iGEM 2018 Distribution Plate 3 Well 18A
type: Collection
persistent identity: iGEM 2018 Plate3 Well18A
display id: iGEM_2018_Plate3_Well18A
version: 1
owned by: myers
member: BBa_K081000

iGEM 2018 Distribution Plate 3 Well 23J
type: Collection
persistent identity: iGEM 2018 Plate3 Well23J
display id: iGEM_2018_Plate3_Well23J
version: 1
owned by: myers
member: BBa_S0103

iGEM 2018 Distribution Plate 3 Well 9E
type: Collection
persistent identity: iGEM 2018 Plate3 Well9E
display id: iGEM_2018_Plate3_Well9E
version: 1
owned by: myers
member: BBa_I732081

iGEM 2018 Distribution Plate 3 Well 24G
type: Collection
persistent identity: iGEM 2018 Plate3 Well24G
display id: iGEM_2018_Plate3_Well24G
version: 1
owned by: myers
member: BBa_K079050

iGEM 2018 Distribution Plate 3 Well 17H
type: Collection
persistent identity: iGEM 2018 Plate3 Well17H
display id: iGEM_2018_Plate3_Well17H
version: 1
owned by: myers
member: P(Lac) IQ

iGEM 2018 Distribution Plate 3 Well 15O
type: Collection
persistent identity: iGEM 2018 Plate3 Well15O
display id: iGEM_2018_Plate3_Well15O
version: 1
owned by: myers
member: BBa_I13500

iGEM 2018 Distribution Plate 3 Well 7E
type: Collection
persistent identity: iGEM 2018 Plate3 Well7E
display id: iGEM_2018_Plate3_Well7E
version: 1
owned by: myers
member: BBa_I13450

iGEM 2018 Distribution Plate 3 Well 20E
type: Collection
persistent identity: iGEM 2018 Plate3 Well20E
display id: iGEM_2018_Plate3_Well20E
version: 1
owned by: myers
member: BBa_K118015

iGEM 2018 Distribution Plate 3 Well 3C
type: Collection
persistent identity: iGEM 2018 Plate3 Well3C
display id: iGEM_2018_Plate3_Well3C
version: 1
owned by: myers
member: BBa_S01022

iGEM 2018 Distribution Plate 3 Well 13D
type: Collection
persistent identity: iGEM 2018 Plate3 Well13D
display id: iGEM_2018_Plate3_Well13D
version: 1
owned by: myers
member: Cat

iGEM 2018 Distribution Plate 3 Well 16A
type: Collection
persistent identity: iGEM 2018 Plate3 Well16A
display id: iGEM_2018_Plate3_Well16A
version: 1
owned by: myers
member: BBa_K091100

iGEM 2018 Distribution Plate 3 Well 24E
type: Collection
persistent identity: iGEM 2018 Plate3 Well24E
display id: iGEM_2018_Plate3_Well24E
version: 1
owned by: myers
member: cI HK022

iGEM 2018 Distribution Plate 3 Well 1M
type: Collection
persistent identity: iGEM 2018 Plate3 Well1M
display id: iGEM_2018_Plate3_Well1M
version: 1
owned by: myers
member: BBa_E5501

iGEM 2018 Distribution Plate 3 Well 3O
type: Collection
persistent identity: iGEM 2018 Plate3 Well3O
display id: iGEM_2018_Plate3_Well3O
version: 1
owned by: myers
member: PenI

iGEM 2018 Distribution Plate 3 Well 20G
type: Collection
persistent identity: iGEM 2018 Plate3 Well20G
display id: iGEM_2018_Plate3_Well20G
version: 1
owned by: myers
member: BBa_K118016

iGEM 2018 Distribution Plate 3 Well 3B
type: Collection
persistent identity: iGEM 2018 Plate3 Well3B
display id: iGEM_2018_Plate3_Well3B
version: 1
owned by: myers
member: lacZ-alpha

iGEM 2018 Distribution Plate 3 Well 24J
type: Collection
persistent identity: iGEM 2018 Plate3 Well24J
display id: iGEM_2018_Plate3_Well24J
version: 1
owned by: myers
member: BBa_J24675

iGEM 2018 Distribution Plate 3 Well 21D
type: Collection
persistent identity: iGEM 2018 Plate3 Well21D
display id: iGEM_2018_Plate3_Well21D
version: 1
owned by: myers
member: BBa_K081019

iGEM 2018 Distribution Plate 3 Well 11J
type: Collection
persistent identity: iGEM 2018 Plate3 Well11J
display id: iGEM_2018_Plate3_Well11J
version: 1
owned by: myers
member: Zif23

iGEM 2018 Distribution Plate 3 Well 18M
type: Collection
persistent identity: iGEM 2018 Plate3 Well18M
display id: iGEM_2018_Plate3_Well18M
version: 1
owned by: myers
member: BBa_K118002

iGEM 2018 Distribution Plate 3 Well 21J
type: Collection
persistent identity: iGEM 2018 Plate3 Well21J
display id: iGEM_2018_Plate3_Well21J
version: 1
owned by: myers
member: BBa_K084007

iGEM 2018 Distribution Plate 3 Well 9M
type: Collection
persistent identity: iGEM 2018 Plate3 Well9M
display id: iGEM_2018_Plate3_Well9M
version: 1
owned by: myers
member: BBa_I746324

iGEM 2018 Distribution Plate 3 Well 11P
type: Collection
persistent identity: iGEM 2018 Plate3 Well11P
display id: iGEM_2018_Plate3_Well11P
version: 1
owned by: myers
member: cheB

iGEM 2018 Distribution Plate 3 Well 23I
type: Collection
persistent identity: iGEM 2018 Plate3 Well23I
display id: iGEM_2018_Plate3_Well23I
version: 1
owned by: myers
member: BBa_I714033

iGEM 2018 Distribution Plate 3 Well 19M
type: Collection
persistent identity: iGEM 2018 Plate3 Well19M
display id: iGEM_2018_Plate3_Well19M
version: 1
owned by: myers
member: cI lam

iGEM 2018 Distribution Plate 3 Well 17O
type: Collection
persistent identity: iGEM 2018 Plate3 Well17O
display id: iGEM_2018_Plate3_Well17O
version: 1
owned by: myers
member: BBa_J23119

iGEM 2018 Distribution Plate 3 Well 4O
type: Collection
persistent identity: iGEM 2018 Plate3 Well4O
display id: iGEM_2018_Plate3_Well4O
version: 1
owned by: myers
member: BBa_F2620

iGEM 2018 Distribution Plate 3 Well 2D
type: Collection
persistent identity: iGEM 2018 Plate3 Well2D
display id: iGEM_2018_Plate3_Well2D
version: 1
owned by: myers
member: BBa_I13401

iGEM 2018 Distribution Plate 3 Well 3J
type: Collection
persistent identity: iGEM 2018 Plate3 Well3J
display id: iGEM_2018_Plate3_Well3J
version: 1
owned by: myers
member: BBa_J52008

iGEM 2018 Distribution Plate 3 Well 4A
type: Collection
persistent identity: iGEM 2018 Plate3 Well4A
display id: iGEM_2018_Plate3_Well4A
version: 1
owned by: myers
member: COMT

iGEM 2018 Distribution Plate 3 Well 10O
type: Collection
persistent identity: iGEM 2018 Plate3 Well10O
display id: iGEM_2018_Plate3_Well10O
version: 1
owned by: myers
member: BBa_J33201

iGEM 2018 Distribution Plate 3 Well 23H
type: Collection
persistent identity: iGEM 2018 Plate3 Well23H
display id: iGEM_2018_Plate3_Well23H
version: 1
owned by: myers
member: BBa_S0102

iGEM 2018 Distribution Plate 3 Well 1K
type: Collection
persistent identity: iGEM 2018 Plate3 Well1K
display id: iGEM_2018_Plate3_Well1K
version: 1
owned by: myers
member: aiiA

iGEM 2018 Distribution Plate 3 Well 15F
type: Collection
persistent identity: iGEM 2018 Plate3 Well15F
display id: iGEM_2018_Plate3_Well15F
version: 1
owned by: myers
member: BBa_S03575

iGEM 2018 Distribution Plate 3 Well 18C
type: Collection
persistent identity: iGEM 2018 Plate3 Well18C
display id: iGEM_2018_Plate3_Well18C
version: 1
owned by: myers
member: BBa_K081001

iGEM 2018 Distribution Plate 3 Well 7F
type: Collection
persistent identity: iGEM 2018 Plate3 Well7F
display id: iGEM_2018_Plate3_Well7F
version: 1
owned by: myers
member: BBa_J52021

iGEM 2018 Distribution Plate 3 Well 13E
type: Collection
persistent identity: iGEM 2018 Plate3 Well13E
display id: iGEM_2018_Plate3_Well13E
version: 1
owned by: myers
member: BBa_I718009

iGEM 2018 Distribution Plate 3 Well 21E
type: Collection
persistent identity: iGEM 2018 Plate3 Well21E
display id: iGEM_2018_Plate3_Well21E
version: 1
owned by: myers
member: SV40 NLS

iGEM 2018 Distribution Plate 3 Well 1P
type: Collection
persistent identity: iGEM 2018 Plate3 Well1P
display id: iGEM_2018_Plate3_Well1P
version: 1
owned by: myers
member: BBa_I732094

iGEM 2018 Distribution Plate 3 Well 16O
type: Collection
persistent identity: iGEM 2018 Plate3 Well16O
display id: iGEM_2018_Plate3_Well16O
version: 1
owned by: myers
member: BBa_K081017

iGEM 2018 Distribution Plate 3 Well 15N
type: Collection
persistent identity: iGEM 2018 Plate3 Well15N
display id: iGEM_2018_Plate3_Well15N
version: 1
owned by: myers
member: BBa_P0451

iGEM 2018 Distribution Plate 3 Well 12M
type: Collection
persistent identity: iGEM 2018 Plate3 Well12M
display id: iGEM_2018_Plate3_Well12M
version: 1
owned by: myers
member: BBa_K165002

iGEM 2018 Distribution Plate 3 Well 3D
type: Collection
persistent identity: iGEM 2018 Plate3 Well3D
display id: iGEM_2018_Plate3_Well3D
version: 1
owned by: myers
member: BBa_I732085

iGEM 2018 Distribution Plate 3 Well 12F
type: Collection
persistent identity: iGEM 2018 Plate3 Well12F
display id: iGEM_2018_Plate3_Well12F
version: 1
owned by: myers
member: BBa_S01013

iGEM 2018 Distribution Plate 3 Well 20I
type: Collection
persistent identity: iGEM 2018 Plate3 Well20I
display id: iGEM_2018_Plate3_Well20I
version: 1
owned by: myers
member: BBa_K118021

iGEM 2018 Distribution Plate 3 Well 14C
type: Collection
persistent identity: iGEM 2018 Plate3 Well14C
display id: iGEM_2018_Plate3_Well14C
version: 1
owned by: myers
member: BBa_K206001

iGEM 2018 Distribution Plate 3 Well 24F
type: Collection
persistent identity: iGEM 2018 Plate3 Well24F
display id: iGEM_2018_Plate3_Well24F
version: 1
owned by: myers
member: BBa_S03814

iGEM 2018 Distribution Plate 3 Well 22H
type: Collection
persistent identity: iGEM 2018 Plate3 Well22H
display id: iGEM_2018_Plate3_Well22H
version: 1
owned by: myers
member: BBa_I13544

iGEM 2018 Distribution Plate 3 Well 14B
type: Collection
persistent identity: iGEM 2018 Plate3 Well14B
display id: iGEM_2018_Plate3_Well14B
version: 1
owned by: myers
member: BBa_I13261

iGEM 2018 Distribution Plate 3 Well 11G
type: Collection
persistent identity: iGEM 2018 Plate3 Well11G
display id: iGEM_2018_Plate3_Well11G
version: 1
owned by: myers
member: BBa_I714036

iGEM 2018 Distribution Plate 3 Well 17A
type: Collection
persistent identity: iGEM 2018 Plate3 Well17A
display id: iGEM_2018_Plate3_Well17A
version: 1
owned by: myers
member: mRFP1

iGEM 2018 Distribution Plate 3 Well 18J
type: Collection
persistent identity: iGEM 2018 Plate3 Well18J
display id: iGEM_2018_Plate3_Well18J
version: 1
owned by: myers
member: BBa_I746385

iGEM 2018 Distribution Plate 3 Well 3E
type: Collection
persistent identity: iGEM 2018 Plate3 Well3E
display id: iGEM_2018_Plate3_Well3E
version: 1
owned by: myers
member: OmpR

iGEM 2018 Distribution Plate 3
type: Collection
persistent identity: iGEM 2018 Plate3
display id: iGEM_2018_Plate3
version: 1
owned by: myers
member: iGEM 2018 Distribution Plate 3 Well 15M
member: BBa_M0100
member: BBa_I13202
member: mCherry
member: iGEM 2018 Distribution Plate 3 Well 5I
member: iGEM 2018 Distribution Plate 3 Well 8I
member: LacY
member: BBa_I732085
member: BBa_I13405
member: iGEM 2018 Distribution Plate 3 Well 11D
member: BBa_I13500
member: BBa_K137058
member: iGEM 2018 Distribution Plate 3 Well 2I
member: iGEM 2018 Distribution Plate 3 Well 10I
member: iGEM 2018 Distribution Plate 3 Well 17D
member: iGEM 2018 Distribution Plate 3 Well 14D
member: BBa_I13023
member: OmpR
member: iGEM 2018 Distribution Plate 3 Well 1N
member: BBa_J52019
member: BBa_P0451
member: BBa_K079050
member: iGEM 2018 Distribution Plate 3 Well 21L
member: BBa_K206001
member: ATF1
member: BBa_S03184
member: iGEM 2018 Distribution Plate 3 Well 13I
member: iGEM 2018 Distribution Plate 3 Well 16I
member: iGEM 2018 Distribution Plate 3 Well 19H
member: iGEM 2018 Distribution Plate 3 Well 19I
member: BBa_I715040
member: ARL4A0604
member: iGEM 2018 Distribution Plate 3 Well 24L
member: iGEM 2018 Distribution Plate 3 Well 20C
member: iGEM 2018 Distribution Plate 3 Well 23C
member: iGEM 2018 Distribution Plate 3 Well 7N
member: iGEM 2018 Distribution Plate 3 Well 4N
member: iGEM 2018 Distribution Plate 3 Well 13H
member: BBa_I732830
member: iGEM 2018 Distribution Plate 3 Well 21M
member: iGEM 2018 Distribution Plate 3 Well 10H
member: BBa_S03154
member: iGEM 2018 Distribution Plate 3 Well 16H
member: iGEM 2018 Distribution Plate 3 Well 22H
member: iGEM 2018 Distribution Plate 3 Well 18M
member: iGEM 2018 Distribution Plate 3 Well 7M
member: iGEM 2018 Distribution Plate 3 Well 12M
member: iGEM 2018 Distribution Plate 3 Well 1M
member: iGEM 2018 Distribution Plate 3 Well 4M
member: iGEM 2018 Distribution Plate 3 Well 14E
member: iGEM 2018 Distribution Plate 3 Well 7A
member: iGEM 2018 Distribution Plate 3 Well 20P
member: BBa_I1468
member: iGEM 2018 Distribution Plate 3 Well 4A
member: BBa_B0017
member: LasR+PAI
member: iGEM 2018 Distribution Plate 3 Well 23P
member: iGEM 2018 Distribution Plate 3 Well 1A
member: BBa_I746385
member: iGEM 2018 Distribution Plate 3 Well 11E
member: iGEM 2018 Distribution Plate 3 Well 17E
member: BBa_S03119
member: iGEM 2018 Distribution Plate 3 Well 15L
member: iGEM 2018 Distribution Plate 3 Well 18L
member: BBa_J24018
member: BBa_I0407
member: Zif23
member: BBa_J37032
member: BBa_S03858
member: cinR
member: iGEM 2018 Distribution Plate 3 Well 12A
member: iGEM 2018 Distribution Plate 3 Well 15A
member: Bla

iGEM 2018 Distribution Plate 3 Well 1H
type: Collection
persistent identity: iGEM 2018 Plate3 Well1H
display id: iGEM_2018_Plate3_Well1H
version: 1
owned by: myers
member: BBa_P0412

iGEM 2018 Distribution Plate 3 Well 4E
type: Collection
persistent identity: iGEM 2018 Plate3 Well4E
display id: iGEM_2018_Plate3_Well4E
version: 1
owned by: myers
member: BBa_J33204

iGEM 2018 Distribution Plate 3 Well 15H
type: Collection
persistent identity: iGEM 2018 Plate3 Well15H
display id: iGEM_2018_Plate3_Well15H
version: 1
owned by: myers
member: BBa_P0140

iGEM 2018 Distribution Plate 3 Well 21F
type: Collection
persistent identity: iGEM 2018 Plate3 Well21F
display id: iGEM_2018_Plate3_Well21F
version: 1
owned by: myers
member: amyE 5 IS

iGEM 2018 Distribution Plate 3 Well 7G
type: Collection
persistent identity: iGEM 2018 Plate3 Well7G
display id: iGEM_2018_Plate3_Well7G
version: 1
owned by: myers
member: BBa_I732815

iGEM 2018 Distribution Plate 3 Well 10J
type: Collection
persistent identity: iGEM 2018 Plate3 Well10J
display id: iGEM_2018_Plate3_Well10J
version: 1
owned by: myers
member: BBa_I6088

iGEM 2018 Distribution Plate 3 Well 2H
type: Collection
persistent identity: iGEM 2018 Plate3 Well2H
display id: iGEM_2018_Plate3_Well2H
version: 1
owned by: myers
member: zif23

iGEM 2018 Distribution Plate 3 Well 1N
type: Collection
persistent identity: iGEM 2018 Plate3 Well1N
display id: iGEM_2018_Plate3_Well1N
version: 1
owned by: myers
member: BBa_I732820

iGEM 2018 Distribution Plate 3 Well 19H
type: Collection
persistent identity: iGEM 2018 Plate3 Well19H
display id: iGEM_2018_Plate3_Well19H
version: 1
owned by: myers
member: RcsB

iGEM 2018 Distribution Plate 3 Well 6C
type: Collection
persistent identity: iGEM 2018 Plate3 Well6C
display id: iGEM_2018_Plate3_Well6C
version: 1
owned by: myers
member: BBa_B0017

iGEM 2018 Distribution Plate 3 Well 7B
type: Collection
persistent identity: iGEM 2018 Plate3 Well7B
display id: iGEM_2018_Plate3_Well7B
version: 1
owned by: myers
member: ARL2A0301

iGEM 2018 Distribution Plate 3 Well 20M
type: Collection
persistent identity: iGEM 2018 Plate3 Well20M
display id: iGEM_2018_Plate3_Well20M
version: 1
owned by: myers
member: BBa_K103003

iGEM 2018 Distribution Plate 3 Well 4D
type: Collection
persistent identity: iGEM 2018 Plate3 Well4D
display id: iGEM_2018_Plate3_Well4D
version: 1
owned by: myers
member: BBa_S03889

iGEM 2018 Distribution Plate 3 Well 9I
type: Collection
persistent identity: iGEM 2018 Plate3 Well9I
display id: iGEM_2018_Plate3_Well9I
version: 1
owned by: myers
member: BBa_I715039

iGEM 2018 Distribution Plate 3 Well 24L
type: Collection
persistent identity: iGEM 2018 Plate3 Well24L
display id: iGEM_2018_Plate3_Well24L
version: 1
owned by: myers
member: BBa_J06620

iGEM 2018 Distribution Plate 3 Well 22O
type: Collection
persistent identity: iGEM 2018 Plate3 Well22O
display id: iGEM_2018_Plate3_Well22O
version: 1
owned by: myers
member: BBa_K116638

iGEM 2018 Distribution Plate 3 Well 23L
type: Collection
persistent identity: iGEM 2018 Plate3 Well23L
display id: iGEM_2018_Plate3_Well23L
version: 1
owned by: myers
member: BBa_S0104

iGEM 2018 Distribution Plate 3 Well 18I
type: Collection
persistent identity: iGEM 2018 Plate3 Well18I
display id: iGEM_2018_Plate3_Well18I
version: 1
owned by: myers
member: BBa_K117002

iGEM 2018 Distribution Plate 3 Well 21H
type: Collection
persistent identity: iGEM 2018 Plate3 Well21H
display id: iGEM_2018_Plate3_Well21H
version: 1
owned by: myers
member: pTEF

iGEM 2018 Distribution Plate 3 Well 8I
type: Collection
persistent identity: iGEM 2018 Plate3 Well8I
display id: iGEM_2018_Plate3_Well8I
version: 1
owned by: myers
member: BBa_I746364

iGEM 2018 Distribution Plate 3 Well 14P
type: Collection
persistent identity: iGEM 2018 Plate3 Well14P
display id: iGEM_2018_Plate3_Well14P
version: 1
owned by: myers
member: BBa_I13516

iGEM 2018 Distribution Plate 3 Well 22L
type: Collection
persistent identity: iGEM 2018 Plate3 Well22L
display id: iGEM_2018_Plate3_Well22L
version: 1
owned by: myers
member: BBa_I13543

iGEM 2018 Distribution Plate 3 Well 22C
type: Collection
persistent identity: iGEM 2018 Plate3 Well22C
display id: iGEM_2018_Plate3_Well22C
version: 1
owned by: myers
member: BBa_K137040

iGEM 2018 Distribution Plate 3 Well 10P
type: Collection
persistent identity: iGEM 2018 Plate3 Well10P
display id: iGEM_2018_Plate3_Well10P
version: 1
owned by: myers
member: BBa_S01004

iGEM 2018 Distribution Plate 3 Well 12K
type: Collection
persistent identity: iGEM 2018 Plate3 Well12K
display id: iGEM_2018_Plate3_Well12K
version: 1
owned by: myers
member: BBa_K112806

iGEM 2018 Distribution Plate 3 Well 20F
type: Collection
persistent identity: iGEM 2018 Plate3 Well20F
display id: iGEM_2018_Plate3_Well20F
version: 1
owned by: myers
member: RQE Lambda

iGEM 2018 Distribution Plate 3 Well 12D
type: Collection
persistent identity: iGEM 2018 Plate3 Well12D
display id: iGEM_2018_Plate3_Well12D
version: 1
owned by: myers
member: BBa_S01008

iGEM 2018 Distribution Plate 3 Well 18L
type: Collection
persistent identity: iGEM 2018 Plate3 Well18L
display id: iGEM_2018_Plate3_Well18L
version: 1
owned by: myers
member: BBa_I746392

iGEM 2018 Distribution Plate 3 Well 3G
type: Collection
persistent identity: iGEM 2018 Plate3 Well3G
display id: iGEM_2018_Plate3_Well3G
version: 1
owned by: myers
member: cinR

iGEM 2018 Distribution Plate 3 Well 15D
type: Collection
persistent identity: iGEM 2018 Plate3 Well15D
display id: iGEM_2018_Plate3_Well15D
version: 1
owned by: myers
member: eyfp

iGEM 2018 Distribution Plate 3 Well 2M
type: Collection
persistent identity: iGEM 2018 Plate3 Well2M
display id: iGEM_2018_Plate3_Well2M
version: 1
owned by: myers
member: BBa_I741005

iGEM 2018 Distribution Plate 3 Well 8G
type: Collection
persistent identity: iGEM 2018 Plate3 Well8G
display id: iGEM_2018_Plate3_Well8G
version: 1
owned by: myers
member: BBa_I746361

iGEM 2018 Distribution Plate 3 Well 1O
type: Collection
persistent identity: iGEM 2018 Plate3 Well1O
display id: iGEM_2018_Plate3_Well1O
version: 1
owned by: myers
member: BBa_E5503

iGEM 2018 Distribution Plate 3 Well 5N
type: Collection
persistent identity: iGEM 2018 Plate3 Well5N
display id: iGEM_2018_Plate3_Well5N
version: 1
owned by: myers
member: ARL2A0101

iGEM 2018 Distribution Plate 3 Well 17N
type: Collection
persistent identity: iGEM 2018 Plate3 Well17N
display id: iGEM_2018_Plate3_Well17N
version: 1
owned by: myers
member: BBa_I11021

iGEM 2018 Distribution Plate 3 Well 19J
type: Collection
persistent identity: iGEM 2018 Plate3 Well19J
display id: iGEM_2018_Plate3_Well19J
version: 1
owned by: myers
member: BBa_J04500

iGEM 2018 Distribution Plate 3 Well 8E
type: Collection
persistent identity: iGEM 2018 Plate3 Well8E
display id: iGEM_2018_Plate3_Well8E
version: 1
owned by: myers
member: BBa_I746360

iGEM 2018 Distribution Plate 3 Well 6K
type: Collection
persistent identity: iGEM 2018 Plate3 Well6K
display id: iGEM_2018_Plate3_Well6K
version: 1
owned by: myers
member: rhIR

iGEM 2018 Distribution Plate 3 Well 8B
type: Collection
persistent identity: iGEM 2018 Plate3 Well8B
display id: iGEM_2018_Plate3_Well8B
version: 1
owned by: myers
member: BBa_I746001

iGEM 2018 Distribution Plate 3 Well 21A
type: Collection
persistent identity: iGEM 2018 Plate3 Well21A
display id: iGEM_2018_Plate3_Well21A
version: 1
owned by: myers
member: BBa_B0030

iGEM 2018 Distribution Plate 3 Well 13H
type: Collection
persistent identity: iGEM 2018 Plate3 Well13H
display id: iGEM_2018_Plate3_Well13H
version: 1
owned by: myers
member: BBa_I15009

iGEM 2018 Distribution Plate 3 Well 23P
type: Collection
persistent identity: iGEM 2018 Plate3 Well23P
display id: iGEM_2018_Plate3_Well23P
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 3 Well 18B
type: Collection
persistent identity: iGEM 2018 Plate3 Well18B
display id: iGEM_2018_Plate3_Well18B
version: 1
owned by: myers
member: BBa_I746370

iGEM 2018 Distribution Plate 3 Well 23G
type: Collection
persistent identity: iGEM 2018 Plate3 Well23G
display id: iGEM_2018_Plate3_Well23G
version: 1
owned by: myers
member: BBa_I746104

iGEM 2018 Distribution Plate 3 Well 11M
type: Collection
persistent identity: iGEM 2018 Plate3 Well11M
display id: iGEM_2018_Plate3_Well11M
version: 1
owned by: myers
member: BBa_I716210

iGEM 2018 Distribution Plate 3 Well 5I
type: Collection
persistent identity: iGEM 2018 Plate3 Well5I
display id: iGEM_2018_Plate3_Well5I
version: 1
owned by: myers
member: BBa_I13025

iGEM 2018 Distribution Plate 3 Well 6G
type: Collection
persistent identity: iGEM 2018 Plate3 Well6G
display id: iGEM_2018_Plate3_Well6G
version: 1
owned by: myers
member: BBa_I13521

iGEM 2018 Distribution Plate 3 Well 23B
type: Collection
persistent identity: iGEM 2018 Plate3 Well23B
display id: iGEM_2018_Plate3_Well23B
version: 1
owned by: myers
member: BBa_K081021

iGEM 2018 Distribution Plate 3 Well 7M
type: Collection
persistent identity: iGEM 2018 Plate3 Well7M
display id: iGEM_2018_Plate3_Well7M
version: 1
owned by: myers
member: BBa_I732074

iGEM 2018 Distribution Plate 3 Well 16N
type: Collection
persistent identity: iGEM 2018 Plate3 Well16N
display id: iGEM_2018_Plate3_Well16N
version: 1
owned by: myers
member: BBa_I13513

iGEM 2018 Distribution Plate 3 Well 22N
type: Collection
persistent identity: iGEM 2018 Plate3 Well22N
display id: iGEM_2018_Plate3_Well22N
version: 1
owned by: myers
member: BBa_I732906

iGEM 2018 Distribution Plate 3 Well 21M
type: Collection
persistent identity: iGEM 2018 Plate3 Well21M
display id: iGEM_2018_Plate3_Well21M
version: 1
owned by: myers
member: BBa_I13458

iGEM 2018 Distribution Plate 3 Well 4G
type: Collection
persistent identity: iGEM 2018 Plate3 Well4G
display id: iGEM_2018_Plate3_Well4G
version: 1
owned by: myers
member: LacI

iGEM 2018 Distribution Plate 3 Well 24I
type: Collection
persistent identity: iGEM 2018 Plate3 Well24I
display id: iGEM_2018_Plate3_Well24I
version: 1
owned by: myers
member: BBa_K145301

iGEM 2018 Distribution Plate 3 Well 16H
type: Collection
persistent identity: iGEM 2018 Plate3 Well16H
display id: iGEM_2018_Plate3_Well16H
version: 1
owned by: myers
member: BBa_I13974

iGEM 2018 Distribution Plate 3 Well 17F
type: Collection
persistent identity: iGEM 2018 Plate3 Well17F
display id: iGEM_2018_Plate3_Well17F
version: 1
owned by: myers
member: las TetO

iGEM 2018 Distribution Plate 3 Well 6P
type: Collection
persistent identity: iGEM 2018 Plate3 Well6P
display id: iGEM_2018_Plate3_Well6P
version: 1
owned by: myers
member: BBa_I732835

iGEM 2018 Distribution Plate 3 Well 20H
type: Collection
persistent identity: iGEM 2018 Plate3 Well20H
display id: iGEM_2018_Plate3_Well20H
version: 1
owned by: myers
member: BBa_J23040

iGEM 2018 Distribution Plate 3 Well 7I
type: Collection
persistent identity: iGEM 2018 Plate3 Well7I
display id: iGEM_2018_Plate3_Well7I
version: 1
owned by: myers
member: BBa_I732904

iGEM 2018 Distribution Plate 3 Well 19C
type: Collection
persistent identity: iGEM 2018 Plate3 Well19C
display id: iGEM_2018_Plate3_Well19C
version: 1
owned by: myers
member: BBa_I13600

iGEM 2018 Distribution Plate 3 Well 24D
type: Collection
persistent identity: iGEM 2018 Plate3 Well24D
display id: iGEM_2018_Plate3_Well24D
version: 1
owned by: myers
member: BBa_S03813

iGEM 2018 Distribution Plate 3 Well 10L
type: Collection
persistent identity: iGEM 2018 Plate3 Well10L
display id: iGEM_2018_Plate3_Well10L
version: 1
owned by: myers
member: BBa_I13512

iGEM 2018 Distribution Plate 3 Well 6I
type: Collection
persistent identity: iGEM 2018 Plate3 Well6I
display id: iGEM_2018_Plate3_Well6I
version: 1
owned by: myers
member: BBa_I13522

iGEM 2018 Distribution Plate 3 Well 13L
type: Collection
persistent identity: iGEM 2018 Plate3 Well13L
display id: iGEM_2018_Plate3_Well13L
version: 1
owned by: myers
member: BBa_I759002

iGEM 2018 Distribution Plate 3 Well 22B
type: Collection
persistent identity: iGEM 2018 Plate3 Well22B
display id: iGEM_2018_Plate3_Well22B
version: 1
owned by: myers
member: BBa_I13538

iGEM 2018 Distribution Plate 3 Well 2K
type: Collection
persistent identity: iGEM 2018 Plate3 Well2K
display id: iGEM_2018_Plate3_Well2K
version: 1
owned by: myers
member: BBa_I741018

iGEM 2018 Distribution Plate 3 Well 9C
type: Collection
persistent identity: iGEM 2018 Plate3 Well9C
display id: iGEM_2018_Plate3_Well9C
version: 1
owned by: myers
member: BBa_I732097

iGEM 2018 Distribution Plate 3 Well 10B
type: Collection
persistent identity: iGEM 2018 Plate3 Well10B
display id: iGEM_2018_Plate3_Well10B
version: 1
owned by: myers
member: LacY

iGEM 2018 Distribution Plate 3 Well 5O
type: Collection
persistent identity: iGEM 2018 Plate3 Well5O
display id: iGEM_2018_Plate3_Well5O
version: 1
owned by: myers
member: BBa_I13202

iGEM 2018 Distribution Plate 3 Well 24C
type: Collection
persistent identity: iGEM 2018 Plate3 Well24C
display id: iGEM_2018_Plate3_Well24C
version: 1
owned by: myers
member: BBa_K137058

iGEM 2018 Distribution Plate 3 Well 9B
type: Collection
persistent identity: iGEM 2018 Plate3 Well9B
display id: iGEM_2018_Plate3_Well9B
version: 1
owned by: myers
member: mnt (s)

iGEM 2018 Distribution Plate 3 Well 10G
type: Collection
persistent identity: iGEM 2018 Plate3 Well10G
display id: iGEM_2018_Plate3_Well10G
version: 1
owned by: myers
member: BBa_K203118

iGEM 2018 Distribution Plate 3 Well 2A
type: Collection
persistent identity: iGEM 2018 Plate3 Well2A
display id: iGEM_2018_Plate3_Well2A
version: 1
owned by: myers
member: BBa_I716155

iGEM 2018 Distribution Plate 3 Well 16D
type: Collection
persistent identity: iGEM 2018 Plate3 Well16D
display id: iGEM_2018_Plate3_Well16D
version: 1
owned by: myers
member: BBa_I13517

iGEM 2018 Distribution Plate 3 Well 4F
type: Collection
persistent identity: iGEM 2018 Plate3 Well4F
display id: iGEM_2018_Plate3_Well4F
version: 1
owned by: myers
member: BBa_S03184

iGEM 2018 Distribution Plate 3 Well 2I
type: Collection
persistent identity: iGEM 2018 Plate3 Well2I
display id: iGEM_2018_Plate3_Well2I
version: 1
owned by: myers
member: rbs+crtZ

iGEM 2018 Distribution Plate 3 Well 13J
type: Collection
persistent identity: iGEM 2018 Plate3 Well13J
display id: iGEM_2018_Plate3_Well13J
version: 1
owned by: myers
member: BBa_I759001

iGEM 2018 Distribution Plate 3 Well 11B
type: Collection
persistent identity: iGEM 2018 Plate3 Well11B
display id: iGEM_2018_Plate3_Well11B
version: 1
owned by: myers
member: araC

iGEM 2018 Distribution Plate 3 Well 14E
type: Collection
persistent identity: iGEM 2018 Plate3 Well14E
display id: iGEM_2018_Plate3_Well14E
version: 1
owned by: myers
member: BBa_K228001

iGEM 2018 Distribution Plate 3 Well 18K
type: Collection
persistent identity: iGEM 2018 Plate3 Well18K
display id: iGEM_2018_Plate3_Well18K
version: 1
owned by: myers
member: BBa_K117008

iGEM 2018 Distribution Plate 3 Well 10K
type: Collection
persistent identity: iGEM 2018 Plate3 Well10K
display id: iGEM_2018_Plate3_Well10K
version: 1
owned by: myers
member: Pr + GFP

iGEM 2018 Distribution Plate 3 Well 10N
type: Collection
persistent identity: iGEM 2018 Plate3 Well10N
display id: iGEM_2018_Plate3_Well10N
version: 1
owned by: myers
member: BBa_I13514

iGEM 2018 Distribution Plate 3 Well 13O
type: Collection
persistent identity: iGEM 2018 Plate3 Well13O
display id: iGEM_2018_Plate3_Well13O
version: 1
owned by: myers
member: BBa_I718015

iGEM 2018 Distribution Plate 3 Well 8P
type: Collection
persistent identity: iGEM 2018 Plate3 Well8P
display id: iGEM_2018_Plate3_Well8P
version: 1
owned by: myers
member: BBa_I20270

iGEM 2018 Distribution Plate 3 Well 8J
type: Collection
persistent identity: iGEM 2018 Plate3 Well8J
display id: iGEM_2018_Plate3_Well8J
version: 1
owned by: myers
member: ARL4A0604

iGEM 2018 Distribution Plate 3 Well 5D
type: Collection
persistent identity: iGEM 2018 Plate3 Well5D
display id: iGEM_2018_Plate3_Well5D
version: 1
owned by: myers
member: BBa_B1006

iGEM 2018 Distribution Plate 3 Well 18D
type: Collection
persistent identity: iGEM 2018 Plate3 Well18D
display id: iGEM_2018_Plate3_Well18D
version: 1
owned by: myers
member: BBa_I746371

iGEM 2018 Distribution Plate 3 Well 8A
type: Collection
persistent identity: iGEM 2018 Plate3 Well8A
display id: iGEM_2018_Plate3_Well8A
version: 1
owned by: myers
member: LacI

iGEM 2018 Distribution Plate 3 Well 17C
type: Collection
persistent identity: iGEM 2018 Plate3 Well17C
display id: iGEM_2018_Plate3_Well17C
version: 1
owned by: myers
member: BBa_I13504

iGEM 2018 Distribution Plate 3 Well 8D
type: Collection
persistent identity: iGEM 2018 Plate3 Well8D
display id: iGEM_2018_Plate3_Well8D
version: 1
owned by: myers
member: pLac+cI

iGEM 2018 Distribution Plate 3 Well 6L
type: Collection
persistent identity: iGEM 2018 Plate3 Well6L
display id: iGEM_2018_Plate3_Well6L
version: 1
owned by: myers
member: BBa_I732821

iGEM 2018 Distribution Plate 3 Well 1C
type: Collection
persistent identity: iGEM 2018 Plate3 Well1C
display id: iGEM_2018_Plate3_Well1C
version: 1
owned by: myers
member: EYFP

iGEM 2018 Distribution Plate 3 Well 21I
type: Collection
persistent identity: iGEM 2018 Plate3 Well21I
display id: iGEM_2018_Plate3_Well21I
version: 1
owned by: myers
member: BBa_J24679

iGEM 2018 Distribution Plate 3 Well 6J
type: Collection
persistent identity: iGEM 2018 Plate3 Well6J
display id: iGEM_2018_Plate3_Well6J
version: 1
owned by: myers
member: BBa_I13535

iGEM 2018 Distribution Plate 3 Well 16E
type: Collection
persistent identity: iGEM 2018 Plate3 Well16E
display id: iGEM_2018_Plate3_Well16E
version: 1
owned by: myers
member: BBa_K081005

iGEM 2018 Distribution Plate 3 Well 4C
type: Collection
persistent identity: iGEM 2018 Plate3 Well4C
display id: iGEM_2018_Plate3_Well4C
version: 1
owned by: myers
member: BBa_J22101

iGEM 2018 Distribution Plate 3 Well 9O
type: Collection
persistent identity: iGEM 2018 Plate3 Well9O
display id: iGEM_2018_Plate3_Well9O
version: 1
owned by: myers
member: BBa_I746105

iGEM 2018 Distribution Plate 3 Well 2B
type: Collection
persistent identity: iGEM 2018 Plate3 Well2B
display id: iGEM_2018_Plate3_Well2B
version: 1
owned by: myers
member: BBa_S0105

iGEM 2018 Distribution Plate 3 Well 5M
type: Collection
persistent identity: iGEM 2018 Plate3 Well5M
display id: iGEM_2018_Plate3_Well5M
version: 1
owned by: myers
member: BBa_I13028

iGEM 2018 Distribution Plate 3 Well 16I
type: Collection
persistent identity: iGEM 2018 Plate3 Well16I
display id: iGEM_2018_Plate3_Well16I
version: 1
owned by: myers
member: BBa_K081008

iGEM 2018 Distribution Plate 3 Well 9F
type: Collection
persistent identity: iGEM 2018 Plate3 Well9F
display id: iGEM_2018_Plate3_Well9F
version: 1
owned by: myers
member: penI

iGEM 2018 Distribution Plate 3 Well 9K
type: Collection
persistent identity: iGEM 2018 Plate3 Well9K
display id: iGEM_2018_Plate3_Well9K
version: 1
owned by: myers
member: BBa_I746320

iGEM 2018 Distribution Plate 3 Well 10H
type: Collection
persistent identity: iGEM 2018 Plate3 Well10H
display id: iGEM_2018_Plate3_Well10H
version: 1
owned by: myers
member: BBa_I6058

iGEM 2018 Distribution Plate 3 Well 22I
type: Collection
persistent identity: iGEM 2018 Plate3 Well22I
display id: iGEM_2018_Plate3_Well22I
version: 1
owned by: myers
member: BBa_K105004

iGEM 2018 Distribution Plate 3 Well 1D
type: Collection
persistent identity: iGEM 2018 Plate3 Well1D
display id: iGEM_2018_Plate3_Well1D
version: 1
owned by: myers
member: BBa_K118017

iGEM 2018 Distribution Plate 3 Well 20K
type: Collection
persistent identity: iGEM 2018 Plate3 Well20K
display id: iGEM_2018_Plate3_Well20K
version: 1
owned by: myers
member: BBa_K118027

iGEM 2018 Distribution Plate 3 Well 11O
type: Collection
persistent identity: iGEM 2018 Plate3 Well11O
display id: iGEM_2018_Plate3_Well11O
version: 1
owned by: myers
member: BBa_I716211

iGEM 2018 Distribution Plate 3 Well 24B
type: Collection
persistent identity: iGEM 2018 Plate3 Well24B
display id: iGEM_2018_Plate3_Well24B
version: 1
owned by: myers
member: T7rnap

iGEM 2018 Distribution Plate 3 Well 5P
type: Collection
persistent identity: iGEM 2018 Plate3 Well5P
display id: iGEM_2018_Plate3_Well5P
version: 1
owned by: myers
member: ARL2A0102

iGEM 2018 Distribution Plate 3 Well 14A
type: Collection
persistent identity: iGEM 2018 Plate3 Well14A
display id: iGEM_2018_Plate3_Well14A
version: 1
owned by: myers
member: pBAD

iGEM 2018 Distribution Plate 3 Well 7A
type: Collection
persistent identity: iGEM 2018 Plate3 Well7A
display id: iGEM_2018_Plate3_Well7A
version: 1
owned by: myers
member: BBa_A340620

iGEM 2018 Distribution Plate 3 Well 14O
type: Collection
persistent identity: iGEM 2018 Plate3 Well14O
display id: iGEM_2018_Plate3_Well14O
version: 1
owned by: myers
member: BBa_K145303

iGEM 2018 Distribution Plate 3 Well 5C
type: Collection
persistent identity: iGEM 2018 Plate3 Well5C
display id: iGEM_2018_Plate3_Well5C
version: 1
owned by: myers
member: LasR+PAI

iGEM 2018 Distribution Plate 3 Well 12O
type: Collection
persistent identity: iGEM 2018 Plate3 Well12O
display id: iGEM_2018_Plate3_Well12O
version: 1
owned by: myers
member: lexA DBD

iGEM 2018 Distribution Plate 3 Well 7K
type: Collection
persistent identity: iGEM 2018 Plate3 Well7K
display id: iGEM_2018_Plate3_Well7K
version: 1
owned by: myers
member: BBa_I732807

