_K1362090
component: BBa_K1362505
component: BBa_K1362415
component: BBa_K1362414
sequence annotation: BBa_K1362414
sequence annotation: BBa_K1362999
sequence annotation: BBa_G0000
sequence annotation: BBa_K1362424
sequence annotation: BBa_K1362421
sequence annotation: BBa_K1362419
sequence annotation: BBa_K1362505
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362400
sequence annotation: BBa_K1362090
sequence annotation: BBa_K1362401
sequence annotation: BBa_K1362415
sequence: https://synbiohub.org/public/igem/BBa_K1362000_sequence/1

BBa_K1362424
type: Component
persistent identity: component2427479
display id: component2427479
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362424/1

BBa_K1362401
type: Component
persistent identity: component2427474
display id: component2427474
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362401/1

BBa_K1362999
type: Component
persistent identity: component2427487
display id: component2427487
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362999/1

BBa_K1362400
type: Component
persistent identity: component2427481
display id: component2427481
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362400/1

BBa_K1362419
type: Component
persistent identity: component2427475
display id: component2427475
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362419/1

BBa_K1362423
type: Component
persistent identity: component2427476
display id: component2427476
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_K1362421
type: Component
persistent identity: component2427483
display id: component2427483
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362421/1

BBa_G0000
type: Component
persistent identity: component2427472
display id: component2427472
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_G0000/1

BBa_K1362090
type: Component
persistent identity: component2427471
display id: component2427471
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362090/1

BBa_K1362505
type: Component
persistent identity: component2427478
display id: component2427478
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362505/1

BBa_K1362415
type: Component
persistent identity: component2427480
display id: component2427480
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362414
type: Component
persistent identity: component2427473
display id: component2427473
version: 1
top level: CircNpu
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362414/1

BBa_K1362414
type: SequenceAnnotation
persistent identity: annotation2427473
display id: annotation2427473
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427473/range2427473/1
component: BBa_K1362414

https://synbiohub.org/public/igem/BBa_K1362000/annotation2427473/range2427473/1
type: Range
persistent identity: range2427473
display id: range2427473
version: 1
direction: direction/reverse
top level: CircNpu
start: 36
end: 38
orientation: inline

BBa_K1362999
type: SequenceAnnotation
persistent identity: annotation2427487
display id: annotation2427487
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427487/range2427487/1
component: BBa_K1362999

https://synbiohub.org/public/igem/BBa_K1362000/annotation2427487/range2427487/1
type: Range
persistent identity: range2427487
display id: range2427487
version: 1
direction: direction/reverse
top level: CircNpu
start: 1537
end: 1562
orientation: inline

BBa_G0000
type: SequenceAnnotation
persistent identity: annotation2427472
display id: annotation2427472
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427472/range2427472/1
component: BBa_G0000

https://synbiohub.org/public/igem/BBa_K1362000/annotation2427472/range2427472/1
type: Range
persistent identity: range2427472
display id: range2427472
version: 1
direction: direction/reverse
top level: CircNpu
start: 30
end: 35
orientation: inline

BBa_K1362424
type: SequenceAnnotation
persistent identity: annotation2427479
display id: annotation2427479
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427479/range2427479/1
component: BBa_K1362424

https://synbiohub.org/public/igem/BBa_K1362000/annotation2427479/range2427479/1
type: Range
persistent identity: range2427479
display id: range2427479
version: 1
direction: direction/reverse
top level: CircNpu
start: 1220
end: 1226
orientation: inline

BBa_K1362421
type: SequenceAnnotation
persistent identity: annotation2427483
display id: annotation2427483
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427483/range2427483/1
component: BBa_K1362421

https://synbiohub.org/public/igem/BBa_K1362000/annotation2427483/range2427483/1
type: Range
persistent identity: range2427483
display id: range2427483
version: 1
direction: direction/reverse
top level: CircNpu
start: 1533
end: 1536
orientation: inline

BBa_K1362419
type: SequenceAnnotation
persistent identity: annotation2427475
display id: annotation2427475
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427475/range2427475/1
component: BBa_K1362419

https://synbiohub.org/public/igem/BBa_K1362000/annotation2427475/range2427475/1
type: Range
persistent identity: range2427475
display id: range2427475
version: 1
direction: direction/reverse
top level: CircNpu
start: 140
end: 143
orientation: inline

BBa_K1362505
type: SequenceAnnotation
persistent identity: annotation2427478
display id: annotation2427478
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427478/range2427478/1
component: BBa_K1362505

https://synbiohub.org/public/igem/BBa_K1362000/annotation2427478/range2427478/1
type: Range
persistent identity: range2427478
display id: range2427478
version: 1
direction: direction/reverse
top level: CircNpu
start: 151
end: 1219
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2427476
display id: annotation2427476
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427476/range2427476/1
component: BBa_K1362423

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type: Range
persistent identity: range2427476
display id: range2427476
version: 1
direction: direction/reverse
top level: CircNpu
start: 144
end: 150
orientation: inline

BBa_K1362400
type: SequenceAnnotation
persistent identity: annotation2427481
display id: annotation2427481
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427481/range2427481/1
component: BBa_K1362400

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type: Range
persistent identity: range2427481
display id: range2427481
version: 1
direction: direction/reverse
top level: CircNpu
start: 1231
end: 1532
orientation: inline

BBa_K1362090
type: SequenceAnnotation
persistent identity: annotation2427471
display id: annotation2427471
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427471/range2427471/1
component: BBa_K1362090

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type: Range
persistent identity: range2427471
display id: range2427471
version: 1
direction: direction/reverse
top level: CircNpu
start: 1
end: 29
orientation: inline

BBa_K1362401
type: SequenceAnnotation
persistent identity: annotation2427474
display id: annotation2427474
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427474/range2427474/1
component: BBa_K1362401

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type: Range
persistent identity: range2427474
display id: range2427474
version: 1
direction: direction/reverse
top level: CircNpu
start: 39
end: 139
orientation: inline

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2427480
display id: annotation2427480
version: 1
top level: CircNpu
location: https://synbiohub.org/public/igem/BBa_K1362000/annotation2427480/range2427480/1
component: BBa_K1362415

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type: Range
persistent identity: range2427480
display id: range2427480
version: 1
direction: direction/reverse
top level: CircNpu
start: 1227
end: 1230
orientation: inline

BBa_K1499201
type: ComponentDefinition
persistent identity: BBa K1499201
display id: BBa_K1499201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Deinococcus radiodurans uracil-DNA glycosylase 1
creator: Raman Nelakanti
created: 2014-08-24T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1879_
m user id: 0
owner id: 11095
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: TODO
mutable notes: TODO
mutable provenance: Deinococcus radiodurans
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TAA Stop
sequence annotation: uracil-DNA glycosylase 1
sequence annotation: Start codon
sequence: https://synbiohub.org/public/igem/BBa_K1499201_sequence/1

TAA Stop
type: SequenceAnnotation
persistent identity: annotation2381351
display id: annotation2381351
version: 1
top level: BBa_K1499201
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1499201/annotation2381351/range2381351/1

https://synbiohub.org/public/igem/BBa_K1499201/annotation2381351/range2381351/1
type: Range
persistent identity: range2381351
display id: range2381351
version: 1
direction: direction/forward
top level: BBa_K1499201
start: 742
end: 744
orientation: inline

uracil-DNA glycosylase 1
type: SequenceAnnotation
persistent identity: annotation2429020
display id: annotation2429020
version: 1
top level: BBa_K1499201
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1499201/annotation2429020/range2429020/1

https://synbiohub.org/public/igem/BBa_K1499201/annotation2429020/range2429020/1
type: Range
persistent identity: range2429020
display id: range2429020
version: 1
direction: direction/forward
top level: BBa_K1499201
start: 1
end: 744
orientation: inline

Start codon
type: SequenceAnnotation
persistent identity: annotation2381350
display id: annotation2381350
version: 1
top level: BBa_K1499201
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1499201/annotation2381350/range2381350/1

https://synbiohub.org/public/igem/BBa_K1499201/annotation2381350/range2381350/1
type: Range
persistent identity: range2381350
display id: range2381350
version: 1
direction: direction/forward
top level: BBa_K1499201
start: 1
end: 3
orientation: inline

BBa_K1333306
type: ComponentDefinition
persistent identity: BBa K1333306
display id: BBa_K1333306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13453-Rose42-PⅧ
creator: Runwen Yao
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15873
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K115001
component: BBa_I13453
component: BBa_K1333005
sequence annotation: BBa_K1333005
sequence annotation: BBa_I13453
sequence annotation: BBa_K115001
sequence: https://synbiohub.org/public/igem/BBa_K1333306_sequence/1

BBa_K115001
type: Component
persistent identity: component2386230
display id: component2386230
version: 1
top level: BBa_K1333306
access: public
definition: https://synbiohub.org/public/igem/BBa_K115001/1

BBa_I13453
type: Component
persistent identity: component2386223
display id: component2386223
version: 1
top level: BBa_K1333306
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K1333005
type: Component
persistent identity: component2386233
display id: component2386233
version: 1
top level: BBa_K1333306
access: public
definition: BBa_K1333005

BBa_K1333005
type: SequenceAnnotation
persistent identity: annotation2386233
display id: annotation2386233
version: 1
top level: BBa_K1333306
location: https://synbiohub.org/public/igem/BBa_K1333306/annotation2386233/range2386233/1
component: BBa_K1333005

https://synbiohub.org/public/igem/BBa_K1333306/annotation2386233/range2386233/1
type: Range
persistent identity: range2386233
display id: range2386233
version: 1
direction: direction/reverse
top level: BBa_K1333306
start: 241
end: 495
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2386223
display id: annotation2386223
version: 1
top level: BBa_K1333306
location: https://synbiohub.org/public/igem/BBa_K1333306/annotation2386223/range2386223/1
component: BBa_I13453

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type: Range
persistent identity: range2386223
display id: range2386223
version: 1
direction: direction/reverse
top level: BBa_K1333306
start: 1
end: 130
orientation: inline

BBa_K115001
type: SequenceAnnotation
persistent identity: annotation2386230
display id: annotation2386230
version: 1
top level: BBa_K1333306
location: https://synbiohub.org/public/igem/BBa_K1333306/annotation2386230/range2386230/1
component: BBa_K115001

https://synbiohub.org/public/igem/BBa_K1333306/annotation2386230/range2386230/1
type: Range
persistent identity: range2386230
display id: range2386230
version: 1
direction: direction/reverse
top level: BBa_K1333306
start: 139
end: 234
orientation: inline

