iGEM 2018 Distribution Plate 4 Well 6O
type: Collection
persistent identity: iGEM 2018 Plate4 Well6O
display id: iGEM_2018_Plate4_Well6O
version: 1
owned by: myers
member: BBa_K1150001

iGEM 2018 Distribution Plate 4 Well 15B
type: Collection
persistent identity: iGEM 2018 Plate4 Well15B
display id: iGEM_2018_Plate4_Well15B
version: 1
owned by: myers
member: EYFP

iGEM 2018 Distribution Plate 4 Well 3J
type: Collection
persistent identity: iGEM 2018 Plate4 Well3J
display id: iGEM_2018_Plate4_Well3J
version: 1
owned by: myers
member: c2 P22

iGEM 2018 Distribution Plate 4 Well 23B
type: Collection
persistent identity: iGEM 2018 Plate4 Well23B
display id: iGEM_2018_Plate4_Well23B
version: 1
owned by: myers
member: BBa_C0056

iGEM 2018 Distribution Plate 4 Well 16A
type: Collection
persistent identity: iGEM 2018 Plate4 Well16A
display id: iGEM_2018_Plate4_Well16A
version: 1
owned by: myers
member: BBa_B0025

iGEM 2018 Distribution Plate 4 Well 1G
type: Collection
persistent identity: iGEM 2018 Plate4 Well1G
display id: iGEM_2018_Plate4_Well1G
version: 1
owned by: myers
member: BBa_K145302

iGEM 2018 Distribution Plate 4 Well 12D
type: Collection
persistent identity: iGEM 2018 Plate4 Well12D
display id: iGEM_2018_Plate4_Well12D
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 2F
type: Collection
persistent identity: iGEM 2018 Plate4 Well2F
display id: iGEM_2018_Plate4_Well2F
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 15C
type: Collection
persistent identity: iGEM 2018 Plate4 Well15C
display id: iGEM_2018_Plate4_Well15C
version: 1
owned by: myers
member: BBa_K115002

iGEM 2018 Distribution Plate 4 Well 19F
type: Collection
persistent identity: iGEM 2018 Plate4 Well19F
display id: iGEM_2018_Plate4_Well19F
version: 1
owned by: myers
member: BBa_J23112

iGEM 2018 Distribution Plate 4 Well 15F
type: Collection
persistent identity: iGEM 2018 Plate4 Well15F
display id: iGEM_2018_Plate4_Well15F
version: 1
owned by: myers
member: OmpR

iGEM 2018 Distribution Plate 4 Well 5O
type: Collection
persistent identity: iGEM 2018 Plate4 Well5O
display id: iGEM_2018_Plate4_Well5O
version: 1
owned by: myers
member: BBa_I6057

iGEM 2018 Distribution Plate 4 Well 5D
type: Collection
persistent identity: iGEM 2018 Plate4 Well5D
display id: iGEM_2018_Plate4_Well5D
version: 1
owned by: myers
member: ECFP

iGEM 2018 Distribution Plate 4 Well 8C
type: Collection
persistent identity: iGEM 2018 Plate4 Well8C
display id: iGEM_2018_Plate4_Well8C
version: 1
owned by: myers
member: BBa_K1150003

iGEM 2018 Distribution Plate 4 Well 12M
type: Collection
persistent identity: iGEM 2018 Plate4 Well12M
display id: iGEM_2018_Plate4_Well12M
version: 1
owned by: myers
member: BBa_K1149051

iGEM 2018 Distribution Plate 4 Well 5P
type: Collection
persistent identity: iGEM 2018 Plate4 Well5P
display id: iGEM_2018_Plate4_Well5P
version: 1
owned by: myers
member: lux pR

iGEM 2018 Distribution Plate 4 Well 1L
type: Collection
persistent identity: iGEM 2018 Plate4 Well1L
display id: iGEM_2018_Plate4_Well1L
version: 1
owned by: myers
member: BBa_B0033

iGEM 2018 Distribution Plate 4 Well 11F
type: Collection
persistent identity: iGEM 2018 Plate4 Well11F
display id: iGEM_2018_Plate4_Well11F
version: 1
owned by: myers
member: AraC

iGEM 2018 Distribution Plate 4 Well 2G
type: Collection
persistent identity: iGEM 2018 Plate4 Well2G
display id: iGEM_2018_Plate4_Well2G
version: 1
owned by: myers
member: SbpA | CFP

iGEM 2018 Distribution Plate 4 Well 7O
type: Collection
persistent identity: iGEM 2018 Plate4 Well7O
display id: iGEM_2018_Plate4_Well7O
version: 1
owned by: myers
member: BBa_I759020

iGEM 2018 Distribution Plate 4 Well 6I
type: Collection
persistent identity: iGEM 2018 Plate4 Well6I
display id: iGEM_2018_Plate4_Well6I
version: 1
owned by: myers
member: BBa_K1166002

iGEM 2018 Distribution Plate 4 Well 12B
type: Collection
persistent identity: iGEM 2018 Plate4 Well12B
display id: iGEM_2018_Plate4_Well12B
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 2D
type: Collection
persistent identity: iGEM 2018 Plate4 Well2D
display id: iGEM_2018_Plate4_Well2D
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 23D
type: Collection
persistent identity: iGEM 2018 Plate4 Well23D
display id: iGEM_2018_Plate4_Well23D
version: 1
owned by: myers
member: spacer

iGEM 2018 Distribution Plate 4 Well 15A
type: Collection
persistent identity: iGEM 2018 Plate4 Well15A
display id: iGEM_2018_Plate4_Well15A
version: 1
owned by: myers
member: BBa_K115001

iGEM 2018 Distribution Plate 4 Well 23N
type: Collection
persistent identity: iGEM 2018 Plate4 Well23N
display id: iGEM_2018_Plate4_Well23N
version: 1
owned by: myers
member: CmR

iGEM 2018 Distribution Plate 4 Well 4O
type: Collection
persistent identity: iGEM 2018 Plate4 Well4O
display id: iGEM_2018_Plate4_Well4O
version: 1
owned by: myers
member: BBa_K1184000

iGEM 2018 Distribution Plate 4 Well 6A
type: Collection
persistent identity: iGEM 2018 Plate4 Well6A
display id: iGEM_2018_Plate4_Well6A
version: 1
owned by: myers
member: BBa_K1031410

iGEM 2018 Distribution Plate 4 Well 17F
type: Collection
persistent identity: iGEM 2018 Plate4 Well17F
display id: iGEM_2018_Plate4_Well17F
version: 1
owned by: myers
member: BBa_J23101

iGEM 2018 Distribution Plate 4 Well 1J
type: Collection
persistent identity: iGEM 2018 Plate4 Well1J
display id: iGEM_2018_Plate4_Well1J
version: 1
owned by: myers
member: BBa_B0032

iGEM 2018 Distribution Plate 4 Well 9L
type: Collection
persistent identity: iGEM 2018 Plate4 Well9L
display id: iGEM_2018_Plate4_Well9L
version: 1
owned by: myers
member: cinR

iGEM 2018 Distribution Plate 4 Well 2O
type: Collection
persistent identity: iGEM 2018 Plate4 Well2O
display id: iGEM_2018_Plate4_Well2O
version: 1
owned by: myers
member: BBa_K648013

iGEM 2018 Distribution Plate 4 Well 3L
type: Collection
persistent identity: iGEM 2018 Plate4 Well3L
display id: iGEM_2018_Plate4_Well3L
version: 1
owned by: myers
member: aiiA

iGEM 2018 Distribution Plate 4 Well 3G
type: Collection
persistent identity: iGEM 2018 Plate4 Well3G
display id: iGEM_2018_Plate4_Well3G
version: 1
owned by: myers
member: BBa_K118028

iGEM 2018 Distribution Plate 4 Well 11B
type: Collection
persistent identity: iGEM 2018 Plate4 Well11B
display id: iGEM_2018_Plate4_Well11B
version: 1
owned by: myers
member: LasR+PAI

iGEM 2018 Distribution Plate 4 Well 3F
type: Collection
persistent identity: iGEM 2018 Plate4 Well3F
display id: iGEM_2018_Plate4_Well3F
version: 1
owned by: myers
member: cI lam

iGEM 2018 Distribution Plate 4 Well 11C
type: Collection
persistent identity: iGEM 2018 Plate4 Well11C
display id: iGEM_2018_Plate4_Well11C
version: 1
owned by: myers
member: BBa_J52039

iGEM 2018 Distribution Plate 4 Well 7N
type: Collection
persistent identity: iGEM 2018 Plate4 Well7N
display id: iGEM_2018_Plate4_Well7N
version: 1
owned by: myers
member: BBa_E0422

iGEM 2018 Distribution Plate 4 Well 15P
type: Collection
persistent identity: iGEM 2018 Plate4 Well15P
display id: iGEM_2018_Plate4_Well15P
version: 1
owned by: myers
member: BBa_J23070

iGEM 2018 Distribution Plate 4 Well 10G
type: Collection
persistent identity: iGEM 2018 Plate4 Well10G
display id: iGEM_2018_Plate4_Well10G
version: 1
owned by: myers
member: BBa_K1150016

iGEM 2018 Distribution Plate 4 Well 23F
type: Collection
persistent identity: iGEM 2018 Plate4 Well23F
display id: iGEM_2018_Plate4_Well23F
version: 1
owned by: myers
member: BBa_I13600

iGEM 2018 Distribution Plate 4 Well 4M
type: Collection
persistent identity: iGEM 2018 Plate4 Well4M
display id: iGEM_2018_Plate4_Well4M
version: 1
owned by: myers
member: BBa_K1218011

iGEM 2018 Distribution Plate 4 Well 7M
type: Collection
persistent identity: iGEM 2018 Plate4 Well7M
display id: iGEM_2018_Plate4_Well7M
version: 1
owned by: myers
member: BBa_I759019

iGEM 2018 Distribution Plate 4 Well 10B
type: Collection
persistent identity: iGEM 2018 Plate4 Well10B
display id: iGEM_2018_Plate4_Well10B
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 4F
type: Collection
persistent identity: iGEM 2018 Plate4 Well4F
display id: iGEM_2018_Plate4_Well4F
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 2B
type: Collection
persistent identity: iGEM 2018 Plate4 Well2B
display id: iGEM_2018_Plate4_Well2B
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 11O
type: Collection
persistent identity: iGEM 2018 Plate4 Well11O
display id: iGEM_2018_Plate4_Well11O
version: 1
owned by: myers
member: BBa_I746200

iGEM 2018 Distribution Plate 4 Well 5E
type: Collection
persistent identity: iGEM 2018 Plate4 Well5E
display id: iGEM_2018_Plate4_Well5E
version: 1
owned by: myers
member: BBa_K137076

iGEM 2018 Distribution Plate 4 Well 10A
type: Collection
persistent identity: iGEM 2018 Plate4 Well10A
display id: iGEM_2018_Plate4_Well10A
version: 1
owned by: myers
member: BBa_K1150012

iGEM 2018 Distribution Plate 4 Well 19H
type: Collection
persistent identity: iGEM 2018 Plate4 Well19H
display id: iGEM_2018_Plate4_Well19H
version: 1
owned by: myers
member: BBa_J23113

iGEM 2018 Distribution Plate 4 Well 21F
type: Collection
persistent identity: iGEM 2018 Plate4 Well21F
display id: iGEM_2018_Plate4_Well21F
version: 1
owned by: myers
member: BBa_I13500

iGEM 2018 Distribution Plate 4 Well 3K
type: Collection
persistent identity: iGEM 2018 Plate4 Well3K
display id: iGEM_2018_Plate4_Well3K
version: 1
owned by: myers
member: BBa_I746375

iGEM 2018 Distribution Plate 4 Well 17B
type: Collection
persistent identity: iGEM 2018 Plate4 Well17B
display id: iGEM_2018_Plate4_Well17B
version: 1
owned by: myers
member: BBa_J23119

iGEM 2018 Distribution Plate 4 Well 7B
type: Collection
persistent identity: iGEM 2018 Plate4 Well7B
display id: iGEM_2018_Plate4_Well7B
version: 1
owned by: myers
member: lux pL

iGEM 2018 Distribution Plate 4 Well 1B
type: Collection
persistent identity: iGEM 2018 Plate4 Well1B
display id: iGEM_2018_Plate4_Well1B
version: 1
owned by: myers
member: BBa_B0011

iGEM 2018 Distribution Plate 4 Well 1F
type: Collection
persistent identity: iGEM 2018 Plate4 Well1F
display id: iGEM_2018_Plate4_Well1F
version: 1
owned by: myers
member: BBa_B0025

iGEM 2018 Distribution Plate 4 Well 1N
type: Collection
persistent identity: iGEM 2018 Plate4 Well1N
display id: iGEM_2018_Plate4_Well1N
version: 1
owned by: myers
member: BBa_B0034

iGEM 2018 Distribution Plate 4 Well 17D
type: Collection
persistent identity: iGEM 2018 Plate4 Well17D
display id: iGEM_2018_Plate4_Well17D
version: 1
owned by: myers
member: BBa_J23100

iGEM 2018 Distribution Plate 4 Well 3H
type: Collection
persistent identity: iGEM 2018 Plate4 Well3H
display id: iGEM_2018_Plate4_Well3H
version: 1
owned by: myers
member: cI 434

iGEM 2018 Distribution Plate 4 Well 20A
type: Collection
persistent identity: iGEM 2018 Plate4 Well20A
display id: iGEM_2018_Plate4_Well20A
version: 1
owned by: myers
member: BBa_J52026

iGEM 2018 Distribution Plate 4 Well 2M
type: Collection
persistent identity: iGEM 2018 Plate4 Well2M
display id: iGEM_2018_Plate4_Well2M
version: 1
owned by: myers
member: YFP

iGEM 2018 Distribution Plate 4 Well 2J
type: Collection
persistent identity: iGEM 2018 Plate4 Well2J
display id: iGEM_2018_Plate4_Well2J
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 10I
type: Collection
persistent identity: iGEM 2018 Plate4 Well10I
display id: iGEM_2018_Plate4_Well10I
version: 1
owned by: myers
member: RNAimer

iGEM 2018 Distribution Plate 4 Well 9P
type: Collection
persistent identity: iGEM 2018 Plate4 Well9P
display id: iGEM_2018_Plate4_Well9P
version: 1
owned by: myers
member: BBa_S03119

iGEM 2018 Distribution Plate 4 Well 8A
type: Collection
persistent identity: iGEM 2018 Plate4 Well8A
display id: iGEM_2018_Plate4_Well8A
version: 1
owned by: myers
member: BBa_K1150002

iGEM 2018 Distribution Plate 4 Well 9E
type: Collection
persistent identity: iGEM 2018 Plate4 Well9E
display id: iGEM_2018_Plate4_Well9E
version: 1
owned by: myers
member: BBa_I759027

iGEM 2018 Distribution Plate 4 Well 19N
type: Collection
persistent identity: iGEM 2018 Plate4 Well19N
display id: iGEM_2018_Plate4_Well19N
version: 1
owned by: myers
member: BBa_J23116

iGEM 2018 Distribution Plate 4 Well 6H
type: Collection
persistent identity: iGEM 2018 Plate4 Well6H
display id: iGEM_2018_Plate4_Well6H
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 18E
type: Collection
persistent identity: iGEM 2018 Plate4 Well18E
display id: iGEM_2018_Plate4_Well18E
version: 1
owned by: myers
member: GFP report

iGEM 2018 Distribution Plate 4 Well 4D
type: Collection
persistent identity: iGEM 2018 Plate4 Well4D
display id: iGEM_2018_Plate4_Well4D
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 3P
type: Collection
persistent identity: iGEM 2018 Plate4 Well3P
display id: iGEM_2018_Plate4_Well3P
version: 1
owned by: myers
member: luxr

iGEM 2018 Distribution Plate 4 Well 14B
type: Collection
persistent identity: iGEM 2018 Plate4 Well14B
display id: iGEM_2018_Plate4_Well14B
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 1E
type: Collection
persistent identity: iGEM 2018 Plate4 Well1E
display id: iGEM_2018_Plate4_Well1E
version: 1
owned by: myers
member: T7

iGEM 2018 Distribution Plate 4 Well 10C
type: Collection
persistent identity: iGEM 2018 Plate4 Well10C
display id: iGEM_2018_Plate4_Well10C
version: 1
owned by: myers
member: BBa_K1150012

iGEM 2018 Distribution Plate 4 Well 13J
type: Collection
persistent identity: iGEM 2018 Plate4 Well13J
display id: iGEM_2018_Plate4_Well13J
version: 1
owned by: myers
member: EYFP

iGEM 2018 Distribution Plate 4 Well 2I
type: Collection
persistent identity: iGEM 2018 Plate4 Well2I
display id: iGEM_2018_Plate4_Well2I
version: 1
owned by: myers
member: cjBlue

iGEM 2018 Distribution Plate 4 Well 7K
type: Collection
persistent identity: iGEM 2018 Plate4 Well7K
display id: iGEM_2018_Plate4_Well7K
version: 1
owned by: myers
member: BBa_I759018

iGEM 2018 Distribution Plate 4 Well 12A
type: Collection
persistent identity: iGEM 2018 Plate4 Well12A
display id: iGEM_2018_Plate4_Well12A
version: 1
owned by: myers
member: BBa_K1150038

iGEM 2018 Distribution Plate 4 Well 1P
type: Collection
persistent identity: iGEM 2018 Plate4 Well1P
display id: iGEM_2018_Plate4_Well1P
version: 1
owned by: myers
member: lacI

iGEM 2018 Distribution Plate 4 Well 4I
type: Collection
persistent identity: iGEM 2018 Plate4 Well4I
display id: iGEM_2018_Plate4_Well4I
version: 1
owned by: myers
member: BBa_K1188002

iGEM 2018 Distribution Plate 4 Well 7A
type: Collection
persistent identity: iGEM 2018 Plate4 Well7A
display id: iGEM_2018_Plate4_Well7A
version: 1
owned by: myers
member: BBa_J52011

iGEM 2018 Distribution Plate 4 Well 13F
type: Collection
persistent identity: iGEM 2018 Plate4 Well13F
display id: iGEM_2018_Plate4_Well13F
version: 1
owned by: myers
member: BBa_S03154

iGEM 2018 Distribution Plate 4 Well 8B
type: Collection
persistent identity: iGEM 2018 Plate4 Well8B
display id: iGEM_2018_Plate4_Well8B
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 9H
type: Collection
persistent identity: iGEM 2018 Plate4 Well9H
display id: iGEM_2018_Plate4_Well9H
version: 1
owned by: myers
member: BBa_P0153

iGEM 2018 Distribution Plate 4 Well 3M
type: Collection
persistent identity: iGEM 2018 Plate4 Well3M
display id: iGEM_2018_Plate4_Well3M
version: 1
owned by: myers
member: BBa_I746381

iGEM 2018 Distribution Plate 4 Well 15H
type: Collection
persistent identity: iGEM 2018 Plate4 Well15H
display id: iGEM_2018_Plate4_Well15H
version: 1
owned by: myers
member: cinR

iGEM 2018 Distribution Plate 4 Well 19P
type: Collection
persistent identity: iGEM 2018 Plate4 Well19P
display id: iGEM_2018_Plate4_Well19P
version: 1
owned by: myers
member: BBa_J23117

iGEM 2018 Distribution Plate 4 Well 13P
type: Collection
persistent identity: iGEM 2018 Plate4 Well13P
display id: iGEM_2018_Plate4_Well13P
version: 1
owned by: myers
member: ECFP

iGEM 2018 Distribution Plate 4 Well 9M
type: Collection
persistent identity: iGEM 2018 Plate4 Well9M
display id: iGEM_2018_Plate4_Well9M
version: 1
owned by: myers
member: BBa_J52038

iGEM 2018 Distribution Plate 4 Well 9J
type: Collection
persistent identity: iGEM 2018 Plate4 Well9J
display id: iGEM_2018_Plate4_Well9J
version: 1
owned by: myers
member: BBa_P0440

iGEM 2018 Distribution Plate 4 Well 16K
type: Collection
persistent identity: iGEM 2018 Plate4 Well16K
display id: iGEM_2018_Plate4_Well16K
version: 1
owned by: myers
member: BBa_K125500

iGEM 2018 Distribution Plate 4 Well 3N
type: Collection
persistent identity: iGEM 2018 Plate4 Well3N
display id: iGEM_2018_Plate4_Well3N
version: 1
owned by: myers
member: luxI

iGEM 2018 Distribution Plate 4 Well 19D
type: Collection
persistent identity: iGEM 2018 Plate4 Well19D
display id: iGEM_2018_Plate4_Well19D
version: 1
owned by: myers
member: BBa_J23110

iGEM 2018 Distribution Plate 4 Well 1I
type: Collection
persistent identity: iGEM 2018 Plate4 Well1I
display id: iGEM_2018_Plate4_Well1I
version: 1
owned by: myers
member: BBa_K145280

iGEM 2018 Distribution Plate 4 Well 9O
type: Collection
persistent identity: iGEM 2018 Plate4 Well9O
display id: iGEM_2018_Plate4_Well9O
version: 1
owned by: myers
member: BBa_J52029

iGEM 2018 Distribution Plate 4 Well 3C
type: Collection
persistent identity: iGEM 2018 Plate4 Well3C
display id: iGEM_2018_Plate4_Well3C
version: 1
owned by: myers
member: BBa_K118019

iGEM 2018 Distribution Plate 4 Well 9F
type: Collection
persistent identity: iGEM 2018 Plate4 Well9F
display id: iGEM_2018_Plate4_Well9F
version: 1
owned by: myers
member: BBa_P0152

iGEM 2018 Distribution Plate 4 Well 10E
type: Collection
persistent identity: iGEM 2018 Plate4 Well10E
display id: iGEM_2018_Plate4_Well10E
version: 1
owned by: myers
member: BBa_K1150013

iGEM 2018 Distribution Plate 4 Well 11E
type: Collection
persistent identity: iGEM 2018 Plate4 Well11E
display id: iGEM_2018_Plate4_Well11E
version: 1
owned by: myers
member: BBa_J52022

iGEM 2018 Distribution Plate 4 Well 2L
type: Collection
persistent identity: iGEM 2018 Plate4 Well2L
display id: iGEM_2018_Plate4_Well2L
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 9K
type: Collection
persistent identity: iGEM 2018 Plate4 Well9K
display id: iGEM_2018_Plate4_Well9K
version: 1
owned by: myers
member: BBa_P0312

iGEM 2018 Distribution Plate 4 Well 16E
type: Collection
persistent identity: iGEM 2018 Plate4 Well16E
display id: iGEM_2018_Plate4_Well16E
version: 1
owned by: myers
member: BBa_J61101

iGEM 2018 Distribution Plate 4 Well 15O
type: Collection
persistent identity: iGEM 2018 Plate4 Well15O
display id: iGEM_2018_Plate4_Well15O
version: 1
owned by: myers
member: BBa_K747095

iGEM 2018 Distribution Plate 4 Well 3E
type: Collection
persistent identity: iGEM 2018 Plate4 Well3E
display id: iGEM_2018_Plate4_Well3E
version: 1
owned by: myers
member: BBa_K118024

iGEM 2018 Distribution Plate 4 Well 19J
type: Collection
persistent identity: iGEM 2018 Plate4 Well19J
display id: iGEM_2018_Plate4_Well19J
version: 1
owned by: myers
member: BBa_J23114

iGEM 2018 Distribution Plate 4 Well 4K
type: Collection
persistent identity: iGEM 2018 Plate4 Well4K
display id: iGEM_2018_Plate4_Well4K
version: 1
owned by: myers
member: Cse1

iGEM 2018 Distribution Plate 4 Well 5K
type: Collection
persistent identity: iGEM 2018 Plate4 Well5K
display id: iGEM_2018_Plate4_Well5K
version: 1
owned by: myers
member: KaiA_KaiC_

iGEM 2018 Distribution Plate 4 Well 9A
type: Collection
persistent identity: iGEM 2018 Plate4 Well9A
display id: iGEM_2018_Plate4_Well9A
version: 1
owned by: myers
member: BBa_I759009

iGEM 2018 Distribution Plate 4 Well 9I
type: Collection
persistent identity: iGEM 2018 Plate4 Well9I
display id: iGEM_2018_Plate4_Well9I
version: 1
owned by: myers
member: BBa_I751102

iGEM 2018 Distribution Plate 4 Well 21D
type: Collection
persistent identity: iGEM 2018 Plate4 Well21D
display id: iGEM_2018_Plate4_Well21D
version: 1
owned by: myers
member: BBa_I13452

iGEM 2018 Distribution Plate 4 Well 15G
type: Collection
persistent identity: iGEM 2018 Plate4 Well15G
display id: iGEM_2018_Plate4_Well15G
version: 1
owned by: myers
member: BBa_K115016

iGEM 2018 Distribution Plate 4 Well 7G
type: Collection
persistent identity: iGEM 2018 Plate4 Well7G
display id: iGEM_2018_Plate4_Well7G
version: 1
owned by: myers
member: BBa_I759016

iGEM 2018 Distribution Plate 4 Well 9G
type: Collection
persistent identity: iGEM 2018 Plate4 Well9G
display id: iGEM_2018_Plate4_Well9G
version: 1
owned by: myers
member: BBa_I751101

iGEM 2018 Distribution Plate 4 Well 13D
type: Collection
persistent identity: iGEM 2018 Plate4 Well13D
display id: iGEM_2018_Plate4_Well13D
version: 1
owned by: myers
member: BBa_C0261

iGEM 2018 Distribution Plate 4 Well 15E
type: Collection
persistent identity: iGEM 2018 Plate4 Well15E
display id: iGEM_2018_Plate4_Well15E
version: 1
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member: BBa_K115003

iGEM 2018 Distribution Plate 4 Well 13N
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persistent identity: iGEM 2018 Plate4 Well13N
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version: 1
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member: ECFP

iGEM 2018 Distribution Plate 4 Well 6C
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persistent identity: iGEM 2018 Plate4 Well6C
display id: iGEM_2018_Plate4_Well6C
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member: TAL

iGEM 2018 Distribution Plate 4 Well 12C
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persistent identity: iGEM 2018 Plate4 Well12C
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version: 1
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member: BBa_K1150039

iGEM 2018 Distribution Plate 4 Well 19L
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persistent identity: iGEM 2018 Plate4 Well19L
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member: BBa_J23115

iGEM 2018 Distribution Plate 4 Well 13O
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member: BBa_I761014

iGEM 2018 Distribution Plate 4 Well 15M
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member: BBa_K747094

iGEM 2018 Distribution Plate 4 Well 6G
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member: BBa_K1104204

iGEM 2018 Distribution Plate 4 Well 3D
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member: cI HK022

iGEM 2018 Distribution Plate 4 Well 5B
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member: ecfp

iGEM 2018 Distribution Plate 4 Well 16C
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persistent identity: iGEM 2018 Plate4 Well16C
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version: 1
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member: BBa_J61100

iGEM 2018 Distribution Plate 4 Well 9D
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member: BBa_P0151

iGEM 2018 Distribution Plate 4 Well 7I
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member: BBa_I759017

iGEM 2018 Distribution Plate 4 Well 5I
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iGEM 2018 Distribution Plate 4 Well 13B
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member: BBa_I0466

iGEM 2018 Distribution Plate 4 Well 5C
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member: BBa_I746908

iGEM 2018 Distribution Plate 4 Well 7P
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iGEM 2018 Distribution Plate 4 Well 15I
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member: BBa_K115017

iGEM 2018 Distribution Plate 4 Well 5M
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member: BBa_I746101

iGEM 2018 Distribution Plate 4 Well 9C
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member: BBa_I759003

iGEM 2018 Distribution Plate 4 Well 11J
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member: BBa_I13602

iGEM 2018 Distribution Plate 4 Well 11H
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member: BBa_F2622

iGEM 2018 Distribution Plate 4 Well 12O
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member: BBa_K1061001

iGEM 2018 Distribution Plate 4 Well 5G
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member: BBa_K137019

iGEM 2018 Distribution Plate 4 Well 13G
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version: 1
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member: BBa_S03749

iGEM 2018 Distribution Plate 4
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member: iGEM 2018 Distribution Plate 4 Well 11H
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member: iGEM 2018 Distribution Plate 4 Well 12D
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member: iGEM 2018 Distribution Plate 4 Well 7C
member: iGEM 2018 Distribution Plate 4 Well 9H
member: iGEM 2018 Distribution Plate 4 Well 6H
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member: iGEM 2018 Distribution Plate 4 Well 4D
member: iGEM 2018 Distribution Plate 4 Well 1D
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member: iGEM 2018 Distribution Plate 4 Well 17H
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member: iGEM 2018 Distribution Plate 4 Well 17F
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member: iGEM 2018 Distribution Plate 4 Well 4B
member: iGEM 2018 Distribution Plate 4 Well 11J
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member: iGEM 2018 Distribution Plate 4 Well 11F
member: iGEM 2018 Distribution Plate 4 Well 6I
member: iGEM 2018 Distribution Plate 4 Well 3I
member: iGEM 2018 Distribution Plate 4 Well 7B

iGEM 2018 Distribution Plate 4 Well 13E
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persistent identity: iGEM 2018 Plate4 Well13E
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version: 1
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member: BBa_I759005

iGEM 2018 Distribution Plate 4 Well 21P
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persistent identity: iGEM 2018 Plate4 Well21P
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member: BBa_I13507

iGEM 2018 Distribution Plate 4 Well 11M
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display id: iGEM_2018_Plate4_Well11M
version: 1
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member: BBa_I732920

iGEM 2018 Distribution Plate 4 Well 4G
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persistent identity: iGEM 2018 Plate4 Well4G
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version: 1
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member: BBa_B0030

iGEM 2018 Distribution Plate 4 Well 6E
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version: 1
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member: BBa_K1092001

iGEM 2018 Distribution Plate 4 Well 2C
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persistent identity: iGEM 2018 Plate4 Well2C
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version: 1
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member: SgsE | CFP

iGEM 2018 Distribution Plate 4 Well 12G
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version: 1
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member: BBa_K1150041

iGEM 2018 Distribution Plate 4 Well 5J
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version: 1
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member: p(tetR)

iGEM 2018 Distribution Plate 4 Well 5N
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version: 1
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member: cII p22

iGEM 2018 Distribution Plate 4 Well 9B
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persistent identity: iGEM 2018 Plate4 Well9B
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version: 1
owned by: myers
member: BBa_S0105

iGEM 2018 Distribution Plate 4 Well 6B
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persistent identity: iGEM 2018 Plate4 Well6B
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version: 1
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member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 16M
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persistent identity: iGEM 2018 Plate4 Well16M
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version: 1
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member: yfp

iGEM 2018 Distribution Plate 4 Well 13M
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persistent identity: iGEM 2018 Plate4 Well13M
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version: 1
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member: BBa_I712669

iGEM 2018 Distribution Plate 4 Well 15N
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persistent identity: iGEM 2018 Plate4 Well15N
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version: 1
owned by: myers
member: OmpR

iGEM 2018 Distribution Plate 4 Well 5A
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persistent identity: iGEM 2018 Plate4 Well5A
display id: iGEM_2018_Plate4_Well5A
version: 1
owned by: myers
member: BBa_K137018

iGEM 2018 Distribution Plate 4 Well 21L
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persistent identity: iGEM 2018 Plate4 Well21L
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version: 1
owned by: myers
member: ecfp

iGEM 2018 Distribution Plate 4 Well 4C
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persistent identity: iGEM 2018 Plate4 Well4C
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version: 1
owned by: myers
member: BBa_J23108

iGEM 2018 Distribution Plate 4 Well 7H
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persistent identity: iGEM 2018 Plate4 Well7H
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version: 1
owned by: myers
member: lux pR

iGEM 2018 Distribution Plate 4 Well 7E
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persistent identity: iGEM 2018 Plate4 Well7E
display id: iGEM_2018_Plate4_Well7E
version: 1
owned by: myers
member: BBa_I759014

iGEM 2018 Distribution Plate 4 Well 6K
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persistent identity: iGEM 2018 Plate4 Well6K
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version: 1
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member: fwYellow

iGEM 2018 Distribution Plate 4 Well 3O
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persistent identity: iGEM 2018 Plate4 Well3O
display id: iGEM_2018_Plate4_Well3O
version: 1
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member: BBa_I746391

iGEM 2018 Distribution Plate 4 Well 14A
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persistent identity: iGEM 2018 Plate4 Well14A
display id: iGEM_2018_Plate4_Well14A
version: 1
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member: BBa_K1061003

iGEM 2018 Distribution Plate 4 Well 1O
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version: 1
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member: BBa_K118004

iGEM 2018 Distribution Plate 4 Well 2A
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persistent identity: iGEM 2018 Plate4 Well2A
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version: 1
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member: SgsE | YFP

iGEM 2018 Distribution Plate 4 Well 9N
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persistent identity: iGEM 2018 Plate4 Well9N
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version: 1
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member: PenI

iGEM 2018 Distribution Plate 4 Well 13C
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version: 1
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member: BBa_I712088

iGEM 2018 Distribution Plate 4 Well 3A
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persistent identity: iGEM 2018 Plate4 Well3A
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version: 1
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member: BBa_K118005

iGEM 2018 Distribution Plate 4 Well 15D
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persistent identity: iGEM 2018 Plate4 Well15D
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version: 1
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member: aiiA

iGEM 2018 Distribution Plate 4 Well 7C
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persistent identity: iGEM 2018 Plate4 Well7C
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version: 1
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member: BBa_I751100

iGEM 2018 Distribution Plate 4 Well 17O
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persistent identity: iGEM 2018 Plate4 Well17O
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version: 1
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member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 17P
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version: 1
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member: BBa_J23106

iGEM 2018 Distribution Plate 4 Well 6D
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persistent identity: iGEM 2018 Plate4 Well6D
display id: iGEM_2018_Plate4_Well6D
version: 1
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member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 20C
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persistent identity: iGEM 2018 Plate4 Well20C
display id: iGEM_2018_Plate4_Well20C
version: 1
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member: BBa_I712019

iGEM 2018 Distribution Plate 4 Well 21J
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persistent identity: iGEM 2018 Plate4 Well21J
display id: iGEM_2018_Plate4_Well21J
version: 1
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member: BBa_I13504

iGEM 2018 Distribution Plate 4 Well 11N
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persistent identity: iGEM 2018 Plate4 Well11N
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version: 1
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member: GFP report

iGEM 2018 Distribution Plate 4 Well 4E
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persistent identity: iGEM 2018 Plate4 Well4E
display id: iGEM_2018_Plate4_Well4E
version: 1
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member: BBa_J23109

iGEM 2018 Distribution Plate 4 Well 21N
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persistent identity: iGEM 2018 Plate4 Well21N
display id: iGEM_2018_Plate4_Well21N
version: 1
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member: ecfp

iGEM 2018 Distribution Plate 4 Well 19B
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persistent identity: iGEM 2018 Plate4 Well19B
display id: iGEM_2018_Plate4_Well19B
version: 1
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member: BBa_J23107

iGEM 2018 Distribution Plate 4 Well 12E
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persistent identity: iGEM 2018 Plate4 Well12E
display id: iGEM_2018_Plate4_Well12E
version: 1
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member: BBa_K1150040

iGEM 2018 Distribution Plate 4 Well 18C
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persistent identity: iGEM 2018 Plate4 Well18C
display id: iGEM_2018_Plate4_Well18C
version: 1
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member: GFP report

iGEM 2018 Distribution Plate 4 Well 21B
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persistent identity: iGEM 2018 Plate4 Well21B
display id: iGEM_2018_Plate4_Well21B
version: 1
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member: BBa_J23118

iGEM 2018 Distribution Plate 4 Well 16I
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persistent identity: iGEM 2018 Plate4 Well16I
display id: iGEM_2018_Plate4_Well16I
version: 1
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member: RFP2

iGEM 2018 Distribution Plate 4 Well 14O
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persistent identity: iGEM 2018 Plate4 Well14O
display id: iGEM_2018_Plate4_Well14O
version: 1
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member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 13I
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persistent identity: iGEM 2018 Plate4 Well13I
display id: iGEM_2018_Plate4_Well13I
version: 1
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member: BBa_I746902

iGEM 2018 Distribution Plate 4 Well 17N
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version: 1
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member: BBa_J23105

iGEM 2018 Distribution Plate 4 Well 11G
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display id: iGEM_2018_Plate4_Well11G
version: 1
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member: BBa_J52040

iGEM 2018 Distribution Plate 4 Well 3B
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persistent identity: iGEM 2018 Plate4 Well3B
display id: iGEM_2018_Plate4_Well3B
version: 1
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member: tetR

iGEM 2018 Distribution Plate 4 Well 23H
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persistent identity: iGEM 2018 Plate4 Well23H
display id: iGEM_2018_Plate4_Well23H
version: 1
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member: BBa_I13001

iGEM 2018 Distribution Plate 4 Well 11P
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persistent identity: iGEM 2018 Plate4 Well11P
display id: iGEM_2018_Plate4_Well11P
version: 1
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member: GFP genera

iGEM 2018 Distribution Plate 4 Well 10M
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version: 1
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member: BBa_K1150036

iGEM 2018 Distribution Plate 4 Well 8M
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version: 1
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member: BBa_K1150010

iGEM 2018 Distribution Plate 4 Well 8K
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persistent identity: iGEM 2018 Plate4 Well8K
display id: iGEM_2018_Plate4_Well8K
version: 1
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member: BBa_K1150007

iGEM 2018 Distribution Plate 4 Well 11L
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display id: iGEM_2018_Plate4_Well11L
version: 1
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member: BBa_I13401

iGEM 2018 Distribution Plate 4 Well 4A
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persistent identity: iGEM 2018 Plate4 Well4A
display id: iGEM_2018_Plate4_Well4A
version: 1
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member: BBa_J23111

iGEM 2018 Distribution Plate 4 Well 23J
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version: 1
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member: BBa_I13002

iGEM 2018 Distribution Plate 4 Well 13K
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version: 1
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member: PchA/PchB

iGEM 2018 Distribution Plate 4 Well 11I
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member: BBa_J45219

iGEM 2018 Distribution Plate 4 Well 13A
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version: 1
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member: BBa_I712058

iGEM 2018 Distribution Plate 4 Well 8I
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persistent identity: iGEM 2018 Plate4 Well8I
display id: iGEM_2018_Plate4_Well8I
version: 1
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member: BBa_K1150006

iGEM 2018 Distribution Plate 4 Well 15K
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persistent identity: iGEM 2018 Plate4 Well15K
display id: iGEM_2018_Plate4_Well15K
version: 1
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member: BBa_K115020

iGEM 2018 Distribution Plate 4 Well 10K
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persistent identity: iGEM 2018 Plate4 Well10K
display id: iGEM_2018_Plate4_Well10K
version: 1
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member: BBa_K1150035

iGEM 2018 Distribution Plate 4 Well 6F
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persistent identity: iGEM 2018 Plate4 Well6F
display id: iGEM_2018_Plate4_Well6F
version: 1
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member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 4B
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persistent identity: iGEM 2018 Plate4 Well4B
display id: iGEM_2018_Plate4_Well4B
version: 1
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member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 13H
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persistent identity: iGEM 2018 Plate4 Well13H
display id: iGEM_2018_Plate4_Well13H
version: 1
owned by: myers
member: BBa_E0044

iGEM 2018 Distribution Plate 4 Well 1D
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persistent identity: iGEM 2018 Plate4 Well1D
display id: iGEM_2018_Plate4_Well1D
version: 1
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member: BBa_B0024

iGEM 2018 Distribution Plate 4 Well 1K
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persistent identity: iGEM 2018 Plate4 Well1K
display id: iGEM_2018_Plate4_Well1K
version: 1
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member: BBa_K145279

iGEM 2018 Distribution Plate 4 Well 1C
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persistent identity: iGEM 2018 Plate4 Well1C
display id: iGEM_2018_Plate4_Well1C
version: 1
owned by: myers
member: T7pol

iGEM 2018 Distribution Plate 4 Well 17L
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persistent identity: iGEM 2018 Plate4 Well17L
display id: iGEM_2018_Plate4_Well17L
version: 1
owned by: myers
member: BBa_J23104

iGEM 2018 Distribution Plate 4 Well 2E
type: Collection
persistent identity: iGEM 2018 Plate4 Well2E
display id: iGEM_2018_Plate4_Well2E
version: 1
owned by: myers
member: SbpA | RFP

iGEM 2018 Distribution Plate 4 Well 17J
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persistent identity: iGEM 2018 Plate4 Well17J
display id: iGEM_2018_Plate4_Well17J
version: 1
owned by: myers
member: BBa_J23103

iGEM 2018 Distribution Plate 4 Well 2K
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persistent identity: iGEM 2018 Plate4 Well2K
display id: iGEM_2018_Plate4_Well2K
version: 1
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member: cjBlue

iGEM 2018 Distribution Plate 4 Well 23L
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persistent identity: iGEM 2018 Plate4 Well23L
display id: iGEM_2018_Plate4_Well23L
version: 1
owned by: myers
member: BBa_I13003

iGEM 2018 Distribution Plate 4 Well 7D
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persistent identity: iGEM 2018 Plate4 Well7D
display id: iGEM_2018_Plate4_Well7D
version: 1
owned by: myers
member: cI+luxR

iGEM 2018 Distribution Plate 4 Well 2H
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persistent identity: iGEM 2018 Plate4 Well2H
display id: iGEM_2018_Plate4_Well2H
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 1M
type: Collection
persistent identity: iGEM 2018 Plate4 Well1M
display id: iGEM_2018_Plate4_Well1M
version: 1
owned by: myers
member: dxs

iGEM 2018 Distribution Plate 4 Well 1H
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persistent identity: iGEM 2018 Plate4 Well1H
display id: iGEM_2018_Plate4_Well1H
version: 1
owned by: myers
member: BBa_B0031

iGEM 2018 Distribution Plate 4 Well 23P
type: Collection
persistent identity: iGEM 2018 Plate4 Well23P
display id: iGEM_2018_Plate4_Well23P
version: 1
owned by: myers
member: R6K

iGEM 2018 Distribution Plate 4 Well 12I
type: Collection
persistent identity: iGEM 2018 Plate4 Well12I
display id: iGEM_2018_Plate4_Well12I
version: 1
owned by: myers
member: BBa_K1150042

iGEM 2018 Distribution Plate 4 Well 8O
type: Collection
persistent identity: iGEM 2018 Plate4 Well8O
display id: iGEM_2018_Plate4_Well8O
version: 1
owned by: myers
member: BBa_K1150011

iGEM 2018 Distribution Plate 4 Well 8E
type: Collection
persistent identity: iGEM 2018 Plate4 Well8E
display id: iGEM_2018_Plate4_Well8E
version: 1
owned by: myers
member: BBa_K1150004

iGEM 2018 Distribution Plate 4 Well 11K
type: Collection
persistent identity: iGEM 2018 Plate4 Well11K
display id: iGEM_2018_Plate4_Well11K
version: 1
owned by: myers
member: BBa_J45299

iGEM 2018 Distribution Plate 4 Well 1A
type: Collection
persistent identity: iGEM 2018 Plate4 Well1A
display id: iGEM_2018_Plate4_Well1A
version: 1
owned by: myers
member: BBa_I746107

iGEM 2018 Distribution Plate 4 Well 11D
type: Collection
persistent identity: iGEM 2018 Plate4 Well11D
display id: iGEM_2018_Plate4_Well11D
version: 1
owned by: myers
member: RhlR+C4

iGEM 2018 Distribution Plate 4 Well 7J
type: Collection
persistent identity: iGEM 2018 Plate4 Well7J
display id: iGEM_2018_Plate4_Well7J
version: 1
owned by: myers
member: BBa_E0430

iGEM 2018 Distribution Plate 4 Well 8D
type: Collection
persistent identity: iGEM 2018 Plate4 Well8D
display id: iGEM_2018_Plate4_Well8D
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 4 Well 18A
type: Collection
persistent identity: iGEM 2018 Plate4 Well18A
display id: iGEM_2018_Plate4_Well18A
version: 1
owned by: myers
member: BBa_I20260

iGEM 2018 Distribution Plate 4 Well 15L
type: Collection
persistent identity: iGEM 2018 Plate4 Well15L
display id: iGEM_2018_Plate4_Well15L
version: 1
owned by: myers
member: OmpR

iGEM 2018 Distribution Plate 4 Well 13L
type: Collection
persistent identity: iGEM 2018 Plate4 Well13L
display id: iGEM_2018_Plate4_Well13L
version: 1
owned by: myers
member: GFP

iGEM 2018 Distribution Plate 4 Well 6M
type: Collection
persistent identity: iGEM 2018 Plate4 Well6M
display id: iGEM_2018_Plate4_Well6M
version: 1
owned by: myers
member: BBa_K1033916

iGEM 2018 Distribution Plate 4 Well 16G
type: Collection
persistent identity: iGEM 2018 Plate4 Well16G
display id: iGEM_2018_Plate4_Well16G
version: 1
owned by: myers
member: BBa_B0010

iGEM 2018 Distribution Plate 4 Well 5L
type: Collection
persistent identity: iGEM 2018 Plate4 Well5L
display id: iGEM_2018_Plate4_Well5L
version: 1
owned by: myers
member: cI lam

iGEM 2018 Distribution Plate 4 Well 17H
type: Collection
persistent identity: iGEM 2018 Plate4 Well17H
display id: iGEM_2018_Plate4_Well17H
version: 1
owned by: myers
member: BBa_J23102

iGEM 2018 Distribution Plate 4 Well 5H
type: Collection
persistent identity: iGEM 2018 Plate4 Well5H
display id: iGEM_2018_Plate4_Well5H
version: 1
owned by: myers
member: lacI+pL

iGEM 2018 Distribution Plate 4 Well 7F
type: Collection
persistent identity: iGEM 2018 Plate4 Well7F
display id: iGEM_2018_Plate4_Well7F
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owned by: myers
member: cI lam

iGEM 2018 Distribution Plate 4 Well 15J
type: Collection
persistent identity: iGEM 2018 Plate4 Well15J
display id: iGEM_2018_Plate4_Well15J
version: 1
owned by: myers
member: BBa_I13601

iGEM 2018 Distribution Plate 4 Well 3I
type: Collection
persistent identity: iGEM 2018 Plate4 Well3I
display id: iGEM_2018_Plate4_Well3I
version: 1
owned by: myers
member: rbs+LIMS1

iGEM 2018 Distribution Plate 4 Well 21H
type: Collection
persistent identity: iGEM 2018 Plate4 Well21H
display id: iGEM_2018_Plate4_Well21H
version: 1
owned by: myers
member: mRFP1

iGEM 2018 Distribution Plate 4 Well 10O
type: Collection
persistent identity: iGEM 2018 Plate4 Well10O
display id: iGEM_2018_Plate4_Well10O
version: 1
owned by: myers
member: BBa_K1150037

iGEM 2018 Distribution Plate 4 Well 12K
type: Collection
persistent identity: iGEM 2018 Plate4 Well12K
display id: iGEM_2018_Plate4_Well12K
version: 1
owned by: myers
member: BBa_K1150043

iGEM 2018 Distribution Plate 4 Well 5F
type: Collection
persistent identity: iGEM 2018 Plate4 Well5F
display id: iGEM_2018_Plate4_Well5F
version: 1
owned by: myers
member: YFP

iGEM 2018 Distribution Plate 4 Well 18G
type: Collection
persistent identity: iGEM 2018 Plate4 Well18G
display id: iGEM_2018_Plate4_Well18G
version: 1
owned by: myers
member: GFP report

iGEM 2018 Distribution Plate 4 Well 7L
type: Collection
persistent identity: iGEM 2018 Plate4 Well7L
display id: iGEM_2018_Plate4_Well7L
version: 1
owned by: myers
member: BBa_E0420

iGEM 2018 Distribution Plate 4 Well 11A
type: Collection
persistent identity: iGEM 2018 Plate4 Well11A
display id: iGEM_2018_Plate4_Well11A
version: 1
owned by: myers
member: BBa_J52014

iGEM 2018 Distribution Plate 4 Well 8G
type: Collection
persistent identity: iGEM 2018 Plate4 Well8G
display id: iGEM_2018_Plate4_Well8G
version: 1
owned by: myers
member: BBa_K1150005