iGEM 2018 Distribution Plate 3 Well 9D
type: Collection
persistent identity: iGEM 2018 Plate3 Well9D
display id: iGEM_2018_Plate3_Well9D
version: 1
owned by: myers
member: mnt (w)

iGEM 2018 Distribution Plate 3 Well 23F
type: Collection
persistent identity: iGEM 2018 Plate3 Well23F
display id: iGEM_2018_Plate3_Well23F
version: 1
owned by: myers
member: BBa_E0420

iGEM 2018 Distribution Plate 3 Well 21K
type: Collection
persistent identity: iGEM 2018 Plate3 Well21K
display id: iGEM_2018_Plate3_Well21K
version: 1
owned by: myers
member: BBa_J09855

iGEM 2018 Distribution Plate 3 Well 16K
type: Collection
persistent identity: iGEM 2018 Plate3 Well16K
display id: iGEM_2018_Plate3_Well16K
version: 1
owned by: myers
member: BBa_K081009

iGEM 2018 Distribution Plate 3 Well 24A
type: Collection
persistent identity: iGEM 2018 Plate3 Well24A
display id: iGEM_2018_Plate3_Well24A
version: 1
owned by: myers
member: BBa_K116640

iGEM 2018 Distribution Plate 3 Well 18F
type: Collection
persistent identity: iGEM 2018 Plate3 Well18F
display id: iGEM_2018_Plate3_Well18F
version: 1
owned by: myers
member: BBa_I746372

iGEM 2018 Distribution Plate 3 Well 19I
type: Collection
persistent identity: iGEM 2018 Plate3 Well19I
display id: iGEM_2018_Plate3_Well19I
version: 1
owned by: myers
member: BBa_P0151

iGEM 2018 Distribution Plate 3 Well 11I
type: Collection
persistent identity: iGEM 2018 Plate3 Well11I
display id: iGEM_2018_Plate3_Well11I
version: 1
owned by: myers
member: RBS-LacY

iGEM 2018 Distribution Plate 3 Well 17J
type: Collection
persistent identity: iGEM 2018 Plate3 Well17J
display id: iGEM_2018_Plate3_Well17J
version: 1
owned by: myers
member: P22 attP

iGEM 2018 Distribution Plate 3 Well 5K
type: Collection
persistent identity: iGEM 2018 Plate3 Well5K
display id: iGEM_2018_Plate3_Well5K
version: 1
owned by: myers
member: BBa_I13027

iGEM 2018 Distribution Plate 3 Well 8H
type: Collection
persistent identity: iGEM 2018 Plate3 Well8H
display id: iGEM_2018_Plate3_Well8H
version: 1
owned by: myers
member: ARL2A0203

iGEM 2018 Distribution Plate 3 Well 12H
type: Collection
persistent identity: iGEM 2018 Plate3 Well12H
display id: iGEM_2018_Plate3_Well12H
version: 1
owned by: myers
member: BBa_S01012

iGEM 2018 Distribution Plate 3 Well 7H
type: Collection
persistent identity: iGEM 2018 Plate3 Well7H
display id: iGEM_2018_Plate3_Well7H
version: 1
owned by: myers
member: BBa_E2020

iGEM 2018 Distribution Plate 3 Well 10D
type: Collection
persistent identity: iGEM 2018 Plate3 Well10D
display id: iGEM_2018_Plate3_Well10D
version: 1
owned by: myers
member: BBa_K200008

iGEM 2018 Distribution Plate 3 Well 11K
type: Collection
persistent identity: iGEM 2018 Plate3 Well11K
display id: iGEM_2018_Plate3_Well11K
version: 1
owned by: myers
member: BBa_I716208

iGEM 2018 Distribution Plate 3 Well 11L
type: Collection
persistent identity: iGEM 2018 Plate3 Well11L
display id: iGEM_2018_Plate3_Well11L
version: 1
owned by: myers
member: Zif23

iGEM 2018 Distribution Plate 3 Well 5F
type: Collection
persistent identity: iGEM 2018 Plate3 Well5F
display id: iGEM_2018_Plate3_Well5F
version: 1
owned by: myers
member: BBa_B1007

iGEM 2018 Distribution Plate 3 Well 2J
type: Collection
persistent identity: iGEM 2018 Plate3 Well2J
display id: iGEM_2018_Plate3_Well2J
version: 1
owned by: myers
member: BBa_Q04530

iGEM 2018 Distribution Plate 3 Well 19K
type: Collection
persistent identity: iGEM 2018 Plate3 Well19K
display id: iGEM_2018_Plate3_Well19K
version: 1
owned by: myers
member: BBa_E0430

iGEM 2018 Distribution Plate 3 Well 1F
type: Collection
persistent identity: iGEM 2018 Plate3 Well1F
display id: iGEM_2018_Plate3_Well1F
version: 1
owned by: myers
member: BBa_K118018

iGEM 2018 Distribution Plate 3 Well 19N
type: Collection
persistent identity: iGEM 2018 Plate3 Well19N
display id: iGEM_2018_Plate3_Well19N
version: 1
owned by: myers
member: BBa_K082003

iGEM 2018 Distribution Plate 3 Well 9J
type: Collection
persistent identity: iGEM 2018 Plate3 Well9J
display id: iGEM_2018_Plate3_Well9J
version: 1
owned by: myers
member: cinI

iGEM 2018 Distribution Plate 3 Well 17G
type: Collection
persistent identity: iGEM 2018 Plate3 Well17G
display id: iGEM_2018_Plate3_Well17G
version: 1
owned by: myers
member: ecfp

iGEM 2018 Distribution Plate 3 Well 4J
type: Collection
persistent identity: iGEM 2018 Plate3 Well4J
display id: iGEM_2018_Plate3_Well4J
version: 1
owned by: myers
member: BBa_J52019

iGEM 2018 Distribution Plate 3 Well 22D
type: Collection
persistent identity: iGEM 2018 Plate3 Well22D
display id: iGEM_2018_Plate3_Well22D
version: 1
owned by: myers
member: BBa_I13533

iGEM 2018 Distribution Plate 3 Well 21O
type: Collection
persistent identity: iGEM 2018 Plate3 Well21O
display id: iGEM_2018_Plate3_Well21O
version: 1
owned by: myers
member: OriTR

iGEM 2018 Distribution Plate 3 Well 13C
type: Collection
persistent identity: iGEM 2018 Plate3 Well13C
display id: iGEM_2018_Plate3_Well13C
version: 1
owned by: myers
member: PT7-E

iGEM 2018 Distribution Plate 3 Well 13P
type: Collection
persistent identity: iGEM 2018 Plate3 Well13P
display id: iGEM_2018_Plate3_Well13P
version: 1
owned by: myers
member: BBa_J06801

iGEM 2018 Distribution Plate 3 Well 14F
type: Collection
persistent identity: iGEM 2018 Plate3 Well14F
display id: iGEM_2018_Plate3_Well14F
version: 1
owned by: myers
member: BBa_I13606

iGEM 2018 Distribution Plate 3 Well 10F
type: Collection
persistent identity: iGEM 2018 Plate3 Well10F
display id: iGEM_2018_Plate3_Well10F
version: 1
owned by: myers
member: BBa_K200028

iGEM 2018 Distribution Plate 3 Well 19G
type: Collection
persistent identity: iGEM 2018 Plate3 Well19G
display id: iGEM_2018_Plate3_Well19G
version: 1
owned by: myers
member: BBa_I0466

iGEM 2018 Distribution Plate 3 Well 20C
type: Collection
persistent identity: iGEM 2018 Plate3 Well20C
display id: iGEM_2018_Plate3_Well20C
version: 1
owned by: myers
member: BBa_K118011

iGEM 2018 Distribution Plate 3 Well 3H
type: Collection
persistent identity: iGEM 2018 Plate3 Well3H
display id: iGEM_2018_Plate3_Well3H
version: 1
owned by: myers
member: BBa_J52028

iGEM 2018 Distribution Plate 3 Well 6E
type: Collection
persistent identity: iGEM 2018 Plate3 Well6E
display id: iGEM_2018_Plate3_Well6E
version: 1
owned by: myers
member: BBa_B0021

iGEM 2018 Distribution Plate 3 Well 4P
type: Collection
persistent identity: iGEM 2018 Plate3 Well4P
display id: iGEM_2018_Plate3_Well4P
version: 1
owned by: myers
member: BBa_S03650

iGEM 2018 Distribution Plate 3 Well 3L
type: Collection
persistent identity: iGEM 2018 Plate3 Well3L
display id: iGEM_2018_Plate3_Well3L
version: 1
owned by: myers
member: PelB

iGEM 2018 Distribution Plate 3 Well 13I
type: Collection
persistent identity: iGEM 2018 Plate3 Well13I
display id: iGEM_2018_Plate3_Well13I
version: 1
owned by: myers
member: BBa_I718012

iGEM 2018 Distribution Plate 3 Well 11H
type: Collection
persistent identity: iGEM 2018 Plate3 Well11H
display id: iGEM_2018_Plate3_Well11H
version: 1
owned by: myers
member: AraC O2

iGEM 2018 Distribution Plate 3 Well 15C
type: Collection
persistent identity: iGEM 2018 Plate3 Well15C
display id: iGEM_2018_Plate3_Well15C
version: 1
owned by: myers
member: BBa_S03857

iGEM 2018 Distribution Plate 3 Well 5H
type: Collection
persistent identity: iGEM 2018 Plate3 Well5H
display id: iGEM_2018_Plate3_Well5H
version: 1
owned by: myers
member: BBa_B1008

iGEM 2018 Distribution Plate 3 Well 16C
type: Collection
persistent identity: iGEM 2018 Plate3 Well16C
display id: iGEM_2018_Plate3_Well16C
version: 1
owned by: myers
member: BBa_K091146

iGEM 2018 Distribution Plate 3 Well 7D
type: Collection
persistent identity: iGEM 2018 Plate3 Well7D
display id: iGEM_2018_Plate3_Well7D
version: 1
owned by: myers
member: BBa_J52012

iGEM 2018 Distribution Plate 3 Well 12C
type: Collection
persistent identity: iGEM 2018 Plate3 Well12C
display id: iGEM_2018_Plate3_Well12C
version: 1
owned by: myers
member: BBa_E0432

iGEM 2018 Distribution Plate 3 Well 20N
type: Collection
persistent identity: iGEM 2018 Plate3 Well20N
display id: iGEM_2018_Plate3_Well20N
version: 1
owned by: myers
member: BBa_I15018

iGEM 2018 Distribution Plate 3 Well 7L
type: Collection
persistent identity: iGEM 2018 Plate3 Well7L
display id: iGEM_2018_Plate3_Well7L
version: 1
owned by: myers
member: mCherry

iGEM 2018 Distribution Plate 3 Well 16P
type: Collection
persistent identity: iGEM 2018 Plate3 Well16P
display id: iGEM_2018_Plate3_Well16P
version: 1
owned by: myers
member: BBa_I715040

iGEM 2018 Distribution Plate 3 Well 2O
type: Collection
persistent identity: iGEM 2018 Plate3 Well2O
display id: iGEM_2018_Plate3_Well2O
version: 1
owned by: myers
member: BBa_C2006

iGEM 2018 Distribution Plate 3 Well 13A
type: Collection
persistent identity: iGEM 2018 Plate3 Well13A
display id: iGEM_2018_Plate3_Well13A
version: 1
owned by: myers
member: PT7-D

iGEM 2018 Distribution Plate 3 Well 21B
type: Collection
persistent identity: iGEM 2018 Plate3 Well21B
display id: iGEM_2018_Plate3_Well21B
version: 1
owned by: myers
member: BBa_K081015

iGEM 2018 Distribution Plate 3 Well 18H
type: Collection
persistent identity: iGEM 2018 Plate3 Well18H
display id: iGEM_2018_Plate3_Well18H
version: 1
owned by: myers
member: BBa_I746382

iGEM 2018 Distribution Plate 3 Well 20J
type: Collection
persistent identity: iGEM 2018 Plate3 Well20J
display id: iGEM_2018_Plate3_Well20J
version: 1
owned by: myers
member: BBa_J24018

iGEM 2018 Distribution Plate 3 Well 17E
type: Collection
persistent identity: iGEM 2018 Plate3 Well17E
display id: iGEM_2018_Plate3_Well17E
version: 1
owned by: myers
member: ecfp

iGEM 2018 Distribution Plate 3 Well 19A
type: Collection
persistent identity: iGEM 2018 Plate3 Well19A
display id: iGEM_2018_Plate3_Well19A
version: 1
owned by: myers
member: BBa_I13002

iGEM 2018 Distribution Plate 3 Well 11C
type: Collection
persistent identity: iGEM 2018 Plate3 Well11C
display id: iGEM_2018_Plate3_Well11C
version: 1
owned by: myers
member: BBa_I714031

iGEM 2018 Distribution Plate 3 Well 19D
type: Collection
persistent identity: iGEM 2018 Plate3 Well19D
display id: iGEM_2018_Plate3_Well19D
version: 1
owned by: myers
member: mRFP1

iGEM 2018 Distribution Plate 3 Well 6D
type: Collection
persistent identity: iGEM 2018 Plate3 Well6D
display id: iGEM_2018_Plate3_Well6D
version: 1
owned by: myers
member: BBa_J04771

iGEM 2018 Distribution Plate 3 Well 18O
type: Collection
persistent identity: iGEM 2018 Plate3 Well18O
display id: iGEM_2018_Plate3_Well18O
version: 1
owned by: myers
member: BBa_K118006

iGEM 2018 Distribution Plate 3 Well 9H
type: Collection
persistent identity: iGEM 2018 Plate3 Well9H
display id: iGEM_2018_Plate3_Well9H
version: 1
owned by: myers
member: cI TP901

iGEM 2018 Distribution Plate 3 Well 8L
type: Collection
persistent identity: iGEM 2018 Plate3 Well8L
display id: iGEM_2018_Plate3_Well8L
version: 1
owned by: myers
member: cI 434

iGEM 2018 Distribution Plate 3 Well 15G
type: Collection
persistent identity: iGEM 2018 Plate3 Well15G
display id: iGEM_2018_Plate3_Well15G
version: 1
owned by: myers
member: BBa_S03859

iGEM 2018 Distribution Plate 3 Well 13M
type: Collection
persistent identity: iGEM 2018 Plate3 Well13M
display id: iGEM_2018_Plate3_Well13M
version: 1
owned by: myers
member: BBa_I718014

iGEM 2018 Distribution Plate 3 Well 13N
type: Collection
persistent identity: iGEM 2018 Plate3 Well13N
display id: iGEM_2018_Plate3_Well13N
version: 1
owned by: myers
member: BBa_J06800

iGEM 2018 Distribution Plate 3 Well 5J
type: Collection
persistent identity: iGEM 2018 Plate3 Well5J
display id: iGEM_2018_Plate3_Well5J
version: 1
owned by: myers
member: BBa_B1009

iGEM 2018 Distribution Plate 3 Well 10A
type: Collection
persistent identity: iGEM 2018 Plate3 Well10A
display id: iGEM_2018_Plate3_Well10A
version: 1
owned by: myers
member: BBa_K203109

iGEM 2018 Distribution Plate 3 Well 12A
type: Collection
persistent identity: iGEM 2018 Plate3 Well12A
display id: iGEM_2018_Plate3_Well12A
version: 1
owned by: myers
member: BBa_I714075

iGEM 2018 Distribution Plate 3 Well 20L
type: Collection
persistent identity: iGEM 2018 Plate3 Well20L
display id: iGEM_2018_Plate3_Well20L
version: 1
owned by: myers
member: ATF1

iGEM 2018 Distribution Plate 3 Well 5G
type: Collection
persistent identity: iGEM 2018 Plate3 Well5G
display id: iGEM_2018_Plate3_Well5G
version: 1
owned by: myers
member: AraC

iGEM 2018 Distribution Plate 3 Well 14I
type: Collection
persistent identity: iGEM 2018 Plate3 Well14I
display id: iGEM_2018_Plate3_Well14I
version: 1
owned by: myers
member: BBa_K145215

iGEM 2018 Distribution Plate 3 Well 24O
type: Collection
persistent identity: iGEM 2018 Plate3 Well24O
display id: iGEM_2018_Plate3_Well24O
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 3 Well 16M
type: Collection
persistent identity: iGEM 2018 Plate3 Well16M
display id: iGEM_2018_Plate3_Well16M
version: 1
owned by: myers
member: BBa_K081013

iGEM 2018 Distribution Plate 3 Well 22G
type: Collection
persistent identity: iGEM 2018 Plate3 Well22G
display id: iGEM_2018_Plate3_Well22G
version: 1
owned by: myers
member: BBa_K137043

iGEM 2018 Distribution Plate 3 Well 6N
type: Collection
persistent identity: iGEM 2018 Plate3 Well6N
display id: iGEM_2018_Plate3_Well6N
version: 1
owned by: myers
member: BBa_I732830

iGEM 2018 Distribution Plate 3 Well 12B
type: Collection
persistent identity: iGEM 2018 Plate3 Well12B
display id: iGEM_2018_Plate3_Well12B
version: 1
owned by: myers
member: BBa_S01009

iGEM 2018 Distribution Plate 3 Well 4L
type: Collection
persistent identity: iGEM 2018 Plate3 Well4L
display id: iGEM_2018_Plate3_Well4L
version: 1
owned by: myers
member: BBa_J45397

iGEM 2018 Distribution Plate 3 Well 3F
type: Collection
persistent identity: iGEM 2018 Plate3 Well3F
display id: iGEM_2018_Plate3_Well3F
version: 1
owned by: myers
member: BBa_B0015

iGEM 2018 Distribution Plate 3 Well 12N
type: Collection
persistent identity: iGEM 2018 Plate3 Well12N
display id: iGEM_2018_Plate3_Well12N
version: 1
owned by: myers
member: BBa_E0615

iGEM 2018 Distribution Plate 3 Well 1J
type: Collection
persistent identity: iGEM 2018 Plate3 Well1J
display id: iGEM_2018_Plate3_Well1J
version: 1
owned by: myers
member: BBa_C0056

iGEM 2018 Distribution Plate 3 Well 3I
type: Collection
persistent identity: iGEM 2018 Plate3 Well3I
display id: iGEM_2018_Plate3_Well3I
version: 1
owned by: myers
member: OmpR

iGEM 2018 Distribution Plate 3 Well 5A
type: Collection
persistent identity: iGEM 2018 Plate3 Well5A
display id: iGEM_2018_Plate3_Well5A
version: 1
owned by: myers
member: BBa_S03119

iGEM 2018 Distribution Plate 3 Well 3M
type: Collection
persistent identity: iGEM 2018 Plate3 Well3M
display id: iGEM_2018_Plate3_Well3M
version: 1
owned by: myers
member: cinR

iGEM 2018 Distribution Plate 3 Well 16B
type: Collection
persistent identity: iGEM 2018 Plate3 Well16B
display id: iGEM_2018_Plate3_Well16B
version: 1
owned by: myers
member: BBa_I13515

iGEM 2018 Distribution Plate 3 Well 21L
type: Collection
persistent identity: iGEM 2018 Plate3 Well21L
display id: iGEM_2018_Plate3_Well21L
version: 1
owned by: myers
member: BBa_K081012

iGEM 2018 Distribution Plate 3 Well 7C
type: Collection
persistent identity: iGEM 2018 Plate3 Well7C
display id: iGEM_2018_Plate3_Well7C
version: 1
owned by: myers
member: BBa_I13602

iGEM 2018 Distribution Plate 3 Well 24P
type: Collection
persistent identity: iGEM 2018 Plate3 Well24P
display id: iGEM_2018_Plate3_Well24P
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 3 Well 4N
type: Collection
persistent identity: iGEM 2018 Plate3 Well4N
display id: iGEM_2018_Plate3_Well4N
version: 1
owned by: myers
member: BBa_S03591

iGEM 2018 Distribution Plate 3 Well 16L
type: Collection
persistent identity: iGEM 2018 Plate3 Well16L
display id: iGEM_2018_Plate3_Well16L
version: 1
owned by: myers
member: BBa_I15015

iGEM 2018 Distribution Plate 3 Well 1L
type: Collection
persistent identity: iGEM 2018 Plate3 Well1L
display id: iGEM_2018_Plate3_Well1L
version: 1
owned by: myers
member: BBa_I1466

iGEM 2018 Distribution Plate 3 Well 16G
type: Collection
persistent identity: iGEM 2018 Plate3 Well16G
display id: iGEM_2018_Plate3_Well16G
version: 1
owned by: myers
member: BBa_K081007

iGEM 2018 Distribution Plate 3 Well 8O
type: Collection
persistent identity: iGEM 2018 Plate3 Well8O
display id: iGEM_2018_Plate3_Well8O
version: 1
owned by: myers
member: BBa_J37032

iGEM 2018 Distribution Plate 3 Well 5B
type: Collection
persistent identity: iGEM 2018 Plate3 Well5B
display id: iGEM_2018_Plate3_Well5B
version: 1
owned by: myers
member: BBa_B1005

iGEM 2018 Distribution Plate 3 Well 17K
type: Collection
persistent identity: iGEM 2018 Plate3 Well17K
display id: iGEM_2018_Plate3_Well17K
version: 1
owned by: myers
member: BBa_I13001

iGEM 2018 Distribution Plate 3 Well 19O
type: Collection
persistent identity: iGEM 2018 Plate3 Well19O
display id: iGEM_2018_Plate3_Well19O
version: 1
owned by: myers
member: BBa_I13453

iGEM 2018 Distribution Plate 3 Well 23A
type: Collection
persistent identity: iGEM 2018 Plate3 Well23A
display id: iGEM_2018_Plate3_Well23A
version: 1
owned by: myers
member: Adapter

iGEM 2018 Distribution Plate 3 Well 8M
type: Collection
persistent identity: iGEM 2018 Plate3 Well8M
display id: iGEM_2018_Plate3_Well8M
version: 1
owned by: myers
member: BBa_I746909

iGEM 2018 Distribution Plate 3 Well 1A
type: Collection
persistent identity: iGEM 2018 Plate3 Well1A
display id: iGEM_2018_Plate3_Well1A
version: 1
owned by: myers
member: BBa_S03156

iGEM 2018 Distribution Plate 3 Well 4H
type: Collection
persistent identity: iGEM 2018 Plate3 Well4H
display id: iGEM_2018_Plate3_Well4H
version: 1
owned by: myers
member: BBa_I1468

iGEM 2018 Distribution Plate 3 Well 21C
type: Collection
persistent identity: iGEM 2018 Plate3 Well21C
display id: iGEM_2018_Plate3_Well21C
version: 1
owned by: myers
member: BBa_J44001

iGEM 2018 Distribution Plate 3 Well 20P
type: Collection
persistent identity: iGEM 2018 Plate3 Well20P
display id: iGEM_2018_Plate3_Well20P
version: 1
owned by: myers
member: BBa_I13537

iGEM 2018 Distribution Plate 3 Well 9L
type: Collection
persistent identity: iGEM 2018 Plate3 Well9L
display id: iGEM_2018_Plate3_Well9L
version: 1
owned by: myers
member: cinr

iGEM 2018 Distribution Plate 3 Well 15J
type: Collection
persistent identity: iGEM 2018 Plate3 Well15J
display id: iGEM_2018_Plate3_Well15J
version: 1
owned by: myers
member: BBa_P0340

iGEM 2018 Distribution Plate 3 Well 15K
type: Collection
persistent identity: iGEM 2018 Plate3 Well15K
display id: iGEM_2018_Plate3_Well15K
version: 1
owned by: myers
member: BBa_S03877

iGEM 2018 Distribution Plate 3 Well 7J
type: Collection
persistent identity: iGEM 2018 Plate3 Well7J
display id: iGEM_2018_Plate3_Well7J
version: 1
owned by: myers
member: BBa_E2030

iGEM 2018 Distribution Plate 3 Well 13B
type: Collection
persistent identity: iGEM 2018 Plate3 Well13B
display id: iGEM_2018_Plate3_Well13B
version: 1
owned by: myers
member: RhI

iGEM 2018 Distribution Plate 3 Well 23C
type: Collection
persistent identity: iGEM 2018 Plate3 Well23C
display id: iGEM_2018_Plate3_Well23C
version: 1
owned by: myers
member: LRLa

iGEM 2018 Distribution Plate 3 Well 3K
type: Collection
persistent identity: iGEM 2018 Plate3 Well3K
display id: iGEM_2018_Plate3_Well3K
version: 1
owned by: myers
member: OmpR

iGEM 2018 Distribution Plate 3 Well 6A
type: Collection
persistent identity: iGEM 2018 Plate3 Well6A
display id: iGEM_2018_Plate3_Well6A
version: 1
owned by: myers
member: BBa_I13973

iGEM 2018 Distribution Plate 3 Well 17I
type: Collection
persistent identity: iGEM 2018 Plate3 Well17I
display id: iGEM_2018_Plate3_Well17I
version: 1
owned by: myers
member: spacer

iGEM 2018 Distribution Plate 3 Well 2P
type: Collection
persistent identity: iGEM 2018 Plate3 Well2P
display id: iGEM_2018_Plate3_Well2P
version: 1
owned by: myers
member: BBa_S03724

iGEM 2018 Distribution Plate 3 Well 21G
type: Collection
persistent identity: iGEM 2018 Plate3 Well21G
display id: iGEM_2018_Plate3_Well21G
version: 1
owned by: myers
member: BBa_S03518

iGEM 2018 Distribution Plate 3 Well 11E
type: Collection
persistent identity: iGEM 2018 Plate3 Well11E
display id: iGEM_2018_Plate3_Well11E
version: 1
owned by: myers
member: BBa_I714044

iGEM 2018 Distribution Plate 3 Well 15I
type: Collection
persistent identity: iGEM 2018 Plate3 Well15I
display id: iGEM_2018_Plate3_Well15I
version: 1
owned by: myers
member: rbs+COMT

iGEM 2018 Distribution Plate 3 Well 2F
type: Collection
persistent identity: iGEM 2018 Plate3 Well2F
display id: iGEM_2018_Plate3_Well2F
version: 1
owned by: myers
member: BBa_I732020

iGEM 2018 Distribution Plate 3 Well 23E
type: Collection
persistent identity: iGEM 2018 Plate3 Well23E
display id: iGEM_2018_Plate3_Well23E
version: 1
owned by: myers
member: RFP1

iGEM 2018 Distribution Plate 3 Well 15E
type: Collection
persistent identity: iGEM 2018 Plate3 Well15E
display id: iGEM_2018_Plate3_Well15E
version: 1
owned by: myers
member: BBa_S03858

iGEM 2018 Distribution Plate 3 Well 13K
type: Collection
persistent identity: iGEM 2018 Plate3 Well13K
display id: iGEM_2018_Plate3_Well13K
version: 1
owned by: myers
member: BBa_I718013

iGEM 2018 Distribution Plate 3 Well 15A
type: Collection
persistent identity: iGEM 2018 Plate3 Well15A
display id: iGEM_2018_Plate3_Well15A
version: 1
owned by: myers
member: BBa_S03855

iGEM 2018 Distribution Plate 3 Well 3N
type: Collection
persistent identity: iGEM 2018 Plate3 Well3N
display id: iGEM_2018_Plate3_Well3N
version: 1
owned by: myers
member: BBa_S03506

iGEM 2018 Distribution Plate 3 Well 18P
type: Collection
persistent identity: iGEM 2018 Plate3 Well18P
display id: iGEM_2018_Plate3_Well18P
version: 1
owned by: myers
member: BBa_I746395

iGEM 2018 Distribution Plate 3 Well 22J
type: Collection
persistent identity: iGEM 2018 Plate3 Well22J
display id: iGEM_2018_Plate3_Well22J
version: 1
owned by: myers
member: BBa_T9002

iGEM 2018 Distribution Plate 3 Well 1B
type: Collection
persistent identity: iGEM 2018 Plate3 Well1B
display id: iGEM_2018_Plate3_Well1B
version: 1
owned by: myers
member: BBa_K118003

iGEM 2018 Distribution Plate 3 Well 10E
type: Collection
persistent identity: iGEM 2018 Plate3 Well10E
display id: iGEM_2018_Plate3_Well10E
version: 1
owned by: myers
member: BBa_K203111

iGEM 2018 Distribution Plate 3 Well 13F
type: Collection
persistent identity: iGEM 2018 Plate3 Well13F
display id: iGEM_2018_Plate3_Well13F
version: 1
owned by: myers
member: BBa_I12036

iGEM 2018 Distribution Plate 3 Well 18E
type: Collection
persistent identity: iGEM 2018 Plate3 Well18E
display id: iGEM_2018_Plate3_Well18E
version: 1
owned by: myers
member: BBa_K081024

iGEM 2018 Distribution Plate 3 Well 7N
type: Collection
persistent identity: iGEM 2018 Plate3 Well7N
display id: iGEM_2018_Plate3_Well7N
version: 1
owned by: myers
member: rhII

iGEM 2018 Distribution Plate 3 Well 20D
type: Collection
persistent identity: iGEM 2018 Plate3 Well20D
display id: iGEM_2018_Plate3_Well20D
version: 1
owned by: myers
member: BBa_S03827

iGEM 2018 Distribution Plate 3 Well 17B
type: Collection
persistent identity: iGEM 2018 Plate3 Well17B
display id: iGEM_2018_Plate3_Well17B
version: 1
owned by: myers
member: Prm +

iGEM 2018 Distribution Plate 3 Well 11F
type: Collection
persistent identity: iGEM 2018 Plate3 Well11F
display id: iGEM_2018_Plate3_Well11F
version: 1
owned by: myers
member: Mnt

iGEM 2018 Distribution Plate 3 Well 9G
type: Collection
persistent identity: iGEM 2018 Plate3 Well9G
display id: iGEM_2018_Plate3_Well9G
version: 1
owned by: myers
member: BBa_I732801

iGEM 2018 Distribution Plate 3 Well 3A
type: Collection
persistent identity: iGEM 2018 Plate3 Well3A
display id: iGEM_2018_Plate3_Well3A
version: 1
owned by: myers
member: BBa_E5504

iGEM 2018 Distribution Plate 3 Well 8C
type: Collection
persistent identity: iGEM 2018 Plate3 Well8C
display id: iGEM_2018_Plate3_Well8C
version: 1
owned by: myers
member: GFP2

iGEM 2018 Distribution Plate 3 Well 2L
type: Collection
persistent identity: iGEM 2018 Plate3 Well2L
display id: iGEM_2018_Plate3_Well2L
version: 1
owned by: myers
member: Bla

iGEM 2018 Distribution Plate 3 Well 17M
type: Collection
persistent identity: iGEM 2018 Plate3 Well17M
display id: iGEM_2018_Plate3_Well17M
version: 1
owned by: myers
member: R6K

iGEM 2018 Distribution Plate 3 Well 19L
type: Collection
persistent identity: iGEM 2018 Plate3 Well19L
display id: iGEM_2018_Plate3_Well19L
version: 1
owned by: myers
member: mRFP1

iGEM 2018 Distribution Plate 3 Well 24H
type: Collection
persistent identity: iGEM 2018 Plate3 Well24H
display id: iGEM_2018_Plate3_Well24H
version: 1
owned by: myers
member: BBa_J24676

iGEM 2018 Distribution Plate 3 Well 2G
type: Collection
persistent identity: iGEM 2018 Plate3 Well2G
display id: iGEM_2018_Plate3_Well2G
version: 1
owned by: myers
member: difR

iGEM 2018 Distribution Plate 3 Well 12L
type: Collection
persistent identity: iGEM 2018 Plate3 Well12L
display id: iGEM_2018_Plate3_Well12L
version: 1
owned by: myers
member: BBa_I0407

iGEM 2018 Distribution Plate 3 Well 6H
type: Collection
persistent identity: iGEM 2018 Plate3 Well6H
display id: iGEM_2018_Plate3_Well6H
version: 1
owned by: myers
member: BBa_J04971

iGEM 2018 Distribution Plate 3 Well 6B
type: Collection
persistent identity: iGEM 2018 Plate3 Well6B
display id: iGEM_2018_Plate3_Well6B
version: 1
owned by: myers
member: BBa_S03653

iGEM 2018 Distribution Plate 3 Well 19F
type: Collection
persistent identity: iGEM 2018 Plate3 Well19F
display id: iGEM_2018_Plate3_Well19F
version: 1
owned by: myers
member: BBa_M0100

iGEM 2018 Distribution Plate 3 Well 1G
type: Collection
persistent identity: iGEM 2018 Plate3 Well1G
display id: iGEM_2018_Plate3_Well1G
version: 1
owned by: myers
member: ECFP

iGEM 2018 Distribution Plate 3 Well 16J
type: Collection
persistent identity: iGEM 2018 Plate3 Well16J
display id: iGEM_2018_Plate3_Well16J
version: 1
owned by: myers
member: BBa_I13977

iGEM 2018 Distribution Plate 3 Well 22F
type: Collection
persistent identity: iGEM 2018 Plate3 Well22F
display id: iGEM_2018_Plate3_Well22F
version: 1
owned by: myers
member: BBa_I13539

iGEM 2018 Distribution Plate 3 Well 15P
type: Collection
persistent identity: iGEM 2018 Plate3 Well15P
display id: iGEM_2018_Plate3_Well15P
version: 1
owned by: myers
member: BBa_P0453

iGEM 2018 Distribution Plate 3 Well 17L
type: Collection
persistent identity: iGEM 2018 Plate3 Well17L
display id: iGEM_2018_Plate3_Well17L
version: 1
owned by: myers
member: P22 attB

iGEM 2018 Distribution Plate 3 Well 22M
type: Collection
persistent identity: iGEM 2018 Plate3 Well22M
display id: iGEM_2018_Plate3_Well22M
version: 1
owned by: myers
member: BBa_K105024

iGEM 2018 Distribution Plate 3 Well 8F
type: Collection
persistent identity: iGEM 2018 Plate3 Well8F
display id: iGEM_2018_Plate3_Well8F
version: 1
owned by: myers
member: ARL2A0103

iGEM 2018 Distribution Plate 3 Well 7P
type: Collection
persistent identity: iGEM 2018 Plate3 Well7P
display id: iGEM_2018_Plate3_Well7P
version: 1
owned by: myers
member: rhlR

iGEM 2018 Distribution Plate 3 Well 12J
type: Collection
persistent identity: iGEM 2018 Plate3 Well12J
display id: iGEM_2018_Plate3_Well12J
version: 1
owned by: myers
member: BBa_I0406

iGEM 2018 Distribution Plate 3 Well 6M
type: Collection
persistent identity: iGEM 2018 Plate3 Well6M
display id: iGEM_2018_Plate3_Well6M
version: 1
owned by: myers
member: lasR

iGEM 2018 Distribution Plate 3 Well 10I
type: Collection
persistent identity: iGEM 2018 Plate3 Well10I
display id: iGEM_2018_Plate3_Well10I
version: 1
owned by: myers
member: BBa_K203125

iGEM 2018 Distribution Plate 3 Well 19B
type: Collection
persistent identity: iGEM 2018 Plate3 Well19B
display id: iGEM_2018_Plate3_Well19B
version: 1
owned by: myers
member: BBa_I11031

iGEM 2018 Distribution Plate 3 Well 4B
type: Collection
persistent identity: iGEM 2018 Plate3 Well4B
display id: iGEM_2018_Plate3_Well4B
version: 1
owned by: myers
member: BBa_S03881

iGEM 2018 Distribution Plate 3 Well 2C
type: Collection
persistent identity: iGEM 2018 Plate3 Well2C
display id: iGEM_2018_Plate3_Well2C
version: 1
owned by: myers
member: lox71

iGEM 2018 Distribution Plate 3 Well 20B
type: Collection
persistent identity: iGEM 2018 Plate3 Well20B
display id: iGEM_2018_Plate3_Well20B
version: 1
owned by: myers
member: BBa_I746009

iGEM 2018 Distribution Plate 3 Well 21N
type: Collection
persistent identity: iGEM 2018 Plate3 Well21N
display id: iGEM_2018_Plate3_Well21N
version: 1
owned by: myers
member: BBa_K081014

iGEM 2018 Distribution Plate 3 Well 4I
type: Collection
persistent identity: iGEM 2018 Plate3 Well4I
display id: iGEM_2018_Plate3_Well4I
version: 1
owned by: myers
member: GFP1

iGEM 2018 Distribution Plate 3 Well 3P
type: Collection
persistent identity: iGEM 2018 Plate3 Well3P
display id: iGEM_2018_Plate3_Well3P
version: 1
owned by: myers
member: BBa_B1003

iGEM 2018 Distribution Plate 3 Well 5E
type: Collection
persistent identity: iGEM 2018 Plate3 Well5E
display id: iGEM_2018_Plate3_Well5E
version: 1
owned by: myers
member: RhlR+C4

iGEM 2018 Distribution Plate 3 Well 12E
type: Collection
persistent identity: iGEM 2018 Plate3 Well12E
display id: iGEM_2018_Plate3_Well12E
version: 1
owned by: myers
member: mCherry

iGEM 2018 Distribution Plate 3 Well 23K
type: Collection
persistent identity: iGEM 2018 Plate3 Well23K
display id: iGEM_2018_Plate3_Well23K
version: 1
owned by: myers
member: pLambda+RF

iGEM 2018 Distribution Plate 3 Well 10C
type: Collection
persistent identity: iGEM 2018 Plate3 Well10C
display id: iGEM_2018_Plate3_Well10C
version: 1
owned by: myers
member: BBa_K203110

iGEM 2018 Distribution Plate 3 Well 5L
type: Collection
persistent identity: iGEM 2018 Plate3 Well5L
display id: iGEM_2018_Plate3_Well5L
version: 1
owned by: myers
member: BBa_B1010

iGEM 2018 Distribution Plate 3 Well 14H
type: Collection
persistent identity: iGEM 2018 Plate3 Well14H
display id: iGEM_2018_Plate3_Well14H
version: 1
owned by: myers
member: BBa_I13604

iGEM 2018 Distribution Plate 3 Well 9N
type: Collection
persistent identity: iGEM 2018 Plate3 Well9N
display id: iGEM_2018_Plate3_Well9N
version: 1
owned by: myers
member: lasI

iGEM 2018 Distribution Plate 3 Well 11D
type: Collection
persistent identity: iGEM 2018 Plate3 Well11D
display id: iGEM_2018_Plate3_Well11D
version: 1
owned by: myers
member: luxR

iGEM 2018 Distribution Plate 3 Well 19P
type: Collection
persistent identity: iGEM 2018 Plate3 Well19P
display id: iGEM_2018_Plate3_Well19P
version: 1
owned by: myers
member: BBa_K082006

iGEM 2018 Distribution Plate 3 Well 6O
type: Collection
persistent identity: iGEM 2018 Plate3 Well6O
display id: iGEM_2018_Plate3_Well6O
version: 1
owned by: myers
member: luxI

iGEM 2018 Distribution Plate 3 Well 22K
type: Collection
persistent identity: iGEM 2018 Plate3 Well22K
display id: iGEM_2018_Plate3_Well22K
version: 1
owned by: myers
member: BBa_K105022

iGEM 2018 Distribution Plate 3 Well 8K
type: Collection
persistent identity: iGEM 2018 Plate3 Well8K
display id: iGEM_2018_Plate3_Well8K
version: 1
owned by: myers
member: BBa_I750013

iGEM 2018 Distribution Plate 3 Well 4M
type: Collection
persistent identity: iGEM 2018 Plate3 Well4M
display id: iGEM_2018_Plate3_Well4M
version: 1
owned by: myers
member: BBa_J33203

iGEM 2018 Distribution Plate 3 Well 18G
type: Collection
persistent identity: iGEM 2018 Plate3 Well18G
display id: iGEM_2018_Plate3_Well18G
version: 1
owned by: myers
member: BBa_K117000

iGEM 2018 Distribution Plate 3 Well 9A
type: Collection
persistent identity: iGEM 2018 Plate3 Well9A
display id: iGEM_2018_Plate3_Well9A
version: 1
owned by: myers
member: BBa_I732912

iGEM 2018 Distribution Plate 3 Well 6F
type: Collection
persistent identity: iGEM 2018 Plate3 Well6F
display id: iGEM_2018_Plate3_Well6F
version: 1
owned by: myers
member: BBa_J04871

iGEM 2018 Distribution Plate 3 Well 15L
type: Collection
persistent identity: iGEM 2018 Plate3 Well15L
display id: iGEM_2018_Plate3_Well15L
version: 1
owned by: myers
member: BBa_P0353

iGEM 2018 Distribution Plate 3 Well 20O
type: Collection
persistent identity: iGEM 2018 Plate3 Well20O
display id: iGEM_2018_Plate3_Well20O
version: 1
owned by: myers
member: BBa_K082034

iGEM 2018 Distribution Plate 3 Well 2E
type: Collection
persistent identity: iGEM 2018 Plate3 Well2E
display id: iGEM_2018_Plate3_Well2E
version: 1
owned by: myers
member: difF

iGEM 2018 Distribution Plate 3 Well 13G
type: Collection
persistent identity: iGEM 2018 Plate3 Well13G
display id: iGEM_2018_Plate3_Well13G
version: 1
owned by: myers
member: BBa_I718011

iGEM 2018 Distribution Plate 3 Well 11A
type: Collection
persistent identity: iGEM 2018 Plate3 Well11A
display id: iGEM_2018_Plate3_Well11A
version: 1
owned by: myers
member: BBa_I715032

iGEM 2018 Distribution Plate 3 Well 1I
type: Collection
persistent identity: iGEM 2018 Plate3 Well1I
display id: iGEM_2018_Plate3_Well1I
version: 1
owned by: myers
member: EYFP

iGEM 2018 Distribution Plate 3 Well 19E
type: Collection
persistent identity: iGEM 2018 Plate3 Well19E
display id: iGEM_2018_Plate3_Well19E
version: 1
owned by: myers
member: BBa_S03154

iGEM 2018 Distribution Plate 3 Well 4K
type: Collection
persistent identity: iGEM 2018 Plate3 Well4K
display id: iGEM_2018_Plate3_Well4K
version: 1
owned by: myers
member: lox

iGEM 2018 Distribution Plate 3 Well 10M
type: Collection
persistent identity: iGEM 2018 Plate3 Well10M
display id: iGEM_2018_Plate3_Well10M
version: 1
owned by: myers
member: BBa_J33202

iGEM 2018 Distribution Plate 3 Well 14D
type: Collection
persistent identity: iGEM 2018 Plate3 Well14D
display id: iGEM_2018_Plate3_Well14D
version: 1
owned by: myers
member: BBa_I13263

iGEM 2018 Distribution Plate 3 Well 14L
type: Collection
persistent identity: iGEM 2018 Plate3 Well14L
display id: iGEM_2018_Plate3_Well14L
version: 1
owned by: myers
member: BBa_I13404

iGEM 2018 Distribution Plate 3 Well 12P
type: Collection
persistent identity: iGEM 2018 Plate3 Well12P
display id: iGEM_2018_Plate3_Well12P
version: 1
owned by: myers
member: BBa_I13023

iGEM 2018 Distribution Plate 3 Well 14J
type: Collection
persistent identity: iGEM 2018 Plate3 Well14J
display id: iGEM_2018_Plate3_Well14J
version: 1
owned by: myers
member: BBa_I13605

iGEM 2018 Distribution Plate 3 Well 18N
type: Collection
persistent identity: iGEM 2018 Plate3 Well18N
display id: iGEM_2018_Plate3_Well18N
version: 1
owned by: myers
member: BBa_I746394

iGEM 2018 Distribution Plate 3 Well 24K
type: Collection
persistent identity: iGEM 2018 Plate3 Well24K
display id: iGEM_2018_Plate3_Well24K
version: 1
owned by: myers
member: BBa_K081020

iGEM 2018 Distribution Plate 3 Well 15B
type: Collection
persistent identity: iGEM 2018 Plate3 Well15B
display id: iGEM_2018_Plate3_Well15B
version: 1
owned by: myers
member: mCherry

iGEM 2018 Distribution Plate 3 Well 9P
type: Collection
persistent identity: iGEM 2018 Plate3 Well9P
display id: iGEM_2018_Plate3_Well9P
version: 1
owned by: myers
member: lasr

iGEM 2018 Distribution Plate 3 Well 23D
type: Collection
persistent identity: iGEM 2018 Plate3 Well23D
display id: iGEM_2018_Plate3_Well23D
version: 1
owned by: myers
member: BBa_K137055

iGEM 2018 Distribution Plate 3 Well 8N
type: Collection
persistent identity: iGEM 2018 Plate3 Well8N
display id: iGEM_2018_Plate3_Well8N
version: 1
owned by: myers
member: cI HK022

iGEM 2018 Distribution Plate 3 Well 12I
type: Collection
persistent identity: iGEM 2018 Plate3 Well12I
display id: iGEM_2018_Plate3_Well12I
version: 1
owned by: myers
member: BBa_I746907

iGEM 2018 Distribution Plate 3 Well 14M
type: Collection
persistent identity: iGEM 2018 Plate3 Well14M
display id: iGEM_2018_Plate3_Well14M
version: 1
owned by: myers
member: BBa_K145300

iGEM 2018 Distribution Plate 3 Well 22E
type: Collection
persistent identity: iGEM 2018 Plate3 Well22E
display id: iGEM_2018_Plate3_Well22E
version: 1
owned by: myers
member: BBa_K137042

iGEM 2018 Distribution Plate 3 Well 14N
type: Collection
persistent identity: iGEM 2018 Plate3 Well14N
display id: iGEM_2018_Plate3_Well14N
version: 1
owned by: myers
member: BBa_I13405

iGEM 2018 Distribution Plate 3 Well 20A
type: Collection
persistent identity: iGEM 2018 Plate3 Well20A
display id: iGEM_2018_Plate3_Well20A
version: 1
owned by: myers
member: BBa_K118007

iGEM 2018 Distribution Plate 3 Well 21P
type: Collection
persistent identity: iGEM 2018 Plate3 Well21P
display id: iGEM_2018_Plate3_Well21P
version: 1
owned by: myers
member: BBa_K081016

iGEM 2018 Distribution Plate 3 Well 2N
type: Collection
persistent identity: iGEM 2018 Plate3 Well2N
display id: iGEM_2018_Plate3_Well2N
version: 1
owned by: myers
member: BBa_I6116

iGEM 2018 Distribution Plate 3 Well 17P
type: Collection
persistent identity: iGEM 2018 Plate3 Well17P
display id: iGEM_2018_Plate3_Well17P
version: 1
owned by: myers
member: BBa_I15008

iGEM 2018 Distribution Plate 3 Well 16F
type: Collection
persistent identity: iGEM 2018 Plate3 Well16F
display id: iGEM_2018_Plate3_Well16F
version: 1
owned by: myers
member: BBa_I13907

iGEM 2018 Distribution Plate 3 Well 22P
type: Collection
persistent identity: iGEM 2018 Plate3 Well22P
display id: iGEM_2018_Plate3_Well22P
version: 1
owned by: myers
member: BBa_I732913

iGEM 2018 Distribution Plate 3 Well 14K
type: Collection
persistent identity: iGEM 2018 Plate3 Well14K
display id: iGEM_2018_Plate3_Well14K
version: 1
owned by: myers
member: BBa_K145201

iGEM 2018 Distribution Plate 3 Well 22A
type: Collection
persistent identity: iGEM 2018 Plate3 Well22A
display id: iGEM_2018_Plate3_Well22A
version: 1
owned by: myers
member: BBa_K098991

iGEM 2018 Distribution Plate 3 Well 1E
type: Collection
persistent identity: iGEM 2018 Plate3 Well1E
display id: iGEM_2018_Plate3_Well1E
version: 1
owned by: myers
member: ECFP

iGEM 2018 Distribution Plate 3 Well 11N
type: Collection
persistent identity: iGEM 2018 Plate3 Well11N
display id: iGEM_2018_Plate3_Well11N
version: 1
owned by: myers
member: mRFP1

iGEM 2018 Distribution Plate 3 Well 12G
type: Collection
persistent identity: iGEM 2018 Plate3 Well12G
display id: iGEM_2018_Plate3_Well12G
version: 1
owned by: myers
member: RBS-GFP-Te

iGEM 2018 Distribution Plate 3 Well 23O
type: Collection
persistent identity: iGEM 2018 Plate3 Well23O
display id: iGEM_2018_Plate3_Well23O
version: 1
owned by: myers
member: BBa_J04450

iGEM 2018 Distribution Plate 3 Well 7O
type: Collection
persistent identity: iGEM 2018 Plate3 Well7O
display id: iGEM_2018_Plate3_Well7O
version: 1
owned by: myers
member: BBa_I732812

iGEM 2018 Distribution Plate 3 Well 15M
type: Collection
persistent identity: iGEM 2018 Plate3 Well15M
display id: iGEM_2018_Plate3_Well15M
version: 1
owned by: myers
member: BBa_I13452

iGEM 2018 Distribution Plate 3 Well 17D
type: Collection
persistent identity: iGEM 2018 Plate3 Well17D
display id: iGEM_2018_Plate3_Well17D
version: 1
owned by: myers
member: Prm +