BBa_K1418041
type: ComponentDefinition
persistent identity: BBa K1418041
display id: BBa_K1418041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CFP(ns)
creator: Cody Maxfield
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:12:01Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1796_
m user id: 4206
owner id: 13965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CFP
mutable notes: RFC 23
mutable provenance: CFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CFP
sequence annotation: atg
sequence: https://synbiohub.org/public/igem/BBa_K1418041_sequence/1

CFP
type: SequenceAnnotation
persistent identity: annotation2421402
display id: annotation2421402
version: 1
top level: BBa_K1418041
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1418041/annotation2421402/range2421402/1

https://synbiohub.org/public/igem/BBa_K1418041/annotation2421402/range2421402/1
type: Range
persistent identity: range2421402
display id: range2421402
version: 1
direction: direction/forward
top level: BBa_K1418041
start: 4
end: 717
orientation: inline

atg
type: SequenceAnnotation
persistent identity: annotation2421390
display id: annotation2421390
version: 1
top level: BBa_K1418041
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1418041/annotation2421390/range2421390/1

https://synbiohub.org/public/igem/BBa_K1418041/annotation2421390/range2421390/1
type: Range
persistent identity: range2421390
display id: range2421390
version: 1
direction: direction/forward
top level: BBa_K1418041
start: 1
end: 3
orientation: inline

BBa_K1493504
type: ComponentDefinition
persistent identity: BBa K1493504
display id: BBa_K1493504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet+GFP
creator: Michiel Herpers
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
group u list: _1873_
m user id: 0
owner id: 21281
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13504
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_K1493504_sequence/1

BBa_I13504
type: Component
persistent identity: component2396320
display id: component2396320
version: 1
top level: BBa_K1493504
access: public
definition: https://synbiohub.org/public/igem/BBa_I13504/1

BBa_R0040
type: Component
persistent identity: component2396306
display id: component2396306
version: 1
top level: BBa_K1493504
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2396306
display id: annotation2396306
version: 1
top level: BBa_K1493504
location: https://synbiohub.org/public/igem/BBa_K1493504/annotation2396306/range2396306/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1493504/annotation2396306/range2396306/1
type: Range
persistent identity: range2396306
display id: range2396306
version: 1
direction: direction/forward
top level: BBa_K1493504
start: 1
end: 54
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2396320
display id: annotation2396320
version: 1
top level: BBa_K1493504
location: https://synbiohub.org/public/igem/BBa_K1493504/annotation2396320/range2396320/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K1493504/annotation2396320/range2396320/1
type: Range
persistent identity: range2396320
display id: range2396320
version: 1
direction: direction/reverse
top level: BBa_K1493504
start: 63
end: 937
orientation: inline

BBa_K1333106
type: ComponentDefinition
persistent identity: BBa K1333106
display id: BBa_K1333106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS umuD'
creator: Yanwen Xie
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: umud'
mutable notes: umud'
mutable provenance: umud'
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1333109
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1333109
sequence: https://synbiohub.org/public/igem/BBa_K1333106_sequence/1

BBa_K1333109
type: Component
persistent identity: component2429534
display id: component2429534
version: 1
top level: BBa_K1333106
access: public
definition: https://synbiohub.org/public/igem/BBa_K1333109/1

BBa_B0034
type: Component
persistent identity: component2429532
display id: component2429532
version: 1
top level: BBa_K1333106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2429532
display id: annotation2429532
version: 1
top level: BBa_K1333106
location: https://synbiohub.org/public/igem/BBa_K1333106/annotation2429532/range2429532/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1333106/annotation2429532/range2429532/1
type: Range
persistent identity: range2429532
display id: range2429532
version: 1
direction: direction/reverse
top level: BBa_K1333106
start: 1
end: 12
orientation: inline

BBa_K1333109
type: SequenceAnnotation
persistent identity: annotation2429534
display id: annotation2429534
version: 1
top level: BBa_K1333106
location: https://synbiohub.org/public/igem/BBa_K1333106/annotation2429534/range2429534/1
component: BBa_K1333109

https://synbiohub.org/public/igem/BBa_K1333106/annotation2429534/range2429534/1
type: Range
persistent identity: range2429534
display id: range2429534
version: 1
direction: direction/reverse
top level: BBa_K1333106
start: 19
end: 369
orientation: inline

BBa_K1478000
type: ComponentDefinition
persistent identity: BBa K1478000
display id: BBa_K1478000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Expa4 plant secretion signal, localizes to extracellular space
creator: Fabian Fr??mling
created: 2014-09-12T11:00:00Z
modified: 2015-05-27T02:12:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1858_
m user id: 4206
owner id: 20973
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for first 20 aminoacids of plant protein Exansin4. Secretion signal for plants (derived from A. thaliana). When fused to a protein, directs the protein to extracellular space.
mutable notes: Sequence was optimized for Escherichia coli and Arabidopsis thaliana codon usage.
mutable provenance: Derived from Expansin4 from Arabidopsis thaliana(NP_181500).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K1478000_sequence/1

BBa_K1365000
type: ComponentDefinition
persistent identity: BBa K1365000
display id: BBa_K1365000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NisA
creator: Sandra Mous
created: 2014-09-22T11:00:00Z
modified: 2015-05-08T01:10:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1741_
m user id: 0
owner id: 23000
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: NisA codes for the nisin precursor. Together with the genes NisB, NisT, NisC, NisP, NisR and NisK it is responsible for producing the lantibiotic nisin in Lactococcus lactis. Can be used in the Lactococcus lactis NZ9800, a strain with a deletion in the original NisA gene, to let it produce nisin.
mutable notes: -
mutable provenance: Obtained by PCR on the genome of L. lactis NZ9700, a nisin producing strain.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: NisA
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1365000_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2385397
display id: annotation2385397
version: 1
top level: BBa_K1365000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1365000/annotation2385397/range2385397/1

https://synbiohub.org/public/igem/BBa_K1365000/annotation2385397/range2385397/1
type: Range
persistent identity: range2385397
display id: range2385397
version: 1
direction: direction/forward
top level: BBa_K1365000
start: 1
end: 3
orientation: inline

NisA
type: SequenceAnnotation
persistent identity: annotation2385399
display id: annotation2385399
version: 1
top level: BBa_K1365000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1365000/annotation2385399/range2385399/1

https://synbiohub.org/public/igem/BBa_K1365000/annotation2385399/range2385399/1
type: Range
persistent identity: range2385399
display id: range2385399
version: 1
direction: direction/forward
top level: BBa_K1365000
start: 1
end: 174
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2385398
display id: annotation2385398
version: 1
top level: BBa_K1365000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1365000/annotation2385398/range2385398/1

https://synbiohub.org/public/igem/BBa_K1365000/annotation2385398/range2385398/1
type: Range
persistent identity: range2385398
display id: range2385398
version: 1
direction: direction/forward
top level: BBa_K1365000
start: 172
end: 174
orientation: inline

BBa_K1362056
type: ComponentDefinition
persistent identity: BBa K1362056
display id: BBa_K1362056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Myristoilation signal sequence as RFC[105] N-terminal insert
creator: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1738_
m user id: 0
owner id: 22830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1362444
component: BBa_K1362425
component: BBa_K1362423
component: BBa_I712026
component: BBa_K1362415
sequence annotation: BBa_K1362415
sequence annotation: BBa_I712026
sequence annotation: BBa_K1362423
sequence annotation: BBa_K1362425
sequence annotation: BBa_K1362444
sequence: https://synbiohub.org/public/igem/BBa_K1362056_sequence/1

BBa_K1362444
type: Component
persistent identity: component2409720
display id: component2409720
version: 1
top level: BBa_K1362056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362444/1

BBa_K1362425
type: Component
persistent identity: component2409718
display id: component2409718
version: 1
top level: BBa_K1362056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362425/1

BBa_K1362423
type: Component
persistent identity: component2409722
display id: component2409722
version: 1
top level: BBa_K1362056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362423/1

BBa_I712026
type: Component
persistent identity: component2409719
display id: component2409719
version: 1
top level: BBa_K1362056
access: public
definition: https://synbiohub.org/public/igem/BBa_I712026/1

BBa_K1362415
type: Component
persistent identity: component2409721
display id: component2409721
version: 1
top level: BBa_K1362056
access: public
definition: https://synbiohub.org/public/igem/BBa_K1362415/1

BBa_K1362415
type: SequenceAnnotation
persistent identity: annotation2409721
display id: annotation2409721
version: 1
top level: BBa_K1362056
location: https://synbiohub.org/public/igem/BBa_K1362056/annotation2409721/range2409721/1
component: BBa_K1362415

https://synbiohub.org/public/igem/BBa_K1362056/annotation2409721/range2409721/1
type: Range
persistent identity: range2409721
display id: range2409721
version: 1
direction: direction/reverse
top level: BBa_K1362056
start: 47
end: 50
orientation: inline

BBa_I712026
type: SequenceAnnotation
persistent identity: annotation2409719
display id: annotation2409719
version: 1
top level: BBa_K1362056
location: https://synbiohub.org/public/igem/BBa_K1362056/annotation2409719/range2409719/1
component: BBa_I712026

https://synbiohub.org/public/igem/BBa_K1362056/annotation2409719/range2409719/1
type: Range
persistent identity: range2409719
display id: range2409719
version: 1
direction: direction/reverse
top level: BBa_K1362056
start: 8
end: 28
orientation: inline

BBa_K1362423
type: SequenceAnnotation
persistent identity: annotation2409722
display id: annotation2409722
version: 1
top level: BBa_K1362056
location: https://synbiohub.org/public/igem/BBa_K1362056/annotation2409722/range2409722/1
component: BBa_K1362423

https://synbiohub.org/public/igem/BBa_K1362056/annotation2409722/range2409722/1
type: Range
persistent identity: range2409722
display id: range2409722
version: 1
direction: direction/reverse
top level: BBa_K1362056
start: 51
end: 57
orientation: inline

BBa_K1362425
type: SequenceAnnotation
persistent identity: annotation2409718
display id: annotation2409718
version: 1
top level: BBa_K1362056
location: https://synbiohub.org/public/igem/BBa_K1362056/annotation2409718/range2409718/1
component: BBa_K1362425

https://synbiohub.org/public/igem/BBa_K1362056/annotation2409718/range2409718/1
type: Range
persistent identity: range2409718
display id: range2409718
version: 1
direction: direction/reverse
top level: BBa_K1362056
start: 1
end: 6
orientation: inline