BBa_I13002
type: ComponentDefinition
persistent identity: BBa I13002
display id: BBa_I13002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS B0031 w/ YFP (-LVA) TT
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0031
component: BBa_B0010
component: BBa_E0030
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13002_sequence/1

BBa_B0012
type: Component
persistent identity: component943469
display id: component943469
version: 1
top level: BBa_I13002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0031
type: Component
persistent identity: component943452
display id: component943452
version: 1
top level: BBa_I13002
access: public
definition: BBa_B0031

BBa_B0010
type: Component
persistent identity: component943459
display id: component943459
version: 1
top level: BBa_I13002
access: public
definition: BBa_B0010

BBa_E0030
type: Component
persistent identity: component943454
display id: component943454
version: 1
top level: BBa_I13002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943454
display id: annotation943454
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943454/range943454/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13002/annotation943454/range943454/1
type: Range
persistent identity: range943454
display id: range943454
version: 1
direction: direction/forward
top level: BBa_I13002
start: 21
end: 743
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943469
display id: annotation943469
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943469/range943469/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13002/annotation943469/range943469/1
type: Range
persistent identity: range943469
display id: range943469
version: 1
direction: direction/forward
top level: BBa_I13002
start: 840
end: 880
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation943452
display id: annotation943452
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943452/range943452/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I13002/annotation943452/range943452/1
type: Range
persistent identity: range943452
display id: range943452
version: 1
direction: direction/forward
top level: BBa_I13002
start: 1
end: 14
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943459
display id: annotation943459
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943459/range943459/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13002/annotation943459/range943459/1
type: Range
persistent identity: range943459
display id: range943459
version: 1
direction: direction/forward
top level: BBa_I13002
start: 752
end: 831
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR Protein Generator
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_C0062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
access: public
definition: BBa_B0010

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
access: public
definition: luxr

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
direction: direction/forward
top level: LuxR
start: 808
end: 887
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
direction: direction/forward
top level: LuxR
start: 19
end: 774
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
direction: direction/forward
top level: LuxR
start: 896
end: 936
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
direction: direction/forward
top level: LuxR
start: 1
end: 12
orientation: inline

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator
creator: Jennifer Braff
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0030.E0040.B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0030
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
access: public
definition: BBa_B0010

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
access: public
definition: BBa_B0030

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
access: public
definition: GFP

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
direction: direction/forward
top level: GFP genera
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
direction: direction/forward
top level: GFP genera
start: 22
end: 741
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
direction: direction/forward
top level: GFP genera
start: 838
end: 878
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
direction: direction/forward
top level: GFP genera
start: 750
end: 829
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.1 (strong) -- modified from R. Weiss
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
direction: direction/forward
top level: BBa_B0030
start: 8
end: 12
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J61000
display id: BBa_J61000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: chloramphenicol resistance cassette
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: Chloramphenicol resistance gene including its native promoter and ribosome binding site. Confers resistance to 25 ug/mL chloramphenicol. {pSB1A2-Bca1016}
mutable notes: N/A
mutable provenance: PCR ca1016F/R on pKD3 (934 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Products are Bca1015 and Bca1016 ---- ca1016F Forward EcoRI to biobrick CAT of pKD3 gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg ca1016R Reverse SpeI to biobrick CAT of pKD3 ccgctactagtggcgcgcctacctgtgacgg
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_J61000_sequence/1

CmR
type: SequenceAnnotation
persistent identity: annotation1892270
display id: annotation1892270
version: 1
top level: CmR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1

https://synbiohub.org/public/igem/BBa_J61000/annotation1892270/range1892270/1
type: Range
persistent identity: range1892270
display id: range1892270
version: 1
direction: direction/reverse
top level: CmR
start: 30
end: 687
orientation: inline

BBa_J61100
type: ComponentDefinition
persistent identity: BBa J61100
display id: BBa_J61100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: {{JCA_Arkin_RBSFamily}}
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61100_sequence/1

yfp
type: ComponentDefinition
persistent identity: BBa K165005
display id: BBa_K165005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Venus YFP, yeast optimized for fusion
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the venus YFP, yeast optimized in the Silver Lab BioFusion standard for fusion of protein domains.
mutable notes: -
mutable provenance: From Caroline Ajo-Franklin
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K165005_sequence/1

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR repressor/activator, (no LVA?)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: A
sequence annotation: luxR
sequence annotation: T
sequence annotation: BBa_C0062
sequence annotation: prefix
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
direction: direction/forward
top level: luxr
start: 757
end: 781
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
direction: direction/forward
top level: luxr
start: 492
end: 492
orientation: inline

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 750
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
direction: direction/forward
top level: luxr
start: 174
end: 174
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 756
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
direction: direction/forward
top level: luxr
start: 1
end: 2
orientation: inline

BBa_I759020
type: ComponentDefinition
persistent identity: BBa I759020
display id: BBa_I759020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cis8-repressed, tet-regulated YFP
creator: Kat Pak
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _115_
m user id: 0
owner id: 1834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has been shown to repress the expression of YFP via a cis-repressive element called cr8 which sequesters the ribosome binding site of YFP.
mutable provenance: The cr element is a synthetic oligo which has been inserted into part I759009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I759046
component: BBa_S03724
sequence annotation: BBa_I759046
sequence annotation: BBa_S03724
sequence: https://synbiohub.org/public/igem/BBa_I759020_sequence/1

BBa_I759046
type: Component
persistent identity: component2271257
display id: component2271257
version: 1
top level: BBa_I759020
access: public
definition: https://synbiohub.org/public/igem/BBa_I759046/1

BBa_S03724
type: Component
persistent identity: component2271280
display id: component2271280
version: 1
top level: BBa_I759020
access: public
definition: https://synbiohub.org/public/igem/BBa_S03724/1

BBa_I759046
type: SequenceAnnotation
persistent identity: annotation2271257
display id: annotation2271257
version: 1
top level: BBa_I759020
location: https://synbiohub.org/public/igem/BBa_I759020/annotation2271257/range2271257/1
component: BBa_I759046

https://synbiohub.org/public/igem/BBa_I759020/annotation2271257/range2271257/1
type: Range
persistent identity: range2271257
display id: range2271257
version: 1
direction: direction/reverse
top level: BBa_I759020
start: 1
end: 833
orientation: inline

BBa_S03724
type: SequenceAnnotation
persistent identity: annotation2271280
display id: annotation2271280
version: 1
top level: BBa_I759020
location: https://synbiohub.org/public/igem/BBa_I759020/annotation2271280/range2271280/1
component: BBa_S03724

https://synbiohub.org/public/igem/BBa_I759020/annotation2271280/range2271280/1
type: Range
persistent identity: range2271280
display id: range2271280
version: 1
direction: direction/reverse
top level: BBa_I759020
start: 842
end: 1861
orientation: inline

SgsE | YFP
type: ComponentDefinition
persistent identity: BBa K525305
display id: BBa_K525305
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and mCitrine
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T01:30:31Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Fusion Protein of S-Layer SgsE and mCitrine
mutable notes: Fusion Protein of BBa_K525303 and BBa_J18931
mutable provenance: Fusion Protein of S-Layer SgsE and mCitrine
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: ATG
sequence annotation: TAA
sequence annotation: K525301
sequence annotation: RBS
sequence annotation: T7 promoter
sequence annotation: SgsE domain
sequence annotation: NgoMIV
sequence annotation: AgeI
sequence annotation: B0034
sequence annotation: J18931
sequence annotation: mCitrine (YFP)
sequence annotation: Freiburg scar
sequence annotation: lac operator
sequence: https://synbiohub.org/public/igem/BBa_K525305_sequence/1

ATG
type: SequenceAnnotation
persistent identity: annotation2127333
display id: annotation2127333
version: 1
top level: SgsE | YFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127333/range2127333/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127333/range2127333/1
type: Range
persistent identity: range2127333
display id: range2127333
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 73
end: 75
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2127337
display id: annotation2127337
version: 1
top level: SgsE | YFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127337/range2127337/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127337/range2127337/1
type: Range
persistent identity: range2127337
display id: range2127337
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 3127
end: 3129
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2127339
display id: annotation2127339
version: 1
top level: SgsE | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127339/range2127339/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127339/range2127339/1
type: Range
persistent identity: range2127339
display id: range2127339
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 73
end: 2400
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2127330
display id: annotation2127330
version: 1
top level: SgsE | YFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127330/range2127330/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127330/range2127330/1
type: Range
persistent identity: range2127330
display id: range2127330
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 56
end: 59
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2127327
display id: annotation2127327
version: 1
top level: SgsE | YFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127327/range2127327/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127327/range2127327/1
type: Range
persistent identity: range2127327
display id: range2127327
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 1
end: 20
orientation: inline

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2127334
display id: annotation2127334
version: 1
top level: SgsE | YFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127334/range2127334/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127334/range2127334/1
type: Range
persistent identity: range2127334
display id: range2127334
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 73
end: 2400
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2127332
display id: annotation2127332
version: 1
top level: SgsE | YFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127332/range2127332/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127332/range2127332/1
type: Range
persistent identity: range2127332
display id: range2127332
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 76
end: 81
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2127338
display id: annotation2127338
version: 1
top level: SgsE | YFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127338/range2127338/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127338/range2127338/1
type: Range
persistent identity: range2127338
display id: range2127338
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 3121
end: 3126
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2127331
display id: annotation2127331
version: 1
top level: SgsE | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127331/range2127331/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127331/range2127331/1
type: Range
persistent identity: range2127331
display id: range2127331
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 53
end: 64
orientation: inline

J18931
type: SequenceAnnotation
persistent identity: annotation2127341
display id: annotation2127341
version: 1
top level: SgsE | YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127341/range2127341/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127341/range2127341/1
type: Range
persistent identity: range2127341
display id: range2127341
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 2407
end: 3129
orientation: inline

mCitrine (YFP)
type: SequenceAnnotation
persistent identity: annotation2127336
display id: annotation2127336
version: 1
top level: SgsE | YFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127336/range2127336/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127336/range2127336/1
type: Range
persistent identity: range2127336
display id: range2127336
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 2407
end: 3129
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2127335
display id: annotation2127335
version: 1
top level: SgsE | YFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127335/range2127335/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127335/range2127335/1
type: Range
persistent identity: range2127335
display id: range2127335
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 2401
end: 2406
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2127328
display id: annotation2127328
version: 1
top level: SgsE | YFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525305/annotation2127328/range2127328/1

https://synbiohub.org/public/igem/BBa_K525305/annotation2127328/range2127328/1
type: Range
persistent identity: range2127328
display id: range2127328
version: 1
direction: direction/forward
top level: SgsE | YFP
start: 20
end: 44
orientation: inline

BBa_I13003
type: ComponentDefinition
persistent identity: BBa I13003
display id: BBa_I13003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: medium RBS, YFP (-LVA), double terminator
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E0030
component: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0032
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13003_sequence/1

BBa_B0012
type: Component
persistent identity: component943495
display id: component943495
version: 1
top level: BBa_I13003
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component943485
display id: component943485
version: 1
top level: BBa_I13003
access: public
definition: BBa_B0010

BBa_E0030
type: Component
persistent identity: component943480
display id: component943480
version: 1
top level: BBa_I13003
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0032
type: Component
persistent identity: component943477
display id: component943477
version: 1
top level: BBa_I13003
access: public
definition: BBa_B0032

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943485
display id: annotation943485
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943485/range943485/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13003/annotation943485/range943485/1
type: Range
persistent identity: range943485
display id: range943485
version: 1
direction: direction/forward
top level: BBa_I13003
start: 751
end: 830
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation943477
display id: annotation943477
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943477/range943477/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13003/annotation943477/range943477/1
type: Range
persistent identity: range943477
display id: range943477
version: 1
direction: direction/forward
top level: BBa_I13003
start: 1
end: 13
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943480
display id: annotation943480
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943480/range943480/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13003/annotation943480/range943480/1
type: Range
persistent identity: range943480
display id: range943480
version: 1
direction: direction/forward
top level: BBa_I13003
start: 20
end: 742
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943495
display id: annotation943495
version: 1
top level: BBa_I13003
location: https://synbiohub.org/public/igem/BBa_I13003/annotation943495/range943495/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13003/annotation943495/range943495/1
type: Range
persistent identity: range943495
display id: range943495
version: 1
direction: direction/forward
top level: BBa_I13003
start: 839
end: 879
orientation: inline

BBa_I13601
type: ComponentDefinition
persistent identity: BBa I13601
display id: BBa_I13601
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac operator with CFP reporter (without LVA tag) [R/Lc-]
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0011
component: BBa_E0420
sequence annotation: BBa_R0011
sequence annotation: BBa_E0420
sequence: https://synbiohub.org/public/igem/BBa_I13601_sequence/1

BBa_R0011
type: Component
persistent identity: component2221463
display id: component2221463
version: 1
top level: BBa_I13601
access: public
definition: lacI+pL

BBa_E0420
type: Component
persistent identity: component2221477
display id: component2221477
version: 1
top level: BBa_I13601
access: public
definition: BBa_E0420

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2221463
display id: annotation2221463
version: 1
top level: BBa_I13601
location: https://synbiohub.org/public/igem/BBa_I13601/annotation2221463/range2221463/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13601/annotation2221463/range2221463/1
type: Range
persistent identity: range2221463
display id: range2221463
version: 1
direction: direction/forward
top level: BBa_I13601
start: 1
end: 54
orientation: inline

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2221477
display id: annotation2221477
version: 1
top level: BBa_I13601
location: https://synbiohub.org/public/igem/BBa_I13601/annotation2221477/range2221477/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_I13601/annotation2221477/range2221477/1
type: Range
persistent identity: range2221477
display id: range2221477
version: 1
direction: direction/reverse
top level: BBa_I13601
start: 64
end: 941
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.3 (medium) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: BBa_B0032
sequence annotation: RBS-3\Weak
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
direction: direction/forward
top level: BBa_B0032
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
direction: direction/forward
top level: BBa_B0032
start: 7
end: 10
orientation: inline

BBa_K1150005
type: ComponentDefinition
persistent identity: BBa K1150005
display id: BBa_K1150005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PIF6
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PIF6 ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PIF6
sequence: https://synbiohub.org/public/igem/BBa_K1150005_sequence/1

PIF6
type: SequenceAnnotation
persistent identity: annotation2347613
display id: annotation2347613
version: 1
top level: BBa_K1150005
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1150005/annotation2347613/range2347613/1

https://synbiohub.org/public/igem/BBa_K1150005/annotation2347613/range2347613/1
type: Range
persistent identity: range2347613
display id: range2347613
version: 1
direction: direction/forward
top level: BBa_K1150005
start: 1
end: 300
orientation: inline

BBa_K1150035
type: ComponentDefinition
persistent identity: BBa K1150035
display id: BBa_K1150035
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: uniCAS RNAimer to EMX1
creator: Ilona Blank
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ..
mutable notes: ..
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1150035_sequence/1

BBa_J23118
type: ComponentDefinition
persistent identity: BBa J23118
display id: BBa_J23118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23118_sequence/1

BBa_I746908
type: ComponentDefinition
persistent identity: BBa I746908
display id: BBa_I746908
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: superfolder GFP driven by pBAD promoter
creator: Stefan Milde
created: 2008-09-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is one of the constructs used to characterise superfolder GFP (see I746916 part description for source and other information about this GFP variant)
mutable notes: no special considerations
mutable provenance: This part was created by standard assembly from construction intermediates: I746910 was put together with I746916:B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0034
component: BBa_I0500
component: BBa_B0010
component: BBa_I746916
sequence annotation: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_I746916
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I746908_sequence/1

BBa_B0012
type: Component
persistent identity: component1977589
display id: component1977589
version: 1
top level: BBa_I746908
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1977582
display id: component1977582
version: 1
top level: BBa_I746908
access: public
definition: BBa_B0034

BBa_I0500
type: Component
persistent identity: component1977580
display id: component1977580
version: 1
top level: BBa_I746908
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
type: Component
persistent identity: component1977587
display id: component1977587
version: 1
top level: BBa_I746908
access: public
definition: BBa_B0010

BBa_I746916
type: Component
persistent identity: component1977586
display id: component1977586
version: 1
top level: BBa_I746908
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1977580
display id: annotation1977580
version: 1
top level: BBa_I746908
location: https://synbiohub.org/public/igem/BBa_I746908/annotation1977580/range1977580/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I746908/annotation1977580/range1977580/1
type: Range
persistent identity: range1977580
display id: range1977580
version: 1
direction: direction/reverse
top level: BBa_I746908
start: 1
end: 1210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1977589
display id: annotation1977589
version: 1
top level: BBa_I746908
location: https://synbiohub.org/public/igem/BBa_I746908/annotation1977589/range1977589/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746908/annotation1977589/range1977589/1
type: Range
persistent identity: range1977589
display id: range1977589
version: 1
direction: direction/forward
top level: BBa_I746908
start: 2053
end: 2093
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation1977586
display id: annotation1977586
version: 1
top level: BBa_I746908
location: https://synbiohub.org/public/igem/BBa_I746908/annotation1977586/range1977586/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_I746908/annotation1977586/range1977586/1
type: Range
persistent identity: range1977586
display id: range1977586
version: 1
direction: direction/reverse
top level: BBa_I746908
start: 1237
end: 1956
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1977587
display id: annotation1977587
version: 1
top level: BBa_I746908
location: https://synbiohub.org/public/igem/BBa_I746908/annotation1977587/range1977587/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746908/annotation1977587/range1977587/1
type: Range
persistent identity: range1977587
display id: range1977587
version: 1
direction: direction/forward
top level: BBa_I746908
start: 1965
end: 2044
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1977582
display id: annotation1977582
version: 1
top level: BBa_I746908
location: https://synbiohub.org/public/igem/BBa_I746908/annotation1977582/range1977582/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746908/annotation1977582/range1977582/1
type: Range
persistent identity: range1977582
display id: range1977582
version: 1
direction: direction/reverse
top level: BBa_I746908
start: 1219
end: 1230
orientation: inline

BBa_I712088
type: ComponentDefinition
persistent identity: BBa I712088
display id: BBa_I712088
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV - CD4(shortened) - Cub - GFP
creator: Marko Bitenc
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1835
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CD4 - Cub fused with GFP signal under cmv promoter.
mutable notes: /
mutable provenance: Ligation of two biobricks(BBa_I712085, BBa_J52642).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I712011
component: BBa_I712082
component: BBa_J52642
sequence annotation: BBa_I712011
sequence annotation: BBa_I712082
sequence annotation: BBa_J52642
sequence: https://synbiohub.org/public/igem/BBa_I712088_sequence/1

BBa_I712011
type: Component
persistent identity: component1953294
display id: component1953294
version: 1
top level: BBa_I712088
access: public
definition: https://synbiohub.org/public/igem/BBa_I712011/1

BBa_I712082
type: Component
persistent identity: component1953293
display id: component1953293
version: 1
top level: BBa_I712088
access: public
definition: https://synbiohub.org/public/igem/BBa_I712082/1

BBa_J52642
type: Component
persistent identity: component1953298
display id: component1953298
version: 1
top level: BBa_I712088
access: public
definition: https://synbiohub.org/public/igem/BBa_J52642/1

BBa_I712011
type: SequenceAnnotation
persistent identity: annotation1953294
display id: annotation1953294
version: 1
top level: BBa_I712088
location: https://synbiohub.org/public/igem/BBa_I712088/annotation1953294/range1953294/1
component: BBa_I712011

https://synbiohub.org/public/igem/BBa_I712088/annotation1953294/range1953294/1
type: Range
persistent identity: range1953294
display id: range1953294
version: 1
direction: direction/reverse
top level: BBa_I712088
start: 1914
end: 2048
orientation: inline

BBa_I712082
type: SequenceAnnotation
persistent identity: annotation1953293
display id: annotation1953293
version: 1
top level: BBa_I712088
location: https://synbiohub.org/public/igem/BBa_I712088/annotation1953293/range1953293/1
component: BBa_I712082

https://synbiohub.org/public/igem/BBa_I712088/annotation1953293/range1953293/1
type: Range
persistent identity: range1953293
display id: range1953293
version: 1
direction: direction/reverse
top level: BBa_I712088
start: 1
end: 1907
orientation: inline

BBa_J52642
type: SequenceAnnotation
persistent identity: annotation1953298
display id: annotation1953298
version: 1
top level: BBa_I712088
location: https://synbiohub.org/public/igem/BBa_I712088/annotation1953298/range1953298/1
component: BBa_J52642

https://synbiohub.org/public/igem/BBa_I712088/annotation1953298/range1953298/1
type: Range
persistent identity: range1953298
display id: range1953298
version: 1
direction: direction/reverse
top level: BBa_I712088
start: 2055
end: 2774
orientation: inline

RFP2
type: ComponentDefinition
persistent identity: BBa I715023
display id: BBa_I715023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Carboxyl portion of RFP
creator: Andrew Martens
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:44:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
sample status: In stock
status: status/Available
bookmark: false
mutable description: The second portion of the RFP gene, after the HixC insertion point.
mutable notes: Used web tool.
mutable provenance: Sequence from part BBa_E1010.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: extra base (avoid frameshift)
sequence annotation: Continue coding sequence
sequence annotation: (two in tandem)
sequence: https://synbiohub.org/public/igem/BBa_I715023_sequence/1

extra base (avoid frameshift)
type: SequenceAnnotation
persistent identity: annotation1935382
display id: annotation1935382
version: 1
top level: RFP2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1

https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1
type: Range
persistent identity: range1935382
display id: range1935382
version: 1
direction: direction/forward
top level: RFP2
start: 1
end: 1
orientation: inline

Continue coding sequence
type: SequenceAnnotation
persistent identity: annotation1935383
display id: annotation1935383
version: 1
top level: RFP2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1

https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1
type: Range
persistent identity: range1935383
display id: range1935383
version: 1
direction: direction/forward
top level: RFP2
start: 2
end: 214
orientation: inline

(two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935384
display id: annotation1935384
version: 1
top level: RFP2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1

https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1
type: Range
persistent identity: range1935384
display id: range1935384
version: 1
direction: direction/forward
top level: RFP2
start: 215
end: 219
orientation: inline

BBa_K1150012
type: ComponentDefinition
persistent identity: BBa K1150012
display id: BBa_K1150012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BGH terminator
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ..
mutable notes: ..
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BGH
sequence: https://synbiohub.org/public/igem/BBa_K1150012_sequence/1

BGH
type: SequenceAnnotation
persistent identity: annotation2347917
display id: annotation2347917
version: 1
top level: BBa_K1150012
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1150012/annotation2347917/range2347917/1

https://synbiohub.org/public/igem/BBa_K1150012/annotation2347917/range2347917/1
type: Range
persistent identity: range2347917
display id: range2347917
version: 1
direction: direction/forward
top level: BBa_K1150012
start: 1
end: 222
orientation: inline

BBa_K145280
type: ComponentDefinition
persistent identity: BBa K145280
display id: BBa_K145280
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP-LVA under TetR control
creator: Nathalie Busschaert
created: 2008-09-06T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device consists of a TetR generator and a GFP with LVA tag under control of a TetR promoter. Normally the production of GFP-LVA is switched off. It can be switched on by the addition of tetracyclin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K145201
component: BBa_K145205
sequence annotation: BBa_K145205
sequence annotation: BBa_K145201
sequence: https://synbiohub.org/public/igem/BBa_K145280_sequence/1

BBa_K145201
type: Component
persistent identity: component2282043
display id: component2282043
version: 1
top level: BBa_K145280
access: public
definition: https://synbiohub.org/public/igem/BBa_K145201/1

BBa_K145205
type: Component
persistent identity: component2282064
display id: component2282064
version: 1
top level: BBa_K145280
access: public
definition: https://synbiohub.org/public/igem/BBa_K145205/1

BBa_K145205
type: SequenceAnnotation
persistent identity: annotation2282064
display id: annotation2282064
version: 1
top level: BBa_K145280
location: https://synbiohub.org/public/igem/BBa_K145280/annotation2282064/range2282064/1
component: BBa_K145205

https://synbiohub.org/public/igem/BBa_K145280/annotation2282064/range2282064/1
type: Range
persistent identity: range2282064
display id: range2282064
version: 1
direction: direction/reverse
top level: BBa_K145280
start: 892
end: 1868
orientation: inline

BBa_K145201
type: SequenceAnnotation
persistent identity: annotation2282043
display id: annotation2282043
version: 1
top level: BBa_K145280
location: https://synbiohub.org/public/igem/BBa_K145280/annotation2282043/range2282043/1
component: BBa_K145201

https://synbiohub.org/public/igem/BBa_K145280/annotation2282043/range2282043/1
type: Range
persistent identity: range2282043
display id: range2282043
version: 1
direction: direction/reverse
top level: BBa_K145280
start: 1
end: 883
orientation: inline

BBa_I751102
type: ComponentDefinition
persistent identity: BBa I751102
display id: BBa_I751102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J54140dP + hybrid promoter (lux-cI)
creator: Kenichiro Iwasaki
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A4HPcI
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BamHI
sequence annotation: MluI
sequence annotation: J54025
sequence annotation: B0012
sequence annotation: C0062
sequence annotation: SalI
sequence annotation: E0040
sequence annotation: BglII
sequence annotation: R0063
sequence annotation: B0010
sequence annotation: plux-cI hybrid promoter
sequence annotation: B0034
sequence annotation: J54033
sequence annotation: B0030
sequence: https://synbiohub.org/public/igem/BBa_I751102_sequence/1

BamHI
type: SequenceAnnotation
persistent identity: annotation1954840
display id: annotation1954840
version: 1
top level: BBa_I751102
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954840/range1954840/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954840/range1954840/1
type: Range
persistent identity: range1954840
display id: range1954840
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1116
end: 1121
orientation: inline

MluI
type: SequenceAnnotation
persistent identity: annotation1954863
display id: annotation1954863
version: 1
top level: BBa_I751102
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954863/range1954863/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954863/range1954863/1
type: Range
persistent identity: range1954863
display id: range1954863
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1214
end: 1219
orientation: inline

J54025
type: SequenceAnnotation
persistent identity: annotation1954864
display id: annotation1954864
version: 1
top level: BBa_I751102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954864/range1954864/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954864/range1954864/1
type: Range
persistent identity: range1954864
display id: range1954864
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1200
end: 1219
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation1954868
display id: annotation1954868
version: 1
top level: BBa_I751102
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954868/range1954868/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954868/range1954868/1
type: Range
persistent identity: range1954868
display id: range1954868
version: 1
direction: direction/forward
top level: BBa_I751102
start: 2065
end: 2105
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation1954837
display id: annotation1954837
version: 1
top level: BBa_I751102
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954837/range1954837/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954837/range1954837/1
type: Range
persistent identity: range1954837
display id: range1954837
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1055
end: 1095
orientation: inline

C0062
type: SequenceAnnotation
persistent identity: annotation1954835
display id: annotation1954835
version: 1
top level: BBa_I751102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954835/range1954835/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954835/range1954835/1
type: Range
persistent identity: range1954835
display id: range1954835
version: 1
direction: direction/forward
top level: BBa_I751102
start: 178
end: 958
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation1954839
display id: annotation1954839
version: 1
top level: BBa_I751102
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954839/range1954839/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954839/range1954839/1
type: Range
persistent identity: range1954839
display id: range1954839
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1104
end: 1109
orientation: inline

E0040
type: SequenceAnnotation
persistent identity: annotation1954866
display id: annotation1954866
version: 1
top level: BBa_I751102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954866/range1954866/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954866/range1954866/1
type: Range
persistent identity: range1954866
display id: range1954866
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1249
end: 1967
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1954852
display id: annotation1954852
version: 1
top level: BBa_I751102
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954852/range1954852/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954852/range1954852/1
type: Range
persistent identity: range1954852
display id: range1954852
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1200
end: 1205
orientation: inline

R0063
type: SequenceAnnotation
persistent identity: annotation1954833
display id: annotation1954833
version: 1
top level: BBa_I751102
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954833/range1954833/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954833/range1954833/1
type: Range
persistent identity: range1954833
display id: range1954833
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1
end: 151
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation1954867
display id: annotation1954867
version: 1
top level: BBa_I751102
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954867/range1954867/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954867/range1954867/1
type: Range
persistent identity: range1954867
display id: range1954867
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1977
end: 2055
orientation: inline

plux-cI hybrid promoter
type: SequenceAnnotation
persistent identity: annotation1954841
display id: annotation1954841
version: 1
top level: BBa_I751102
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954841/range1954841/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954841/range1954841/1
type: Range
persistent identity: range1954841
display id: range1954841
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1122
end: 1187
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation1954836
display id: annotation1954836
version: 1
top level: BBa_I751102
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954836/range1954836/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954836/range1954836/1
type: Range
persistent identity: range1954836
display id: range1954836
version: 1
direction: direction/forward
top level: BBa_I751102
start: 967
end: 1046
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1954834
display id: annotation1954834
version: 1
top level: BBa_I751102
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954834/range1954834/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954834/range1954834/1
type: Range
persistent identity: range1954834
display id: range1954834
version: 1
direction: direction/forward
top level: BBa_I751102
start: 160
end: 171
orientation: inline

J54033
type: SequenceAnnotation
persistent identity: annotation1954838
display id: annotation1954838
version: 1
top level: BBa_I751102
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954838/range1954838/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954838/range1954838/1
type: Range
persistent identity: range1954838
display id: range1954838
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1104
end: 1121
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation1954865
display id: annotation1954865
version: 1
top level: BBa_I751102
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I751102/annotation1954865/range1954865/1

https://synbiohub.org/public/igem/BBa_I751102/annotation1954865/range1954865/1
type: Range
persistent identity: range1954865
display id: range1954865
version: 1
direction: direction/forward
top level: BBa_I751102
start: 1228
end: 1242
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR repressible promoter
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: TetR 1
sequence annotation: BBa_R0040
sequence annotation: TetR 2
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
direction: direction/forward
top level: p(tetR)
start: 43
end: 48
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 19
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
direction: direction/forward
top level: p(tetR)
start: 1
end: 54
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
direction: direction/forward
top level: p(tetR)
start: 26
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
direction: direction/forward
top level: p(tetR)
start: 20
end: 25
orientation: inline

BBa_K125500
type: ComponentDefinition
persistent identity: BBa K125500
display id: BBa_K125500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fusion brick
creator: Grace Kwan
created: 2008-07-24T11:00:00Z
modified: 2016-01-25T02:34:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 4206
owner id: 3229
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The first two nucleotides of the GFP start codon were removed to create this fusion brick.
mutable provenance: The GFP fusion brick was derived from the GFP BioBrick, [http://partsregistry.org/Part:BBa_E0040 BBa_E0040].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP fusion brick
sequence annotation: deletion of TG
sequence: https://synbiohub.org/public/igem/BBa_K125500_sequence/1

GFP fusion brick
type: SequenceAnnotation
persistent identity: annotation1968260
display id: annotation1968260
version: 1
top level: BBa_K125500
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1

https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1
type: Range
persistent identity: range1968260
display id: range1968260
version: 1
direction: direction/forward
top level: BBa_K125500
start: 1
end: 718
orientation: inline

deletion of TG
type: SequenceAnnotation
persistent identity: annotation1969908
display id: annotation1969908
version: 1
top level: BBa_K125500
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1

https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1
type: Range
persistent identity: range1969908
display id: range1969908
version: 1
direction: direction/forward
top level: BBa_K125500
start: 1
end: 1
orientation: inline

BBa_J23112
type: ComponentDefinition
persistent identity: BBa J23112
display id: BBa_J23112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23112_sequence/1

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

BBa_J52038
type: ComponentDefinition
persistent identity: BBa J52038
display id: BBa_J52038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV + Rluc
creator: Monika Ciglic
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: CMV + Rluc is a composite part made of constitutive promotor CMV and Rluc, which acts as a reporter.
mutable notes: This compositive part is cloned in vector BBa_J52017 (we have made it by ligating eucaryotic terminator in BioBrick vector pSB1AK3).
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J52008
component: BBa_J52034
sequence annotation: BBa_J52008
sequence annotation: BBa_J52034
sequence: https://synbiohub.org/public/igem/BBa_J52038_sequence/1

BBa_J52008
type: Component
persistent identity: component1903154
display id: component1903154
version: 1
top level: BBa_J52038
access: public
definition: https://synbiohub.org/public/igem/BBa_J52008/1

BBa_J52034
type: Component
persistent identity: component1903151
display id: component1903151
version: 1
top level: BBa_J52038
access: public
definition: https://synbiohub.org/public/igem/BBa_J52034/1

BBa_J52008
type: SequenceAnnotation
persistent identity: annotation1903154
display id: annotation1903154
version: 1
top level: BBa_J52038
location: https://synbiohub.org/public/igem/BBa_J52038/annotation1903154/range1903154/1
component: BBa_J52008

https://synbiohub.org/public/igem/BBa_J52038/annotation1903154/range1903154/1
type: Range
persistent identity: range1903154
display id: range1903154
version: 1
direction: direction/reverse
top level: BBa_J52038
start: 661
end: 1596
orientation: inline

BBa_J52034
type: SequenceAnnotation
persistent identity: annotation1903151
display id: annotation1903151
version: 1
top level: BBa_J52038
location: https://synbiohub.org/public/igem/BBa_J52038/annotation1903151/range1903151/1
component: BBa_J52034

https://synbiohub.org/public/igem/BBa_J52038/annotation1903151/range1903151/1
type: Range
persistent identity: range1903151
display id: range1903151
version: 1
direction: direction/reverse
top level: BBa_J52038
start: 1
end: 654
orientation: inline

BBa_K115001
type: ComponentDefinition
persistent identity: BBa K115001
display id: BBa_K115001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA thermometer (ROSE)
creator: O.M.J.A. Stassen
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3007
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is designed as a temperature inducible RBS. Only designed so far.
mutable notes: The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
mutable provenance: This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein (prfA).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: Predicted Stem Loop
sequence annotation: Predicted Stem Loop extending to startcodon
sequence annotation: SD
sequence annotation: Biobrick-Scar-adaptation
sequence: https://synbiohub.org/public/igem/BBa_K115001_sequence/1

Predicted Stem Loop
type: SequenceAnnotation
persistent identity: annotation1966928
display id: annotation1966928
version: 1
top level: BBa_K115001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966928/range1966928/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966928/range1966928/1
type: Range
persistent identity: range1966928
display id: range1966928
version: 1
direction: direction/forward
top level: BBa_K115001
start: 39
end: 71
orientation: inline

Predicted Stem Loop extending to startcodon
type: SequenceAnnotation
persistent identity: annotation1966929
display id: annotation1966929
version: 1
top level: BBa_K115001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966929/range1966929/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966929/range1966929/1
type: Range
persistent identity: range1966929
display id: range1966929
version: 1
direction: direction/forward
top level: BBa_K115001
start: 72
end: 96
orientation: inline

Predicted Stem Loop
type: SequenceAnnotation
persistent identity: annotation1966926
display id: annotation1966926
version: 1
top level: BBa_K115001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966926/range1966926/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966926/range1966926/1
type: Range
persistent identity: range1966926
display id: range1966926
version: 1
direction: direction/forward
top level: BBa_K115001
start: 1
end: 15
orientation: inline

Predicted Stem Loop
type: SequenceAnnotation
persistent identity: annotation1966927
display id: annotation1966927
version: 1
top level: BBa_K115001
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966927/range1966927/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966927/range1966927/1
type: Range
persistent identity: range1966927
display id: range1966927
version: 1
direction: direction/forward
top level: BBa_K115001
start: 16
end: 36
orientation: inline

SD
type: SequenceAnnotation
persistent identity: annotation1966896
display id: annotation1966896
version: 1
top level: BBa_K115001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966896/range1966896/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966896/range1966896/1
type: Range
persistent identity: range1966896
display id: range1966896
version: 1
direction: direction/forward
top level: BBa_K115001
start: 90
end: 95
orientation: inline