BBa_J33201
type: ComponentDefinition
persistent identity: BBa J33201
display id: BBa_J33201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli chromosomal ars promoter with arsR repressor gene
creator: Chris French
created: 2006-10-12T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part consists of the promoter of the E. coli JM109 chromosomal arsenic detoxification operon (ars operon), including the ArsR repressor binding site and the arsR gene encoding the arsR repressor protein, together with its ribosome binding site. Addition of any other genes to the 3' end of this part will result in their expression being dependent on the presence of sodium arsenate or sodium arsenite. Arsenite or arsenite anion binds to the repressor protein ArsR, resulting in inability to repress the promoter. Based on our experiments, a concentration of 1 micromolar sodium arsenate in LB is sufficient for essentially full expression, though this will vary according to conditions.
mutable notes: Note that this sequence includes the arsR gene encoding the ArsR repressor protein, which is thus negatively autoregulated. No additional parts are required for arsenate-induced expression from this part. In principle, this part should also function in hosts other than E. coli.
mutable provenance: The DNA was derived by PCR using genomic DNA from E. coli JM109. Primers were designed based on the sequence reported by Diorio et al (1995), J. Bacteriol 177 (8), 2050-2056, Genbank accession X80057, GI:510824. The sequence given here is derived by sequencing the biobrick construct.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: -35 sequence
sequence annotation: -10 sequence
sequence annotation: rbs
sequence annotation: ArsR binding site
sequence annotation: arsR
sequence: https://synbiohub.org/public/igem/BBa_J33201_sequence/1

-35 sequence
type: SequenceAnnotation
persistent identity: annotation1902813
display id: annotation1902813
version: 1
top level: BBa_J33201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902813/range1902813/1

https://synbiohub.org/public/igem/BBa_J33201/annotation1902813/range1902813/1
type: Range
persistent identity: range1902813
display id: range1902813
version: 1
direction: direction/forward
top level: BBa_J33201
start: 57
end: 62
orientation: inline

-10 sequence
type: SequenceAnnotation
persistent identity: annotation1902814
display id: annotation1902814
version: 1
top level: BBa_J33201
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902814/range1902814/1

https://synbiohub.org/public/igem/BBa_J33201/annotation1902814/range1902814/1
type: Range
persistent identity: range1902814
display id: range1902814
version: 1
direction: direction/forward
top level: BBa_J33201
start: 80
end: 85
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1902815
display id: annotation1902815
version: 1
top level: BBa_J33201
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902815/range1902815/1

https://synbiohub.org/public/igem/BBa_J33201/annotation1902815/range1902815/1
type: Range
persistent identity: range1902815
display id: range1902815
version: 1
direction: direction/forward
top level: BBa_J33201
start: 108
end: 112
orientation: inline

ArsR binding site
type: SequenceAnnotation
persistent identity: annotation1902812
display id: annotation1902812
version: 1
top level: BBa_J33201
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902812/range1902812/1

https://synbiohub.org/public/igem/BBa_J33201/annotation1902812/range1902812/1
type: Range
persistent identity: range1902812
display id: range1902812
version: 1
direction: direction/forward
top level: BBa_J33201
start: 36
end: 54
orientation: inline

arsR
type: SequenceAnnotation
persistent identity: annotation1902816
display id: annotation1902816
version: 1
top level: BBa_J33201
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J33201/annotation1902816/range1902816/1

https://synbiohub.org/public/igem/BBa_J33201/annotation1902816/range1902816/1
type: Range
persistent identity: range1902816
display id: range1902816
version: 1
direction: direction/forward
top level: BBa_J33201
start: 119
end: 472
orientation: inline

BBa_K081020
type: ComponentDefinition
persistent identity: BBa K081020
display id: BBa_K081020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI and lasR protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a two-protein generator (cI and lasR) without transcriptional terminator. Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: cI: lasR:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_C0051
component: BBa_C0079
sequence annotation: BBa_C0079
sequence annotation: BBa_C0051
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081020_sequence/1

BBa_B0030
type: Component
persistent identity: component2249307
display id: component2249307
version: 1
top level: BBa_K081020
access: public
definition: BBa_B0030

BBa_C0051
type: Component
persistent identity: component2249312
display id: component2249312
version: 1
top level: BBa_K081020
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_C0079
type: Component
persistent identity: component2249320
display id: component2249320
version: 1
top level: BBa_K081020
access: public
definition: lasr

BBa_B0030
type: Component
persistent identity: component2249314
display id: component2249314
version: 1
top level: BBa_K081020
access: public
definition: BBa_B0030

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation2249320
display id: annotation2249320
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249320/range2249320/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K081020/annotation2249320/range2249320/1
type: Range
persistent identity: range2249320
display id: range2249320
version: 1
direction: direction/reverse
top level: BBa_K081020
start: 826
end: 1606
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2249312
display id: annotation2249312
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249312/range2249312/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K081020/annotation2249312/range2249312/1
type: Range
persistent identity: range2249312
display id: range2249312
version: 1
direction: direction/reverse
top level: BBa_K081020
start: 22
end: 796
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249307
display id: annotation2249307
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249307/range2249307/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081020/annotation2249307/range2249307/1
type: Range
persistent identity: range2249307
display id: range2249307
version: 1
direction: direction/forward
top level: BBa_K081020
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249314
display id: annotation2249314
version: 1
top level: BBa_K081020
location: https://synbiohub.org/public/igem/BBa_K081020/annotation2249314/range2249314/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081020/annotation2249314/range2249314/1
type: Range
persistent identity: range2249314
display id: range2249314
version: 1
direction: direction/forward
top level: BBa_K081020
start: 805
end: 819
orientation: inline

BBa_J09855
type: ComponentDefinition
persistent identity: BBa J09855
display id: BBa_J09855
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive luxR device with pLuxR
creator: Jon Badalamenti
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _72_40_
m user id: 0
owner id: 475
owning group id: 72
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: I14032.I0462.R0062
mutable notes: This is a universal part designed to allow induction by HSL of whatever is assembled downstream of the part.
mutable provenance: This part was constructed from registry biobricks.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I14032
component: BBa_I0462
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_I14032
sequence annotation: BBa_I0462
sequence: https://synbiohub.org/public/igem/BBa_J09855_sequence/1

BBa_I14032
type: Component
persistent identity: component2260430
display id: component2260430
version: 1
top level: BBa_J09855
access: public
definition: P(Lac) IQ

BBa_I0462
type: Component
persistent identity: component2260445
display id: component2260445
version: 1
top level: BBa_J09855
access: public
definition: https://synbiohub.org/public/igem/BBa_I0462/1

BBa_R0062
type: Component
persistent identity: component2260447
display id: component2260447
version: 1
top level: BBa_J09855
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2260447
display id: annotation2260447
version: 1
top level: BBa_J09855
location: https://synbiohub.org/public/igem/BBa_J09855/annotation2260447/range2260447/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_J09855/annotation2260447/range2260447/1
type: Range
persistent identity: range2260447
display id: range2260447
version: 1
direction: direction/forward
top level: BBa_J09855
start: 990
end: 1044
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2260430
display id: annotation2260430
version: 1
top level: BBa_J09855
location: https://synbiohub.org/public/igem/BBa_J09855/annotation2260430/range2260430/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_J09855/annotation2260430/range2260430/1
type: Range
persistent identity: range2260430
display id: range2260430
version: 1
direction: direction/reverse
top level: BBa_J09855
start: 1
end: 37
orientation: inline

BBa_I0462
type: SequenceAnnotation
persistent identity: annotation2260445
display id: annotation2260445
version: 1
top level: BBa_J09855
location: https://synbiohub.org/public/igem/BBa_J09855/annotation2260445/range2260445/1
component: BBa_I0462

https://synbiohub.org/public/igem/BBa_J09855/annotation2260445/range2260445/1
type: Range
persistent identity: range2260445
display id: range2260445
version: 1
direction: direction/reverse
top level: BBa_J09855
start: 46
end: 981
orientation: inline

BBa_I15015
type: ComponentDefinition
persistent identity: BBa I15015
display id: BBa_I15015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: polycistronic ECFP+EYFP under B0032
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to produce a single mRNA encoding ECFP and EYFP under the medium RBS B0032
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0032
component: BBa_E0020
component: BBa_E0030
sequence annotation: BBa_B0032
sequence annotation: BBa_E0020
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I15015_sequence/1

BBa_B0032
type: Component
persistent identity: component1478379
display id: component1478379
version: 1
top level: BBa_I15015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0032
type: Component
persistent identity: component1478390
display id: component1478390
version: 1
top level: BBa_I15015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E0020
type: Component
persistent identity: component1478382
display id: component1478382
version: 1
top level: BBa_I15015
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_E0030
type: Component
persistent identity: component1478393
display id: component1478393
version: 1
top level: BBa_I15015
access: public
definition: eyfp

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478390
display id: annotation1478390
version: 1
top level: BBa_I15015
location: https://synbiohub.org/public/igem/BBa_I15015/annotation1478390/range1478390/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15015/annotation1478390/range1478390/1
type: Range
persistent identity: range1478390
display id: range1478390
version: 1
direction: direction/forward
top level: BBa_I15015
start: 751
end: 763
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1478382
display id: annotation1478382
version: 1
top level: BBa_I15015
location: https://synbiohub.org/public/igem/BBa_I15015/annotation1478382/range1478382/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I15015/annotation1478382/range1478382/1
type: Range
persistent identity: range1478382
display id: range1478382
version: 1
direction: direction/forward
top level: BBa_I15015
start: 20
end: 742
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478379
display id: annotation1478379
version: 1
top level: BBa_I15015
location: https://synbiohub.org/public/igem/BBa_I15015/annotation1478379/range1478379/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15015/annotation1478379/range1478379/1
type: Range
persistent identity: range1478379
display id: range1478379
version: 1
direction: direction/forward
top level: BBa_I15015
start: 1
end: 13
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1478393
display id: annotation1478393
version: 1
top level: BBa_I15015
location: https://synbiohub.org/public/igem/BBa_I15015/annotation1478393/range1478393/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I15015/annotation1478393/range1478393/1
type: Range
persistent identity: range1478393
display id: range1478393
version: 1
direction: direction/forward
top level: BBa_I15015
start: 770
end: 1492
orientation: inline

BBa_K105004
type: ComponentDefinition
persistent identity: BBa K105004
display id: BBa_K105004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI - repressor from E.coli phage lambda
creator: Katja Karstens
created: 2008-10-11T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: cI is a repressor of the phage lamda. It binds specificly to a operator site ( BBa_R0051 & BBa_K105021).
mutable notes: To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006).
mutable provenance: We changed the format of the cI coding part of BBa_C0051 into Phillips/Silver standard.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K105004_sequence/1

BBa_I0407
type: ComponentDefinition
persistent identity: BBa I0407
display id: BBa_I0407
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0034.C0078.E0420
creator: jcbraff
created: 2004-06-08T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0040.B0034.C0078.E0420
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0034
component: BBa_C0078
component: BBa_R0040
component: BBa_E0020
component: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0078
sequence annotation: BBa_E0020
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0407_sequence/1

BBa_B0010
type: Component
persistent identity: component958754
display id: component958754
version: 1
top level: BBa_I0407
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component958747
display id: component958747
version: 1
top level: BBa_I0407
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0078
type: Component
persistent identity: component958741
display id: component958741
version: 1
top level: BBa_I0407
access: public
definition: lasI

BBa_R0040
type: Component
persistent identity: component958723
display id: component958723
version: 1
top level: BBa_I0407
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0020
type: Component
persistent identity: component958749
display id: component958749
version: 1
top level: BBa_I0407
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0012
type: Component
persistent identity: component958764
display id: component958764
version: 1
top level: BBa_I0407
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component958731
display id: component958731
version: 1
top level: BBa_I0407
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation958723
display id: annotation958723
version: 1
top level: BBa_I0407
location: https://synbiohub.org/public/igem/BBa_I0407/annotation958723/range958723/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I0407/annotation958723/range958723/1
type: Range
persistent identity: range958723
display id: range958723
version: 1
direction: direction/forward
top level: BBa_I0407
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation958747
display id: annotation958747
version: 1
top level: BBa_I0407
location: https://synbiohub.org/public/igem/BBa_I0407/annotation958747/range958747/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0407/annotation958747/range958747/1
type: Range
persistent identity: range958747
display id: range958747
version: 1
direction: direction/forward
top level: BBa_I0407
start: 756
end: 767
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation958754
display id: annotation958754
version: 1
top level: BBa_I0407
location: https://synbiohub.org/public/igem/BBa_I0407/annotation958754/range958754/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0407/annotation958754/range958754/1
type: Range
persistent identity: range958754
display id: range958754
version: 1
direction: direction/forward
top level: BBa_I0407
start: 1505
end: 1584
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation958741
display id: annotation958741
version: 1
top level: BBa_I0407
location: https://synbiohub.org/public/igem/BBa_I0407/annotation958741/range958741/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_I0407/annotation958741/range958741/1
type: Range
persistent identity: range958741
display id: range958741
version: 1
direction: direction/forward
top level: BBa_I0407
start: 81
end: 722
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation958749
display id: annotation958749
version: 1
top level: BBa_I0407
location: https://synbiohub.org/public/igem/BBa_I0407/annotation958749/range958749/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I0407/annotation958749/range958749/1
type: Range
persistent identity: range958749
display id: range958749
version: 1
direction: direction/forward
top level: BBa_I0407
start: 774
end: 1496
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation958764
display id: annotation958764
version: 1
top level: BBa_I0407
location: https://synbiohub.org/public/igem/BBa_I0407/annotation958764/range958764/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0407/annotation958764/range958764/1
type: Range
persistent identity: range958764
display id: range958764
version: 1
direction: direction/forward
top level: BBa_I0407
start: 1593
end: 1633
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation958731
display id: annotation958731
version: 1
top level: BBa_I0407
location: https://synbiohub.org/public/igem/BBa_I0407/annotation958731/range958731/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0407/annotation958731/range958731/1
type: Range
persistent identity: range958731
display id: range958731
version: 1
direction: direction/forward
top level: BBa_I0407
start: 63
end: 74
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0078
display id: BBa_C0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa
creator: Chris Walsh (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for lasI protein, which produces the chemical signal AI-1
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LasI
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0078_sequence/1

LasI
type: SequenceAnnotation
persistent identity: annotation305970
display id: annotation305970
version: 1
top level: lasI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1

https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
type: Range
persistent identity: range305970
display id: range305970
version: 1
direction: direction/forward
top level: lasI
start: 1
end: 603
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214003
display id: annotation2214003
version: 1
top level: lasI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1

https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
type: Range
persistent identity: range2214003
display id: range2214003
version: 1
direction: direction/forward
top level: lasI
start: 643
end: 667
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306608
display id: annotation306608
version: 1
top level: lasI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1

https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
type: Range
persistent identity: range306608
display id: range306608
version: 1
direction: direction/forward
top level: lasI
start: 604
end: 636
orientation: inline

Mnt
type: ComponentDefinition
persistent identity: BBa R0073
display id: BBa_R0073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (Mnt regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable notes: unsure of where -35 site is
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: mnt
sequence annotation: dimer left half
sequence annotation: -10
sequence annotation: dimer right half
sequence: https://synbiohub.org/public/igem/BBa_R0073_sequence/1

mnt
type: SequenceAnnotation
persistent identity: annotation302669
display id: annotation302669
version: 1
top level: Mnt
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0073/annotation302669/range302669/1

https://synbiohub.org/public/igem/BBa_R0073/annotation302669/range302669/1
type: Range
persistent identity: range302669
display id: range302669
version: 1
direction: direction/forward
top level: Mnt
start: 1
end: 67
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation318636
display id: annotation318636
version: 1
top level: Mnt
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318636/range318636/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318636/range318636/1
type: Range
persistent identity: range318636
display id: range318636
version: 1
direction: direction/forward
top level: Mnt
start: 23
end: 29
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318643
display id: annotation318643
version: 1
top level: Mnt
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318643/range318643/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318643/range318643/1
type: Range
persistent identity: range318643
display id: range318643
version: 1
direction: direction/forward
top level: Mnt
start: 47
end: 52
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation318638
display id: annotation318638
version: 1
top level: Mnt
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0073/annotation318638/range318638/1

https://synbiohub.org/public/igem/BBa_R0073/annotation318638/range318638/1
type: Range
persistent identity: range318638
display id: range318638
version: 1
direction: direction/forward
top level: Mnt
start: 32
end: 38
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R1051
display id: BBa_R1051
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (lambda cI regulated)
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Modified to comply with BBa_S0001: TTGACA-17N-GATACT Incompatible with host expressing cI repressor.
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: -35
sequence annotation: OR2
sequence annotation: BBa_R1051
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1051_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2076
display id: annotation2076
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
type: Range
persistent identity: range2076
display id: range2076
version: 1
direction: direction/forward
top level: cI lam
start: 25
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2075
display id: annotation2075
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
type: Range
persistent identity: range2075
display id: range2075
version: 1
direction: direction/forward
top level: cI lam
start: 15
end: 20
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2077
display id: annotation2077
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
type: Range
persistent identity: range2077
display id: range2077
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 17
orientation: inline

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation7074
display id: annotation7074
version: 1
top level: cI lam
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1

https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
type: Range
persistent identity: range7074
display id: range7074
version: 1
direction: direction/forward
top level: cI lam
start: 1
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2078
display id: annotation2078
version: 1
top level: cI lam
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1

https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
type: Range
persistent identity: range2078
display id: range2078
version: 1
direction: direction/forward
top level: cI lam
start: 38
end: 43
orientation: inline

BBa_J04450
type: ComponentDefinition
persistent identity: BBa J04450
display id: BBa_J04450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP Coding Device
creator: Tamar Odle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 328
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains an IPTG inducible promoter an RBS, RFP, and terminator.
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_R0010
component: BBa_B0012
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_J04450_sequence/1

BBa_B0010
type: Component
persistent identity: component1509417
display id: component1509417
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0010
type: Component
persistent identity: component1509394
display id: component1509394
version: 1
top level: BBa_J04450
access: public
definition: LacI

BBa_B0012
type: Component
persistent identity: component1509427
display id: component1509427
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1509404
display id: component1509404
version: 1
top level: BBa_J04450
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1509411
display id: component1509411
version: 1
top level: BBa_J04450
access: public
definition: mRFP1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1509404
display id: annotation1509404
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04450/annotation1509404/range1509404/1
type: Range
persistent identity: range1509404
display id: range1509404
version: 1
direction: direction/forward
top level: BBa_J04450
start: 209
end: 220
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1509417
display id: annotation1509417
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509417/range1509417/1
type: Range
persistent identity: range1509417
display id: range1509417
version: 1
direction: direction/forward
top level: BBa_J04450
start: 941
end: 1020
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1509411
display id: annotation1509411
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509411/range1509411/1
type: Range
persistent identity: range1509411
display id: range1509411
version: 1
direction: direction/forward
top level: BBa_J04450
start: 227
end: 907
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1509427
display id: annotation1509427
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04450/annotation1509427/range1509427/1
type: Range
persistent identity: range1509427
display id: range1509427
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1029
end: 1069
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1509394
display id: annotation1509394
version: 1
top level: BBa_J04450
location: https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04450/annotation1509394/range1509394/1
type: Range
persistent identity: range1509394
display id: range1509394
version: 1
direction: direction/forward
top level: BBa_J04450
start: 1
end: 200
orientation: inline

BBa_S03591
type: ComponentDefinition
persistent identity: BBa S03591
display id: BBa_S03591
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AraCPCrev : TT
creator: Davidson and Missouri Western
created: 2006-09-04T11:00:00Z
modified: 2015-05-08T01:14:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0010
component: BBa_I13458
sequence annotation: BBa_B0012
sequence annotation: BBa_I13458
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S03591_sequence/1

BBa_B0012
type: Component
persistent identity: component1900573
display id: component1900573
version: 1
top level: BBa_S03591
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1900571
display id: component1900571
version: 1
top level: BBa_S03591
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13458
type: Component
persistent identity: component1900570
display id: component1900570
version: 1
top level: BBa_S03591
access: public
definition: BBa_I13458

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1900573
display id: annotation1900573
version: 1
top level: BBa_S03591
location: https://synbiohub.org/public/igem/BBa_S03591/annotation1900573/range1900573/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03591/annotation1900573/range1900573/1
type: Range
persistent identity: range1900573
display id: range1900573
version: 1
direction: direction/forward
top level: BBa_S03591
start: 1211
end: 1251
orientation: inline

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation1900570
display id: annotation1900570
version: 1
top level: BBa_S03591
location: https://synbiohub.org/public/igem/BBa_S03591/annotation1900570/range1900570/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_S03591/annotation1900570/range1900570/1
type: Range
persistent identity: range1900570
display id: range1900570
version: 1
direction: direction/reverse
top level: BBa_S03591
start: 1
end: 1114
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1900571
display id: annotation1900571
version: 1
top level: BBa_S03591
location: https://synbiohub.org/public/igem/BBa_S03591/annotation1900571/range1900571/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03591/annotation1900571/range1900571/1
type: Range
persistent identity: range1900571
display id: range1900571
version: 1
direction: direction/forward
top level: BBa_S03591
start: 1123
end: 1202
orientation: inline

BBa_I13973
type: ComponentDefinition
persistent identity: BBa I13973
display id: BBa_I13973
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: c1 promoter + YFP-aav reporter device
creator: jasonk
created: 2004-09-22T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 2
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is meant to serve as a reporter device for the synchronized oscillator.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_E0034
component: BBa_B0010
component: BBa_B0034
component: BBa_R0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0051
sequence annotation: BBa_B0010
sequence annotation: BBa_E0034
sequence: https://synbiohub.org/public/igem/BBa_I13973_sequence/1

BBa_B0012
type: Component
persistent identity: component1243955
display id: component1243955
version: 1
top level: BBa_I13973
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0034
type: Component
persistent identity: component1243940
display id: component1243940
version: 1
top level: BBa_I13973
access: public
definition: EYFP

BBa_B0010
type: Component
persistent identity: component1243945
display id: component1243945
version: 1
top level: BBa_I13973
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1243938
display id: component1243938
version: 1
top level: BBa_I13973
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0051
type: Component
persistent identity: component1243930
display id: component1243930
version: 1
top level: BBa_I13973
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1243955
display id: annotation1243955
version: 1
top level: BBa_I13973
location: https://synbiohub.org/public/igem/BBa_I13973/annotation1243955/range1243955/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13973/annotation1243955/range1243955/1
type: Range
persistent identity: range1243955
display id: range1243955
version: 1
direction: direction/forward
top level: BBa_I13973
start: 934
end: 974
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1243938
display id: annotation1243938
version: 1
top level: BBa_I13973
location: https://synbiohub.org/public/igem/BBa_I13973/annotation1243938/range1243938/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13973/annotation1243938/range1243938/1
type: Range
persistent identity: range1243938
display id: range1243938
version: 1
direction: direction/forward
top level: BBa_I13973
start: 58
end: 69
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1243930
display id: annotation1243930
version: 1
top level: BBa_I13973
location: https://synbiohub.org/public/igem/BBa_I13973/annotation1243930/range1243930/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I13973/annotation1243930/range1243930/1
type: Range
persistent identity: range1243930
display id: range1243930
version: 1
direction: direction/forward
top level: BBa_I13973
start: 1
end: 49
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1243945
display id: annotation1243945
version: 1
top level: BBa_I13973
location: https://synbiohub.org/public/igem/BBa_I13973/annotation1243945/range1243945/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13973/annotation1243945/range1243945/1
type: Range
persistent identity: range1243945
display id: range1243945
version: 1
direction: direction/forward
top level: BBa_I13973
start: 846
end: 925
orientation: inline

BBa_E0034
type: SequenceAnnotation
persistent identity: annotation1243940
display id: annotation1243940
version: 1
top level: BBa_I13973
location: https://synbiohub.org/public/igem/BBa_I13973/annotation1243940/range1243940/1
component: BBa_E0034

https://synbiohub.org/public/igem/BBa_I13973/annotation1243940/range1243940/1
type: Range
persistent identity: range1243940
display id: range1243940
version: 1
direction: direction/forward
top level: BBa_I13973
start: 76
end: 837
orientation: inline

BBa_J22101
type: ComponentDefinition
persistent identity: BBa J22101
display id: BBa_J22101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac Y gene
creator: Krithiga Sankaran
created: 2006-09-03T11:00:00Z
modified: 2015-08-31T04:08:38Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _70_
m user id: 0
owner id: 1038
owning group id: 70
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The Lactose permase (Lac Y) gene.
mutable notes: NA
mutable provenance: The Lac Y gene has been PCR amplified from the chromosomal DNA of MG1655 (Escherechia coli)and has been inserted into plasmid pSB1A2( Part number BBa_B0015).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J22101_sequence/1

luxR
type: ComponentDefinition
persistent identity: BBa R0061
display id: BBa_R0061
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (HSL-mediated luxR repressor)
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
created: 2004-01-28T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Lux Box
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0061_sequence/1

Lux Box
type: SequenceAnnotation
persistent identity: annotation305877
display id: annotation305877
version: 1
top level: luxR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1
type: Range
persistent identity: range305877
display id: range305877
version: 1
direction: direction/forward
top level: luxR
start: 6
end: 25
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation305863
display id: annotation305863
version: 1
top level: luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1
type: Range
persistent identity: range305863
display id: range305863
version: 1
direction: direction/forward
top level: luxR
start: 1
end: 6
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation305864
display id: annotation305864
version: 1
top level: luxR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1

https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1
type: Range
persistent identity: range305864
display id: range305864
version: 1
direction: direction/forward
top level: luxR
start: 25
end: 30
orientation: inline

BBa_I714044
type: ComponentDefinition
persistent identity: BBa I714044
display id: BBa_I714044
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [R0010][I714031]
creator: Mingzhi Qu
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is I714031 with R0010 Promoter in the front.
mutable provenance: [R0010]+[I714031]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0010
component: BBa_I714031
sequence annotation: BBa_R0010
sequence annotation: BBa_I714031
sequence: https://synbiohub.org/public/igem/BBa_I714044_sequence/1

BBa_R0010
type: Component
persistent identity: component1951654
display id: component1951654
version: 1
top level: BBa_I714044
access: public
definition: LacI

BBa_I714031
type: Component
persistent identity: component1951661
display id: component1951661
version: 1
top level: BBa_I714044
access: public
definition: BBa_I714031

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1951654
display id: annotation1951654
version: 1
top level: BBa_I714044
location: https://synbiohub.org/public/igem/BBa_I714044/annotation1951654/range1951654/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I714044/annotation1951654/range1951654/1
type: Range
persistent identity: range1951654
display id: range1951654
version: 1
direction: direction/forward
top level: BBa_I714044
start: 1
end: 200
orientation: inline

BBa_I714031
type: SequenceAnnotation
persistent identity: annotation1951661
display id: annotation1951661
version: 1
top level: BBa_I714044
location: https://synbiohub.org/public/igem/BBa_I714044/annotation1951661/range1951661/1
component: BBa_I714031

https://synbiohub.org/public/igem/BBa_I714044/annotation1951661/range1951661/1
type: Range
persistent identity: range1951661
display id: range1951661
version: 1
direction: direction/reverse
top level: BBa_I714044
start: 209
end: 486
orientation: inline

lacZ-alpha
type: ComponentDefinition
persistent identity: BBa I732006
display id: BBa_I732006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ alpha fragment
creator: Zhan Jian
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
mutable notes: None
mutable provenance: PCR amplified from BBa_J33202 without the RBS.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: STOP
sequence annotation: lacZ
sequence annotation: START
sequence: https://synbiohub.org/public/igem/BBa_I732006_sequence/1

STOP
type: SequenceAnnotation
persistent identity: annotation1936906
display id: annotation1936906
version: 1
top level: lacZ-alpha
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1

https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
type: Range
persistent identity: range1936906
display id: range1936906
version: 1
direction: direction/forward
top level: lacZ-alpha
start: 229
end: 234
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936904
display id: annotation1936904
version: 1
top level: lacZ-alpha
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1

https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
type: Range
persistent identity: range1936904
display id: range1936904
version: 1
direction: direction/forward
top level: lacZ-alpha
start: 1
end: 234
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation1936905
display id: annotation1936905
version: 1
top level: lacZ-alpha
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1

https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
type: Range
persistent identity: range1936905
display id: range1936905
version: 1
direction: direction/forward
top level: lacZ-alpha
start: 1
end: 3
orientation: inline

BBa_I746361
type: ComponentDefinition
persistent identity: BBa I746361
display id: BBa_I746361
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PO promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter.
mutable notes: no special considerations
mutable provenance: The source of the DNA is the P2 phage genome.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PO
sequence: https://synbiohub.org/public/igem/BBa_I746361_sequence/1

PO
type: SequenceAnnotation
persistent identity: annotation1943784
display id: annotation1943784
version: 1
top level: BBa_I746361
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1

https://synbiohub.org/public/igem/BBa_I746361/annotation1943784/range1943784/1
type: Range
persistent identity: range1943784
display id: range1943784
version: 1
direction: direction/forward
top level: BBa_I746361
start: 1
end: 92
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12006
display id: BBa_I12006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (repressed by 434 cI)
creator: mcnamara
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
mutable notes: The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
mutable provenance: Bushman(1993), Shih & Gussin (1983)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 lambda
sequence annotation: OR1 434
sequence annotation: OR2 lambda
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I12006_sequence/1

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation786500
display id: annotation786500
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
type: Range
persistent identity: range786500
display id: range786500
version: 1
direction: direction/forward
top level: Prm +
start: 9
end: 25
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation786365
display id: annotation786365
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
type: Range
persistent identity: range786365
display id: range786365
version: 1
direction: direction/forward
top level: Prm +
start: 56
end: 69
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation786518
display id: annotation786518
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1

https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
type: Range
persistent identity: range786518
display id: range786518
version: 1
direction: direction/forward
top level: Prm +
start: 33
end: 49
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation837228
display id: annotation837228
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
type: Range
persistent identity: range837228
display id: range837228
version: 1
direction: direction/forward
top level: Prm +
start: 71
end: 76
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation837284
display id: annotation837284
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1

https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
type: Range
persistent identity: range837284
display id: range837284
version: 1
direction: direction/forward
top level: Prm +
start: 48
end: 53
orientation: inline

BBa_I13602
type: ComponentDefinition
persistent identity: BBa I13602
display id: BBa_I13602
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet operator with CFP reporter (with LVA tag) [R/Tc+]
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
component: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_E0022
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I13602_sequence/1

BBa_B0034
type: Component
persistent identity: component947262
display id: component947262
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component947292
display id: component947292
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component947282
display id: component947282
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component947254
display id: component947254
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0022
type: Component
persistent identity: component947274
display id: component947274
version: 1
top level: BBa_I13602
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947282
display id: annotation947282
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947282/range947282/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13602/annotation947282/range947282/1
type: Range
persistent identity: range947282
display id: range947282
version: 1
direction: direction/forward
top level: BBa_I13602
start: 851
end: 930
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947262
display id: annotation947262
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947262/range947262/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13602/annotation947262/range947262/1
type: Range
persistent identity: range947262
display id: range947262
version: 1
direction: direction/forward
top level: BBa_I13602
start: 63
end: 74
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947292
display id: annotation947292
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947292/range947292/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13602/annotation947292/range947292/1
type: Range
persistent identity: range947292
display id: range947292
version: 1
direction: direction/forward
top level: BBa_I13602
start: 939
end: 979
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation947274
display id: annotation947274
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947274/range947274/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I13602/annotation947274/range947274/1
type: Range
persistent identity: range947274
display id: range947274
version: 1
direction: direction/forward
top level: BBa_I13602
start: 81
end: 842
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947254
display id: annotation947254
version: 1
top level: BBa_I13602
location: https://synbiohub.org/public/igem/BBa_I13602/annotation947254/range947254/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13602/annotation947254/range947254/1
type: Range
persistent identity: range947254
display id: range947254
version: 1
direction: direction/forward
top level: BBa_I13602
start: 1
end: 54
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0077
display id: BBa_R0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated, RBS+)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CinR (BBa_C0077) in complex with O3-C14:1-HSL (from CinI, BBa_C0076) binds to this promoter and activates transcription. This promoter contains the RBS: TGGAGG
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: the last 5 bases (gctaa) removed to allow for the correct spacing between the RBS (TGGAGG) and the start of the downstream gene after biobricks splicing.
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: embedded RBS
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_R0077_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation301371
display id: annotation301371
version: 1
top level: cinR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301371/range301371/1
type: Range
persistent identity: range301371
display id: range301371
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

embedded RBS
type: SequenceAnnotation
persistent identity: annotation301150
display id: annotation301150
version: 1
top level: cinR
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301150/range301150/1
type: Range
persistent identity: range301150
display id: range301150
version: 1
direction: direction/forward
top level: cinR
start: 226
end: 231
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301205
display id: annotation301205
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301205/range301205/1
type: Range
persistent identity: range301205
display id: range301205
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation301204
display id: annotation301204
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1

https://synbiohub.org/public/igem/BBa_R0077/annotation301204/range301204/1
type: Range
persistent identity: range301204
display id: range301204
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

BBa_I13504
type: ComponentDefinition
persistent identity: BBa I13504
display id: BBa_I13504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0040
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13504_sequence/1

BBa_E0040
type: Component
persistent identity: component1505069
display id: component1505069
version: 1
top level: BBa_I13504
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1505074
display id: component1505074
version: 1
top level: BBa_I13504
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1505067
display id: component1505067
version: 1
top level: BBa_I13504
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1505084
display id: component1505084
version: 1
top level: BBa_I13504
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505067
display id: annotation1505067
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13504/annotation1505067/range1505067/1
type: Range
persistent identity: range1505067
display id: range1505067
version: 1
direction: direction/forward
top level: BBa_I13504
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505069
display id: annotation1505069
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13504/annotation1505069/range1505069/1
type: Range
persistent identity: range1505069
display id: range1505069
version: 1
direction: direction/forward
top level: BBa_I13504
start: 19
end: 738
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505084
display id: annotation1505084
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13504/annotation1505084/range1505084/1
type: Range
persistent identity: range1505084
display id: range1505084
version: 1
direction: direction/forward
top level: BBa_I13504
start: 835
end: 875
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505074
display id: annotation1505074
version: 1
top level: BBa_I13504
location: https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13504/annotation1505074/range1505074/1
type: Range
persistent identity: range1505074
display id: range1505074
version: 1
direction: direction/forward
top level: BBa_I13504
start: 747
end: 826
orientation: inline

ARL2A0101
type: ComponentDefinition
persistent identity: BBa I732105
display id: BBa_I732105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0101
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732105_sequence/1

rhIR
type: ComponentDefinition
persistent identity: BBa C0171
display id: BBa_C0171
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlR repressor/activator from P. aeruginosa PA3477 (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0071 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0171_sequence/1

BBa_K118002
type: ComponentDefinition
persistent identity: BBa K118002
display id: BBa_K118002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtB coding sequence encoding phytoene synthase
creator: Andrew Hall
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
mutable notes: No special considerations.
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: crtB coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118002_sequence/1

crtB coding sequence
type: SequenceAnnotation
persistent identity: annotation1995701
display id: annotation1995701
version: 1
top level: BBa_K118002
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1

https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
type: Range
persistent identity: range1995701
display id: range1995701
version: 1
direction: direction/forward
top level: BBa_K118002
start: 1
end: 930
orientation: inline

ARL2A0203
type: ComponentDefinition
persistent identity: BBa I732110
display id: BBa_I732110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0203
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732110_sequence/1

BBa_M0100
type: ComponentDefinition
persistent identity: BBa M0100
display id: BBa_M0100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: tRNA_Arg5 to use as transcriptional reporter
creator: jcbraff
created: 2005-03-25T12:00:00Z
modified: 2015-05-08T01:13:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: tRNA_Arg5 to use as transcriptional reporter. Sequence is natural tRNA_Arg5 except with A to U mutation near its 3' end.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_M0100_sequence/1

BBa_K137055
type: ComponentDefinition
persistent identity: BBa K137055
display id: BBa_K137055
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive pabA expression construct
creator: Victoria Hsiao
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 2987
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: PabA is one of three genes (pabA, pabB, pabC) involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter j23100 and the strong RBS b0034, and in front of the terminator b0015. Currently it is in the inducible copy plasmid psb2k3, which can be induced to high copy via IPTG.
mutable notes: The purpose of this construct is to test the effects of pabA overexpression on the total production of pABA from chorismate. pABA is a necessary structural component in the synthesis of folate and so the hope is that by increasing the flux through the pABA synthesis pathway, we will be able to increase the total production of folate.
mutable provenance: pabA has been extracted from the genome of L.lactis subspe. IL1403.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0010
component: BBa_B0034
component: BBa_J23100
component: BBa_K137005
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_J23100
sequence annotation: BBa_K137005
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K137055_sequence/1