BBa_K1362444
type: SequenceAnnotation
persistent identity: annotation2409720
display id: annotation2409720
version: 1
top level: BBa_K1362056
location: https://synbiohub.org/public/igem/BBa_K1362056/annotation2409720/range2409720/1
component: BBa_K1362444

https://synbiohub.org/public/igem/BBa_K1362056/annotation2409720/range2409720/1
type: Range
persistent identity: range2409720
display id: range2409720
version: 1
direction: direction/reverse
top level: BBa_K1362056
start: 29
end: 46
orientation: inline

BBa_K1401008
type: ComponentDefinition
persistent identity: BBa K1401008
display id: BBa_K1401008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tandem Promoter pBad-pTet
creator: Kathleen Lewis
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
group u list: _1779_
m user id: 0
owner id: 22443
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This tandem promoter was created using the MoClo assembly method. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. The promoter is induced by atc and arabinose. The characterization data will be updated before the jamboree.
mutable notes: We looked through the literature and decided to attach the two promoters without any extra sequence between them
mutable provenance: This was formed using BBa_I13453 and BBa_R0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1401008_sequence/1

BBa_K1456004
type: ComponentDefinition
persistent identity: BBa K1456004
display id: BBa_K1456004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hypoxia Response Element(HRE)
creator: safa tapan
created: 2014-07-20T11:00:00Z
modified: 2015-05-08T01:10:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1834_
m user id: 0
owner id: 17366
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: HRE is a enhancer and regulate gene transcription depend on hypoxia.
mutable notes: This part will help our cells to sense hypoxic conditions in order to activate our other vital parts that allow oxygen re-support.
mutable provenance: We clonned this part from human hepatocellular carcinoma cell genome cDNA
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1456004_sequence/1

lac Or2-62
type: ComponentDefinition
persistent identity: BBa I12210
display id: BBa_I12210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: plac Or2-62 (positive)
creator: rwald
created: 2004-08-06T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 120
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a modification of the standard plac operon with everything before the -35 site removed, the Or2 site from the lambda promoter inserted at the beginning, and a 17-bp spacer added between the two.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: cI OR2 (lambda)
sequence annotation: spacer
sequence: https://synbiohub.org/public/igem/BBa_I12210_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1031725
display id: annotation1031725
version: 1
top level: lac Or2-62
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031725/range1031725/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031725/range1031725/1
type: Range
persistent identity: range1031725
display id: range1031725
version: 1
direction: direction/forward
top level: lac Or2-62
start: 35
end: 40
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1031726
display id: annotation1031726
version: 1
top level: lac Or2-62
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031726/range1031726/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031726/range1031726/1
type: Range
persistent identity: range1031726
display id: range1031726
version: 1
direction: direction/forward
top level: lac Or2-62
start: 59
end: 65
orientation: inline

cI OR2 (lambda)
type: SequenceAnnotation
persistent identity: annotation1031724
display id: annotation1031724
version: 1
top level: lac Or2-62
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031724/range1031724/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031724/range1031724/1
type: Range
persistent identity: range1031724
display id: range1031724
version: 1
direction: direction/forward
top level: lac Or2-62
start: 1
end: 17
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1031727
display id: annotation1031727
version: 1
top level: lac Or2-62
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I12210/annotation1031727/range1031727/1

https://synbiohub.org/public/igem/BBa_I12210/annotation1031727/range1031727/1
type: Range
persistent identity: range1031727
display id: range1031727
version: 1
direction: direction/forward
top level: lac Or2-62
start: 18
end: 34
orientation: inline

BBa_K1415106
type: ComponentDefinition
persistent identity: BBa K1415106
display id: BBa_K1415106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons+B0034+PBAN(Helicoverpa armigera Hubner )
creator: HO, TSUNG YU
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part has a constitutive promoter, so our PBAN(Lymantria dispar) can constitutive express.
mutable notes: No
mutable provenance: Assemble
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J23101
component: BBa_K1415006
component: BBa_B0034
sequence annotation: BBa_K1415006
sequence annotation: BBa_B0034
sequence annotation: BBa_J23101
sequence: https://synbiohub.org/public/igem/BBa_K1415106_sequence/1

BBa_J23101
type: Component
persistent identity: component2393980
display id: component2393980
version: 1
top level: BBa_K1415106
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K1415006
type: Component
persistent identity: component2393984
display id: component2393984
version: 1
top level: BBa_K1415106
access: public
definition: BBa_K1415006

BBa_B0034
type: Component
persistent identity: component2393982
display id: component2393982
version: 1
top level: BBa_K1415106
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1415006
type: SequenceAnnotation
persistent identity: annotation2393984
display id: annotation2393984
version: 1
top level: BBa_K1415106
location: https://synbiohub.org/public/igem/BBa_K1415106/annotation2393984/range2393984/1
component: BBa_K1415006

https://synbiohub.org/public/igem/BBa_K1415106/annotation2393984/range2393984/1
type: Range
persistent identity: range2393984
display id: range2393984
version: 1
direction: direction/reverse
top level: BBa_K1415106
start: 62
end: 170
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393982
display id: annotation2393982
version: 1
top level: BBa_K1415106
location: https://synbiohub.org/public/igem/BBa_K1415106/annotation2393982/range2393982/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1415106/annotation2393982/range2393982/1
type: Range
persistent identity: range2393982
display id: range2393982
version: 1
direction: direction/reverse
top level: BBa_K1415106
start: 44
end: 55
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2393980
display id: annotation2393980
version: 1
top level: BBa_K1415106
location: https://synbiohub.org/public/igem/BBa_K1415106/annotation2393980/range2393980/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1415106/annotation2393980/range2393980/1
type: Range
persistent identity: range2393980
display id: range2393980
version: 1
direction: direction/reverse
top level: BBa_K1415106
start: 1
end: 35
orientation: inline

BBa_K1351011
type: ComponentDefinition
persistent identity: BBa K1351011
display id: BBa_K1351011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Bacillus subtilis sortase YhcS
creator: Mona Dotzler
created: 2014-10-04T11:00:00Z
modified: 2015-06-01T12:55:40Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ''Bacillus subtilis'' sortase YhcS (UniProt [http://www.uniprot.org/uniprot/P54603 P54603]), responsible for anchoring its substrae YhcR (UniProt [http://www.uniprot.org/uniprot/P54602 P54602]) covalently to the peptidoglycan wall of ''B. subtilis''. A YhcR-based surface display system has been shown to covalently anchor heterologous proteins to the ''B. subtilis'' cell wall (Liew ''et. al.'', 2012). It was used in the [http://2014.igem.org/Team:LMU-Munich/Project/Bakillus BaKillus project] to display pathogen-specific peptides and mediate adhesion of ''B. subtlis'' to pathogens. It consists of four components: the sortase substrate with a signal peptide (this part), a linker ([http://parts.igem.org/Part:BBa_K1351009 BBa_K1351009]) and a cell wall-anchoring domain ([http://parts.igem.org/Part:BBa_K1351010 BBa_K1351010]) as well as the sortase YhcS itself ([http://parts.igem.org/Part:BBa_K1351011 BBa_K1351011]), whose overexpression increases the efficiency of the surface display. This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix: {| |prefix with EcoRI, NotI, XbaI, SD and NgoMIV: |GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC |- |suffix with AgeI, SpeI, NotI and PstI: |ACCGGTTAATACTAGTAGCGGCCGCCTGCAG |} Sites of restriction enzymes generating compatible overhangs have the same color: EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.
mutable notes: none
mutable provenance: This part was generated by amplification from ''B. subtilis'' W168 gDNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3. YhcS_ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC GTGAAAAAAGTTATTCCACTATTCATCATTGC YhcS_SNP_rev: gatcCTGCAGcggccgctACTAGTa TTAAGTCACTCGTTTTCCATATATAATATAGCG An EcoRI site was removed by changing T to C at position 399 without altering the protein sequence by fusion PCR with the following mutagenesis primers: YhcS_EcoRI435mut_fwd: CGTCTTCTCCTGTCATATGGAGAATTtACATATGAAATTGTAAAAACAAAAATTGTCG YhcS_EcoRI435mut_rev: CGACAATTTTTGTTTTTACAATTTCATATGTaAATTCTCCATATGACAGGAGAAGACG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351011_sequence/1

BBa_K1321100
type: ComponentDefinition
persistent identity: BBa K1321100
display id: BBa_K1321100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phytochelatin (PC) EC20 fused to linker-dCBD with T7 promoter
creator: Xenia Spencer-Milnes
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:18:05Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20780
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Phytochelatin EC(20) +CBD2 with T7. TBC
mutable notes: Phytochelatin EC(20) +CBD2 with T7. TBC
mutable provenance: Phytochelatin EC(20) +CBD2 with T7. TBC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321340
component: BBa_K1321338
component: BBa_K1321005
component: BBa_B0105
component: BBa_K1319106
sequence annotation: BBa_K1321340
sequence annotation: BBa_K1319106
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321338
sequence annotation: BBa_K1321005
sequence: https://synbiohub.org/public/igem/BBa_K1321100_sequence/1

BBa_K1321340
type: Component
persistent identity: component2415255
display id: component2415255
version: 1
top level: BBa_K1321100
access: public
definition: BBa_K1321340

BBa_K1321338
type: Component
persistent identity: component2415247
display id: component2415247
version: 1
top level: BBa_K1321100
access: public
definition: BBa_K1321338

BBa_K1321005
type: Component
persistent identity: component2415251
display id: component2415251
version: 1
top level: BBa_K1321100
access: public
definition: BBa_K1321005

BBa_B0105
type: Component
persistent identity: component2415253
display id: component2415253
version: 1
top level: BBa_K1321100
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1319106
type: Component
persistent identity: component2415249
display id: component2415249
version: 1
top level: BBa_K1321100
access: public
definition: https://synbiohub.org/public/igem/BBa_K1319106/1

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2415255
display id: annotation2415255
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415255/range2415255/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415255/range2415255/1
type: Range
persistent identity: range2415255
display id: range2415255
version: 1
direction: direction/reverse
top level: BBa_K1321100
start: 188
end: 553
orientation: inline

BBa_K1319106
type: SequenceAnnotation
persistent identity: annotation2415249
display id: annotation2415249
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415249/range2415249/1
component: BBa_K1319106

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415249/range2415249/1
type: Range
persistent identity: range2415249
display id: range2415249
version: 1
direction: direction/reverse
top level: BBa_K1321100
start: 44
end: 58
orientation: inline

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2415253
display id: annotation2415253
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415253/range2415253/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415253/range2415253/1
type: Range
persistent identity: range2415253
display id: range2415253
version: 1
direction: direction/reverse
top level: BBa_K1321100
start: 182
end: 187
orientation: inline