Biobrick-Scar-adaptation
type: SequenceAnnotation
persistent identity: annotation1966898
display id: annotation1966898
version: 1
top level: BBa_K115001
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K115001/annotation1966898/range1966898/1

https://synbiohub.org/public/igem/BBa_K115001/annotation1966898/range1966898/1
type: Range
persistent identity: range1966898
display id: range1966898
version: 1
direction: direction/forward
top level: BBa_K115001
start: 74
end: 79
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_K1188002
type: ComponentDefinition
persistent identity: BBa K1188002
display id: BBa_K1188002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RTX - Calcium Binding Protein
creator: Jean-Francois Lalonde
created: 2013-08-22T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1501_
m user id: 0
owner id: 17738
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: RTX (repeats-in-toxin) is a polypeptide motif consisting of a repeating sequence of amino acids. It undergoes a conformation change in the presence of calcium which causes it to precipitate from solution. This is the shorter (8-mer) version of this part, and is in Freiburg format. This allows RTX to be fused to itself to create a longer RTX chain, or to other proteins which can then be precipitated from a solution. This is useful for the purpose of protein purification, or alternatively to remove an unwanted protein from a solution.
mutable notes: We ordered PCR primers to isolate RTX from its original construct. Due to the repetitive nature of this construct, we found that there were multiple binding sites for the forward primer which resulted in two products. This is the shorter PCR product, with only 8 repeats, whereas the original construct had 17.
mutable provenance: J Biol Chem. 2011 May 13;286(19):16997-7004 http://www.ncbi.nlm.nih.gov/pubmed/21454565
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K1188002_sequence/1

BBa_K1104204
type: ComponentDefinition
persistent identity: BBa K1104204
display id: BBa_K1104204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AhpCp2D1
creator: Ting-Yun Chiang
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1415_
m user id: 0
owner id: 16313
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: We improve the function of a BioBrick Part: AhpC promoter([http://parts.igem.org/Part:BBa_K362001 K362001])designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team] into four versions.On PartRegistry, the complex part(according to [http://ecocyc.org/ECOLI/new-image?object=EG11384 Ecocyc]) composition contains hybrid promoters, shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG. This part contains 3 promoters: AhpC1, DsbG, AhpC2, 2 TFBSs for OxyR, and coding sequence in the initiate part([http://parts.igem.org/Part:BBa_K362001 K362001])was deleted.
mutable notes: We delete the dsbG coding and following sequence next to dsbG reverse primer, making the functional sequence shorter, so that PCR can be more easy and efficient.
mutable provenance: ''E. coli MG1655'' genomic sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: ctgcag->ctacag
sequence annotation: OxyR binding site3
sequence annotation: (AhpCp1)-10
sequence annotation: (AhpCp2)-35
sequence annotation: g->a
sequence annotation: (AhpCp1)-35
sequence annotation: DsbGp
sequence annotation: OxyR binding site2
sequence annotation: OxyR binding site4
sequence annotation: OxyR binding site1
sequence annotation: (AhpCp2)-10
sequence: https://synbiohub.org/public/igem/BBa_K1104204_sequence/1

ctgcag->ctacag
type: SequenceAnnotation
persistent identity: annotation2365171
display id: annotation2365171
version: 1
top level: BBa_K1104204
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365171/range2365171/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365171/range2365171/1
type: Range
persistent identity: range2365171
display id: range2365171
version: 1
direction: direction/forward
top level: BBa_K1104204
start: 233
end: 238
orientation: inline

OxyR binding site3
type: SequenceAnnotation
persistent identity: annotation2365174
display id: annotation2365174
version: 1
top level: BBa_K1104204
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365174/range2365174/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365174/range2365174/1
type: Range
persistent identity: range2365174
display id: range2365174
version: 1
direction: direction/forward
top level: BBa_K1104204
start: 356
end: 372
orientation: inline

(AhpCp1)-10
type: SequenceAnnotation
persistent identity: annotation2365177
display id: annotation2365177
version: 1
top level: BBa_K1104204
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365177/range2365177/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365177/range2365177/1
type: Range
persistent identity: range2365177
display id: range2365177
version: 1
direction: direction/forward
top level: BBa_K1104204
start: 417
end: 422
orientation: inline

(AhpCp2)-35
type: SequenceAnnotation
persistent identity: annotation2365169
display id: annotation2365169
version: 1
top level: BBa_K1104204
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365169/range2365169/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365169/range2365169/1
type: Range
persistent identity: range2365169
display id: range2365169
version: 1
direction: direction/forward
top level: BBa_K1104204
start: 166
end: 171
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation2365172
display id: annotation2365172
version: 1
top level: BBa_K1104204
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365172/range2365172/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365172/range2365172/1
type: Range
persistent identity: range2365172
display id: range2365172
version: 1
direction: direction/forward
top level: BBa_K1104204
start: 235
end: 235
orientation: inline

(AhpCp1)-35
type: SequenceAnnotation
persistent identity: annotation2365176
display id: annotation2365176
version: 1
top level: BBa_K1104204
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365176/range2365176/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365176/range2365176/1
type: Range
persistent identity: range2365176
display id: range2365176
version: 1
direction: direction/forward
top level: BBa_K1104204
start: 393
end: 398
orientation: inline

DsbGp
type: SequenceAnnotation
persistent identity: annotation2365173
display id: annotation2365173
version: 1
top level: BBa_K1104204
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365173/range2365173/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365173/range2365173/1
type: Range
persistent identity: range2365173
display id: range2365173
version: 1
direction: direction/reverse
top level: BBa_K1104204
start: 285
end: 309
orientation: inline

OxyR binding site2
type: SequenceAnnotation
persistent identity: annotation2365168
display id: annotation2365168
version: 1
top level: BBa_K1104204
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365168/range2365168/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365168/range2365168/1
type: Range
persistent identity: range2365168
display id: range2365168
version: 1
direction: direction/forward
top level: BBa_K1104204
start: 141
end: 157
orientation: inline

OxyR binding site4
type: SequenceAnnotation
persistent identity: annotation2365175
display id: annotation2365175
version: 1
top level: BBa_K1104204
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365175/range2365175/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365175/range2365175/1
type: Range
persistent identity: range2365175
display id: range2365175
version: 1
direction: direction/forward
top level: BBa_K1104204
start: 378
end: 394
orientation: inline

OxyR binding site1
type: SequenceAnnotation
persistent identity: annotation2365167
display id: annotation2365167
version: 1
top level: BBa_K1104204
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365167/range2365167/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365167/range2365167/1
type: Range
persistent identity: range2365167
display id: range2365167
version: 1
direction: direction/forward
top level: BBa_K1104204
start: 118
end: 137
orientation: inline

(AhpCp2)-10
type: SequenceAnnotation
persistent identity: annotation2365170
display id: annotation2365170
version: 1
top level: BBa_K1104204
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1104204/annotation2365170/range2365170/1

https://synbiohub.org/public/igem/BBa_K1104204/annotation2365170/range2365170/1
type: Range
persistent identity: range2365170
display id: range2365170
version: 1
direction: direction/forward
top level: BBa_K1104204
start: 192
end: 197
orientation: inline

BBa_I746381
type: ComponentDefinition
persistent identity: BBa I746381
display id: BBa_I746381
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PSP3 pag inducing PO promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the PSP3 pag activator on the PO promoter. Note that the I746351 does contain a RBS.
mutable notes: I746314 was put upstream of I746321
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746314
component: BBa_I746321
sequence annotation: BBa_I746314
sequence annotation: BBa_I746321
sequence: https://synbiohub.org/public/igem/BBa_I746381_sequence/1

BBa_I746314
type: Component
persistent identity: component2291883
display id: component2291883
version: 1
top level: BBa_I746381
access: public
definition: https://synbiohub.org/public/igem/BBa_I746314/1

BBa_I746321
type: Component
persistent identity: component2291896
display id: component2291896
version: 1
top level: BBa_I746381
access: public
definition: https://synbiohub.org/public/igem/BBa_I746321/1

BBa_I746314
type: SequenceAnnotation
persistent identity: annotation2291883
display id: annotation2291883
version: 1
top level: BBa_I746381
location: https://synbiohub.org/public/igem/BBa_I746381/annotation2291883/range2291883/1
component: BBa_I746314

https://synbiohub.org/public/igem/BBa_I746381/annotation2291883/range2291883/1
type: Range
persistent identity: range2291883
display id: range2291883
version: 1
direction: direction/reverse
top level: BBa_I746381
start: 1
end: 2324
orientation: inline

BBa_I746321
type: SequenceAnnotation
persistent identity: annotation2291896
display id: annotation2291896
version: 1
top level: BBa_I746381
location: https://synbiohub.org/public/igem/BBa_I746381/annotation2291896/range2291896/1
component: BBa_I746321

https://synbiohub.org/public/igem/BBa_I746381/annotation2291896/range2291896/1
type: Range
persistent identity: range2291896
display id: range2291896
version: 1
direction: direction/reverse
top level: BBa_I746381
start: 2333
end: 3307
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Replace later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_I746101
type: ComponentDefinition
persistent identity: BBa I746101
display id: BBa_I746101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I746100 (AIP sensor infrastructure - agrC + agrA with RBSes) + B0015 (Terminator)
creator: Lovelace Soirez
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2127
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is simply the AIP sensor infrastructure ([http://partsregistry.org/wiki/index.php/Part:BBa_I746001 I746001]) from the agr system with a standard registry terminator ([http://partsregistry.org/wiki/index.php/Part:BBa_B0015 B0015]) downstream. The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. This part contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a SaII site.
mutable notes: N/A
mutable provenance: The original sequences for agrC and agrA were taken from the S. aureus Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_I746100
sequence annotation: BBa_I746100
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I746101_sequence/1

BBa_B0012
type: Component
persistent identity: component1952631
display id: component1952631
version: 1
top level: BBa_I746101
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1952629
display id: component1952629
version: 1
top level: BBa_I746101
access: public
definition: BBa_B0010

BBa_I746100
type: Component
persistent identity: component1952628
display id: component1952628
version: 1
top level: BBa_I746101
access: public
definition: https://synbiohub.org/public/igem/BBa_I746100/1

BBa_I746100
type: SequenceAnnotation
persistent identity: annotation1952628
display id: annotation1952628
version: 1
top level: BBa_I746101
location: https://synbiohub.org/public/igem/BBa_I746101/annotation1952628/range1952628/1
component: BBa_I746100

https://synbiohub.org/public/igem/BBa_I746101/annotation1952628/range1952628/1
type: Range
persistent identity: range1952628
display id: range1952628
version: 1
direction: direction/reverse
top level: BBa_I746101
start: 1
end: 1920
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1952631
display id: annotation1952631
version: 1
top level: BBa_I746101
location: https://synbiohub.org/public/igem/BBa_I746101/annotation1952631/range1952631/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746101/annotation1952631/range1952631/1
type: Range
persistent identity: range1952631
display id: range1952631
version: 1
direction: direction/forward
top level: BBa_I746101
start: 2017
end: 2057
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1952629
display id: annotation1952629
version: 1
top level: BBa_I746101
location: https://synbiohub.org/public/igem/BBa_I746101/annotation1952629/range1952629/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746101/annotation1952629/range1952629/1
type: Range
persistent identity: range1952629
display id: range1952629
version: 1
direction: direction/forward
top level: BBa_I746101
start: 1929
end: 2008
orientation: inline

BBa_K137076
type: ComponentDefinition
persistent identity: BBa K137076
display id: BBa_K137076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL inducible production of hydrogen peroxide
creator: Doug Tischer
created: 2008-08-08T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part produces pyruvate oxidase (K137069) in response to detecting acylhomoserine lactones.
mutable notes: No.
mutable provenance: The luxR reciever, RBS and double terminator are all from the registry. Pyruvate oxidase (spxB) is from a genomic PCR from Streptococcus pnumoniae R6.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_F2622
component: BBa_K137069
component: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence annotation: BBa_K137069
sequence annotation: BBa_F2622
sequence: https://synbiohub.org/public/igem/BBa_K137076_sequence/1

BBa_B0015
type: Component
persistent identity: component2281790
display id: component2281790
version: 1
top level: BBa_K137076
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_F2622
type: Component
persistent identity: component2281779
display id: component2281779
version: 1
top level: BBa_K137076
access: public
definition: BBa_F2622

BBa_K137069
type: Component
persistent identity: component2281783
display id: component2281783
version: 1
top level: BBa_K137076
access: public
definition: https://synbiohub.org/public/igem/BBa_K137069/1

BBa_B0034
type: Component
persistent identity: component2281781
display id: component2281781
version: 1
top level: BBa_K137076
access: public
definition: BBa_B0034

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2281790
display id: annotation2281790
version: 1
top level: BBa_K137076
location: https://synbiohub.org/public/igem/BBa_K137076/annotation2281790/range2281790/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K137076/annotation2281790/range2281790/1
type: Range
persistent identity: range2281790
display id: range2281790
version: 1
direction: direction/reverse
top level: BBa_K137076
start: 2876
end: 3004
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2281781
display id: annotation2281781
version: 1
top level: BBa_K137076
location: https://synbiohub.org/public/igem/BBa_K137076/annotation2281781/range2281781/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137076/annotation2281781/range2281781/1
type: Range
persistent identity: range2281781
display id: range2281781
version: 1
direction: direction/reverse
top level: BBa_K137076
start: 1071
end: 1082
orientation: inline

BBa_K137069
type: SequenceAnnotation
persistent identity: annotation2281783
display id: annotation2281783
version: 1
top level: BBa_K137076
location: https://synbiohub.org/public/igem/BBa_K137076/annotation2281783/range2281783/1
component: BBa_K137069

https://synbiohub.org/public/igem/BBa_K137076/annotation2281783/range2281783/1
type: Range
persistent identity: range2281783
display id: range2281783
version: 1
direction: direction/reverse
top level: BBa_K137076
start: 1089
end: 2867
orientation: inline

BBa_F2622
type: SequenceAnnotation
persistent identity: annotation2281779
display id: annotation2281779
version: 1
top level: BBa_K137076
location: https://synbiohub.org/public/igem/BBa_K137076/annotation2281779/range2281779/1
component: BBa_F2622

https://synbiohub.org/public/igem/BBa_K137076/annotation2281779/range2281779/1
type: Range
persistent identity: range2281779
display id: range2281779
version: 1
direction: direction/reverse
top level: BBa_K137076
start: 1
end: 1062
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa K592101
display id: BBa_K592101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Yellow Fluorescent Protein (YFP)
creator: Erik Lundin
created: 2011-09-20T11:00:00Z
modified: 2016-01-25T04:47:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 4206
owner id: 9827
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yellow Fluorescent Protein derived from A. victoria GFP. This fluorescent protein is cloned from the pZE12-YFP plasmid used by Elowitz (see reference). The original sequence was made by the The Yeast Resource Center (YRC) based at the University of Washington in Seattle, Washington.
mutable notes: This fluorescent protein was cloned from the pZE12-YFP plasmid used by Elowitz (see reference).
mutable provenance: Elowitz, M. B., A. J. Levine, et al. (2002). "Stochastic gene expression in a single cell." Science 297(5584): 1183-6.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: YFP
sequence: https://synbiohub.org/public/igem/BBa_K592101_sequence/1

YFP
type: SequenceAnnotation
persistent identity: annotation2137506
display id: annotation2137506
version: 1
top level: YFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592101/annotation2137506/range2137506/1

https://synbiohub.org/public/igem/BBa_K592101/annotation2137506/range2137506/1
type: Range
persistent identity: range2137506
display id: range2137506
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 714
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0036
display id: BBa_E0036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0036_sequence/1

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (PenI regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
mutable notes: extends slightly past -50
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: dimer left half
sequence annotation: PenI
sequence annotation: -10
sequence annotation: -35
sequence annotation: dimer right half
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
direction: direction/forward
top level: PenI
start: 31
end: 53
orientation: inline

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
direction: direction/forward
top level: PenI
start: 1
end: 77
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
direction: direction/forward
top level: PenI
start: 46
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
direction: direction/forward
top level: PenI
start: 23
end: 28
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
direction: direction/forward
top level: PenI
start: 55
end: 76
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0083
display id: BBa_R0083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
mutable notes: The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown. The a at base 41 is mutated to t to avoid XbaI site. Speculation that this was a previous assembly site.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: linker
sequence annotation: -10
sequence annotation: A
sequence annotation: C1 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0083_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation301224
display id: annotation301224
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1
type: Range
persistent identity: range301224
display id: range301224
version: 1
direction: direction/forward
top level: OmpR
start: 45
end: 50
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation301223
display id: annotation301223
version: 1
top level: OmpR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1
type: Range
persistent identity: range301223
display id: range301223
version: 1
direction: direction/forward
top level: OmpR
start: 37
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301226
display id: annotation301226
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1
type: Range
persistent identity: range301226
display id: range301226
version: 1
direction: direction/forward
top level: OmpR
start: 68
end: 73
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation306881
display id: annotation306881
version: 1
top level: OmpR
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1

https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1
type: Range
persistent identity: range306881
display id: range306881
version: 1
direction: direction/forward
top level: OmpR
start: 41
end: 41
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301221
display id: annotation301221
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1
type: Range
persistent identity: range301221
display id: range301221
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: N/A
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_K115017
type: ComponentDefinition
persistent identity: BBa K115017
display id: BBa_K115017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA thermometer (ROSE 32??C)
creator: Bastiaan van den Berg
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:09:28Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: An RNA thermometer that theoretically switches on translation at 32 degrees Celcius and switched of translation below that temperature. Still to be tested.
mutable notes: a lot, will be added soon.
mutable provenance: Based on ROSE RNA thermometer sequences from Rfam database.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: SD
sequence: https://synbiohub.org/public/igem/BBa_K115017_sequence/1

SD
type: SequenceAnnotation
persistent identity: annotation1972920
display id: annotation1972920
version: 1
top level: BBa_K115017
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K115017/annotation1972920/range1972920/1

https://synbiohub.org/public/igem/BBa_K115017/annotation1972920/range1972920/1
type: Range
persistent identity: range1972920
display id: range1972920
version: 1
direction: direction/forward
top level: BBa_K115017
start: 77
end: 82
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pL)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
mutable provenance: V. fischeri.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence annotation: Putative LuxR/HSL
sequence annotation: BBa_R0063
sequence annotation: LuxR/HSL
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
direction: direction/forward
top level: lux pL
start: 89
end: 94
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
direction: direction/forward
top level: lux pL
start: 115
end: 122
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
direction: direction/forward
top level: lux pL
start: 130
end: 149
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 151
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
direction: direction/forward
top level: lux pL
start: 1
end: 20
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
direction: direction/forward
top level: lux pL
start: 128
end: 128
orientation: inline

BBa_K145302
type: ComponentDefinition
persistent identity: BBa K145302
display id: BBa_K145302
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxI generator controlled by key/lock
creator: Nathalie Busschaert
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device can generate luxI. luxI is an enzym that can catalyse the production of HSL. When no key is present, there will be no lacI and therefore luxI will be produced. However, when key is present, lacI will be produced and this wil repress the production of luxI.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K145301
component: BBa_K145254
sequence annotation: BBa_K145254
sequence annotation: BBa_K145301
sequence: https://synbiohub.org/public/igem/BBa_K145302_sequence/1

BBa_K145301
type: Component
persistent identity: component2282339
display id: component2282339
version: 1
top level: BBa_K145302
access: public
definition: https://synbiohub.org/public/igem/BBa_K145301/1

BBa_K145254
type: Component
persistent identity: component2282359
display id: component2282359
version: 1
top level: BBa_K145302
access: public
definition: https://synbiohub.org/public/igem/BBa_K145254/1

BBa_K145254
type: SequenceAnnotation
persistent identity: annotation2282359
display id: annotation2282359
version: 1
top level: BBa_K145302
location: https://synbiohub.org/public/igem/BBa_K145302/annotation2282359/range2282359/1
component: BBa_K145254

https://synbiohub.org/public/igem/BBa_K145302/annotation2282359/range2282359/1
type: Range
persistent identity: range2282359
display id: range2282359
version: 1
direction: direction/reverse
top level: BBa_K145302
start: 1391
end: 2252
orientation: inline

BBa_K145301
type: SequenceAnnotation
persistent identity: annotation2282339
display id: annotation2282339
version: 1
top level: BBa_K145302
location: https://synbiohub.org/public/igem/BBa_K145302/annotation2282339/range2282339/1
component: BBa_K145301

https://synbiohub.org/public/igem/BBa_K145302/annotation2282339/range2282339/1
type: Range
persistent identity: range2282339
display id: range2282339
version: 1
direction: direction/reverse
top level: BBa_K145302
start: 1
end: 1382
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0077
display id: BBa_R0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated, RBS+)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CinR (BBa_C0077) in complex with O3-C14:1-HSL (from CinI, BBa_C0076) binds to this promoter and activates transcription. This promoter contains the RBS: TGGAGG
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: the last 5 bases (gctaa) removed to allow for the correct spacing between the RBS (TGGAGG) and the start of the downstream gene after biobricks splicing.
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: stem_loop
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: embedded RBS
sequence: https://synbiohub.org/public/igem/BBa_R0077_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation301204
display id: annotation301204
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1
type: Range
persistent identity: range301204
display id: range301204
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation301371
display id: annotation301371
version: 1
top level: cinR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1
type: Range
persistent identity: range301371
display id: range301371
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

embedded RBS
type: SequenceAnnotation
persistent identity: annotation301150
display id: annotation301150
version: 1
top level: cinR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1
type: Range
persistent identity: range301150
display id: range301150
version: 1
direction: direction/forward
top level: cinR
start: 226
end: 231
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301205
display id: annotation301205
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1
type: Range
persistent identity: range301205
display id: range301205
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_E1010
component: BBa_B0010
component: BBa_R0010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: BBa_B0034

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: BBa_B0010

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_K1150004
type: ComponentDefinition
persistent identity: BBa K1150004
display id: BBa_K1150004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PhyB
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: PhyB is ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PhyB
sequence: https://synbiohub.org/public/igem/BBa_K1150004_sequence/1

PhyB
type: SequenceAnnotation
persistent identity: annotation2347614
display id: annotation2347614
version: 1
top level: BBa_K1150004
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1150004/annotation2347614/range2347614/1

https://synbiohub.org/public/igem/BBa_K1150004/annotation2347614/range2347614/1
type: Range
persistent identity: range2347614
display id: range2347614
version: 1
direction: direction/forward
top level: BBa_K1150004
start: 1
end: 1950
orientation: inline

BBa_K1150003
type: ComponentDefinition
persistent identity: BBa K1150003
display id: BBa_K1150003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: G9a
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: G9a is ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: G9a
sequence: https://synbiohub.org/public/igem/BBa_K1150003_sequence/1

G9a
type: SequenceAnnotation
persistent identity: annotation2347615
display id: annotation2347615
version: 1
top level: BBa_K1150003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1150003/annotation2347615/range2347615/1

https://synbiohub.org/public/igem/BBa_K1150003/annotation2347615/range2347615/1
type: Range
persistent identity: range2347615
display id: range2347615
version: 1
direction: direction/forward
top level: BBa_K1150003
start: 1
end: 840
orientation: inline

BBa_K1150037
type: ComponentDefinition
persistent identity: BBa K1150037
display id: BBa_K1150037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: uniCAS RNaimer to target VEGF2
creator: Ilona Blank
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ..
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1150037_sequence/1

BBa_I759014
type: ComponentDefinition
persistent identity: BBa I759014
display id: BBa_I759014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cis2-repressed, tet-regulated YFP
creator: Kat Pak
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: true
experience: experience/None
group u list: _115_
m user id: 0
owner id: 1834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has been shown to repress the expression of YFP via a cis-repressive element called cr2 which sequesters the ribosome binding site of YFP.
mutable provenance: The cr element is a synthetic oligo which has been inserted into part I759009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S03724
component: BBa_I759034
sequence annotation: BBa_S03724
sequence annotation: BBa_I759034
sequence: https://synbiohub.org/public/igem/BBa_I759014_sequence/1

BBa_S03724
type: Component
persistent identity: component2271118
display id: component2271118
version: 1
top level: BBa_I759014
access: public
definition: https://synbiohub.org/public/igem/BBa_S03724/1

BBa_I759034
type: Component
persistent identity: component2271095
display id: component2271095
version: 1
top level: BBa_I759014
access: public
definition: https://synbiohub.org/public/igem/BBa_I759034/1

BBa_S03724
type: SequenceAnnotation
persistent identity: annotation2271118
display id: annotation2271118
version: 1
top level: BBa_I759014
location: https://synbiohub.org/public/igem/BBa_I759014/annotation2271118/range2271118/1
component: BBa_S03724

https://synbiohub.org/public/igem/BBa_I759014/annotation2271118/range2271118/1
type: Range
persistent identity: range2271118
display id: range2271118
version: 1
direction: direction/reverse
top level: BBa_I759014
start: 838
end: 1857
orientation: inline

BBa_I759034
type: SequenceAnnotation
persistent identity: annotation2271095
display id: annotation2271095
version: 1
top level: BBa_I759014
location: https://synbiohub.org/public/igem/BBa_I759014/annotation2271095/range2271095/1
component: BBa_I759034

https://synbiohub.org/public/igem/BBa_I759014/annotation2271095/range2271095/1
type: Range
persistent identity: range2271095
display id: range2271095
version: 1
direction: direction/reverse
top level: BBa_I759014
start: 1
end: 829
orientation: inline

SgsE | CFP
type: ComponentDefinition
persistent identity: BBa K525306
display id: BBa_K525306
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SgsE and mCerulean
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T09:40:40Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and mCerulean
mutable notes: Fusion Protein of BBa_K525303 and BBa_J18930
mutable provenance: Fusion Protein of S-Layer SgsE and mCerulean
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SgsE domain
sequence annotation: J18930
sequence annotation: mCerulean
sequence annotation: B0034
sequence annotation: K525301
sequence annotation: T7 promoter
sequence annotation: TAA
sequence annotation: AgeI
sequence annotation: RBS
sequence annotation: NgoMIV
sequence annotation: ATG
sequence annotation: lac operator
sequence annotation: Freiburg Scar
sequence: https://synbiohub.org/public/igem/BBa_K525306_sequence/1

SgsE domain
type: SequenceAnnotation
persistent identity: annotation2128600
display id: annotation2128600
version: 1
top level: SgsE | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128600/range2128600/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128600/range2128600/1
type: Range
persistent identity: range2128600
display id: range2128600
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 73
end: 2400
orientation: inline

J18930
type: SequenceAnnotation
persistent identity: annotation2128602
display id: annotation2128602
version: 1
top level: SgsE | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128602/range2128602/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128602/range2128602/1
type: Range
persistent identity: range2128602
display id: range2128602
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 2406
end: 3129
orientation: inline

mCerulean
type: SequenceAnnotation
persistent identity: annotation2128603
display id: annotation2128603
version: 1
top level: SgsE | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128603/range2128603/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128603/range2128603/1
type: Range
persistent identity: range2128603
display id: range2128603
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 2406
end: 3129
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2128588
display id: annotation2128588
version: 1
top level: SgsE | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128588/range2128588/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128588/range2128588/1
type: Range
persistent identity: range2128588
display id: range2128588
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 53
end: 64
orientation: inline

K525301
type: SequenceAnnotation
persistent identity: annotation2128599
display id: annotation2128599
version: 1
top level: SgsE | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128599/range2128599/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128599/range2128599/1
type: Range
persistent identity: range2128599
display id: range2128599
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 73
end: 2400
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2128585
display id: annotation2128585
version: 1
top level: SgsE | CFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128585/range2128585/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128585/range2128585/1
type: Range
persistent identity: range2128585
display id: range2128585
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 1
end: 20
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2128607
display id: annotation2128607
version: 1
top level: SgsE | CFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128607/range2128607/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128607/range2128607/1
type: Range
persistent identity: range2128607
display id: range2128607
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 3126
end: 3129
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2128605
display id: annotation2128605
version: 1
top level: SgsE | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128605/range2128605/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128605/range2128605/1
type: Range
persistent identity: range2128605
display id: range2128605
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 3121
end: 3126
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2128598
display id: annotation2128598
version: 1
top level: SgsE | CFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128598/range2128598/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128598/range2128598/1
type: Range
persistent identity: range2128598
display id: range2128598
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 56
end: 59
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2128604
display id: annotation2128604
version: 1
top level: SgsE | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128604/range2128604/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128604/range2128604/1
type: Range
persistent identity: range2128604
display id: range2128604
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 76
end: 81
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2128606
display id: annotation2128606
version: 1
top level: SgsE | CFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128606/range2128606/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128606/range2128606/1
type: Range
persistent identity: range2128606
display id: range2128606
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 73
end: 75
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2128587
display id: annotation2128587
version: 1
top level: SgsE | CFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128587/range2128587/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128587/range2128587/1
type: Range
persistent identity: range2128587
display id: range2128587
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 20
end: 44
orientation: inline

Freiburg Scar
type: SequenceAnnotation
persistent identity: annotation2128601
display id: annotation2128601
version: 1
top level: SgsE | CFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525306/annotation2128601/range2128601/1

https://synbiohub.org/public/igem/BBa_K525306/annotation2128601/range2128601/1
type: Range
persistent identity: range2128601
display id: range2128601
version: 1
direction: direction/forward
top level: SgsE | CFP
start: 2401
end: 2406
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated)
creator: Drew Endy
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: stem_loop
sequence annotation: unidentified/uncharacterized CinR dependent
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

BBa_J45299
type: ComponentDefinition
persistent identity: BBa J45299
display id: BBa_J45299
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PchA & PchB enzyme generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is an intermediate to the protein generator BBa_45300.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J45017
component: BBa_B0010
component: BBa_B0030
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_J45017
sequence: https://synbiohub.org/public/igem/BBa_J45299_sequence/1

BBa_J45017
type: Component
persistent identity: component1960923
display id: component1960923
version: 1
top level: BBa_J45299
access: public
definition: https://synbiohub.org/public/igem/BBa_J45017/1

BBa_B0010
type: Component
persistent identity: component1960924
display id: component1960924
version: 1
top level: BBa_J45299
access: public
definition: BBa_B0010

BBa_B0030
type: Component
persistent identity: component1960916
display id: component1960916
version: 1
top level: BBa_J45299
access: public
definition: BBa_B0030

BBa_B0012
type: Component
persistent identity: component1960926
display id: component1960926
version: 1
top level: BBa_J45299
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1960916
display id: annotation1960916
version: 1
top level: BBa_J45299
location: https://synbiohub.org/public/igem/BBa_J45299/annotation1960916/range1960916/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45299/annotation1960916/range1960916/1
type: Range
persistent identity: range1960916
display id: range1960916
version: 1
direction: direction/forward
top level: BBa_J45299
start: 1
end: 15
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960926
display id: annotation1960926
version: 1
top level: BBa_J45299
location: https://synbiohub.org/public/igem/BBa_J45299/annotation1960926/range1960926/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45299/annotation1960926/range1960926/1
type: Range
persistent identity: range1960926
display id: range1960926
version: 1
direction: direction/forward
top level: BBa_J45299
start: 1854
end: 1894
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960924
display id: annotation1960924
version: 1
top level: BBa_J45299
location: https://synbiohub.org/public/igem/BBa_J45299/annotation1960924/range1960924/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45299/annotation1960924/range1960924/1
type: Range
persistent identity: range1960924
display id: range1960924
version: 1
direction: direction/forward
top level: BBa_J45299
start: 1766
end: 1845
orientation: inline

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation1960923
display id: annotation1960923
version: 1
top level: BBa_J45299
location: https://synbiohub.org/public/igem/BBa_J45299/annotation1960923/range1960923/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_J45299/annotation1960923/range1960923/1
type: Range
persistent identity: range1960923
display id: range1960923
version: 1
direction: direction/reverse
top level: BBa_J45299
start: 22
end: 1757
orientation: inline

BBa_K648013
type: ComponentDefinition
persistent identity: BBa K648013
display id: BBa_K648013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP with Standard 25 Prefix/Suffix
creator: Jim Rose
created: 2011-07-03T11:00:00Z
modified: 2016-01-25T02:32:21Z
discontinued: false
dominant: false
group u list: _825_
m user id: 4206
owner id: 9871
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the standard GFP protein reporter (E0040) cloned with the prefix/suffix required for standard 25 assembly. It can easily be used to create fusion proteins through this method.
mutable notes: This part contains the AgeI and NgoMIV sites as part of it's prefix/suffix that allows it to be used in standard 25 assembly. It was synthesized through PCR oligo synthesis methods using the following primers: Forward (56.88 degrees) TATTGAATTCGCGGCCGCTTCTAGATGGCCGGCCGTAAAGGAGAAGAACTTTTC Reverse (56.95 degrees) AATACTGCAGCGGCCGCTACTAGTATTAACCGGTCTTGTCGTCATCATCTTTATAAT
mutable provenance: This part is similar to the part BBa_E0040 commonly used as a reporter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: misc
sequence: https://synbiohub.org/public/igem/BBa_K648013_sequence/1

misc
type: SequenceAnnotation
persistent identity: annotation2122827
display id: annotation2122827
version: 1
top level: BBa_K648013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K648013/annotation2122827/range2122827/1

https://synbiohub.org/public/igem/BBa_K648013/annotation2122827/range2122827/1
type: Range
persistent identity: range2122827
display id: range2122827
version: 1
direction: direction/forward
top level: BBa_K648013
start: 1
end: 735
orientation: inline

TAL
type: ComponentDefinition
persistent identity: BBa K1033000
display id: BBa_K1033000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tyrosine ammonia-lyase (TAL) with RBS
creator: Karl Holdar, Hampus Elofsson, Emil Marklund, Kristoffer Lundmark, Marcus Hong, Lovisa Pettersson, Ken Braech-Andersen, Theodor L??we
created: 2013-08-14T11:00:00Z
modified: 2015-05-08T01:08:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 18317
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: tyrosine ammonia-lyase (TAL) is an enzyme which catalyzes the formation of p-coumaric acid (aka p-hyroxycinnamic acid) from tyrosine. It belongs to the family of ammonia-lyases, enzymes that catalyze the deamination of amino acids [1]. P-coumaric acid is an important precursor in many metabolic pathways. TAL also has a secondary function as a phenylalanine ammonia-lyase (PAL) which catalyses the formation of cinnamic acid. The wild type RsTAL has a preference for tyrosine, however the mutations Met4 -> Ile, Ile325 -> Val and Val409 -> Met, can shift this preference to phenylalanine [2]. 1.Zhixiong Xue, Michael McCluskey, Keith Cantera, F. Sima Sariaslani, Lixuan Huang (2007) Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroides. J Ind Microbiol Biotechnol 34:599-604 2. J.A. Kyndt, T.E. Meyer, M.A Cusanovich, J.J. Van Beeumen (2002) Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow protein. FEBS Letters 512 240-244
mutable notes: Earlier in iGEM eucaryot TAL has been used, however we used bacterial TAL for better compatibility with E.coli.
mutable provenance: Rhodobacter sphaeroides
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: B0034
sequence annotation: Tyrosine amonia lyase
sequence: https://synbiohub.org/public/igem/BBa_K1033000_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2366524
display id: annotation2366524
version: 1
top level: TAL
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1033000/annotation2366524/range2366524/1

https://synbiohub.org/public/igem/BBa_K1033000/annotation2366524/range2366524/1
type: Range
persistent identity: range2366524
display id: range2366524
version: 1
direction: direction/forward
top level: TAL
start: 4
end: 13
orientation: inline

Tyrosine amonia lyase
type: SequenceAnnotation
persistent identity: annotation2366489
display id: annotation2366489
version: 1
top level: TAL
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033000/annotation2366489/range2366489/1

https://synbiohub.org/public/igem/BBa_K1033000/annotation2366489/range2366489/1
type: Range
persistent identity: range2366489
display id: range2366489
version: 1
direction: direction/forward
top level: TAL
start: 22
end: 1596
orientation: inline

BBa_J52039
type: ComponentDefinition
persistent identity: BBa J52039
display id: BBa_J52039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV + Rluc-linker-PEST191
creator: Monika Ciglic
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is made of constitutive promotor CMV and reporter Rluc with degradation tag PEST191.
mutable notes: This compositive part is cloned in vector BBa_J52017 (we have made it by ligating eucaryotic terminator in BioBrick vector pSB1AK3).
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J52034
component: BBa_J52012
sequence annotation: BBa_J52034
sequence annotation: BBa_J52012
sequence: https://synbiohub.org/public/igem/BBa_J52039_sequence/1

BBa_J52034
type: Component
persistent identity: component1903156
display id: component1903156
version: 1
top level: BBa_J52039
access: public
definition: https://synbiohub.org/public/igem/BBa_J52034/1

BBa_J52012
type: Component
persistent identity: component1903161
display id: component1903161
version: 1
top level: BBa_J52039
access: public
definition: https://synbiohub.org/public/igem/BBa_J52012/1

BBa_J52034
type: SequenceAnnotation
persistent identity: annotation1903156
display id: annotation1903156
version: 1
top level: BBa_J52039
location: https://synbiohub.org/public/igem/BBa_J52039/annotation1903156/range1903156/1
component: BBa_J52034

https://synbiohub.org/public/igem/BBa_J52039/annotation1903156/range1903156/1
type: Range
persistent identity: range1903156
display id: range1903156
version: 1
direction: direction/reverse
top level: BBa_J52039
start: 1
end: 654
orientation: inline

BBa_J52012
type: SequenceAnnotation
persistent identity: annotation1903161
display id: annotation1903161
version: 1
top level: BBa_J52039
location: https://synbiohub.org/public/igem/BBa_J52039/annotation1903161/range1903161/1
component: BBa_J52012

https://synbiohub.org/public/igem/BBa_J52039/annotation1903161/range1903161/1
type: Range
persistent identity: range1903161
display id: range1903161
version: 1
direction: direction/reverse
top level: BBa_J52039
start: 661
end: 2097
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R1051
display id: BBa_R1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Modified to comply with BBa_S0001: TTGACA-17N-GATACT Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: OR2
sequence annotation: OR1
sequence annotation: BBa_R1051
sequence: https://synbiohub.org/public/igem/BBa_R1051_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2078
display id: annotation2078
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
type: Range
persistent identity: range2078
display id: range2078
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2075
display id: annotation2075
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
type: Range
persistent identity: range2075
display id: range2075
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2077
display id: annotation2077
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
type: Range
persistent identity: range2077
display id: range2077
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2076
display id: annotation2076
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
type: Range
persistent identity: range2076
display id: range2076
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation7074
display id: annotation7074
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1

https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
type: Range
persistent identity: range7074
display id: range7074
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

BBa_I759017
type: ComponentDefinition
persistent identity: BBa I759017
display id: BBa_I759017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cis5-repressed, tet-regulated YFP
creator: Kat Pak
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: true
experience: experience/None
group u list: _115_
m user id: 0
owner id: 1834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is designed to repress the expression of YFP via a cis-repressive element called cr5 which sequesters the ribosome binding site of YFP.
mutable provenance: The cr element is a synthetic oligo which has been inserted into part I759009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S03724
component: BBa_I759040
sequence annotation: BBa_S03724
sequence annotation: BBa_I759040
sequence: https://synbiohub.org/public/igem/BBa_I759017_sequence/1

BBa_S03724
type: Component
persistent identity: component2271199
display id: component2271199
version: 1
top level: BBa_I759017
access: public
definition: https://synbiohub.org/public/igem/BBa_S03724/1

BBa_I759040
type: Component
persistent identity: component2271176
display id: component2271176
version: 1
top level: BBa_I759017
access: public
definition: https://synbiohub.org/public/igem/BBa_I759040/1

BBa_S03724
type: SequenceAnnotation
persistent identity: annotation2271199
display id: annotation2271199
version: 1
top level: BBa_I759017
location: https://synbiohub.org/public/igem/BBa_I759017/annotation2271199/range2271199/1
component: BBa_S03724

https://synbiohub.org/public/igem/BBa_I759017/annotation2271199/range2271199/1
type: Range
persistent identity: range2271199
display id: range2271199
version: 1
direction: direction/reverse
top level: BBa_I759017
start: 842
end: 1861
orientation: inline

BBa_I759040
type: SequenceAnnotation
persistent identity: annotation2271176
display id: annotation2271176
version: 1
top level: BBa_I759017
location: https://synbiohub.org/public/igem/BBa_I759017/annotation2271176/range2271176/1
component: BBa_I759040

https://synbiohub.org/public/igem/BBa_I759017/annotation2271176/range2271176/1
type: Range
persistent identity: range2271176
display id: range2271176
version: 1
direction: direction/reverse
top level: BBa_I759017
start: 1
end: 833
orientation: inline

BBa_K115002
type: ComponentDefinition
persistent identity: BBa K115002
display id: BBa_K115002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA thermometer (FourU)
creator: Bastiaan van den Berg
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Thermo sensitive small RNA (ROSE structure) which can be used as a RNA regulator.
mutable notes: Part of the sequence is altered because of the scar...
mutable provenance: Salmonella Enterica Tyhpy (CP000886.1)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: stem_loop
sequence annotation: scar adaptation
sequence annotation: rbs
sequence annotation: predicted stem-loop extending to one position before start codon
sequence: https://synbiohub.org/public/igem/BBa_K115002_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1966930
display id: annotation1966930
version: 1
top level: BBa_K115002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1

https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1
type: Range
persistent identity: range1966930
display id: range1966930
version: 1
direction: direction/forward
top level: BBa_K115002
start: 1
end: 18
orientation: inline

scar adaptation
type: SequenceAnnotation
persistent identity: annotation1966899
display id: annotation1966899
version: 1
top level: BBa_K115002
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1

https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1
type: Range
persistent identity: range1966899
display id: range1966899
version: 1
direction: direction/forward
top level: BBa_K115002
start: 23
end: 28
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1966897
display id: annotation1966897
version: 1
top level: BBa_K115002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1

https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1
type: Range
persistent identity: range1966897
display id: range1966897
version: 1
direction: direction/forward
top level: BBa_K115002
start: 47
end: 52
orientation: inline

predicted stem-loop extending to one position before start codon
type: SequenceAnnotation
persistent identity: annotation1966931
display id: annotation1966931
version: 1
top level: BBa_K115002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1

https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1
type: Range
persistent identity: range1966931
display id: range1966931
version: 1
direction: direction/forward
top level: BBa_K115002
start: 24
end: 52
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
mutable provenance: AF196486 from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: 2
sequence annotation: LVA
sequence annotation: BBa_C0060
sequence annotation: Help:Barcodes
sequence annotation: aiiA
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 3
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
direction: direction/forward
top level: aiiA
start: 784
end: 789
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
direction: direction/forward
top level: aiiA
start: 751
end: 783
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 789
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
direction: direction/forward
top level: aiiA
start: 790
end: 814
orientation: inline