BBa_B0010
type: Component
persistent identity: component1969115
display id: component1969115
version: 1
top level: BBa_K137055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1969113
display id: component1969113
version: 1
top level: BBa_K137055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J23100
type: Component
persistent identity: component1969111
display id: component1969111
version: 1
top level: BBa_K137055
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_K137005
type: Component
persistent identity: component1969114
display id: component1969114
version: 1
top level: BBa_K137055
access: public
definition: https://synbiohub.org/public/igem/BBa_K137005/1

BBa_B0012
type: Component
persistent identity: component1969117
display id: component1969117
version: 1
top level: BBa_K137055
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1969117
display id: annotation1969117
version: 1
top level: BBa_K137055
location: https://synbiohub.org/public/igem/BBa_K137055/annotation1969117/range1969117/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137055/annotation1969117/range1969117/1
type: Range
persistent identity: range1969117
display id: range1969117
version: 1
direction: direction/forward
top level: BBa_K137055
start: 743
end: 783
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1969111
display id: annotation1969111
version: 1
top level: BBa_K137055
location: https://synbiohub.org/public/igem/BBa_K137055/annotation1969111/range1969111/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K137055/annotation1969111/range1969111/1
type: Range
persistent identity: range1969111
display id: range1969111
version: 1
direction: direction/reverse
top level: BBa_K137055
start: 1
end: 35
orientation: inline

BBa_K137005
type: SequenceAnnotation
persistent identity: annotation1969114
display id: annotation1969114
version: 1
top level: BBa_K137055
location: https://synbiohub.org/public/igem/BBa_K137055/annotation1969114/range1969114/1
component: BBa_K137005

https://synbiohub.org/public/igem/BBa_K137055/annotation1969114/range1969114/1
type: Range
persistent identity: range1969114
display id: range1969114
version: 1
direction: direction/reverse
top level: BBa_K137055
start: 62
end: 646
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1969115
display id: annotation1969115
version: 1
top level: BBa_K137055
location: https://synbiohub.org/public/igem/BBa_K137055/annotation1969115/range1969115/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137055/annotation1969115/range1969115/1
type: Range
persistent identity: range1969115
display id: range1969115
version: 1
direction: direction/forward
top level: BBa_K137055
start: 655
end: 734
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1969113
display id: annotation1969113
version: 1
top level: BBa_K137055
location: https://synbiohub.org/public/igem/BBa_K137055/annotation1969113/range1969113/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137055/annotation1969113/range1969113/1
type: Range
persistent identity: range1969113
display id: range1969113
version: 1
direction: direction/reverse
top level: BBa_K137055
start: 44
end: 55
orientation: inline

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Second of two required phycocyanobilin biosynthetic genes.
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PcyA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
direction: direction/forward
top level: BBa_I15009
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
direction: direction/forward
top level: BBa_I15009
start: 751
end: 775
orientation: inline

BBa_J52021
type: ComponentDefinition
persistent identity: BBa J52021
display id: BBa_J52021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnTraf6-linker-GFP
creator: Matej Skocaj
created: 2006-10-13T11:00:00Z
modified: 2016-01-26T09:38:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _80_
m user id: 0
m user id: 4206
owner id: 857
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Part is composed of TIR domain of Traf6 and GFP linked with 6 aminoacids long linker. GFP serves as reporter. For usage in eukaryotes.
mutable notes: None.
mutable provenance: dnTraf6- pDeNy GFP-
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence annotation: dnTraf6-linker-GFP
sequence annotation: linker
sequence annotation: dnTraf6
sequence: https://synbiohub.org/public/igem/BBa_J52021_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation1903261
display id: annotation1903261
version: 1
top level: BBa_J52021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903261/range1903261/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903261/range1903261/1
type: Range
persistent identity: range1903261
display id: range1903261
version: 1
direction: direction/forward
top level: BBa_J52021
start: 733
end: 1446
orientation: inline

dnTraf6-linker-GFP
type: SequenceAnnotation
persistent identity: annotation1903262
display id: annotation1903262
version: 1
top level: BBa_J52021
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903262/range1903262/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903262/range1903262/1
type: Range
persistent identity: range1903262
display id: range1903262
version: 1
direction: direction/forward
top level: BBa_J52021
start: 1
end: 1446
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903260
display id: annotation1903260
version: 1
top level: BBa_J52021
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903260/range1903260/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903260/range1903260/1
type: Range
persistent identity: range1903260
display id: range1903260
version: 1
direction: direction/forward
top level: BBa_J52021
start: 715
end: 732
orientation: inline

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903259
display id: annotation1903259
version: 1
top level: BBa_J52021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52021/annotation1903259/range1903259/1

https://synbiohub.org/public/igem/BBa_J52021/annotation1903259/range1903259/1
type: Range
persistent identity: range1903259
display id: range1903259
version: 1
direction: direction/forward
top level: BBa_J52021
start: 1
end: 714
orientation: inline

BBa_I718013
type: ComponentDefinition
persistent identity: BBa I718013
display id: BBa_I718013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ter-lox66-rbs-dapA Coli
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: downstream construct of the synthetic multicellular bacterium
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0030
component: BBa_I718016
component: BBa_B0012
component: BBa_B0010
component: BBa_I718004
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I718016
sequence annotation: BBa_B0030
sequence annotation: BBa_I718004
sequence: https://synbiohub.org/public/igem/BBa_I718013_sequence/1

BBa_B0030
type: Component
persistent identity: component1956316
display id: component1956316
version: 1
top level: BBa_I718013
access: public
definition: BBa_B0030

BBa_I718016
type: Component
persistent identity: component1956314
display id: component1956314
version: 1
top level: BBa_I718013
access: public
definition: https://synbiohub.org/public/igem/BBa_I718016/1

BBa_B0012
type: Component
persistent identity: component1956310
display id: component1956310
version: 1
top level: BBa_I718013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1956308
display id: component1956308
version: 1
top level: BBa_I718013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I718004
type: Component
persistent identity: component1956321
display id: component1956321
version: 1
top level: BBa_I718013
access: public
definition: https://synbiohub.org/public/igem/BBa_I718004/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956310
display id: annotation1956310
version: 1
top level: BBa_I718013
location: https://synbiohub.org/public/igem/BBa_I718013/annotation1956310/range1956310/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I718013/annotation1956310/range1956310/1
type: Range
persistent identity: range1956310
display id: range1956310
version: 1
direction: direction/forward
top level: BBa_I718013
start: 89
end: 129
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956308
display id: annotation1956308
version: 1
top level: BBa_I718013
location: https://synbiohub.org/public/igem/BBa_I718013/annotation1956308/range1956308/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I718013/annotation1956308/range1956308/1
type: Range
persistent identity: range1956308
display id: range1956308
version: 1
direction: direction/forward
top level: BBa_I718013
start: 1
end: 80
orientation: inline

BBa_I718016
type: SequenceAnnotation
persistent identity: annotation1956314
display id: annotation1956314
version: 1
top level: BBa_I718013
location: https://synbiohub.org/public/igem/BBa_I718013/annotation1956314/range1956314/1
component: BBa_I718016

https://synbiohub.org/public/igem/BBa_I718013/annotation1956314/range1956314/1
type: Range
persistent identity: range1956314
display id: range1956314
version: 1
direction: direction/reverse
top level: BBa_I718013
start: 138
end: 171
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956316
display id: annotation1956316
version: 1
top level: BBa_I718013
location: https://synbiohub.org/public/igem/BBa_I718013/annotation1956316/range1956316/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718013/annotation1956316/range1956316/1
type: Range
persistent identity: range1956316
display id: range1956316
version: 1
direction: direction/forward
top level: BBa_I718013
start: 180
end: 194
orientation: inline

BBa_I718004
type: SequenceAnnotation
persistent identity: annotation1956321
display id: annotation1956321
version: 1
top level: BBa_I718013
location: https://synbiohub.org/public/igem/BBa_I718013/annotation1956321/range1956321/1
component: BBa_I718004

https://synbiohub.org/public/igem/BBa_I718013/annotation1956321/range1956321/1
type: Range
persistent identity: range1956321
display id: range1956321
version: 1
direction: direction/reverse
top level: BBa_I718013
start: 201
end: 1082
orientation: inline

BBa_K203125
type: ComponentDefinition
persistent identity: BBa K203125
display id: BBa_K203125
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SREBP regulated promoter, predicted, tested HB8
creator: Nao Iwamoto and Tim Heinemann
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _301_
m user id: 0
owner id: 5074
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: To be updated
mutable notes: To be updated
mutable provenance: To be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CMV core promoter
sequence annotation: SREBP binding site
sequence annotation: Sp1 binding site
sequence: https://synbiohub.org/public/igem/BBa_K203125_sequence/1

CMV core promoter
type: SequenceAnnotation
persistent identity: annotation2059289
display id: annotation2059289
version: 1
top level: BBa_K203125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203125/annotation2059289/range2059289/1

https://synbiohub.org/public/igem/BBa_K203125/annotation2059289/range2059289/1
type: Range
persistent identity: range2059289
display id: range2059289
version: 1
direction: direction/forward
top level: BBa_K203125
start: 498
end: 589
orientation: inline

SREBP binding site
type: SequenceAnnotation
persistent identity: annotation2059361
display id: annotation2059361
version: 1
top level: BBa_K203125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203125/annotation2059361/range2059361/1

https://synbiohub.org/public/igem/BBa_K203125/annotation2059361/range2059361/1
type: Range
persistent identity: range2059361
display id: range2059361
version: 1
direction: direction/forward
top level: BBa_K203125
start: 421
end: 431
orientation: inline

Sp1 binding site
type: SequenceAnnotation
persistent identity: annotation2059386
display id: annotation2059386
version: 1
top level: BBa_K203125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203125/annotation2059386/range2059386/1

https://synbiohub.org/public/igem/BBa_K203125/annotation2059386/range2059386/1
type: Range
persistent identity: range2059386
display id: range2059386
version: 1
direction: direction/forward
top level: BBa_K203125
start: 481
end: 493
orientation: inline

SREBP binding site
type: SequenceAnnotation
persistent identity: annotation2059360
display id: annotation2059360
version: 1
top level: BBa_K203125
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203125/annotation2059360/range2059360/1

https://synbiohub.org/public/igem/BBa_K203125/annotation2059360/range2059360/1
type: Range
persistent identity: range2059360
display id: range2059360
version: 1
direction: direction/forward
top level: BBa_K203125
start: 100
end: 110
orientation: inline

BBa_S0102
type: ComponentDefinition
persistent identity: BBa S0102
display id: BBa_S0102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0050
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0050
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0050
sequence: https://synbiohub.org/public/igem/BBa_S0102_sequence/1

BBa_C0050
type: Component
persistent identity: component939742
display id: component939742
version: 1
top level: BBa_S0102
access: public
definition: cI HK022

BBa_B0034
type: Component
persistent identity: component939727
display id: component939727
version: 1
top level: BBa_S0102
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939727
display id: annotation939727
version: 1
top level: BBa_S0102
location: https://synbiohub.org/public/igem/BBa_S0102/annotation939727/range939727/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0102/annotation939727/range939727/1
type: Range
persistent identity: range939727
display id: range939727
version: 1
direction: direction/forward
top level: BBa_S0102
start: 1
end: 12
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation939742
display id: annotation939742
version: 1
top level: BBa_S0102
location: https://synbiohub.org/public/igem/BBa_S0102/annotation939742/range939742/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_S0102/annotation939742/range939742/1
type: Range
persistent identity: range939742
display id: range939742
version: 1
direction: direction/forward
top level: BBa_S0102
start: 19
end: 762
orientation: inline

BBa_J52012
type: ComponentDefinition
persistent identity: BBa J52012
display id: BBa_J52012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Rluc-linker-PEST191
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Rluc-linker-PEST191 is a part composed of Rluc (which acts as a reporter) linked with PEST191 through six aminoacid long protein linker. PEST191 is a sequence that leads towards degradation of protein linked with this sequence.
mutable notes: Rluc-linker-PEST 191 was made by using PCR ligation technique. This part is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: PEST191
sequence annotation: linker
sequence annotation: Rluc-linker-PEST191
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52012_sequence/1

PEST191
type: SequenceAnnotation
persistent identity: annotation1902839
display id: annotation1902839
version: 1
top level: BBa_J52012
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902839/range1902839/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902839/range1902839/1
type: Range
persistent identity: range1902839
display id: range1902839
version: 1
direction: direction/forward
top level: BBa_J52012
start: 952
end: 1437
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1902838
display id: annotation1902838
version: 1
top level: BBa_J52012
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902838/range1902838/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902838/range1902838/1
type: Range
persistent identity: range1902838
display id: range1902838
version: 1
direction: direction/forward
top level: BBa_J52012
start: 934
end: 951
orientation: inline

Rluc-linker-PEST191
type: SequenceAnnotation
persistent identity: annotation1902847
display id: annotation1902847
version: 1
top level: BBa_J52012
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902847/range1902847/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902847/range1902847/1
type: Range
persistent identity: range1902847
display id: range1902847
version: 1
direction: direction/forward
top level: BBa_J52012
start: 1
end: 1437
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902837
display id: annotation1902837
version: 1
top level: BBa_J52012
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52012/annotation1902837/range1902837/1

https://synbiohub.org/public/igem/BBa_J52012/annotation1902837/range1902837/1
type: Range
persistent identity: range1902837
display id: range1902837
version: 1
direction: direction/forward
top level: BBa_J52012
start: 1
end: 933
orientation: inline

BBa_I718014
type: ComponentDefinition
persistent identity: BBa I718014
display id: BBa_I718014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-dapA (B. Subtilis)
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I718005
component: BBa_B0030
sequence annotation: BBa_I718005
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_I718014_sequence/1

BBa_I718005
type: Component
persistent identity: component1956197
display id: component1956197
version: 1
top level: BBa_I718014
access: public
definition: https://synbiohub.org/public/igem/BBa_I718005/1

BBa_B0030
type: Component
persistent identity: component1956192
display id: component1956192
version: 1
top level: BBa_I718014
access: public
definition: BBa_B0030

BBa_I718005
type: SequenceAnnotation
persistent identity: annotation1956197
display id: annotation1956197
version: 1
top level: BBa_I718014
location: https://synbiohub.org/public/igem/BBa_I718014/annotation1956197/range1956197/1
component: BBa_I718005

https://synbiohub.org/public/igem/BBa_I718014/annotation1956197/range1956197/1
type: Range
persistent identity: range1956197
display id: range1956197
version: 1
direction: direction/reverse
top level: BBa_I718014
start: 22
end: 897
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956192
display id: annotation1956192
version: 1
top level: BBa_I718014
location: https://synbiohub.org/public/igem/BBa_I718014/annotation1956192/range1956192/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718014/annotation1956192/range1956192/1
type: Range
persistent identity: range1956192
display id: range1956192
version: 1
direction: direction/forward
top level: BBa_I718014
start: 1
end: 15
orientation: inline

BBa_K084007
type: ComponentDefinition
persistent identity: BBa K084007
display id: BBa_K084007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lac repressible lasI generator (No LVA )
creator: Masahiro Tominaga,Yoshimi Iyama,Kohei Kawasaki
created: 2008-09-17T11:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _189_
m user id: 0
owner id: 1595
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Generates 3OC12-HSL under a lac-repressible promoter
mutable notes: None
mutable provenance: BBa_J04500 BBa_C0178
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_C0178
component: BBa_R0010
sequence annotation: BBa_C0178
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K084007_sequence/1

BBa_B0034
type: Component
persistent identity: component1976089
display id: component1976089
version: 1
top level: BBa_K084007
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0178
type: Component
persistent identity: component1976091
display id: component1976091
version: 1
top level: BBa_K084007
access: public
definition: https://synbiohub.org/public/igem/BBa_C0178/1

BBa_R0010
type: Component
persistent identity: component1976081
display id: component1976081
version: 1
top level: BBa_K084007
access: public
definition: LacI

BBa_C0178
type: SequenceAnnotation
persistent identity: annotation1976091
display id: annotation1976091
version: 1
top level: BBa_K084007
location: https://synbiohub.org/public/igem/BBa_K084007/annotation1976091/range1976091/1
component: BBa_C0178

https://synbiohub.org/public/igem/BBa_K084007/annotation1976091/range1976091/1
type: Range
persistent identity: range1976091
display id: range1976091
version: 1
direction: direction/reverse
top level: BBa_K084007
start: 227
end: 835
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1976081
display id: annotation1976081
version: 1
top level: BBa_K084007
location: https://synbiohub.org/public/igem/BBa_K084007/annotation1976081/range1976081/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K084007/annotation1976081/range1976081/1
type: Range
persistent identity: range1976081
display id: range1976081
version: 1
direction: direction/forward
top level: BBa_K084007
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1976089
display id: annotation1976089
version: 1
top level: BBa_K084007
location: https://synbiohub.org/public/igem/BBa_K084007/annotation1976089/range1976089/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K084007/annotation1976089/range1976089/1
type: Range
persistent identity: range1976089
display id: range1976089
version: 1
direction: direction/reverse
top level: BBa_K084007
start: 209
end: 220
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2000
display id: BBa_R2000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Zif23 regulated, test: between
creator: Ronny Krashinsky
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites.
mutable notes: consensus sequences based on [Jensen98].
mutable provenance: synthetic
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: (+1)
sequence annotation: Zif23
sequence annotation: consensus
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R2000_sequence/1

(+1)
type: SequenceAnnotation
persistent identity: annotation318102
display id: annotation318102
version: 1
top level: Zif23
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318102/range318102/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318102/range318102/1
type: Range
persistent identity: range318102
display id: range318102
version: 1
direction: direction/forward
top level: Zif23
start: 45
end: 45
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318099
display id: annotation318099
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318099/range318099/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318099/range318099/1
type: Range
persistent identity: range318099
display id: range318099
version: 1
direction: direction/forward
top level: Zif23
start: 20
end: 33
orientation: inline

consensus
type: SequenceAnnotation
persistent identity: annotation318100
display id: annotation318100
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318100/range318100/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318100/range318100/1
type: Range
persistent identity: range318100
display id: range318100
version: 1
direction: direction/forward
top level: Zif23
start: 1
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318098
display id: annotation318098
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318098/range318098/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318098/range318098/1
type: Range
persistent identity: range318098
display id: range318098
version: 1
direction: direction/forward
top level: Zif23
start: 33
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318094
display id: annotation318094
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2000/annotation318094/range318094/1

https://synbiohub.org/public/igem/BBa_R2000/annotation318094/range318094/1
type: Range
persistent identity: range318094
display id: range318094
version: 1
direction: direction/forward
top level: Zif23
start: 10
end: 15
orientation: inline

mnt (s)
type: ComponentDefinition
persistent identity: BBa C0072
display id: BBa_C0072
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt repressor (strong) from Salmonella phage P22 (+LVA)
creator: crackdots
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor Kendall, KL; Sauer, RT The Journal of Biological Chemistry, 264(23):13706-13710, 1989
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mnt
sequence annotation: LVA
sequence annotation: T
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0072_sequence/1

mnt
type: SequenceAnnotation
persistent identity: annotation308499
display id: annotation308499
version: 1
top level: mnt (s)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1

https://synbiohub.org/public/igem/BBa_C0072/annotation308499/range308499/1
type: Range
persistent identity: range308499
display id: range308499
version: 1
direction: direction/forward
top level: mnt (s)
start: 1
end: 249
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308503
display id: annotation308503
version: 1
top level: mnt (s)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1

https://synbiohub.org/public/igem/BBa_C0072/annotation308503/range308503/1
type: Range
persistent identity: range308503
display id: range308503
version: 1
direction: direction/forward
top level: mnt (s)
start: 250
end: 282
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation308502
display id: annotation308502
version: 1
top level: mnt (s)
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1

https://synbiohub.org/public/igem/BBa_C0072/annotation308502/range308502/1
type: Range
persistent identity: range308502
display id: range308502
version: 1
direction: direction/forward
top level: mnt (s)
start: 95
end: 97
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214010
display id: annotation2214010
version: 1
top level: mnt (s)
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1

https://synbiohub.org/public/igem/BBa_C0072/annotation2214010/range2214010/1
type: Range
persistent identity: range2214010
display id: range2214010
version: 1
direction: direction/forward
top level: mnt (s)
start: 289
end: 313
orientation: inline

BBa_J24018
type: ComponentDefinition
persistent identity: BBa J24018
display id: BBa_J24018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TetR promoted AHL sensor and LacI producer
creator: Jamie Lemon
created: 2006-07-05T11:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1016
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part produces LuxR when no tetR is present, which forms a complex with AHL to activate pLuxR promotor leading to LacI transcription.
mutable notes: being constructed
mutable provenance: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatcacacaggactactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgc…
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_F2620
component: BBa_P0312
sequence annotation: BBa_F2620
sequence annotation: BBa_P0312
sequence: https://synbiohub.org/public/igem/BBa_J24018_sequence/1

BBa_F2620
type: Component
persistent identity: component2278676
display id: component2278676
version: 1
top level: BBa_J24018
access: public
definition: BBa_F2620

BBa_P0312
type: Component
persistent identity: component2278690
display id: component2278690
version: 1
top level: BBa_J24018
access: public
definition: https://synbiohub.org/public/igem/BBa_P0312/1

BBa_F2620
type: SequenceAnnotation
persistent identity: annotation2278676
display id: annotation2278676
version: 1
top level: BBa_J24018
location: https://synbiohub.org/public/igem/BBa_J24018/annotation2278676/range2278676/1
component: BBa_F2620

https://synbiohub.org/public/igem/BBa_J24018/annotation2278676/range2278676/1
type: Range
persistent identity: range2278676
display id: range2278676
version: 1
direction: direction/reverse
top level: BBa_J24018
start: 1
end: 1061
orientation: inline

BBa_P0312
type: SequenceAnnotation
persistent identity: annotation2278690
display id: annotation2278690
version: 1
top level: BBa_J24018
location: https://synbiohub.org/public/igem/BBa_J24018/annotation2278690/range2278690/1
component: BBa_P0312

https://synbiohub.org/public/igem/BBa_J24018/annotation2278690/range2278690/1
type: Range
persistent identity: range2278690
display id: range2278690
version: 1
direction: direction/reverse
top level: BBa_J24018
start: 1070
end: 2376
orientation: inline

BBa_J06801
type: ComponentDefinition
persistent identity: BBa J06801
display id: BBa_J06801
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0034.J06501.B0015.R0011)
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:19Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LacI (ts) QPI w/ strong RBS
mutable notes: Mutation has been sequenced. The rest of the part will be sequenced shortly.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0010
component: BBa_R0011
component: BBa_B0012
component: BBa_J06501
component: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_J06501
sequence: https://synbiohub.org/public/igem/BBa_J06801_sequence/1

BBa_B0010
type: Component
persistent identity: component1531059
display id: component1531059
version: 1
top level: BBa_J06801
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: Component
persistent identity: component1531083
display id: component1531083
version: 1
top level: BBa_J06801
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1531069
display id: component1531069
version: 1
top level: BBa_J06801
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J06501
type: Component
persistent identity: component1531054
display id: component1531054
version: 1
top level: BBa_J06801
access: public
definition: https://synbiohub.org/public/igem/BBa_J06501/1

BBa_B0034
type: Component
persistent identity: component1531040
display id: component1531040
version: 1
top level: BBa_J06801
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1531083
display id: annotation1531083
version: 1
top level: BBa_J06801
location: https://synbiohub.org/public/igem/BBa_J06801/annotation1531083/range1531083/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J06801/annotation1531083/range1531083/1
type: Range
persistent identity: range1531083
display id: range1531083
version: 1
direction: direction/forward
top level: BBa_J06801
start: 1317
end: 1370
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1531059
display id: annotation1531059
version: 1
top level: BBa_J06801
location: https://synbiohub.org/public/igem/BBa_J06801/annotation1531059/range1531059/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06801/annotation1531059/range1531059/1
type: Range
persistent identity: range1531059
display id: range1531059
version: 1
direction: direction/forward
top level: BBa_J06801
start: 1180
end: 1259
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1531069
display id: annotation1531069
version: 1
top level: BBa_J06801
location: https://synbiohub.org/public/igem/BBa_J06801/annotation1531069/range1531069/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06801/annotation1531069/range1531069/1
type: Range
persistent identity: range1531069
display id: range1531069
version: 1
direction: direction/forward
top level: BBa_J06801
start: 1268
end: 1308
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1531040
display id: annotation1531040
version: 1
top level: BBa_J06801
location: https://synbiohub.org/public/igem/BBa_J06801/annotation1531040/range1531040/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06801/annotation1531040/range1531040/1
type: Range
persistent identity: range1531040
display id: range1531040
version: 1
direction: direction/forward
top level: BBa_J06801
start: 1
end: 12
orientation: inline

BBa_J06501
type: SequenceAnnotation
persistent identity: annotation1531054
display id: annotation1531054
version: 1
top level: BBa_J06801
location: https://synbiohub.org/public/igem/BBa_J06801/annotation1531054/range1531054/1
component: BBa_J06501

https://synbiohub.org/public/igem/BBa_J06801/annotation1531054/range1531054/1
type: Range
persistent identity: range1531054
display id: range1531054
version: 1
direction: direction/forward
top level: BBa_J06801
start: 19
end: 1171
orientation: inline

BBa_K203111
type: ComponentDefinition
persistent identity: BBa K203111
display id: BBa_K203111
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter; 2 REU
creator: Lars Velten, Simon Haas, Hannah Meyer, Anne Rademacher, Hannah Uckelmann and Corinna Hiller
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _301_
m user id: 0
owner id: 5074
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: To be updated
mutable notes: To be updated
mutable provenance: To be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: NFkB binding site
sequence annotation: CREB binding site
sequence annotation: Sp1 binding site
sequence annotation: JeT Core promoter
sequence annotation: Ap1 binding site
sequence: https://synbiohub.org/public/igem/BBa_K203111_sequence/1

NFkB binding site
type: SequenceAnnotation
persistent identity: annotation2045351
display id: annotation2045351
version: 1
top level: BBa_K203111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203111/annotation2045351/range2045351/1

https://synbiohub.org/public/igem/BBa_K203111/annotation2045351/range2045351/1
type: Range
persistent identity: range2045351
display id: range2045351
version: 1
direction: direction/forward
top level: BBa_K203111
start: 38
end: 51
orientation: inline

CREB binding site
type: SequenceAnnotation
persistent identity: annotation2045363
display id: annotation2045363
version: 1
top level: BBa_K203111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203111/annotation2045363/range2045363/1

https://synbiohub.org/public/igem/BBa_K203111/annotation2045363/range2045363/1
type: Range
persistent identity: range2045363
display id: range2045363
version: 1
direction: direction/forward
top level: BBa_K203111
start: 194
end: 207
orientation: inline

NFkB binding site
type: SequenceAnnotation
persistent identity: annotation2045364
display id: annotation2045364
version: 1
top level: BBa_K203111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203111/annotation2045364/range2045364/1

https://synbiohub.org/public/igem/BBa_K203111/annotation2045364/range2045364/1
type: Range
persistent identity: range2045364
display id: range2045364
version: 1
direction: direction/forward
top level: BBa_K203111
start: 248
end: 260
orientation: inline

CREB binding site
type: SequenceAnnotation
persistent identity: annotation2045352
display id: annotation2045352
version: 1
top level: BBa_K203111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203111/annotation2045352/range2045352/1

https://synbiohub.org/public/igem/BBa_K203111/annotation2045352/range2045352/1
type: Range
persistent identity: range2045352
display id: range2045352
version: 1
direction: direction/forward
top level: BBa_K203111
start: 90
end: 104
orientation: inline

Sp1 binding site
type: SequenceAnnotation
persistent identity: annotation2045353
display id: annotation2045353
version: 1
top level: BBa_K203111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203111/annotation2045353/range2045353/1

https://synbiohub.org/public/igem/BBa_K203111/annotation2045353/range2045353/1
type: Range
persistent identity: range2045353
display id: range2045353
version: 1
direction: direction/forward
top level: BBa_K203111
start: 154
end: 164
orientation: inline

JeT Core promoter
type: SequenceAnnotation
persistent identity: annotation2045366
display id: annotation2045366
version: 1
top level: BBa_K203111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203111/annotation2045366/range2045366/1

https://synbiohub.org/public/igem/BBa_K203111/annotation2045366/range2045366/1
type: Range
persistent identity: range2045366
display id: range2045366
version: 1
direction: direction/forward
top level: BBa_K203111
start: 325
end: 426
orientation: inline

Ap1 binding site
type: SequenceAnnotation
persistent identity: annotation2045365
display id: annotation2045365
version: 1
top level: BBa_K203111
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203111/annotation2045365/range2045365/1

https://synbiohub.org/public/igem/BBa_K203111/annotation2045365/range2045365/1
type: Range
persistent identity: range2045365
display id: range2045365
version: 1
direction: direction/forward
top level: BBa_K203111
start: 298
end: 307
orientation: inline

pTEF
type: ComponentDefinition
persistent identity: BBa K165037
display id: BBa_K165037
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TEF2 yeast constitutive promoter
creator: John Szymanski
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:10:56Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is a high level constitutive promoter from the yeast gene TEF2.
mutable notes: -
mutable provenance: From the yeast genome using promoter definition from: Mumberg, Muller, and Funk: http://dx.doi.org/10.1016/0378-1119(95)00037-7
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165037_sequence/1

BBa_K145300
type: ComponentDefinition
persistent identity: BBa K145300
display id: BBa_K145300
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lactonase controlled by key/lock
creator: Nathalie Busschaert
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device can produce a lactonase. Normally, however, de production of lactonase is inhibited by the lock. When key is added (or produced) the production of lactonase will occur.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0060
component: BBa_B0015
component: BBa_J23109
component: BBa_J23032
sequence annotation: BBa_C0060
sequence annotation: BBa_J23032
sequence annotation: BBa_B0015
sequence annotation: BBa_J23109
sequence: https://synbiohub.org/public/igem/BBa_K145300_sequence/1

BBa_C0060
type: Component
persistent identity: component2254695
display id: component2254695
version: 1
top level: BBa_K145300
access: public
definition: https://synbiohub.org/public/igem/BBa_C0060/1

BBa_B0015
type: Component
persistent identity: component2254705
display id: component2254705
version: 1
top level: BBa_K145300
access: public
definition: BBa_B0015

BBa_J23109
type: Component
persistent identity: component2254691
display id: component2254691
version: 1
top level: BBa_K145300
access: public
definition: https://synbiohub.org/public/igem/BBa_J23109/1

BBa_J23032
type: Component
persistent identity: component2254692
display id: component2254692
version: 1
top level: BBa_K145300
access: public
definition: https://synbiohub.org/public/igem/BBa_J23032/1

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation2254695
display id: annotation2254695
version: 1
top level: BBa_K145300
location: https://synbiohub.org/public/igem/BBa_K145300/annotation2254695/range2254695/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_K145300/annotation2254695/range2254695/1
type: Range
persistent identity: range2254695
display id: range2254695
version: 1
direction: direction/forward
top level: BBa_K145300
start: 93
end: 881
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation2254692
display id: annotation2254692
version: 1
top level: BBa_K145300
location: https://synbiohub.org/public/igem/BBa_K145300/annotation2254692/range2254692/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K145300/annotation2254692/range2254692/1
type: Range
persistent identity: range2254692
display id: range2254692
version: 1
direction: direction/reverse
top level: BBa_K145300
start: 44
end: 86
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2254705
display id: annotation2254705
version: 1
top level: BBa_K145300
location: https://synbiohub.org/public/igem/BBa_K145300/annotation2254705/range2254705/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K145300/annotation2254705/range2254705/1
type: Range
persistent identity: range2254705
display id: range2254705
version: 1
direction: direction/reverse
top level: BBa_K145300
start: 915
end: 1043
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2254691
display id: annotation2254691
version: 1
top level: BBa_K145300
location: https://synbiohub.org/public/igem/BBa_K145300/annotation2254691/range2254691/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K145300/annotation2254691/range2254691/1
type: Range
persistent identity: range2254691
display id: range2254691
version: 1
direction: direction/reverse
top level: BBa_K145300
start: 1
end: 35
orientation: inline

SV40 NLS
type: ComponentDefinition
persistent identity: BBa J63008
display id: BBa_J63008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SV40 nuclear localization sequence from SV40; yeast codon optimized
creator: Caroline Ajo-Franklin
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: yeast codon-optimized nuclear localization sequence from simian virus 40
mutable notes: as described in literature
mutable provenance: direct synthesis from published sequence
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_J63008_sequence/1

BBa_I718011
type: ComponentDefinition
persistent identity: BBa I718011
display id: BBa_I718011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs-dapA coli
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I718004
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_I718004
sequence: https://synbiohub.org/public/igem/BBa_I718011_sequence/1

BBa_I718004
type: Component
persistent identity: component1956162
display id: component1956162
version: 1
top level: BBa_I718011
access: public
definition: https://synbiohub.org/public/igem/BBa_I718004/1

BBa_B0030
type: Component
persistent identity: component1956157
display id: component1956157
version: 1
top level: BBa_I718011
access: public
definition: BBa_B0030

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956157
display id: annotation1956157
version: 1
top level: BBa_I718011
location: https://synbiohub.org/public/igem/BBa_I718011/annotation1956157/range1956157/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718011/annotation1956157/range1956157/1
type: Range
persistent identity: range1956157
display id: range1956157
version: 1
direction: direction/forward
top level: BBa_I718011
start: 1
end: 15
orientation: inline

BBa_I718004
type: SequenceAnnotation
persistent identity: annotation1956162
display id: annotation1956162
version: 1
top level: BBa_I718011
location: https://synbiohub.org/public/igem/BBa_I718011/annotation1956162/range1956162/1
component: BBa_I718004

https://synbiohub.org/public/igem/BBa_I718011/annotation1956162/range1956162/1
type: Range
persistent identity: range1956162
display id: range1956162
version: 1
direction: direction/reverse
top level: BBa_I718011
start: 22
end: 903
orientation: inline

BBa_I732074
type: ComponentDefinition
persistent identity: BBa I732074
display id: BBa_I732074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Adaptor + LacZ-alpha Reporter
creator: Zhan Jian
created: 2007-08-01T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_I732006
component: BBa_I732007
sequence annotation: BBa_I732006
sequence annotation: BBa_B0034
sequence annotation: BBa_I732007
sequence: https://synbiohub.org/public/igem/BBa_I732074_sequence/1

BBa_B0034
type: Component
persistent identity: component1940656
display id: component1940656
version: 1
top level: BBa_I732074
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732006
type: Component
persistent identity: component1940660
display id: component1940660
version: 1
top level: BBa_I732074
access: public
definition: lacZ-alpha

BBa_I732007
type: Component
persistent identity: component1940654
display id: component1940654
version: 1
top level: BBa_I732074
access: public
definition: Adapter

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1940660
display id: annotation1940660
version: 1
top level: BBa_I732074
location: https://synbiohub.org/public/igem/BBa_I732074/annotation1940660/range1940660/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732074/annotation1940660/range1940660/1
type: Range
persistent identity: range1940660
display id: range1940660
version: 1
direction: direction/reverse
top level: BBa_I732074
start: 46
end: 279
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940656
display id: annotation1940656
version: 1
top level: BBa_I732074
location: https://synbiohub.org/public/igem/BBa_I732074/annotation1940656/range1940656/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732074/annotation1940656/range1940656/1
type: Range
persistent identity: range1940656
display id: range1940656
version: 1
direction: direction/reverse
top level: BBa_I732074
start: 28
end: 39
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1940654
display id: annotation1940654
version: 1
top level: BBa_I732074
location: https://synbiohub.org/public/igem/BBa_I732074/annotation1940654/range1940654/1
component: BBa_I732007

https://synbiohub.org/public/igem/BBa_I732074/annotation1940654/range1940654/1
type: Range
persistent identity: range1940654
display id: range1940654
version: 1
direction: direction/reverse
top level: BBa_I732074
start: 1
end: 19
orientation: inline

BBa_K081012
type: ComponentDefinition
persistent identity: BBa K081012
display id: BBa_K081012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP protein generator - PoPS->GFP
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: PoPS in -> GFP out Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: GFP: T:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_E0040
component: BBa_B1006
component: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081012_sequence/1

BBa_E0040
type: Component
persistent identity: component1981957
display id: component1981957
version: 1
top level: BBa_K081012
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B1006
type: Component
persistent identity: component1981962
display id: component1981962
version: 1
top level: BBa_K081012
access: public
definition: BBa_B1006

BBa_B0030
type: Component
persistent identity: component1981954
display id: component1981954
version: 1
top level: BBa_K081012
access: public
definition: BBa_B0030

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1981957
display id: annotation1981957
version: 1
top level: BBa_K081012
location: https://synbiohub.org/public/igem/BBa_K081012/annotation1981957/range1981957/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K081012/annotation1981957/range1981957/1
type: Range
persistent identity: range1981957
display id: range1981957
version: 1
direction: direction/reverse
top level: BBa_K081012
start: 22
end: 741
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1981954
display id: annotation1981954
version: 1
top level: BBa_K081012
location: https://synbiohub.org/public/igem/BBa_K081012/annotation1981954/range1981954/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081012/annotation1981954/range1981954/1
type: Range
persistent identity: range1981954
display id: range1981954
version: 1
direction: direction/forward
top level: BBa_K081012
start: 1
end: 15
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation1981962
display id: annotation1981962
version: 1
top level: BBa_K081012
location: https://synbiohub.org/public/igem/BBa_K081012/annotation1981962/range1981962/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081012/annotation1981962/range1981962/1
type: Range
persistent identity: range1981962
display id: range1981962
version: 1
direction: direction/reverse
top level: BBa_K081012
start: 750
end: 788
orientation: inline

BBa_I746009
type: ComponentDefinition
persistent identity: BBa I746009
display id: BBa_I746009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: AIP generator + outer membrane permeability
creator: David Wyatt
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 1851
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate - consists of AIP generator (I746000) + term + outer membrane porosity (using the mutant FepA device).
mutable notes: N/A
mutable provenance: Standard assembly
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23111
component: BBa_I746200
component: BBa_I746000
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I746200
sequence annotation: BBa_I746000
sequence annotation: BBa_B0034
sequence annotation: BBa_J23111
sequence: https://synbiohub.org/public/igem/BBa_I746009_sequence/1

BBa_J23111
type: Component
persistent identity: component1953283
display id: component1953283
version: 1
top level: BBa_I746009
access: public
definition: https://synbiohub.org/public/igem/BBa_J23111/1

BBa_I746200
type: Component
persistent identity: component1953286
display id: component1953286
version: 1
top level: BBa_I746009
access: public
definition: https://synbiohub.org/public/igem/BBa_I746200/1

BBa_I746000
type: Component
persistent identity: component1953276
display id: component1953276
version: 1
top level: BBa_I746009
access: public
definition: https://synbiohub.org/public/igem/BBa_I746000/1

BBa_B0010
type: Component
persistent identity: component1953287
display id: component1953287
version: 1
top level: BBa_I746009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1953277
display id: component1953277
version: 1
top level: BBa_I746009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1953285
display id: component1953285
version: 1
top level: BBa_I746009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1953289
display id: component1953289
version: 1
top level: BBa_I746009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1953279
display id: component1953279
version: 1
top level: BBa_I746009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1953279
display id: annotation1953279
version: 1
top level: BBa_I746009
location: https://synbiohub.org/public/igem/BBa_I746009/annotation1953279/range1953279/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746009/annotation1953279/range1953279/1
type: Range
persistent identity: range1953279
display id: range1953279
version: 1
direction: direction/forward
top level: BBa_I746009
start: 856
end: 896
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1953277
display id: annotation1953277
version: 1
top level: BBa_I746009
location: https://synbiohub.org/public/igem/BBa_I746009/annotation1953277/range1953277/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746009/annotation1953277/range1953277/1
type: Range
persistent identity: range1953277
display id: range1953277
version: 1
direction: direction/forward
top level: BBa_I746009
start: 768
end: 847
orientation: inline

BBa_I746200
type: SequenceAnnotation
persistent identity: annotation1953286
display id: annotation1953286
version: 1
top level: BBa_I746009
location: https://synbiohub.org/public/igem/BBa_I746009/annotation1953286/range1953286/1
component: BBa_I746200

https://synbiohub.org/public/igem/BBa_I746009/annotation1953286/range1953286/1
type: Range
persistent identity: range1953286
display id: range1953286
version: 1
direction: direction/reverse
top level: BBa_I746009
start: 966
end: 3173
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1953289
display id: annotation1953289
version: 1
top level: BBa_I746009
location: https://synbiohub.org/public/igem/BBa_I746009/annotation1953289/range1953289/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746009/annotation1953289/range1953289/1
type: Range
persistent identity: range1953289
display id: range1953289
version: 1
direction: direction/forward
top level: BBa_I746009
start: 3270
end: 3310
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1953287
display id: annotation1953287
version: 1
top level: BBa_I746009
location: https://synbiohub.org/public/igem/BBa_I746009/annotation1953287/range1953287/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746009/annotation1953287/range1953287/1
type: Range
persistent identity: range1953287
display id: range1953287
version: 1
direction: direction/forward
top level: BBa_I746009
start: 3182
end: 3261
orientation: inline

BBa_I746000
type: SequenceAnnotation
persistent identity: annotation1953276
display id: annotation1953276
version: 1
top level: BBa_I746009
location: https://synbiohub.org/public/igem/BBa_I746009/annotation1953276/range1953276/1
component: BBa_I746000

https://synbiohub.org/public/igem/BBa_I746009/annotation1953276/range1953276/1
type: Range
persistent identity: range1953276
display id: range1953276
version: 1
direction: direction/reverse
top level: BBa_I746009
start: 1
end: 759
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1953285
display id: annotation1953285
version: 1
top level: BBa_I746009
location: https://synbiohub.org/public/igem/BBa_I746009/annotation1953285/range1953285/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746009/annotation1953285/range1953285/1
type: Range
persistent identity: range1953285
display id: range1953285
version: 1
direction: direction/reverse
top level: BBa_I746009
start: 948
end: 959
orientation: inline

BBa_J23111
type: SequenceAnnotation
persistent identity: annotation1953283
display id: annotation1953283
version: 1
top level: BBa_I746009
location: https://synbiohub.org/public/igem/BBa_I746009/annotation1953283/range1953283/1
component: BBa_J23111

https://synbiohub.org/public/igem/BBa_I746009/annotation1953283/range1953283/1
type: Range
persistent identity: range1953283
display id: range1953283
version: 1
direction: direction/reverse
top level: BBa_I746009
start: 905
end: 939
orientation: inline