BBa_K1321338
type: SequenceAnnotation
persistent identity: annotation2415247
display id: annotation2415247
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415247/range2415247/1
component: BBa_K1321338

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415247/range2415247/1
type: Range
persistent identity: range2415247
display id: range2415247
version: 1
direction: direction/reverse
top level: BBa_K1321100
start: 1
end: 43
orientation: inline

BBa_K1321005
type: SequenceAnnotation
persistent identity: annotation2415251
display id: annotation2415251
version: 1
top level: BBa_K1321100
location: https://synbiohub.org/public/igem/BBa_K1321100/annotation2415251/range2415251/1
component: BBa_K1321005

https://synbiohub.org/public/igem/BBa_K1321100/annotation2415251/range2415251/1
type: Range
persistent identity: range2415251
display id: range2415251
version: 1
direction: direction/reverse
top level: BBa_K1321100
start: 59
end: 181
orientation: inline

BBa_K1343021
type: ComponentDefinition
persistent identity: BBa K1343021
display id: BBa_K1343021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux->amilCP->lambda CI
creator: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1718_
m user id: 0
owner id: 20669
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The promoter Plux in induced by the dimer AHL-luxR. As a result, amilCP, a blue chromophoire protein, is synthesized. Lambda phage CI is a repressor of the promoter PCI.
mutable notes: r
mutable provenance: BBa_R0062: Plux, BBa_B0030: Strong RBS, BBa_K592009: amilCP, BBa_B0010:T1 (terminator) from E. coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1343021_sequence/1

BBa_K1315010
type: ComponentDefinition
persistent identity: BBa K1315010
display id: BBa_K1315010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pseudomonas AutoInducer 1 (PAI-1) Biosensor
creator: Dimitrios Michailidis
created: 2014-09-25T11:00:00Z
modified: 2015-05-08T01:09:50Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1690_
m user id: 0
owner id: 21812
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a composite part that consists of 4 biobricks. This is an alternative PA1 Activated System from Bba_K1315009 that instead uses pluxR (Bba_R0062). In the front it has ptet(Bba_R0040) which is constitutively on. This produces LasR all the time with lasR(Bba_C0179) next in the sequence. Only in the presence of the Pseudomonas Autoinducer 1 (PA1), LasR forms an complex with PA1 and it can then activate the pluxR(Bba_R0062) promoter that leads to the production of GFP(Bba_E0040).
mutable notes: All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.
mutable provenance: All the parts have come from existing iGEM distribution kits.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_R0062
component: BBa_E0040
component: BBa_C0179
sequence annotation: BBa_R0040
sequence annotation: BBa_C0179
sequence annotation: BBa_E0040
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K1315010_sequence/1

BBa_R0040
type: Component
persistent identity: component2387063
display id: component2387063
version: 1
top level: BBa_K1315010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0062
type: Component
persistent identity: component2387070
display id: component2387070
version: 1
top level: BBa_K1315010
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_E0040
type: Component
persistent identity: component2387075
display id: component2387075
version: 1
top level: BBa_K1315010
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_C0179
type: Component
persistent identity: component2387068
display id: component2387068
version: 1
top level: BBa_K1315010
access: public
definition: https://synbiohub.org/public/igem/BBa_C0179/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2387063
display id: annotation2387063
version: 1
top level: BBa_K1315010
location: https://synbiohub.org/public/igem/BBa_K1315010/annotation2387063/range2387063/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K1315010/annotation2387063/range2387063/1
type: Range
persistent identity: range2387063
display id: range2387063
version: 1
direction: direction/forward
top level: BBa_K1315010
start: 1
end: 54
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2387068
display id: annotation2387068
version: 1
top level: BBa_K1315010
location: https://synbiohub.org/public/igem/BBa_K1315010/annotation2387068/range2387068/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K1315010/annotation2387068/range2387068/1
type: Range
persistent identity: range2387068
display id: range2387068
version: 1
direction: direction/reverse
top level: BBa_K1315010
start: 61
end: 783
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2387075
display id: annotation2387075
version: 1
top level: BBa_K1315010
location: https://synbiohub.org/public/igem/BBa_K1315010/annotation2387075/range2387075/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1315010/annotation2387075/range2387075/1
type: Range
persistent identity: range2387075
display id: range2387075
version: 1
direction: direction/reverse
top level: BBa_K1315010
start: 853
end: 1572
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2387070
display id: annotation2387070
version: 1
top level: BBa_K1315010
location: https://synbiohub.org/public/igem/BBa_K1315010/annotation2387070/range2387070/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1315010/annotation2387070/range2387070/1
type: Range
persistent identity: range2387070
display id: range2387070
version: 1
direction: direction/forward
top level: BBa_K1315010
start: 792
end: 846
orientation: inline

BBa_K1333005
type: ComponentDefinition
persistent identity: BBa K1333005
display id: BBa_K1333005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: M13 bacteriophage g8p with a FLAG tag on C-terminal
creator: Huang Junxiang, Wang Xizi
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1708_
m user id: 0
owner id: 15838
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: pVIII with a FLAG tag on C-terminal
mutable notes: pVIII with a FLAG tag on C-terminal
mutable provenance: pVIII with a FLAG tag on C-terminal
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: FLAG
sequence annotation: g8p
sequence: https://synbiohub.org/public/igem/BBa_K1333005_sequence/1

FLAG
type: SequenceAnnotation
persistent identity: annotation2386089
display id: annotation2386089
version: 1
top level: BBa_K1333005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1333005/annotation2386089/range2386089/1

https://synbiohub.org/public/igem/BBa_K1333005/annotation2386089/range2386089/1
type: Range
persistent identity: range2386089
display id: range2386089
version: 1
direction: direction/forward
top level: BBa_K1333005
start: 229
end: 255
orientation: inline

g8p
type: SequenceAnnotation
persistent identity: annotation2386088
display id: annotation2386088
version: 1
top level: BBa_K1333005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1333005/annotation2386088/range2386088/1

https://synbiohub.org/public/igem/BBa_K1333005/annotation2386088/range2386088/1
type: Range
persistent identity: range2386088
display id: range2386088
version: 1
direction: direction/forward
top level: BBa_K1333005
start: 1
end: 219
orientation: inline

BBa_K1351000
type: ComponentDefinition
persistent identity: BBa K1351000
display id: BBa_K1351000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: beta-Neurexin-derived peptide binding to S. aureus adhesin SdrC
creator: Mona Dotzler
created: 2014-09-14T11:00:00Z
modified: 2015-06-01T12:53:55Z
discontinued: false
dominant: false
group u list: _1726_
m user id: 4206
owner id: 20048
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Short peptide of 11 amino acids corresponding to the N-terminus of the human protein neurexin beta-1. It includes the binding site (HHIHHFH) for the Staphylococcus aureus adhesin SdrC. Displayed on the surface of Bacillus subtilis, it shoud enable B. subtilis to bind specifically to S. aureus.
mutable notes: Oligos were designed as precut by EcoRI and PstI to shorten the synthesized fragments and to simplify the cloning into pSB1C3.
mutable provenance: The part was generated by annealing of artificially synthesized oligonucletides: Nrx1b_ENX_SD_Ngo_precutEP_fwd: AATTCgcggccgctTCTAGAgtaaggaggaaGCCGGCATG GCGCACCACATTCACCACTTTCACGGATCA ACCGGTtaatACTAGTagcggccgCTGCA Nrx1b_SNP_Age_precutEP_rev: GcggccgctACTAGTattaACCGGT TGATCCGTGAAAGTGGTGAATGTGGTGCGC CATGCCGGCttcctccttacTCTAGAagcggccgcG
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351000_sequence/1

BBa_K1333304
type: ComponentDefinition
persistent identity: BBa K1333304
display id: BBa_K1333304
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I13453-PrfA
creator: Runwen Yao
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1708_
m user id: 0
owner id: 15873
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is a combination of a pBAD promoter(BBa_I13453), RNA thermometer(BBa_K115003)
mutable provenance: The source of the part will be its components.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K115003
component: BBa_I13453
sequence annotation: BBa_K115003
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1333304_sequence/1

BBa_K115003
type: Component
persistent identity: component2385932
display id: component2385932
version: 1
top level: BBa_K1333304
access: public
definition: https://synbiohub.org/public/igem/BBa_K115003/1

BBa_I13453
type: Component
persistent identity: component2385929
display id: component2385929
version: 1
top level: BBa_K1333304
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K115003
type: SequenceAnnotation
persistent identity: annotation2385932
display id: annotation2385932
version: 1
top level: BBa_K1333304
location: https://synbiohub.org/public/igem/BBa_K1333304/annotation2385932/range2385932/1
component: BBa_K115003

https://synbiohub.org/public/igem/BBa_K1333304/annotation2385932/range2385932/1
type: Range
persistent identity: range2385932
display id: range2385932
version: 1
direction: direction/reverse
top level: BBa_K1333304
start: 139
end: 247
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2385929
display id: annotation2385929
version: 1
top level: BBa_K1333304
location: https://synbiohub.org/public/igem/BBa_K1333304/annotation2385929/range2385929/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1333304/annotation2385929/range2385929/1
type: Range
persistent identity: range2385929
display id: range2385929
version: 1
direction: direction/reverse
top level: BBa_K1333304
start: 1
end: 130
orientation: inline