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 750
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (AraC regulated)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: araO1
sequence annotation: -35
sequence annotation: c-amp1
sequence annotation: c-amp2
sequence annotation: ara1 and ara2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
direction: direction/forward
top level: AraC
start: 6
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
direction: direction/forward
top level: AraC
start: 113
end: 118
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
direction: direction/forward
top level: AraC
start: 43
end: 72
orientation: inline

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
direction: direction/forward
top level: AraC
start: 4
end: 29
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
direction: direction/forward
top level: AraC
start: 73
end: 101
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
direction: direction/forward
top level: AraC
start: 136
end: 141
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: CFP (LVA)
sequence annotation: SsrA
sequence annotation: A
sequence annotation: 2
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
direction: direction/forward
top level: ECFP
start: 1
end: 762
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
direction: direction/forward
top level: ECFP
start: 719
end: 756
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
direction: direction/forward
top level: ECFP
start: 69
end: 69
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
direction: direction/forward
top level: ECFP
start: 757
end: 762
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
direction: direction/forward
top level: ECFP
start: 1
end: 762
orientation: inline

BBa_I13001
type: ComponentDefinition
persistent identity: BBa I13001
display id: BBa_I13001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS B0030 w/ YFP (-LVA) TT
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0030. E0030. B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0030
component: BBa_B0010
component: BBa_E0030
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I13001_sequence/1

BBa_B0030
type: Component
persistent identity: component943428
display id: component943428
version: 1
top level: BBa_I13001
access: public
definition: BBa_B0030

BBa_B0010
type: Component
persistent identity: component943436
display id: component943436
version: 1
top level: BBa_I13001
access: public
definition: BBa_B0010

BBa_E0030
type: Component
persistent identity: component943431
display id: component943431
version: 1
top level: BBa_I13001
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0012
type: Component
persistent identity: component943446
display id: component943446
version: 1
top level: BBa_I13001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943436
display id: annotation943436
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943436/range943436/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13001/annotation943436/range943436/1
type: Range
persistent identity: range943436
display id: range943436
version: 1
direction: direction/forward
top level: BBa_I13001
start: 753
end: 832
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943431
display id: annotation943431
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943431/range943431/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13001/annotation943431/range943431/1
type: Range
persistent identity: range943431
display id: range943431
version: 1
direction: direction/forward
top level: BBa_I13001
start: 22
end: 744
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943446
display id: annotation943446
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943446/range943446/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13001/annotation943446/range943446/1
type: Range
persistent identity: range943446
display id: range943446
version: 1
direction: direction/forward
top level: BBa_I13001
start: 841
end: 881
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation943428
display id: annotation943428
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943428/range943428/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I13001/annotation943428/range943428/1
type: Range
persistent identity: range943428
display id: range943428
version: 1
direction: direction/forward
top level: BBa_I13001
start: 1
end: 15
orientation: inline

BBa_J61101
type: ComponentDefinition
persistent identity: BBa J61101
display id: BBa_J61101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ribosome Binding Site Family Member
creator: John Anderson
created: 2007-01-28T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable description: fix
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61101_sequence/1

BBa_K747095
type: ComponentDefinition
persistent identity: BBa K747095
display id: BBa_K747095
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TT6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TT 6
sequence: https://synbiohub.org/public/igem/BBa_K747095_sequence/1

TT 6
type: SequenceAnnotation
persistent identity: annotation2197073
display id: annotation2197073
version: 1
top level: BBa_K747095
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747095/annotation2197073/range2197073/1

https://synbiohub.org/public/igem/BBa_K747095/annotation2197073/range2197073/1
type: Range
persistent identity: range2197073
display id: range2197073
version: 1
direction: direction/forward
top level: BBa_K747095
start: 14
end: 211
orientation: inline

BBa_J23107
type: ComponentDefinition
persistent identity: BBa J23107
display id: BBa_J23107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23107_sequence/1

mRFP1
type: ComponentDefinition
persistent identity: BBa I13502
display id: BBa_I13502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_I13502_sequence/1

BBa_B0034
type: Component
persistent identity: component1505038
display id: component1505038
version: 1
top level: mRFP1
access: public
definition: BBa_B0034

BBa_E1010
type: Component
persistent identity: component1505045
display id: component1505045
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505038
display id: annotation1505038
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13502/annotation1505038/range1505038/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13502/annotation1505038/range1505038/1
type: Range
persistent identity: range1505038
display id: range1505038
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505045
display id: annotation1505045
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13502/annotation1505045/range1505045/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13502/annotation1505045/range1505045/1
type: Range
persistent identity: range1505045
display id: range1505045
version: 1
direction: direction/forward
top level: mRFP1
start: 19
end: 699
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lambda cI and luxR regulated -- hybrid)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 cI
sequence annotation: -10
sequence annotation: Lux Box
sequence annotation: BBa_R0065
sequence annotation: -35
sequence annotation: lux p(R) start
sequence annotation: OR2 cI
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
direction: direction/forward
top level: cI+luxR
start: 81
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
direction: direction/forward
top level: cI+luxR
start: 94
end: 97
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
direction: direction/forward
top level: cI+luxR
start: 6
end: 25
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
direction: direction/forward
top level: cI+luxR
start: 1
end: 97
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
direction: direction/forward
top level: cI+luxR
start: 71
end: 76
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
direction: direction/forward
top level: cI+luxR
start: 58
end: 58
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
direction: direction/forward
top level: cI+luxR
start: 47
end: 52
orientation: inline

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
direction: direction/forward
top level: cI+luxR
start: 57
end: 73
orientation: inline

BBa_E0430
type: ComponentDefinition
persistent identity: BBa E0430
display id: BBa_E0430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
creator: Caitlin Conboy
created: 2004-03-15T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Standard YFP Output Device -LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0430_sequence/1

BBa_B0034
type: Component
persistent identity: component942885
display id: component942885
version: 1
top level: BBa_E0430
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component942892
display id: component942892
version: 1
top level: BBa_E0430
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component942902
display id: component942902
version: 1
top level: BBa_E0430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component942887
display id: component942887
version: 1
top level: BBa_E0430
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942885
display id: annotation942885
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942885/range942885/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0430/annotation942885/range942885/1
type: Range
persistent identity: range942885
display id: range942885
version: 1
direction: direction/forward
top level: BBa_E0430
start: 1
end: 12
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation942887
display id: annotation942887
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942887/range942887/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_E0430/annotation942887/range942887/1
type: Range
persistent identity: range942887
display id: range942887
version: 1
direction: direction/forward
top level: BBa_E0430
start: 19
end: 741
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942902
display id: annotation942902
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942902/range942902/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0430/annotation942902/range942902/1
type: Range
persistent identity: range942902
display id: range942902
version: 1
direction: direction/forward
top level: BBa_E0430
start: 838
end: 878
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942892
display id: annotation942892
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942892/range942892/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0430/annotation942892/range942892/1
type: Range
persistent identity: range942892
display id: range942892
version: 1
direction: direction/forward
top level: BBa_E0430
start: 750
end: 829
orientation: inline

BBa_I13500
type: ComponentDefinition
persistent identity: BBa I13500
display id: BBa_I13500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13500_sequence/1

BBa_B0034
type: Component
persistent identity: component1505000
display id: component1505000
version: 1
top level: BBa_I13500
access: public
definition: BBa_B0034

BBa_E0040
type: Component
persistent identity: component1505002
display id: component1505002
version: 1
top level: BBa_I13500
access: public
definition: GFP

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505000
display id: annotation1505000
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
type: Range
persistent identity: range1505000
display id: range1505000
version: 1
direction: direction/forward
top level: BBa_I13500
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505002
display id: annotation1505002
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
type: Range
persistent identity: range1505002
display id: range1505002
version: 1
direction: direction/forward
top level: BBa_I13500
start: 19
end: 738
orientation: inline

BBa_K115016
type: ComponentDefinition
persistent identity: BBa K115016
display id: BBa_K115016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA thermometer (ROSE 27??C)
creator: Bastiaan van den Berg
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:09:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: An RNA thermometer that theoretically switches on translation at 27 degrees Celcius and switched of translation below that temperature. Still to be tested.
mutable notes: a lot, will be added soon.
mutable provenance: Based on ROSE RNA thermometer sequences from Rfam database.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: SD
sequence: https://synbiohub.org/public/igem/BBa_K115016_sequence/1

SD
type: SequenceAnnotation
persistent identity: annotation1972919
display id: annotation1972919
version: 1
top level: BBa_K115016
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K115016/annotation1972919/range1972919/1

https://synbiohub.org/public/igem/BBa_K115016/annotation1972919/range1972919/1
type: Range
persistent identity: range1972919
display id: range1972919
version: 1
direction: direction/forward
top level: BBa_K115016
start: 77
end: 82
orientation: inline

BBa_I746391
type: ComponentDefinition
persistent identity: BBa I746391
display id: BBa_I746391
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phiR73 delta inducing PO promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the phiR73 delta activator on the PO promoter. Note that the I746352 does contain a RBS.
mutable notes: I746315 was put upstream of I746321
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746321
component: BBa_I746315
sequence annotation: BBa_I746315
sequence annotation: BBa_I746321
sequence: https://synbiohub.org/public/igem/BBa_I746391_sequence/1

BBa_I746321
type: Component
persistent identity: component2292073
display id: component2292073
version: 1
top level: BBa_I746391
access: public
definition: https://synbiohub.org/public/igem/BBa_I746321/1

BBa_I746315
type: Component
persistent identity: component2292060
display id: component2292060
version: 1
top level: BBa_I746391
access: public
definition: https://synbiohub.org/public/igem/BBa_I746315/1

BBa_I746315
type: SequenceAnnotation
persistent identity: annotation2292060
display id: annotation2292060
version: 1
top level: BBa_I746391
location: https://synbiohub.org/public/igem/BBa_I746391/annotation2292060/range2292060/1
component: BBa_I746315

https://synbiohub.org/public/igem/BBa_I746391/annotation2292060/range2292060/1
type: Range
persistent identity: range2292060
display id: range2292060
version: 1
direction: direction/reverse
top level: BBa_I746391
start: 1
end: 2351
orientation: inline

BBa_I746321
type: SequenceAnnotation
persistent identity: annotation2292073
display id: annotation2292073
version: 1
top level: BBa_I746391
location: https://synbiohub.org/public/igem/BBa_I746391/annotation2292073/range2292073/1
component: BBa_I746321

https://synbiohub.org/public/igem/BBa_I746391/annotation2292073/range2292073/1
type: Range
persistent identity: range2292073
display id: range2292073
version: 1
direction: direction/reverse
top level: BBa_I746391
start: 2360
end: 3334
orientation: inline

BBa_I759009
type: ComponentDefinition
persistent identity: BBa I759009
display id: BBa_I759009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tet-regulated YFP (Weak tetR promoter)
creator: Kat Pak
created: 2007-07-29T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _115_
m user id: 0
owner id: 1834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S03724
component: BBa_I759008
sequence annotation: BBa_S03724
sequence annotation: BBa_I759008
sequence: https://synbiohub.org/public/igem/BBa_I759009_sequence/1

BBa_S03724
type: Component
persistent identity: component2270789
display id: component2270789
version: 1
top level: BBa_I759009
access: public
definition: https://synbiohub.org/public/igem/BBa_S03724/1

BBa_I759008
type: Component
persistent identity: component2270766
display id: component2270766
version: 1
top level: BBa_I759009
access: public
definition: https://synbiohub.org/public/igem/BBa_I759008/1

BBa_S03724
type: SequenceAnnotation
persistent identity: annotation2270789
display id: annotation2270789
version: 1
top level: BBa_I759009
location: https://synbiohub.org/public/igem/BBa_I759009/annotation2270789/range2270789/1
component: BBa_S03724

https://synbiohub.org/public/igem/BBa_I759009/annotation2270789/range2270789/1
type: Range
persistent identity: range2270789
display id: range2270789
version: 1
direction: direction/reverse
top level: BBa_I759009
start: 843
end: 1862
orientation: inline

BBa_I759008
type: SequenceAnnotation
persistent identity: annotation2270766
display id: annotation2270766
version: 1
top level: BBa_I759009
location: https://synbiohub.org/public/igem/BBa_I759009/annotation2270766/range2270766/1
component: BBa_I759008

https://synbiohub.org/public/igem/BBa_I759009/annotation2270766/range2270766/1
type: Range
persistent identity: range2270766
display id: range2270766
version: 1
direction: direction/reverse
top level: BBa_I759009
start: 1
end: 834
orientation: inline

dxs
type: ComponentDefinition
persistent identity: BBa K118000
display id: BBa_K118000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dxs coding sequence encoding 1-deoxyxylulose-5-phosphate synthase
creator: Chris French
created: 2008-07-08T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of dxs from Escherichia coli JM109. It encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses teh first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here].
mutable notes: No special considerations.
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dxs coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118000_sequence/1

dxs coding sequence
type: SequenceAnnotation
persistent identity: annotation1995699
display id: annotation1995699
version: 1
top level: dxs
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118000/annotation1995699/range1995699/1

https://synbiohub.org/public/igem/BBa_K118000/annotation1995699/range1995699/1
type: Range
persistent identity: range1995699
display id: range1995699
version: 1
direction: direction/forward
top level: dxs
start: 1
end: 1863
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0160
display id: BBa_C0160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme aiiA (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0060 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aiiA
sequence: https://synbiohub.org/public/igem/BBa_C0160_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1891585
display id: annotation1891585
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1

https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1
type: Range
persistent identity: range1891585
display id: range1891585
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 756
orientation: inline

BBa_F2622
type: ComponentDefinition
persistent identity: BBa F2622
display id: BBa_F2622
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Receiver Device
creator: Barry Canton
created: 2004-12-14T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the lac repressible R0011.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_R0062
component: BBa_C0062
component: BBa_R0011
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_F2622_sequence/1

BBa_B0012
type: Component
persistent identity: component1484681
display id: component1484681
version: 1
top level: BBa_F2622
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: Component
persistent identity: component1484696
display id: component1484696
version: 1
top level: BBa_F2622
access: public
definition: lux pR

BBa_C0062
type: Component
persistent identity: component1484663
display id: component1484663
version: 1
top level: BBa_F2622
access: public
definition: luxr

BBa_R0011
type: Component
persistent identity: component1484639
display id: component1484639
version: 1
top level: BBa_F2622
access: public
definition: lacI+pL

BBa_B0010
type: Component
persistent identity: component1484671
display id: component1484671
version: 1
top level: BBa_F2622
access: public
definition: BBa_B0010

BBa_B0034
type: Component
persistent identity: component1484648
display id: component1484648
version: 1
top level: BBa_F2622
access: public
definition: BBa_B0034

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1484696
display id: annotation1484696
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484696/range1484696/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_F2622/annotation1484696/range1484696/1
type: Range
persistent identity: range1484696
display id: range1484696
version: 1
direction: direction/forward
top level: BBa_F2622
start: 1008
end: 1062
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1484663
display id: annotation1484663
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484663/range1484663/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_F2622/annotation1484663/range1484663/1
type: Range
persistent identity: range1484663
display id: range1484663
version: 1
direction: direction/forward
top level: BBa_F2622
start: 82
end: 837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484648
display id: annotation1484648
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484648/range1484648/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2622/annotation1484648/range1484648/1
type: Range
persistent identity: range1484648
display id: range1484648
version: 1
direction: direction/forward
top level: BBa_F2622
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1484681
display id: annotation1484681
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484681/range1484681/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F2622/annotation1484681/range1484681/1
type: Range
persistent identity: range1484681
display id: range1484681
version: 1
direction: direction/forward
top level: BBa_F2622
start: 959
end: 999
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1484639
display id: annotation1484639
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484639/range1484639/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_F2622/annotation1484639/range1484639/1
type: Range
persistent identity: range1484639
display id: range1484639
version: 1
direction: direction/forward
top level: BBa_F2622
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484671
display id: annotation1484671
version: 1
top level: BBa_F2622
location: https://synbiohub.org/public/igem/BBa_F2622/annotation1484671/range1484671/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F2622/annotation1484671/range1484671/1
type: Range
persistent identity: range1484671
display id: range1484671
version: 1
direction: direction/forward
top level: BBa_F2622
start: 871
end: 950
orientation: inline

BBa_I759019
type: ComponentDefinition
persistent identity: BBa I759019
display id: BBa_I759019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cis7-repressed, tet-regulated YFP
creator: Kat Pak
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: true
experience: experience/None
group u list: _115_
m user id: 0
owner id: 1834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has been shown to repress the expression of YFP via a cis-repressive element called cr7 which sequesters the ribosome binding site of YFP.
mutable provenance: The cr element is a synthetic oligo which has been inserted into part I759009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S03724
component: BBa_I759044
sequence annotation: BBa_I759044
sequence annotation: BBa_S03724
sequence: https://synbiohub.org/public/igem/BBa_I759019_sequence/1

BBa_S03724
type: Component
persistent identity: component2271253
display id: component2271253
version: 1
top level: BBa_I759019
access: public
definition: https://synbiohub.org/public/igem/BBa_S03724/1

BBa_I759044
type: Component
persistent identity: component2271230
display id: component2271230
version: 1
top level: BBa_I759019
access: public
definition: https://synbiohub.org/public/igem/BBa_I759044/1

BBa_I759044
type: SequenceAnnotation
persistent identity: annotation2271230
display id: annotation2271230
version: 1
top level: BBa_I759019
location: https://synbiohub.org/public/igem/BBa_I759019/annotation2271230/range2271230/1
component: BBa_I759044

https://synbiohub.org/public/igem/BBa_I759019/annotation2271230/range2271230/1
type: Range
persistent identity: range2271230
display id: range2271230
version: 1
direction: direction/reverse
top level: BBa_I759019
start: 1
end: 833
orientation: inline

BBa_S03724
type: SequenceAnnotation
persistent identity: annotation2271253
display id: annotation2271253
version: 1
top level: BBa_I759019
location: https://synbiohub.org/public/igem/BBa_I759019/annotation2271253/range2271253/1
component: BBa_S03724

https://synbiohub.org/public/igem/BBa_I759019/annotation2271253/range2271253/1
type: Range
persistent identity: range2271253
display id: range2271253
version: 1
direction: direction/reverse
top level: BBa_I759019
start: 842
end: 1861
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
creator: jcbraff
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP (mut3b) [note that this part does not have a barcode]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
direction: direction/forward
top level: GFP
start: 1
end: 720
orientation: inline

BBa_I759005
type: ComponentDefinition
persistent identity: BBa I759005
display id: BBa_I759005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tet-regulated cro (Weak tetR promoter)
creator: Josh Michener
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _115_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pTet-spacer-cro-Term-J23116-tetR-Term
mutable notes: None
mutable provenance: Caltech iGEM
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I759002
component: BBa_S03724
sequence annotation: BBa_S03724
sequence annotation: BBa_I759002
sequence: https://synbiohub.org/public/igem/BBa_I759005_sequence/1

BBa_I759002
type: Component
persistent identity: component2270610
display id: component2270610
version: 1
top level: BBa_I759005
access: public
definition: https://synbiohub.org/public/igem/BBa_I759002/1

BBa_S03724
type: Component
persistent identity: component2270633
display id: component2270633
version: 1
top level: BBa_I759005
access: public
definition: https://synbiohub.org/public/igem/BBa_S03724/1

BBa_S03724
type: SequenceAnnotation
persistent identity: annotation2270633
display id: annotation2270633
version: 1
top level: BBa_I759005
location: https://synbiohub.org/public/igem/BBa_I759005/annotation2270633/range2270633/1
component: BBa_S03724

https://synbiohub.org/public/igem/BBa_I759005/annotation2270633/range2270633/1
type: Range
persistent identity: range2270633
display id: range2270633
version: 1
direction: direction/reverse
top level: BBa_I759005
start: 321
end: 1340
orientation: inline

BBa_I759002
type: SequenceAnnotation
persistent identity: annotation2270610
display id: annotation2270610
version: 1
top level: BBa_I759005
location: https://synbiohub.org/public/igem/BBa_I759005/annotation2270610/range2270610/1
component: BBa_I759002

https://synbiohub.org/public/igem/BBa_I759005/annotation2270610/range2270610/1
type: Range
persistent identity: range2270610
display id: range2270610
version: 1
direction: direction/reverse
top level: BBa_I759005
start: 1
end: 312
orientation: inline

BBa_K1149051
type: ComponentDefinition
persistent identity: BBa K1149051
display id: BBa_K1149051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Hybrid promoter phaCAB
creator: Margarita Kopniczky
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1461_
m user id: 0
owner id: 11706
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: J23104+RBS B0034-phaCAB
mutable notes: added different promoter RBS to increase expression
mutable provenance: comes from exisiting biobrick parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_K934001
component: BBa_J23104
sequence annotation: BBa_B0034
sequence annotation: BBa_J23104
sequence annotation: BBa_K934001
sequence: https://synbiohub.org/public/igem/BBa_K1149051_sequence/1

BBa_B0034
type: Component
persistent identity: component2363312
display id: component2363312
version: 1
top level: BBa_K1149051
access: public
definition: BBa_B0034

BBa_K934001
type: Component
persistent identity: component2363320
display id: component2363320
version: 1
top level: BBa_K1149051
access: public
definition: https://synbiohub.org/public/igem/BBa_K934001/1

BBa_J23104
type: Component
persistent identity: component2363310
display id: component2363310
version: 1
top level: BBa_K1149051
access: public
definition: BBa_J23104

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2363312
display id: annotation2363312
version: 1
top level: BBa_K1149051
location: https://synbiohub.org/public/igem/BBa_K1149051/annotation2363312/range2363312/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1149051/annotation2363312/range2363312/1
type: Range
persistent identity: range2363312
display id: range2363312
version: 1
direction: direction/reverse
top level: BBa_K1149051
start: 44
end: 55
orientation: inline

BBa_J23104
type: SequenceAnnotation
persistent identity: annotation2363310
display id: annotation2363310
version: 1
top level: BBa_K1149051
location: https://synbiohub.org/public/igem/BBa_K1149051/annotation2363310/range2363310/1
component: BBa_J23104

https://synbiohub.org/public/igem/BBa_K1149051/annotation2363310/range2363310/1
type: Range
persistent identity: range2363310
display id: range2363310
version: 1
direction: direction/reverse
top level: BBa_K1149051
start: 1
end: 35
orientation: inline

BBa_K934001
type: SequenceAnnotation
persistent identity: annotation2363320
display id: annotation2363320
version: 1
top level: BBa_K1149051
location: https://synbiohub.org/public/igem/BBa_K1149051/annotation2363320/range2363320/1
component: BBa_K934001

https://synbiohub.org/public/igem/BBa_K1149051/annotation2363320/range2363320/1
type: Range
persistent identity: range2363320
display id: range2363320
version: 1
direction: direction/reverse
top level: BBa_K1149051
start: 64
end: 4271
orientation: inline

BBa_K1033916
type: ComponentDefinition
persistent identity: BBa K1033916
display id: BBa_K1033916
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: amajLime, yellow-green chromoprotein
creator: Sabri Jamal
created: 2013-08-24T11:00:00Z
modified: 2015-05-08T01:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 0
owner id: 10137
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein from the coral Anemonia majano, amajLime, naturally exhibits strong color when expressed.
mutable notes: Synthesized and codon optimized for E coli by GenScript.
mutable provenance: The anemone Anemonia majano.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: amajLime
sequence: https://synbiohub.org/public/igem/BBa_K1033916_sequence/1

amajLime
type: SequenceAnnotation
persistent identity: annotation2332103
display id: annotation2332103
version: 1
top level: BBa_K1033916
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033916/annotation2332103/range2332103/1

https://synbiohub.org/public/igem/BBa_K1033916/annotation2332103/range2332103/1
type: Range
persistent identity: range2332103
display id: range2332103
version: 1
direction: direction/forward
top level: BBa_K1033916
start: 1
end: 690
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: engineered cyan fluorescent protein derived from A. victoria GFP
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (lacI regulated, lambda pL hybrid)
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
direction: direction/forward
top level: lacI+pL
start: 26
end: 42
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
direction: direction/forward
top level: lacI+pL
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
direction: direction/forward
top level: lacI+pL
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
direction: direction/forward
top level: lacI+pL
start: 43
end: 48
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
direction: direction/forward
top level: lacI+pL
start: 1
end: 54
orientation: inline

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c2 repressor from Salmonella phage P22 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: BBa_C0053
sequence annotation: cII p22
sequence annotation: Help:Barcodes
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
direction: direction/forward
top level: c2 P22
start: 649
end: 681
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 687
orientation: inline

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
direction: direction/forward
top level: c2 P22
start: 1
end: 648
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
direction: direction/forward
top level: c2 P22
start: 688
end: 712
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
direction: direction/forward
top level: c2 P22
start: 682
end: 687
orientation: inline

BBa_K145279
type: ComponentDefinition
persistent identity: BBa K145279
display id: BBa_K145279
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP regulated by tetracyclin
creator: Nathalie Busschaert
created: 2008-09-06T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: this device consists if a TetR generator and a GFP under the control of a TetR regulated promoter. Normally the production of GFP is switched off. It can be switched on by the addition of tetracyclin (or anhydrotetracyclin).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0240
component: BBa_K145201
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_E0240
sequence annotation: BBa_K145201
sequence: https://synbiohub.org/public/igem/BBa_K145279_sequence/1

BBa_E0240
type: Component
persistent identity: component2282138
display id: component2282138
version: 1
top level: BBa_K145279
access: public
definition: GFP report

BBa_K145201
type: Component
persistent identity: component2282122
display id: component2282122
version: 1
top level: BBa_K145279
access: public
definition: https://synbiohub.org/public/igem/BBa_K145201/1

BBa_R0040
type: Component
persistent identity: component2282123
display id: component2282123
version: 1
top level: BBa_K145279
access: public
definition: p(tetR)

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2282123
display id: annotation2282123
version: 1
top level: BBa_K145279
location: https://synbiohub.org/public/igem/BBa_K145279/annotation2282123/range2282123/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K145279/annotation2282123/range2282123/1
type: Range
persistent identity: range2282123
display id: range2282123
version: 1
direction: direction/forward
top level: BBa_K145279
start: 892
end: 945
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2282138
display id: annotation2282138
version: 1
top level: BBa_K145279
location: https://synbiohub.org/public/igem/BBa_K145279/annotation2282138/range2282138/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K145279/annotation2282138/range2282138/1
type: Range
persistent identity: range2282138
display id: range2282138
version: 1
direction: direction/reverse
top level: BBa_K145279
start: 954
end: 1829
orientation: inline

BBa_K145201
type: SequenceAnnotation
persistent identity: annotation2282122
display id: annotation2282122
version: 1
top level: BBa_K145279
location: https://synbiohub.org/public/igem/BBa_K145279/annotation2282122/range2282122/1
component: BBa_K145201

https://synbiohub.org/public/igem/BBa_K145279/annotation2282122/range2282122/1
type: Range
persistent identity: range2282122
display id: range2282122
version: 1
direction: direction/reverse
top level: BBa_K145279
start: 1
end: 883
orientation: inline

BBa_J52014
type: ComponentDefinition
persistent identity: BBa J52014
display id: BBa_J52014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NFkB-dnMyD88-link-rLuc
creator: Rok Tkavc
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 853
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is a composite part, coposed of NF-kB dependent promotor and dominant negative human MyD88
mutable notes: later
mutable provenance: later
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J52010
component: BBa_J52011
sequence annotation: BBa_J52011
sequence annotation: BBa_J52010
sequence: https://synbiohub.org/public/igem/BBa_J52014_sequence/1

BBa_J52010
type: Component
persistent identity: component1903112
display id: component1903112
version: 1
top level: BBa_J52014
access: public
definition: https://synbiohub.org/public/igem/BBa_J52010/1

BBa_J52011
type: Component
persistent identity: component1903117
display id: component1903117
version: 1
top level: BBa_J52014
access: public
definition: BBa_J52011

BBa_J52011
type: SequenceAnnotation
persistent identity: annotation1903117
display id: annotation1903117
version: 1
top level: BBa_J52014
location: https://synbiohub.org/public/igem/BBa_J52014/annotation1903117/range1903117/1
component: BBa_J52011

https://synbiohub.org/public/igem/BBa_J52014/annotation1903117/range1903117/1
type: Range
persistent identity: range1903117
display id: range1903117
version: 1
direction: direction/reverse
top level: BBa_J52014
start: 821
end: 2191
orientation: inline

BBa_J52010
type: SequenceAnnotation
persistent identity: annotation1903112
display id: annotation1903112
version: 1
top level: BBa_J52014
location: https://synbiohub.org/public/igem/BBa_J52014/annotation1903112/range1903112/1
component: BBa_J52010

https://synbiohub.org/public/igem/BBa_J52014/annotation1903112/range1903112/1
type: Range
persistent identity: range1903112
display id: range1903112
version: 1
direction: direction/reverse
top level: BBa_J52014
start: 1
end: 814
orientation: inline

BBa_S03154
type: ComponentDefinition
persistent identity: BBa S03154
display id: BBa_S03154
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0078
creator: Jennifer Braff
created: 2004-07-22T11:00:00Z
modified: 2015-05-08T01:14:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0078
component: BBa_B0034
sequence annotation: BBa_C0078
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03154_sequence/1

BBa_C0078
type: Component
persistent identity: component940972
display id: component940972
version: 1
top level: BBa_S03154
access: public
definition: https://synbiohub.org/public/igem/BBa_C0078/1

BBa_B0034
type: Component
persistent identity: component940962
display id: component940962
version: 1
top level: BBa_S03154
access: public
definition: BBa_B0034

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation940972
display id: annotation940972
version: 1
top level: BBa_S03154
location: https://synbiohub.org/public/igem/BBa_S03154/annotation940972/range940972/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_S03154/annotation940972/range940972/1
type: Range
persistent identity: range940972
display id: range940972
version: 1
direction: direction/forward
top level: BBa_S03154
start: 19
end: 660
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation940962
display id: annotation940962
version: 1
top level: BBa_S03154
location: https://synbiohub.org/public/igem/BBa_S03154/annotation940962/range940962/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03154/annotation940962/range940962/1
type: Range
persistent identity: range940962
display id: range940962
version: 1
direction: direction/forward
top level: BBa_S03154
start: 1
end: 12
orientation: inline

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (LasR & PAI regulated)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Binding region for LasR protein (positive regulation)
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OP2
sequence annotation: -10
sequence annotation: OP1
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
direction: direction/forward
top level: LasR+PAI
start: 46
end: 63
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
direction: direction/forward
top level: LasR+PAI
start: 140
end: 145
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
direction: direction/forward
top level: LasR+PAI
start: 106
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
direction: direction/forward
top level: LasR+PAI
start: 117
end: 122
orientation: inline

PchA/PchB
type: ComponentDefinition
persistent identity: BBa J45319
display id: BBa_J45319
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PchA & PchB enzyme generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is an intermediate to the protein generator BBa_45300. This part is almost identical to BBa_J45299 except that is uses as its RBS B0032 instead of B0030.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_J45017
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_J45017
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45319_sequence/1

BBa_B0032
type: Component
persistent identity: component1960931
display id: component1960931
version: 1
top level: PchA/PchB
access: public
definition: BBa_B0032

BBa_J45017
type: Component
persistent identity: component1960938
display id: component1960938
version: 1
top level: PchA/PchB
access: public
definition: https://synbiohub.org/public/igem/BBa_J45017/1

BBa_B0010
type: Component
persistent identity: component1960939
display id: component1960939
version: 1
top level: PchA/PchB
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component1960941
display id: component1960941
version: 1
top level: PchA/PchB
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1960931
display id: annotation1960931
version: 1
top level: PchA/PchB
location: https://synbiohub.org/public/igem/BBa_J45319/annotation1960931/range1960931/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45319/annotation1960931/range1960931/1
type: Range
persistent identity: range1960931
display id: range1960931
version: 1
direction: direction/forward
top level: PchA/PchB
start: 1
end: 13
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960939
display id: annotation1960939
version: 1
top level: PchA/PchB
location: https://synbiohub.org/public/igem/BBa_J45319/annotation1960939/range1960939/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45319/annotation1960939/range1960939/1
type: Range
persistent identity: range1960939
display id: range1960939
version: 1
direction: direction/forward
top level: PchA/PchB
start: 1764
end: 1843
orientation: inline

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation1960938
display id: annotation1960938
version: 1
top level: PchA/PchB
location: https://synbiohub.org/public/igem/BBa_J45319/annotation1960938/range1960938/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_J45319/annotation1960938/range1960938/1
type: Range
persistent identity: range1960938
display id: range1960938
version: 1
direction: direction/reverse
top level: PchA/PchB
start: 20
end: 1755
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960941
display id: annotation1960941
version: 1
top level: PchA/PchB
location: https://synbiohub.org/public/igem/BBa_J45319/annotation1960941/range1960941/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45319/annotation1960941/range1960941/1
type: Range
persistent identity: range1960941
display id: range1960941
version: 1
direction: direction/forward
top level: PchA/PchB
start: 1852
end: 1892
orientation: inline

BBa_I6057
type: ComponentDefinition
persistent identity: BBa I6057
display id: BBa_I6057
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test I0500.E0420
creator: cconboy
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0020
component: BBa_I0500
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E0020
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I6057_sequence/1

BBa_E0020
type: Component
persistent identity: component948835
display id: component948835
version: 1
top level: BBa_I6057
access: public
definition: ecfp

BBa_I0500
type: Component
persistent identity: component948828
display id: component948828
version: 1
top level: BBa_I6057
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component948833
display id: component948833
version: 1
top level: BBa_I6057
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component948840
display id: component948840
version: 1
top level: BBa_I6057
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component948850
display id: component948850
version: 1
top level: BBa_I6057
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948850
display id: annotation948850
version: 1
top level: BBa_I6057
location: https://synbiohub.org/public/igem/BBa_I6057/annotation948850/range948850/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6057/annotation948850/range948850/1
type: Range
persistent identity: range948850
display id: range948850
version: 1
direction: direction/forward
top level: BBa_I6057
start: 2056
end: 2096
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948833
display id: annotation948833
version: 1
top level: BBa_I6057
location: https://synbiohub.org/public/igem/BBa_I6057/annotation948833/range948833/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6057/annotation948833/range948833/1
type: Range
persistent identity: range948833
display id: range948833
version: 1
direction: direction/forward
top level: BBa_I6057
start: 1219
end: 1230
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948840
display id: annotation948840
version: 1
top level: BBa_I6057
location: https://synbiohub.org/public/igem/BBa_I6057/annotation948840/range948840/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6057/annotation948840/range948840/1
type: Range
persistent identity: range948840
display id: range948840
version: 1
direction: direction/forward
top level: BBa_I6057
start: 1968
end: 2047
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation948835
display id: annotation948835
version: 1
top level: BBa_I6057
location: https://synbiohub.org/public/igem/BBa_I6057/annotation948835/range948835/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I6057/annotation948835/range948835/1
type: Range
persistent identity: range948835
display id: range948835
version: 1
direction: direction/forward
top level: BBa_I6057
start: 1237
end: 1959
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation948828
display id: annotation948828
version: 1
top level: BBa_I6057
location: https://synbiohub.org/public/igem/BBa_I6057/annotation948828/range948828/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I6057/annotation948828/range948828/1
type: Range
persistent identity: range948828
display id: range948828
version: 1
direction: direction/forward
top level: BBa_I6057
start: 1
end: 1210
orientation: inline

BBa_K1150002
type: ComponentDefinition
persistent identity: BBa K1150002
display id: BBa_K1150002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KRAB
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: KRAB is ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: KRAB
sequence: https://synbiohub.org/public/igem/BBa_K1150002_sequence/1

KRAB
type: SequenceAnnotation
persistent identity: annotation2347616
display id: annotation2347616
version: 1
top level: BBa_K1150002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1150002/annotation2347616/range2347616/1

https://synbiohub.org/public/igem/BBa_K1150002/annotation2347616/range2347616/1
type: Range
persistent identity: range2347616
display id: range2347616
version: 1
direction: direction/forward
top level: BBa_K1150002
start: 1
end: 366
orientation: inline

BBa_K1150036
type: ComponentDefinition
persistent identity: BBa K1150036
display id: BBa_K1150036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: uniCAS RNAimer to target VEGF1
creator: Ilona Blank
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ...
mutable notes: ..
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1150036_sequence/1

BBa_I746107
type: ComponentDefinition
persistent identity: BBa I746107
display id: BBa_I746107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I746101 (AIP sensor infrastructure) + I746105 (Inducible P2 + GFP reporter)
creator: Lovelace Soirez
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2127
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is the AIP sensor infrastructure ([http://partsregistry.org/wiki/index.php/Part:BBa_I746101 I746101]) from the agr system with an AIP-inducible promoter P2 and GFP reporter ([http://partsregistry.org/wiki/index.php/Part:BBa_I746105 I746105]) downstream. A constitutive/inducible promoter may be added upstream of this part, which will cause production of the components of the signal transduction pathway (AgrC and AgrA); when phosphorylated AgrA is present, the AIP-sensitive promoter (P2) is activated and GFP ([http://partsregistry.org/wiki/index.php/Part:BBa_E0840 E0840]) is output. The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the activity of agr P2 promoter about 50-fold.
mutable notes: N/A
mutable provenance: The original sequences for agrC and agrA were taken from the S. aureus Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_B0030
component: BBa_I746100
component: BBa_I746104
component: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_I746100
sequence annotation: BBa_I746104
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I746107_sequence/1

BBa_B0012
type: Component
persistent identity: component1952715
display id: component1952715
version: 1
top level: BBa_I746107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1952713
display id: component1952713
version: 1
top level: BBa_I746107
access: public
definition: BBa_B0010

BBa_B0030
type: Component
persistent identity: component1952709
display id: component1952709
version: 1
top level: BBa_I746107
access: public
definition: BBa_B0030

BBa_B0010
type: Component
persistent identity: component1952701
display id: component1952701
version: 1
top level: BBa_I746107
access: public
definition: BBa_B0010

BBa_I746100
type: Component
persistent identity: component1952700
display id: component1952700
version: 1
top level: BBa_I746107
access: public
definition: https://synbiohub.org/public/igem/BBa_I746100/1

BBa_I746104
type: Component
persistent identity: component1952707
display id: component1952707
version: 1
top level: BBa_I746107
access: public
definition: https://synbiohub.org/public/igem/BBa_I746104/1

BBa_B0012
type: Component
persistent identity: component1952703
display id: component1952703
version: 1
top level: BBa_I746107
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1952712
display id: component1952712
version: 1
top level: BBa_I746107
access: public
definition: GFP

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1952701
display id: annotation1952701
version: 1
top level: BBa_I746107
location: https://synbiohub.org/public/igem/BBa_I746107/annotation1952701/range1952701/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746107/annotation1952701/range1952701/1
type: Range
persistent identity: range1952701
display id: range1952701
version: 1
direction: direction/forward
top level: BBa_I746107
start: 1929
end: 2008
orientation: inline

BBa_I746100
type: SequenceAnnotation
persistent identity: annotation1952700
display id: annotation1952700
version: 1
top level: BBa_I746107
location: https://synbiohub.org/public/igem/BBa_I746107/annotation1952700/range1952700/1
component: BBa_I746100

https://synbiohub.org/public/igem/BBa_I746107/annotation1952700/range1952700/1
type: Range
persistent identity: range1952700
display id: range1952700
version: 1
direction: direction/reverse
top level: BBa_I746107
start: 1
end: 1920
orientation: inline

BBa_I746104
type: SequenceAnnotation
persistent identity: annotation1952707
display id: annotation1952707
version: 1
top level: BBa_I746107
location: https://synbiohub.org/public/igem/BBa_I746107/annotation1952707/range1952707/1
component: BBa_I746104

https://synbiohub.org/public/igem/BBa_I746107/annotation1952707/range1952707/1
type: Range
persistent identity: range1952707
display id: range1952707
version: 1
direction: direction/reverse
top level: BBa_I746107
start: 2066
end: 2161
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1952715
display id: annotation1952715
version: 1
top level: BBa_I746107
location: https://synbiohub.org/public/igem/BBa_I746107/annotation1952715/range1952715/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746107/annotation1952715/range1952715/1
type: Range
persistent identity: range1952715
display id: range1952715
version: 1
direction: direction/forward
top level: BBa_I746107
start: 3007
end: 3047
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1952709
display id: annotation1952709
version: 1
top level: BBa_I746107
location: https://synbiohub.org/public/igem/BBa_I746107/annotation1952709/range1952709/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I746107/annotation1952709/range1952709/1
type: Range
persistent identity: range1952709
display id: range1952709
version: 1
direction: direction/forward
top level: BBa_I746107
start: 2170
end: 2184
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1952712
display id: annotation1952712
version: 1
top level: BBa_I746107
location: https://synbiohub.org/public/igem/BBa_I746107/annotation1952712/range1952712/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I746107/annotation1952712/range1952712/1
type: Range
persistent identity: range1952712
display id: range1952712
version: 1
direction: direction/reverse
top level: BBa_I746107
start: 2191
end: 2910
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1952713
display id: annotation1952713
version: 1
top level: BBa_I746107
location: https://synbiohub.org/public/igem/BBa_I746107/annotation1952713/range1952713/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746107/annotation1952713/range1952713/1
type: Range
persistent identity: range1952713
display id: range1952713
version: 1
direction: direction/forward
top level: BBa_I746107
start: 2919
end: 2998
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1952703
display id: annotation1952703
version: 1
top level: BBa_I746107
location: https://synbiohub.org/public/igem/BBa_I746107/annotation1952703/range1952703/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746107/annotation1952703/range1952703/1
type: Range
persistent identity: range1952703
display id: range1952703
version: 1
direction: direction/forward
top level: BBa_I746107
start: 2017
end: 2057
orientation: inline