BBa_I13600
type: ComponentDefinition
persistent identity: BBa I13600
display id: BBa_I13600
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet with CFP reporter (without LVA tag)
creator: Christopher Batten, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_B0012
component: BBa_B0034
component: BBa_E0020
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I13600_sequence/1

BBa_R0040
type: Component
persistent identity: component947184
display id: component947184
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component947209
display id: component947209
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component947192
display id: component947192
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: Component
persistent identity: component947194
display id: component947194
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component947199
display id: component947199
version: 1
top level: BBa_I13600
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation947192
display id: annotation947192
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947192/range947192/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13600/annotation947192/range947192/1
type: Range
persistent identity: range947192
display id: range947192
version: 1
direction: direction/forward
top level: BBa_I13600
start: 63
end: 74
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation947194
display id: annotation947194
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947194/range947194/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13600/annotation947194/range947194/1
type: Range
persistent identity: range947194
display id: range947194
version: 1
direction: direction/forward
top level: BBa_I13600
start: 81
end: 803
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation947209
display id: annotation947209
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947209/range947209/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13600/annotation947209/range947209/1
type: Range
persistent identity: range947209
display id: range947209
version: 1
direction: direction/forward
top level: BBa_I13600
start: 900
end: 940
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation947199
display id: annotation947199
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947199/range947199/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13600/annotation947199/range947199/1
type: Range
persistent identity: range947199
display id: range947199
version: 1
direction: direction/forward
top level: BBa_I13600
start: 812
end: 891
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation947184
display id: annotation947184
version: 1
top level: BBa_I13600
location: https://synbiohub.org/public/igem/BBa_I13600/annotation947184/range947184/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13600/annotation947184/range947184/1
type: Range
persistent identity: range947184
display id: range947184
version: 1
direction: direction/forward
top level: BBa_I13600
start: 1
end: 54
orientation: inline

BBa_K081024
type: ComponentDefinition
persistent identity: BBa K081024
display id: BBa_K081024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcyA protein generator and Pomp
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device is a PoPS in -> pcyA out with Pomp promoter downstream. Pomp (BBa_R0082) is a positively regulated, OmpR-controlled promoter. Phosphorylated OmpR binds to the three operator sites and activates transcription. Proteins pcyA, ho1 and cph8 can produce a photosensitive complex that can activate the endogenous transcription factor OmpR. So, light (in particular 660 nm radiation) is an indirect repressor of Pomp. These interactions are valid if used in E.coli deficient in natural EnvZ, because EnvZ can activate OmpR. {|align="center" |}
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: pcyA: T: Pomp:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_R0082
component: BBa_I15009
component: BBa_B1006
sequence annotation: BBa_B0030
sequence annotation: BBa_R0082
sequence annotation: BBa_I15009
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081024_sequence/1

BBa_B0030
type: Component
persistent identity: component2249322
display id: component2249322
version: 1
top level: BBa_K081024
access: public
definition: BBa_B0030

BBa_R0082
type: Component
persistent identity: component2249337
display id: component2249337
version: 1
top level: BBa_K081024
access: public
definition: OmpR

BBa_I15009
type: Component
persistent identity: component2249326
display id: component2249326
version: 1
top level: BBa_K081024
access: public
definition: BBa_I15009

BBa_B1006
type: Component
persistent identity: component2249331
display id: component2249331
version: 1
top level: BBa_K081024
access: public
definition: BBa_B1006

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249322
display id: annotation2249322
version: 1
top level: BBa_K081024
location: https://synbiohub.org/public/igem/BBa_K081024/annotation2249322/range2249322/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081024/annotation2249322/range2249322/1
type: Range
persistent identity: range2249322
display id: range2249322
version: 1
direction: direction/forward
top level: BBa_K081024
start: 1
end: 15
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2249337
display id: annotation2249337
version: 1
top level: BBa_K081024
location: https://synbiohub.org/public/igem/BBa_K081024/annotation2249337/range2249337/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K081024/annotation2249337/range2249337/1
type: Range
persistent identity: range2249337
display id: range2249337
version: 1
direction: direction/reverse
top level: BBa_K081024
start: 852
end: 959
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2249326
display id: annotation2249326
version: 1
top level: BBa_K081024
location: https://synbiohub.org/public/igem/BBa_K081024/annotation2249326/range2249326/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K081024/annotation2249326/range2249326/1
type: Range
persistent identity: range2249326
display id: range2249326
version: 1
direction: direction/reverse
top level: BBa_K081024
start: 22
end: 796
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2249331
display id: annotation2249331
version: 1
top level: BBa_K081024
location: https://synbiohub.org/public/igem/BBa_K081024/annotation2249331/range2249331/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081024/annotation2249331/range2249331/1
type: Range
persistent identity: range2249331
display id: range2249331
version: 1
direction: direction/reverse
top level: BBa_K081024
start: 805
end: 843
orientation: inline

BBa_K081014
type: ComponentDefinition
persistent identity: BBa K081014
display id: BBa_K081014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RFP protein generator - PoPS->RFP
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: PoPS in -> RFP out Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: RFP: T:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_E1010
component: BBa_B0030
component: BBa_B1006
sequence annotation: BBa_E1010
sequence annotation: BBa_B0030
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081014_sequence/1

BBa_E1010
type: Component
persistent identity: component2246150
display id: component2246150
version: 1
top level: BBa_K081014
access: public
definition: mRFP1

BBa_B0030
type: Component
persistent identity: component2246146
display id: component2246146
version: 1
top level: BBa_K081014
access: public
definition: BBa_B0030

BBa_B1006
type: Component
persistent identity: component2246155
display id: component2246155
version: 1
top level: BBa_K081014
access: public
definition: BBa_B1006

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2246150
display id: annotation2246150
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246150/range2246150/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K081014/annotation2246150/range2246150/1
type: Range
persistent identity: range2246150
display id: range2246150
version: 1
direction: direction/reverse
top level: BBa_K081014
start: 22
end: 727
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246146
display id: annotation2246146
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246146/range2246146/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081014/annotation2246146/range2246146/1
type: Range
persistent identity: range2246146
display id: range2246146
version: 1
direction: direction/forward
top level: BBa_K081014
start: 1
end: 15
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2246155
display id: annotation2246155
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246155/range2246155/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081014/annotation2246155/range2246155/1
type: Range
persistent identity: range2246155
display id: range2246155
version: 1
direction: direction/reverse
top level: BBa_K081014
start: 736
end: 774
orientation: inline

Pr + GFP
type: ComponentDefinition
persistent identity: BBa S03335
display id: BBa_S03335
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pr with GFP as a reporter
creator: ETH Zurich iGEM Team 2005
created: 2005-09-20T11:00:00Z
modified: 2015-05-08T01:14:21Z
discontinued: false
dominant: true
experience: experience/None
group u list: _34_
m user id: 0
owner id: 398
owning group id: 34
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Pr promoter of the lambda-system combined with GFP as a quantification and debugging device for the INPUT-module of the ETHZ05 iGEM team.
mutable notes: For more details see Sensing Device and Event Processing Device documentation.
mutable provenance: Registry 7.05
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_R0051
component: BBa_B0012
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_S03335_sequence/1

BBa_B0010
type: Component
persistent identity: component1700226
display id: component1700226
version: 1
top level: Pr + GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0051
type: Component
persistent identity: component1700211
display id: component1700211
version: 1
top level: Pr + GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0012
type: Component
persistent identity: component1700236
display id: component1700236
version: 1
top level: Pr + GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1700219
display id: component1700219
version: 1
top level: Pr + GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1700221
display id: component1700221
version: 1
top level: Pr + GFP
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1700219
display id: annotation1700219
version: 1
top level: Pr + GFP
location: https://synbiohub.org/public/igem/BBa_S03335/annotation1700219/range1700219/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03335/annotation1700219/range1700219/1
type: Range
persistent identity: range1700219
display id: range1700219
version: 1
direction: direction/forward
top level: Pr + GFP
start: 58
end: 69
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1700236
display id: annotation1700236
version: 1
top level: Pr + GFP
location: https://synbiohub.org/public/igem/BBa_S03335/annotation1700236/range1700236/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03335/annotation1700236/range1700236/1
type: Range
persistent identity: range1700236
display id: range1700236
version: 1
direction: direction/forward
top level: Pr + GFP
start: 892
end: 932
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1700211
display id: annotation1700211
version: 1
top level: Pr + GFP
location: https://synbiohub.org/public/igem/BBa_S03335/annotation1700211/range1700211/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_S03335/annotation1700211/range1700211/1
type: Range
persistent identity: range1700211
display id: range1700211
version: 1
direction: direction/forward
top level: Pr + GFP
start: 1
end: 49
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1700226
display id: annotation1700226
version: 1
top level: Pr + GFP
location: https://synbiohub.org/public/igem/BBa_S03335/annotation1700226/range1700226/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03335/annotation1700226/range1700226/1
type: Range
persistent identity: range1700226
display id: range1700226
version: 1
direction: direction/forward
top level: Pr + GFP
start: 804
end: 883
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1700221
display id: annotation1700221
version: 1
top level: Pr + GFP
location: https://synbiohub.org/public/igem/BBa_S03335/annotation1700221/range1700221/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S03335/annotation1700221/range1700221/1
type: Range
persistent identity: range1700221
display id: range1700221
version: 1
direction: direction/forward
top level: Pr + GFP
start: 76
end: 795
orientation: inline

BBa_J06800
type: ComponentDefinition
persistent identity: BBa J06800
display id: BBa_J06800
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0034.J06500.B0015.R0011)
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LacI (ts) QPI w/ strong RBS
mutable notes: Mutation has been sequenced. The rest of the part will be sequenced shortly.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0034
component: BBa_B0010
component: BBa_R0011
component: BBa_B0012
component: BBa_J06500
sequence annotation: BBa_B0012
sequence annotation: BBa_J06500
sequence annotation: BBa_B0010
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J06800_sequence/1

BBa_B0034
type: Component
persistent identity: component1531229
display id: component1531229
version: 1
top level: BBa_J06800
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1531248
display id: component1531248
version: 1
top level: BBa_J06800
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0011
type: Component
persistent identity: component1531272
display id: component1531272
version: 1
top level: BBa_J06800
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0012
type: Component
persistent identity: component1531258
display id: component1531258
version: 1
top level: BBa_J06800
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J06500
type: Component
persistent identity: component1531243
display id: component1531243
version: 1
top level: BBa_J06800
access: public
definition: https://synbiohub.org/public/igem/BBa_J06500/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1531258
display id: annotation1531258
version: 1
top level: BBa_J06800
location: https://synbiohub.org/public/igem/BBa_J06800/annotation1531258/range1531258/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06800/annotation1531258/range1531258/1
type: Range
persistent identity: range1531258
display id: range1531258
version: 1
direction: direction/forward
top level: BBa_J06800
start: 1268
end: 1308
orientation: inline

BBa_J06500
type: SequenceAnnotation
persistent identity: annotation1531243
display id: annotation1531243
version: 1
top level: BBa_J06800
location: https://synbiohub.org/public/igem/BBa_J06800/annotation1531243/range1531243/1
component: BBa_J06500

https://synbiohub.org/public/igem/BBa_J06800/annotation1531243/range1531243/1
type: Range
persistent identity: range1531243
display id: range1531243
version: 1
direction: direction/forward
top level: BBa_J06800
start: 19
end: 1171
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1531248
display id: annotation1531248
version: 1
top level: BBa_J06800
location: https://synbiohub.org/public/igem/BBa_J06800/annotation1531248/range1531248/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06800/annotation1531248/range1531248/1
type: Range
persistent identity: range1531248
display id: range1531248
version: 1
direction: direction/forward
top level: BBa_J06800
start: 1180
end: 1259
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1531272
display id: annotation1531272
version: 1
top level: BBa_J06800
location: https://synbiohub.org/public/igem/BBa_J06800/annotation1531272/range1531272/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J06800/annotation1531272/range1531272/1
type: Range
persistent identity: range1531272
display id: range1531272
version: 1
direction: direction/forward
top level: BBa_J06800
start: 1317
end: 1370
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1531229
display id: annotation1531229
version: 1
top level: BBa_J06800
location: https://synbiohub.org/public/igem/BBa_J06800/annotation1531229/range1531229/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06800/annotation1531229/range1531229/1
type: Range
persistent identity: range1531229
display id: range1531229
version: 1
direction: direction/forward
top level: BBa_J06800
start: 1
end: 12
orientation: inline

BBa_K203109
type: ComponentDefinition
persistent identity: BBa K203109
display id: BBa_K203109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter; 2.5 REU
creator: Lars Velten, Simon Haas, Hannah Meyer, Anne Rademacher, Hannah Uckelmann and Corinna Hiller
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _301_
m user id: 0
owner id: 5074
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: To be updated
mutable notes: To be updated
mutable provenance: To be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: NFkB Binding Site
sequence annotation: Sp1 Binding Site
sequence annotation: CREB Binding Site
sequence annotation: JeT core promoter
sequence: https://synbiohub.org/public/igem/BBa_K203109_sequence/1

NFkB Binding Site
type: SequenceAnnotation
persistent identity: annotation2045455
display id: annotation2045455
version: 1
top level: BBa_K203109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203109/annotation2045455/range2045455/1

https://synbiohub.org/public/igem/BBa_K203109/annotation2045455/range2045455/1
type: Range
persistent identity: range2045455
display id: range2045455
version: 1
direction: direction/forward
top level: BBa_K203109
start: 144
end: 158
orientation: inline

Sp1 Binding Site
type: SequenceAnnotation
persistent identity: annotation2045454
display id: annotation2045454
version: 1
top level: BBa_K203109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203109/annotation2045454/range2045454/1

https://synbiohub.org/public/igem/BBa_K203109/annotation2045454/range2045454/1
type: Range
persistent identity: range2045454
display id: range2045454
version: 1
direction: direction/forward
top level: BBa_K203109
start: 93
end: 103
orientation: inline

CREB Binding Site
type: SequenceAnnotation
persistent identity: annotation2045449
display id: annotation2045449
version: 1
top level: BBa_K203109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203109/annotation2045449/range2045449/1

https://synbiohub.org/public/igem/BBa_K203109/annotation2045449/range2045449/1
type: Range
persistent identity: range2045449
display id: range2045449
version: 1
direction: direction/forward
top level: BBa_K203109
start: 44
end: 58
orientation: inline

JeT core promoter
type: SequenceAnnotation
persistent identity: annotation2045461
display id: annotation2045461
version: 1
top level: BBa_K203109
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203109/annotation2045461/range2045461/1

https://synbiohub.org/public/igem/BBa_K203109/annotation2045461/range2045461/1
type: Range
persistent identity: range2045461
display id: range2045461
version: 1
direction: direction/forward
top level: BBa_K203109
start: 176
end: 277
orientation: inline

BBa_I13401
type: ComponentDefinition
persistent identity: BBa I13401
display id: BBa_I13401
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP reporter for RHS of library test constructs
creator: jasonk
created: 2005-04-05T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 2
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part will be suffixed to a promoter.RBS library. The purpose of the experiment is to make a first pass at a library-based construction step to identify efficiency issues and also to examine maxPOPS/RIPS level.
mutable notes: This part was built by Jen as an intermediate to their nuts and bolts parts.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13401_sequence/1

BBa_B0010
type: Component
persistent identity: component1476718
display id: component1476718
version: 1
top level: BBa_I13401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1476713
display id: component1476713
version: 1
top level: BBa_I13401
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1476728
display id: component1476728
version: 1
top level: BBa_I13401
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1476728
display id: annotation1476728
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476728/range1476728/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13401/annotation1476728/range1476728/1
type: Range
persistent identity: range1476728
display id: range1476728
version: 1
direction: direction/forward
top level: BBa_I13401
start: 817
end: 857
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1476713
display id: annotation1476713
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476713/range1476713/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13401/annotation1476713/range1476713/1
type: Range
persistent identity: range1476713
display id: range1476713
version: 1
direction: direction/forward
top level: BBa_I13401
start: 1
end: 720
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1476718
display id: annotation1476718
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476718/range1476718/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13401/annotation1476718/range1476718/1
type: Range
persistent identity: range1476718
display id: range1476718
version: 1
direction: direction/forward
top level: BBa_I13401
start: 729
end: 808
orientation: inline

BBa_I715032
type: ComponentDefinition
persistent identity: BBa I715032
display id: BBa_I715032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacIq reverse
creator: Jordan Baumgardner
created: 2007-07-17T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/None
group u list: _120_
m user id: 0
owner id: 1518
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a reverse version of BBa_C0012, the lacIq repressor cds.
mutable notes: Standard biobrick prefix and suffix were used. Since PCR was used, DNA sequence verification was needed - no mutations were found.
mutable provenance: PCR was used to amplify C0012 using primers that switched the biobrick prefix and suffix.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I715032_sequence/1

BBa_S03877
type: ComponentDefinition
persistent identity: BBa S03877
display id: BBa_S03877
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:I15008
creator: Kathy Lam
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: false
group u list: _82_
m user id: 0
owner id: 927
owning group id: 82
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_I15008
sequence annotation: BBa_B0034
sequence annotation: BBa_I15008
sequence: https://synbiohub.org/public/igem/BBa_S03877_sequence/1

BBa_B0034
type: Component
persistent identity: component2243951
display id: component2243951
version: 1
top level: BBa_S03877
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I15008
type: Component
persistent identity: component2243953
display id: component2243953
version: 1
top level: BBa_S03877
access: public
definition: BBa_I15008

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243951
display id: annotation2243951
version: 1
top level: BBa_S03877
location: https://synbiohub.org/public/igem/BBa_S03877/annotation2243951/range2243951/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03877/annotation2243951/range2243951/1
type: Range
persistent identity: range2243951
display id: range2243951
version: 1
direction: direction/reverse
top level: BBa_S03877
start: 1
end: 12
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2243953
display id: annotation2243953
version: 1
top level: BBa_S03877
location: https://synbiohub.org/public/igem/BBa_S03877/annotation2243953/range2243953/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_S03877/annotation2243953/range2243953/1
type: Range
persistent identity: range2243953
display id: range2243953
version: 1
direction: direction/reverse
top level: BBa_S03877
start: 19
end: 769
orientation: inline

BBa_I13606
type: ComponentDefinition
persistent identity: BBa I13606
display id: BBa_I13606
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reporter Construct (Ty+Lc+)
creator: Chris Batton, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reporter Construct with Tet operator+YFP(LVA+) reporter followed by a Lac operator +CFP(LVA+) reporter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0011
component: BBa_E0422
component: BBa_I6062
sequence annotation: BBa_R0011
sequence annotation: BBa_E0422
sequence annotation: BBa_I6062
sequence: https://synbiohub.org/public/igem/BBa_I13606_sequence/1

BBa_R0011
type: Component
persistent identity: component2228675
display id: component2228675
version: 1
top level: BBa_I13606
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_E0422
type: Component
persistent identity: component2228693
display id: component2228693
version: 1
top level: BBa_I13606
access: public
definition: https://synbiohub.org/public/igem/BBa_E0422/1

BBa_I6062
type: Component
persistent identity: component2228674
display id: component2228674
version: 1
top level: BBa_I13606
access: public
definition: https://synbiohub.org/public/igem/BBa_I6062/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2228675
display id: annotation2228675
version: 1
top level: BBa_I13606
location: https://synbiohub.org/public/igem/BBa_I13606/annotation2228675/range2228675/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13606/annotation2228675/range2228675/1
type: Range
persistent identity: range2228675
display id: range2228675
version: 1
direction: direction/forward
top level: BBa_I13606
start: 988
end: 1041
orientation: inline

BBa_E0422
type: SequenceAnnotation
persistent identity: annotation2228693
display id: annotation2228693
version: 1
top level: BBa_I13606
location: https://synbiohub.org/public/igem/BBa_I13606/annotation2228693/range2228693/1
component: BBa_E0422

https://synbiohub.org/public/igem/BBa_I13606/annotation2228693/range2228693/1
type: Range
persistent identity: range2228693
display id: range2228693
version: 1
direction: direction/reverse
top level: BBa_I13606
start: 1051
end: 1967
orientation: inline

BBa_I6062
type: SequenceAnnotation
persistent identity: annotation2228674
display id: annotation2228674
version: 1
top level: BBa_I13606
location: https://synbiohub.org/public/igem/BBa_I13606/annotation2228674/range2228674/1
component: BBa_I6062

https://synbiohub.org/public/igem/BBa_I13606/annotation2228674/range2228674/1
type: Range
persistent identity: range2228674
display id: range2228674
version: 1
direction: direction/reverse
top level: BBa_I13606
start: 1
end: 979
orientation: inline

BBa_K203110
type: ComponentDefinition
persistent identity: BBa K203110
display id: BBa_K203110
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter; 0.4 REU
creator: Lars Velten, Simon Haas, Hannah Meyer, Anne Rademacher, Hannah Uckelmann and Corinna Hiller
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _301_
m user id: 0
owner id: 5074
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: To be updated
mutable notes: To be updated
mutable provenance: To be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Ap1 binding site
sequence annotation: Sp1 binding site
sequence annotation: JeT Core promoter
sequence: https://synbiohub.org/public/igem/BBa_K203110_sequence/1

Ap1 binding site
type: SequenceAnnotation
persistent identity: annotation2045260
display id: annotation2045260
version: 1
top level: BBa_K203110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203110/annotation2045260/range2045260/1

https://synbiohub.org/public/igem/BBa_K203110/annotation2045260/range2045260/1
type: Range
persistent identity: range2045260
display id: range2045260
version: 1
direction: direction/forward
top level: BBa_K203110
start: 90
end: 98
orientation: inline

Sp1 binding site
type: SequenceAnnotation
persistent identity: annotation2045259
display id: annotation2045259
version: 1
top level: BBa_K203110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203110/annotation2045259/range2045259/1

https://synbiohub.org/public/igem/BBa_K203110/annotation2045259/range2045259/1
type: Range
persistent identity: range2045259
display id: range2045259
version: 1
direction: direction/forward
top level: BBa_K203110
start: 38
end: 51
orientation: inline

JeT Core promoter
type: SequenceAnnotation
persistent identity: annotation2045335
display id: annotation2045335
version: 1
top level: BBa_K203110
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203110/annotation2045335/range2045335/1

https://synbiohub.org/public/igem/BBa_K203110/annotation2045335/range2045335/1
type: Range
persistent identity: range2045335
display id: range2045335
version: 1
direction: direction/forward
top level: BBa_K203110
start: 117
end: 218
orientation: inline

BBa_I13512
type: ComponentDefinition
persistent identity: BBa I13512
display id: BBa_I13512
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid 1
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The first screening plasmid. Ends will be capped with I13450. Cutting I13452 generates an internal BioBrick site.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I0500
component: BBa_B0032
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
component: BBa_E1010
component: BBa_I13452
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0032
sequence annotation: BBa_B0010
sequence annotation: BBa_I13452
sequence annotation: BBa_E1010
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I13512_sequence/1

BBa_I0500
type: Component
persistent identity: component1505241
display id: component1505241
version: 1
top level: BBa_I13512
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0032
type: Component
persistent identity: component1505249
display id: component1505249
version: 1
top level: BBa_I13512
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
persistent identity: component1505288
display id: component1505288
version: 1
top level: BBa_I13512
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1505283
display id: component1505283
version: 1
top level: BBa_I13512
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1505298
display id: component1505298
version: 1
top level: BBa_I13512
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1505257
display id: component1505257
version: 1
top level: BBa_I13512
access: public
definition: mRFP1

BBa_B0032
type: Component
persistent identity: component1505280
display id: component1505280
version: 1
top level: BBa_I13512
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_I13452
type: Component
persistent identity: component1505272
display id: component1505272
version: 1
top level: BBa_I13512
access: public
definition: BBa_I13452

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505298
display id: annotation1505298
version: 1
top level: BBa_I13512
location: https://synbiohub.org/public/igem/BBa_I13512/annotation1505298/range1505298/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13512/annotation1505298/range1505298/1
type: Range
persistent identity: range1505298
display id: range1505298
version: 1
direction: direction/forward
top level: BBa_I13512
start: 2847
end: 2887
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505283
display id: annotation1505283
version: 1
top level: BBa_I13512
location: https://synbiohub.org/public/igem/BBa_I13512/annotation1505283/range1505283/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13512/annotation1505283/range1505283/1
type: Range
persistent identity: range1505283
display id: range1505283
version: 1
direction: direction/forward
top level: BBa_I13512
start: 2031
end: 2750
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1505280
display id: annotation1505280
version: 1
top level: BBa_I13512
location: https://synbiohub.org/public/igem/BBa_I13512/annotation1505280/range1505280/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13512/annotation1505280/range1505280/1
type: Range
persistent identity: range1505280
display id: range1505280
version: 1
direction: direction/forward
top level: BBa_I13512
start: 2012
end: 2024
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505288
display id: annotation1505288
version: 1
top level: BBa_I13512
location: https://synbiohub.org/public/igem/BBa_I13512/annotation1505288/range1505288/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13512/annotation1505288/range1505288/1
type: Range
persistent identity: range1505288
display id: range1505288
version: 1
direction: direction/forward
top level: BBa_I13512
start: 2759
end: 2838
orientation: inline

BBa_I13452
type: SequenceAnnotation
persistent identity: annotation1505272
display id: annotation1505272
version: 1
top level: BBa_I13512
location: https://synbiohub.org/public/igem/BBa_I13512/annotation1505272/range1505272/1
component: BBa_I13452

https://synbiohub.org/public/igem/BBa_I13512/annotation1505272/range1505272/1
type: Range
persistent identity: range1505272
display id: range1505272
version: 1
direction: direction/forward
top level: BBa_I13512
start: 1952
end: 2003
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1505249
display id: annotation1505249
version: 1
top level: BBa_I13512
location: https://synbiohub.org/public/igem/BBa_I13512/annotation1505249/range1505249/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13512/annotation1505249/range1505249/1
type: Range
persistent identity: range1505249
display id: range1505249
version: 1
direction: direction/forward
top level: BBa_I13512
start: 1219
end: 1231
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505257
display id: annotation1505257
version: 1
top level: BBa_I13512
location: https://synbiohub.org/public/igem/BBa_I13512/annotation1505257/range1505257/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13512/annotation1505257/range1505257/1
type: Range
persistent identity: range1505257
display id: range1505257
version: 1
direction: direction/forward
top level: BBa_I13512
start: 1238
end: 1918
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1505241
display id: annotation1505241
version: 1
top level: BBa_I13512
location: https://synbiohub.org/public/igem/BBa_I13512/annotation1505241/range1505241/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13512/annotation1505241/range1505241/1
type: Range
persistent identity: range1505241
display id: range1505241
version: 1
direction: direction/forward
top level: BBa_I13512
start: 1
end: 1210
orientation: inline

BBa_S03518
type: ComponentDefinition
persistent identity: BBa S03518
display id: BBa_S03518
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:C0040
creator: Azeem Kaka
created: 2006-07-23T11:00:00Z
modified: 2015-05-08T01:14:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1061
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0040
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_S03518_sequence/1

BBa_C0040
type: Component
persistent identity: component2243685
display id: component2243685
version: 1
top level: BBa_S03518
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0034
type: Component
persistent identity: component2243681
display id: component2243681
version: 1
top level: BBa_S03518
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243681
display id: annotation2243681
version: 1
top level: BBa_S03518
location: https://synbiohub.org/public/igem/BBa_S03518/annotation2243681/range2243681/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03518/annotation2243681/range2243681/1
type: Range
persistent identity: range2243681
display id: range2243681
version: 1
direction: direction/reverse
top level: BBa_S03518
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2243685
display id: annotation2243685
version: 1
top level: BBa_S03518
location: https://synbiohub.org/public/igem/BBa_S03518/annotation2243685/range2243685/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_S03518/annotation2243685/range2243685/1
type: Range
persistent identity: range2243685
display id: range2243685
version: 1
direction: direction/reverse
top level: BBa_S03518
start: 19
end: 703
orientation: inline

BBa_K117002
type: ComponentDefinition
persistent identity: BBa K117002
display id: BBa_K117002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LsrA promoter (indirectly activated by AI-2)
creator: Nguyen Xuan Hung
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The regulatory network for the uptake of Escherichia coli autoinducer 2 (AI-2) is comprised of a transporter complex, LsrABCD; its repressor, LsrR; and a cognate signal kinase, LsrK. This network is an integral part of the AI-2 quorum-sensing (QS) system. Once uptaken into the cell, AI-2 will be phosphorylated under the effect of LsrK. Without the presence of AI-2, LsrA promoter is inhibited by the repressor, LsrR. Phosphorylated AI-2 will prevent LsrR from inhibiting this promoter, hence activating it so that the downstream sequence can be expressed. To prevent the cell from self-inducing this promoter, we must suppress its AI-2 producing capability. It can be done by knocking out LuxS, one of the vital gene required for producing AI-2. By doing that, LsrA promoter can only be activated under the presence of AI-2 from other sources than the host cell which is LuxS mutant (say, by contact with normal cells). More information on AI-2 quorum-sensing (QS) system please refer to: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1952038
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RBS BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K117002_sequence/1

RBS BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1979372
display id: annotation1979372
version: 1
top level: BBa_K117002
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_K117002/annotation1979372/range1979372/1

https://synbiohub.org/public/igem/BBa_K117002/annotation1979372/range1979372/1
type: Range
persistent identity: range1979372
display id: range1979372
version: 1
direction: direction/forward
top level: BBa_K117002
start: 112
end: 123
orientation: inline

GFP2
type: ComponentDefinition
persistent identity: BBa I715020
display id: BBa_I715020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Carboxyl Half of GFP (aka GFP2)
creator: Will DeLoache
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A
mutable notes: A
mutable provenance: A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Coding Carboxyl half of GFP (aka GFP2)
sequence annotation: extra base to maintain reading frame
sequence annotation: Stop Codons (two in tandem)
sequence: https://synbiohub.org/public/igem/BBa_I715020_sequence/1

Coding Carboxyl half of GFP (aka GFP2)
type: SequenceAnnotation
persistent identity: annotation1935361
display id: annotation1935361
version: 1
top level: GFP2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935361/range1935361/1

https://synbiohub.org/public/igem/BBa_I715020/annotation1935361/range1935361/1
type: Range
persistent identity: range1935361
display id: range1935361
version: 1
direction: direction/forward
top level: GFP2
start: 1
end: 244
orientation: inline

extra base to maintain reading frame
type: SequenceAnnotation
persistent identity: annotation1935360
display id: annotation1935360
version: 1
top level: GFP2
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935360/range1935360/1

https://synbiohub.org/public/igem/BBa_I715020/annotation1935360/range1935360/1
type: Range
persistent identity: range1935360
display id: range1935360
version: 1
direction: direction/forward
top level: GFP2
start: 1
end: 1
orientation: inline

Stop Codons (two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935362
display id: annotation1935362
version: 1
top level: GFP2
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935362/range1935362/1

https://synbiohub.org/public/igem/BBa_I715020/annotation1935362/range1935362/1
type: Range
persistent identity: range1935362
display id: range1935362
version: 1
direction: direction/forward
top level: GFP2
start: 245
end: 250
orientation: inline

BBa_K081008
type: ComponentDefinition
persistent identity: BBa K081008
display id: BBa_K081008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxI protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: luxI protein synthesizes 3OC6HSL, which binds to LuxR. Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: luxI: RBS:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_C0061
sequence annotation: BBa_C0061
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081008_sequence/1

BBa_B0030
type: Component
persistent identity: component2243988
display id: component2243988
version: 1
top level: BBa_K081008
access: public
definition: BBa_B0030

BBa_C0061
type: Component
persistent identity: component2243991
display id: component2243991
version: 1
top level: BBa_K081008
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2243991
display id: annotation2243991
version: 1
top level: BBa_K081008
location: https://synbiohub.org/public/igem/BBa_K081008/annotation2243991/range2243991/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K081008/annotation2243991/range2243991/1
type: Range
persistent identity: range2243991
display id: range2243991
version: 1
direction: direction/forward
top level: BBa_K081008
start: 22
end: 639
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2243988
display id: annotation2243988
version: 1
top level: BBa_K081008
location: https://synbiohub.org/public/igem/BBa_K081008/annotation2243988/range2243988/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081008/annotation2243988/range2243988/1
type: Range
persistent identity: range2243988
display id: range2243988
version: 1
direction: direction/forward
top level: BBa_K081008
start: 1
end: 15
orientation: inline

Bla
type: ComponentDefinition
persistent identity: BBa I14018
display id: BBa_I14018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Bla)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: P(Bla), Medium Transcription
mutable provenance: Plasmid pUC19
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: P(Bla)
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I14018_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation1027016
display id: annotation1027016
version: 1
top level: Bla
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1027016/range1027016/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1027016/range1027016/1
type: Range
persistent identity: range1027016
display id: range1027016
version: 1
direction: direction/forward
top level: Bla
start: 25
end: 30
orientation: inline

P(Bla)
type: SequenceAnnotation
persistent identity: annotation1018027
display id: annotation1018027
version: 1
top level: Bla
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1018027/range1018027/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1018027/range1018027/1
type: Range
persistent identity: range1018027
display id: range1018027
version: 1
direction: direction/forward
top level: Bla
start: 1
end: 35
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1027020
display id: annotation1027020
version: 1
top level: Bla
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14018/annotation1027020/range1027020/1

https://synbiohub.org/public/igem/BBa_I14018/annotation1027020/range1027020/1
type: Range
persistent identity: range1027020
display id: range1027020
version: 1
direction: direction/forward
top level: Bla
start: 4
end: 9
orientation: inline

BBa_K091146
type: ComponentDefinition
persistent identity: BBa K091146
display id: BBa_K091146
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLas/Lux Hybrid Promoter
creator: Erin Feeney
created: 2008-07-16T11:00:00Z
modified: 2015-05-08T01:08:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 3067
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: K091146 is a modified version of the pLas' promoter (BBa_K091117) containing the addition of a luxR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of PAI-1 and LasR and repressed in the presence of AI-1 and LuxR.
mutable notes: The Lux box was placed adjacent to the LasR binding site with the aim of minimizing cross-talk in the presence of both PAI-1 and AI-1; since the LasR site should have a higher affinity, we hope that the binding of PAI-1 will competitively exclude the binding of AI-1.
mutable provenance: The LuxR binding site is from BBa_R0062 and the pLas' promoter is from the region upstream of the LasI gene in Pseudomonas aeruginosa.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LasR
sequence annotation: -35
sequence annotation: Transcriptional Start
sequence annotation: LuxR
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K091146_sequence/1

LasR
type: SequenceAnnotation
persistent identity: annotation1967354
display id: annotation1967354
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967354/range1967354/1
type: Range
persistent identity: range1967354
display id: range1967354
version: 1
direction: direction/forward
top level: BBa_K091146
start: 75
end: 93
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1967357
display id: annotation1967357
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967357/range1967357/1
type: Range
persistent identity: range1967357
display id: range1967357
version: 1
direction: direction/forward
top level: BBa_K091146
start: 86
end: 91
orientation: inline

Transcriptional Start
type: SequenceAnnotation
persistent identity: annotation1967358
display id: annotation1967358
version: 1
top level: BBa_K091146
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967358/range1967358/1
type: Range
persistent identity: range1967358
display id: range1967358
version: 1
direction: direction/forward
top level: BBa_K091146
start: 125
end: 125
orientation: inline

LuxR
type: SequenceAnnotation
persistent identity: annotation1967355
display id: annotation1967355
version: 1
top level: BBa_K091146
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967355/range1967355/1
type: Range
persistent identity: range1967355
display id: range1967355
version: 1
direction: direction/forward
top level: BBa_K091146
start: 94
end: 113
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1967356
display id: annotation1967356
version: 1
top level: BBa_K091146
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1

https://synbiohub.org/public/igem/BBa_K091146/annotation1967356/range1967356/1
type: Range
persistent identity: range1967356
display id: range1967356
version: 1
direction: direction/forward
top level: BBa_K091146
start: 113
end: 118
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0083
display id: BBa_R0083
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
mutable notes: The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown. The a at base 41 is mutated to t to avoid XbaI site. Speculation that this was a previous assembly site.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: A
sequence annotation: -35
sequence annotation: C1 OmpR
sequence annotation: linker
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0083_sequence/1

A
type: SequenceAnnotation
persistent identity: annotation306881
display id: annotation306881
version: 1
top level: OmpR
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1

https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1
type: Range
persistent identity: range306881
display id: range306881
version: 1
direction: direction/forward
top level: OmpR
start: 41
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301224
display id: annotation301224
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1
type: Range
persistent identity: range301224
display id: range301224
version: 1
direction: direction/forward
top level: OmpR
start: 45
end: 50
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301221
display id: annotation301221
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1
type: Range
persistent identity: range301221
display id: range301221
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation301223
display id: annotation301223
version: 1
top level: OmpR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1
type: Range
persistent identity: range301223
display id: range301223
version: 1
direction: direction/forward
top level: OmpR
start: 37
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301226
display id: annotation301226
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1

https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1
type: Range
persistent identity: range301226
display id: range301226
version: 1
direction: direction/forward
top level: OmpR
start: 68
end: 73
orientation: inline

cinR
type: ComponentDefinition
persistent identity: BBa R0078
display id: BBa_R0078
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (cinR and HSL regulated)
creator: Drew Endy
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: unidentified/uncharacterized CinR dependent
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_R0078_sequence/1

unidentified/uncharacterized CinR dependent
type: SequenceAnnotation
persistent identity: annotation318234
display id: annotation318234
version: 1
top level: cinR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318234/range318234/1
type: Range
persistent identity: range318234
display id: range318234
version: 1
direction: direction/forward
top level: cinR
start: 1
end: 220
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318244
display id: annotation318244
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318244/range318244/1
type: Range
persistent identity: range318244
display id: range318244
version: 1
direction: direction/forward
top level: cinR
start: 35
end: 74
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation318250
display id: annotation318250
version: 1
top level: cinR
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1

https://synbiohub.org/public/igem/BBa_R0078/annotation318250/range318250/1
type: Range
persistent identity: range318250
display id: range318250
version: 1
direction: direction/forward
top level: cinR
start: 91
end: 136
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: C3 OmpR
sequence annotation: -10
sequence annotation: C2 OmpR
sequence annotation: C1 OmpR
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
direction: direction/forward
top level: OmpR
start: 75
end: 80
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
direction: direction/forward
top level: OmpR
start: 54
end: 71
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
direction: direction/forward
top level: OmpR
start: 98
end: 103
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
direction: direction/forward
top level: OmpR
start: 34
end: 51
orientation: inline

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
direction: direction/forward
top level: OmpR
start: 13
end: 30
orientation: inline

cI TP901
type: ComponentDefinition
persistent identity: BBa C0075
display id: BBa_C0075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: TP901 cI repressor from phage TP901-1 (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Dimeric repressor from bacteriophage TP901-1 with dissociation constant of 2*10^-9. Sequence was translated with the E.Coli K12 codon usage table, and nucleotides 84-86 was changed from TTC to TTT to avoid the cut site GAATTC (81-86). Bronsted, L., Hammer, K., Johansen, A.H., 2003. Identification of operator sites of the CI repressor of the phage TP901-1: evolutionary link to the other phages. Virology 311, 144-156. cI sequence slightly different from part C0051. NCBI locus: NC_001416
mutable provenance: TP901-1
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: cI tp901
sequence: https://synbiohub.org/public/igem/BBa_C0075_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation306574
display id: annotation306574
version: 1
top level: cI TP901
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0075/annotation306574/range306574/1

https://synbiohub.org/public/igem/BBa_C0075/annotation306574/range306574/1
type: Range
persistent identity: range306574
display id: range306574
version: 1
direction: direction/forward
top level: cI TP901
start: 541
end: 573
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214007
display id: annotation2214007
version: 1
top level: cI TP901
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0075/annotation2214007/range2214007/1

https://synbiohub.org/public/igem/BBa_C0075/annotation2214007/range2214007/1
type: Range
persistent identity: range2214007
display id: range2214007
version: 1
direction: direction/forward
top level: cI TP901
start: 580
end: 604
orientation: inline

cI tp901
type: SequenceAnnotation
persistent identity: annotation302645
display id: annotation302645
version: 1
top level: cI TP901
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0075/annotation302645/range302645/1

https://synbiohub.org/public/igem/BBa_C0075/annotation302645/range302645/1
type: Range
persistent identity: range302645
display id: range302645
version: 1
direction: direction/forward
top level: cI TP901
start: 1
end: 540
orientation: inline

cinr
type: ComponentDefinition
persistent identity: BBa C0077
display id: BBa_C0077
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cinR activator from Rhizobium leguminosarum (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tag -> taATAA
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: cinR
sequence: https://synbiohub.org/public/igem/BBa_C0077_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214000
display id: annotation2214000
version: 1
top level: cinr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1

https://synbiohub.org/public/igem/BBa_C0077/annotation2214000/range2214000/1
type: Range
persistent identity: range2214000
display id: range2214000
version: 1
direction: direction/forward
top level: cinr
start: 763
end: 787
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306600
display id: annotation306600
version: 1
top level: cinr
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1

https://synbiohub.org/public/igem/BBa_C0077/annotation306600/range306600/1
type: Range
persistent identity: range306600
display id: range306600
version: 1
direction: direction/forward
top level: cinr
start: 724
end: 756
orientation: inline

cinR
type: SequenceAnnotation
persistent identity: annotation301112
display id: annotation301112
version: 1
top level: cinr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1

https://synbiohub.org/public/igem/BBa_C0077/annotation301112/range301112/1
type: Range
persistent identity: range301112
display id: range301112
version: 1
direction: direction/forward
top level: cinr
start: 1
end: 723
orientation: inline

BBa_E5504
type: ComponentDefinition
persistent identity: BBa E5504
display id: BBa_E5504
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0032.E0040
creator: jcbraff
created: 2005-05-16T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0040.B0032.E0040 (R0040.E5501)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_B0032
component: BBa_E0040
sequence annotation: BBa_R0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_E5504_sequence/1