BBa_K1465302
type: ComponentDefinition
persistent identity: BBa K1465302
display id: BBa_K1465302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Isobutanol pathway
creator: iGEM-Team Bielefeld 2014
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1844_
m user id: 0
owner id: 20850
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: alsS_ilvC_ilvD_kivD
mutable notes: bb
mutable provenance: bb
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: ilvC
sequence annotation: RBS
sequence annotation: kivD
sequence annotation: ilvD
sequence annotation: alsS
sequence: https://synbiohub.org/public/igem/BBa_K1465302_sequence/1

ilvC
type: SequenceAnnotation
persistent identity: annotation2404135
display id: annotation2404135
version: 1
top level: BBa_K1465302
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1465302/annotation2404135/range2404135/1

https://synbiohub.org/public/igem/BBa_K1465302/annotation2404135/range2404135/1
type: Range
persistent identity: range2404135
display id: range2404135
version: 1
direction: direction/forward
top level: BBa_K1465302
start: 1741
end: 3216
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2404140
display id: annotation2404140
version: 1
top level: BBa_K1465302
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1465302/annotation2404140/range2404140/1

https://synbiohub.org/public/igem/BBa_K1465302/annotation2404140/range2404140/1
type: Range
persistent identity: range2404140
display id: range2404140
version: 1
direction: direction/forward
top level: BBa_K1465302
start: 3217
end: 3228
orientation: inline

kivD
type: SequenceAnnotation
persistent identity: annotation2404138
display id: annotation2404138
version: 1
top level: BBa_K1465302
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1465302/annotation2404138/range2404138/1

https://synbiohub.org/public/igem/BBa_K1465302/annotation2404138/range2404138/1
type: Range
persistent identity: range2404138
display id: range2404138
version: 1
direction: direction/forward
top level: BBa_K1465302
start: 5092
end: 6738
orientation: inline

ilvD
type: SequenceAnnotation
persistent identity: annotation2404137
display id: annotation2404137
version: 1
top level: BBa_K1465302
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1465302/annotation2404137/range2404137/1

https://synbiohub.org/public/igem/BBa_K1465302/annotation2404137/range2404137/1
type: Range
persistent identity: range2404137
display id: range2404137
version: 1
direction: direction/forward
top level: BBa_K1465302
start: 3229
end: 5079
orientation: inline

alsS
type: SequenceAnnotation
persistent identity: annotation2404132
display id: annotation2404132
version: 1
top level: BBa_K1465302
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1465302/annotation2404132/range2404132/1

https://synbiohub.org/public/igem/BBa_K1465302/annotation2404132/range2404132/1
type: Range
persistent identity: range2404132
display id: range2404132
version: 1
direction: direction/forward
top level: BBa_K1465302
start: 13
end: 1728
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2404139
display id: annotation2404139
version: 1
top level: BBa_K1465302
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1465302/annotation2404139/range2404139/1

https://synbiohub.org/public/igem/BBa_K1465302/annotation2404139/range2404139/1
type: Range
persistent identity: range2404139
display id: range2404139
version: 1
direction: direction/forward
top level: BBa_K1465302
start: 5080
end: 5091
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2404133
display id: annotation2404133
version: 1
top level: BBa_K1465302
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1465302/annotation2404133/range2404133/1

https://synbiohub.org/public/igem/BBa_K1465302/annotation2404133/range2404133/1
type: Range
persistent identity: range2404133
display id: range2404133
version: 1
direction: direction/forward
top level: BBa_K1465302
start: 1729
end: 1740
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2404130
display id: annotation2404130
version: 1
top level: BBa_K1465302
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1465302/annotation2404130/range2404130/1

https://synbiohub.org/public/igem/BBa_K1465302/annotation2404130/range2404130/1
type: Range
persistent identity: range2404130
display id: range2404130
version: 1
direction: direction/forward
top level: BBa_K1465302
start: 1
end: 12
orientation: inline

BBa_K1321337
type: ComponentDefinition
persistent identity: BBa K1321337
display id: BBa_K1321337
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP in Freiburg format (RFC 25)
creator: Chris N Micklem
created: 2014-10-07T11:00:00Z
modified: 2016-01-26T02:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20830
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: sfGFP in Freiburg format (RFC 25) to allow for easy use in fusion proteins.
mutable notes: OEPCR
mutable provenance: Made from existing part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: sfGFP
sequence: https://synbiohub.org/public/igem/BBa_K1321337_sequence/1

sfGFP
type: SequenceAnnotation
persistent identity: annotation2417826
display id: annotation2417826
version: 1
top level: BBa_K1321337
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1

https://synbiohub.org/public/igem/BBa_K1321337/annotation2417826/range2417826/1
type: Range
persistent identity: range2417826
display id: range2417826
version: 1
direction: direction/forward
top level: BBa_K1321337
start: 1
end: 711
orientation: inline

BBa_K1399010
type: ComponentDefinition
persistent identity: BBa K1399010
display id: BBa_K1399010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac-RFP(AAV)
creator: Anna Stikane
created: 2014-09-18T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1777_
m user id: 0
owner id: 22477
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Lactose/IPTG inducible promoter wuth RFP reporter tagged with AAV-ssrA degradation tag followed by terminator. The tagged RFP is actively degraded within cell, thus provides better temporal resolution of red fluorescence and promoter activity. Part was assembled using BrickClip assembly (BBF RFC 104) using other biobrick parts as templates. BrickClip assembly is a special case of more general Paperclip assembly method.[1] Reference [1] Trubitsyna, M., Michlewski, G., Cai, Y., Elfick, A. & French, C. E. PaperClip: rapid multi-part DNA assembly from existing libraries. Nucleic Acids Res. 1???8 (2014). doi:10.1093/nar/gku829
mutable notes: no
mutable provenance: Other biobrick parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_B0012
sequence annotation: barcode from BBa_E1010
sequence annotation: BBa_E1010 w/o stop
sequence annotation: polya
sequence annotation: BBa_R0010
sequence annotation: -10
sequence annotation: stop
sequence annotation: BBa_B0015
sequence annotation: -35
sequence annotation: T7 TE
sequence annotation: BBa_B0034
sequence annotation: BBa_K1399000
sequence annotation: CAP binding site
sequence annotation: stem_loop
sequence annotation: AAV-ssrA tag
sequence annotation: BBa_B0010
sequence annotation: conserved
sequence annotation: start
sequence annotation: LacI binding site
sequence: https://synbiohub.org/public/igem/BBa_K1399010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation2383934
display id: annotation2383934
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383934/range2383934/1
type: Range
persistent identity: range2383934
display id: range2383934
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1
end: 88
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2383950
display id: annotation2383950
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383950/range2383950/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383950/range2383950/1
type: Range
persistent identity: range2383950
display id: range2383950
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1062
end: 1102
orientation: inline

barcode from BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2386257
display id: annotation2386257
version: 1
top level: BBa_K1399010
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2386257/range2386257/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2386257/range2386257/1
type: Range
persistent identity: range2386257
display id: range2386257
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 941
end: 965
orientation: inline

BBa_E1010 w/o stop
type: SequenceAnnotation
persistent identity: annotation2383943
display id: annotation2383943
version: 1
top level: BBa_K1399010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383943/range2383943/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383943/range2383943/1
type: Range
persistent identity: range2383943
display id: range2383943
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 230
end: 901
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2383952
display id: annotation2383952
version: 1
top level: BBa_K1399010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383952/range2383952/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383952/range2383952/1
type: Range
persistent identity: range2383952
display id: range2383952
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1089
end: 1102
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2383933
display id: annotation2383933
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383933/range2383933/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383933/range2383933/1
type: Range
persistent identity: range2383933
display id: range2383933
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1
end: 200
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2383937
display id: annotation2383937
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383937/range2383937/1
type: Range
persistent identity: range2383937
display id: range2383937
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 161
end: 166
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383946
display id: annotation2383946
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383946/range2383946/1
type: Range
persistent identity: range2383946
display id: range2383946
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 938
end: 940
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383953
display id: annotation2383953
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383953/range2383953/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383953/range2383953/1
type: Range
persistent identity: range2383953
display id: range2383953
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1095
end: 1095
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2383954
display id: annotation2383954
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383954/range2383954/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383954/range2383954/1
type: Range
persistent identity: range2383954
display id: range2383954
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 974
end: 1102
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2383936
display id: annotation2383936
version: 1
top level: BBa_K1399010
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383936/range2383936/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383936/range2383936/1
type: Range
persistent identity: range2383936
display id: range2383936
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 137
end: 142
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2383951
display id: annotation2383951
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383951/range2383951/1
type: Range
persistent identity: range2383951
display id: range2383951
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 1069
end: 1088
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2383941
display id: annotation2383941
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383941/range2383941/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383941/range2383941/1
type: Range
persistent identity: range2383941
display id: range2383941
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 209
end: 220
orientation: inline

BBa_K1399000
type: SequenceAnnotation
persistent identity: annotation2383947
display id: annotation2383947
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383947/range2383947/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383947/range2383947/1
type: Range
persistent identity: range2383947
display id: range2383947
version: 1
direction: direction/reverse
top level: BBa_K1399010
start: 227
end: 940
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation2383935
display id: annotation2383935
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383935/range2383935/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383935/range2383935/1
type: Range
persistent identity: range2383935
display id: range2383935
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 89
end: 126
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2383949
display id: annotation2383949
version: 1
top level: BBa_K1399010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383949/range2383949/1
type: Range
persistent identity: range2383949
display id: range2383949
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 985
end: 1028
orientation: inline

AAV-ssrA tag
type: SequenceAnnotation
persistent identity: annotation2383944
display id: annotation2383944
version: 1
top level: BBa_K1399010
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383944/range2383944/1
type: Range
persistent identity: range2383944
display id: range2383944
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 902
end: 934
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2383948
display id: annotation2383948
version: 1
top level: BBa_K1399010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383948/range2383948/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383948/range2383948/1
type: Range
persistent identity: range2383948
display id: range2383948
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 974
end: 1053
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2383940
display id: annotation2383940
version: 1
top level: BBa_K1399010
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383940/range2383940/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383940/range2383940/1
type: Range
persistent identity: range2383940
display id: range2383940
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 213
end: 216
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383939
display id: annotation2383939
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383939/range2383939/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383939/range2383939/1
type: Range
persistent identity: range2383939
display id: range2383939
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 173
end: 173
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2383945
display id: annotation2383945
version: 1
top level: BBa_K1399010
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383945/range2383945/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383945/range2383945/1
type: Range
persistent identity: range2383945
display id: range2383945
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 935
end: 937
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation2383938
display id: annotation2383938
version: 1
top level: BBa_K1399010
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383938/range2383938/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383938/range2383938/1
type: Range
persistent identity: range2383938
display id: range2383938
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2383942
display id: annotation2383942
version: 1
top level: BBa_K1399010
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1399010/annotation2383942/range2383942/1

https://synbiohub.org/public/igem/BBa_K1399010/annotation2383942/range2383942/1
type: Range
persistent identity: range2383942
display id: range2383942
version: 1
direction: direction/forward
top level: BBa_K1399010
start: 227
end: 229
orientation: inline

BBa_K1357010
type: ComponentDefinition
persistent identity: BBa K1357010
display id: BBa_K1357010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: eRFP reporter
creator: Matthew Tucker
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:02Z
discontinued: false
dominant: false
group u list: _1733_
m user id: 0
owner id: 16772
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS + Coding + Term
mutable notes: None
mutable provenance: jellyfish
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_K1357010_sequence/1