BBa_J23117
type: ComponentDefinition
persistent identity: BBa J23117
display id: BBa_J23117
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23117_sequence/1

BBa_J52026
type: ComponentDefinition
persistent identity: BBa J52026
display id: BBa_J52026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-GFP
creator: Jelka Pohar
created: 2006-10-14T11:00:00Z
modified: 2016-01-26T09:39:38Z
discontinued: false
dominant: false
group u list: _80_
m user id: 4206
owner id: 855
owning group id: 80
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor). dnMyD88 is linked to GFP (acts as a reporter) through six aminoacid long protein linker.
mutable notes: dnMyD88-linker-GFP was made by using PCR overlap extension technique. This part is cloned in BioBrick vector pSB1AK3. Original sequence has PstI restriction site so we have to introduce a mutation without affect on amino acid sequence.
mutable provenance: dnMyD88- GFP- more to come
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88-linker-GFP
sequence annotation: GFP
sequence annotation: dnMyD88
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_J52026_sequence/1

dnMyD88-linker-GFP
type: SequenceAnnotation
persistent identity: annotation1903011
display id: annotation1903011
version: 1
top level: BBa_J52026
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903011/range1903011/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903011/range1903011/1
type: Range
persistent identity: range1903011
display id: range1903011
version: 1
direction: direction/forward
top level: BBa_J52026
start: 1
end: 1155
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation1903010
display id: annotation1903010
version: 1
top level: BBa_J52026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903010/range1903010/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903010/range1903010/1
type: Range
persistent identity: range1903010
display id: range1903010
version: 1
direction: direction/forward
top level: BBa_J52026
start: 436
end: 1155
orientation: inline

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1903008
display id: annotation1903008
version: 1
top level: BBa_J52026
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903008/range1903008/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903008/range1903008/1
type: Range
persistent identity: range1903008
display id: range1903008
version: 1
direction: direction/forward
top level: BBa_J52026
start: 1
end: 417
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903009
display id: annotation1903009
version: 1
top level: BBa_J52026
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52026/annotation1903009/range1903009/1

https://synbiohub.org/public/igem/BBa_J52026/annotation1903009/range1903009/1
type: Range
persistent identity: range1903009
display id: range1903009
version: 1
direction: direction/forward
top level: BBa_J52026
start: 418
end: 435
orientation: inline

BBa_J23114
type: ComponentDefinition
persistent identity: BBa J23114
display id: BBa_J23114
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23114_sequence/1

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from E. coli phage lambda (+LVA)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: cI lambda
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
direction: direction/forward
top level: cI lam
start: 751
end: 775
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
direction: direction/forward
top level: cI lam
start: 712
end: 744
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
direction: direction/forward
top level: cI lam
start: 4
end: 711
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.2 (weak) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
direction: direction/forward
top level: BBa_B0031
start: 7
end: 10
orientation: inline

BBa_I20260
type: ComponentDefinition
persistent identity: BBa I20260
display id: BBa_I20260
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Measurement Kit Test of J23101
creator: Jason Kelly
created: 2008-01-08T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: true
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Measkit test of J23101
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_E0040
component: BBa_B0032
component: BBa_B0010
component: BBa_B0012
component: BBa_J23101
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_J23101
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I20260_sequence/1

BBa_E0040
type: Component
persistent identity: component1959297
display id: component1959297
version: 1
top level: BBa_I20260
access: public
definition: GFP

BBa_B0032
type: Component
persistent identity: component1959294
display id: component1959294
version: 1
top level: BBa_I20260
access: public
definition: BBa_B0032

BBa_B0010
type: Component
persistent identity: component1959298
display id: component1959298
version: 1
top level: BBa_I20260
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component1959300
display id: component1959300
version: 1
top level: BBa_I20260
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J23101
type: Component
persistent identity: component1959292
display id: component1959292
version: 1
top level: BBa_I20260
access: public
definition: BBa_J23101

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1959297
display id: annotation1959297
version: 1
top level: BBa_I20260
location: https://synbiohub.org/public/igem/BBa_I20260/annotation1959297/range1959297/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I20260/annotation1959297/range1959297/1
type: Range
persistent identity: range1959297
display id: range1959297
version: 1
direction: direction/reverse
top level: BBa_I20260
start: 63
end: 782
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1959300
display id: annotation1959300
version: 1
top level: BBa_I20260
location: https://synbiohub.org/public/igem/BBa_I20260/annotation1959300/range1959300/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I20260/annotation1959300/range1959300/1
type: Range
persistent identity: range1959300
display id: range1959300
version: 1
direction: direction/forward
top level: BBa_I20260
start: 879
end: 919
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1959298
display id: annotation1959298
version: 1
top level: BBa_I20260
location: https://synbiohub.org/public/igem/BBa_I20260/annotation1959298/range1959298/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I20260/annotation1959298/range1959298/1
type: Range
persistent identity: range1959298
display id: range1959298
version: 1
direction: direction/forward
top level: BBa_I20260
start: 791
end: 870
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation1959292
display id: annotation1959292
version: 1
top level: BBa_I20260
location: https://synbiohub.org/public/igem/BBa_I20260/annotation1959292/range1959292/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_I20260/annotation1959292/range1959292/1
type: Range
persistent identity: range1959292
display id: range1959292
version: 1
direction: direction/reverse
top level: BBa_I20260
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1959294
display id: annotation1959294
version: 1
top level: BBa_I20260
location: https://synbiohub.org/public/igem/BBa_I20260/annotation1959294/range1959294/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I20260/annotation1959294/range1959294/1
type: Range
persistent identity: range1959294
display id: range1959294
version: 1
direction: direction/forward
top level: BBa_I20260
start: 44
end: 56
orientation: inline

BBa_I732920
type: ComponentDefinition
persistent identity: BBa I732920
display id: BBa_I732920
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015 J09855 I732835
creator: Zhan Jian
created: 2007-10-17T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0015
component: BBa_J09855
component: BBa_I732835
sequence annotation: BBa_J09855
sequence annotation: BBa_B0015
sequence annotation: BBa_I732835
sequence: https://synbiohub.org/public/igem/BBa_I732920_sequence/1

BBa_B0015
type: Component
persistent identity: component2296274
display id: component2296274
version: 1
top level: BBa_I732920
access: public
definition: https://synbiohub.org/public/igem/BBa_B0015/1

BBa_J09855
type: Component
persistent identity: component2296299
display id: component2296299
version: 1
top level: BBa_I732920
access: public
definition: https://synbiohub.org/public/igem/BBa_J09855/1

BBa_I732835
type: Component
persistent identity: component2296309
display id: component2296309
version: 1
top level: BBa_I732920
access: public
definition: https://synbiohub.org/public/igem/BBa_I732835/1

BBa_J09855
type: SequenceAnnotation
persistent identity: annotation2296299
display id: annotation2296299
version: 1
top level: BBa_I732920
location: https://synbiohub.org/public/igem/BBa_I732920/annotation2296299/range2296299/1
component: BBa_J09855

https://synbiohub.org/public/igem/BBa_I732920/annotation2296299/range2296299/1
type: Range
persistent identity: range2296299
display id: range2296299
version: 1
direction: direction/reverse
top level: BBa_I732920
start: 138
end: 1181
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2296274
display id: annotation2296274
version: 1
top level: BBa_I732920
location: https://synbiohub.org/public/igem/BBa_I732920/annotation2296274/range2296274/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I732920/annotation2296274/range2296274/1
type: Range
persistent identity: range2296274
display id: range2296274
version: 1
direction: direction/reverse
top level: BBa_I732920
start: 1
end: 129
orientation: inline

BBa_I732835
type: SequenceAnnotation
persistent identity: annotation2296309
display id: annotation2296309
version: 1
top level: BBa_I732920
location: https://synbiohub.org/public/igem/BBa_I732920/annotation2296309/range2296309/1
component: BBa_I732835

https://synbiohub.org/public/igem/BBa_I732920/annotation2296309/range2296309/1
type: Range
persistent identity: range2296309
display id: range2296309
version: 1
direction: direction/reverse
top level: BBa_I732920
start: 1190
end: 2430
orientation: inline

BBa_K115003
type: ComponentDefinition
persistent identity: BBa K115003
display id: BBa_K115003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA thermometer (PrfA)
creator: O.M.J.A. Stassen
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3007
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is designed as a temperature inducible RBS. Only designed so far.
mutable notes: The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
mutable provenance: This sequence is taken from the Listeria Monocytogenes (EU372032.). It's the 5'UTR of a TF induced at 37 degrees.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: SD
sequence annotation: Predicted stem loop up to A of AUG
sequence: https://synbiohub.org/public/igem/BBa_K115003_sequence/1

SD
type: SequenceAnnotation
persistent identity: annotation1966983
display id: annotation1966983
version: 1
top level: BBa_K115003
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K115003/annotation1966983/range1966983/1

https://synbiohub.org/public/igem/BBa_K115003/annotation1966983/range1966983/1
type: Range
persistent identity: range1966983
display id: range1966983
version: 1
direction: direction/forward
top level: BBa_K115003
start: 106
end: 109
orientation: inline

Predicted stem loop up to A of AUG
type: SequenceAnnotation
persistent identity: annotation1967003
display id: annotation1967003
version: 1
top level: BBa_K115003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K115003/annotation1967003/range1967003/1

https://synbiohub.org/public/igem/BBa_K115003/annotation1967003/range1967003/1
type: Range
persistent identity: range1967003
display id: range1967003
version: 1
direction: direction/forward
top level: BBa_K115003
start: 28
end: 109
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS (Elowitz 1999) -- defines RBS efficiency
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS based on Elowitz repressilator.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
direction: direction/forward
top level: BBa_B0034
start: 5
end: 8
orientation: inline

BBa_I759027
type: ComponentDefinition
persistent identity: BBa I759027
display id: BBa_I759027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cis3-repressed, tet-regulated Q
creator: Josh Michener
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _115_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Robbie
mutable notes: Robbie
mutable provenance: Robbie
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_S03724
component: BBa_I759011
sequence annotation: BBa_I759011
sequence annotation: BBa_S03724
sequence: https://synbiohub.org/public/igem/BBa_I759027_sequence/1

BBa_S03724
type: Component
persistent identity: component2271381
display id: component2271381
version: 1
top level: BBa_I759027
access: public
definition: https://synbiohub.org/public/igem/BBa_S03724/1

BBa_I759011
type: Component
persistent identity: component2271358
display id: component2271358
version: 1
top level: BBa_I759027
access: public
definition: https://synbiohub.org/public/igem/BBa_I759011/1

BBa_I759011
type: SequenceAnnotation
persistent identity: annotation2271358
display id: annotation2271358
version: 1
top level: BBa_I759027
location: https://synbiohub.org/public/igem/BBa_I759027/annotation2271358/range2271358/1
component: BBa_I759011

https://synbiohub.org/public/igem/BBa_I759027/annotation2271358/range2271358/1
type: Range
persistent identity: range2271358
display id: range2271358
version: 1
direction: direction/reverse
top level: BBa_I759027
start: 1
end: 738
orientation: inline

BBa_S03724
type: SequenceAnnotation
persistent identity: annotation2271381
display id: annotation2271381
version: 1
top level: BBa_I759027
location: https://synbiohub.org/public/igem/BBa_I759027/annotation2271381/range2271381/1
component: BBa_S03724

https://synbiohub.org/public/igem/BBa_I759027/annotation2271381/range2271381/1
type: Range
persistent identity: range2271381
display id: range2271381
version: 1
direction: direction/reverse
top level: BBa_I759027
start: 747
end: 1766
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (+LVA)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI 434
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: BBa_C0052
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
direction: direction/forward
top level: cI 434
start: 670
end: 694
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
direction: direction/forward
top level: cI 434
start: 631
end: 669
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 669
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (luxR & HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0062
sequence annotation: -35
sequence annotation: start
sequence annotation: -10
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
direction: direction/forward
top level: lux pR
start: 53
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 47
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

BBa_K1150001
type: ComponentDefinition
persistent identity: BBa K1150001
display id: BBa_K1150001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: VP16
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: VP16 is ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: VP16
sequence: https://synbiohub.org/public/igem/BBa_K1150001_sequence/1

VP16
type: SequenceAnnotation
persistent identity: annotation2347617
display id: annotation2347617
version: 1
top level: BBa_K1150001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1150001/annotation2347617/range2347617/1

https://synbiohub.org/public/igem/BBa_K1150001/annotation2347617/range2347617/1
type: Range
persistent identity: range2347617
display id: range2347617
version: 1
direction: direction/forward
top level: BBa_K1150001
start: 1
end: 372
orientation: inline

BBa_E0420
type: ComponentDefinition
persistent identity: BBa E0420
display id: BBa_E0420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
creator: Caitlin Conboy
created: 2004-03-15T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Standard CFP output device w/o LVA tag.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_E0020
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_E0420_sequence/1

BBa_B0012
type: Component
persistent identity: component942797
display id: component942797
version: 1
top level: BBa_E0420
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component942782
display id: component942782
version: 1
top level: BBa_E0420
access: public
definition: ecfp

BBa_B0034
type: Component
persistent identity: component942780
display id: component942780
version: 1
top level: BBa_E0420
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component942787
display id: component942787
version: 1
top level: BBa_E0420
access: public
definition: BBa_B0010

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942787
display id: annotation942787
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942787/range942787/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0420/annotation942787/range942787/1
type: Range
persistent identity: range942787
display id: range942787
version: 1
direction: direction/forward
top level: BBa_E0420
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942797
display id: annotation942797
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942797/range942797/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0420/annotation942797/range942797/1
type: Range
persistent identity: range942797
display id: range942797
version: 1
direction: direction/forward
top level: BBa_E0420
start: 838
end: 878
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation942782
display id: annotation942782
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942782/range942782/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_E0420/annotation942782/range942782/1
type: Range
persistent identity: range942782
display id: range942782
version: 1
direction: direction/forward
top level: BBa_E0420
start: 19
end: 741
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942780
display id: annotation942780
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942780/range942780/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0420/annotation942780/range942780/1
type: Range
persistent identity: range942780
display id: range942780
version: 1
direction: direction/forward
top level: BBa_E0420
start: 1
end: 12
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL-making Enzyme, luxI (+RBS)
creator: robertb
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0034.C0061
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
access: public
definition: luxI

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
type: Range
persistent identity: range1060403
display id: range1060403
version: 1
direction: direction/forward
top level: BBa_C0261
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
direction: direction/forward
top level: BBa_C0261
start: 19
end: 636
orientation: inline

BBa_J23104
type: ComponentDefinition
persistent identity: BBa J23104
display id: BBa_J23104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23104_sequence/1

BBa_I0466
type: ComponentDefinition
persistent identity: BBa I0466
display id: BBa_I0466
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR Protein Generator
creator: mit
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR can sense N-butyryl-HSL in the media and can activate transcription from R0071. It can be used as part of a receiver device.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_C0071
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_C0071
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I0466_sequence/1

BBa_B0034
type: Component
persistent identity: component943304
display id: component943304
version: 1
top level: BBa_I0466
access: public
definition: BBa_B0034

BBa_C0071
type: Component
persistent identity: component943314
display id: component943314
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_C0071/1

BBa_B0012
type: Component
persistent identity: component943330
display id: component943330
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component943320
display id: component943320
version: 1
top level: BBa_I0466
access: public
definition: BBa_B0010

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation943314
display id: annotation943314
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943314/range943314/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_I0466/annotation943314/range943314/1
type: Range
persistent identity: range943314
display id: range943314
version: 1
direction: direction/forward
top level: BBa_I0466
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943320
display id: annotation943320
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943320/range943320/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0466/annotation943320/range943320/1
type: Range
persistent identity: range943320
display id: range943320
version: 1
direction: direction/forward
top level: BBa_I0466
start: 814
end: 893
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943330
display id: annotation943330
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943330/range943330/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0466/annotation943330/range943330/1
type: Range
persistent identity: range943330
display id: range943330
version: 1
direction: direction/forward
top level: BBa_I0466
start: 902
end: 942
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943304
display id: annotation943304
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943304/range943304/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0466/annotation943304/range943304/1
type: Range
persistent identity: range943304
display id: range943304
version: 1
direction: direction/forward
top level: BBa_I0466
start: 1
end: 12
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator
creator: Jennifer Braff
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0032.E0040.B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_E0040
component: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
access: public
definition: GFP

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
access: public
definition: BBa_B0032

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
direction: direction/forward
top level: GFP report
start: 748
end: 827
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
direction: direction/forward
top level: GFP report
start: 20
end: 739
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
direction: direction/forward
top level: GFP report
start: 1
end: 13
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
direction: direction/forward
top level: GFP report
start: 836
end: 876
orientation: inline

BBa_K1092001
type: ComponentDefinition
persistent identity: BBa K1092001
display id: BBa_K1092001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7-RBS-QsrR
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1402_
m user id: 0
owner id: 16668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: T7-RBS-QsrR
mutable notes: T7-RBS-QsrR
mutable provenance: T7-RBS-QsrR
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1092000
component: BBa_K525998
sequence annotation: BBa_K525998
sequence annotation: BBa_K1092000
sequence: https://synbiohub.org/public/igem/BBa_K1092001_sequence/1

BBa_K1092000
type: Component
persistent identity: component2341225
display id: component2341225
version: 1
top level: BBa_K1092001
access: public
definition: https://synbiohub.org/public/igem/BBa_K1092000/1

BBa_K525998
type: Component
persistent identity: component2341224
display id: component2341224
version: 1
top level: BBa_K1092001
access: public
definition: https://synbiohub.org/public/igem/BBa_K525998/1

BBa_K525998
type: SequenceAnnotation
persistent identity: annotation2341224
display id: annotation2341224
version: 1
top level: BBa_K1092001
location: https://synbiohub.org/public/igem/BBa_K1092001/annotation2341224/range2341224/1
component: BBa_K525998

https://synbiohub.org/public/igem/BBa_K1092001/annotation2341224/range2341224/1
type: Range
persistent identity: range2341224
display id: range2341224
version: 1
direction: direction/reverse
top level: BBa_K1092001
start: 1
end: 32
orientation: inline

BBa_K1092000
type: SequenceAnnotation
persistent identity: annotation2341225
display id: annotation2341225
version: 1
top level: BBa_K1092001
location: https://synbiohub.org/public/igem/BBa_K1092001/annotation2341225/range2341225/1
component: BBa_K1092000

https://synbiohub.org/public/igem/BBa_K1092001/annotation2341225/range2341225/1
type: Range
persistent identity: range2341225
display id: range2341225
version: 1
direction: direction/reverse
top level: BBa_K1092001
start: 39
end: 377
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa C0050
display id: BBa_C0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage HK022 (+LVA?)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.
mutable notes: References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. Derived from STHK022N . Response from John Little (Arizona) regarding the start of HK022 cI From: <jlittle@email.arizona.edu> Date: Tue Jan 21, 2003 4:39:21 PM US/Eastern To: "Drew Endy" <endy@MIT.EDU> Subject: RE: hk022 cI start (naive question)? Hello Drew and Michael, I seriously doubt that the extra 27 aa are part of CI. It doesn't make sense in terms of the biology, for sure. In any case, the protein we characterized starts where Carlson and Little stated; as I recall, oR3 partially overlaps the start of cI. I have a vague memory of some possibly interesting biology, having to do with multicopy plasmids. I don't recall the findings themselves. Anyway, one possible explanation was that this extra N-terminal addition was made (e.g. from the pRE promoter, or from a message that arose from transcription around the entire plasmid), making a protein with altered functions. We never followed it up. Good luck John -- Original Message -- Date: Sun, 19 Jan 2003 15:26:26 -0500 Subject: hk022 cI start (naive question)? Cc: elowitm@rockefeller.edu To: jlittle@u.arizona.edu From: Drew Endy <endy@MIT.EDU> Hi John, I'm sitting here with Michael Elowitz and we're working through the sequence for HK022 cI. We noticed that the annotation from NC_002166 includes an "extra" 81 base pairs upstream of what we thought of as the actual start. It looks like this extra …
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0050
sequence annotation: Help:Barcodes
sequence annotation: cI HK022
sequence annotation: 2
sequence annotation: SsrA
sequence annotation: OR3 partial
sequence: https://synbiohub.org/public/igem/BBa_C0050_sequence/1

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation7033
display id: annotation7033
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1

https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
type: Range
persistent identity: range7033
display id: range7033
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213990
display id: annotation2213990
version: 1
top level: cI HK022
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1

https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
type: Range
persistent identity: range2213990
display id: range2213990
version: 1
direction: direction/forward
top level: cI HK022
start: 745
end: 769
orientation: inline

cI HK022
type: SequenceAnnotation
persistent identity: annotation1735
display id: annotation1735
version: 1
top level: cI HK022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
type: Range
persistent identity: range1735
display id: range1735
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 744
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1734
display id: annotation1734
version: 1
top level: cI HK022
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
type: Range
persistent identity: range1734
display id: range1734
version: 1
direction: direction/forward
top level: cI HK022
start: 739
end: 744
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1736
display id: annotation1736
version: 1
top level: cI HK022
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
type: Range
persistent identity: range1736
display id: range1736
version: 1
direction: direction/forward
top level: cI HK022
start: 706
end: 738
orientation: inline

OR3 partial
type: SequenceAnnotation
persistent identity: annotation1737
display id: annotation1737
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
type: Range
persistent identity: range1737
display id: range1737
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 8
orientation: inline

BBa_K137019
type: ComponentDefinition
persistent identity: BBa K137019
display id: BBa_K137019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL receiver + tet inverter + GFP
creator: Doug Tischer
created: 2008-07-15T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: A construct to make sure that luxR and effectively be coupled to the tetR inverter and shut down expression of GFP when AHL is present.
mutable notes: None.
mutable provenance: Biobricks registry.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_F2622
component: BBa_Q04400
component: BBa_I13504
sequence annotation: BBa_Q04400
sequence annotation: BBa_F2622
sequence annotation: BBa_I13504
sequence: https://synbiohub.org/public/igem/BBa_K137019_sequence/1

BBa_F2622
type: Component
persistent identity: component2305489
display id: component2305489
version: 1
top level: BBa_K137019
access: public
definition: BBa_F2622

BBa_Q04400
type: Component
persistent identity: component2305507
display id: component2305507
version: 1
top level: BBa_K137019
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04400/1

BBa_I13504
type: Component
persistent identity: component2305517
display id: component2305517
version: 1
top level: BBa_K137019
access: public
definition: BBa_I13504

BBa_Q04400
type: SequenceAnnotation
persistent identity: annotation2305507
display id: annotation2305507
version: 1
top level: BBa_K137019
location: https://synbiohub.org/public/igem/BBa_K137019/annotation2305507/range2305507/1
component: BBa_Q04400

https://synbiohub.org/public/igem/BBa_K137019/annotation2305507/range2305507/1
type: Range
persistent identity: range2305507
display id: range2305507
version: 1
direction: direction/reverse
top level: BBa_K137019
start: 1071
end: 1972
orientation: inline

BBa_F2622
type: SequenceAnnotation
persistent identity: annotation2305489
display id: annotation2305489
version: 1
top level: BBa_K137019
location: https://synbiohub.org/public/igem/BBa_K137019/annotation2305489/range2305489/1
component: BBa_F2622

https://synbiohub.org/public/igem/BBa_K137019/annotation2305489/range2305489/1
type: Range
persistent identity: range2305489
display id: range2305489
version: 1
direction: direction/reverse
top level: BBa_K137019
start: 1
end: 1062
orientation: inline

BBa_I13504
type: SequenceAnnotation
persistent identity: annotation2305517
display id: annotation2305517
version: 1
top level: BBa_K137019
location: https://synbiohub.org/public/igem/BBa_K137019/annotation2305517/range2305517/1
component: BBa_I13504

https://synbiohub.org/public/igem/BBa_K137019/annotation2305517/range2305517/1
type: Range
persistent identity: range2305517
display id: range2305517
version: 1
direction: direction/reverse
top level: BBa_K137019
start: 1981
end: 2855
orientation: inline

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (RhlR & C4-HSL regulated)
creator: Ronny Krashinsky
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RhlR
sequence annotation: -10
sequence annotation: -35
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
direction: direction/forward
top level: RhlR+C4
start: 1
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
direction: direction/forward
top level: RhlR+C4
start: 42
end: 47
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
direction: direction/forward
top level: RhlR+C4
start: 19
end: 24
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
direction: direction/forward
top level: RhlR+C4
start: 53
end: 53
orientation: inline

BBa_P0152
type: ComponentDefinition
persistent identity: BBa P0152
display id: BBa_P0152
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (434) [S0166]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI from 434. Used as the input section for Quad Part Inverter Q01520.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0012
component: BBa_C0052
component: BBa_B0031
sequence annotation: BBa_B0012
sequence annotation: BBa_C0052
sequence annotation: BBa_B0010
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_P0152_sequence/1

BBa_B0010
type: Component
persistent identity: component944552
display id: component944552
version: 1
top level: BBa_P0152
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component944562
display id: component944562
version: 1
top level: BBa_P0152
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0052
type: Component
persistent identity: component944545
display id: component944545
version: 1
top level: BBa_P0152
access: public
definition: cI 434

BBa_B0031
type: Component
persistent identity: component944535
display id: component944535
version: 1
top level: BBa_P0152
access: public
definition: BBa_B0031

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944562
display id: annotation944562
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944562/range944562/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0152/annotation944562/range944562/1
type: Range
persistent identity: range944562
display id: range944562
version: 1
direction: direction/forward
top level: BBa_P0152
start: 811
end: 851
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation944545
display id: annotation944545
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944545/range944545/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_P0152/annotation944545/range944545/1
type: Range
persistent identity: range944545
display id: range944545
version: 1
direction: direction/forward
top level: BBa_P0152
start: 21
end: 689
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944552
display id: annotation944552
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944552/range944552/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0152/annotation944552/range944552/1
type: Range
persistent identity: range944552
display id: range944552
version: 1
direction: direction/forward
top level: BBa_P0152
start: 723
end: 802
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944535
display id: annotation944535
version: 1
top level: BBa_P0152
location: https://synbiohub.org/public/igem/BBa_P0152/annotation944535/range944535/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0152/annotation944535/range944535/1
type: Range
persistent identity: range944535
display id: range944535
version: 1
direction: direction/forward
top level: BBa_P0152
start: 1
end: 14
orientation: inline

BBa_K1150038
type: ComponentDefinition
persistent identity: BBa K1150038
display id: BBa_K1150038
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: uniCAS RNAimer to target VEGF3
creator: Ilona Blank
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ..
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1150038_sequence/1

BBa_J23111
type: ComponentDefinition
persistent identity: BBa J23111
display id: BBa_J23111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23111_sequence/1

BBa_K118028
type: ComponentDefinition
persistent identity: BBa K118028
display id: BBa_K118028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: beta-glucosidase gene bglX (chu_2268) from Cytophaga hutchinsonii
creator: Chris French
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a beta-glucosidase gene (bglX, locus chu_2268) from the genome sequence of the cellulose-degrading Gram negative bacterium Cytophaga hutchinsonii.
mutable notes: No special considerations.
mutable provenance: Genome sequence of the cellulose-degrading Gram negative bacterium Cytophaga hutchinsonii.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: bglX coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118028_sequence/1

bglX coding sequence
type: SequenceAnnotation
persistent identity: annotation1985223
display id: annotation1985223
version: 1
top level: BBa_K118028
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118028/annotation1985223/range1985223/1

https://synbiohub.org/public/igem/BBa_K118028/annotation1985223/range1985223/1
type: Range
persistent identity: range1985223
display id: range1985223
version: 1
direction: direction/forward
top level: BBa_K118028
start: 1
end: 2277
orientation: inline

BBa_I712669
type: ComponentDefinition
persistent identity: BBa I712669
display id: BBa_I712669
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV - mCer
creator: Rok Gaber
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1856
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding sequence of GFP reporter protein under the control of CMV promoter.
mutable notes: AK3
mutable provenance: Ligation of two parts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J52642
component: BBa_I712004
sequence annotation: BBa_I712004
sequence annotation: BBa_J52642
sequence: https://synbiohub.org/public/igem/BBa_I712669_sequence/1

BBa_J52642
type: Component
persistent identity: component1953753
display id: component1953753
version: 1
top level: BBa_I712669
access: public
definition: https://synbiohub.org/public/igem/BBa_J52642/1

BBa_I712004
type: Component
persistent identity: component1953749
display id: component1953749
version: 1
top level: BBa_I712669
access: public
definition: https://synbiohub.org/public/igem/BBa_I712004/1

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation1953749
display id: annotation1953749
version: 1
top level: BBa_I712669
location: https://synbiohub.org/public/igem/BBa_I712669/annotation1953749/range1953749/1
component: BBa_I712004

https://synbiohub.org/public/igem/BBa_I712669/annotation1953749/range1953749/1
type: Range
persistent identity: range1953749
display id: range1953749
version: 1
direction: direction/reverse
top level: BBa_I712669
start: 1
end: 654
orientation: inline

BBa_J52642
type: SequenceAnnotation
persistent identity: annotation1953753
display id: annotation1953753
version: 1
top level: BBa_I712669
location: https://synbiohub.org/public/igem/BBa_I712669/annotation1953753/range1953753/1
component: BBa_J52642

https://synbiohub.org/public/igem/BBa_I712669/annotation1953753/range1953753/1
type: Range
persistent identity: range1953753
display id: range1953753
version: 1
direction: direction/reverse
top level: BBa_I712669
start: 661
end: 1380
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0034
display id: BBa_E0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: AAV
sequence: https://synbiohub.org/public/igem/BBa_E0034_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1245671
display id: annotation1245671
version: 1
top level: EYFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1

https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
type: Range
persistent identity: range1245671
display id: range1245671
version: 1
direction: direction/forward
top level: EYFP
start: 757
end: 762
orientation: inline

AAV
type: SequenceAnnotation
persistent identity: annotation1245672
display id: annotation1245672
version: 1
top level: EYFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1

https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
type: Range
persistent identity: range1245672
display id: range1245672
version: 1
direction: direction/forward
top level: EYFP
start: 718
end: 756
orientation: inline

BBa_K1150011
type: ComponentDefinition
persistent identity: BBa K1150011
display id: BBa_K1150011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40 promoter
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ..
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: SV40
sequence: https://synbiohub.org/public/igem/BBa_K1150011_sequence/1

SV40
type: SequenceAnnotation
persistent identity: annotation2347620
display id: annotation2347620
version: 1
top level: BBa_K1150011
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1150011/annotation2347620/range2347620/1

https://synbiohub.org/public/igem/BBa_K1150011/annotation2347620/range2347620/1
type: Range
persistent identity: range2347620
display id: range2347620
version: 1
direction: direction/forward
top level: BBa_K1150011
start: 1
end: 344
orientation: inline

BBa_K1150039
type: ComponentDefinition
persistent identity: BBa K1150039
display id: BBa_K1150039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: uniCAS RNAimer to target VEGF4
creator: Ilona Blank
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1150039_sequence/1

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: OR1
sequence annotation: -35
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

BBa_K1150007
type: ComponentDefinition
persistent identity: BBa K1150007
display id: BBa_K1150007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: COP1
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: COP1 is ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: COP1
sequence: https://synbiohub.org/public/igem/BBa_K1150007_sequence/1

COP1
type: SequenceAnnotation
persistent identity: annotation2347576
display id: annotation2347576
version: 1
top level: BBa_K1150007
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K1150007/annotation2347576/range2347576/1

https://synbiohub.org/public/igem/BBa_K1150007/annotation2347576/range2347576/1
type: Range
persistent identity: range2347576
display id: range2347576
version: 1
direction: direction/forward
top level: BBa_K1150007
start: 1
end: 1023
orientation: inline

BBa_J23115
type: ComponentDefinition
persistent identity: BBa J23115
display id: BBa_J23115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23115_sequence/1

BBa_J52022
type: ComponentDefinition
persistent identity: BBa J52022
display id: BBa_J52022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NfΚB-dnTraf6-linker-GFP
creator: Matej Skocaj
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _80_
m user id: 0
owner id: 857
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Part is composed of NfΚB promoter (recognation of this promoter for initiation of transcription depends on the presence of nuclear factor kappa B), TIR domain of Traf6 which is linked with 6 amino acid long linker to GFP.
mutable notes: None.
mutable provenance: NfΚB- dnTraf6- pDeNy GFP-
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J52010
component: BBa_J52021
sequence annotation: BBa_J52010
sequence annotation: BBa_J52021
sequence: https://synbiohub.org/public/igem/BBa_J52022_sequence/1

BBa_J52010
type: Component
persistent identity: component2218360
display id: component2218360
version: 1
top level: BBa_J52022
access: public
definition: https://synbiohub.org/public/igem/BBa_J52010/1

BBa_J52021
type: Component
persistent identity: component2218365
display id: component2218365
version: 1
top level: BBa_J52022
access: public
definition: https://synbiohub.org/public/igem/BBa_J52021/1

BBa_J52010
type: SequenceAnnotation
persistent identity: annotation2218360
display id: annotation2218360
version: 1
top level: BBa_J52022
location: https://synbiohub.org/public/igem/BBa_J52022/annotation2218360/range2218360/1
component: BBa_J52010

https://synbiohub.org/public/igem/BBa_J52022/annotation2218360/range2218360/1
type: Range
persistent identity: range2218360
display id: range2218360
version: 1
direction: direction/reverse
top level: BBa_J52022
start: 1
end: 814
orientation: inline

BBa_J52021
type: SequenceAnnotation
persistent identity: annotation2218365
display id: annotation2218365
version: 1
top level: BBa_J52022
location: https://synbiohub.org/public/igem/BBa_J52022/annotation2218365/range2218365/1
component: BBa_J52021

https://synbiohub.org/public/igem/BBa_J52022/annotation2218365/range2218365/1
type: Range
persistent identity: range2218365
display id: range2218365
version: 1
direction: direction/reverse
top level: BBa_J52022
start: 821
end: 2266
orientation: inline

BBa_S0105
type: ComponentDefinition
persistent identity: BBa S0105
display id: BBa_S0105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0053
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0053
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0053
sequence: https://synbiohub.org/public/igem/BBa_S0105_sequence/1

BBa_C0053
type: Component
persistent identity: component939789
display id: component939789
version: 1
top level: BBa_S0105
access: public
definition: c2 P22

BBa_B0034
type: Component
persistent identity: component939777
display id: component939777
version: 1
top level: BBa_S0105
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939777
display id: annotation939777
version: 1
top level: BBa_S0105
location: https://synbiohub.org/public/igem/BBa_S0105/annotation939777/range939777/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0105/annotation939777/range939777/1
type: Range
persistent identity: range939777
display id: range939777
version: 1
direction: direction/forward
top level: BBa_S0105
start: 1
end: 12
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation939789
display id: annotation939789
version: 1
top level: BBa_S0105
location: https://synbiohub.org/public/igem/BBa_S0105/annotation939789/range939789/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_S0105/annotation939789/range939789/1
type: Range
persistent identity: range939789
display id: range939789
version: 1
direction: direction/forward
top level: BBa_S0105
start: 19
end: 705
orientation: inline

BBa_S03119
type: ComponentDefinition
persistent identity: BBa S03119
display id: BBa_S03119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: --Specify Parts List--
creator: Randy Rettberg
created: 2004-04-19T11:00:00Z
modified: 2015-05-08T01:14:19Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from assembly 299
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0062
component: BBa_R0040
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_S03119_sequence/1

BBa_C0062
type: Component
persistent identity: component955049
display id: component955049
version: 1
top level: BBa_S03119
access: public
definition: luxr

BBa_R0040
type: Component
persistent identity: component955026
display id: component955026
version: 1
top level: BBa_S03119
access: public
definition: p(tetR)

BBa_B0010
type: Component
persistent identity: component955057
display id: component955057
version: 1
top level: BBa_S03119
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component955067
display id: component955067
version: 1
top level: BBa_S03119
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component955034
display id: component955034
version: 1
top level: BBa_S03119
access: public
definition: BBa_B0034

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation955049
display id: annotation955049
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955049/range955049/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_S03119/annotation955049/range955049/1
type: Range
persistent identity: range955049
display id: range955049
version: 1
direction: direction/forward
top level: BBa_S03119
start: 81
end: 836
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation955057
display id: annotation955057
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955057/range955057/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03119/annotation955057/range955057/1
type: Range
persistent identity: range955057
display id: range955057
version: 1
direction: direction/forward
top level: BBa_S03119
start: 870
end: 949
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation955067
display id: annotation955067
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955067/range955067/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03119/annotation955067/range955067/1
type: Range
persistent identity: range955067
display id: range955067
version: 1
direction: direction/forward
top level: BBa_S03119
start: 958
end: 998
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation955034
display id: annotation955034
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955034/range955034/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03119/annotation955034/range955034/1
type: Range
persistent identity: range955034
display id: range955034
version: 1
direction: direction/forward
top level: BBa_S03119
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation955026
display id: annotation955026
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955026/range955026/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S03119/annotation955026/range955026/1
type: Range
persistent identity: range955026
display id: range955026
version: 1
direction: direction/forward
top level: BBa_S03119
start: 1
end: 54
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R1062
display id: BBa_R1062
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (luxR and HSL regulated -- lux pR)
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"> Modified to comply with BBa_S0001: TTGACA-17N-GATACT
mutable provenance: V. fischeri
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: start
sequence annotation: BBa_R1062
sequence annotation: -35
sequence annotation: -10
sequence annotation: LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R1062_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2100
display id: annotation2100
version: 1
top level: lux pR
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1
type: Range
persistent identity: range2100
display id: range2100
version: 1
direction: direction/forward
top level: lux pR
start: 54
end: 54
orientation: inline

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation7077
display id: annotation7077
version: 1
top level: lux pR
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1

https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1
type: Range
persistent identity: range7077
display id: range7077
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 56
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2101
display id: annotation2101
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1
type: Range
persistent identity: range2101
display id: range2101
version: 1
direction: direction/forward
top level: lux pR
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2099
display id: annotation2099
version: 1
top level: lux pR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1
type: Range
persistent identity: range2099
display id: range2099
version: 1
direction: direction/forward
top level: lux pR
start: 42
end: 48
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2098
display id: annotation2098
version: 1
top level: lux pR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1

https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1
type: Range
persistent identity: range2098
display id: range2098
version: 1
direction: direction/forward
top level: lux pR
start: 1
end: 20
orientation: inline

BBa_J23108
type: ComponentDefinition
persistent identity: BBa J23108
display id: BBa_J23108
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23108_sequence/1

OmpR
type: ComponentDefinition
persistent identity: BBa R0084
display id: BBa_R0084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: F3 OmpR
sequence annotation: F1 OmpR
sequence annotation: -10
sequence annotation: -35
sequence annotation: F2 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0084_sequence/1

F3 OmpR
type: SequenceAnnotation
persistent identity: annotation301313
display id: annotation301313
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
type: Range
persistent identity: range301313
display id: range301313
version: 1
direction: direction/forward
top level: OmpR
start: 51
end: 68
orientation: inline

F1 OmpR
type: SequenceAnnotation
persistent identity: annotation301308
display id: annotation301308
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
type: Range
persistent identity: range301308
display id: range301308
version: 1
direction: direction/forward
top level: OmpR
start: 11
end: 28
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301318
display id: annotation301318
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
type: Range
persistent identity: range301318
display id: range301318
version: 1
direction: direction/forward
top level: OmpR
start: 88
end: 93
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301317
display id: annotation301317
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
type: Range
persistent identity: range301317
display id: range301317
version: 1
direction: direction/forward
top level: OmpR
start: 73
end: 78
orientation: inline

F2 OmpR
type: SequenceAnnotation
persistent identity: annotation301309
display id: annotation301309
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
type: Range
persistent identity: range301309
display id: range301309
version: 1
direction: direction/forward
top level: OmpR
start: 31
end: 48
orientation: inline

BBa_B0024
type: ComponentDefinition
persistent identity: BBa B0024
display id: BBa_B0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0012-B0011), reversed
creator: Caitlin Conboy
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0024_sequence/1

BBa_K1166002
type: ComponentDefinition
persistent identity: BBa K1166002
display id: BBa_K1166002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HlyA-tag+Secretion system
creator: Luis Mario Leal Garza & Luis Fernando Camarillo Guerrero
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1478_
m user id: 0
owner id: 13053
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This secretion device includes all the necessary elements (linker-HlyA secretion signal peptide and the expression cassette for HlyB and HlyD) for the secretion of expression cassettes cloned in the 5??? terminal of the device. This secretion device is usable by Silver standard fusion in its 5??? end for proteins with no stop codon. Codes for both the proteins HlyB and HlyD (native from uropathogenic strains of E.coli), which, along with the constitutively expressed TolC protein, form a secretion channel. This secretion system recognizes proteins tagged with the C-terminal secretion signal peptide HlyA, and secretes them to the exterior of the bacteria.
mutable notes: HlyA C terminal part has a 5' section compatible with Silver Fusion standard. Downstream, HlyB and HlyD are both under the AraC/pBAD promoter to be induced by arabinose.
mutable provenance: The HlyB and HlyD sequences come from the parts registry, while the HlyA from the registry had mayor problems, so we took it from the genomic sequence.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: BBa_B0015
sequence annotation: stop
sequence annotation: HlyB
sequence annotation: BBa_B0034
sequence annotation: HlyA
sequence annotation: spacer
sequence annotation: AraC+pBAD
sequence annotation: Linker
sequence annotation: HlyD
sequence: https://synbiohub.org/public/igem/BBa_K1166002_sequence/1

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2351373
display id: annotation2351373
version: 1
top level: BBa_K1166002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351373/range2351373/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351373/range2351373/1
type: Range
persistent identity: range2351373
display id: range2351373
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 5159
end: 5287
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2351346
display id: annotation2351346
version: 1
top level: BBa_K1166002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351346/range2351346/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351346/range2351346/1
type: Range
persistent identity: range2351346
display id: range2351346
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 211
end: 216
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2351351
display id: annotation2351351
version: 1
top level: BBa_K1166002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351351/range2351351/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351351/range2351351/1
type: Range
persistent identity: range2351351
display id: range2351351
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 1574
end: 3694
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351353
display id: annotation2351353
version: 1
top level: BBa_K1166002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351353/range2351353/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351353/range2351353/1
type: Range
persistent identity: range2351353
display id: range2351353
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 3701
end: 3712
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2351347
display id: annotation2351347
version: 1
top level: BBa_K1166002
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351347/range2351347/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351347/range2351347/1
type: Range
persistent identity: range2351347
display id: range2351347
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 217
end: 345
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2351356
display id: annotation2351356
version: 1
top level: BBa_K1166002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351356/range2351356/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351356/range2351356/1
type: Range
persistent identity: range2351356
display id: range2351356
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 5153
end: 5158
orientation: inline