BBa_R0040
type: Component
persistent identity: component1489160
display id: component1489160
version: 1
top level: BBa_E5504
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0032
type: Component
persistent identity: component1489171
display id: component1489171
version: 1
top level: BBa_E5504
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E0040
type: Component
persistent identity: component1489174
display id: component1489174
version: 1
top level: BBa_E5504
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1489160
display id: annotation1489160
version: 1
top level: BBa_E5504
location: https://synbiohub.org/public/igem/BBa_E5504/annotation1489160/range1489160/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_E5504/annotation1489160/range1489160/1
type: Range
persistent identity: range1489160
display id: range1489160
version: 1
direction: direction/forward
top level: BBa_E5504
start: 1
end: 54
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1489174
display id: annotation1489174
version: 1
top level: BBa_E5504
location: https://synbiohub.org/public/igem/BBa_E5504/annotation1489174/range1489174/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E5504/annotation1489174/range1489174/1
type: Range
persistent identity: range1489174
display id: range1489174
version: 1
direction: direction/forward
top level: BBa_E5504
start: 82
end: 801
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1489171
display id: annotation1489171
version: 1
top level: BBa_E5504
location: https://synbiohub.org/public/igem/BBa_E5504/annotation1489171/range1489171/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E5504/annotation1489171/range1489171/1
type: Range
persistent identity: range1489171
display id: range1489171
version: 1
direction: direction/forward
top level: BBa_E5504
start: 63
end: 75
orientation: inline

ARL2A0103
type: ComponentDefinition
persistent identity: BBa I732107
display id: BBa_I732107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0103
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732107_sequence/1

BBa_I13404
type: ComponentDefinition
persistent identity: BBa I13404
display id: BBa_I13404
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.GFP + PBad CFP
creator: jkm
created: 2005-04-26T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 2
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Part for screening plasmid test (to be combined with an upstream inverter library).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0034
component: BBa_E0040
component: BBa_B0012
component: BBa_E0020
component: BBa_I0500
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0020
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I13404_sequence/1

BBa_B0010
type: Component
persistent identity: component1480497
display id: component1480497
version: 1
top level: BBa_I13404
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1480517
display id: component1480517
version: 1
top level: BBa_I13404
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1480524
display id: component1480524
version: 1
top level: BBa_I13404
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1480492
display id: component1480492
version: 1
top level: BBa_I13404
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1480507
display id: component1480507
version: 1
top level: BBa_I13404
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1480534
display id: component1480534
version: 1
top level: BBa_I13404
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component1480519
display id: component1480519
version: 1
top level: BBa_I13404
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_I0500
type: Component
persistent identity: component1480512
display id: component1480512
version: 1
top level: BBa_I13404
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component1480490
display id: component1480490
version: 1
top level: BBa_I13404
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1480492
display id: annotation1480492
version: 1
top level: BBa_I13404
location: https://synbiohub.org/public/igem/BBa_I13404/annotation1480492/range1480492/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13404/annotation1480492/range1480492/1
type: Range
persistent identity: range1480492
display id: range1480492
version: 1
direction: direction/forward
top level: BBa_I13404
start: 19
end: 738
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1480490
display id: annotation1480490
version: 1
top level: BBa_I13404
location: https://synbiohub.org/public/igem/BBa_I13404/annotation1480490/range1480490/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13404/annotation1480490/range1480490/1
type: Range
persistent identity: range1480490
display id: range1480490
version: 1
direction: direction/forward
top level: BBa_I13404
start: 1
end: 12
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1480517
display id: annotation1480517
version: 1
top level: BBa_I13404
location: https://synbiohub.org/public/igem/BBa_I13404/annotation1480517/range1480517/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13404/annotation1480517/range1480517/1
type: Range
persistent identity: range1480517
display id: range1480517
version: 1
direction: direction/forward
top level: BBa_I13404
start: 2102
end: 2113
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1480507
display id: annotation1480507
version: 1
top level: BBa_I13404
location: https://synbiohub.org/public/igem/BBa_I13404/annotation1480507/range1480507/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13404/annotation1480507/range1480507/1
type: Range
persistent identity: range1480507
display id: range1480507
version: 1
direction: direction/forward
top level: BBa_I13404
start: 835
end: 875
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1480534
display id: annotation1480534
version: 1
top level: BBa_I13404
location: https://synbiohub.org/public/igem/BBa_I13404/annotation1480534/range1480534/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13404/annotation1480534/range1480534/1
type: Range
persistent identity: range1480534
display id: range1480534
version: 1
direction: direction/forward
top level: BBa_I13404
start: 2939
end: 2979
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1480524
display id: annotation1480524
version: 1
top level: BBa_I13404
location: https://synbiohub.org/public/igem/BBa_I13404/annotation1480524/range1480524/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13404/annotation1480524/range1480524/1
type: Range
persistent identity: range1480524
display id: range1480524
version: 1
direction: direction/forward
top level: BBa_I13404
start: 2851
end: 2930
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1480519
display id: annotation1480519
version: 1
top level: BBa_I13404
location: https://synbiohub.org/public/igem/BBa_I13404/annotation1480519/range1480519/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13404/annotation1480519/range1480519/1
type: Range
persistent identity: range1480519
display id: range1480519
version: 1
direction: direction/forward
top level: BBa_I13404
start: 2120
end: 2842
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1480512
display id: annotation1480512
version: 1
top level: BBa_I13404
location: https://synbiohub.org/public/igem/BBa_I13404/annotation1480512/range1480512/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13404/annotation1480512/range1480512/1
type: Range
persistent identity: range1480512
display id: range1480512
version: 1
direction: direction/forward
top level: BBa_I13404
start: 884
end: 2093
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1480497
display id: annotation1480497
version: 1
top level: BBa_I13404
location: https://synbiohub.org/public/igem/BBa_I13404/annotation1480497/range1480497/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13404/annotation1480497/range1480497/1
type: Range
persistent identity: range1480497
display id: range1480497
version: 1
direction: direction/forward
top level: BBa_I13404
start: 747
end: 826
orientation: inline

cinI
type: ComponentDefinition
persistent identity: BBa C0076
display id: BBa_C0076
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: cinI codes for an autoinducer synthetase which utilizes SAM to make O3-C14:1-HSL. In complex with O3-C14:1-HSL, CinR (BBa_C0077) binds to the Cin promoter (BBa_R0077) and activates transcription.
mutable notes: The regulatory locus cinRI in Rhizobium leguminosarum conrols a network of quorum-sensing loci Lithgow, JK; Wilkinson, A; Hardman, A; Rodelas, B; Wisniewski-Dye, F; Williams, P; Downie, AJ MOL. MICROBIOLOGY 37(1): 81-97, 2000 Change log: original STOP: tga -> tAaTAA
mutable provenance: Rhizobium leguminosarum
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: CinI
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0076_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214001
display id: annotation2214001
version: 1
top level: cinI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1

https://synbiohub.org/public/igem/BBa_C0076/annotation2214001/range2214001/1
type: Range
persistent identity: range2214001
display id: range2214001
version: 1
direction: direction/forward
top level: cinI
start: 703
end: 727
orientation: inline

CinI
type: SequenceAnnotation
persistent identity: annotation301035
display id: annotation301035
version: 1
top level: cinI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1

https://synbiohub.org/public/igem/BBa_C0076/annotation301035/range301035/1
type: Range
persistent identity: range301035
display id: range301035
version: 1
direction: direction/forward
top level: cinI
start: 1
end: 663
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306586
display id: annotation306586
version: 1
top level: cinI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1

https://synbiohub.org/public/igem/BBa_C0076/annotation306586/range306586/1
type: Range
persistent identity: range306586
display id: range306586
version: 1
direction: direction/forward
top level: cinI
start: 664
end: 696
orientation: inline

BBa_J24676
type: ComponentDefinition
persistent identity: BBa J24676
display id: BBa_J24676
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD to tetR coding region, lacI coding region, and mCherry reporter
creator: Annie Gao
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1017
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Links the pBAD promoter, which is induced by arabinose and inhibited by araC, to a tetR coding region with an RBS, a LacI coding region with an RBS, and an mCherry reporter. Activation of this pathway results in tetR production, lacI production, and mCherry production.
mutable notes: Strength of RBS may affect the strength of output.
mutable provenance: Composite of other iGEM parts
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J06702
component: BBa_S03518
component: BBa_I0500
component: BBa_S03520
sequence annotation: BBa_S03520
sequence annotation: BBa_S03518
sequence annotation: BBa_J06702
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_J24676_sequence/1

BBa_J06702
type: Component
persistent identity: component2274582
display id: component2274582
version: 1
top level: BBa_J24676
access: public
definition: mCherry

BBa_S03518
type: Component
persistent identity: component2274563
display id: component2274563
version: 1
top level: BBa_J24676
access: public
definition: BBa_S03518

BBa_I0500
type: Component
persistent identity: component2274556
display id: component2274556
version: 1
top level: BBa_J24676
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_S03520
type: Component
persistent identity: component2274570
display id: component2274570
version: 1
top level: BBa_J24676
access: public
definition: https://synbiohub.org/public/igem/BBa_S03520/1

BBa_S03520
type: SequenceAnnotation
persistent identity: annotation2274570
display id: annotation2274570
version: 1
top level: BBa_J24676
location: https://synbiohub.org/public/igem/BBa_J24676/annotation2274570/range2274570/1
component: BBa_S03520

https://synbiohub.org/public/igem/BBa_J24676/annotation2274570/range2274570/1
type: Range
persistent identity: range2274570
display id: range2274570
version: 1
direction: direction/reverse
top level: BBa_J24676
start: 1930
end: 3100
orientation: inline

BBa_S03518
type: SequenceAnnotation
persistent identity: annotation2274563
display id: annotation2274563
version: 1
top level: BBa_J24676
location: https://synbiohub.org/public/igem/BBa_J24676/annotation2274563/range2274563/1
component: BBa_S03518

https://synbiohub.org/public/igem/BBa_J24676/annotation2274563/range2274563/1
type: Range
persistent identity: range2274563
display id: range2274563
version: 1
direction: direction/reverse
top level: BBa_J24676
start: 1219
end: 1921
orientation: inline

BBa_J06702
type: SequenceAnnotation
persistent identity: annotation2274582
display id: annotation2274582
version: 1
top level: BBa_J24676
location: https://synbiohub.org/public/igem/BBa_J24676/annotation2274582/range2274582/1
component: BBa_J06702

https://synbiohub.org/public/igem/BBa_J24676/annotation2274582/range2274582/1
type: Range
persistent identity: range2274582
display id: range2274582
version: 1
direction: direction/reverse
top level: BBa_J24676
start: 3109
end: 3977
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation2274556
display id: annotation2274556
version: 1
top level: BBa_J24676
location: https://synbiohub.org/public/igem/BBa_J24676/annotation2274556/range2274556/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_J24676/annotation2274556/range2274556/1
type: Range
persistent identity: range2274556
display id: range2274556
version: 1
direction: direction/reverse
top level: BBa_J24676
start: 1
end: 1210
orientation: inline

BBa_S03650
type: ComponentDefinition
persistent identity: BBa S03650
display id: BBa_S03650
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0100:B0015
creator: Heather Keller
created: 2007-03-05T12:00:00Z
modified: 2015-05-08T01:14:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 571
owning group id: 10
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0010
component: BBa_B0101
component: BBa_B0012
sequence annotation: BBa_B0101
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S03650_sequence/1

BBa_B0010
type: Component
persistent identity: component1920489
display id: component1920489
version: 1
top level: BBa_S03650
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0101
type: Component
persistent identity: component1920488
display id: component1920488
version: 1
top level: BBa_S03650
access: public
definition: https://synbiohub.org/public/igem/BBa_B0101/1

BBa_B0012
type: Component
persistent identity: component1920491
display id: component1920491
version: 1
top level: BBa_S03650
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1920488
display id: annotation1920488
version: 1
top level: BBa_S03650
location: https://synbiohub.org/public/igem/BBa_S03650/annotation1920488/range1920488/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_S03650/annotation1920488/range1920488/1
type: Range
persistent identity: range1920488
display id: range1920488
version: 1
direction: direction/reverse
top level: BBa_S03650
start: 1
end: 58
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1920491
display id: annotation1920491
version: 1
top level: BBa_S03650
location: https://synbiohub.org/public/igem/BBa_S03650/annotation1920491/range1920491/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03650/annotation1920491/range1920491/1
type: Range
persistent identity: range1920491
display id: range1920491
version: 1
direction: direction/forward
top level: BBa_S03650
start: 155
end: 195
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1920489
display id: annotation1920489
version: 1
top level: BBa_S03650
location: https://synbiohub.org/public/igem/BBa_S03650/annotation1920489/range1920489/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03650/annotation1920489/range1920489/1
type: Range
persistent identity: range1920489
display id: range1920489
version: 1
direction: direction/forward
top level: BBa_S03650
start: 67
end: 146
orientation: inline

BBa_K145303
type: ComponentDefinition
persistent identity: BBa K145303
display id: BBa_K145303
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP generator controlled by key/lock
creator: Nathalie Busschaert
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: true
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device can generate GFP, but the production of GFP is normally switched off. Only when key is present, GFP will be produced. In that case fluorescence is detectable.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23032
component: BBa_J23109
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_J23109
sequence: https://synbiohub.org/public/igem/BBa_K145303_sequence/1

BBa_J23032
type: Component
persistent identity: component1983099
display id: component1983099
version: 1
top level: BBa_K145303
access: public
definition: https://synbiohub.org/public/igem/BBa_J23032/1

BBa_J23109
type: Component
persistent identity: component1983098
display id: component1983098
version: 1
top level: BBa_K145303
access: public
definition: https://synbiohub.org/public/igem/BBa_J23109/1

BBa_E0040
type: Component
persistent identity: component1983101
display id: component1983101
version: 1
top level: BBa_K145303
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1983104
display id: component1983104
version: 1
top level: BBa_K145303
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1983102
display id: component1983102
version: 1
top level: BBa_K145303
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1983104
display id: annotation1983104
version: 1
top level: BBa_K145303
location: https://synbiohub.org/public/igem/BBa_K145303/annotation1983104/range1983104/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K145303/annotation1983104/range1983104/1
type: Range
persistent identity: range1983104
display id: range1983104
version: 1
direction: direction/forward
top level: BBa_K145303
start: 909
end: 949
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation1983099
display id: annotation1983099
version: 1
top level: BBa_K145303
location: https://synbiohub.org/public/igem/BBa_K145303/annotation1983099/range1983099/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K145303/annotation1983099/range1983099/1
type: Range
persistent identity: range1983099
display id: range1983099
version: 1
direction: direction/reverse
top level: BBa_K145303
start: 44
end: 86
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1983101
display id: annotation1983101
version: 1
top level: BBa_K145303
location: https://synbiohub.org/public/igem/BBa_K145303/annotation1983101/range1983101/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K145303/annotation1983101/range1983101/1
type: Range
persistent identity: range1983101
display id: range1983101
version: 1
direction: direction/reverse
top level: BBa_K145303
start: 93
end: 812
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1983102
display id: annotation1983102
version: 1
top level: BBa_K145303
location: https://synbiohub.org/public/igem/BBa_K145303/annotation1983102/range1983102/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K145303/annotation1983102/range1983102/1
type: Range
persistent identity: range1983102
display id: range1983102
version: 1
direction: direction/forward
top level: BBa_K145303
start: 821
end: 900
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation1983098
display id: annotation1983098
version: 1
top level: BBa_K145303
location: https://synbiohub.org/public/igem/BBa_K145303/annotation1983098/range1983098/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K145303/annotation1983098/range1983098/1
type: Range
persistent identity: range1983098
display id: range1983098
version: 1
direction: direction/reverse
top level: BBa_K145303
start: 1
end: 35
orientation: inline

BBa_S0104
type: ComponentDefinition
persistent identity: BBa S0104
display id: BBa_S0104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0052
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0052
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0052
sequence: https://synbiohub.org/public/igem/BBa_S0104_sequence/1

BBa_C0052
type: Component
persistent identity: component939772
display id: component939772
version: 1
top level: BBa_S0104
access: public
definition: https://synbiohub.org/public/igem/BBa_C0052/1

BBa_B0034
type: Component
persistent identity: component939762
display id: component939762
version: 1
top level: BBa_S0104
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939762
display id: annotation939762
version: 1
top level: BBa_S0104
location: https://synbiohub.org/public/igem/BBa_S0104/annotation939762/range939762/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0104/annotation939762/range939762/1
type: Range
persistent identity: range939762
display id: range939762
version: 1
direction: direction/forward
top level: BBa_S0104
start: 1
end: 12
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation939772
display id: annotation939772
version: 1
top level: BBa_S0104
location: https://synbiohub.org/public/igem/BBa_S0104/annotation939772/range939772/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_S0104/annotation939772/range939772/1
type: Range
persistent identity: range939772
display id: range939772
version: 1
direction: direction/forward
top level: BBa_S0104
start: 19
end: 687
orientation: inline

BBa_K081017
type: ComponentDefinition
persistent identity: BBa K081017
display id: BBa_K081017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcyA protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: true
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: pcyA converts biliverdin IXalpha (BV) to phycocyanobilin (PCB). Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: pcyA:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_I15009
sequence annotation: BBa_I15009
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081017_sequence/1

BBa_B0030
type: Component
persistent identity: component2244002
display id: component2244002
version: 1
top level: BBa_K081017
access: public
definition: BBa_B0030

BBa_I15009
type: Component
persistent identity: component2244006
display id: component2244006
version: 1
top level: BBa_K081017
access: public
definition: BBa_I15009

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2244006
display id: annotation2244006
version: 1
top level: BBa_K081017
location: https://synbiohub.org/public/igem/BBa_K081017/annotation2244006/range2244006/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K081017/annotation2244006/range2244006/1
type: Range
persistent identity: range2244006
display id: range2244006
version: 1
direction: direction/reverse
top level: BBa_K081017
start: 22
end: 796
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2244002
display id: annotation2244002
version: 1
top level: BBa_K081017
location: https://synbiohub.org/public/igem/BBa_K081017/annotation2244002/range2244002/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081017/annotation2244002/range2244002/1
type: Range
persistent identity: range2244002
display id: range2244002
version: 1
direction: direction/forward
top level: BBa_K081017
start: 1
end: 15
orientation: inline

GFP1
type: ComponentDefinition
persistent identity: BBa I715019
display id: BBa_I715019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Amino Half of GFP (aka GFP1)
creator: Will DeLoache
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A
mutable notes: A
mutable provenance: A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Coding Amino Portion GFP (aka GFP1)
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_I715019_sequence/1

Coding Amino Portion GFP (aka GFP1)
type: SequenceAnnotation
persistent identity: annotation1935357
display id: annotation1935357
version: 1
top level: GFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715019/annotation1935357/range1935357/1

https://synbiohub.org/public/igem/BBa_I715019/annotation1935357/range1935357/1
type: Range
persistent identity: range1935357
display id: range1935357
version: 1
direction: direction/forward
top level: GFP1
start: 1
end: 471
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1935356
display id: annotation1935356
version: 1
top level: GFP1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I715019/annotation1935356/range1935356/1

https://synbiohub.org/public/igem/BBa_I715019/annotation1935356/range1935356/1
type: Range
persistent identity: range1935356
display id: range1935356
version: 1
direction: direction/forward
top level: GFP1
start: 1
end: 3
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0036
display id: BBa_E0036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0036_sequence/1

BBa_I718015
type: ComponentDefinition
persistent identity: BBa I718015
display id: BBa_I718015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ter-lox66-rbs-dapA (B. Subtilis)
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: downstream construct of the synthetic multicellular bacteria
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_I718016
component: BBa_B0012
component: BBa_I718005
component: BBa_B0030
sequence annotation: BBa_I718005
sequence annotation: BBa_I718016
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I718015_sequence/1

BBa_B0010
type: Component
persistent identity: component1956322
display id: component1956322
version: 1
top level: BBa_I718015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I718016
type: Component
persistent identity: component1956328
display id: component1956328
version: 1
top level: BBa_I718015
access: public
definition: https://synbiohub.org/public/igem/BBa_I718016/1

BBa_B0012
type: Component
persistent identity: component1956324
display id: component1956324
version: 1
top level: BBa_I718015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I718005
type: Component
persistent identity: component1956335
display id: component1956335
version: 1
top level: BBa_I718015
access: public
definition: https://synbiohub.org/public/igem/BBa_I718005/1

BBa_B0030
type: Component
persistent identity: component1956330
display id: component1956330
version: 1
top level: BBa_I718015
access: public
definition: BBa_B0030

BBa_I718005
type: SequenceAnnotation
persistent identity: annotation1956335
display id: annotation1956335
version: 1
top level: BBa_I718015
location: https://synbiohub.org/public/igem/BBa_I718015/annotation1956335/range1956335/1
component: BBa_I718005

https://synbiohub.org/public/igem/BBa_I718015/annotation1956335/range1956335/1
type: Range
persistent identity: range1956335
display id: range1956335
version: 1
direction: direction/reverse
top level: BBa_I718015
start: 201
end: 1076
orientation: inline

BBa_I718016
type: SequenceAnnotation
persistent identity: annotation1956328
display id: annotation1956328
version: 1
top level: BBa_I718015
location: https://synbiohub.org/public/igem/BBa_I718015/annotation1956328/range1956328/1
component: BBa_I718016

https://synbiohub.org/public/igem/BBa_I718015/annotation1956328/range1956328/1
type: Range
persistent identity: range1956328
display id: range1956328
version: 1
direction: direction/reverse
top level: BBa_I718015
start: 138
end: 171
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956322
display id: annotation1956322
version: 1
top level: BBa_I718015
location: https://synbiohub.org/public/igem/BBa_I718015/annotation1956322/range1956322/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I718015/annotation1956322/range1956322/1
type: Range
persistent identity: range1956322
display id: range1956322
version: 1
direction: direction/forward
top level: BBa_I718015
start: 1
end: 80
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956330
display id: annotation1956330
version: 1
top level: BBa_I718015
location: https://synbiohub.org/public/igem/BBa_I718015/annotation1956330/range1956330/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718015/annotation1956330/range1956330/1
type: Range
persistent identity: range1956330
display id: range1956330
version: 1
direction: direction/forward
top level: BBa_I718015
start: 180
end: 194
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956324
display id: annotation1956324
version: 1
top level: BBa_I718015
location: https://synbiohub.org/public/igem/BBa_I718015/annotation1956324/range1956324/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I718015/annotation1956324/range1956324/1
type: Range
persistent identity: range1956324
display id: range1956324
version: 1
direction: direction/forward
top level: BBa_I718015
start: 89
end: 129
orientation: inline

BBa_I732913
type: ComponentDefinition
persistent identity: BBa I732913
display id: BBa_I732913
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [aTC] -> RFP
creator: Ma Rui
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I732086
component: BBa_B0015
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_I732086
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I732913_sequence/1

BBa_I732086
type: Component
persistent identity: component2270977
display id: component2270977
version: 1
top level: BBa_I732913
access: public
definition: https://synbiohub.org/public/igem/BBa_I732086/1

BBa_B0015
type: Component
persistent identity: component2270989
display id: component2270989
version: 1
top level: BBa_I732913
access: public
definition: BBa_B0015

BBa_B0034
type: Component
persistent identity: component2270979
display id: component2270979
version: 1
top level: BBa_I732913
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component2270982
display id: component2270982
version: 1
top level: BBa_I732913
access: public
definition: mRFP1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2270982
display id: annotation2270982
version: 1
top level: BBa_I732913
location: https://synbiohub.org/public/igem/BBa_I732913/annotation2270982/range2270982/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I732913/annotation2270982/range2270982/1
type: Range
persistent identity: range2270982
display id: range2270982
version: 1
direction: direction/reverse
top level: BBa_I732913
start: 915
end: 1620
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2270979
display id: annotation2270979
version: 1
top level: BBa_I732913
location: https://synbiohub.org/public/igem/BBa_I732913/annotation2270979/range2270979/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732913/annotation2270979/range2270979/1
type: Range
persistent identity: range2270979
display id: range2270979
version: 1
direction: direction/reverse
top level: BBa_I732913
start: 897
end: 908
orientation: inline

BBa_I732086
type: SequenceAnnotation
persistent identity: annotation2270977
display id: annotation2270977
version: 1
top level: BBa_I732913
location: https://synbiohub.org/public/igem/BBa_I732913/annotation2270977/range2270977/1
component: BBa_I732086

https://synbiohub.org/public/igem/BBa_I732913/annotation2270977/range2270977/1
type: Range
persistent identity: range2270977
display id: range2270977
version: 1
direction: direction/reverse
top level: BBa_I732913
start: 1
end: 888
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2270989
display id: annotation2270989
version: 1
top level: BBa_I732913
location: https://synbiohub.org/public/igem/BBa_I732913/annotation2270989/range2270989/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I732913/annotation2270989/range2270989/1
type: Range
persistent identity: range2270989
display id: range2270989
version: 1
direction: direction/reverse
top level: BBa_I732913
start: 1629
end: 1757
orientation: inline

BBa_I13517
type: ComponentDefinition
persistent identity: BBa I13517
display id: BBa_I13517
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-06-05T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0032
component: BBa_E1010
component: BBa_I0500
sequence annotation: BBa_E1010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I13517_sequence/1

BBa_B0032
type: Component
persistent identity: component1505513
display id: component1505513
version: 1
top level: BBa_I13517
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E1010
type: Component
persistent identity: component1505521
display id: component1505521
version: 1
top level: BBa_I13517
access: public
definition: mRFP1

BBa_I0500
type: Component
persistent identity: component1505505
display id: component1505505
version: 1
top level: BBa_I13517
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505521
display id: annotation1505521
version: 1
top level: BBa_I13517
location: https://synbiohub.org/public/igem/BBa_I13517/annotation1505521/range1505521/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13517/annotation1505521/range1505521/1
type: Range
persistent identity: range1505521
display id: range1505521
version: 1
direction: direction/forward
top level: BBa_I13517
start: 1238
end: 1918
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1505505
display id: annotation1505505
version: 1
top level: BBa_I13517
location: https://synbiohub.org/public/igem/BBa_I13517/annotation1505505/range1505505/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13517/annotation1505505/range1505505/1
type: Range
persistent identity: range1505505
display id: range1505505
version: 1
direction: direction/forward
top level: BBa_I13517
start: 1
end: 1210
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1505513
display id: annotation1505513
version: 1
top level: BBa_I13517
location: https://synbiohub.org/public/igem/BBa_I13517/annotation1505513/range1505513/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I13517/annotation1505513/range1505513/1
type: Range
persistent identity: range1505513
display id: range1505513
version: 1
direction: direction/forward
top level: BBa_I13517
start: 1219
end: 1231
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member
creator: John Anderson
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Later
mutable notes: N/A
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

P22 attB
type: ComponentDefinition
persistent identity: BBa I11032
display id: BBa_I11032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P22 ''attB'', reverse complement
creator: mschomp
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reverse Complement of P22 attB site (B'-O-B)
mutable provenance: gi|49175990:262118-262144 rev comp
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11032_sequence/1

BBa_I13535
type: ComponentDefinition
persistent identity: BBa I13535
display id: BBa_I13535
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Test construct for screening plasmid
creator: Josh Michener
created: 2005-07-28T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Term.Pbad
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I13453
component: BBa_B0015
sequence annotation: BBa_I13453
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_I13535_sequence/1

BBa_I13453
type: Component
persistent identity: component2219239
display id: component2219239
version: 1
top level: BBa_I13535
access: public
definition: BBa_I13453

BBa_B0015
type: Component
persistent identity: component2219238
display id: component2219238
version: 1
top level: BBa_I13535
access: public
definition: BBa_B0015

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2219239
display id: annotation2219239
version: 1
top level: BBa_I13535
location: https://synbiohub.org/public/igem/BBa_I13535/annotation2219239/range2219239/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13535/annotation2219239/range2219239/1
type: Range
persistent identity: range2219239
display id: range2219239
version: 1
direction: direction/reverse
top level: BBa_I13535
start: 138
end: 267
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2219238
display id: annotation2219238
version: 1
top level: BBa_I13535
location: https://synbiohub.org/public/igem/BBa_I13535/annotation2219238/range2219238/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I13535/annotation2219238/range2219238/1
type: Range
persistent identity: range2219238
display id: range2219238
version: 1
direction: direction/reverse
top level: BBa_I13535
start: 1
end: 129
orientation: inline

BBa_K145215
type: ComponentDefinition
persistent identity: BBa K145215
display id: BBa_K145215
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: FILTER Key (TetR promoter + key)
creator: Jonas Demeulemeester
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: FILTER This device was designed to filter out noise in an input signal; in our case OmpF PoPs. If there is a short increase in transcription from this promoter, a small amount of J23066 RiboKey is produced. This is then used to unlock (J23078) the short lived UmuD-tagged T7 RNA polymerase. This results in an increase of active T7 protein. The final part of this filter is an AND gate which is used in the components further on in the system. This AND gate consists of a T7 RNA polymerase promoter with another RiboLock (J23078 again). The AND gate will only function if the duration of the noise is sufficiently long and intense (= a signal) in order to have both the short-lived RiboKey AND T7 polymerase available at the same time and thus to produce the component.
mutable notes: We did a lot of modeling on this device. The most efficient Berkeley 2006 key and lock have been used. The RiboKy might be required in higher copy number although modeling doesn't predict this.
mutable provenance: other parts
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_J23009
component: BBa_R0040
component: BBa_B0012
component: BBa_J23008
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J23008
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_J23009
sequence: https://synbiohub.org/public/igem/BBa_K145215_sequence/1

BBa_J23009
type: Component
persistent identity: component1974068
display id: component1974068
version: 1
top level: BBa_K145215
access: public
definition: https://synbiohub.org/public/igem/BBa_J23009/1

BBa_R0040
type: Component
persistent identity: component1974063
display id: component1974063
version: 1
top level: BBa_K145215
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component1974071
display id: component1974071
version: 1
top level: BBa_K145215
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J23008
type: Component
persistent identity: component1974067
display id: component1974067
version: 1
top level: BBa_K145215
access: public
definition: https://synbiohub.org/public/igem/BBa_J23008/1

BBa_B0010
type: Component
persistent identity: component1974069
display id: component1974069
version: 1
top level: BBa_K145215
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1974071
display id: annotation1974071
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974071/range1974071/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K145215/annotation1974071/range1974071/1
type: Range
persistent identity: range1974071
display id: range1974071
version: 1
direction: direction/forward
top level: BBa_K145215
start: 358
end: 398
orientation: inline

BBa_J23008
type: SequenceAnnotation
persistent identity: annotation1974067
display id: annotation1974067
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974067/range1974067/1
component: BBa_J23008

https://synbiohub.org/public/igem/BBa_K145215/annotation1974067/range1974067/1
type: Range
persistent identity: range1974067
display id: range1974067
version: 1
direction: direction/reverse
top level: BBa_K145215
start: 63
end: 156
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1974069
display id: annotation1974069
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974069/range1974069/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K145215/annotation1974069/range1974069/1
type: Range
persistent identity: range1974069
display id: range1974069
version: 1
direction: direction/forward
top level: BBa_K145215
start: 270
end: 349
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1974063
display id: annotation1974063
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974063/range1974063/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K145215/annotation1974063/range1974063/1
type: Range
persistent identity: range1974063
display id: range1974063
version: 1
direction: direction/forward
top level: BBa_K145215
start: 1
end: 54
orientation: inline

BBa_J23009
type: SequenceAnnotation
persistent identity: annotation1974068
display id: annotation1974068
version: 1
top level: BBa_K145215
location: https://synbiohub.org/public/igem/BBa_K145215/annotation1974068/range1974068/1
component: BBa_J23009

https://synbiohub.org/public/igem/BBa_K145215/annotation1974068/range1974068/1
type: Range
persistent identity: range1974068
display id: range1974068
version: 1
direction: direction/reverse
top level: BBa_K145215
start: 165
end: 261
orientation: inline

pLambda+RF
type: ComponentDefinition
persistent identity: BBa I763007
display id: BBa_I763007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter lambda (cI regulated) with RFP reporter
creator: Alice Pasini
created: 2007-07-29T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter lambda - cI regulated - (R0051) with RFP (E1010) as a reporter. It can be used as a detector of the glucose metabolism.
mutable notes: Reporter
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0051
component: BBa_I13507
sequence annotation: BBa_R0051
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_I763007_sequence/1

BBa_R0051
type: Component
persistent identity: component2252869
display id: component2252869
version: 1
top level: pLambda+RF
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_I13507
type: Component
persistent identity: component2252884
display id: component2252884
version: 1
top level: pLambda+RF
access: public
definition: https://synbiohub.org/public/igem/BBa_I13507/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2252869
display id: annotation2252869
version: 1
top level: pLambda+RF
location: https://synbiohub.org/public/igem/BBa_I763007/annotation2252869/range2252869/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I763007/annotation2252869/range2252869/1
type: Range
persistent identity: range2252869
display id: range2252869
version: 1
direction: direction/forward
top level: pLambda+RF
start: 1
end: 49
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2252884
display id: annotation2252884
version: 1
top level: pLambda+RF
location: https://synbiohub.org/public/igem/BBa_I763007/annotation2252884/range2252884/1
component: BBa_I13507

https://synbiohub.org/public/igem/BBa_I763007/annotation2252884/range2252884/1
type: Range
persistent identity: range2252884
display id: range2252884
version: 1
direction: direction/reverse
top level: pLambda+RF
start: 58
end: 918
orientation: inline

R6K
type: ComponentDefinition
persistent identity: BBa J61001
display id: BBa_J61001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [R6K] Origin of replication
creator: John Anderson
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T02:02:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R6K origin of replication requires a pir+ or pir116 strain for replication in the absence of a second origin. In most E. coli strains, this is a silent feature. {pSB1A2-Bca1011}
mutable notes: N/A
mutable provenance: PCR ca1011F/R on pG80ko (445 bp, EcoRI/SpeI) Sub into pSB1A2-I13522 (EcoRI/SpeI) Product is Bca1011 ---- ca1011F Forward Biobricking of R6K GGACTgaattcgcggccgcttctagagtgattcgcacgggcccatg ca1011R Reverse biobricking of R6K ccgctactagtaGCAGTTCAACCTGTTGATAG
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: R6K
sequence: https://synbiohub.org/public/igem/BBa_J61001_sequence/1

R6K
type: SequenceAnnotation
persistent identity: annotation1892271
display id: annotation1892271
version: 1
top level: R6K
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1

https://synbiohub.org/public/igem/BBa_J61001/annotation1892271/range1892271/1
type: Range
persistent identity: range1892271
display id: range1892271
version: 1
direction: direction/forward
top level: R6K
start: 27
end: 406
orientation: inline

BBa_I20270
type: ComponentDefinition
persistent identity: BBa I20270
display id: BBa_I20270
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter MeasKit (J23151)
creator: Jason Kelly
created: 2008-02-06T12:00:00Z
modified: 2015-08-31T04:07:39Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: J23108 inserted in the Promoter MeasKit
mutable notes: .
mutable provenance: .
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_B0032
component: BBa_J23151
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_J23151
sequence annotation: BBa_B0032
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I20270_sequence/1

BBa_B0010
type: Component
persistent identity: component1961205
display id: component1961205
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1961201
display id: component1961201
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_J23151
type: Component
persistent identity: component1961199
display id: component1961199
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_J23151/1

BBa_E0040
type: Component
persistent identity: component1961204
display id: component1961204
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1961207
display id: component1961207
version: 1
top level: BBa_I20270
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J23151
type: SequenceAnnotation
persistent identity: annotation1961199
display id: annotation1961199
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961199/range1961199/1
component: BBa_J23151

https://synbiohub.org/public/igem/BBa_I20270/annotation1961199/range1961199/1
type: Range
persistent identity: range1961199
display id: range1961199
version: 1
direction: direction/reverse
top level: BBa_I20270
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1961201
display id: annotation1961201
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961201/range1961201/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I20270/annotation1961201/range1961201/1
type: Range
persistent identity: range1961201
display id: range1961201
version: 1
direction: direction/forward
top level: BBa_I20270
start: 44
end: 56
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1961207
display id: annotation1961207
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961207/range1961207/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I20270/annotation1961207/range1961207/1
type: Range
persistent identity: range1961207
display id: range1961207
version: 1
direction: direction/forward
top level: BBa_I20270
start: 879
end: 919
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1961204
display id: annotation1961204
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961204/range1961204/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I20270/annotation1961204/range1961204/1
type: Range
persistent identity: range1961204
display id: range1961204
version: 1
direction: direction/reverse
top level: BBa_I20270
start: 63
end: 782
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1961205
display id: annotation1961205
version: 1
top level: BBa_I20270
location: https://synbiohub.org/public/igem/BBa_I20270/annotation1961205/range1961205/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I20270/annotation1961205/range1961205/1
type: Range
persistent identity: range1961205
display id: range1961205
version: 1
direction: direction/forward
top level: BBa_I20270
start: 791
end: 870
orientation: inline

BBa_K081019
type: ComponentDefinition
persistent identity: BBa K081019
display id: BBa_K081019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxR protein generator under Plambda regulation
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device produces luxR constitutively under the strong promoter Plambda, in absence of cI repressor. Plambda can be turned off by cI repressor and luxR production cannot continue. Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: Plambda: RBS: luxR: T:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_C0062
component: BBa_R0051
component: BBa_B1006
sequence annotation: BBa_R0051
sequence annotation: BBa_B0030
sequence annotation: BBa_B1006
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_K081019_sequence/1

BBa_B0030
type: Component
persistent identity: component2249293
display id: component2249293
version: 1
top level: BBa_K081019
access: public
definition: BBa_B0030

BBa_C0062
type: Component
persistent identity: component2249297
display id: component2249297
version: 1
top level: BBa_K081019
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_R0051
type: Component
persistent identity: component2249288
display id: component2249288
version: 1
top level: BBa_K081019
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B1006
type: Component
persistent identity: component2249305
display id: component2249305
version: 1
top level: BBa_K081019
access: public
definition: BBa_B1006

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2249288
display id: annotation2249288
version: 1
top level: BBa_K081019
location: https://synbiohub.org/public/igem/BBa_K081019/annotation2249288/range2249288/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K081019/annotation2249288/range2249288/1
type: Range
persistent identity: range2249288
display id: range2249288
version: 1
direction: direction/forward
top level: BBa_K081019
start: 1
end: 49
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249293
display id: annotation2249293
version: 1
top level: BBa_K081019
location: https://synbiohub.org/public/igem/BBa_K081019/annotation2249293/range2249293/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081019/annotation2249293/range2249293/1
type: Range
persistent identity: range2249293
display id: range2249293
version: 1
direction: direction/forward
top level: BBa_K081019
start: 58
end: 72
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2249305
display id: annotation2249305
version: 1
top level: BBa_K081019
location: https://synbiohub.org/public/igem/BBa_K081019/annotation2249305/range2249305/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081019/annotation2249305/range2249305/1
type: Range
persistent identity: range2249305
display id: range2249305
version: 1
direction: direction/reverse
top level: BBa_K081019
start: 868
end: 906
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2249297
display id: annotation2249297
version: 1
top level: BBa_K081019
location: https://synbiohub.org/public/igem/BBa_K081019/annotation2249297/range2249297/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K081019/annotation2249297/range2249297/1
type: Range
persistent identity: range2249297
display id: range2249297
version: 1
direction: direction/forward
top level: BBa_K081019
start: 79
end: 834
orientation: inline

BBa_I13515
type: ComponentDefinition
persistent identity: BBa I13515
display id: BBa_I13515
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 4
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The fourth screening plasmid. Same as the second, except without an insertion site. Sole purpose is to check whether the insertion site alone affects the performance. Ends should still be capped with I13450.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0034
component: BBa_E0040
component: BBa_E1010
component: BBa_I0500
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13515_sequence/1

BBa_B0034
type: Component
persistent identity: component1505441
display id: component1505441
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1505454
display id: component1505454
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1505456
display id: component1505456
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E1010
type: Component
persistent identity: component1505448
display id: component1505448
version: 1
top level: BBa_I13515
access: public
definition: mRFP1

BBa_I0500
type: Component
persistent identity: component1505436
display id: component1505436
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: Component
persistent identity: component1505471
display id: component1505471
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1505461
display id: component1505461
version: 1
top level: BBa_I13515
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505454
display id: annotation1505454
version: 1
top level: BBa_I13515
location: https://synbiohub.org/public/igem/BBa_I13515/annotation1505454/range1505454/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13515/annotation1505454/range1505454/1
type: Range
persistent identity: range1505454
display id: range1505454
version: 1
direction: direction/forward
top level: BBa_I13515
start: 1951
end: 1962
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505456
display id: annotation1505456
version: 1
top level: BBa_I13515
location: https://synbiohub.org/public/igem/BBa_I13515/annotation1505456/range1505456/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13515/annotation1505456/range1505456/1
type: Range
persistent identity: range1505456
display id: range1505456
version: 1
direction: direction/forward
top level: BBa_I13515
start: 1969
end: 2688
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505471
display id: annotation1505471
version: 1
top level: BBa_I13515
location: https://synbiohub.org/public/igem/BBa_I13515/annotation1505471/range1505471/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13515/annotation1505471/range1505471/1
type: Range
persistent identity: range1505471
display id: range1505471
version: 1
direction: direction/forward
top level: BBa_I13515
start: 2785
end: 2825
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505441
display id: annotation1505441
version: 1
top level: BBa_I13515
location: https://synbiohub.org/public/igem/BBa_I13515/annotation1505441/range1505441/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13515/annotation1505441/range1505441/1
type: Range
persistent identity: range1505441
display id: range1505441
version: 1
direction: direction/forward
top level: BBa_I13515
start: 1219
end: 1230
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505448
display id: annotation1505448
version: 1
top level: BBa_I13515
location: https://synbiohub.org/public/igem/BBa_I13515/annotation1505448/range1505448/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13515/annotation1505448/range1505448/1
type: Range
persistent identity: range1505448
display id: range1505448
version: 1
direction: direction/forward
top level: BBa_I13515
start: 1237
end: 1917
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1505436
display id: annotation1505436
version: 1
top level: BBa_I13515
location: https://synbiohub.org/public/igem/BBa_I13515/annotation1505436/range1505436/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13515/annotation1505436/range1505436/1
type: Range
persistent identity: range1505436
display id: range1505436
version: 1
direction: direction/forward
top level: BBa_I13515
start: 1
end: 1210
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505461
display id: annotation1505461
version: 1
top level: BBa_I13515
location: https://synbiohub.org/public/igem/BBa_I13515/annotation1505461/range1505461/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13515/annotation1505461/range1505461/1
type: Range
persistent identity: range1505461
display id: range1505461
version: 1
direction: direction/forward
top level: BBa_I13515
start: 2697
end: 2776
orientation: inline

BBa_J24675
type: ComponentDefinition
persistent identity: BBa J24675
display id: BBa_J24675
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS-> lacI and mCherry
creator: Annie Gao
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1017
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Links an lacI coding region with an RBS to mCherry reporter
mutable notes: No information
mutable provenance: Composite of other iGEM parts
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J06702
component: BBa_S03520
sequence annotation: BBa_S03520
sequence annotation: BBa_J06702
sequence: https://synbiohub.org/public/igem/BBa_J24675_sequence/1

BBa_J06702
type: Component
persistent identity: component2260366
display id: component2260366
version: 1
top level: BBa_J24675
access: public
definition: mCherry