BBa_B0015
type: Component
persistent identity: component2408723
display id: component2408723
version: 1
top level: BBa_K1357010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_E1010
type: Component
persistent identity: component2408716
display id: component2408716
version: 1
top level: BBa_K1357010
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: Component
persistent identity: component2408713
display id: component2408713
version: 1
top level: BBa_K1357010
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2408713
display id: annotation2408713
version: 1
top level: BBa_K1357010
location: https://synbiohub.org/public/igem/BBa_K1357010/annotation2408713/range2408713/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1357010/annotation2408713/range2408713/1
type: Range
persistent identity: range2408713
display id: range2408713
version: 1
direction: direction/reverse
top level: BBa_K1357010
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2408723
display id: annotation2408723
version: 1
top level: BBa_K1357010
location: https://synbiohub.org/public/igem/BBa_K1357010/annotation2408723/range2408723/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1357010/annotation2408723/range2408723/1
type: Range
persistent identity: range2408723
display id: range2408723
version: 1
direction: direction/reverse
top level: BBa_K1357010
start: 733
end: 861
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2408716
display id: annotation2408716
version: 1
top level: BBa_K1357010
location: https://synbiohub.org/public/igem/BBa_K1357010/annotation2408716/range2408716/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K1357010/annotation2408716/range2408716/1
type: Range
persistent identity: range2408716
display id: range2408716
version: 1
direction: direction/reverse
top level: BBa_K1357010
start: 19
end: 724
orientation: inline

BBa_K1467204
type: ComponentDefinition
persistent identity: BBa K1467204
display id: BBa_K1467204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GoldenGate compatible Green Flourescent Protein (GFP)
creator: Cara Deal
created: 2014-09-23T11:00:00Z
modified: 2015-06-01T07:05:42Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1846_
m user id: 4206
owner id: 21550
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: A variation of the part BBa_E0040 that has been made compatible for Golden Gate by removal of internal BsaI and BpiI recognition sequences. Fluoresces under UV light when the gene is expressed.
mutable notes: Domestication for GoldenGate by removal of internal BsaI and BpiI recognition sequences.
mutable provenance: Aequorea victoria
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1467204_sequence/1

BBa_K1402008
type: ComponentDefinition
persistent identity: BBa K1402008
display id: BBa_K1402008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FadD Promoter
creator: Chen Xi
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:10:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1780_
m user id: 0
owner id: 16730
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is sensitive to long chain acyl-CoA ,with a FadR binding site. In the absence of long chain acyl-CoA, FadR represses the transcription. In the presence of long chain acyl-CoA, FadR dissociates from the binding site so that the transcription begins. E.coli uses this promoter to regulate fatty acid metabolism.
mutable notes: no
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1402008_sequence/1

BBa_K1481002
type: ComponentDefinition
persistent identity: BBa K1481002
display id: BBa_K1481002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: sfGFP under arabinose promoter
creator: Melania Nowicka
created: 2014-10-09T11:00:00Z
modified: 2015-05-08T01:10:40Z
discontinued: false
dominant: false
group u list: _1861_
m user id: 0
owner id: 17561
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: sfGFP under arabinose promoter
mutable notes: -
mutable provenance: Synthetic + genomic E.Coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: -35 signal
sequence annotation: pBAD
sequence annotation: -10 signal
sequence annotation: O2 signal
sequence annotation: CRP binding site
sequence annotation: 5'-UTR
sequence annotation: his-sfGFP
sequence annotation: AraC binding site
sequence annotation: O1 signal
sequence annotation: AraC
sequence annotation: his-tag
sequence: https://synbiohub.org/public/igem/BBa_K1481002_sequence/1

-35 signal
type: SequenceAnnotation
persistent identity: annotation2415078
display id: annotation2415078
version: 1
top level: BBa_K1481002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2415078/range2415078/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2415078/range2415078/1
type: Range
persistent identity: range2415078
display id: range2415078
version: 1
direction: direction/forward
top level: BBa_K1481002
start: 1153
end: 1157
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2414808
display id: annotation2414808
version: 1
top level: BBa_K1481002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2414808/range2414808/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2414808/range2414808/1
type: Range
persistent identity: range2414808
display id: range2414808
version: 1
direction: direction/forward
top level: BBa_K1481002
start: 880
end: 1217
orientation: inline

-10 signal
type: SequenceAnnotation
persistent identity: annotation2415079
display id: annotation2415079
version: 1
top level: BBa_K1481002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2415079/range2415079/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2415079/range2415079/1
type: Range
persistent identity: range2415079
display id: range2415079
version: 1
direction: direction/forward
top level: BBa_K1481002
start: 1177
end: 1181
orientation: inline

O2 signal
type: SequenceAnnotation
persistent identity: annotation2415070
display id: annotation2415070
version: 1
top level: BBa_K1481002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2415070/range2415070/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2415070/range2415070/1
type: Range
persistent identity: range2415070
display id: range2415070
version: 1
direction: direction/forward
top level: BBa_K1481002
start: 1066
end: 1087
orientation: inline

CRP binding site
type: SequenceAnnotation
persistent identity: annotation2414920
display id: annotation2414920
version: 1
top level: BBa_K1481002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2414920/range2414920/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2414920/range2414920/1
type: Range
persistent identity: range2414920
display id: range2414920
version: 1
direction: direction/forward
top level: BBa_K1481002
start: 1111
end: 1121
orientation: inline

5'-UTR
type: SequenceAnnotation
persistent identity: annotation2414937
display id: annotation2414937
version: 1
top level: BBa_K1481002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2414937/range2414937/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2414937/range2414937/1
type: Range
persistent identity: range2414937
display id: range2414937
version: 1
direction: direction/forward
top level: BBa_K1481002
start: 1191
end: 1217
orientation: inline

his-sfGFP
type: SequenceAnnotation
persistent identity: annotation2414809
display id: annotation2414809
version: 1
top level: BBa_K1481002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2414809/range2414809/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2414809/range2414809/1
type: Range
persistent identity: range2414809
display id: range2414809
version: 1
direction: direction/forward
top level: BBa_K1481002
start: 1218
end: 1967
orientation: inline

AraC binding site
type: SequenceAnnotation
persistent identity: annotation2414919
display id: annotation2414919
version: 1
top level: BBa_K1481002
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2414919/range2414919/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2414919/range2414919/1
type: Range
persistent identity: range2414919
display id: range2414919
version: 1
direction: direction/forward
top level: BBa_K1481002
start: 1113
end: 1155
orientation: inline

O1 signal
type: SequenceAnnotation
persistent identity: annotation2414986
display id: annotation2414986
version: 1
top level: BBa_K1481002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2414986/range2414986/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2414986/range2414986/1
type: Range
persistent identity: range2414986
display id: range2414986
version: 1
direction: direction/forward
top level: BBa_K1481002
start: 909
end: 924
orientation: inline

AraC
type: SequenceAnnotation
persistent identity: annotation2414819
display id: annotation2414819
version: 1
top level: BBa_K1481002
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2414819/range2414819/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2414819/range2414819/1
type: Range
persistent identity: range2414819
display id: range2414819
version: 1
direction: direction/reverse
top level: BBa_K1481002
start: 1
end: 879
orientation: inline

his-tag
type: SequenceAnnotation
persistent identity: annotation2415080
display id: annotation2415080
version: 1
top level: BBa_K1481002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1481002/annotation2415080/range2415080/1

https://synbiohub.org/public/igem/BBa_K1481002/annotation2415080/range2415080/1
type: Range
persistent identity: range2415080
display id: range2415080
version: 1
direction: direction/forward
top level: BBa_K1481002
start: 1230
end: 1247
orientation: inline

BBa_K1343019
type: ComponentDefinition
persistent identity: BBa K1343019
display id: BBa_K1343019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plux->Green Renilla Luciferase->tetR
creator: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
created: 2014-10-05T11:00:00Z
modified: 2015-05-08T01:10:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1718_
m user id: 0
owner id: 20669
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The promoter Plux is induced by the binding of an AHL-luxR dimer. This bio-brick leads to the synthesis of the enzyme Green Renilla Luciferase, which catalyses a luminescent reaction. TetR can be used to repress the promoter Ptet.
mutable notes: r
mutable provenance: BBa_R0062: Plux, BBa_B0030: Strong RBS, BBa_B0030: Strong RBS, BBa_B0010:T1 (terminator) from E. coli.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1343019_sequence/1

BBa_K1450003
type: ComponentDefinition
persistent identity: BBa K1450003
display id: BBa_K1450003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD_T7 DNA polymerase
creator: Joe Ho
created: 2014-10-08T11:00:00Z
modified: 2015-05-08T01:10:28Z
discontinued: false
dominant: false
group u list: _1828_
m user id: 0
owner id: 9774
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a T7 DNA polymerase under an arabinose promoter
mutable notes: None
mutable provenance: T7 bacteriophage
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_B0015
component: BBa_I13453
component: BBa_K1450000
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1450000
sequence annotation: BBa_B0015
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1450003_sequence/1

BBa_B0015
type: Component
persistent identity: component2411780
display id: component2411780
version: 1
top level: BBa_K1450003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_I13453
type: Component
persistent identity: component2411770
display id: component2411770
version: 1
top level: BBa_K1450003
access: public
definition: https://synbiohub.org/public/igem/BBa_I13453/1

BBa_K1450000
type: Component
persistent identity: component2411773
display id: component2411773
version: 1
top level: BBa_K1450003
access: public
definition: https://synbiohub.org/public/igem/BBa_K1450000/1

BBa_B0034
type: Component
persistent identity: component2411772
display id: component2411772
version: 1
top level: BBa_K1450003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2411772
display id: annotation2411772
version: 1
top level: BBa_K1450003
location: https://synbiohub.org/public/igem/BBa_K1450003/annotation2411772/range2411772/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1450003/annotation2411772/range2411772/1
type: Range
persistent identity: range2411772
display id: range2411772
version: 1
direction: direction/reverse
top level: BBa_K1450003
start: 139
end: 150
orientation: inline

BBa_K1450000
type: SequenceAnnotation
persistent identity: annotation2411773
display id: annotation2411773
version: 1
top level: BBa_K1450003
location: https://synbiohub.org/public/igem/BBa_K1450003/annotation2411773/range2411773/1
component: BBa_K1450000

https://synbiohub.org/public/igem/BBa_K1450003/annotation2411773/range2411773/1
type: Range
persistent identity: range2411773
display id: range2411773
version: 1
direction: direction/reverse
top level: BBa_K1450003
start: 157
end: 2274
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2411780
display id: annotation2411780
version: 1
top level: BBa_K1450003
location: https://synbiohub.org/public/igem/BBa_K1450003/annotation2411780/range2411780/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1450003/annotation2411780/range2411780/1
type: Range
persistent identity: range2411780
display id: range2411780
version: 1
direction: direction/reverse
top level: BBa_K1450003
start: 2283
end: 2411
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2411770
display id: annotation2411770
version: 1
top level: BBa_K1450003
location: https://synbiohub.org/public/igem/BBa_K1450003/annotation2411770/range2411770/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1450003/annotation2411770/range2411770/1
type: Range
persistent identity: range2411770
display id: range2411770
version: 1
direction: direction/reverse
top level: BBa_K1450003
start: 1
end: 130
orientation: inline