HlyA
type: SequenceAnnotation
persistent identity: annotation2351345
display id: annotation2351345
version: 1
top level: BBa_K1166002
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351345/range2351345/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351345/range2351345/1
type: Range
persistent identity: range2351345
display id: range2351345
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 31
end: 210
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation2351354
display id: annotation2351354
version: 1
top level: BBa_K1166002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351354/range2351354/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351354/range2351354/1
type: Range
persistent identity: range2351354
display id: range2351354
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 3713
end: 3718
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2351349
display id: annotation2351349
version: 1
top level: BBa_K1166002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351349/range2351349/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351349/range2351349/1
type: Range
persistent identity: range2351349
display id: range2351349
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 1556
end: 1567
orientation: inline

AraC+pBAD
type: SequenceAnnotation
persistent identity: annotation2351348
display id: annotation2351348
version: 1
top level: BBa_K1166002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351348/range2351348/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351348/range2351348/1
type: Range
persistent identity: range2351348
display id: range2351348
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 346
end: 1555
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2351352
display id: annotation2351352
version: 1
top level: BBa_K1166002
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351352/range2351352/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351352/range2351352/1
type: Range
persistent identity: range2351352
display id: range2351352
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 3695
end: 3700
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation2351350
display id: annotation2351350
version: 1
top level: BBa_K1166002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351350/range2351350/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351350/range2351350/1
type: Range
persistent identity: range2351350
display id: range2351350
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 1568
end: 1573
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2351344
display id: annotation2351344
version: 1
top level: BBa_K1166002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351344/range2351344/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351344/range2351344/1
type: Range
persistent identity: range2351344
display id: range2351344
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 1
end: 30
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2351355
display id: annotation2351355
version: 1
top level: BBa_K1166002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1166002/annotation2351355/range2351355/1

https://synbiohub.org/public/igem/BBa_K1166002/annotation2351355/range2351355/1
type: Range
persistent identity: range2351355
display id: range2351355
version: 1
direction: direction/forward
top level: BBa_K1166002
start: 3719
end: 5152
orientation: inline

BBa_K1150006
type: ComponentDefinition
persistent identity: BBa K1150006
display id: BBa_K1150006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: UVR8
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: UVR8 ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: UVR8
sequence: https://synbiohub.org/public/igem/BBa_K1150006_sequence/1

UVR8
type: SequenceAnnotation
persistent identity: annotation2347612
display id: annotation2347612
version: 1
top level: BBa_K1150006
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1150006/annotation2347612/range2347612/1

https://synbiohub.org/public/igem/BBa_K1150006/annotation2347612/range2347612/1
type: Range
persistent identity: range2347612
display id: range2347612
version: 1
direction: direction/forward
top level: BBa_K1150006
start: 1
end: 1110
orientation: inline

BBa_K118005
type: ComponentDefinition
persistent identity: BBa K118005
display id: BBa_K118005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtI (phytoene dehydrogenase)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087) preceded by an E. coli synthetic ribosome binding site (rbs). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
mutable provenance: rbs: Escherichia coli JM109 genomic DNA. crtI: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118003
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118003
sequence: https://synbiohub.org/public/igem/BBa_K118005_sequence/1

BBa_K118003
type: Component
persistent identity: component1970360
display id: component1970360
version: 1
top level: BBa_K118005
access: public
definition: https://synbiohub.org/public/igem/BBa_K118003/1

BBa_J15001
type: Component
persistent identity: component1970357
display id: component1970357
version: 1
top level: BBa_K118005
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970357
display id: annotation1970357
version: 1
top level: BBa_K118005
location: https://synbiohub.org/public/igem/BBa_K118005/annotation1970357/range1970357/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118005/annotation1970357/range1970357/1
type: Range
persistent identity: range1970357
display id: range1970357
version: 1
direction: direction/reverse
top level: BBa_K118005
start: 1
end: 10
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation1970360
display id: annotation1970360
version: 1
top level: BBa_K118005
location: https://synbiohub.org/public/igem/BBa_K118005/annotation1970360/range1970360/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K118005/annotation1970360/range1970360/1
type: Range
persistent identity: range1970360
display id: range1970360
version: 1
direction: direction/reverse
top level: BBa_K118005
start: 17
end: 1498
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tetracycline repressor from transposon Tn10 (+LVA)
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetR
sequence annotation: Help:Barcodes
sequence annotation: SsrA
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
direction: direction/forward
top level: tetR
start: 4
end: 620
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
direction: direction/forward
top level: tetR
start: 661
end: 685
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
direction: direction/forward
top level: tetR
start: 621
end: 654
orientation: inline

BBa_J23103
type: ComponentDefinition
persistent identity: BBa J23103
display id: BBa_J23103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23103_sequence/1

BBa_C0056
type: ComponentDefinition
persistent identity: BBa C0056
display id: BBa_C0056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (no LVA)
creator: Reshma Shetty
created: 2004-09-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Removed LVA tag from BBa_C0052 via PCR. In pSB1AK3 currently.
mutable provenance: Removed LVA tag from BBa_C0052
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI 434
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_C0056_sequence/1

cI 434
type: SequenceAnnotation
persistent identity: annotation1063931
display id: annotation1063931
version: 1
top level: BBa_C0056
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1

https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1
type: Range
persistent identity: range1063931
display id: range1063931
version: 1
direction: direction/forward
top level: BBa_C0056
start: 1
end: 636
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1063930
display id: annotation1063930
version: 1
top level: BBa_C0056
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1

https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1
type: Range
persistent identity: range1063930
display id: range1063930
version: 1
direction: direction/forward
top level: BBa_C0056
start: 631
end: 636
orientation: inline

BBa_I751100
type: ComponentDefinition
persistent identity: BBa I751100
display id: BBa_I751100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J54140 delPr
creator: Kenichiro Iwasaki
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A-4 delta P
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: J54033
sequence annotation: R0063
sequence annotation: BamHI
sequence annotation: J54025
sequence annotation: B0010
sequence annotation: B0012
sequence annotation: C0062
sequence annotation: BglII
sequence annotation: B0034
sequence annotation: MluI
sequence annotation: B0030
sequence annotation: SalI
sequence annotation: E0040
sequence: https://synbiohub.org/public/igem/BBa_I751100_sequence/1

J54033
type: SequenceAnnotation
persistent identity: annotation1954390
display id: annotation1954390
version: 1
top level: BBa_I751100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954390/range1954390/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954390/range1954390/1
type: Range
persistent identity: range1954390
display id: range1954390
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1104
end: 1123
orientation: inline

R0063
type: SequenceAnnotation
persistent identity: annotation1954380
display id: annotation1954380
version: 1
top level: BBa_I751100
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954380/range1954380/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954380/range1954380/1
type: Range
persistent identity: range1954380
display id: range1954380
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1
end: 151
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1954392
display id: annotation1954392
version: 1
top level: BBa_I751100
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954392/range1954392/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954392/range1954392/1
type: Range
persistent identity: range1954392
display id: range1954392
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1118
end: 1123
orientation: inline

J54025
type: SequenceAnnotation
persistent identity: annotation1954395
display id: annotation1954395
version: 1
top level: BBa_I751100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954395/range1954395/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954395/range1954395/1
type: Range
persistent identity: range1954395
display id: range1954395
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1130
end: 1149
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation1954388
display id: annotation1954388
version: 1
top level: BBa_I751100
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954388/range1954388/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954388/range1954388/1
type: Range
persistent identity: range1954388
display id: range1954388
version: 1
direction: direction/forward
top level: BBa_I751100
start: 967
end: 1046
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation1954389
display id: annotation1954389
version: 1
top level: BBa_I751100
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954389/range1954389/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954389/range1954389/1
type: Range
persistent identity: range1954389
display id: range1954389
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1055
end: 1095
orientation: inline

C0062
type: SequenceAnnotation
persistent identity: annotation1954385
display id: annotation1954385
version: 1
top level: BBa_I751100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954385/range1954385/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954385/range1954385/1
type: Range
persistent identity: range1954385
display id: range1954385
version: 1
direction: direction/forward
top level: BBa_I751100
start: 178
end: 958
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1954393
display id: annotation1954393
version: 1
top level: BBa_I751100
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954393/range1954393/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954393/range1954393/1
type: Range
persistent identity: range1954393
display id: range1954393
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1130
end: 1135
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1954382
display id: annotation1954382
version: 1
top level: BBa_I751100
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954382/range1954382/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954382/range1954382/1
type: Range
persistent identity: range1954382
display id: range1954382
version: 1
direction: direction/forward
top level: BBa_I751100
start: 160
end: 171
orientation: inline

MluI
type: SequenceAnnotation
persistent identity: annotation1954394
display id: annotation1954394
version: 1
top level: BBa_I751100
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954394/range1954394/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954394/range1954394/1
type: Range
persistent identity: range1954394
display id: range1954394
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1144
end: 1149
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation1954399
display id: annotation1954399
version: 1
top level: BBa_I751100
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954399/range1954399/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954399/range1954399/1
type: Range
persistent identity: range1954399
display id: range1954399
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1995
end: 2035
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation1954396
display id: annotation1954396
version: 1
top level: BBa_I751100
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954396/range1954396/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954396/range1954396/1
type: Range
persistent identity: range1954396
display id: range1954396
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1158
end: 1172
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation1954391
display id: annotation1954391
version: 1
top level: BBa_I751100
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954391/range1954391/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954391/range1954391/1
type: Range
persistent identity: range1954391
display id: range1954391
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1104
end: 1109
orientation: inline

E0040
type: SequenceAnnotation
persistent identity: annotation1954397
display id: annotation1954397
version: 1
top level: BBa_I751100
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954397/range1954397/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954397/range1954397/1
type: Range
persistent identity: range1954397
display id: range1954397
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1179
end: 1897
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation1954398
display id: annotation1954398
version: 1
top level: BBa_I751100
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751100/annotation1954398/range1954398/1

https://synbiohub.org/public/igem/BBa_I751100/annotation1954398/range1954398/1
type: Range
persistent identity: range1954398
display id: range1954398
version: 1
direction: direction/forward
top level: BBa_I751100
start: 1907
end: 1985
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0024
display id: BBa_E0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0024_sequence/1

BBa_I746902
type: ComponentDefinition
persistent identity: BBa I746902
display id: BBa_I746902
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pbad promoter driving 6-his tagged mut3 GFP
creator: Stefan Milde
created: 2008-09-30T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is used for purification of mut3 GFP via its 6-his tag. It is driven by the arabinose - sensitive pbad promoter. Induction should be with 1mM arabinose for overnight growth of a 50ml (or more) culture for purification the next day.
mutable notes: no special considerations.
mutable provenance: The part was assembled from intermediates via standard assembly.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I746918
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_I0500
sequence annotation: BBa_I0500
sequence annotation: BBa_B0010
sequence annotation: BBa_I746918
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I746902_sequence/1

BBa_I746918
type: Component
persistent identity: component1978095
display id: component1978095
version: 1
top level: BBa_I746902
access: public
definition: https://synbiohub.org/public/igem/BBa_I746918/1

BBa_B0034
type: Component
persistent identity: component1978090
display id: component1978090
version: 1
top level: BBa_I746902
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component1978096
display id: component1978096
version: 1
top level: BBa_I746902
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component1978098
display id: component1978098
version: 1
top level: BBa_I746902
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I0500
type: Component
persistent identity: component1978088
display id: component1978088
version: 1
top level: BBa_I746902
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1978088
display id: annotation1978088
version: 1
top level: BBa_I746902
location: https://synbiohub.org/public/igem/BBa_I746902/annotation1978088/range1978088/1
component: BBa_I0500

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type: Range
persistent identity: range1978088
display id: range1978088
version: 1
direction: direction/reverse
top level: BBa_I746902
start: 1
end: 1210
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1978096
display id: annotation1978096
version: 1
top level: BBa_I746902
location: https://synbiohub.org/public/igem/BBa_I746902/annotation1978096/range1978096/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746902/annotation1978096/range1978096/1
type: Range
persistent identity: range1978096
display id: range1978096
version: 1
direction: direction/forward
top level: BBa_I746902
start: 1983
end: 2062
orientation: inline

BBa_I746918
type: SequenceAnnotation
persistent identity: annotation1978095
display id: annotation1978095
version: 1
top level: BBa_I746902
location: https://synbiohub.org/public/igem/BBa_I746902/annotation1978095/range1978095/1
component: BBa_I746918

https://synbiohub.org/public/igem/BBa_I746902/annotation1978095/range1978095/1
type: Range
persistent identity: range1978095
display id: range1978095
version: 1
direction: direction/reverse
top level: BBa_I746902
start: 1237
end: 1974
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1978098
display id: annotation1978098
version: 1
top level: BBa_I746902
location: https://synbiohub.org/public/igem/BBa_I746902/annotation1978098/range1978098/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746902/annotation1978098/range1978098/1
type: Range
persistent identity: range1978098
display id: range1978098
version: 1
direction: direction/forward
top level: BBa_I746902
start: 2071
end: 2111
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1978090
display id: annotation1978090
version: 1
top level: BBa_I746902
location: https://synbiohub.org/public/igem/BBa_I746902/annotation1978090/range1978090/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746902/annotation1978090/range1978090/1
type: Range
persistent identity: range1978090
display id: range1978090
version: 1
direction: direction/reverse
top level: BBa_I746902
start: 1219
end: 1230
orientation: inline

BBa_J23105
type: ComponentDefinition
persistent identity: BBa J23105
display id: BBa_J23105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23105_sequence/1

BBa_P0312
type: ComponentDefinition
persistent identity: BBa P0312
display id: BBa_P0312
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> LacI [S0156]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0012
component: BBa_B0012
component: BBa_B0010
component: BBa_B0033
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0012
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_P0312_sequence/1

BBa_C0012
type: Component
persistent identity: component944617
display id: component944617
version: 1
top level: BBa_P0312
access: public
definition: lacI

BBa_B0012
type: Component
persistent identity: component944634
display id: component944634
version: 1
top level: BBa_P0312
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944624
display id: component944624
version: 1
top level: BBa_P0312
access: public
definition: BBa_B0010

BBa_B0033
type: Component
persistent identity: component944606
display id: component944606
version: 1
top level: BBa_P0312
access: public
definition: BBa_B0033

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944634
display id: annotation944634
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944634/range944634/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0312/annotation944634/range944634/1
type: Range
persistent identity: range944634
display id: range944634
version: 1
direction: direction/forward
top level: BBa_P0312
start: 1267
end: 1307
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944624
display id: annotation944624
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944624/range944624/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0312/annotation944624/range944624/1
type: Range
persistent identity: range944624
display id: range944624
version: 1
direction: direction/forward
top level: BBa_P0312
start: 1179
end: 1258
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation944617
display id: annotation944617
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944617/range944617/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_P0312/annotation944617/range944617/1
type: Range
persistent identity: range944617
display id: range944617
version: 1
direction: direction/forward
top level: BBa_P0312
start: 18
end: 1145
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation944606
display id: annotation944606
version: 1
top level: BBa_P0312
location: https://synbiohub.org/public/igem/BBa_P0312/annotation944606/range944606/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_P0312/annotation944606/range944606/1
type: Range
persistent identity: range944606
display id: range944606
version: 1
direction: direction/forward
top level: BBa_P0312
start: 1
end: 11
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxICDABEG (+/-)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri AF170104 .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
direction: direction/forward
top level: BBa_B0011
start: 13
end: 35
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
direction: direction/forward
top level: BBa_B0011
start: 1
end: 46
orientation: inline

BBa_I13507
type: ComponentDefinition
persistent identity: BBa I13507
display id: BBa_I13507
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_I13507_sequence/1

BBa_B0034
type: Component
persistent identity: component1524815
display id: component1524815
version: 1
top level: BBa_I13507
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component1524828
display id: component1524828
version: 1
top level: BBa_I13507
access: public
definition: BBa_B0010

BBa_E1010
type: Component
persistent identity: component1524822
display id: component1524822
version: 1
top level: BBa_I13507
access: public
definition: https://synbiohub.org/public/igem/BBa_E1010/1

BBa_B0012
type: Component
persistent identity: component1524838
display id: component1524838
version: 1
top level: BBa_I13507
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1524815
display id: annotation1524815
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
type: Range
persistent identity: range1524815
display id: range1524815
version: 1
direction: direction/forward
top level: BBa_I13507
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1524838
display id: annotation1524838
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
type: Range
persistent identity: range1524838
display id: range1524838
version: 1
direction: direction/forward
top level: BBa_I13507
start: 821
end: 861
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1524828
display id: annotation1524828
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
type: Range
persistent identity: range1524828
display id: range1524828
version: 1
direction: direction/forward
top level: BBa_I13507
start: 733
end: 812
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1524822
display id: annotation1524822
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
type: Range
persistent identity: range1524822
display id: range1524822
version: 1
direction: direction/forward
top level: BBa_I13507
start: 19
end: 699
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: YFP (LVA)
sequence annotation: 2
sequence annotation: BBa_E0032
sequence annotation: SsrA
sequence annotation: A (Q->L)
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 762
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
direction: direction/forward
top level: YFP
start: 757
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
direction: direction/forward
top level: YFP
start: 1
end: 762
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
direction: direction/forward
top level: YFP
start: 718
end: 756
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
direction: direction/forward
top level: YFP
start: 242
end: 242
orientation: inline

spacer
type: ComponentDefinition
persistent identity: BBa B0040
display id: BBa_B0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spacer.1 (generic)
creator: Vinay S. Mahajan, Brian Chow, Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.
mutable notes: The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the Random DNA Generator engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other E.coli sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur): T->A @ 85 T->A @ 42 C->T @ 79 A->T @ 64 A->T @ 31 T->A @ 34 C->A @ 37 Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences. In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
mutable provenance: Randomly generated and optimized for several parameters (see Design notes).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Spacer-1
sequence annotation: BBa_B0040
sequence: https://synbiohub.org/public/igem/BBa_B0040_sequence/1

Spacer-1
type: SequenceAnnotation
persistent identity: annotation1721
display id: annotation1721
version: 1
top level: spacer
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1

https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
type: Range
persistent identity: range1721
display id: range1721
version: 1
direction: direction/forward
top level: spacer
start: 1
end: 70
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation7030
display id: annotation7030
version: 1
top level: spacer
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1

https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
type: Range
persistent identity: range7030
display id: range7030
version: 1
direction: direction/forward
top level: spacer
start: 1
end: 70
orientation: inline

BBa_I13504
type: ComponentDefinition
persistent identity: BBa I13504
display id: BBa_I13504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13504_sequence/1

BBa_B0012
type: Component
persistent identity: component1505084
display id: component1505084
version: 1
top level: BBa_I13504
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1505067
display id: component1505067
version: 1
top level: BBa_I13504
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component1505074
display id: component1505074
version: 1
top level: BBa_I13504
access: public
definition: BBa_B0010

BBa_E0040
type: Component
persistent identity: component1505069
display id: component1505069
version: 1
top level: BBa_I13504
access: public
definition: GFP

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505069
display id: annotation1505069
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
type: Range
persistent identity: range1505069
display id: range1505069
version: 1
direction: direction/forward
top level: BBa_I13504
start: 19
end: 738
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505067
display id: annotation1505067
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
type: Range
persistent identity: range1505067
display id: range1505067
version: 1
direction: direction/forward
top level: BBa_I13504
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505074
display id: annotation1505074
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
type: Range
persistent identity: range1505074
display id: range1505074
version: 1
direction: direction/forward
top level: BBa_I13504
start: 747
end: 826
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505084
display id: annotation1505084
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
type: Range
persistent identity: range1505084
display id: range1505084
version: 1
direction: direction/forward
top level: BBa_I13504
start: 835
end: 875
orientation: inline

BBa_K1218011
type: ComponentDefinition
persistent identity: BBa K1218011
display id: BBa_K1218011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Cas9
creator: Sophia Liang
created: 2013-08-28T11:00:00Z
modified: 2015-05-08T01:09:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1532_
m user id: 0
owner id: 17300
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: CRISPR-Cas is a bacterial immune system that remembers and targets foreign viral DNA by storing DNA sequences, or spacers, between clustered regularly interspaced short palindromic repeats (CRISPRs). RNA transcripts of the spacers are then used to sense homologous DNA, which is cleaved by CRISPR-associated (Cas) proteins. This part codes for the tracrRNA, Cas9 protein, and minimal CRISPR array of a type II CRISPR-Cas system. The CRISPR array includes two CRISPR repeats separated by a spacer with two BsaI sites, which allow for the easy cloning of new spacers.
mutable notes: Mutagenesis to remove internal EcoRI site
mutable provenance: Streptococcus pyogenes
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1218011_sequence/1

BBa_I13401
type: ComponentDefinition
persistent identity: BBa I13401
display id: BBa_I13401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP reporter for RHS of library test constructs
creator: jasonk
created: 2005-04-05T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 2
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part will be suffixed to a promoter.RBS library. The purpose of the experiment is to make a first pass at a library-based construction step to identify efficiency issues and also to examine maxPOPS/RIPS level.
mutable notes: This part was built by Jen as an intermediate to their nuts and bolts parts.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13401_sequence/1

BBa_E0040
type: Component
persistent identity: component1476713
display id: component1476713
version: 1
top level: BBa_I13401
access: public
definition: GFP

BBa_B0012
type: Component
persistent identity: component1476728
display id: component1476728
version: 1
top level: BBa_I13401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1476718
display id: component1476718
version: 1
top level: BBa_I13401
access: public
definition: BBa_B0010

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1476728
display id: annotation1476728
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476728/range1476728/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13401/annotation1476728/range1476728/1
type: Range
persistent identity: range1476728
display id: range1476728
version: 1
direction: direction/forward
top level: BBa_I13401
start: 817
end: 857
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1476718
display id: annotation1476718
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476718/range1476718/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13401/annotation1476718/range1476718/1
type: Range
persistent identity: range1476718
display id: range1476718
version: 1
direction: direction/forward
top level: BBa_I13401
start: 729
end: 808
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1476713
display id: annotation1476713
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476713/range1476713/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13401/annotation1476713/range1476713/1
type: Range
persistent identity: range1476713
display id: range1476713
version: 1
direction: direction/forward
top level: BBa_I13401
start: 1
end: 720
orientation: inline

BBa_K1031410
type: ComponentDefinition
persistent identity: BBa K1031410
display id: BBa_K1031410
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HcaR-Terminator
creator: He Shuaixin
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:08:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1338_
m user id: 0
owner id: 15843
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: HcaR-TT
mutable notes: molecular cloning
mutable provenance: PCR genome E.coli
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: HcaR
sequence: https://synbiohub.org/public/igem/BBa_K1031410_sequence/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2357450
display id: annotation2357450
version: 1
top level: BBa_K1031410
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357450/range2357450/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357450/range2357450/1
type: Range
persistent identity: range2357450
display id: range2357450
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 1
end: 12
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2357452
display id: annotation2357452
version: 1
top level: BBa_K1031410
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357452/range2357452/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357452/range2357452/1
type: Range
persistent identity: range2357452
display id: range2357452
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 918
end: 1046
orientation: inline

HcaR
type: SequenceAnnotation
persistent identity: annotation2357451
display id: annotation2357451
version: 1
top level: BBa_K1031410
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1031410/annotation2357451/range2357451/1

https://synbiohub.org/public/igem/BBa_K1031410/annotation2357451/range2357451/1
type: Range
persistent identity: range2357451
display id: range2357451
version: 1
direction: direction/forward
top level: BBa_K1031410
start: 19
end: 909
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T1 from E. coli rrnB
creator: Randy Rettberg
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
direction: direction/forward
top level: BBa_B0010
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
direction: direction/forward
top level: BBa_B0010
start: 12
end: 55
orientation: inline

SbpA | CFP
type: ComponentDefinition
persistent identity: BBa K525406
display id: BBa_K525406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and mCerulean
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T09:45:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer SgsE and mCerulean
mutable notes: Fusion Protein of BBa_K525403 and BBa_J18930
mutable provenance: Fusion Protein of S-Layer SgsE and mCerulean
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: lac operator
sequence annotation: AgeI
sequence annotation: RBS
sequence annotation: Freiburg scar
sequence annotation: T7 promotoer
sequence annotation: K525401
sequence annotation: SbpA domain
sequence annotation: ATG
sequence annotation: NgoMIV
sequence annotation: mCerulean (CFP)
sequence annotation: TAA
sequence annotation: J18930
sequence annotation: B0034
sequence: https://synbiohub.org/public/igem/BBa_K525406_sequence/1

lac operator
type: SequenceAnnotation
persistent identity: annotation2129917
display id: annotation2129917
version: 1
top level: SbpA | CFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129917/range2129917/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129917/range2129917/1
type: Range
persistent identity: range2129917
display id: range2129917
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 20
end: 44
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129928
display id: annotation2129928
version: 1
top level: SbpA | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129928/range2129928/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129928/range2129928/1
type: Range
persistent identity: range2129928
display id: range2129928
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3913
end: 3918
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129919
display id: annotation2129919
version: 1
top level: SbpA | CFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129919/range2129919/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129919/range2129919/1
type: Range
persistent identity: range2129919
display id: range2129919
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 56
end: 59
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2129924
display id: annotation2129924
version: 1
top level: SbpA | CFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129924/range2129924/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129924/range2129924/1
type: Range
persistent identity: range2129924
display id: range2129924
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3193
end: 3198
orientation: inline

T7 promotoer
type: SequenceAnnotation
persistent identity: annotation2129916
display id: annotation2129916
version: 1
top level: SbpA | CFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129916/range2129916/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129916/range2129916/1
type: Range
persistent identity: range2129916
display id: range2129916
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 1
end: 20
orientation: inline

K525401
type: SequenceAnnotation
persistent identity: annotation2129920
display id: annotation2129920
version: 1
top level: SbpA | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129920/range2129920/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129920/range2129920/1
type: Range
persistent identity: range2129920
display id: range2129920
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 73
end: 3192
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2129921
display id: annotation2129921
version: 1
top level: SbpA | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129921/range2129921/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129921/range2129921/1
type: Range
persistent identity: range2129921
display id: range2129921
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 73
end: 3192
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129922
display id: annotation2129922
version: 1
top level: SbpA | CFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129922/range2129922/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129922/range2129922/1
type: Range
persistent identity: range2129922
display id: range2129922
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 73
end: 75
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129923
display id: annotation2129923
version: 1
top level: SbpA | CFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129923/range2129923/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129923/range2129923/1
type: Range
persistent identity: range2129923
display id: range2129923
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 76
end: 81
orientation: inline

mCerulean (CFP)
type: SequenceAnnotation
persistent identity: annotation2129927
display id: annotation2129927
version: 1
top level: SbpA | CFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129927/range2129927/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129927/range2129927/1
type: Range
persistent identity: range2129927
display id: range2129927
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3199
end: 3921
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129926
display id: annotation2129926
version: 1
top level: SbpA | CFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129926/range2129926/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129926/range2129926/1
type: Range
persistent identity: range2129926
display id: range2129926
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3919
end: 3921
orientation: inline

J18930
type: SequenceAnnotation
persistent identity: annotation2129925
display id: annotation2129925
version: 1
top level: SbpA | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129925/range2129925/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129925/range2129925/1
type: Range
persistent identity: range2129925
display id: range2129925
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 3199
end: 3921
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2129918
display id: annotation2129918
version: 1
top level: SbpA | CFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525406/annotation2129918/range2129918/1

https://synbiohub.org/public/igem/BBa_K525406/annotation2129918/range2129918/1
type: Range
persistent identity: range2129918
display id: range2129918
version: 1
direction: direction/forward
top level: SbpA | CFP
start: 53
end: 64
orientation: inline

KaiA_KaiC_
type: ComponentDefinition
persistent identity: BBa J36335
display id: BBa_J36335
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac+RBS+KaiA+Lac+RBS+KaiC
creator: Zhipeng Sun, Hetmann Hsieh, Jeffrey Lau, David Ramos
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _65_
m user id: 0
owner id: 1189
owning group id: 65
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Composite of J36831 and J36834. KaiA and KaiC protein expression in the ratio 1:2 by amount or ~1:4 by mass.
mutable notes: -none-
mutable provenance: Formed by J36831 /SP ligated with J36834 /XP. see http://openwetware.org/wiki/IGEM:Harvard/2006/Cyanobacteria for more information; antiquated name is "J36022".
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_B0034
component: BBa_J36801
component: BBa_J36804
sequence annotation: BBa_B0034
sequence annotation: BBa_J36801
sequence annotation: BBa_R0010
sequence annotation: BBa_J36804
sequence: https://synbiohub.org/public/igem/BBa_J36335_sequence/1

BBa_R0010
type: Component
persistent identity: component1909056
display id: component1909056
version: 1
top level: KaiA_KaiC_
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_B0034
type: Component
persistent identity: component1909054
display id: component1909054
version: 1
top level: KaiA_KaiC_
access: public
definition: BBa_B0034

BBa_R0010
type: Component
persistent identity: component1909046
display id: component1909046
version: 1
top level: KaiA_KaiC_
access: public
definition: https://synbiohub.org/public/igem/BBa_R0010/1

BBa_J36801
type: Component
persistent identity: component1909055
display id: component1909055
version: 1
top level: KaiA_KaiC_
access: public
definition: https://synbiohub.org/public/igem/BBa_J36801/1

BBa_J36804
type: Component
persistent identity: component1909065
display id: component1909065
version: 1
top level: KaiA_KaiC_
access: public
definition: https://synbiohub.org/public/igem/BBa_J36804/1

BBa_B0034
type: Component
persistent identity: component1909064
display id: component1909064
version: 1
top level: KaiA_KaiC_
access: public
definition: BBa_B0034

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1909054
display id: annotation1909054
version: 1
top level: KaiA_KaiC_
location: https://synbiohub.org/public/igem/BBa_J36335/annotation1909054/range1909054/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J36335/annotation1909054/range1909054/1
type: Range
persistent identity: range1909054
display id: range1909054
version: 1
direction: direction/reverse
top level: KaiA_KaiC_
start: 209
end: 220
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1909064
display id: annotation1909064
version: 1
top level: KaiA_KaiC_
location: https://synbiohub.org/public/igem/BBa_J36335/annotation1909064/range1909064/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J36335/annotation1909064/range1909064/1
type: Range
persistent identity: range1909064
display id: range1909064
version: 1
direction: direction/reverse
top level: KaiA_KaiC_
start: 1303
end: 1314
orientation: inline

BBa_J36801
type: SequenceAnnotation
persistent identity: annotation1909055
display id: annotation1909055
version: 1
top level: KaiA_KaiC_
location: https://synbiohub.org/public/igem/BBa_J36335/annotation1909055/range1909055/1
component: BBa_J36801

https://synbiohub.org/public/igem/BBa_J36335/annotation1909055/range1909055/1
type: Range
persistent identity: range1909055
display id: range1909055
version: 1
direction: direction/reverse
top level: KaiA_KaiC_
start: 229
end: 1086
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1909056
display id: annotation1909056
version: 1
top level: KaiA_KaiC_
location: https://synbiohub.org/public/igem/BBa_J36335/annotation1909056/range1909056/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J36335/annotation1909056/range1909056/1
type: Range
persistent identity: range1909056
display id: range1909056
version: 1
direction: direction/forward
top level: KaiA_KaiC_
start: 1095
end: 1294
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1909046
display id: annotation1909046
version: 1
top level: KaiA_KaiC_
location: https://synbiohub.org/public/igem/BBa_J36335/annotation1909046/range1909046/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J36335/annotation1909046/range1909046/1
type: Range
persistent identity: range1909046
display id: range1909046
version: 1
direction: direction/forward
top level: KaiA_KaiC_
start: 1
end: 200
orientation: inline

BBa_J36804
type: SequenceAnnotation
persistent identity: annotation1909065
display id: annotation1909065
version: 1
top level: KaiA_KaiC_
location: https://synbiohub.org/public/igem/BBa_J36335/annotation1909065/range1909065/1
component: BBa_J36804

https://synbiohub.org/public/igem/BBa_J36335/annotation1909065/range1909065/1
type: Range
persistent identity: range1909065
display id: range1909065
version: 1
direction: direction/reverse
top level: KaiA_KaiC_
start: 1321
end: 2883
orientation: inline

BBa_I761014
type: ComponentDefinition
persistent identity: BBa I761014
display id: BBa_I761014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cinr+cinl (RBS) with double terminator
creator: Chih-Hsien Yang
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
group u list: _140_
m user id: 0
owner id: 592
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The RBS (BBa_B0034) was combined with cinr (BBa_C0077), cinl (BBa_C0076) and D-term (BBa_B0014) to create a single transcription control device for BBa_R0078
mutable notes: Comparing to BBa_I761000, double terminator is added in the end of this construct
mutable provenance: BioBrick
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0014
component: BBa_I761000
sequence annotation: BBa_B0014
sequence annotation: BBa_I761000
sequence: https://synbiohub.org/public/igem/BBa_I761014_sequence/1

BBa_B0014
type: Component
persistent identity: component2261227
display id: component2261227
version: 1
top level: BBa_I761014
access: public
definition: https://synbiohub.org/public/igem/BBa_B0014/1

BBa_I761000
type: Component
persistent identity: component2261220
display id: component2261220
version: 1
top level: BBa_I761014
access: public
definition: https://synbiohub.org/public/igem/BBa_I761000/1

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2261227
display id: annotation2261227
version: 1
top level: BBa_I761014
location: https://synbiohub.org/public/igem/BBa_I761014/annotation2261227/range2261227/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_I761014/annotation2261227/range2261227/1
type: Range
persistent identity: range2261227
display id: range2261227
version: 1
direction: direction/reverse
top level: BBa_I761014
start: 1567
end: 1661
orientation: inline

BBa_I761000
type: SequenceAnnotation
persistent identity: annotation2261220
display id: annotation2261220
version: 1
top level: BBa_I761014
location: https://synbiohub.org/public/igem/BBa_I761014/annotation2261220/range2261220/1
component: BBa_I761000

https://synbiohub.org/public/igem/BBa_I761014/annotation2261220/range2261220/1
type: Range
persistent identity: range2261220
display id: range2261220
version: 1
direction: direction/reverse
top level: BBa_I761014
start: 1
end: 1558
orientation: inline

BBa_K1150043
type: ComponentDefinition
persistent identity: BBa K1150043
display id: BBa_K1150043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: uniCAS RNAimer multiple targets to VEGF 2 and VEGF 3
creator: Ilona Blank
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1150038
component: BBa_K1150037
sequence annotation: BBa_K1150038
sequence annotation: BBa_K1150037
sequence: https://synbiohub.org/public/igem/BBa_K1150043_sequence/1

BBa_K1150038
type: Component
persistent identity: component2353545
display id: component2353545
version: 1
top level: BBa_K1150043
access: public
definition: BBa_K1150038

BBa_K1150037
type: Component
persistent identity: component2353544
display id: component2353544
version: 1
top level: BBa_K1150043
access: public
definition: BBa_K1150037

BBa_K1150038
type: SequenceAnnotation
persistent identity: annotation2353545
display id: annotation2353545
version: 1
top level: BBa_K1150043
location: https://synbiohub.org/public/igem/BBa_K1150043/annotation2353545/range2353545/1
component: BBa_K1150038

https://synbiohub.org/public/igem/BBa_K1150043/annotation2353545/range2353545/1
type: Range
persistent identity: range2353545
display id: range2353545
version: 1
direction: direction/reverse
top level: BBa_K1150043
start: 838
end: 1666
orientation: inline

BBa_K1150037
type: SequenceAnnotation
persistent identity: annotation2353544
display id: annotation2353544
version: 1
top level: BBa_K1150043
location: https://synbiohub.org/public/igem/BBa_K1150043/annotation2353544/range2353544/1
component: BBa_K1150037

https://synbiohub.org/public/igem/BBa_K1150043/annotation2353544/range2353544/1
type: Range
persistent identity: range2353544
display id: range2353544
version: 1
direction: direction/reverse
top level: BBa_K1150043
start: 1
end: 829
orientation: inline

BBa_I759018
type: ComponentDefinition
persistent identity: BBa I759018
display id: BBa_I759018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cis6-repressed, tet-regulated YFP
creator: Kat Pak
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: true
experience: experience/None
group u list: _115_
m user id: 0
owner id: 1834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has been shown to repress the expression of YFP via a cis-repressive element called cr6 which sequesters the ribosome binding site of YFP
mutable provenance: The cr element is a synthetic oligo which has been inserted into part I759009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_S03724
component: BBa_I759042
sequence annotation: BBa_I759042
sequence annotation: BBa_S03724
sequence: https://synbiohub.org/public/igem/BBa_I759018_sequence/1

BBa_S03724
type: Component
persistent identity: component2271226
display id: component2271226
version: 1
top level: BBa_I759018
access: public
definition: https://synbiohub.org/public/igem/BBa_S03724/1

BBa_I759042
type: Component
persistent identity: component2271203
display id: component2271203
version: 1
top level: BBa_I759018
access: public
definition: https://synbiohub.org/public/igem/BBa_I759042/1

BBa_I759042
type: SequenceAnnotation
persistent identity: annotation2271203
display id: annotation2271203
version: 1
top level: BBa_I759018
location: https://synbiohub.org/public/igem/BBa_I759018/annotation2271203/range2271203/1
component: BBa_I759042

https://synbiohub.org/public/igem/BBa_I759018/annotation2271203/range2271203/1
type: Range
persistent identity: range2271203
display id: range2271203
version: 1
direction: direction/reverse
top level: BBa_I759018
start: 1
end: 833
orientation: inline

BBa_S03724
type: SequenceAnnotation
persistent identity: annotation2271226
display id: annotation2271226
version: 1
top level: BBa_I759018
location: https://synbiohub.org/public/igem/BBa_I759018/annotation2271226/range2271226/1
component: BBa_S03724

https://synbiohub.org/public/igem/BBa_I759018/annotation2271226/range2271226/1
type: Range
persistent identity: range2271226
display id: range2271226
version: 1
direction: direction/reverse
top level: BBa_I759018
start: 842
end: 1861
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C2 OmpR
sequence annotation: -10
sequence annotation: C3 OmpR
sequence annotation: C1 OmpR
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
direction: direction/forward
top level: OmpR
start: 34
end: 51
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
direction: direction/forward
top level: OmpR
start: 98
end: 103
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
direction: direction/forward
top level: OmpR
start: 54
end: 71
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
direction: direction/forward
top level: OmpR
start: 75
end: 80
orientation: inline

BBa_J23113
type: ComponentDefinition
persistent identity: BBa J23113
display id: BBa_J23113
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23113_sequence/1

ecfp
type: ComponentDefinition
persistent identity: BBa I15016
display id: BBa_I15016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.ECFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to produce ECFP under the medium RBS B0032
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0020
component: BBa_B0032
sequence annotation: BBa_E0020
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I15016_sequence/1

BBa_E0020
type: Component
persistent identity: component1478408
display id: component1478408
version: 1
top level: ecfp
access: public
definition: ecfp

BBa_B0032
type: Component
persistent identity: component1478405
display id: component1478405
version: 1
top level: ecfp
access: public
definition: BBa_B0032

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1478408
display id: annotation1478408
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15016/annotation1478408/range1478408/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I15016/annotation1478408/range1478408/1
type: Range
persistent identity: range1478408
display id: range1478408
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 742
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478405
display id: annotation1478405
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15016/annotation1478405/range1478405/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15016/annotation1478405/range1478405/1
type: Range
persistent identity: range1478405
display id: range1478405
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 13
orientation: inline

BBa_I759003
type: ComponentDefinition
persistent identity: BBa I759003
display id: BBa_I759003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tet-regulated Q (Weak tetR promoter)
creator: Josh Michener
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _115_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pTet-spacer-Q-term-J23116-tetR-term
mutable notes: None
mutable provenance: Caltech iGEM
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I759000
component: BBa_S03724
sequence annotation: BBa_S03724
sequence annotation: BBa_I759000
sequence: https://synbiohub.org/public/igem/BBa_I759003_sequence/1

BBa_I759000
type: Component
persistent identity: component2270554
display id: component2270554
version: 1
top level: BBa_I759003
access: public
definition: https://synbiohub.org/public/igem/BBa_I759000/1

BBa_S03724
type: Component
persistent identity: component2270579
display id: component2270579
version: 1
top level: BBa_I759003
access: public
definition: https://synbiohub.org/public/igem/BBa_S03724/1

BBa_S03724
type: SequenceAnnotation
persistent identity: annotation2270579
display id: annotation2270579
version: 1
top level: BBa_I759003
location: https://synbiohub.org/public/igem/BBa_I759003/annotation2270579/range2270579/1
component: BBa_S03724

https://synbiohub.org/public/igem/BBa_I759003/annotation2270579/range2270579/1
type: Range
persistent identity: range2270579
display id: range2270579
version: 1
direction: direction/reverse
top level: BBa_I759003
start: 744
end: 1763
orientation: inline

BBa_I759000
type: SequenceAnnotation
persistent identity: annotation2270554
display id: annotation2270554
version: 1
top level: BBa_I759003
location: https://synbiohub.org/public/igem/BBa_I759003/annotation2270554/range2270554/1
component: BBa_I759000

https://synbiohub.org/public/igem/BBa_I759003/annotation2270554/range2270554/1
type: Range
persistent identity: range2270554
display id: range2270554
version: 1
direction: direction/reverse
top level: BBa_I759003
start: 1
end: 735
orientation: inline