BBa_S03520
type: Component
persistent identity: component2260354
display id: component2260354
version: 1
top level: BBa_J24675
access: public
definition: https://synbiohub.org/public/igem/BBa_S03520/1

BBa_S03520
type: SequenceAnnotation
persistent identity: annotation2260354
display id: annotation2260354
version: 1
top level: BBa_J24675
location: https://synbiohub.org/public/igem/BBa_J24675/annotation2260354/range2260354/1
component: BBa_S03520

https://synbiohub.org/public/igem/BBa_J24675/annotation2260354/range2260354/1
type: Range
persistent identity: range2260354
display id: range2260354
version: 1
direction: direction/reverse
top level: BBa_J24675
start: 1
end: 1171
orientation: inline

BBa_J06702
type: SequenceAnnotation
persistent identity: annotation2260366
display id: annotation2260366
version: 1
top level: BBa_J24675
location: https://synbiohub.org/public/igem/BBa_J24675/annotation2260366/range2260366/1
component: BBa_J06702

https://synbiohub.org/public/igem/BBa_J24675/annotation2260366/range2260366/1
type: Range
persistent identity: range2260366
display id: range2260366
version: 1
direction: direction/reverse
top level: BBa_J24675
start: 1180
end: 2048
orientation: inline

BBa_K081009
type: ComponentDefinition
persistent identity: BBa K081009
display id: BBa_K081009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasI protein generator (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: lasI: autoinducer synthetase for PAI from Pseudomonas aeruginosa. Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: lasI: RBS:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0078
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_C0078
sequence: https://synbiohub.org/public/igem/BBa_K081009_sequence/1

BBa_C0078
type: Component
persistent identity: component2244000
display id: component2244000
version: 1
top level: BBa_K081009
access: public
definition: lasI

BBa_B0030
type: Component
persistent identity: component2243995
display id: component2243995
version: 1
top level: BBa_K081009
access: public
definition: BBa_B0030

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2243995
display id: annotation2243995
version: 1
top level: BBa_K081009
location: https://synbiohub.org/public/igem/BBa_K081009/annotation2243995/range2243995/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081009/annotation2243995/range2243995/1
type: Range
persistent identity: range2243995
display id: range2243995
version: 1
direction: direction/forward
top level: BBa_K081009
start: 1
end: 15
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2244000
display id: annotation2244000
version: 1
top level: BBa_K081009
location: https://synbiohub.org/public/igem/BBa_K081009/annotation2244000/range2244000/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K081009/annotation2244000/range2244000/1
type: Range
persistent identity: range2244000
display id: range2244000
version: 1
direction: direction/reverse
top level: BBa_K081009
start: 22
end: 688
orientation: inline

BBa_K082034
type: ComponentDefinition
persistent identity: BBa K082034
display id: BBa_K082034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI regulated GFP generator
creator: Yanting Xue ,He Shen
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: none
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_K082003
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_K082003
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K082034_sequence/1

BBa_R0010
type: Component
persistent identity: component1992863
display id: component1992863
version: 1
top level: BBa_K082034
access: public
definition: LacI

BBa_K082003
type: Component
persistent identity: component1992872
display id: component1992872
version: 1
top level: BBa_K082034
access: public
definition: BBa_K082003

BBa_B0010
type: Component
persistent identity: component1992873
display id: component1992873
version: 1
top level: BBa_K082034
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1992871
display id: component1992871
version: 1
top level: BBa_K082034
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1992875
display id: component1992875
version: 1
top level: BBa_K082034
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1992873
display id: annotation1992873
version: 1
top level: BBa_K082034
location: https://synbiohub.org/public/igem/BBa_K082034/annotation1992873/range1992873/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K082034/annotation1992873/range1992873/1
type: Range
persistent identity: range1992873
display id: range1992873
version: 1
direction: direction/forward
top level: BBa_K082034
start: 991
end: 1070
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1992871
display id: annotation1992871
version: 1
top level: BBa_K082034
location: https://synbiohub.org/public/igem/BBa_K082034/annotation1992871/range1992871/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K082034/annotation1992871/range1992871/1
type: Range
persistent identity: range1992871
display id: range1992871
version: 1
direction: direction/reverse
top level: BBa_K082034
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1992863
display id: annotation1992863
version: 1
top level: BBa_K082034
location: https://synbiohub.org/public/igem/BBa_K082034/annotation1992863/range1992863/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K082034/annotation1992863/range1992863/1
type: Range
persistent identity: range1992863
display id: range1992863
version: 1
direction: direction/forward
top level: BBa_K082034
start: 1
end: 200
orientation: inline

BBa_K082003
type: SequenceAnnotation
persistent identity: annotation1992872
display id: annotation1992872
version: 1
top level: BBa_K082034
location: https://synbiohub.org/public/igem/BBa_K082034/annotation1992872/range1992872/1
component: BBa_K082003

https://synbiohub.org/public/igem/BBa_K082034/annotation1992872/range1992872/1
type: Range
persistent identity: range1992872
display id: range1992872
version: 1
direction: direction/reverse
top level: BBa_K082034
start: 227
end: 982
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1992875
display id: annotation1992875
version: 1
top level: BBa_K082034
location: https://synbiohub.org/public/igem/BBa_K082034/annotation1992875/range1992875/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K082034/annotation1992875/range1992875/1
type: Range
persistent identity: range1992875
display id: range1992875
version: 1
direction: direction/forward
top level: BBa_K082034
start: 1079
end: 1119
orientation: inline

BBa_J04771
type: ComponentDefinition
persistent identity: BBa J04771
display id: BBa_J04771
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BBa_J04700 (ForAptRibo) Riboswitch with BBa_J04671 (EYFP, RBS-, LVA+, B0015)
creator: Andrew Drysdale and Kristen DeCelle (also Oscar Hernandez and Matt Gemberling)
created: 2005-11-02T12:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _16_
m user id: 0
owner id: 435
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a (ForAptRibo) Riboswitch device that contains a EYFP reporter region with an LVA tag and a B0015 Terminator Sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0010
component: BBa_J04700
component: BBa_E0032
component: BBa_B0012
sequence annotation: BBa_E0032
sequence annotation: BBa_J04700
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J04771_sequence/1

BBa_B0010
type: Component
persistent identity: component1734981
display id: component1734981
version: 1
top level: BBa_J04771
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J04700
type: Component
persistent identity: component1734961
display id: component1734961
version: 1
top level: BBa_J04771
access: public
definition: https://synbiohub.org/public/igem/BBa_J04700/1

BBa_E0032
type: Component
persistent identity: component1734973
display id: component1734973
version: 1
top level: BBa_J04771
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0012
type: Component
persistent identity: component1734991
display id: component1734991
version: 1
top level: BBa_J04771
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1734973
display id: annotation1734973
version: 1
top level: BBa_J04771
location: https://synbiohub.org/public/igem/BBa_J04771/annotation1734973/range1734973/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_J04771/annotation1734973/range1734973/1
type: Range
persistent identity: range1734973
display id: range1734973
version: 1
direction: direction/forward
top level: BBa_J04771
start: 265
end: 1026
orientation: inline

BBa_J04700
type: SequenceAnnotation
persistent identity: annotation1734961
display id: annotation1734961
version: 1
top level: BBa_J04771
location: https://synbiohub.org/public/igem/BBa_J04771/annotation1734961/range1734961/1
component: BBa_J04700

https://synbiohub.org/public/igem/BBa_J04771/annotation1734961/range1734961/1
type: Range
persistent identity: range1734961
display id: range1734961
version: 1
direction: direction/forward
top level: BBa_J04771
start: 1
end: 258
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1734981
display id: annotation1734981
version: 1
top level: BBa_J04771
location: https://synbiohub.org/public/igem/BBa_J04771/annotation1734981/range1734981/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04771/annotation1734981/range1734981/1
type: Range
persistent identity: range1734981
display id: range1734981
version: 1
direction: direction/forward
top level: BBa_J04771
start: 1035
end: 1114
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1734991
display id: annotation1734991
version: 1
top level: BBa_J04771
location: https://synbiohub.org/public/igem/BBa_J04771/annotation1734991/range1734991/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04771/annotation1734991/range1734991/1
type: Range
persistent identity: range1734991
display id: range1734991
version: 1
direction: direction/forward
top level: BBa_J04771
start: 1123
end: 1163
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD strong
creator: Amelia Hardjasa
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type ( I13453 ) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
mutable notes: There were no special design considerations.
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraI2
sequence annotation: promoter
sequence annotation: AraI1
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
direction: direction/forward
top level: pBAD
start: 61
end: 78
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
direction: direction/forward
top level: pBAD
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
direction: direction/forward
top level: pBAD
start: 40
end: 57
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~>90)
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence annotation: B1006
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
direction: direction/forward
top level: BBa_B1006
start: 32
end: 39
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
direction: direction/forward
top level: BBa_B1006
start: 10
end: 31
orientation: inline

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
direction: direction/forward
top level: BBa_B1006
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
direction: direction/reverse
top level: BBa_B1006
start: 1
end: 9
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
creator: Drew Endy
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
direction: direction/forward
top level: mRFP1
start: 682
end: 706
orientation: inline

BBa_I13001
type: ComponentDefinition
persistent identity: BBa I13001
display id: BBa_I13001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS B0030 w/ YFP (-LVA) TT
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: B0030. E0030. B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_E0030
component: BBa_B0030
component: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13001_sequence/1

BBa_B0012
type: Component
persistent identity: component943446
display id: component943446
version: 1
top level: BBa_I13001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component943431
display id: component943431
version: 1
top level: BBa_I13001
access: public
definition: eyfp

BBa_B0030
type: Component
persistent identity: component943428
display id: component943428
version: 1
top level: BBa_I13001
access: public
definition: BBa_B0030

BBa_B0010
type: Component
persistent identity: component943436
display id: component943436
version: 1
top level: BBa_I13001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation943428
display id: annotation943428
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943428/range943428/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I13001/annotation943428/range943428/1
type: Range
persistent identity: range943428
display id: range943428
version: 1
direction: direction/forward
top level: BBa_I13001
start: 1
end: 15
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943436
display id: annotation943436
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943436/range943436/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13001/annotation943436/range943436/1
type: Range
persistent identity: range943436
display id: range943436
version: 1
direction: direction/forward
top level: BBa_I13001
start: 753
end: 832
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943431
display id: annotation943431
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943431/range943431/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13001/annotation943431/range943431/1
type: Range
persistent identity: range943431
display id: range943431
version: 1
direction: direction/forward
top level: BBa_I13001
start: 22
end: 744
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943446
display id: annotation943446
version: 1
top level: BBa_I13001
location: https://synbiohub.org/public/igem/BBa_I13001/annotation943446/range943446/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13001/annotation943446/range943446/1
type: Range
persistent identity: range943446
display id: range943446
version: 1
direction: direction/forward
top level: BBa_I13001
start: 841
end: 881
orientation: inline

BBa_K091100
type: ComponentDefinition
persistent identity: BBa K091100
display id: BBa_K091100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac_lux hybrid promoter
creator: Pallavi Penumetcha
created: 2008-05-28T11:00:00Z
modified: 2015-05-08T01:08:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _191_
m user id: 0
owner id: 2993
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This promoter is repressed by lacI and luxR with HSL. It contains a lux box and a lacI binding site.
mutable notes: Deleted the CAP binding site in R0010.
mutable provenance: R0010 modified with the insertion of a lux box in between -35 and -10.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lacI binding site
sequence annotation: lux box
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_K091100_sequence/1

lacI binding site
type: SequenceAnnotation
persistent identity: annotation1963336
display id: annotation1963336
version: 1
top level: BBa_K091100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963336/range1963336/1
type: Range
persistent identity: range1963336
display id: range1963336
version: 1
direction: direction/forward
top level: BBa_K091100
start: 40
end: 74
orientation: inline

lux box
type: SequenceAnnotation
persistent identity: annotation1963335
display id: annotation1963335
version: 1
top level: BBa_K091100
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963335/range1963335/1
type: Range
persistent identity: range1963335
display id: range1963335
version: 1
direction: direction/forward
top level: BBa_K091100
start: 16
end: 33
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1963334
display id: annotation1963334
version: 1
top level: BBa_K091100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963334/range1963334/1
type: Range
persistent identity: range1963334
display id: range1963334
version: 1
direction: direction/forward
top level: BBa_K091100
start: 34
end: 39
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1963333
display id: annotation1963333
version: 1
top level: BBa_K091100
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1

https://synbiohub.org/public/igem/BBa_K091100/annotation1963333/range1963333/1
type: Range
persistent identity: range1963333
display id: range1963333
version: 1
direction: direction/forward
top level: BBa_K091100
start: 10
end: 15
orientation: inline

lexA DBD
type: ComponentDefinition
persistent identity: BBa K165009
display id: BBa_K165009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LexA DNA-binding domain
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: -
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence: https://synbiohub.org/public/igem/BBa_K165009_sequence/1

BBa_K116638
type: ComponentDefinition
persistent identity: BBa K116638
display id: BBa_K116638
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac + RBS + LuxI + T
creator: Jesse Wu
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:33Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LuxI regulated by the pLac promoter. Used for expressing an adjustable amount of LuxI.
mutable notes: none
mutable provenance: ligation
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_R0010
component: BBa_K116625
sequence annotation: BBa_K116625
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_K116638_sequence/1

BBa_R0010
type: Component
persistent identity: component2254933
display id: component2254933
version: 1
top level: BBa_K116638
access: public
definition: LacI

BBa_K116625
type: Component
persistent identity: component2254955
display id: component2254955
version: 1
top level: BBa_K116638
access: public
definition: https://synbiohub.org/public/igem/BBa_K116625/1

BBa_K116625
type: SequenceAnnotation
persistent identity: annotation2254955
display id: annotation2254955
version: 1
top level: BBa_K116638
location: https://synbiohub.org/public/igem/BBa_K116638/annotation2254955/range2254955/1
component: BBa_K116625

https://synbiohub.org/public/igem/BBa_K116638/annotation2254955/range2254955/1
type: Range
persistent identity: range2254955
display id: range2254955
version: 1
direction: direction/reverse
top level: BBa_K116638
start: 209
end: 1007
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2254933
display id: annotation2254933
version: 1
top level: BBa_K116638
location: https://synbiohub.org/public/igem/BBa_K116638/annotation2254933/range2254933/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K116638/annotation2254933/range2254933/1
type: Range
persistent identity: range2254933
display id: range2254933
version: 1
direction: direction/forward
top level: BBa_K116638
start: 1
end: 200
orientation: inline

ARL2A0301
type: ComponentDefinition
persistent identity: BBa I732112
display id: BBa_I732112
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0301
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732112_sequence/1

BBa_J33202
type: ComponentDefinition
persistent identity: BBa J33202
display id: BBa_J33202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ
creator: Chris French
created: 2006-10-12T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This gene encodes the N-terminal 77 amino acid residues of LacZ. When expressed in an E. coli host carrying the lacZ-delta-M15 mutation, common in laboratory strains, it complements the delection resulting in the production of active LacZ, which can be detected by various means, including chromogenic substrates such as Xgal (5-bromo-4-chloro-3-indolyl-beta-D-galactoside) and ONPG (o-nitrophenyl galactoside).
mutable notes: Note that a stop codon was introduced to replace codon 78 of lacZ, resulting in production only of the N-terminal 77 amino acid residues of LacZ. This is sufficient to complement the lacZ-delta-M15 mutation commonly found in laboratory strains of E. col used for alpha-complementation.
mutable provenance: The DNA was derived by PCR from E. coli BL21, which possesses an intact lacZ gene. Primers were designed based on sequence from GenBank accession J01636, GI:146575. A stop codon was artificially introduced to replace codon 78. The sequence reported here is derived by sequencing of the Biobrick construct.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: lacZ'
sequence: https://synbiohub.org/public/igem/BBa_J33202_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1902818
display id: annotation1902818
version: 1
top level: BBa_J33202
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J33202/annotation1902818/range1902818/1

https://synbiohub.org/public/igem/BBa_J33202/annotation1902818/range1902818/1
type: Range
persistent identity: range1902818
display id: range1902818
version: 1
direction: direction/forward
top level: BBa_J33202
start: 1
end: 4
orientation: inline

lacZ'
type: SequenceAnnotation
persistent identity: annotation1902819
display id: annotation1902819
version: 1
top level: BBa_J33202
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J33202/annotation1902819/range1902819/1

https://synbiohub.org/public/igem/BBa_J33202/annotation1902819/range1902819/1
type: Range
persistent identity: range1902819
display id: range1902819
version: 1
direction: direction/forward
top level: BBa_J33202
start: 13
end: 243
orientation: inline

BBa_K081021
type: ComponentDefinition
persistent identity: BBa K081021
display id: BBa_K081021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pcyA protein generator - PoPS->pcyA
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: PoPS in -> pcyA out. Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: pcyA: T:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B1006
component: BBa_B0030
component: BBa_I15009
sequence annotation: BBa_I15009
sequence annotation: BBa_B0030
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081021_sequence/1

BBa_B1006
type: Component
persistent identity: component2246178
display id: component2246178
version: 1
top level: BBa_K081021
access: public
definition: BBa_B1006

BBa_B0030
type: Component
persistent identity: component2246169
display id: component2246169
version: 1
top level: BBa_K081021
access: public
definition: BBa_B0030

BBa_I15009
type: Component
persistent identity: component2246173
display id: component2246173
version: 1
top level: BBa_K081021
access: public
definition: BBa_I15009

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2246173
display id: annotation2246173
version: 1
top level: BBa_K081021
location: https://synbiohub.org/public/igem/BBa_K081021/annotation2246173/range2246173/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K081021/annotation2246173/range2246173/1
type: Range
persistent identity: range2246173
display id: range2246173
version: 1
direction: direction/reverse
top level: BBa_K081021
start: 22
end: 796
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246169
display id: annotation2246169
version: 1
top level: BBa_K081021
location: https://synbiohub.org/public/igem/BBa_K081021/annotation2246169/range2246169/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081021/annotation2246169/range2246169/1
type: Range
persistent identity: range2246169
display id: range2246169
version: 1
direction: direction/forward
top level: BBa_K081021
start: 1
end: 15
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2246178
display id: annotation2246178
version: 1
top level: BBa_K081021
location: https://synbiohub.org/public/igem/BBa_K081021/annotation2246178/range2246178/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081021/annotation2246178/range2246178/1
type: Range
persistent identity: range2246178
display id: range2246178
version: 1
direction: direction/reverse
top level: BBa_K081021
start: 805
end: 843
orientation: inline

BBa_I746907
type: ComponentDefinition
persistent identity: BBa I746907
display id: BBa_I746907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 promoter driving 6-his tagged P7 GFP
creator: Stefan Milde
created: 2008-09-30T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is used for purification of P7 GFP (for source and other information about this new GFP variant see its part description: I746917) via its 6-his tag. It is driven by a T7 promoter. We used E.coli BL21 (DE3) for expression of this part: addition of 1mM IPTG leads to expression of the T7 DNA polymerase which in turn drives expression of the tagged P7 GFP.
mutable notes: Expression requires T7 DNA polymerase.
mutable provenance: The part was created from intermediates via standard assembly.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I719005
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_I746920
sequence annotation: BBa_I746920
sequence annotation: BBa_I719005
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I746907_sequence/1

BBa_I719005
type: Component
persistent identity: component1978133
display id: component1978133
version: 1
top level: BBa_I746907
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_B0010
type: Component
persistent identity: component1978141
display id: component1978141
version: 1
top level: BBa_I746907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1978135
display id: component1978135
version: 1
top level: BBa_I746907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1978143
display id: component1978143
version: 1
top level: BBa_I746907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I746920
type: Component
persistent identity: component1978140
display id: component1978140
version: 1
top level: BBa_I746907
access: public
definition: https://synbiohub.org/public/igem/BBa_I746920/1

BBa_I746920
type: SequenceAnnotation
persistent identity: annotation1978140
display id: annotation1978140
version: 1
top level: BBa_I746907
location: https://synbiohub.org/public/igem/BBa_I746907/annotation1978140/range1978140/1
component: BBa_I746920

https://synbiohub.org/public/igem/BBa_I746907/annotation1978140/range1978140/1
type: Range
persistent identity: range1978140
display id: range1978140
version: 1
direction: direction/reverse
top level: BBa_I746907
start: 50
end: 787
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation1978133
display id: annotation1978133
version: 1
top level: BBa_I746907
location: https://synbiohub.org/public/igem/BBa_I746907/annotation1978133/range1978133/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_I746907/annotation1978133/range1978133/1
type: Range
persistent identity: range1978133
display id: range1978133
version: 1
direction: direction/reverse
top level: BBa_I746907
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1978135
display id: annotation1978135
version: 1
top level: BBa_I746907
location: https://synbiohub.org/public/igem/BBa_I746907/annotation1978135/range1978135/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746907/annotation1978135/range1978135/1
type: Range
persistent identity: range1978135
display id: range1978135
version: 1
direction: direction/reverse
top level: BBa_I746907
start: 32
end: 43
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1978143
display id: annotation1978143
version: 1
top level: BBa_I746907
location: https://synbiohub.org/public/igem/BBa_I746907/annotation1978143/range1978143/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746907/annotation1978143/range1978143/1
type: Range
persistent identity: range1978143
display id: range1978143
version: 1
direction: direction/forward
top level: BBa_I746907
start: 884
end: 924
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1978141
display id: annotation1978141
version: 1
top level: BBa_I746907
location: https://synbiohub.org/public/igem/BBa_I746907/annotation1978141/range1978141/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746907/annotation1978141/range1978141/1
type: Range
persistent identity: range1978141
display id: range1978141
version: 1
direction: direction/forward
top level: BBa_I746907
start: 796
end: 875
orientation: inline

BBa_S03506
type: ComponentDefinition
persistent identity: BBa S03506
display id: BBa_S03506
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:J32004
creator: Austen Heinz
created: 2006-07-16T11:00:00Z
modified: 2015-05-08T01:14:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_J32004
sequence annotation: BBa_J32004
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03506_sequence/1

BBa_B0034
type: Component
persistent identity: component1885772
display id: component1885772
version: 1
top level: BBa_S03506
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J32004
type: Component
persistent identity: component1885774
display id: component1885774
version: 1
top level: BBa_S03506
access: public
definition: https://synbiohub.org/public/igem/BBa_J32004/1

BBa_J32004
type: SequenceAnnotation
persistent identity: annotation1885774
display id: annotation1885774
version: 1
top level: BBa_S03506
location: https://synbiohub.org/public/igem/BBa_S03506/annotation1885774/range1885774/1
component: BBa_J32004

https://synbiohub.org/public/igem/BBa_S03506/annotation1885774/range1885774/1
type: Range
persistent identity: range1885774
display id: range1885774
version: 1
direction: direction/reverse
top level: BBa_S03506
start: 19
end: 237
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1885772
display id: annotation1885772
version: 1
top level: BBa_S03506
location: https://synbiohub.org/public/igem/BBa_S03506/annotation1885772/range1885772/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03506/annotation1885772/range1885772/1
type: Range
persistent identity: range1885772
display id: range1885772
version: 1
direction: direction/reverse
top level: BBa_S03506
start: 1
end: 12
orientation: inline

BBa_K105022
type: ComponentDefinition
persistent identity: BBa K105022
display id: BBa_K105022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lex A - operator
creator: Katja Karstens
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This BioBrick is the lex A recognition site. It can be used for the construction of promoters which should be regulated by activators or repressors containing a lex A DNA binding domain ( BBa_K105005).
mutable notes: This BioBrick contains the consensus sequence plus 5 spacer nucleotides on each side. The spacer is directly followed by the restriction site without a base in between. So when you construct multiple operator sites from this basic part you will have 16 bp between two consensus sequences.
mutable provenance: from oligos
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_K105022_sequence/1

consensus sequence
type: SequenceAnnotation
persistent identity: annotation1989120
display id: annotation1989120
version: 1
top level: BBa_K105022
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K105022/annotation1989120/range1989120/1

https://synbiohub.org/public/igem/BBa_K105022/annotation1989120/range1989120/1
type: Range
persistent identity: range1989120
display id: range1989120
version: 1
direction: direction/forward
top level: BBa_K105022
start: 6
end: 26
orientation: inline

BBa_K118015
type: ComponentDefinition
persistent identity: BBa K118015
display id: BBa_K118015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glgC coding sequence encoding ADP-glucose pyrophosphorylase
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' from ''Escherichia coli'' JM109. It encodes ADP-glucose pyrophosphorylase, which catalyses Glucose 1-phosphate + ATP -> ADP-glucose + PPi, a step in the production of glycogen from glucose.
mutable notes: The genomic DNA sequence contains to EcoRI sites, both of which have been mutated out using the mutagenesis by blunt-ended ligation (MABEL) technique
mutable provenance: ''Escherichia coli'' JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EcoRI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118015_sequence/1

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978556
display id: annotation1978556
version: 1
top level: BBa_K118015
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118015/annotation1978556/range1978556/1

https://synbiohub.org/public/igem/BBa_K118015/annotation1978556/range1978556/1
type: Range
persistent identity: range1978556
display id: range1978556
version: 1
direction: direction/forward
top level: BBa_K118015
start: 1068
end: 1073
orientation: inline

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978555
display id: annotation1978555
version: 1
top level: BBa_K118015
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118015/annotation1978555/range1978555/1

https://synbiohub.org/public/igem/BBa_K118015/annotation1978555/range1978555/1
type: Range
persistent identity: range1978555
display id: range1978555
version: 1
direction: direction/forward
top level: BBa_K118015
start: 571
end: 576
orientation: inline

BBa_S03575
type: ComponentDefinition
persistent identity: BBa S03575
display id: BBa_S03575
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034:J32020
creator: Austen Heinz
created: 2006-08-31T11:00:00Z
modified: 2015-05-08T01:14:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J32020
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J32020
sequence: https://synbiohub.org/public/igem/BBa_S03575_sequence/1

BBa_J32020
type: Component
persistent identity: component1898434
display id: component1898434
version: 1
top level: BBa_S03575
access: public
definition: https://synbiohub.org/public/igem/BBa_J32020/1

BBa_B0034
type: Component
persistent identity: component1898433
display id: component1898433
version: 1
top level: BBa_S03575
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1898433
display id: annotation1898433
version: 1
top level: BBa_S03575
location: https://synbiohub.org/public/igem/BBa_S03575/annotation1898433/range1898433/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03575/annotation1898433/range1898433/1
type: Range
persistent identity: range1898433
display id: range1898433
version: 1
direction: direction/reverse
top level: BBa_S03575
start: 1
end: 12
orientation: inline

BBa_J32020
type: SequenceAnnotation
persistent identity: annotation1898434
display id: annotation1898434
version: 1
top level: BBa_S03575
location: https://synbiohub.org/public/igem/BBa_S03575/annotation1898434/range1898434/1
component: BBa_J32020

https://synbiohub.org/public/igem/BBa_S03575/annotation1898434/range1898434/1
type: Range
persistent identity: range1898434
display id: range1898434
version: 1
direction: direction/reverse
top level: BBa_S03575
start: 19
end: 869
orientation: inline

BBa_P0451
type: ComponentDefinition
persistent identity: BBa P0451
display id: BBa_P0451
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (lambda) [S0153]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_C0051
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_P0451_sequence/1

BBa_B0034
type: Component
persistent identity: component944957
display id: component944957
version: 1
top level: BBa_P0451
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: Component
persistent identity: component944967
display id: component944967
version: 1
top level: BBa_P0451
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0012
type: Component
persistent identity: component944983
display id: component944983
version: 1
top level: BBa_P0451
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944973
display id: component944973
version: 1
top level: BBa_P0451
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944957
display id: annotation944957
version: 1
top level: BBa_P0451
location: https://synbiohub.org/public/igem/BBa_P0451/annotation944957/range944957/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0451/annotation944957/range944957/1
type: Range
persistent identity: range944957
display id: range944957
version: 1
direction: direction/forward
top level: BBa_P0451
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944983
display id: annotation944983
version: 1
top level: BBa_P0451
location: https://synbiohub.org/public/igem/BBa_P0451/annotation944983/range944983/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0451/annotation944983/range944983/1
type: Range
persistent identity: range944983
display id: range944983
version: 1
direction: direction/forward
top level: BBa_P0451
start: 890
end: 930
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944973
display id: annotation944973
version: 1
top level: BBa_P0451
location: https://synbiohub.org/public/igem/BBa_P0451/annotation944973/range944973/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0451/annotation944973/range944973/1
type: Range
persistent identity: range944973
display id: range944973
version: 1
direction: direction/forward
top level: BBa_P0451
start: 802
end: 881
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation944967
display id: annotation944967
version: 1
top level: BBa_P0451
location: https://synbiohub.org/public/igem/BBa_P0451/annotation944967/range944967/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0451/annotation944967/range944967/1
type: Range
persistent identity: range944967
display id: range944967
version: 1
direction: direction/forward
top level: BBa_P0451
start: 19
end: 768
orientation: inline

BBa_K081015
type: ComponentDefinition
persistent identity: BBa K081015
display id: BBa_K081015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luxI protein generator - PoPS->luxI
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: PoPS in -> luxI out. Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: luxI: T:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_C0061
component: BBa_B1006
sequence annotation: BBa_C0061
sequence annotation: BBa_B1006
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081015_sequence/1

BBa_B0030
type: Component
persistent identity: component2245999
display id: component2245999
version: 1
top level: BBa_K081015
access: public
definition: BBa_B0030

BBa_C0061
type: Component
persistent identity: component2246002
display id: component2246002
version: 1
top level: BBa_K081015
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B1006
type: Component
persistent identity: component2246009
display id: component2246009
version: 1
top level: BBa_K081015
access: public
definition: BBa_B1006

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2246002
display id: annotation2246002
version: 1
top level: BBa_K081015
location: https://synbiohub.org/public/igem/BBa_K081015/annotation2246002/range2246002/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K081015/annotation2246002/range2246002/1
type: Range
persistent identity: range2246002
display id: range2246002
version: 1
direction: direction/forward
top level: BBa_K081015
start: 22
end: 639
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2246009
display id: annotation2246009
version: 1
top level: BBa_K081015
location: https://synbiohub.org/public/igem/BBa_K081015/annotation2246009/range2246009/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081015/annotation2246009/range2246009/1
type: Range
persistent identity: range2246009
display id: range2246009
version: 1
direction: direction/reverse
top level: BBa_K081015
start: 673
end: 711
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2245999
display id: annotation2245999
version: 1
top level: BBa_K081015
location: https://synbiohub.org/public/igem/BBa_K081015/annotation2245999/range2245999/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081015/annotation2245999/range2245999/1
type: Range
persistent identity: range2245999
display id: range2245999
version: 1
direction: direction/forward
top level: BBa_K081015
start: 1
end: 15
orientation: inline

BBa_I732906
type: ComponentDefinition
persistent identity: BBa I732906
display id: BBa_I732906
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [aTc] -> [LRLb]
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_B0012
component: BBa_I732084
component: BBa_R0040
component: BBa_B0010
component: BBa_I732102
sequence annotation: BBa_I732084
sequence annotation: BBa_I732102
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732906_sequence/1

BBa_B0034
type: Component
persistent identity: component1948659
display id: component1948659
version: 1
top level: BBa_I732906
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1948649
display id: component1948649
version: 1
top level: BBa_I732906
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I732084
type: Component
persistent identity: component1948646
display id: component1948646
version: 1
top level: BBa_I732906
access: public
definition: https://synbiohub.org/public/igem/BBa_I732084/1

BBa_R0040
type: Component
persistent identity: component1948654
display id: component1948654
version: 1
top level: BBa_I732906
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1948647
display id: component1948647
version: 1
top level: BBa_I732906
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I732102
type: Component
persistent identity: component1948660
display id: component1948660
version: 1
top level: BBa_I732906
access: public
definition: https://synbiohub.org/public/igem/BBa_I732102/1

BBa_I732084
type: SequenceAnnotation
persistent identity: annotation1948646
display id: annotation1948646
version: 1
top level: BBa_I732906
location: https://synbiohub.org/public/igem/BBa_I732906/annotation1948646/range1948646/1
component: BBa_I732084

https://synbiohub.org/public/igem/BBa_I732906/annotation1948646/range1948646/1
type: Range
persistent identity: range1948646
display id: range1948646
version: 1
direction: direction/reverse
top level: BBa_I732906
start: 1
end: 689
orientation: inline

BBa_I732102
type: SequenceAnnotation
persistent identity: annotation1948660
display id: annotation1948660
version: 1
top level: BBa_I732906
location: https://synbiohub.org/public/igem/BBa_I732906/annotation1948660/range1948660/1
component: BBa_I732102

https://synbiohub.org/public/igem/BBa_I732906/annotation1948660/range1948660/1
type: Range
persistent identity: range1948660
display id: range1948660
version: 1
direction: direction/reverse
top level: BBa_I732906
start: 915
end: 2000
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948649
display id: annotation1948649
version: 1
top level: BBa_I732906
location: https://synbiohub.org/public/igem/BBa_I732906/annotation1948649/range1948649/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732906/annotation1948649/range1948649/1
type: Range
persistent identity: range1948649
display id: range1948649
version: 1
direction: direction/forward
top level: BBa_I732906
start: 786
end: 826
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948647
display id: annotation1948647
version: 1
top level: BBa_I732906
location: https://synbiohub.org/public/igem/BBa_I732906/annotation1948647/range1948647/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732906/annotation1948647/range1948647/1
type: Range
persistent identity: range1948647
display id: range1948647
version: 1
direction: direction/forward
top level: BBa_I732906
start: 698
end: 777
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1948654
display id: annotation1948654
version: 1
top level: BBa_I732906
location: https://synbiohub.org/public/igem/BBa_I732906/annotation1948654/range1948654/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I732906/annotation1948654/range1948654/1
type: Range
persistent identity: range1948654
display id: range1948654
version: 1
direction: direction/forward
top level: BBa_I732906
start: 835
end: 888
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948659
display id: annotation1948659
version: 1
top level: BBa_I732906
location: https://synbiohub.org/public/igem/BBa_I732906/annotation1948659/range1948659/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732906/annotation1948659/range1948659/1
type: Range
persistent identity: range1948659
display id: range1948659
version: 1
direction: direction/reverse
top level: BBa_I732906
start: 897
end: 908
orientation: inline

PenI
type: ComponentDefinition
persistent identity: BBa R0074
display id: BBa_R0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (PenI regulated)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).
mutable notes: extends slightly past -50
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: -35
sequence annotation: dimer right half
sequence annotation: PenI
sequence annotation: dimer left half
sequence: https://synbiohub.org/public/igem/BBa_R0074_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation319212
display id: annotation319212
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319212/range319212/1
type: Range
persistent identity: range319212
display id: range319212
version: 1
direction: direction/forward
top level: PenI
start: 46
end: 51
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation319211
display id: annotation319211
version: 1
top level: PenI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319211/range319211/1
type: Range
persistent identity: range319211
display id: range319211
version: 1
direction: direction/forward
top level: PenI
start: 23
end: 28
orientation: inline

dimer right half
type: SequenceAnnotation
persistent identity: annotation319789
display id: annotation319789
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319789/range319789/1
type: Range
persistent identity: range319789
display id: range319789
version: 1
direction: direction/forward
top level: PenI
start: 55
end: 76
orientation: inline

PenI
type: SequenceAnnotation
persistent identity: annotation302713
display id: annotation302713
version: 1
top level: PenI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1

https://synbiohub.org/public/igem/BBa_R0074/annotation302713/range302713/1
type: Range
persistent identity: range302713
display id: range302713
version: 1
direction: direction/forward
top level: PenI
start: 1
end: 77
orientation: inline

dimer left half
type: SequenceAnnotation
persistent identity: annotation319788
display id: annotation319788
version: 1
top level: PenI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1

https://synbiohub.org/public/igem/BBa_R0074/annotation319788/range319788/1
type: Range
persistent identity: range319788
display id: range319788
version: 1
direction: direction/forward
top level: PenI
start: 31
end: 53
orientation: inline

BBa_I13537
type: ComponentDefinition
persistent identity: BBa I13537
display id: BBa_I13537
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Two promoter screening plasmid v1.0
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_I13457
component: BBa_I13458
component: BBa_E0040
component: BBa_I13453
component: BBa_B0012
component: BBa_B0034
component: BBa_E1010
component: BBa_B0010
sequence annotation: BBa_E1010
sequence annotation: BBa_I13458
sequence annotation: BBa_I13457
sequence annotation: BBa_B0010
sequence annotation: BBa_I13453
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13537_sequence/1

BBa_I13457
type: Component
persistent identity: component1663231
display id: component1663231
version: 1
top level: BBa_I13537
access: public
definition: https://synbiohub.org/public/igem/BBa_I13457/1

BBa_I13458
type: Component
persistent identity: component1663209
display id: component1663209
version: 1
top level: BBa_I13537
access: public
definition: BBa_I13458

BBa_E0040
type: Component
persistent identity: component1663271
display id: component1663271
version: 1
top level: BBa_I13537
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_I13453
type: Component
persistent identity: component1663211
display id: component1663211
version: 1
top level: BBa_I13537
access: public
definition: BBa_I13453

BBa_B0012
type: Component
persistent identity: component1663286
display id: component1663286
version: 1
top level: BBa_I13537
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1663236
display id: component1663236
version: 1
top level: BBa_I13537
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13453
type: Component
persistent identity: component1663264
display id: component1663264
version: 1
top level: BBa_I13537
access: public
definition: BBa_I13453

BBa_B0012
type: Component
persistent identity: component1663259
display id: component1663259
version: 1
top level: BBa_I13537
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1663243
display id: component1663243
version: 1
top level: BBa_I13537
access: public
definition: mRFP1

BBa_B0034
type: Component
persistent identity: component1663269
display id: component1663269
version: 1
top level: BBa_I13537
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1663249
display id: component1663249
version: 1
top level: BBa_I13537
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component1663276
display id: component1663276
version: 1
top level: BBa_I13537
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1663243
display id: annotation1663243
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663243/range1663243/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13537/annotation1663243/range1663243/1
type: Range
persistent identity: range1663243
display id: range1663243
version: 1
direction: direction/forward
top level: BBa_I13537
start: 1398
end: 2078
orientation: inline

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation1663209
display id: annotation1663209
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663209/range1663209/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_I13537/annotation1663209/range1663209/1
type: Range
persistent identity: range1663209
display id: range1663209
version: 1
direction: direction/forward
top level: BBa_I13537
start: 1
end: 1114
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation1663231
display id: annotation1663231
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663231/range1663231/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13537/annotation1663231/range1663231/1
type: Range
persistent identity: range1663231
display id: range1663231
version: 1
direction: direction/forward
top level: BBa_I13537
start: 1261
end: 1371
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663276
display id: annotation1663276
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663276/range1663276/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13537/annotation1663276/range1663276/1
type: Range
persistent identity: range1663276
display id: range1663276
version: 1
direction: direction/forward
top level: BBa_I13537
start: 3133
end: 3212
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1663211
display id: annotation1663211
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663211/range1663211/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13537/annotation1663211/range1663211/1
type: Range
persistent identity: range1663211
display id: range1663211
version: 1
direction: direction/forward
top level: BBa_I13537
start: 1123
end: 1252
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663249
display id: annotation1663249
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663249/range1663249/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13537/annotation1663249/range1663249/1
type: Range
persistent identity: range1663249
display id: range1663249
version: 1
direction: direction/forward
top level: BBa_I13537
start: 2112
end: 2191
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1663271
display id: annotation1663271
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663271/range1663271/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13537/annotation1663271/range1663271/1
type: Range
persistent identity: range1663271
display id: range1663271
version: 1
direction: direction/forward
top level: BBa_I13537
start: 2405
end: 3124
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1663286
display id: annotation1663286
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663286/range1663286/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13537/annotation1663286/range1663286/1
type: Range
persistent identity: range1663286
display id: range1663286
version: 1
direction: direction/forward
top level: BBa_I13537
start: 3221
end: 3261
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663269
display id: annotation1663269
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663269/range1663269/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13537/annotation1663269/range1663269/1
type: Range
persistent identity: range1663269
display id: range1663269
version: 1
direction: direction/forward
top level: BBa_I13537
start: 2387
end: 2398
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663236
display id: annotation1663236
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663236/range1663236/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13537/annotation1663236/range1663236/1
type: Range
persistent identity: range1663236
display id: range1663236
version: 1
direction: direction/forward
top level: BBa_I13537
start: 1380
end: 1391
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1663264
display id: annotation1663264
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663264/range1663264/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13537/annotation1663264/range1663264/1
type: Range
persistent identity: range1663264
display id: range1663264
version: 1
direction: direction/forward
top level: BBa_I13537
start: 2249
end: 2378
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1663259
display id: annotation1663259
version: 1
top level: BBa_I13537
location: https://synbiohub.org/public/igem/BBa_I13537/annotation1663259/range1663259/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13537/annotation1663259/range1663259/1
type: Range
persistent identity: range1663259
display id: range1663259
version: 1
direction: direction/forward
top level: BBa_I13537
start: 2200
end: 2240
orientation: inline

BBa_B1009
type: ComponentDefinition
persistent identity: BBa B1009
display id: BBa_B1009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~=40%)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~>90% * 8bp stem, 6nt loop * Bidirectional, estimated reverse %T~>90%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 4 residues
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1009
sequence annotation: PolyA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1009_sequence/1

B1009
type: SequenceAnnotation
persistent identity: annotation1901009
display id: annotation1901009
version: 1
top level: BBa_B1009
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901009/range1901009/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901009/range1901009/1
type: Range
persistent identity: range1901009
display id: range1901009
version: 1
direction: direction/forward
top level: BBa_B1009
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901011
display id: annotation1901011
version: 1
top level: BBa_B1009
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901011/range1901011/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901011/range1901011/1
type: Range
persistent identity: range1901011
display id: range1901011
version: 1
direction: direction/reverse
top level: BBa_B1009
start: 6
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1901010
display id: annotation1901010
version: 1
top level: BBa_B1009
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901010/range1901010/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901010/range1901010/1
type: Range
persistent identity: range1901010
display id: range1901010
version: 1
direction: direction/forward
top level: BBa_B1009
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901012
display id: annotation1901012
version: 1
top level: BBa_B1009
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1009/annotation1901012/range1901012/1

https://synbiohub.org/public/igem/BBa_B1009/annotation1901012/range1901012/1
type: Range
persistent identity: range1901012
display id: range1901012
version: 1
direction: direction/forward
top level: BBa_B1009
start: 32
end: 35
orientation: inline

rbs+COMT
type: ComponentDefinition
persistent identity: BBa I742109
display id: BBa_I742109
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: COMT gene with ribosome binding site
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: COMT (caffeic acid-O-methyl transferase) with ribosome binding site. Enzyme methylates -OH on C4 of the caffeic acid aromatic ring to produce ferulic acid. COMT is the third gene in the vanillin biosynthesis pathway,
mutable notes: Used COMT cDNA as genomic DNA contains introns
mutable provenance: Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742107
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_I742107
sequence: https://synbiohub.org/public/igem/BBa_I742109_sequence/1