BBa_K1497013
type: ComponentDefinition
persistent identity: BBa K1497013
display id: BBa_K1497013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034-DFR
creator: Bastian Wagner, Christian Mende, Andreas Schmidt, Max Dombrowsky
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:45Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: a
mutable notes: a
mutable provenance: a
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1497010
component: BBa_B0034
sequence annotation: BBa_K1497010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1497013_sequence/1

BBa_K1497010
type: Component
persistent identity: component2398837
display id: component2398837
version: 1
top level: BBa_K1497013
access: public
definition: BBa_K1497010

BBa_B0034
type: Component
persistent identity: component2398836
display id: component2398836
version: 1
top level: BBa_K1497013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K1497010
type: SequenceAnnotation
persistent identity: annotation2398837
display id: annotation2398837
version: 1
top level: BBa_K1497013
location: https://synbiohub.org/public/igem/BBa_K1497013/annotation2398837/range2398837/1
component: BBa_K1497010

https://synbiohub.org/public/igem/BBa_K1497013/annotation2398837/range2398837/1
type: Range
persistent identity: range2398837
display id: range2398837
version: 1
direction: direction/reverse
top level: BBa_K1497013
start: 19
end: 1080
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2398836
display id: annotation2398836
version: 1
top level: BBa_K1497013
location: https://synbiohub.org/public/igem/BBa_K1497013/annotation2398836/range2398836/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1497013/annotation2398836/range2398836/1
type: Range
persistent identity: range2398836
display id: range2398836
version: 1
direction: direction/reverse
top level: BBa_K1497013
start: 1
end: 12
orientation: inline

BBa_K1498000
type: ComponentDefinition
persistent identity: BBa K1498000
display id: BBa_K1498000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: kerUS keratinase from Brevibacillus brevis
creator: Charlotte Green
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
group u list: _1878_
m user id: 0
owner id: 21965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region of kerUS keratinase from Brevibacillus brevis with signal for extracellular secretion, which is cleaved following secretion Codes for a 45.9kDa (predicted)pre-processed protein, cleaved to 29kDa active protein
mutable notes: codon optimisation for E. coli, ensuring no BioBrick restriction enzyme sites were present
mutable provenance: is the codon optimised (for E. coli) kerUS gene from: Jaouadi, N. Z., Rekik, H., Badis, A., Trabelsi, S., Belhoul, M., Yahiaoui, A. B., ??? Jaouadi, B. (2013). Biochemical and molecular characterization of a serine keratinase from Brevibacillus brevis US575 with promising keratin-biodegradation and hide-dehairing activities. PloS one, 8(10), e76722. doi:10.1371/journal.pone.0076722 Genbank Number: KC152966
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1498000_sequence/1

BBa_K1351012
type: ComponentDefinition
persistent identity: BBa K1351012
display id: BBa_K1351012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: spaS Subtilin (antimicrobial peptide, Freiburg standard)
creator: Carolin Kobras
created: 2014-09-09T11:00:00Z
modified: 2016-01-27T02:43:09Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1726_
m user id: 4206
owner id: 20398
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Subtilin Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
mutable notes: -
mutable provenance: Bacillus subtilis ATCC6633
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1351012_sequence/1

BBa_K1365997
type: ComponentDefinition
persistent identity: BBa K1365997
display id: BBa_K1365997
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL Sensor
creator: chandhuru Jagadeesan
created: 2014-10-07T11:00:00Z
modified: 2015-05-08T01:10:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1741_
m user id: 0
owner id: 20161
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: -----
mutable notes: ----
mutable provenance: ----
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1033222
component: BBa_B0015
component: BBa_S03156
sequence annotation: BBa_K1033222
sequence annotation: BBa_B0015
sequence annotation: BBa_S03156
sequence: https://synbiohub.org/public/igem/BBa_K1365997_sequence/1

BBa_K1033222
type: Component
persistent identity: component2401562
display id: component2401562
version: 1
top level: BBa_K1365997
access: public
definition: https://synbiohub.org/public/igem/BBa_K1033222/1

BBa_B0015
type: Component
persistent identity: component2401577
display id: component2401577
version: 1
top level: BBa_K1365997
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_S03156
type: Component
persistent identity: component2401570
display id: component2401570
version: 1
top level: BBa_K1365997
access: public
definition: https://synbiohub.org/public/igem/BBa_S03156/1

BBa_K1033222
type: SequenceAnnotation
persistent identity: annotation2401562
display id: annotation2401562
version: 1
top level: BBa_K1365997
location: https://synbiohub.org/public/igem/BBa_K1365997/annotation2401562/range2401562/1
component: BBa_K1033222

https://synbiohub.org/public/igem/BBa_K1365997/annotation2401562/range2401562/1
type: Range
persistent identity: range2401562
display id: range2401562
version: 1
direction: direction/reverse
top level: BBa_K1365997
start: 1
end: 59
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2401577
display id: annotation2401577
version: 1
top level: BBa_K1365997
location: https://synbiohub.org/public/igem/BBa_K1365997/annotation2401577/range2401577/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1365997/annotation2401577/range2401577/1
type: Range
persistent identity: range2401577
display id: range2401577
version: 1
direction: direction/reverse
top level: BBa_K1365997
start: 875
end: 1003
orientation: inline

BBa_S03156
type: SequenceAnnotation
persistent identity: annotation2401570
display id: annotation2401570
version: 1
top level: BBa_K1365997
location: https://synbiohub.org/public/igem/BBa_K1365997/annotation2401570/range2401570/1
component: BBa_S03156

https://synbiohub.org/public/igem/BBa_K1365997/annotation2401570/range2401570/1
type: Range
persistent identity: range2401570
display id: range2401570
version: 1
direction: direction/reverse
top level: BBa_K1365997
start: 68
end: 866
orientation: inline

BBa_K1321120
type: ComponentDefinition
persistent identity: BBa K1321120
display id: BBa_K1321120
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SmtA metallothionein fused to linker-dCBD
creator: Gabriella Santosa
created: 2014-10-08T11:00:00Z
modified: 2015-06-17T12:22:14Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1696_
m user id: 4206
owner id: 20961
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: GATC
mutable notes: GATC
mutable provenance: GATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1321011
component: BBa_B0105
component: BBa_K1321340
sequence annotation: BBa_B0105
sequence annotation: BBa_K1321011
sequence annotation: BBa_K1321340
sequence: https://synbiohub.org/public/igem/BBa_K1321120_sequence/1

BBa_K1321011
type: Component
persistent identity: component2414675
display id: component2414675
version: 1
top level: BBa_K1321120
access: public
definition: https://synbiohub.org/public/igem/BBa_K1321011/1

BBa_B0105
type: Component
persistent identity: component2414677
display id: component2414677
version: 1
top level: BBa_K1321120
access: public
definition: https://synbiohub.org/public/igem/BBa_B0105/1

BBa_K1321340
type: Component
persistent identity: component2414679
display id: component2414679
version: 1
top level: BBa_K1321120
access: public
definition: BBa_K1321340

BBa_B0105
type: SequenceAnnotation
persistent identity: annotation2414677
display id: annotation2414677
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414677/range2414677/1
component: BBa_B0105

https://synbiohub.org/public/igem/BBa_K1321120/annotation2414677/range2414677/1
type: Range
persistent identity: range2414677
display id: range2414677
version: 1
direction: direction/reverse
top level: BBa_K1321120
start: 166
end: 171
orientation: inline

BBa_K1321011
type: SequenceAnnotation
persistent identity: annotation2414675
display id: annotation2414675
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414675/range2414675/1
component: BBa_K1321011

https://synbiohub.org/public/igem/BBa_K1321120/annotation2414675/range2414675/1
type: Range
persistent identity: range2414675
display id: range2414675
version: 1
direction: direction/reverse
top level: BBa_K1321120
start: 1
end: 165
orientation: inline

BBa_K1321340
type: SequenceAnnotation
persistent identity: annotation2414679
display id: annotation2414679
version: 1
top level: BBa_K1321120
location: https://synbiohub.org/public/igem/BBa_K1321120/annotation2414679/range2414679/1
component: BBa_K1321340

https://synbiohub.org/public/igem/BBa_K1321120/annotation2414679/range2414679/1
type: Range
persistent identity: range2414679
display id: range2414679
version: 1
direction: direction/reverse
top level: BBa_K1321120
start: 172
end: 537
orientation: inline

BBa_K1497030
type: ComponentDefinition
persistent identity: BBa K1497030
display id: BBa_K1497030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Scaffold (S-S)
creator: Tobias Gabriel, Rene Sahm
created: 2014-10-06T11:00:00Z
modified: 2015-05-08T01:10:46Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1877_
m user id: 0
owner id: 12466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Scaffold (S-S)
mutable notes: Scaffold (S-S)
mutable provenance: Scaffold (S-S)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1497030_sequence/1

BBa_K1415103
type: ComponentDefinition
persistent identity: BBa K1415103
display id: BBa_K1415103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pcons+B0034+PBAN(Agrotis ipsilon)
creator: HO, TSUNG YU
created: 2014-10-04T11:00:00Z
modified: 2015-05-08T01:10:19Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1793_
m user id: 0
owner id: 22512
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has a constitutive promoter, so our PBAN(Agrotis ipsilon) can constitutive express.
mutable notes: No
mutable provenance: Assemble
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1415003
component: BBa_B0034
component: BBa_J23101
sequence annotation: BBa_K1415003
sequence annotation: BBa_J23101
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K1415103_sequence/1

BBa_K1415003
type: Component
persistent identity: component2393969
display id: component2393969
version: 1
top level: BBa_K1415103
access: public
definition: BBa_K1415003

BBa_B0034
type: Component
persistent identity: component2393967
display id: component2393967
version: 1
top level: BBa_K1415103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23101
type: Component
persistent identity: component2393965
display id: component2393965
version: 1
top level: BBa_K1415103
access: public
definition: https://synbiohub.org/public/igem/BBa_J23101/1