SbpA | RFP
type: ComponentDefinition
persistent identity: BBa K525404
display id: BBa_K525404
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Fusion Protein of S-Layer SbpA and mCherry RFP
creator: Timo Wolf
created: 2011-09-09T11:00:00Z
modified: 2016-01-26T09:41:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _690_
m user id: 4206
owner id: 6668
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Fusion Protein of S-Layer sbpA and mCherry RFP
mutable notes: Fusion Protein of BBa_K525403 and BBa_J18932
mutable provenance: Fusion Protein of S-Layer sbpA and mCherry RFP
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: K525401
sequence annotation: lac operator
sequence annotation: RBS
sequence annotation: J18932
sequence annotation: B0034
sequence annotation: SbpA domain
sequence annotation: ATG
sequence annotation: Freiburg scar
sequence annotation: NgoMIV
sequence annotation: AgeI
sequence annotation: TAA
sequence annotation: T7 promoter
sequence annotation: mCherry (RFP)
sequence: https://synbiohub.org/public/igem/BBa_K525404_sequence/1

K525401
type: SequenceAnnotation
persistent identity: annotation2129908
display id: annotation2129908
version: 1
top level: SbpA | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129908/range2129908/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129908/range2129908/1
type: Range
persistent identity: range2129908
display id: range2129908
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 73
end: 3192
orientation: inline

lac operator
type: SequenceAnnotation
persistent identity: annotation2128865
display id: annotation2128865
version: 1
top level: SbpA | RFP
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2128865/range2128865/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2128865/range2128865/1
type: Range
persistent identity: range2128865
display id: range2128865
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 20
end: 44
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2129906
display id: annotation2129906
version: 1
top level: SbpA | RFP
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129906/range2129906/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129906/range2129906/1
type: Range
persistent identity: range2129906
display id: range2129906
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 56
end: 59
orientation: inline

J18932
type: SequenceAnnotation
persistent identity: annotation2129914
display id: annotation2129914
version: 1
top level: SbpA | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129914/range2129914/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129914/range2129914/1
type: Range
persistent identity: range2129914
display id: range2129914
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3199
end: 3912
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation2129905
display id: annotation2129905
version: 1
top level: SbpA | RFP
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129905/range2129905/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129905/range2129905/1
type: Range
persistent identity: range2129905
display id: range2129905
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 53
end: 64
orientation: inline

SbpA domain
type: SequenceAnnotation
persistent identity: annotation2129913
display id: annotation2129913
version: 1
top level: SbpA | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129913/range2129913/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129913/range2129913/1
type: Range
persistent identity: range2129913
display id: range2129913
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 73
end: 3192
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2129907
display id: annotation2129907
version: 1
top level: SbpA | RFP
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129907/range2129907/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129907/range2129907/1
type: Range
persistent identity: range2129907
display id: range2129907
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 73
end: 75
orientation: inline

Freiburg scar
type: SequenceAnnotation
persistent identity: annotation2129912
display id: annotation2129912
version: 1
top level: SbpA | RFP
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129912/range2129912/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129912/range2129912/1
type: Range
persistent identity: range2129912
display id: range2129912
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3193
end: 3198
orientation: inline

NgoMIV
type: SequenceAnnotation
persistent identity: annotation2129909
display id: annotation2129909
version: 1
top level: SbpA | RFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129909/range2129909/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129909/range2129909/1
type: Range
persistent identity: range2129909
display id: range2129909
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 76
end: 81
orientation: inline

AgeI
type: SequenceAnnotation
persistent identity: annotation2129910
display id: annotation2129910
version: 1
top level: SbpA | RFP
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129910/range2129910/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129910/range2129910/1
type: Range
persistent identity: range2129910
display id: range2129910
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3904
end: 3909
orientation: inline

TAA
type: SequenceAnnotation
persistent identity: annotation2129911
display id: annotation2129911
version: 1
top level: SbpA | RFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129911/range2129911/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129911/range2129911/1
type: Range
persistent identity: range2129911
display id: range2129911
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3910
end: 3912
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation2128864
display id: annotation2128864
version: 1
top level: SbpA | RFP
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2128864/range2128864/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2128864/range2128864/1
type: Range
persistent identity: range2128864
display id: range2128864
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 1
end: 20
orientation: inline

mCherry (RFP)
type: SequenceAnnotation
persistent identity: annotation2129915
display id: annotation2129915
version: 1
top level: SbpA | RFP
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K525404/annotation2129915/range2129915/1

https://synbiohub.org/public/igem/BBa_K525404/annotation2129915/range2129915/1
type: Range
persistent identity: range2129915
display id: range2129915
version: 1
direction: direction/forward
top level: SbpA | RFP
start: 3199
end: 3912
orientation: inline

BBa_I751101
type: ComponentDefinition
persistent identity: BBa I751101
display id: BBa_I751101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: J540140dPr+hybrid promoter(plux-lac)
creator: Kenichiro Iwasaki
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:06Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A4HPlac
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: plux-lac hybrid promoter
sequence annotation: SalI
sequence annotation: R0063
sequence annotation: B0034
sequence annotation: C0062
sequence annotation: B0010
sequence annotation: J54033
sequence annotation: J54025
sequence annotation: E0040
sequence annotation: BglII
sequence annotation: B0012
sequence annotation: BamHI
sequence annotation: MluI
sequence annotation: B0030
sequence: https://synbiohub.org/public/igem/BBa_I751101_sequence/1

plux-lac hybrid promoter
type: SequenceAnnotation
persistent identity: annotation1954400
display id: annotation1954400
version: 1
top level: BBa_I751101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954400/range1954400/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954400/range1954400/1
type: Range
persistent identity: range1954400
display id: range1954400
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1122
end: 1195
orientation: inline

SalI
type: SequenceAnnotation
persistent identity: annotation1954824
display id: annotation1954824
version: 1
top level: BBa_I751101
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954824/range1954824/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954824/range1954824/1
type: Range
persistent identity: range1954824
display id: range1954824
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1104
end: 1109
orientation: inline

R0063
type: SequenceAnnotation
persistent identity: annotation1954818
display id: annotation1954818
version: 1
top level: BBa_I751101
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954818/range1954818/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954818/range1954818/1
type: Range
persistent identity: range1954818
display id: range1954818
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1
end: 151
orientation: inline

B0034
type: SequenceAnnotation
persistent identity: annotation1954819
display id: annotation1954819
version: 1
top level: BBa_I751101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954819/range1954819/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954819/range1954819/1
type: Range
persistent identity: range1954819
display id: range1954819
version: 1
direction: direction/forward
top level: BBa_I751101
start: 160
end: 171
orientation: inline

C0062
type: SequenceAnnotation
persistent identity: annotation1954820
display id: annotation1954820
version: 1
top level: BBa_I751101
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954820/range1954820/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954820/range1954820/1
type: Range
persistent identity: range1954820
display id: range1954820
version: 1
direction: direction/forward
top level: BBa_I751101
start: 178
end: 958
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation1954831
display id: annotation1954831
version: 1
top level: BBa_I751101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954831/range1954831/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954831/range1954831/1
type: Range
persistent identity: range1954831
display id: range1954831
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1985
end: 2063
orientation: inline

J54033
type: SequenceAnnotation
persistent identity: annotation1954823
display id: annotation1954823
version: 1
top level: BBa_I751101
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954823/range1954823/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954823/range1954823/1
type: Range
persistent identity: range1954823
display id: range1954823
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1104
end: 1123
orientation: inline

J54025
type: SequenceAnnotation
persistent identity: annotation1954828
display id: annotation1954828
version: 1
top level: BBa_I751101
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954828/range1954828/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954828/range1954828/1
type: Range
persistent identity: range1954828
display id: range1954828
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1208
end: 1227
orientation: inline

E0040
type: SequenceAnnotation
persistent identity: annotation1954830
display id: annotation1954830
version: 1
top level: BBa_I751101
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954830/range1954830/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954830/range1954830/1
type: Range
persistent identity: range1954830
display id: range1954830
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1257
end: 1975
orientation: inline

B0010
type: SequenceAnnotation
persistent identity: annotation1954821
display id: annotation1954821
version: 1
top level: BBa_I751101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954821/range1954821/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954821/range1954821/1
type: Range
persistent identity: range1954821
display id: range1954821
version: 1
direction: direction/forward
top level: BBa_I751101
start: 967
end: 1046
orientation: inline

BglII
type: SequenceAnnotation
persistent identity: annotation1954826
display id: annotation1954826
version: 1
top level: BBa_I751101
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954826/range1954826/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954826/range1954826/1
type: Range
persistent identity: range1954826
display id: range1954826
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1208
end: 1213
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation1954822
display id: annotation1954822
version: 1
top level: BBa_I751101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954822/range1954822/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954822/range1954822/1
type: Range
persistent identity: range1954822
display id: range1954822
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1055
end: 1095
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1954825
display id: annotation1954825
version: 1
top level: BBa_I751101
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954825/range1954825/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954825/range1954825/1
type: Range
persistent identity: range1954825
display id: range1954825
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1118
end: 1123
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation1954832
display id: annotation1954832
version: 1
top level: BBa_I751101
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954832/range1954832/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954832/range1954832/1
type: Range
persistent identity: range1954832
display id: range1954832
version: 1
direction: direction/forward
top level: BBa_I751101
start: 2073
end: 2113
orientation: inline

MluI
type: SequenceAnnotation
persistent identity: annotation1954827
display id: annotation1954827
version: 1
top level: BBa_I751101
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954827/range1954827/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954827/range1954827/1
type: Range
persistent identity: range1954827
display id: range1954827
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1222
end: 1227
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation1954829
display id: annotation1954829
version: 1
top level: BBa_I751101
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_I751101/annotation1954829/range1954829/1

https://synbiohub.org/public/igem/BBa_I751101/annotation1954829/range1954829/1
type: Range
persistent identity: range1954829
display id: range1954829
version: 1
direction: direction/forward
top level: BBa_I751101
start: 1236
end: 1250
orientation: inline

ATF1 gener
type: ComponentDefinition
persistent identity: BBa J45199
display id: BBa_J45199
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor enzyme (ATF1) generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is an intermediate to the protein generator BBa_45200.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0030
component: BBa_J45014
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_J45014
sequence: https://synbiohub.org/public/igem/BBa_J45199_sequence/1

BBa_B0010
type: Component
persistent identity: component1908016
display id: component1908016
version: 1
top level: ATF1 gener
access: public
definition: BBa_B0010

BBa_B0030
type: Component
persistent identity: component1908009
display id: component1908009
version: 1
top level: ATF1 gener
access: public
definition: BBa_B0030

BBa_J45014
type: Component
persistent identity: component1908015
display id: component1908015
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_J45014/1

BBa_B0012
type: Component
persistent identity: component1908018
display id: component1908018
version: 1
top level: ATF1 gener
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908016
display id: annotation1908016
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908016/range1908016/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45199/annotation1908016/range1908016/1
type: Range
persistent identity: range1908016
display id: range1908016
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1611
end: 1690
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908018
display id: annotation1908018
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908018/range1908018/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45199/annotation1908018/range1908018/1
type: Range
persistent identity: range1908018
display id: range1908018
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1699
end: 1739
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1908009
display id: annotation1908009
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908009/range1908009/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45199/annotation1908009/range1908009/1
type: Range
persistent identity: range1908009
display id: range1908009
version: 1
direction: direction/forward
top level: ATF1 gener
start: 1
end: 15
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908015
display id: annotation1908015
version: 1
top level: ATF1 gener
location: https://synbiohub.org/public/igem/BBa_J45199/annotation1908015/range1908015/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45199/annotation1908015/range1908015/1
type: Range
persistent identity: range1908015
display id: range1908015
version: 1
direction: direction/reverse
top level: ATF1 gener
start: 22
end: 1602
orientation: inline

fwYellow
type: ComponentDefinition
persistent identity: BBa K1033910
display id: BBa_K1033910
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: fwYellow, yellow chromoprotein
creator: Sabri Jamal
created: 2013-09-16T11:00:00Z
modified: 2016-01-25T02:41:53Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1340_
m user id: 4206
owner id: 13997
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein naturally exhibits strong color when expressed.
mutable notes: This construct is synthetic. It was synthesized from a template where the consensus sequence for the "color dependancy" in order to obtain a chromoprotein with a unique yellow color.
mutable provenance: Synthetic chromoprotein from DNA 2.0.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: fwYellow
sequence: https://synbiohub.org/public/igem/BBa_K1033910_sequence/1

fwYellow
type: SequenceAnnotation
persistent identity: annotation2371218
display id: annotation2371218
version: 1
top level: fwYellow
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1033910/annotation2371218/range2371218/1

https://synbiohub.org/public/igem/BBa_K1033910/annotation2371218/range2371218/1
type: Range
persistent identity: range2371218
display id: range2371218
version: 1
direction: direction/forward
top level: fwYellow
start: 1
end: 711
orientation: inline

BBa_P0440
type: ComponentDefinition
persistent identity: BBa P0440
display id: BBa_P0440
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> TetR [S0151]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_C0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_P0440_sequence/1

BBa_B0012
type: Component
persistent identity: component944912
display id: component944912
version: 1
top level: BBa_P0440
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component944886
display id: component944886
version: 1
top level: BBa_P0440
access: public
definition: BBa_B0034

BBa_B0010
type: Component
persistent identity: component944902
display id: component944902
version: 1
top level: BBa_P0440
access: public
definition: BBa_B0010

BBa_C0040
type: Component
persistent identity: component944896
display id: component944896
version: 1
top level: BBa_P0440
access: public
definition: tetR

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944912
display id: annotation944912
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
type: Range
persistent identity: range944912
display id: range944912
version: 1
direction: direction/forward
top level: BBa_P0440
start: 800
end: 840
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944902
display id: annotation944902
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
type: Range
persistent identity: range944902
display id: range944902
version: 1
direction: direction/forward
top level: BBa_P0440
start: 712
end: 791
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944886
display id: annotation944886
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
type: Range
persistent identity: range944886
display id: range944886
version: 1
direction: direction/forward
top level: BBa_P0440
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944896
display id: annotation944896
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
type: Range
persistent identity: range944896
display id: range944896
version: 1
direction: direction/forward
top level: BBa_P0440
start: 19
end: 678
orientation: inline

BBa_K137018
type: ComponentDefinition
persistent identity: BBa K137018
display id: BBa_K137018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AHL receiver + GFP
creator: Doug Tischer
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:10:08Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3049
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This parts produces GFP in response to detecting an acylhomoserinelactone (AHL). It was made to check that the RBS behind the luxR reciever was functional.
mutable notes: None
mutable provenance: All registry parts.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_F2622
component: BBa_I13401
component: BBa_B0034
sequence annotation: BBa_I13401
sequence annotation: BBa_B0034
sequence annotation: BBa_F2622
sequence: https://synbiohub.org/public/igem/BBa_K137018_sequence/1

BBa_F2622
type: Component
persistent identity: component2281291
display id: component2281291
version: 1
top level: BBa_K137018
access: public
definition: BBa_F2622

BBa_I13401
type: Component
persistent identity: component2281301
display id: component2281301
version: 1
top level: BBa_K137018
access: public
definition: BBa_I13401

BBa_B0034
type: Component
persistent identity: component2281293
display id: component2281293
version: 1
top level: BBa_K137018
access: public
definition: BBa_B0034

BBa_I13401
type: SequenceAnnotation
persistent identity: annotation2281301
display id: annotation2281301
version: 1
top level: BBa_K137018
location: https://synbiohub.org/public/igem/BBa_K137018/annotation2281301/range2281301/1
component: BBa_I13401

https://synbiohub.org/public/igem/BBa_K137018/annotation2281301/range2281301/1
type: Range
persistent identity: range2281301
display id: range2281301
version: 1
direction: direction/reverse
top level: BBa_K137018
start: 1089
end: 1945
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2281293
display id: annotation2281293
version: 1
top level: BBa_K137018
location: https://synbiohub.org/public/igem/BBa_K137018/annotation2281293/range2281293/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137018/annotation2281293/range2281293/1
type: Range
persistent identity: range2281293
display id: range2281293
version: 1
direction: direction/reverse
top level: BBa_K137018
start: 1071
end: 1082
orientation: inline

BBa_F2622
type: SequenceAnnotation
persistent identity: annotation2281291
display id: annotation2281291
version: 1
top level: BBa_K137018
location: https://synbiohub.org/public/igem/BBa_K137018/annotation2281291/range2281291/1
component: BBa_F2622

https://synbiohub.org/public/igem/BBa_K137018/annotation2281291/range2281291/1
type: Range
persistent identity: range2281291
display id: range2281291
version: 1
direction: direction/reverse
top level: BBa_K137018
start: 1
end: 1062
orientation: inline

BBa_I746200
type: ComponentDefinition
persistent identity: BBa I746200
display id: BBa_I746200
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.
creator: Narin Hengrung
created: 2007-08-28T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2118
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted.
mutable notes: The original mutants had an EcoR1 restriction site within their coding sequence. This had to be removed via site directed mutagenesis, using a megaprimer based method.
mutable provenance: The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I746200_sequence/1

rbs+LIMS1
type: ComponentDefinition
persistent identity: BBa I742111
display id: BBa_I742111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Limonene synthase gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Ribosome binding site attached to Limonene synthase gene. Limonene synthase enzyme produces (+)-limonene from farnesyl diphosphate (intermediate in mevlonate pathway). (+) Limonene is a component of lemon scent.
mutable notes: several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG. LIMS cDNA was used as genomic DNA contains introns.
mutable provenance: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases, J Lucker, et al, Eur. J. Biochem. 269: 3160???3171 (2002) Analysis of Lemon and Bergamot Essential Oils by HPLC with Microbore Columns, M Benincasa, et al, Chromatographia 30(5/6):271 (1990) Citrus fruit flavour and aroma biosynthesis, L Sharon-Asa, The Plant Journal, 36:664 (2003)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_I742110
sequence annotation: BBa_J15001
sequence annotation: BBa_I742110
sequence: https://synbiohub.org/public/igem/BBa_I742111_sequence/1

BBa_J15001
type: Component
persistent identity: component1955370
display id: component1955370
version: 1
top level: rbs+LIMS1
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I742110
type: Component
persistent identity: component1955374
display id: component1955374
version: 1
top level: rbs+LIMS1
access: public
definition: https://synbiohub.org/public/igem/BBa_I742110/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1955370
display id: annotation1955370
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742111/annotation1955370/range1955370/1
type: Range
persistent identity: range1955370
display id: range1955370
version: 1
direction: direction/reverse
top level: rbs+LIMS1
start: 1
end: 10
orientation: inline

BBa_I742110
type: SequenceAnnotation
persistent identity: annotation1955374
display id: annotation1955374
version: 1
top level: rbs+LIMS1
location: https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
component: BBa_I742110

https://synbiohub.org/public/igem/BBa_I742111/annotation1955374/range1955374/1
type: Range
persistent identity: range1955374
display id: range1955374
version: 1
direction: direction/reverse
top level: rbs+LIMS1
start: 17
end: 1687
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

ecfp
type: ComponentDefinition
persistent identity: BBa I15017
display id: BBa_I15017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.EYFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to generate EYFP under the medium strength RBS B0032.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_E0030
sequence annotation: BBa_B0032
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I15017_sequence/1

BBa_B0032
type: Component
persistent identity: component1478419
display id: component1478419
version: 1
top level: ecfp
access: public
definition: BBa_B0032

BBa_E0030
type: Component
persistent identity: component1478422
display id: component1478422
version: 1
top level: ecfp
access: public
definition: https://synbiohub.org/public/igem/BBa_E0030/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478419
display id: annotation1478419
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15017/annotation1478419/range1478419/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15017/annotation1478419/range1478419/1
type: Range
persistent identity: range1478419
display id: range1478419
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 13
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1478422
display id: annotation1478422
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15017/annotation1478422/range1478422/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I15017/annotation1478422/range1478422/1
type: Range
persistent identity: range1478422
display id: range1478422
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 742
orientation: inline

BBa_E0422
type: ComponentDefinition
persistent identity: BBa E0422
display id: BBa_E0422
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E0022
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_E0422_sequence/1

BBa_B0010
type: Component
persistent identity: component942823
display id: component942823
version: 1
top level: BBa_E0422
access: public
definition: BBa_B0010

BBa_E0022
type: Component
persistent identity: component942815
display id: component942815
version: 1
top level: BBa_E0422
access: public
definition: ECFP

BBa_B0034
type: Component
persistent identity: component942803
display id: component942803
version: 1
top level: BBa_E0422
access: public
definition: BBa_B0034

BBa_B0012
type: Component
persistent identity: component942833
display id: component942833
version: 1
top level: BBa_E0422
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942833
display id: annotation942833
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0422/annotation942833/range942833/1
type: Range
persistent identity: range942833
display id: range942833
version: 1
direction: direction/forward
top level: BBa_E0422
start: 877
end: 917
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942823
display id: annotation942823
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0422/annotation942823/range942823/1
type: Range
persistent identity: range942823
display id: range942823
version: 1
direction: direction/forward
top level: BBa_E0422
start: 789
end: 868
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942803
display id: annotation942803
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0422/annotation942803/range942803/1
type: Range
persistent identity: range942803
display id: range942803
version: 1
direction: direction/forward
top level: BBa_E0422
start: 1
end: 12
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation942815
display id: annotation942815
version: 1
top level: BBa_E0422
location: https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_E0422/annotation942815/range942815/1
type: Range
persistent identity: range942815
display id: range942815
version: 1
direction: direction/forward
top level: BBa_E0422
start: 19
end: 780
orientation: inline

BBa_K118004
type: ComponentDefinition
persistent identity: BBa K118004
display id: BBa_K118004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+dxs (1-deoxyxylulose-5-phosphate synthase)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of dxs from Escherichia coli JM109 with added ribosome binding site (rbs). It encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses the first step in the biosynthesis of terpenoids. Overexpression of dxs has been reported to increase yields of carotenoids and other terpenoids [add reference here].
mutable notes: No special considerations.
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_K118000
sequence annotation: BBa_J15001
sequence annotation: BBa_K118000
sequence: https://synbiohub.org/public/igem/BBa_K118004_sequence/1

BBa_J15001
type: Component
persistent identity: component1970353
display id: component1970353
version: 1
top level: BBa_K118004
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118000
type: Component
persistent identity: component1970354
display id: component1970354
version: 1
top level: BBa_K118004
access: public
definition: dxs

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970353
display id: annotation1970353
version: 1
top level: BBa_K118004
location: https://synbiohub.org/public/igem/BBa_K118004/annotation1970353/range1970353/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118004/annotation1970353/range1970353/1
type: Range
persistent identity: range1970353
display id: range1970353
version: 1
direction: direction/reverse
top level: BBa_K118004
start: 1
end: 10
orientation: inline

BBa_K118000
type: SequenceAnnotation
persistent identity: annotation1970354
display id: annotation1970354
version: 1
top level: BBa_K118004
location: https://synbiohub.org/public/igem/BBa_K118004/annotation1970354/range1970354/1
component: BBa_K118000

https://synbiohub.org/public/igem/BBa_K118004/annotation1970354/range1970354/1
type: Range
persistent identity: range1970354
display id: range1970354
version: 1
direction: direction/reverse
top level: BBa_K118004
start: 17
end: 1882
orientation: inline

BBa_I13452
type: ComponentDefinition
persistent identity: BBa I13452
display id: BBa_I13452
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Adds another BioBrick site
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains both a BbsI and a BstXI restriction site. When cut with both enzymes, it leaves an EcoRI and a PstI site.
mutable notes: 70 bp from Eco to Spe or Xba to Pst, to allow for gel extraction. The middle portion is largely noise, intended as filler to raise the size of the part.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BbsI site
sequence annotation: Future Eco site
sequence annotation: Future Pst site
sequence annotation: BstXI site
sequence: https://synbiohub.org/public/igem/BBa_I13452_sequence/1

BbsI site
type: SequenceAnnotation
persistent identity: annotation1891620
display id: annotation1891620
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891620/range1891620/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891620/range1891620/1
type: Range
persistent identity: range1891620
display id: range1891620
version: 1
direction: direction/forward
top level: BBa_I13452
start: 8
end: 13
orientation: inline

Future Eco site
type: SequenceAnnotation
persistent identity: annotation1891619
display id: annotation1891619
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891619/range1891619/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891619/range1891619/1
type: Range
persistent identity: range1891619
display id: range1891619
version: 1
direction: direction/forward
top level: BBa_I13452
start: 1
end: 6
orientation: inline

Future Pst site
type: SequenceAnnotation
persistent identity: annotation1891622
display id: annotation1891622
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891622/range1891622/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891622/range1891622/1
type: Range
persistent identity: range1891622
display id: range1891622
version: 1
direction: direction/forward
top level: BBa_I13452
start: 44
end: 49
orientation: inline

BstXI site
type: SequenceAnnotation
persistent identity: annotation1891621
display id: annotation1891621
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891621/range1891621/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891621/range1891621/1
type: Range
persistent identity: range1891621
display id: range1891621
version: 1
direction: direction/forward
top level: BBa_I13452
start: 41
end: 52
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0026
display id: BBa_E0026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0026_sequence/1

BBa_K1061001
type: ComponentDefinition
persistent identity: BBa K1061001
display id: BBa_K1061001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: apoptin
creator: mengyi sun
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1369_
m user id: 0
owner id: 11892
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Apoptin is a protein that is originally isolated from the chicken anemia virus(CAV). It has been found that expressing this gene can induce apoptosis in a broad spectrum of cancer cells but not in normal cells. Hence it is a very powerful tool in treating cancer. Fusion the TAT protein with apoptin can even achieve by-stander effect, and we try to use it in the most simple device of our project. We have successfully expressed it in Hep G2 cell lines and observe a brilliant apoptosis effect.
mutable notes: Although it has been try in a broad spectrum of cells and prove to be safe to the normal cells, it still has not been tried in ALL normal cells. So before using it to treat certain kinds of cancer, every team need to ensure that it want hurt the specify normal cells involve in the project. And it is a protein contain 121 amino acid that may big enough to induce immune reaction(although in the experiment of mouse it has not been reported.)
mutable provenance: Cloning from plasmid from Zhuang's lab.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: leucine rich fragment
sequence annotation: NLS 1
sequence: https://synbiohub.org/public/igem/BBa_K1061001_sequence/1

leucine rich fragment
type: SequenceAnnotation
persistent identity: annotation2339023
display id: annotation2339023
version: 1
top level: BBa_K1061001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1061001/annotation2339023/range2339023/1

https://synbiohub.org/public/igem/BBa_K1061001/annotation2339023/range2339023/1
type: Range
persistent identity: range2339023
display id: range2339023
version: 1
direction: direction/forward
top level: BBa_K1061001
start: 97
end: 138
orientation: inline

NLS 1
type: SequenceAnnotation
persistent identity: annotation2362082
display id: annotation2362082
version: 1
top level: BBa_K1061001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_K1061001/annotation2362082/range2362082/1

https://synbiohub.org/public/igem/BBa_K1061001/annotation2362082/range2362082/1
type: Range
persistent identity: range2362082
display id: range2362082
version: 1
direction: direction/forward
top level: BBa_K1061001
start: 244
end: 264
orientation: inline

BBa_I759016
type: ComponentDefinition
persistent identity: BBa I759016
display id: BBa_I759016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cis4-repressed, tet-regulated YFP
creator: Kat Pak
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _115_
m user id: 0
owner id: 1834
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part has been shown to repress the expression of YFP via a cis-repressive element called cr4 which sequesters the ribosome binding site of YFP.
mutable provenance: The cr element is a synthetic oligo which has been inserted into part I759009.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I759038
component: BBa_S03724
sequence annotation: BBa_S03724
sequence annotation: BBa_I759038
sequence: https://synbiohub.org/public/igem/BBa_I759016_sequence/1

BBa_I759038
type: Component
persistent identity: component2271149
display id: component2271149
version: 1
top level: BBa_I759016
access: public
definition: https://synbiohub.org/public/igem/BBa_I759038/1

BBa_S03724
type: Component
persistent identity: component2271172
display id: component2271172
version: 1
top level: BBa_I759016
access: public
definition: https://synbiohub.org/public/igem/BBa_S03724/1

BBa_S03724
type: SequenceAnnotation
persistent identity: annotation2271172
display id: annotation2271172
version: 1
top level: BBa_I759016
location: https://synbiohub.org/public/igem/BBa_I759016/annotation2271172/range2271172/1
component: BBa_S03724

https://synbiohub.org/public/igem/BBa_I759016/annotation2271172/range2271172/1
type: Range
persistent identity: range2271172
display id: range2271172
version: 1
direction: direction/reverse
top level: BBa_I759016
start: 838
end: 1857
orientation: inline

BBa_I759038
type: SequenceAnnotation
persistent identity: annotation2271149
display id: annotation2271149
version: 1
top level: BBa_I759016
location: https://synbiohub.org/public/igem/BBa_I759016/annotation2271149/range2271149/1
component: BBa_I759038

https://synbiohub.org/public/igem/BBa_I759016/annotation2271149/range2271149/1
type: Range
persistent identity: range2271149
display id: range2271149
version: 1
direction: direction/reverse
top level: BBa_I759016
start: 1
end: 829
orientation: inline

BBa_J52011
type: ComponentDefinition
persistent identity: BBa J52011
display id: BBa_J52011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnMyD88-linker-Rluc
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor), which was our main target for modifying the signaling path. dnMyD88 is linked to Rluc (which acts as a reporter) through six aminoacid long protein linker.
mutable notes: dnMyD88-linker-Rluc was made by using PCR ligation technique. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnMyD88-linker-Rluc
sequence annotation: dnMyD88
sequence annotation: linker
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52011_sequence/1

dnMyD88-linker-Rluc
type: SequenceAnnotation
persistent identity: annotation1902846
display id: annotation1902846
version: 1
top level: BBa_J52011
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902846/range1902846/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902846/range1902846/1
type: Range
persistent identity: range1902846
display id: range1902846
version: 1
direction: direction/forward
top level: BBa_J52011
start: 1
end: 1371
orientation: inline

dnMyD88
type: SequenceAnnotation
persistent identity: annotation1902834
display id: annotation1902834
version: 1
top level: BBa_J52011
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902834/range1902834/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902834/range1902834/1
type: Range
persistent identity: range1902834
display id: range1902834
version: 1
direction: direction/forward
top level: BBa_J52011
start: 1
end: 417
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902835
display id: annotation1902835
version: 1
top level: BBa_J52011
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902835/range1902835/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902835/range1902835/1
type: Range
persistent identity: range1902835
display id: range1902835
version: 1
direction: direction/forward
top level: BBa_J52011
start: 418
end: 435
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902836
display id: annotation1902836
version: 1
top level: BBa_J52011
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52011/annotation1902836/range1902836/1

https://synbiohub.org/public/igem/BBa_J52011/annotation1902836/range1902836/1
type: Range
persistent identity: range1902836
display id: range1902836
version: 1
direction: direction/forward
top level: BBa_J52011
start: 436
end: 1371
orientation: inline

BBa_K1150041
type: ComponentDefinition
persistent identity: BBa K1150041
display id: BBa_K1150041
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: uniCAS RNaimer to Bla Target 3
creator: Ilona Blank
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ..
mutable notes: .
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1150041_sequence/1

BBa_I13600
type: ComponentDefinition
persistent identity: BBa I13600
display id: BBa_I13600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet with CFP reporter (without LVA tag)
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0020
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I13600_sequence/1

BBa_E0020
type: Component
persistent identity: component947194
display id: component947194
version: 1
top level: BBa_I13600
access: public
definition: ecfp

BBa_B0010
type: Component
persistent identity: component947199
display id: component947199
version: 1
top level: BBa_I13600
access: public
definition: BBa_B0010

BBa_B0012
type: Component
persistent identity: component947209
display id: component947209
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component947192
display id: component947192
version: 1
top level: BBa_I13600
access: public
definition: BBa_B0034

BBa_R0040
type: Component
persistent identity: component947184
display id: component947184
version: 1
top level: BBa_I13600
access: public
definition: p(tetR)

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947209
display id: annotation947209
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947209/range947209/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13600/annotation947209/range947209/1
type: Range
persistent identity: range947209
display id: range947209
version: 1
direction: direction/forward
top level: BBa_I13600
start: 900
end: 940
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947199
display id: annotation947199
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947199/range947199/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13600/annotation947199/range947199/1
type: Range
persistent identity: range947199
display id: range947199
version: 1
direction: direction/forward
top level: BBa_I13600
start: 812
end: 891
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation947194
display id: annotation947194
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947194/range947194/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13600/annotation947194/range947194/1
type: Range
persistent identity: range947194
display id: range947194
version: 1
direction: direction/forward
top level: BBa_I13600
start: 81
end: 803
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947192
display id: annotation947192
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947192/range947192/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13600/annotation947192/range947192/1
type: Range
persistent identity: range947192
display id: range947192
version: 1
direction: direction/forward
top level: BBa_I13600
start: 63
end: 74
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947184
display id: annotation947184
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947184/range947184/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13600/annotation947184/range947184/1
type: Range
persistent identity: range947184
display id: range947184
version: 1
direction: direction/forward
top level: BBa_I13600
start: 1
end: 54
orientation: inline

BBa_K118019
type: ComponentDefinition
persistent identity: BBa K118019
display id: BBa_K118019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Plac+rbs+glgC (ADP-glucose pyrophosphorylase)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of glgC from ''Escherichia coli'' JM109 (see [http://partsregistry.org/wiki/index.php?title=Part:BBa_K118015 BBa_K118015]) with a ribosome binding site coupled to an upstream Lac promoter and ''lacZ''' as a method for testing expression.
mutable notes: No special considerations
mutable provenance: ''Escherichia coli'' JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K118015
component: BBa_J33207
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118015
sequence annotation: BBa_J33207
sequence: https://synbiohub.org/public/igem/BBa_K118019_sequence/1

BBa_K118015
type: Component
persistent identity: component1978666
display id: component1978666
version: 1
top level: BBa_K118019
access: public
definition: https://synbiohub.org/public/igem/BBa_K118015/1

BBa_J33207
type: Component
persistent identity: component1978660
display id: component1978660
version: 1
top level: BBa_K118019
access: public
definition: https://synbiohub.org/public/igem/BBa_J33207/1

BBa_J15001
type: Component
persistent identity: component1978663
display id: component1978663
version: 1
top level: BBa_K118019
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978663
display id: annotation1978663
version: 1
top level: BBa_K118019
location: https://synbiohub.org/public/igem/BBa_K118019/annotation1978663/range1978663/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118019/annotation1978663/range1978663/1
type: Range
persistent identity: range1978663
display id: range1978663
version: 1
direction: direction/reverse
top level: BBa_K118019
start: 609
end: 618
orientation: inline

BBa_K118015
type: SequenceAnnotation
persistent identity: annotation1978666
display id: annotation1978666
version: 1
top level: BBa_K118019
location: https://synbiohub.org/public/igem/BBa_K118019/annotation1978666/range1978666/1
component: BBa_K118015

https://synbiohub.org/public/igem/BBa_K118019/annotation1978666/range1978666/1
type: Range
persistent identity: range1978666
display id: range1978666
version: 1
direction: direction/reverse
top level: BBa_K118019
start: 625
end: 1923
orientation: inline

BBa_J33207
type: SequenceAnnotation
persistent identity: annotation1978660
display id: annotation1978660
version: 1
top level: BBa_K118019
location: https://synbiohub.org/public/igem/BBa_K118019/annotation1978660/range1978660/1
component: BBa_J33207

https://synbiohub.org/public/igem/BBa_K118019/annotation1978660/range1978660/1
type: Range
persistent identity: range1978660
display id: range1978660
version: 1
direction: direction/reverse
top level: BBa_K118019
start: 1
end: 600
orientation: inline

BBa_K1150040
type: ComponentDefinition
persistent identity: BBa K1150040
display id: BBa_K1150040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: uniCAS RNAimer to Bla Target 2
creator: Ilona Blank
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1150040_sequence/1

cII p22
type: ComponentDefinition
persistent identity: BBa R0053
display id: BBa_R0053
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (p22 cII regulated)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it. This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)
mutable provenance: Bacteriophage p22.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: -35
sequence annotation: -10
sequence annotation: OR3
sequence annotation: BBa_R0053
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_R0053_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2037
display id: annotation2037
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2037/range2037/1
type: Range
persistent identity: range2037
display id: range2037
version: 1
direction: direction/forward
top level: cII p22
start: 34
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2038
display id: annotation2038
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2038/range2038/1
type: Range
persistent identity: range2038
display id: range2038
version: 1
direction: direction/forward
top level: cII p22
start: 18
end: 23
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2042
display id: annotation2042
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2042/range2042/1
type: Range
persistent identity: range2042
display id: range2042
version: 1
direction: direction/forward
top level: cII p22
start: 30
end: 35
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2041
display id: annotation2041
version: 1
top level: cII p22
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2041/range2041/1
type: Range
persistent identity: range2041
display id: range2041
version: 1
direction: direction/forward
top level: cII p22
start: 8
end: 13
orientation: inline

OR3
type: SequenceAnnotation
persistent identity: annotation2035
display id: annotation2035
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2035/range2035/1
type: Range
persistent identity: range2035
display id: range2035
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 3
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation7069
display id: annotation7069
version: 1
top level: cII p22
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1

https://synbiohub.org/public/igem/BBa_R0053/annotation7069/range7069/1
type: Range
persistent identity: range7069
display id: range7069
version: 1
direction: direction/forward
top level: cII p22
start: 1
end: 54
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2036
display id: annotation2036
version: 1
top level: cII p22
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1

https://synbiohub.org/public/igem/BBa_R0053/annotation2036/range2036/1
type: Range
persistent identity: range2036
display id: range2036
version: 1
direction: direction/forward
top level: cII p22
start: 11
end: 28
orientation: inline

BBa_K1150042
type: ComponentDefinition
persistent identity: BBa K1150042
display id: BBa_K1150042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: uniCAS RNaimer to Bla Target 4
creator: Ilona Blank
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: .
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1150042_sequence/1

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for AHL
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
mutable provenance: V. fischeri AF170104
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: BBa_C0061
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
direction: direction/forward
top level: luxI
start: 580
end: 611
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 618
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
direction: direction/forward
top level: luxI
start: 619
end: 643
orientation: inline

BBa_P0151
type: ComponentDefinition
persistent identity: BBa P0151
display id: BBa_P0151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (lambda) [S0165]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI from lambda. Used as the input section for Quad Part Inverters Q01150 and Q01151.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0031
component: BBa_B0012
component: BBa_B0010
component: BBa_C0051
sequence annotation: BBa_B0031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_P0151_sequence/1

BBa_B0031
type: Component
persistent identity: component944503
display id: component944503
version: 1
top level: BBa_P0151
access: public
definition: BBa_B0031

BBa_B0012
type: Component
persistent identity: component944529
display id: component944529
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944519
display id: component944519
version: 1
top level: BBa_P0151
access: public
definition: BBa_B0010

BBa_C0051
type: Component
persistent identity: component944513
display id: component944513
version: 1
top level: BBa_P0151
access: public
definition: cI lam

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944503
display id: annotation944503
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
type: Range
persistent identity: range944503
display id: range944503
version: 1
direction: direction/forward
top level: BBa_P0151
start: 1
end: 14
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944519
display id: annotation944519
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
type: Range
persistent identity: range944519
display id: range944519
version: 1
direction: direction/forward
top level: BBa_P0151
start: 804
end: 883
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944529
display id: annotation944529
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
type: Range
persistent identity: range944529
display id: range944529
version: 1
direction: direction/forward
top level: BBa_P0151
start: 892
end: 932
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation944513
display id: annotation944513
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
type: Range
persistent identity: range944513
display id: range944513
version: 1
direction: direction/forward
top level: BBa_P0151
start: 21
end: 770
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI repressor from E. coli (+LVA)
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lacI-LVA
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0012
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
direction: direction/forward
top level: lacI
start: 1129
end: 1153
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
direction: direction/forward
top level: lacI
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
direction: direction/forward
top level: lacI
start: 1090
end: 1128
orientation: inline

BBa_J52040
type: ComponentDefinition
persistent identity: BBa J52040
display id: BBa_J52040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMV + GFP-PEST191
creator: Monika Ciglic
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is made of constitutive promotor CMV and GFP-linker-PEST191 which acts as a reporter linked to degradation tag (PEST191).
mutable notes: This compositive part is cloned in vector BBa_J52017 (we have made it by ligating eucaryotic terminator in BioBrick vector pSB1AK3)
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J52028
component: BBa_J52034
sequence annotation: BBa_J52028
sequence annotation: BBa_J52034
sequence: https://synbiohub.org/public/igem/BBa_J52040_sequence/1

BBa_J52028
type: Component
persistent identity: component1903194
display id: component1903194
version: 1
top level: BBa_J52040
access: public
definition: https://synbiohub.org/public/igem/BBa_J52028/1

BBa_J52034
type: Component
persistent identity: component1903189
display id: component1903189
version: 1
top level: BBa_J52040
access: public
definition: https://synbiohub.org/public/igem/BBa_J52034/1

BBa_J52028
type: SequenceAnnotation
persistent identity: annotation1903194
display id: annotation1903194
version: 1
top level: BBa_J52040
location: https://synbiohub.org/public/igem/BBa_J52040/annotation1903194/range1903194/1
component: BBa_J52028

https://synbiohub.org/public/igem/BBa_J52040/annotation1903194/range1903194/1
type: Range
persistent identity: range1903194
display id: range1903194
version: 1
direction: direction/reverse
top level: BBa_J52040
start: 661
end: 1881
orientation: inline

BBa_J52034
type: SequenceAnnotation
persistent identity: annotation1903189
display id: annotation1903189
version: 1
top level: BBa_J52040
location: https://synbiohub.org/public/igem/BBa_J52040/annotation1903189/range1903189/1
component: BBa_J52034

https://synbiohub.org/public/igem/BBa_J52040/annotation1903189/range1903189/1
type: Range
persistent identity: range1903189
display id: range1903189
version: 1
direction: direction/reverse
top level: BBa_J52040
start: 1
end: 654
orientation: inline

BBa_I13602
type: ComponentDefinition
persistent identity: BBa I13602
display id: BBa_I13602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet operator with CFP reporter (with LVA tag) [R/Tc+]
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_E0022
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_E0022
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13602_sequence/1