BBa_K1415003
type: SequenceAnnotation
persistent identity: annotation2393969
display id: annotation2393969
version: 1
top level: BBa_K1415103
location: https://synbiohub.org/public/igem/BBa_K1415103/annotation2393969/range2393969/1
component: BBa_K1415003

https://synbiohub.org/public/igem/BBa_K1415103/annotation2393969/range2393969/1
type: Range
persistent identity: range2393969
display id: range2393969
version: 1
direction: direction/reverse
top level: BBa_K1415103
start: 62
end: 170
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2393965
display id: annotation2393965
version: 1
top level: BBa_K1415103
location: https://synbiohub.org/public/igem/BBa_K1415103/annotation2393965/range2393965/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1415103/annotation2393965/range2393965/1
type: Range
persistent identity: range2393965
display id: range2393965
version: 1
direction: direction/reverse
top level: BBa_K1415103
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2393967
display id: annotation2393967
version: 1
top level: BBa_K1415103
location: https://synbiohub.org/public/igem/BBa_K1415103/annotation2393967/range2393967/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1415103/annotation2393967/range2393967/1
type: Range
persistent identity: range2393967
display id: range2393967
version: 1
direction: direction/reverse
top level: BBa_K1415103
start: 44
end: 55
orientation: inline

BBa_K1420005
type: ComponentDefinition
persistent identity: BBa K1420005
display id: BBa_K1420005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: merT, mercuric transport protein
creator: Stephen C. Heinsch
created: 2014-10-08T11:00:00Z
modified: 2015-07-22T03:01:03Z
discontinued: false
dominant: false
group u list: _1798_
m user id: 4206
owner id: 17872
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: merT is found in the Serratia marcescens plasmid pDU1358 on the other side of the bidirectional promoter opposite of merR. It is 351 base pairs long and is hypothesized that it codes for a mercuric transport protein.
mutable notes: N/A
mutable provenance: Source organism: Serratia marcescens. Amplified from plasmid pDU1358, kindly donated by Anne O. Summers.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: merT
sequence: https://synbiohub.org/public/igem/BBa_K1420005_sequence/1

merT
type: SequenceAnnotation
persistent identity: annotation2407640
display id: annotation2407640
version: 1
top level: BBa_K1420005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1420005/annotation2407640/range2407640/1

https://synbiohub.org/public/igem/BBa_K1420005/annotation2407640/range2407640/1
type: Range
persistent identity: range2407640
display id: range2407640
version: 1
direction: direction/forward
top level: BBa_K1420005
start: 1
end: 351
orientation: inline

BBa_K1325005
type: ComponentDefinition
persistent identity: BBa K1325005
display id: BBa_K1325005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MinC??????cell division inhibitor
creator: Ke Xu
created: 2014-09-28T11:00:00Z
modified: 2015-05-08T01:09:54Z
discontinued: false
dominant: false
group u list: _1700_
m user id: 0
owner id: 23253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: cell division inhibitor of the MinC-MinD-MinE and DicB-MinC systems that regulate septum placement
mutable notes: no
mutable provenance: E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1325005_sequence/1

BBa_K1404007
type: ComponentDefinition
persistent identity: BBa K1404007
display id: BBa_K1404007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: p70-CsgA-His, His-tagged curli generator
creator: Juliette Paillet, Alexandre Duprey, Emiel van der Kouwe
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:10:17Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1782_
m user id: 0
owner id: 21195
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: temp
mutable notes: temp
mutable provenance: temp
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CsgG signal
sequence annotation: Linker
sequence annotation: CsgA-His1
sequence annotation: Beta sheet4
sequence annotation: Beta sheet 1
sequence annotation: His tag
sequence annotation: Beta sheet5
sequence annotation: Sec signal
sequence annotation: RBS Paris 2009
sequence annotation: Beta sheet3
sequence annotation: BBa_K342000
sequence annotation: GCT into GCC
sequence annotation: Beta sheet2
sequence: https://synbiohub.org/public/igem/BBa_K1404007_sequence/1

CsgG signal
type: SequenceAnnotation
persistent identity: annotation2426222
display id: annotation2426222
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426222/range2426222/1
type: Range
persistent identity: range2426222
display id: range2426222
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 152
end: 217
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2426229
display id: annotation2426229
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426229/range2426229/1
type: Range
persistent identity: range2426229
display id: range2426229
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 545
end: 556
orientation: inline

CsgA-His1
type: SequenceAnnotation
persistent identity: annotation2426219
display id: annotation2426219
version: 1
top level: BBa_K1404007
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426219/range2426219/1
type: Range
persistent identity: range2426219
display id: range2426219
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 577
orientation: inline

Beta sheet4
type: SequenceAnnotation
persistent identity: annotation2426226
display id: annotation2426226
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426226/range2426226/1
type: Range
persistent identity: range2426226
display id: range2426226
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 422
end: 487
orientation: inline

Beta sheet 1
type: SequenceAnnotation
persistent identity: annotation2426223
display id: annotation2426223
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426223/range2426223/1
type: Range
persistent identity: range2426223
display id: range2426223
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 218
end: 286
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2426230
display id: annotation2426230
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426230/range2426230/1
type: Range
persistent identity: range2426230
display id: range2426230
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 557
end: 574
orientation: inline

Beta sheet5
type: SequenceAnnotation
persistent identity: annotation2426228
display id: annotation2426228
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426228/range2426228/1
type: Range
persistent identity: range2426228
display id: range2426228
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 488
end: 544
orientation: inline

Sec signal
type: SequenceAnnotation
persistent identity: annotation2426221
display id: annotation2426221
version: 1
top level: BBa_K1404007
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426221/range2426221/1
type: Range
persistent identity: range2426221
display id: range2426221
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 92
end: 151
orientation: inline

RBS Paris 2009
type: SequenceAnnotation
persistent identity: annotation2426220
display id: annotation2426220
version: 1
top level: BBa_K1404007
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426220/range2426220/1
type: Range
persistent identity: range2426220
display id: range2426220
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 79
end: 91
orientation: inline

Beta sheet3
type: SequenceAnnotation
persistent identity: annotation2426225
display id: annotation2426225
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426225/range2426225/1
type: Range
persistent identity: range2426225
display id: range2426225
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 353
end: 421
orientation: inline

BBa_K342000
type: SequenceAnnotation
persistent identity: annotation2426218
display id: annotation2426218
version: 1
top level: BBa_K1404007
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426218/range2426218/1
type: Range
persistent identity: range2426218
display id: range2426218
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 1
end: 70
orientation: inline

GCT into GCC
type: SequenceAnnotation
persistent identity: annotation2426227
display id: annotation2426227
version: 1
top level: BBa_K1404007
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426227/range2426227/1
type: Range
persistent identity: range2426227
display id: range2426227
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 461
end: 463
orientation: inline

Beta sheet2
type: SequenceAnnotation
persistent identity: annotation2426224
display id: annotation2426224
version: 1
top level: BBa_K1404007
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1

https://synbiohub.org/public/igem/BBa_K1404007/annotation2426224/range2426224/1
type: Range
persistent identity: range2426224
display id: range2426224
version: 1
direction: direction/forward
top level: BBa_K1404007
start: 287
end: 352
orientation: inline

ComE
type: ComponentDefinition
persistent identity: BBa K1379051
display id: BBa_K1379051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S. pneumoniae ComE CDS
creator: Jacqueline Theeng Mei Aw
created: 2014-10-13T11:00:00Z
modified: 2015-05-08T01:10:13Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1756_
m user id: 0
owner id: 21304
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ComE is part of a two-component regulatory system (TCS) that controls competence development in Streptococcus pneumoniae. ComE is the response regulator to histidine kinase ComD. Upon binding and detection of the quorum-sensing peptide CSP, transmembrane receptor ComD autophosphorylates using ATP. Subsequently, cytosolic protein ComE is transphosphorylated by ComD. ComE~P activates the comCDE and comAB operons for expression of late competence genes.
mutable notes: Removal of SpeI illegal site
mutable provenance: S. pneumoniae strain NCTC7465
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: comE
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1379051_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation2418764
display id: annotation2418764
version: 1
top level: ComE
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1379051/annotation2418764/range2418764/1

https://synbiohub.org/public/igem/BBa_K1379051/annotation2418764/range2418764/1
type: Range
persistent identity: range2418764
display id: range2418764
version: 1
direction: direction/forward
top level: ComE
start: 1
end: 3
orientation: inline

comE
type: SequenceAnnotation
persistent identity: annotation2418766
display id: annotation2418766
version: 1
top level: ComE
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1379051/annotation2418766/range2418766/1

https://synbiohub.org/public/igem/BBa_K1379051/annotation2418766/range2418766/1
type: Range
persistent identity: range2418766
display id: range2418766
version: 1
direction: direction/forward
top level: ComE
start: 1
end: 753
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2418765
display id: annotation2418765
version: 1
top level: ComE
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1379051/annotation2418765/range2418765/1

https://synbiohub.org/public/igem/BBa_K1379051/annotation2418765/range2418765/1
type: Range
persistent identity: range2418765
display id: range2418765
version: 1
direction: direction/forward
top level: ComE
start: 748
end: 753
orientation: inline

BBa_K1505000
type: ComponentDefinition
persistent identity: BBa K1505000
display id: BBa_K1505000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS+mntH
creator: Renyao Wei
created: 2014-10-08T11:00:00Z
modified: 2016-01-13T10:32:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1885_
m user id: 4206
owner id: 22465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The gene was functionally expressed in Azospirillum sp. B510. It encodes a divalent metal cation transporter. The protein can be used to increase transportation level of divalent metal ions into the cells. Its potential applications include heavy metal bioremediation (by increasing uptake efficiency) and protein expression in cell surface (by fusion with mntH).
mutable notes: It is the coding sequence of MntH
mutable provenance: Azospirillum sp. B510 genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs (from pmsp3)
sequence annotation: mntH
sequence: https://synbiohub.org/public/igem/BBa_K1505000_sequence/1

rbs (from pmsp3)
type: SequenceAnnotation
persistent identity: annotation2412322
display id: annotation2412322
version: 1
top level: BBa_K1505000
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1505000/annotation2412322/range2412322/1

https://synbiohub.org/public/igem/BBa_K1505000/annotation2412322/range2412322/1
type: Range
persistent identity: range2412322
display id: range2412322
version: 1
direction: direction/forward
top level: BBa_K1505000
start: 19
end: 46
orientation: inline

mntH
type: SequenceAnnotation
persistent identity: annotation2412323
display id: annotation2412323
version: 1
top level: BBa_K1505000
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K1505000/annotation2412323/range2412323/1

https://synbiohub.org/public/igem/BBa_K1505000/annotation2412323/range2412323/1
type: Range
persistent identity: range2412323
display id: range2412323
version: 1
direction: direction/forward
top level: BBa_K1505000
start: 67
end: 1302
orientation: inline