BBa_B0012
type: Component
persistent identity: component947292
display id: component947292
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component947254
display id: component947254
version: 1
top level: BBa_I13602
access: public
definition: p(tetR)

BBa_B0010
type: Component
persistent identity: component947282
display id: component947282
version: 1
top level: BBa_I13602
access: public
definition: BBa_B0010

BBa_E0022
type: Component
persistent identity: component947274
display id: component947274
version: 1
top level: BBa_I13602
access: public
definition: ECFP

BBa_B0034
type: Component
persistent identity: component947262
display id: component947262
version: 1
top level: BBa_I13602
access: public
definition: BBa_B0034

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947282
display id: annotation947282
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947282/range947282/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13602/annotation947282/range947282/1
type: Range
persistent identity: range947282
display id: range947282
version: 1
direction: direction/forward
top level: BBa_I13602
start: 851
end: 930
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947292
display id: annotation947292
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947292/range947292/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13602/annotation947292/range947292/1
type: Range
persistent identity: range947292
display id: range947292
version: 1
direction: direction/forward
top level: BBa_I13602
start: 939
end: 979
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947254
display id: annotation947254
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947254/range947254/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13602/annotation947254/range947254/1
type: Range
persistent identity: range947254
display id: range947254
version: 1
direction: direction/forward
top level: BBa_I13602
start: 1
end: 54
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation947274
display id: annotation947274
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947274/range947274/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I13602/annotation947274/range947274/1
type: Range
persistent identity: range947274
display id: range947274
version: 1
direction: direction/forward
top level: BBa_I13602
start: 81
end: 842
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947262
display id: annotation947262
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947262/range947262/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13602/annotation947262/range947262/1
type: Range
persistent identity: range947262
display id: range947262
version: 1
direction: direction/forward
top level: BBa_I13602
start: 63
end: 74
orientation: inline

BBa_K1061003
type: ComponentDefinition
persistent identity: BBa K1061003
display id: BBa_K1061003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RIP 3
creator: mengyi sun
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:08:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1369_
m user id: 0
owner id: 11892
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: Receptor interacting protein 3, also a serine/threonine kinase that play an important role in cell apoptosis and necrosis. It can induce necrosis via the interaction with RIP 1.
mutable notes: Although RIP 3 can also induce apoptosis, it mainly induces necrosis via interaction with RIP 1. So the mammalian circuit that aid to use in clinical should use tightly control expression device.
mutable provenance: We clone this gene by RT-PCR.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1061003_sequence/1

BBa_K1150016
type: ComponentDefinition
persistent identity: BBa K1150016
display id: BBa_K1150016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: HA tag
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ..
mutable notes: ..
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: HA
sequence: https://synbiohub.org/public/igem/BBa_K1150016_sequence/1

HA
type: SequenceAnnotation
persistent identity: annotation2347937
display id: annotation2347937
version: 1
top level: BBa_K1150016
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K1150016/annotation2347937/range2347937/1

https://synbiohub.org/public/igem/BBa_K1150016/annotation2347937/range2347937/1
type: Range
persistent identity: range2347937
display id: range2347937
version: 1
direction: direction/forward
top level: BBa_K1150016
start: 1
end: 27
orientation: inline

T7pol
type: ComponentDefinition
persistent identity: BBa K145001
display id: BBa_K145001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNA polymerase
creator: Jonas Demeulemeester and Benjamien Moeyaert
created: 2008-07-07T11:00:00Z
modified: 2015-05-08T01:10:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: T7 RNA polymerase with LVA tag
mutable notes: Fuse the LVA tag to the T7 coding region
mutable provenance: BBa_I712022 LVA tag from BBa_E0022
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: protein
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145001_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1968216
display id: annotation1968216
version: 1
top level: T7pol
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968216/range1968216/1

https://synbiohub.org/public/igem/BBa_K145001/annotation1968216/range1968216/1
type: Range
persistent identity: range1968216
display id: range1968216
version: 1
direction: direction/forward
top level: T7pol
start: 1
end: 3
orientation: inline

protein
type: SequenceAnnotation
persistent identity: annotation1968243
display id: annotation1968243
version: 1
top level: T7pol
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968243/range1968243/1

https://synbiohub.org/public/igem/BBa_K145001/annotation1968243/range1968243/1
type: Range
persistent identity: range1968243
display id: range1968243
version: 1
direction: direction/forward
top level: T7pol
start: 4
end: 2649
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968218
display id: annotation1968218
version: 1
top level: T7pol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968218/range1968218/1

https://synbiohub.org/public/igem/BBa_K145001/annotation1968218/range1968218/1
type: Range
persistent identity: range1968218
display id: range1968218
version: 1
direction: direction/forward
top level: T7pol
start: 2653
end: 2655
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968217
display id: annotation1968217
version: 1
top level: T7pol
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145001/annotation1968217/range1968217/1

https://synbiohub.org/public/igem/BBa_K145001/annotation1968217/range1968217/1
type: Range
persistent identity: range1968217
display id: range1968217
version: 1
direction: direction/forward
top level: T7pol
start: 2650
end: 2652
orientation: inline

BBa_I746375
type: ComponentDefinition
persistent identity: BBa I746375
display id: BBa_I746375
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P2 ogr inducing PLL promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the P2 ogr activator on the PLL promoter. Note that the I746350 does contain a RBS.
mutable notes: I746313 was put upstream of I746325
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746313
component: BBa_I746325
sequence annotation: BBa_I746325
sequence annotation: BBa_I746313
sequence: https://synbiohub.org/public/igem/BBa_I746375_sequence/1

BBa_I746313
type: Component
persistent identity: component2377023
display id: component2377023
version: 1
top level: BBa_I746375
access: public
definition: https://synbiohub.org/public/igem/BBa_I746313/1

BBa_I746325
type: Component
persistent identity: component2377036
display id: component2377036
version: 1
top level: BBa_I746375
access: public
definition: https://synbiohub.org/public/igem/BBa_I746325/1

BBa_I746325
type: SequenceAnnotation
persistent identity: annotation2377036
display id: annotation2377036
version: 1
top level: BBa_I746375
location: https://synbiohub.org/public/igem/BBa_I746375/annotation2377036/range2377036/1
component: BBa_I746325

https://synbiohub.org/public/igem/BBa_I746375/annotation2377036/range2377036/1
type: Range
persistent identity: range2377036
display id: range2377036
version: 1
direction: direction/reverse
top level: BBa_I746375
start: 2333
end: 3307
orientation: inline

BBa_I746313
type: SequenceAnnotation
persistent identity: annotation2377023
display id: annotation2377023
version: 1
top level: BBa_I746375
location: https://synbiohub.org/public/igem/BBa_I746375/annotation2377023/range2377023/1
component: BBa_I746313

https://synbiohub.org/public/igem/BBa_I746375/annotation2377023/range2377023/1
type: Range
persistent identity: range2377023
display id: range2377023
version: 1
direction: direction/reverse
top level: BBa_I746375
start: 1
end: 2324
orientation: inline

cjBlue
type: ComponentDefinition
persistent identity: BBa K592011
display id: BBa_K592011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cjBlue, green chromoprotein
creator: Antonio Ascue Avalos
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This chromoprotein, cjBlue, naturally exhibits strong green/blue color when expressed. Compared to amilCP(BBa_K592009) and amilGFP(BBa_K592010), the color development is slower. On agar plates and in liquid culture, the color is readily visible to naked eye after 24-48 hours of incubation. The DNA was codon-optimized for expression in E.coli and synthesized by the Korean company Bioneer Corporation.
mutable notes: Codon-optimization for expression in E.coli necessary.
mutable provenance: Cnidopus japonicus
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cjBlue
sequence: https://synbiohub.org/public/igem/BBa_K592011_sequence/1

cjBlue
type: SequenceAnnotation
persistent identity: annotation2131787
display id: annotation2131787
version: 1
top level: cjBlue
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K592011/annotation2131787/range2131787/1

https://synbiohub.org/public/igem/BBa_K592011/annotation2131787/range2131787/1
type: Range
persistent identity: range2131787
display id: range2131787
version: 1
direction: direction/forward
top level: cjBlue
start: 1
end: 699
orientation: inline

BBa_J23109
type: ComponentDefinition
persistent identity: BBa J23109
display id: BBa_J23109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable description: Later
mutable notes: N/A
mutable provenance: Later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23109_sequence/1

BBa_I712058
type: ComponentDefinition
persistent identity: BBa I712058
display id: BBa_I712058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CMVp - myri - TEVP - GFP
creator: Katja Kolar
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1883
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Signal sequence for myristoilation fused with proteolytic cleavage site for TEV protease and mCerulean, all under control of CMV promoter.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I712004
component: BBa_I712016
component: BBa_J52642
sequence annotation: BBa_J52642
sequence annotation: BBa_I712016
sequence annotation: BBa_I712004
sequence: https://synbiohub.org/public/igem/BBa_I712058_sequence/1

BBa_I712004
type: Component
persistent identity: component1953738
display id: component1953738
version: 1
top level: BBa_I712058
access: public
definition: https://synbiohub.org/public/igem/BBa_I712004/1

BBa_I712016
type: Component
persistent identity: component1953739
display id: component1953739
version: 1
top level: BBa_I712058
access: public
definition: https://synbiohub.org/public/igem/BBa_I712016/1

BBa_J52642
type: Component
persistent identity: component1953743
display id: component1953743
version: 1
top level: BBa_I712058
access: public
definition: https://synbiohub.org/public/igem/BBa_J52642/1

BBa_J52642
type: SequenceAnnotation
persistent identity: annotation1953743
display id: annotation1953743
version: 1
top level: BBa_I712058
location: https://synbiohub.org/public/igem/BBa_I712058/annotation1953743/range1953743/1
component: BBa_J52642

https://synbiohub.org/public/igem/BBa_I712058/annotation1953743/range1953743/1
type: Range
persistent identity: range1953743
display id: range1953743
version: 1
direction: direction/reverse
top level: BBa_I712058
start: 724
end: 1443
orientation: inline

BBa_I712016
type: SequenceAnnotation
persistent identity: annotation1953739
display id: annotation1953739
version: 1
top level: BBa_I712058
location: https://synbiohub.org/public/igem/BBa_I712058/annotation1953739/range1953739/1
component: BBa_I712016

https://synbiohub.org/public/igem/BBa_I712058/annotation1953739/range1953739/1
type: Range
persistent identity: range1953739
display id: range1953739
version: 1
direction: direction/reverse
top level: BBa_I712058
start: 661
end: 717
orientation: inline

BBa_I712004
type: SequenceAnnotation
persistent identity: annotation1953738
display id: annotation1953738
version: 1
top level: BBa_I712058
location: https://synbiohub.org/public/igem/BBa_I712058/annotation1953738/range1953738/1
component: BBa_I712004

https://synbiohub.org/public/igem/BBa_I712058/annotation1953738/range1953738/1
type: Range
persistent identity: range1953738
display id: range1953738
version: 1
direction: direction/reverse
top level: BBa_I712058
start: 1
end: 654
orientation: inline

T7
type: ComponentDefinition
persistent identity: BBa K145014
display id: BBa_K145014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 RNA Polymerase with UmuD derived tag
creator: Benjamien Moeyaert
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:10:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2939
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: T7 RNA Polymerase with N-terminal UmuD degradation tag.
mutable notes: This part is different from the simple composition of the tag and the polymerase, as there cannot be a stop codon in the scar.
mutable provenance: Part composed of the T7 RNA Polymerase and the N-terminal degradation tag, but with no scar (no stop codon)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop
sequence annotation: tag
sequence annotation: start
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K145014_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1968239
display id: annotation1968239
version: 1
top level: T7
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968239/range1968239/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968239/range1968239/1
type: Range
persistent identity: range1968239
display id: range1968239
version: 1
direction: direction/forward
top level: T7
start: 2740
end: 2742
orientation: inline

tag
type: SequenceAnnotation
persistent identity: annotation1968241
display id: annotation1968241
version: 1
top level: T7
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968241/range1968241/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968241/range1968241/1
type: Range
persistent identity: range1968241
display id: range1968241
version: 1
direction: direction/forward
top level: T7
start: 1
end: 87
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1968240
display id: annotation1968240
version: 1
top level: T7
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968240/range1968240/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968240/range1968240/1
type: Range
persistent identity: range1968240
display id: range1968240
version: 1
direction: direction/forward
top level: T7
start: 2737
end: 2739
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1968238
display id: annotation1968238
version: 1
top level: T7
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968238/range1968238/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968238/range1968238/1
type: Range
persistent identity: range1968238
display id: range1968238
version: 1
direction: direction/forward
top level: T7
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1968242
display id: annotation1968242
version: 1
top level: T7
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K145014/annotation1968242/range1968242/1

https://synbiohub.org/public/igem/BBa_K145014/annotation1968242/range1968242/1
type: Range
persistent identity: range1968242
display id: range1968242
version: 1
direction: direction/forward
top level: T7
start: 88
end: 2736
orientation: inline

Cse1
type: ComponentDefinition
persistent identity: BBa K1218003
display id: BBa_K1218003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CRISPR CasA E. coli (Modern)
creator: Trevor Kalkus, Gordon Wade, Alissa Greenberg
created: 2013-08-27T11:00:00Z
modified: 2015-05-08T01:09:42Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1532_
m user id: 0
owner id: 17712
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: The E. coli CRISPR system uses the CASCADE complex to target invasive DNA. CasA is the first subunit of the CASCADE complex and is responsible for the initial identification of invasive DNA. This part can be used with casBCDE to create the full CASCADE protein complex.
mutable notes: We had to perform mutagenesis twice to remove PstI cut sites.
mutable provenance: E. coli DH5alpha genome
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1218003_sequence/1

BBa_K115020
type: ComponentDefinition
persistent identity: BBa K115020
display id: BBa_K115020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RNA thermometer (ROSE hairpin 37??C)
creator: Bastiaan van den Berg
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:09:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: An RNA thermometer that theoretically switches on translation at 37 degrees Celcius and switched of translation below that temperature. Still to be tested.
mutable notes: later.
mutable provenance: ROSE hairpin.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: SD
sequence: https://synbiohub.org/public/igem/BBa_K115020_sequence/1

SD
type: SequenceAnnotation
persistent identity: annotation1972923
display id: annotation1972923
version: 1
top level: BBa_K115020
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K115020/annotation1972923/range1972923/1

https://synbiohub.org/public/igem/BBa_K115020/annotation1972923/range1972923/1
type: Range
persistent identity: range1972923
display id: range1972923
version: 1
direction: direction/forward
top level: BBa_K115020
start: 24
end: 29
orientation: inline

BBa_K1150010
type: ComponentDefinition
persistent identity: BBa K1150010
display id: BBa_K1150010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NLS
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: This NLS..
mutable notes: ..
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
sequence annotation: NLS
sequence: https://synbiohub.org/public/igem/BBa_K1150010_sequence/1

NLS
type: SequenceAnnotation
persistent identity: annotation2347465
display id: annotation2347465
version: 1
top level: BBa_K1150010
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1150010/annotation2347465/range2347465/1

https://synbiohub.org/public/igem/BBa_K1150010/annotation2347465/range2347465/1
type: Range
persistent identity: range2347465
display id: range2347465
version: 1
direction: direction/forward
top level: BBa_K1150010
start: 1
end: 21
orientation: inline

BBa_S03749
type: ComponentDefinition
persistent identity: BBa S03749
display id: BBa_S03749
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tet-regulated cro (Strong tetR promoter)
creator: Deborah Jiang
created: 2007-07-19T11:00:00Z
modified: 2015-05-08T01:14:29Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _115_
m user id: 0
owner id: 1907
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I759002
component: BBa_S03745
sequence annotation: BBa_S03745
sequence annotation: BBa_I759002
sequence: https://synbiohub.org/public/igem/BBa_S03749_sequence/1

BBa_I759002
type: Component
persistent identity: component2270715
display id: component2270715
version: 1
top level: BBa_S03749
access: public
definition: https://synbiohub.org/public/igem/BBa_I759002/1

BBa_S03745
type: Component
persistent identity: component2270738
display id: component2270738
version: 1
top level: BBa_S03749
access: public
definition: https://synbiohub.org/public/igem/BBa_S03745/1

BBa_S03745
type: SequenceAnnotation
persistent identity: annotation2270738
display id: annotation2270738
version: 1
top level: BBa_S03749
location: https://synbiohub.org/public/igem/BBa_S03749/annotation2270738/range2270738/1
component: BBa_S03745

https://synbiohub.org/public/igem/BBa_S03749/annotation2270738/range2270738/1
type: Range
persistent identity: range2270738
display id: range2270738
version: 1
direction: direction/reverse
top level: BBa_S03749
start: 321
end: 1340
orientation: inline

BBa_I759002
type: SequenceAnnotation
persistent identity: annotation2270715
display id: annotation2270715
version: 1
top level: BBa_S03749
location: https://synbiohub.org/public/igem/BBa_S03749/annotation2270715/range2270715/1
component: BBa_I759002

https://synbiohub.org/public/igem/BBa_S03749/annotation2270715/range2270715/1
type: Range
persistent identity: range2270715
display id: range2270715
version: 1
direction: direction/reverse
top level: BBa_S03749
start: 1
end: 312
orientation: inline

BBa_B0025
type: ComponentDefinition
persistent identity: BBa B0025
display id: BBa_B0025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0015), reversed
creator: Caitlin Conboy
created: 2003-12-02T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B0010
sequence annotation: B0012
sequence: https://synbiohub.org/public/igem/BBa_B0025_sequence/1

B0010
type: SequenceAnnotation
persistent identity: annotation369703
display id: annotation369703
version: 1
top level: BBa_B0025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1

https://synbiohub.org/public/igem/BBa_B0025/annotation369703/range369703/1
type: Range
persistent identity: range369703
display id: range369703
version: 1
direction: direction/reverse
top level: BBa_B0025
start: 50
end: 129
orientation: inline

B0012
type: SequenceAnnotation
persistent identity: annotation369702
display id: annotation369702
version: 1
top level: BBa_B0025
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1

https://synbiohub.org/public/igem/BBa_B0025/annotation369702/range369702/1
type: Range
persistent identity: range369702
display id: range369702
version: 1
direction: direction/reverse
top level: BBa_B0025
start: 1
end: 41
orientation: inline

BBa_J23102
type: ComponentDefinition
persistent identity: BBa J23102
display id: BBa_J23102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: replace later
mutable notes: N/A
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23102_sequence/1

BBa_E0044
type: ComponentDefinition
persistent identity: BBa E0044
display id: BBa_E0044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)
creator: jcbraff
created: 2004-09-29T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP-AAV [Pst1 site in earlier version knocked out]
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0044_sequence/1

BBa_K747094
type: ComponentDefinition
persistent identity: BBa K747094
display id: BBa_K747094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TAL-Protein_TG6_Direpeat
creator: Lucas Schneider
created: 2012-09-24T11:00:00Z
modified: 2015-05-08T01:13:11Z
discontinued: false
dominant: false
group u list: _998_
m user id: 0
owner id: 13224
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: follows
mutable notes: This protein domain can only be used within the TAL-effector-toolkit designed by the IGEM2012 Team Freiburg. The codon usage was modified to reduce homology in the sequence. A silent point mutation was inserted into the BsmBI restriction site in the chloramphenicol gene.
mutable provenance: This Part was synthesized and flanked with BsmBI-restriction sites.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: TC 6
sequence: https://synbiohub.org/public/igem/BBa_K747094_sequence/1

TC 6
type: SequenceAnnotation
persistent identity: annotation2197072
display id: annotation2197072
version: 1
top level: BBa_K747094
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_K747094/annotation2197072/range2197072/1

https://synbiohub.org/public/igem/BBa_K747094/annotation2197072/range2197072/1
type: Range
persistent identity: range2197072
display id: range2197072
version: 1
direction: direction/forward
top level: BBa_K747094
start: 14
end: 211
orientation: inline

RNAimer
type: ComponentDefinition
persistent identity: BBa K1150034
display id: BBa_K1150034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dCas RNA plasmid: "uniCAS RNAimer"
creator: Fenja Gawlas, Philipp Schwenk
created: 2013-09-16T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: ..
mutable notes: ..
mutable provenance: ..
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: BbsI cutting site
sequence annotation: pH1
sequence annotation: direct repeat 1
sequence annotation: pU6
sequence annotation: direct repeat 2
sequence annotation: tracrRNA
sequence annotation: Dummy crRNA
sequence: https://synbiohub.org/public/igem/BBa_K1150034_sequence/1

BbsI cutting site
type: SequenceAnnotation
persistent identity: annotation2357483
display id: annotation2357483
version: 1
top level: RNAimer
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1150034/annotation2357483/range2357483/1

https://synbiohub.org/public/igem/BBa_K1150034/annotation2357483/range2357483/1
type: Range
persistent identity: range2357483
display id: range2357483
version: 1
direction: direction/forward
top level: RNAimer
start: 746
end: 752
orientation: inline

pH1
type: SequenceAnnotation
persistent identity: annotation2357477
display id: annotation2357477
version: 1
top level: RNAimer
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1150034/annotation2357477/range2357477/1

https://synbiohub.org/public/igem/BBa_K1150034/annotation2357477/range2357477/1
type: Range
persistent identity: range2357477
display id: range2357477
version: 1
direction: direction/forward
top level: RNAimer
start: 1
end: 230
orientation: inline

direct repeat 1
type: SequenceAnnotation
persistent identity: annotation2357480
display id: annotation2357480
version: 1
top level: RNAimer
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1150034/annotation2357480/range2357480/1

https://synbiohub.org/public/igem/BBa_K1150034/annotation2357480/range2357480/1
type: Range
persistent identity: range2357480
display id: range2357480
version: 1
direction: direction/forward
top level: RNAimer
start: 700
end: 736
orientation: inline

pU6
type: SequenceAnnotation
persistent identity: annotation2357479
display id: annotation2357479
version: 1
top level: RNAimer
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K1150034/annotation2357479/range2357479/1

https://synbiohub.org/public/igem/BBa_K1150034/annotation2357479/range2357479/1
type: Range
persistent identity: range2357479
display id: range2357479
version: 1
direction: direction/forward
top level: RNAimer
start: 449
end: 698
orientation: inline

direct repeat 2
type: SequenceAnnotation
persistent identity: annotation2357484
display id: annotation2357484
version: 1
top level: RNAimer
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1150034/annotation2357484/range2357484/1

https://synbiohub.org/public/igem/BBa_K1150034/annotation2357484/range2357484/1
type: Range
persistent identity: range2357484
display id: range2357484
version: 1
direction: direction/forward
top level: RNAimer
start: 752
end: 788
orientation: inline

tracrRNA
type: SequenceAnnotation
persistent identity: annotation2357478
display id: annotation2357478
version: 1
top level: RNAimer
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1150034/annotation2357478/range2357478/1

https://synbiohub.org/public/igem/BBa_K1150034/annotation2357478/range2357478/1
type: Range
persistent identity: range2357478
display id: range2357478
version: 1
direction: direction/forward
top level: RNAimer
start: 231
end: 317
orientation: inline

Dummy crRNA
type: SequenceAnnotation
persistent identity: annotation2357481
display id: annotation2357481
version: 1
top level: RNAimer
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1150034/annotation2357481/range2357481/1

https://synbiohub.org/public/igem/BBa_K1150034/annotation2357481/range2357481/1
type: Range
persistent identity: range2357481
display id: range2357481
version: 1
direction: direction/forward
top level: RNAimer
start: 736
end: 752
orientation: inline

BbsI cutting site
type: SequenceAnnotation
persistent identity: annotation2357482
display id: annotation2357482
version: 1
top level: RNAimer
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1150034/annotation2357482/range2357482/1

https://synbiohub.org/public/igem/BBa_K1150034/annotation2357482/range2357482/1
type: Range
persistent identity: range2357482
display id: range2357482
version: 1
direction: direction/forward
top level: RNAimer
start: 738
end: 744
orientation: inline

BBa_I712019
type: ComponentDefinition
persistent identity: BBa I712019
display id: BBa_I712019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Firefly luciferase - luciferase from Photinus pyralis
creator: Anja Korenčič
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
mutable notes: Part is cloned into pSBAK3 vector with terminator.
mutable provenance: This firefly luciferase comes from Promega PGL3 vector.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I712019_sequence/1

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: later
mutable notes: N/A
mutable provenance: later
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K1184000
type: ComponentDefinition
persistent identity: BBa K1184000
display id: BBa_K1184000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: KillerRed
creator: Eric Pederson
created: 2013-09-03T11:00:00Z
modified: 2016-01-25T02:40:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1497_
m user id: 4206
owner id: 12713
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable description: KillerRed is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of yellow-orange light (540-585 nm). KillerRed is engineered from Aequorea victoria GFP (avGFP) to be phototoxic. It has been shown that KillerRed produces superoxide radical anions by reacting with water. Superoxide reacts with the chromophore of KillerRed, causing it to become dark, which ultimately gives rise to a bleaching effect. KillerRed is spectrally similar to mRFP1 with a similar brightness. KillerRed is oligomeric and may form large aggregates. This sequence is codon optimized for mammalian cells and has 14 rare proline codons for E. coli (CCC) and one rare arginine codon (AGA). Codon optimization should be taken into consideration if large amounts of the protein are required. Expression on a high-copy plasmid has produced detectable fluorescent signal, however.
mutable notes: This sequence is not codon optimized for E. coli.
mutable provenance: Engineered from avGFP. Provided by the Bruchez lab at Carnegie Mellon
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Chromophore
sequence annotation: Phototoxic Mutation Ser(121)
sequence annotation: Phototoxic Mutation Glu(70)
sequence annotation: Phototoxic Mutation Ala(163)
sequence annotation: Phototoxic Mutation Asn(147)
sequence annotation: Double Stop
sequence annotation: cds
sequence annotation: Start
sequence: https://synbiohub.org/public/igem/BBa_K1184000_sequence/1

Chromophore
type: SequenceAnnotation
persistent identity: annotation2334958
display id: annotation2334958
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334958/range2334958/1
type: Range
persistent identity: range2334958
display id: range2334958
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 199
end: 207
orientation: inline

Phototoxic Mutation Ser(121)
type: SequenceAnnotation
persistent identity: annotation2334957
display id: annotation2334957
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334957/range2334957/1
type: Range
persistent identity: range2334957
display id: range2334957
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 367
end: 369
orientation: inline

Phototoxic Mutation Glu(70)
type: SequenceAnnotation
persistent identity: annotation2334956
display id: annotation2334956
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334956/range2334956/1
type: Range
persistent identity: range2334956
display id: range2334956
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 208
end: 210
orientation: inline

Phototoxic Mutation Ala(163)
type: SequenceAnnotation
persistent identity: annotation2334965
display id: annotation2334965
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334965/range2334965/1
type: Range
persistent identity: range2334965
display id: range2334965
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 487
end: 489
orientation: inline

Phototoxic Mutation Asn(147)
type: SequenceAnnotation
persistent identity: annotation2334960
display id: annotation2334960
version: 1
top level: BBa_K1184000
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334960/range2334960/1
type: Range
persistent identity: range2334960
display id: range2334960
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 439
end: 441
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation2334953
display id: annotation2334953
version: 1
top level: BBa_K1184000
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334953/range2334953/1
type: Range
persistent identity: range2334953
display id: range2334953
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 718
end: 723
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2334955
display id: annotation2334955
version: 1
top level: BBa_K1184000
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334955/range2334955/1
type: Range
persistent identity: range2334955
display id: range2334955
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 717
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2334954
display id: annotation2334954
version: 1
top level: BBa_K1184000
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1

https://synbiohub.org/public/igem/BBa_K1184000/annotation2334954/range2334954/1
type: Range
persistent identity: range2334954
display id: range2334954
version: 1
direction: direction/forward
top level: BBa_K1184000
start: 1
end: 3
orientation: inline

BBa_J45219
type: ComponentDefinition
persistent identity: BBa J45219
display id: BBa_J45219
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Banana odor enzyme (ATF1) generator
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is an intermediate to the protein generator BBa_45220. This part is almost identical to BBa_J45199 except that it uses B0032 as its RBS (instead of B0030).
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_J45014
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_J45014
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45219_sequence/1

BBa_B0032
type: Component
persistent identity: component1908023
display id: component1908023
version: 1
top level: BBa_J45219
access: public
definition: BBa_B0032

BBa_J45014
type: Component
persistent identity: component1908029
display id: component1908029
version: 1
top level: BBa_J45219
access: public
definition: https://synbiohub.org/public/igem/BBa_J45014/1

BBa_B0012
type: Component
persistent identity: component1908032
display id: component1908032
version: 1
top level: BBa_J45219
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1908030
display id: component1908030
version: 1
top level: BBa_J45219
access: public
definition: BBa_B0010

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1908029
display id: annotation1908029
version: 1
top level: BBa_J45219
location: https://synbiohub.org/public/igem/BBa_J45219/annotation1908029/range1908029/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45219/annotation1908029/range1908029/1
type: Range
persistent identity: range1908029
display id: range1908029
version: 1
direction: direction/reverse
top level: BBa_J45219
start: 20
end: 1600
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1908023
display id: annotation1908023
version: 1
top level: BBa_J45219
location: https://synbiohub.org/public/igem/BBa_J45219/annotation1908023/range1908023/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45219/annotation1908023/range1908023/1
type: Range
persistent identity: range1908023
display id: range1908023
version: 1
direction: direction/forward
top level: BBa_J45219
start: 1
end: 13
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1908030
display id: annotation1908030
version: 1
top level: BBa_J45219
location: https://synbiohub.org/public/igem/BBa_J45219/annotation1908030/range1908030/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45219/annotation1908030/range1908030/1
type: Range
persistent identity: range1908030
display id: range1908030
version: 1
direction: direction/forward
top level: BBa_J45219
start: 1609
end: 1688
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1908032
display id: annotation1908032
version: 1
top level: BBa_J45219
location: https://synbiohub.org/public/igem/BBa_J45219/annotation1908032/range1908032/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45219/annotation1908032/range1908032/1
type: Range
persistent identity: range1908032
display id: range1908032
version: 1
direction: direction/forward
top level: BBa_J45219
start: 1697
end: 1737
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.4 (weaker) -- derivative of BBa_0030
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
direction: direction/forward
top level: BBa_B0033
start: 7
end: 10
orientation: inline

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
direction: direction/forward
top level: BBa_B0033
start: 1
end: 11
orientation: inline

BBa_K118024
type: ComponentDefinition
persistent identity: BBa K118024
display id: BBa_K118024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dxs+LIMS1+appY
creator: Andrew Hall
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: ''dxs'', from ''Escherichia coli'' JM109, encodes 1-deoxyxylulose-5-phosphate synthase, thiamine-requiring, which catalyses the first step in the biosynthesis of terpenoids. Overexpression of ''dxs'' has been reported to increase yields of carotenoids and other terpenoids [add reference here]. ''lims1'', from ''Citrus limon'', encodes limonene synthase, which produces (+)-limonene from farnesyl diphosphate (an intermediate in the mevalonate pathway). (+)-Limonene is a component of lemon scent. ''appY'', from ''Escherichia coli'' JM109, encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of the gene has been reported to result in enhanced lycopene production by ''E. coli'' (Kang ''et al.'', 2005). (All three genes are individually preceded by a ribosome binding site.)
mutable notes: The order of the genes in the operon corresponds to our perceived importance of each gene, bearing in mind that translation is more efficient of the genes early in the mRNA.
mutable provenance: ''dxs'' and ''appY'' are both from ''E. coli'' JM109 genomic DNA. ''lims1'' is from ''Citrus limon'' genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K118000
component: BBa_J15001
component: BBa_I742110
component: BBa_K118001
sequence annotation: BBa_K118000
sequence annotation: BBa_J15001
sequence annotation: BBa_I742110
sequence annotation: BBa_K118001
sequence: https://synbiohub.org/public/igem/BBa_K118024_sequence/1

BBa_K118000
type: Component
persistent identity: component1978670
display id: component1978670
version: 1
top level: BBa_K118024
access: public
definition: dxs

BBa_J15001
type: Component
persistent identity: component1978669
display id: component1978669
version: 1
top level: BBa_K118024
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_I742110
type: Component
persistent identity: component1978677
display id: component1978677
version: 1
top level: BBa_K118024
access: public
definition: https://synbiohub.org/public/igem/BBa_I742110/1

BBa_K118001
type: Component
persistent identity: component1978681
display id: component1978681
version: 1
top level: BBa_K118024
access: public
definition: https://synbiohub.org/public/igem/BBa_K118001/1

BBa_J15001
type: Component
persistent identity: component1978680
display id: component1978680
version: 1
top level: BBa_K118024
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: Component
persistent identity: component1978673
display id: component1978673
version: 1
top level: BBa_K118024
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118000
type: SequenceAnnotation
persistent identity: annotation1978670
display id: annotation1978670
version: 1
top level: BBa_K118024
location: https://synbiohub.org/public/igem/BBa_K118024/annotation1978670/range1978670/1
component: BBa_K118000

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type: Range
persistent identity: range1978670
display id: range1978670
version: 1
direction: direction/reverse
top level: BBa_K118024
start: 17
end: 1882
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978673
display id: annotation1978673
version: 1
top level: BBa_K118024
location: https://synbiohub.org/public/igem/BBa_K118024/annotation1978673/range1978673/1
component: BBa_J15001

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type: Range
persistent identity: range1978673
display id: range1978673
version: 1
direction: direction/reverse
top level: BBa_K118024
start: 1891
end: 1900
orientation: inline

BBa_I742110
type: SequenceAnnotation
persistent identity: annotation1978677
display id: annotation1978677
version: 1
top level: BBa_K118024
location: https://synbiohub.org/public/igem/BBa_K118024/annotation1978677/range1978677/1
component: BBa_I742110

https://synbiohub.org/public/igem/BBa_K118024/annotation1978677/range1978677/1
type: Range
persistent identity: range1978677
display id: range1978677
version: 1
direction: direction/reverse
top level: BBa_K118024
start: 1907
end: 3577
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978669
display id: annotation1978669
version: 1
top level: BBa_K118024
location: https://synbiohub.org/public/igem/BBa_K118024/annotation1978669/range1978669/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118024/annotation1978669/range1978669/1
type: Range
persistent identity: range1978669
display id: range1978669
version: 1
direction: direction/reverse
top level: BBa_K118024
start: 1
end: 10
orientation: inline

BBa_K118001
type: SequenceAnnotation
persistent identity: annotation1978681
display id: annotation1978681
version: 1
top level: BBa_K118024
location: https://synbiohub.org/public/igem/BBa_K118024/annotation1978681/range1978681/1
component: BBa_K118001

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type: Range
persistent identity: range1978681
display id: range1978681
version: 1
direction: direction/reverse
top level: BBa_K118024
start: 3602
end: 4354
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978680
display id: annotation1978680
version: 1
top level: BBa_K118024
location: https://synbiohub.org/public/igem/BBa_K118024/annotation1978680/range1978680/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118024/annotation1978680/range1978680/1
type: Range
persistent identity: range1978680
display id: range1978680
version: 1
direction: direction/reverse
top level: BBa_K118024
start: 3586
end: 3595
orientation: inline

R6K
type: ComponentDefinition
persistent identity: BBa J61001
display id: BBa_J61001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [R6K] Origin of replication
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature. {pSB1A2-Bca1011}
mutable notes: N/A
mutable provenance: PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is Bca1011 ---- ca1011F Forward Biobricking of R6K GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg ca1011R Reverse biobricking of R6K ccgctactagtaGCAGTTCAACCTGTTGATAG
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: R6K
sequence: https://synbiohub.org/public/igem/BBa_J61001_sequence/1

R6K
type: SequenceAnnotation
persistent identity: annotation1892271
display id: annotation1892271
version: 1
top level: R6K
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1

https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1
type: Range
persistent identity: range1892271
display id: range1892271
version: 1
direction: direction/forward
top level: R6K
start: 27
end: 406
orientation: inline

BBa_P0153
type: ComponentDefinition
persistent identity: BBa P0153
display id: BBa_P0153
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cII (p22) [S0167]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cII from p22. Used as the input section for Quad Part Inverters Q01530.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0053
component: BBa_B0012
component: BBa_B0010
component: BBa_B0031
sequence annotation: BBa_B0010
sequence annotation: BBa_C0053
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence: https://synbiohub.org/public/igem/BBa_P0153_sequence/1

BBa_C0053
type: Component
persistent identity: component944580
display id: component944580
version: 1
top level: BBa_P0153
access: public
definition: c2 P22

BBa_B0012
type: Component
persistent identity: component944598
display id: component944598
version: 1
top level: BBa_P0153
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944588
display id: component944588
version: 1
top level: BBa_P0153
access: public
definition: BBa_B0010

BBa_B0031
type: Component
persistent identity: component944568
display id: component944568
version: 1
top level: BBa_P0153
access: public
definition: BBa_B0031

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944588
display id: annotation944588
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944588/range944588/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0153/annotation944588/range944588/1
type: Range
persistent identity: range944588
display id: range944588
version: 1
direction: direction/forward
top level: BBa_P0153
start: 741
end: 820
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation944580
display id: annotation944580
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944580/range944580/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_P0153/annotation944580/range944580/1
type: Range
persistent identity: range944580
display id: range944580
version: 1
direction: direction/forward
top level: BBa_P0153
start: 21
end: 707
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944598
display id: annotation944598
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944598/range944598/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0153/annotation944598/range944598/1
type: Range
persistent identity: range944598
display id: range944598
version: 1
direction: direction/forward
top level: BBa_P0153
start: 829
end: 869
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944568
display id: annotation944568
version: 1
top level: BBa_P0153
location: https://synbiohub.org/public/igem/BBa_P0153/annotation944568/range944568/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0153/annotation944568/range944568/1
type: Range
persistent identity: range944568
display id: range944568
version: 1
direction: direction/forward
top level: BBa_P0153
start: 1
end: 14
orientation: inline

BBa_K1150013
type: ComponentDefinition
persistent identity: BBa K1150013
display id: BBa_K1150013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AGS Linker
creator: Freiburg 2013
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1462_
m user id: 0
owner id: 16627
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable description: ...
mutable notes: ...
mutable provenance: ...
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AGS Linker
sequence: https://synbiohub.org/public/igem/BBa_K1150013_sequence/1

AGS Linker
type: SequenceAnnotation
persistent identity: annotation2347934
display id: annotation2347934
version: 1
top level: BBa_K1150013
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K1150013/annotation2347934/range2347934/1

https://synbiohub.org/public/igem/BBa_K1150013/annotation2347934/range2347934/1
type: Range
persistent identity: range2347934
display id: range2347934
version: 1
direction: direction/forward
top level: BBa_K1150013
start: 1
end: 9
orientation: inline

BBa_J23070
type: ComponentDefinition
persistent identity: BBa J23070
display id: BBa_J23070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ser2 derived key
creator: bryan hernandez
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:39Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 936
owning group id: 52
sample status: In stock
status: status/Available
bookmark: true
mutable description: contains the J23060 loop on a tRNA. more on this later
mutable notes: no
mutable provenance: Ser2 tRNA and J23060
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: binding site
sequence annotation: loop
sequence: https://synbiohub.org/public/igem/BBa_J23070_sequence/1

binding site
type: SequenceAnnotation
persistent identity: annotation1897362
display id: annotation1897362
version: 1
top level: BBa_J23070
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J23070/annotation1897362/range1897362/1

https://synbiohub.org/public/igem/BBa_J23070/annotation1897362/range1897362/1
type: Range
persistent identity: range1897362
display id: range1897362
version: 1
direction: direction/forward
top level: BBa_J23070
start: 43
end: 69
orientation: inline

loop
type: SequenceAnnotation
persistent identity: annotation1897361
display id: annotation1897361
version: 1
top level: BBa_J23070
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J23070/annotation1897361/range1897361/1

https://synbiohub.org/public/igem/BBa_J23070/annotation1897361/range1897361/1
type: Range
persistent identity: range1897361
display id: range1897361
version: 1
direction: direction/forward
top level: BBa_J23070
start: 37
end: 74
orientation: inline

BBa_J52029
type: ComponentDefinition
persistent identity: BBa J52029
display id: BBa_J52029
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: NF-kappaB + GFP-PEST191
creator: Jelka Pohar
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 855
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reporter GFP-linker-PEST191 under NF-kappaB inducible promoter. Used for detection of promoter activity and degradation of GFP.
mutable notes: Promoter has a NotI restriction site. We did not alter DNA sequence, because this site did not affect our part construction and function of promoter. This part is cloned in plasmid with terminator BBa_J52017.
mutable provenance: NF-kappaB - GFP-PEST191 - already described (see BBa_J52028)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J52028
component: BBa_J52010
sequence annotation: BBa_J52028
sequence annotation: BBa_J52010
sequence: https://synbiohub.org/public/igem/BBa_J52029_sequence/1

BBa_J52028
type: Component
persistent identity: component1903075
display id: component1903075
version: 1
top level: BBa_J52029
access: public
definition: https://synbiohub.org/public/igem/BBa_J52028/1

BBa_J52010
type: Component
persistent identity: component1903070
display id: component1903070
version: 1
top level: BBa_J52029
access: public
definition: https://synbiohub.org/public/igem/BBa_J52010/1

BBa_J52028
type: SequenceAnnotation
persistent identity: annotation1903075
display id: annotation1903075
version: 1
top level: BBa_J52029
location: https://synbiohub.org/public/igem/BBa_J52029/annotation1903075/range1903075/1
component: BBa_J52028

https://synbiohub.org/public/igem/BBa_J52029/annotation1903075/range1903075/1
type: Range
persistent identity: range1903075
display id: range1903075
version: 1
direction: direction/reverse
top level: BBa_J52029
start: 821
end: 2041
orientation: inline

BBa_J52010
type: SequenceAnnotation
persistent identity: annotation1903070
display id: annotation1903070
version: 1
top level: BBa_J52029
location: https://synbiohub.org/public/igem/BBa_J52029/annotation1903070/range1903070/1
component: BBa_J52010

https://synbiohub.org/public/igem/BBa_J52029/annotation1903070/range1903070/1
type: Range
persistent identity: range1903070
display id: range1903070
version: 1
direction: direction/reverse
top level: BBa_J52029
start: 1
end: 814
orientation: inline