BBa_I742107
type: Component
persistent identity: component1949771
display id: component1949771
version: 1
top level: rbs+COMT
access: public
definition: COMT

BBa_J15001
type: Component
persistent identity: component1949770
display id: component1949770
version: 1
top level: rbs+COMT
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1949770
display id: annotation1949770
version: 1
top level: rbs+COMT
location: https://synbiohub.org/public/igem/BBa_I742109/annotation1949770/range1949770/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_I742109/annotation1949770/range1949770/1
type: Range
persistent identity: range1949770
display id: range1949770
version: 1
direction: direction/reverse
top level: rbs+COMT
start: 1
end: 10
orientation: inline

BBa_I742107
type: SequenceAnnotation
persistent identity: annotation1949771
display id: annotation1949771
version: 1
top level: rbs+COMT
location: https://synbiohub.org/public/igem/BBa_I742109/annotation1949771/range1949771/1
component: BBa_I742107

https://synbiohub.org/public/igem/BBa_I742109/annotation1949771/range1949771/1
type: Range
persistent identity: range1949771
display id: range1949771
version: 1
direction: direction/reverse
top level: rbs+COMT
start: 17
end: 1117
orientation: inline

BBa_I732081
type: ComponentDefinition
persistent identity: BBa I732081
display id: BBa_I732081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressor (RBS+ LVA- TERM-)
creator: Zhan Jian
created: 2007-08-02T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_I732080
sequence annotation: BBa_B0034
sequence annotation: BBa_I732080
sequence: https://synbiohub.org/public/igem/BBa_I732081_sequence/1

BBa_B0034
type: Component
persistent identity: component1940758
display id: component1940758
version: 1
top level: BBa_I732081
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732080
type: Component
persistent identity: component1940760
display id: component1940760
version: 1
top level: BBa_I732081
access: public
definition: https://synbiohub.org/public/igem/BBa_I732080/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940758
display id: annotation1940758
version: 1
top level: BBa_I732081
location: https://synbiohub.org/public/igem/BBa_I732081/annotation1940758/range1940758/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732081/annotation1940758/range1940758/1
type: Range
persistent identity: range1940758
display id: range1940758
version: 1
direction: direction/reverse
top level: BBa_I732081
start: 1
end: 12
orientation: inline

BBa_I732080
type: SequenceAnnotation
persistent identity: annotation1940760
display id: annotation1940760
version: 1
top level: BBa_I732081
location: https://synbiohub.org/public/igem/BBa_I732081/annotation1940760/range1940760/1
component: BBa_I732080

https://synbiohub.org/public/igem/BBa_I732081/annotation1940760/range1940760/1
type: Range
persistent identity: range1940760
display id: range1940760
version: 1
direction: direction/reverse
top level: BBa_I732081
start: 19
end: 645
orientation: inline

BBa_I13458
type: ComponentDefinition
persistent identity: BBa I13458
display id: BBa_I13458
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter C and arabinose C operon
creator: Josh Michener
created: 2005-08-16T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PC and AraC from the AraBAD operator. PC and AraC are on the complementary strand, reading right to left.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_I13458_sequence/1

BBa_P0353
type: ComponentDefinition
persistent identity: BBa P0353
display id: BBa_P0353
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cII (p22) [S0161]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_B0033
component: BBa_C0053
sequence annotation: BBa_C0053
sequence annotation: BBa_B0012
sequence annotation: BBa_B0033
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_P0353_sequence/1

BBa_B0012
type: Component
persistent identity: component944821
display id: component944821
version: 1
top level: BBa_P0353
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944811
display id: component944811
version: 1
top level: BBa_P0353
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0033
type: Component
persistent identity: component944790
display id: component944790
version: 1
top level: BBa_P0353
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_C0053
type: Component
persistent identity: component944803
display id: component944803
version: 1
top level: BBa_P0353
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation944803
display id: annotation944803
version: 1
top level: BBa_P0353
location: https://synbiohub.org/public/igem/BBa_P0353/annotation944803/range944803/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_P0353/annotation944803/range944803/1
type: Range
persistent identity: range944803
display id: range944803
version: 1
direction: direction/forward
top level: BBa_P0353
start: 18
end: 704
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944821
display id: annotation944821
version: 1
top level: BBa_P0353
location: https://synbiohub.org/public/igem/BBa_P0353/annotation944821/range944821/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0353/annotation944821/range944821/1
type: Range
persistent identity: range944821
display id: range944821
version: 1
direction: direction/forward
top level: BBa_P0353
start: 826
end: 866
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation944790
display id: annotation944790
version: 1
top level: BBa_P0353
location: https://synbiohub.org/public/igem/BBa_P0353/annotation944790/range944790/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_P0353/annotation944790/range944790/1
type: Range
persistent identity: range944790
display id: range944790
version: 1
direction: direction/forward
top level: BBa_P0353
start: 1
end: 11
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944811
display id: annotation944811
version: 1
top level: BBa_P0353
location: https://synbiohub.org/public/igem/BBa_P0353/annotation944811/range944811/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0353/annotation944811/range944811/1
type: Range
persistent identity: range944811
display id: range944811
version: 1
direction: direction/forward
top level: BBa_P0353
start: 738
end: 817
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: araC
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
direction: direction/forward
top level: araC
start: 1
end: 876
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
direction: direction/forward
top level: araC
start: 877
end: 909
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
direction: direction/forward
top level: araC
start: 916
end: 940
orientation: inline

BBa_J33203
type: ComponentDefinition
persistent identity: BBa J33203
display id: BBa_J33203
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E. coli ars promoter, arsR gene and lacZ
creator: Chris French
created: 2006-10-12T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This biobrick contains the promoter region of the Escherichia coli chromosomal arsenic detoxification opern, including the arsR gene encoding the ArsR repressor protein required for inducibility of this promoter. This is fused to a lacZ' minigene encoding the N-terminal 77 amino acid residues of LacZ, sufficient to complement the lacZ-delta-M15 mutation. When introduced into an E. coli host strain carrying the lacZ-delta-M15 mutation, this construct results in lacZ expression in the presence of arsenate or arsenite anion. LacZ expression can be detected in various ways. This construct was designed for the detection of arsenate and arsenite in water samples.
mutable notes: Since this construct includes the arsR gene encoding the ArsR repressor which regulates the ars promoter, it requires no other promoter-specific functions. However, it will only give LacZ activity in a host expressing the E. coli lacZ-delta-M15 gene.
mutable provenance: The ars promoter and arsR gene are derived from E. coli JM109 chromosomal DNA. The lacZ' minigene is derived from E. coli BL21 chromosomal DNA, since JM109 does not possess an intact lacZ gene.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_J33201
component: BBa_J33202
sequence annotation: BBa_J33202
sequence annotation: BBa_J33201
sequence: https://synbiohub.org/public/igem/BBa_J33203_sequence/1

BBa_J33201
type: Component
persistent identity: component1902826
display id: component1902826
version: 1
top level: BBa_J33203
access: public
definition: BBa_J33201

BBa_J33202
type: Component
persistent identity: component1902829
display id: component1902829
version: 1
top level: BBa_J33203
access: public
definition: BBa_J33202

BBa_J33202
type: SequenceAnnotation
persistent identity: annotation1902829
display id: annotation1902829
version: 1
top level: BBa_J33203
location: https://synbiohub.org/public/igem/BBa_J33203/annotation1902829/range1902829/1
component: BBa_J33202

https://synbiohub.org/public/igem/BBa_J33203/annotation1902829/range1902829/1
type: Range
persistent identity: range1902829
display id: range1902829
version: 1
direction: direction/reverse
top level: BBa_J33203
start: 527
end: 769
orientation: inline

BBa_J33201
type: SequenceAnnotation
persistent identity: annotation1902826
display id: annotation1902826
version: 1
top level: BBa_J33203
location: https://synbiohub.org/public/igem/BBa_J33203/annotation1902826/range1902826/1
component: BBa_J33201

https://synbiohub.org/public/igem/BBa_J33203/annotation1902826/range1902826/1
type: Range
persistent identity: range1902826
display id: range1902826
version: 1
direction: direction/reverse
top level: BBa_J33203
start: 1
end: 518
orientation: inline

BBa_I12036
type: ComponentDefinition
persistent identity: BBa I12036
display id: BBa_I12036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lamdba Prm promoter (cooperative repression by 434 cI)
creator: Hans
created: 2004-08-17T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operators are used for both activation and repression to enhance cooperativity.
mutable notes: The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage.
mutable provenance: Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 lambda
sequence annotation: -35
sequence annotation: OR1 434
sequence annotation: -10
sequence annotation: OR2 434
sequence annotation: OR2 lambda
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I12036_sequence/1

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation1056735
display id: annotation1056735
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056735/range1056735/1
type: Range
persistent identity: range1056735
display id: range1056735
version: 1
direction: direction/forward
top level: BBa_I12036
start: 9
end: 25
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1056739
display id: annotation1056739
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056739/range1056739/1
type: Range
persistent identity: range1056739
display id: range1056739
version: 1
direction: direction/forward
top level: BBa_I12036
start: 48
end: 53
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation1056737
display id: annotation1056737
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056737/range1056737/1
type: Range
persistent identity: range1056737
display id: range1056737
version: 1
direction: direction/forward
top level: BBa_I12036
start: 56
end: 69
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1056740
display id: annotation1056740
version: 1
top level: BBa_I12036
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056740/range1056740/1
type: Range
persistent identity: range1056740
display id: range1056740
version: 1
direction: direction/forward
top level: BBa_I12036
start: 71
end: 76
orientation: inline

OR2 434
type: SequenceAnnotation
persistent identity: annotation1056738
display id: annotation1056738
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056738/range1056738/1
type: Range
persistent identity: range1056738
display id: range1056738
version: 1
direction: direction/forward
top level: BBa_I12036
start: 78
end: 91
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation1056736
display id: annotation1056736
version: 1
top level: BBa_I12036
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1056736/range1056736/1
type: Range
persistent identity: range1056736
display id: range1056736
version: 1
direction: direction/forward
top level: BBa_I12036
start: 33
end: 49
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1062203
display id: annotation1062203
version: 1
top level: BBa_I12036
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1

https://synbiohub.org/public/igem/BBa_I12036/annotation1062203/range1062203/1
type: Range
persistent identity: range1062203
display id: range1062203
version: 1
direction: direction/forward
top level: BBa_I12036
start: 83
end: 83
orientation: inline

BBa_K118021
type: ComponentDefinition
persistent identity: BBa K118021
display id: BBa_K118021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcstA+rbs+xylE
creator: Andrew Hall
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: ''xylE'' is from the ''Pseudomonas putida'' TOL (naphthalene and xylene degradadative plasmid) pWW0. This gene encodes the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This is a useful reporter gene; colonies or broths expressing active XylE, in the presence of oxygen, will rapidly convert catechol, a cheap colourless substrate, to a bright yellow compound with an absorbance maximum around 377 nm. The part includes the native ribosome binding site, so simply has been added to the glucose-repressible promoter of ''cstA''. This allows for characterisation of pCstA over a range of glucose concentrations.
mutable notes: No special considerations
mutable provenance: The template DNA for ''xylE'' was kindly supplied by Dr. Peter Williams of the University of Wales, Bangor. pCstA is from ''Escherichia coli'' JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_J33204
component: BBa_K118011
sequence annotation: BBa_K118011
sequence annotation: BBa_J33204
sequence: https://synbiohub.org/public/igem/BBa_K118021_sequence/1

BBa_J33204
type: Component
persistent identity: component1978607
display id: component1978607
version: 1
top level: BBa_K118021
access: public
definition: BBa_J33204

BBa_K118011
type: Component
persistent identity: component1978604
display id: component1978604
version: 1
top level: BBa_K118021
access: public
definition: BBa_K118011

BBa_K118011
type: SequenceAnnotation
persistent identity: annotation1978604
display id: annotation1978604
version: 1
top level: BBa_K118021
location: https://synbiohub.org/public/igem/BBa_K118021/annotation1978604/range1978604/1
component: BBa_K118011

https://synbiohub.org/public/igem/BBa_K118021/annotation1978604/range1978604/1
type: Range
persistent identity: range1978604
display id: range1978604
version: 1
direction: direction/reverse
top level: BBa_K118021
start: 1
end: 131
orientation: inline

BBa_J33204
type: SequenceAnnotation
persistent identity: annotation1978607
display id: annotation1978607
version: 1
top level: BBa_K118021
location: https://synbiohub.org/public/igem/BBa_K118021/annotation1978607/range1978607/1
component: BBa_J33204

https://synbiohub.org/public/igem/BBa_K118021/annotation1978607/range1978607/1
type: Range
persistent identity: range1978607
display id: range1978607
version: 1
direction: direction/reverse
top level: BBa_K118021
start: 140
end: 1097
orientation: inline

BBa_K117008
type: ComponentDefinition
persistent identity: BBa K117008
display id: BBa_K117008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLsrA-YFP
creator: Nguyen Xuan Hung
created: 2008-10-07T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is induced by AI-2 to produce YFP.
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_K117002
component: BBa_E0030
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_K117002
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K117008_sequence/1

BBa_B0010
type: Component
persistent identity: component1979968
display id: component1979968
version: 1
top level: BBa_K117008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K117002
type: Component
persistent identity: component1979964
display id: component1979964
version: 1
top level: BBa_K117008
access: public
definition: BBa_K117002

BBa_E0030
type: Component
persistent identity: component1979967
display id: component1979967
version: 1
top level: BBa_K117008
access: public
definition: eyfp

BBa_B0012
type: Component
persistent identity: component1979970
display id: component1979970
version: 1
top level: BBa_K117008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1979966
display id: component1979966
version: 1
top level: BBa_K117008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K117002
type: SequenceAnnotation
persistent identity: annotation1979964
display id: annotation1979964
version: 1
top level: BBa_K117008
location: https://synbiohub.org/public/igem/BBa_K117008/annotation1979964/range1979964/1
component: BBa_K117002

https://synbiohub.org/public/igem/BBa_K117008/annotation1979964/range1979964/1
type: Range
persistent identity: range1979964
display id: range1979964
version: 1
direction: direction/reverse
top level: BBa_K117008
start: 1
end: 102
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1979966
display id: annotation1979966
version: 1
top level: BBa_K117008
location: https://synbiohub.org/public/igem/BBa_K117008/annotation1979966/range1979966/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K117008/annotation1979966/range1979966/1
type: Range
persistent identity: range1979966
display id: range1979966
version: 1
direction: direction/reverse
top level: BBa_K117008
start: 111
end: 122
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1979967
display id: annotation1979967
version: 1
top level: BBa_K117008
location: https://synbiohub.org/public/igem/BBa_K117008/annotation1979967/range1979967/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_K117008/annotation1979967/range1979967/1
type: Range
persistent identity: range1979967
display id: range1979967
version: 1
direction: direction/reverse
top level: BBa_K117008
start: 129
end: 851
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1979968
display id: annotation1979968
version: 1
top level: BBa_K117008
location: https://synbiohub.org/public/igem/BBa_K117008/annotation1979968/range1979968/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K117008/annotation1979968/range1979968/1
type: Range
persistent identity: range1979968
display id: range1979968
version: 1
direction: direction/forward
top level: BBa_K117008
start: 860
end: 939
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1979970
display id: annotation1979970
version: 1
top level: BBa_K117008
location: https://synbiohub.org/public/igem/BBa_K117008/annotation1979970/range1979970/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K117008/annotation1979970/range1979970/1
type: Range
persistent identity: range1979970
display id: range1979970
version: 1
direction: direction/forward
top level: BBa_K117008
start: 948
end: 988
orientation: inline

BBa_I714036
type: ComponentDefinition
persistent identity: BBa I714036
display id: BBa_I714036
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Riboregulator key 1
creator: Chunbo Lou
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
group u list: _142_
m user id: 0
owner id: 1911
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The same as J01008
mutable notes: The Isaacs key1 riboregulator taR12 is modeled on the hok/sok post-segregational killing method of plasmid R1. It makes 25 base pairs with its counterpart, the riboregulator J01010 (aka "lock 1" or "crR12"). Isaacs documentation (see reference #1): Upon induction of the araC promoter (using 0.25% arabinose solution), the taR12 key activated the corresponding crR12 lock to express the downstream indicator, GFP, to levels of fluorescence 19-fold greater than without taR12 expression. Maximal expression was reached at 70 minutes after addition of arabinose solution, and was maintained after that point.
mutable provenance: J01008
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_I714036_sequence/1

BBa_I715040
type: ComponentDefinition
persistent identity: BBa I715040
display id: BBa_I715040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac-RBS-RFP-RBS-GFP
creator: Will DeLoache
created: 2007-07-31T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is designed to ensure that we can detect both RFP and GFP in the same cell.
mutable notes: This part is in plasmid pSB1A2.
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I715039
component: BBa_E5500
sequence annotation: BBa_E5500
sequence annotation: BBa_I715039
sequence: https://synbiohub.org/public/igem/BBa_I715040_sequence/1

BBa_I715039
type: Component
persistent identity: component2252898
display id: component2252898
version: 1
top level: BBa_I715040
access: public
definition: BBa_I715039

BBa_E5500
type: Component
persistent identity: component2252903
display id: component2252903
version: 1
top level: BBa_I715040
access: public
definition: https://synbiohub.org/public/igem/BBa_E5500/1

BBa_E5500
type: SequenceAnnotation
persistent identity: annotation2252903
display id: annotation2252903
version: 1
top level: BBa_I715040
location: https://synbiohub.org/public/igem/BBa_I715040/annotation2252903/range2252903/1
component: BBa_E5500

https://synbiohub.org/public/igem/BBa_I715040/annotation2252903/range2252903/1
type: Range
persistent identity: range2252903
display id: range2252903
version: 1
direction: direction/reverse
top level: BBa_I715040
start: 941
end: 1681
orientation: inline

BBa_I715039
type: SequenceAnnotation
persistent identity: annotation2252898
display id: annotation2252898
version: 1
top level: BBa_I715040
location: https://synbiohub.org/public/igem/BBa_I715040/annotation2252898/range2252898/1
component: BBa_I715039

https://synbiohub.org/public/igem/BBa_I715040/annotation2252898/range2252898/1
type: Range
persistent identity: range2252898
display id: range2252898
version: 1
direction: direction/reverse
top level: BBa_I715040
start: 1
end: 932
orientation: inline

las TetO
type: ComponentDefinition
persistent identity: BBa I14015
display id: BBa_I14015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Las) TetO
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LasR and 3OC12HSL regulated promoter with a single Tet Operator (binding site for TetR repressor)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: TetR
sequence annotation: -35
sequence annotation: R0079
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14015_sequence/1

TetR
type: SequenceAnnotation
persistent identity: annotation1017910
display id: annotation1017910
version: 1
top level: las TetO
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1
type: Range
persistent identity: range1017910
display id: range1017910
version: 1
direction: direction/forward
top level: las TetO
start: 152
end: 170
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026120
display id: annotation1026120
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1
type: Range
persistent identity: range1026120
display id: range1026120
version: 1
direction: direction/forward
top level: las TetO
start: 117
end: 122
orientation: inline

R0079
type: SequenceAnnotation
persistent identity: annotation1017909
display id: annotation1017909
version: 1
top level: las TetO
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1
type: Range
persistent identity: range1017909
display id: range1017909
version: 1
direction: direction/forward
top level: las TetO
start: 1
end: 151
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026116
display id: annotation1026116
version: 1
top level: las TetO
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1

https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1
type: Range
persistent identity: range1026116
display id: range1026116
version: 1
direction: direction/forward
top level: las TetO
start: 140
end: 145
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Modified lambda Prm promoter (OR-3 obliterated)
creator: Hans
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR2
sequence annotation: -35
sequence annotation: -10
sequence annotation: mutate to obliterate OR3
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
direction: direction/forward
top level: Prm +
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
direction: direction/forward
top level: Prm +
start: 48
end: 53
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
direction: direction/forward
top level: Prm +
start: 71
end: 76
orientation: inline

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
direction: direction/forward
top level: Prm +
start: 59
end: 65
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
direction: direction/forward
top level: Prm +
start: 9
end: 25
orientation: inline

BBa_K200028
type: ComponentDefinition
persistent identity: BBa K200028
display id: BBa_K200028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Protease resistant PAH
creator: Charles Fracchia, Royah Vaezi
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:11:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _298_
m user id: 0
owner id: 5155
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Site directed mutagenesis was carried out to change a glutamine residue to a serine to make the PAH enzyme protease resistant. This will allow safe passage through the stomach and gut in order to carry out function as required by Imperial College's 2009 iGEM project, The E.ncapsulator.
mutable notes: Site directed mutagenesis was carried out to obtain the desired characteristics
mutable provenance: PAH from Homo sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cds
sequence annotation: Ser 16 > Glu
sequence: https://synbiohub.org/public/igem/BBa_K200028_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2052046
display id: annotation2052046
version: 1
top level: BBa_K200028
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K200028/annotation2052046/range2052046/1

https://synbiohub.org/public/igem/BBa_K200028/annotation2052046/range2052046/1
type: Range
persistent identity: range2052046
display id: range2052046
version: 1
direction: direction/forward
top level: BBa_K200028
start: 1
end: 1359
orientation: inline

Ser 16 > Glu
type: SequenceAnnotation
persistent identity: annotation2052045
display id: annotation2052045
version: 1
top level: BBa_K200028
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K200028/annotation2052045/range2052045/1

https://synbiohub.org/public/igem/BBa_K200028/annotation2052045/range2052045/1
type: Range
persistent identity: range2052045
display id: range2052045
version: 1
direction: direction/forward
top level: BBa_K200028
start: 46
end: 48
orientation: inline

BBa_K118018
type: ComponentDefinition
persistent identity: BBa K118018
display id: BBa_K118018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+glgC16 (glgC with G336D substitution)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' (ADP-glucose pyrophosphorylase) from ''Escherichia coli'' JM109 with the substitution G336D, with added ribosome binding site (rbs). This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung ''et al.'', 1986; Meyer ''et al.'', 1988)
mutable notes: No special considerations
mutable provenance: ''Escherichia coli'' JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K118016
component: BBa_J15001
sequence annotation: BBa_J15001
sequence annotation: BBa_K118016
sequence: https://synbiohub.org/public/igem/BBa_K118018_sequence/1

BBa_K118016
type: Component
persistent identity: component1978637
display id: component1978637
version: 1
top level: BBa_K118018
access: public
definition: BBa_K118016

BBa_J15001
type: Component
persistent identity: component1978633
display id: component1978633
version: 1
top level: BBa_K118018
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978633
display id: annotation1978633
version: 1
top level: BBa_K118018
location: https://synbiohub.org/public/igem/BBa_K118018/annotation1978633/range1978633/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118018/annotation1978633/range1978633/1
type: Range
persistent identity: range1978633
display id: range1978633
version: 1
direction: direction/reverse
top level: BBa_K118018
start: 1
end: 10
orientation: inline

BBa_K118016
type: SequenceAnnotation
persistent identity: annotation1978637
display id: annotation1978637
version: 1
top level: BBa_K118018
location: https://synbiohub.org/public/igem/BBa_K118018/annotation1978637/range1978637/1
component: BBa_K118016

https://synbiohub.org/public/igem/BBa_K118018/annotation1978637/range1978637/1
type: Range
persistent identity: range1978637
display id: range1978637
version: 1
direction: direction/reverse
top level: BBa_K118018
start: 17
end: 1315
orientation: inline

BBa_E0420
type: ComponentDefinition
persistent identity: BBa E0420
display id: BBa_E0420
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ECFP (RBS+ LVA- TERM) (B0034.E0020.B0015)
creator: Caitlin Conboy
created: 2004-03-15T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Standard CFP output device w/o LVA tag.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_E0020
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_E0420_sequence/1

BBa_B0012
type: Component
persistent identity: component942797
display id: component942797
version: 1
top level: BBa_E0420
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component942782
display id: component942782
version: 1
top level: BBa_E0420
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component942787
display id: component942787
version: 1
top level: BBa_E0420
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component942780
display id: component942780
version: 1
top level: BBa_E0420
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942787
display id: annotation942787
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942787/range942787/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0420/annotation942787/range942787/1
type: Range
persistent identity: range942787
display id: range942787
version: 1
direction: direction/forward
top level: BBa_E0420
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942797
display id: annotation942797
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942797/range942797/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0420/annotation942797/range942797/1
type: Range
persistent identity: range942797
display id: range942797
version: 1
direction: direction/forward
top level: BBa_E0420
start: 838
end: 878
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation942782
display id: annotation942782
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942782/range942782/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_E0420/annotation942782/range942782/1
type: Range
persistent identity: range942782
display id: range942782
version: 1
direction: direction/forward
top level: BBa_E0420
start: 19
end: 741
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942780
display id: annotation942780
version: 1
top level: BBa_E0420
location: https://synbiohub.org/public/igem/BBa_E0420/annotation942780/range942780/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0420/annotation942780/range942780/1
type: Range
persistent identity: range942780
display id: range942780
version: 1
direction: direction/forward
top level: BBa_E0420
start: 1
end: 12
orientation: inline

BBa_I746372
type: ComponentDefinition
persistent identity: BBa I746372
display id: BBa_I746372
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P2 ogr inducing PP promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the P2 ogr activator on the PF promoter. Note that the I746350 does contain a RBS.
mutable notes: I746313 was put upstream of I746322
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746322
component: BBa_I746313
sequence annotation: BBa_I746313
sequence annotation: BBa_I746322
sequence: https://synbiohub.org/public/igem/BBa_I746372_sequence/1

BBa_I746322
type: Component
persistent identity: component2291756
display id: component2291756
version: 1
top level: BBa_I746372
access: public
definition: https://synbiohub.org/public/igem/BBa_I746322/1

BBa_I746313
type: Component
persistent identity: component2291743
display id: component2291743
version: 1
top level: BBa_I746372
access: public
definition: https://synbiohub.org/public/igem/BBa_I746313/1

BBa_I746313
type: SequenceAnnotation
persistent identity: annotation2291743
display id: annotation2291743
version: 1
top level: BBa_I746372
location: https://synbiohub.org/public/igem/BBa_I746372/annotation2291743/range2291743/1
component: BBa_I746313

https://synbiohub.org/public/igem/BBa_I746372/annotation2291743/range2291743/1
type: Range
persistent identity: range2291743
display id: range2291743
version: 1
direction: direction/reverse
top level: BBa_I746372
start: 1
end: 2324
orientation: inline

BBa_I746322
type: SequenceAnnotation
persistent identity: annotation2291756
display id: annotation2291756
version: 1
top level: BBa_I746372
location: https://synbiohub.org/public/igem/BBa_I746372/annotation2291756/range2291756/1
component: BBa_I746322

https://synbiohub.org/public/igem/BBa_I746372/annotation2291756/range2291756/1
type: Range
persistent identity: range2291756
display id: range2291756
version: 1
direction: direction/reverse
top level: BBa_I746372
start: 2333
end: 3307
orientation: inline

EYFP
type: ComponentDefinition
persistent identity: BBa E0034
display id: BBa_E0034
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: stop
sequence annotation: AAV
sequence: https://synbiohub.org/public/igem/BBa_E0034_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1245671
display id: annotation1245671
version: 1
top level: EYFP
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1

https://synbiohub.org/public/igem/BBa_E0034/annotation1245671/range1245671/1
type: Range
persistent identity: range1245671
display id: range1245671
version: 1
direction: direction/forward
top level: EYFP
start: 757
end: 762
orientation: inline

AAV
type: SequenceAnnotation
persistent identity: annotation1245672
display id: annotation1245672
version: 1
top level: EYFP
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1

https://synbiohub.org/public/igem/BBa_E0034/annotation1245672/range1245672/1
type: Range
persistent identity: range1245672
display id: range1245672
version: 1
direction: direction/forward
top level: EYFP
start: 718
end: 756
orientation: inline

BBa_P0140
type: ComponentDefinition
persistent identity: BBa P0140
display id: BBa_P0140
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> TetR [S0163]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein TetR. Used as the input section for Quad Part Inverter Q01140.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0031
component: BBa_B0012
component: BBa_C0040
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0031
sequence annotation: BBa_B0012
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_P0140_sequence/1

BBa_B0031
type: Component
persistent identity: component944432
display id: component944432
version: 1
top level: BBa_P0140
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0012
type: Component
persistent identity: component944458
display id: component944458
version: 1
top level: BBa_P0140
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0040
type: Component
persistent identity: component944442
display id: component944442
version: 1
top level: BBa_P0140
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0010
type: Component
persistent identity: component944448
display id: component944448
version: 1
top level: BBa_P0140
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944448
display id: annotation944448
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944448/range944448/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0140/annotation944448/range944448/1
type: Range
persistent identity: range944448
display id: range944448
version: 1
direction: direction/forward
top level: BBa_P0140
start: 714
end: 793
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944432
display id: annotation944432
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944432/range944432/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0140/annotation944432/range944432/1
type: Range
persistent identity: range944432
display id: range944432
version: 1
direction: direction/forward
top level: BBa_P0140
start: 1
end: 14
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944458
display id: annotation944458
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944458/range944458/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0140/annotation944458/range944458/1
type: Range
persistent identity: range944458
display id: range944458
version: 1
direction: direction/forward
top level: BBa_P0140
start: 802
end: 842
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944442
display id: annotation944442
version: 1
top level: BBa_P0140
location: https://synbiohub.org/public/igem/BBa_P0140/annotation944442/range944442/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0140/annotation944442/range944442/1
type: Range
persistent identity: range944442
display id: range944442
version: 1
direction: direction/forward
top level: BBa_P0140
start: 21
end: 680
orientation: inline

BBa_E0430
type: ComponentDefinition
persistent identity: BBa E0430
display id: BBa_E0430
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EYFP (RBS+ LVA- TERM) (B0034.E0030.B0015)
creator: Caitlin Conboy
created: 2004-03-15T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Standard YFP Output Device -LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_E0430_sequence/1

BBa_B0034
type: Component
persistent identity: component942885
display id: component942885
version: 1
top level: BBa_E0430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component942892
display id: component942892
version: 1
top level: BBa_E0430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component942902
display id: component942902
version: 1
top level: BBa_E0430
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component942887
display id: component942887
version: 1
top level: BBa_E0430
access: public
definition: eyfp

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942885
display id: annotation942885
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942885/range942885/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0430/annotation942885/range942885/1
type: Range
persistent identity: range942885
display id: range942885
version: 1
direction: direction/forward
top level: BBa_E0430
start: 1
end: 12
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation942887
display id: annotation942887
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942887/range942887/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_E0430/annotation942887/range942887/1
type: Range
persistent identity: range942887
display id: range942887
version: 1
direction: direction/forward
top level: BBa_E0430
start: 19
end: 741
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942902
display id: annotation942902
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942902/range942902/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0430/annotation942902/range942902/1
type: Range
persistent identity: range942902
display id: range942902
version: 1
direction: direction/forward
top level: BBa_E0430
start: 838
end: 878
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942892
display id: annotation942892
version: 1
top level: BBa_E0430
location: https://synbiohub.org/public/igem/BBa_E0430/annotation942892/range942892/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0430/annotation942892/range942892/1
type: Range
persistent identity: range942892
display id: range942892
version: 1
direction: direction/forward
top level: BBa_E0430
start: 750
end: 829
orientation: inline

BBa_I13521
type: ComponentDefinition
persistent identity: BBa I13521
display id: BBa_I13521
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ptet mRFP
creator: jkm
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive on mRFP (positive control)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_B0012
component: BBa_R0040
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13521_sequence/1

BBa_E1010
type: Component
persistent identity: component1532573
display id: component1532573
version: 1
top level: BBa_I13521
access: public
definition: mRFP1

BBa_B0012
type: Component
persistent identity: component1532589
display id: component1532589
version: 1
top level: BBa_I13521
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1532558
display id: component1532558
version: 1
top level: BBa_I13521
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1532579
display id: component1532579
version: 1
top level: BBa_I13521
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1532566
display id: component1532566
version: 1
top level: BBa_I13521
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1532573
display id: annotation1532573
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
type: Range
persistent identity: range1532573
display id: range1532573
version: 1
direction: direction/forward
top level: BBa_I13521
start: 81
end: 761
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1532558
display id: annotation1532558
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
type: Range
persistent identity: range1532558
display id: range1532558
version: 1
direction: direction/forward
top level: BBa_I13521
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1532579
display id: annotation1532579
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
type: Range
persistent identity: range1532579
display id: range1532579
version: 1
direction: direction/forward
top level: BBa_I13521
start: 795
end: 874
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1532566
display id: annotation1532566
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
type: Range
persistent identity: range1532566
display id: range1532566
version: 1
direction: direction/forward
top level: BBa_I13521
start: 63
end: 74
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1532589
display id: annotation1532589
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
type: Range
persistent identity: range1532589
display id: range1532589
version: 1
direction: direction/forward
top level: BBa_I13521
start: 883
end: 923
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa I15017
display id: BBa_I15017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.EYFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to generate EYFP under the medium strength RBS B0032.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_E0030
sequence annotation: BBa_B0032
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I15017_sequence/1

BBa_B0032
type: Component
persistent identity: component1478419
display id: component1478419
version: 1
top level: ecfp
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E0030
type: Component
persistent identity: component1478422
display id: component1478422
version: 1
top level: ecfp
access: public
definition: eyfp

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478419
display id: annotation1478419
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15017/annotation1478419/range1478419/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15017/annotation1478419/range1478419/1
type: Range
persistent identity: range1478419
display id: range1478419
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 13
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1478422
display id: annotation1478422
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15017/annotation1478422/range1478422/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I15017/annotation1478422/range1478422/1
type: Range
persistent identity: range1478422
display id: range1478422
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 742
orientation: inline

BBa_S01008
type: ComponentDefinition
persistent identity: BBa S01008
display id: BBa_S01008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0070.E0420
creator: Randy Rettberg
created: 2005-06-10T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from Jen 1 tube 79
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_B0010
component: BBa_E0020
component: BBa_B0012
component: BBa_C0070
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0070
sequence: https://synbiohub.org/public/igem/BBa_S01008_sequence/1

BBa_B0034
type: Component
persistent identity: component1511392
display id: component1511392
version: 1
top level: BBa_S01008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1511415
display id: component1511415
version: 1
top level: BBa_S01008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0020
type: Component
persistent identity: component1511410
display id: component1511410
version: 1
top level: BBa_S01008
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0034
type: Component
persistent identity: component1511408
display id: component1511408
version: 1
top level: BBa_S01008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1511425
display id: component1511425
version: 1
top level: BBa_S01008
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0070
type: Component
persistent identity: component1511402
display id: component1511402
version: 1
top level: BBa_S01008
access: public
definition: rhII

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511392
display id: annotation1511392
version: 1
top level: BBa_S01008
location: https://synbiohub.org/public/igem/BBa_S01008/annotation1511392/range1511392/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01008/annotation1511392/range1511392/1
type: Range
persistent identity: range1511392
display id: range1511392
version: 1
direction: direction/forward
top level: BBa_S01008
start: 1
end: 12
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1511410
display id: annotation1511410
version: 1
top level: BBa_S01008
location: https://synbiohub.org/public/igem/BBa_S01008/annotation1511410/range1511410/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_S01008/annotation1511410/range1511410/1
type: Range
persistent identity: range1511410
display id: range1511410
version: 1
direction: direction/forward
top level: BBa_S01008
start: 712
end: 1434
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1511415
display id: annotation1511415
version: 1
top level: BBa_S01008
location: https://synbiohub.org/public/igem/BBa_S01008/annotation1511415/range1511415/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01008/annotation1511415/range1511415/1
type: Range
persistent identity: range1511415
display id: range1511415
version: 1
direction: direction/forward
top level: BBa_S01008
start: 1443
end: 1522
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1511425
display id: annotation1511425
version: 1
top level: BBa_S01008
location: https://synbiohub.org/public/igem/BBa_S01008/annotation1511425/range1511425/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01008/annotation1511425/range1511425/1
type: Range
persistent identity: range1511425
display id: range1511425
version: 1
direction: direction/forward
top level: BBa_S01008
start: 1531
end: 1571
orientation: inline

BBa_C0070
type: SequenceAnnotation
persistent identity: annotation1511402
display id: annotation1511402
version: 1
top level: BBa_S01008
location: https://synbiohub.org/public/igem/BBa_S01008/annotation1511402/range1511402/1
component: BBa_C0070

https://synbiohub.org/public/igem/BBa_S01008/annotation1511402/range1511402/1
type: Range
persistent identity: range1511402
display id: range1511402
version: 1
direction: direction/forward
top level: BBa_S01008
start: 19
end: 660
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511408
display id: annotation1511408
version: 1
top level: BBa_S01008
location: https://synbiohub.org/public/igem/BBa_S01008/annotation1511408/range1511408/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01008/annotation1511408/range1511408/1
type: Range
persistent identity: range1511408
display id: range1511408
version: 1
direction: direction/forward
top level: BBa_S01008
start: 694
end: 705
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa R1050
display id: BBa_R1050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Standard (HK022 cI regulated)
creator: Reshma Shetty, Drew Endy
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [Note: This is the same part as R0050 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS.
mutable notes: Derived from STHK022N . Modified to comply with BBa_S0001: TTGACA-17N-GATACT Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: putative
sequence annotation: -35
sequence annotation: OR1
sequence annotation: BBa_R1050
sequence annotation: OR2
sequence: https://synbiohub.org/public/igem/BBa_R1050_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation2071
display id: annotation2071
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2071/range2071/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2071/range2071/1
type: Range
persistent identity: range2071
display id: range2071
version: 1
direction: direction/forward
top level: cI HK022
start: 31
end: 36
orientation: inline

putative
type: SequenceAnnotation
persistent identity: annotation2070
display id: annotation2070
version: 1
top level: cI HK022
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2070/range2070/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2070/range2070/1
type: Range
persistent identity: range2070
display id: range2070
version: 1
direction: direction/forward
top level: cI HK022
start: 44
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2065
display id: annotation2065
version: 1
top level: cI HK022
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2065/range2065/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2065/range2065/1
type: Range
persistent identity: range2065
display id: range2065
version: 1
direction: direction/forward
top level: cI HK022
start: 8
end: 14
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2067
display id: annotation2067
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2067/range2067/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2067/range2067/1
type: Range
persistent identity: range2067
display id: range2067
version: 1
direction: direction/forward
top level: cI HK022
start: 38
end: 52
orientation: inline

BBa_R1050
type: SequenceAnnotation
persistent identity: annotation7073
display id: annotation7073
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R1050/annotation7073/range7073/1

https://synbiohub.org/public/igem/BBa_R1050/annotation7073/range7073/1
type: Range
persistent identity: range7073
display id: range7073
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 56
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2066
display id: annotation2066
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R1050/annotation2066/range2066/1

https://synbiohub.org/public/igem/BBa_R1050/annotation2066/range2066/1
type: Range
persistent identity: range2066
display id: range2066
version: 1
direction: direction/forward
top level: cI HK022
start: 14
end: 28
orientation: inline

BBa_I746392
type: ComponentDefinition
persistent identity: BBa I746392
display id: BBa_I746392
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phiR73 delta inducing PP promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the phiR73 delta activator on the PP promoter. Note that the I746352 does contain a RBS.
mutable notes: I746315 was put upstream of I746322
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746322
component: BBa_I746315
sequence annotation: BBa_I746322
sequence annotation: BBa_I746315
sequence: https://synbiohub.org/public/igem/BBa_I746392_sequence/1

BBa_I746322
type: Component
persistent identity: component2292109
display id: component2292109
version: 1
top level: BBa_I746392
access: public
definition: https://synbiohub.org/public/igem/BBa_I746322/1

BBa_I746315
type: Component
persistent identity: component2292096
display id: component2292096
version: 1
top level: BBa_I746392
access: public
definition: https://synbiohub.org/public/igem/BBa_I746315/1

BBa_I746322
type: SequenceAnnotation
persistent identity: annotation2292109
display id: annotation2292109
version: 1
top level: BBa_I746392
location: https://synbiohub.org/public/igem/BBa_I746392/annotation2292109/range2292109/1
component: BBa_I746322

https://synbiohub.org/public/igem/BBa_I746392/annotation2292109/range2292109/1
type: Range
persistent identity: range2292109
display id: range2292109
version: 1
direction: direction/reverse
top level: BBa_I746392
start: 2360
end: 3334
orientation: inline

BBa_I746315
type: SequenceAnnotation
persistent identity: annotation2292096
display id: annotation2292096
version: 1
top level: BBa_I746392
location: https://synbiohub.org/public/igem/BBa_I746392/annotation2292096/range2292096/1
component: BBa_I746315

https://synbiohub.org/public/igem/BBa_I746392/annotation2292096/range2292096/1
type: Range
persistent identity: range2292096
display id: range2292096
version: 1
direction: direction/reverse
top level: BBa_I746392
start: 1
end: 2351
orientation: inline

BBa_I13263
type: ComponentDefinition
persistent identity: BBa I13263
display id: BBa_I13263
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux Receiver (HSL & R0063 driven)
creator: Barry Canton
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Composite part based on the A340620 PoPS amplifier. pLuxL promoter driving luxR-based HSL receiver. pLux-R is driving a YFP output device.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_R0063
component: BBa_C0062
component: BBa_B0010
component: BBa_B0012
component: BBa_R0062
component: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0063
sequence annotation: BBa_B0034
sequence annotation: BBa_R0062
sequence annotation: BBa_E0030
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_I13263_sequence/1

BBa_B0034
type: Component
persistent identity: component1059065
display id: component1059065
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1059121
display id: component1059121
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0063
type: Component
persistent identity: component1059056
display id: component1059056
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_C0062
type: Component
persistent identity: component1059080
display id: component1059080
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0010
type: Component
persistent identity: component1059088
display id: component1059088
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1059098
display id: component1059098
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: Component
persistent identity: component1059113
display id: component1059113
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component1059138
display id: component1059138
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1059128
display id: component1059128
version: 1
top level: BBa_I13263
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component1059123
display id: component1059123
version: 1
top level: BBa_I13263
access: public
definition: eyfp

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1059088
display id: annotation1059088
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059088/range1059088/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13263/annotation1059088/range1059088/1
type: Range
persistent identity: range1059088
display id: range1059088
version: 1
direction: direction/forward
top level: BBa_I13263
start: 967
end: 1046
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1059138
display id: annotation1059138
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059138/range1059138/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13263/annotation1059138/range1059138/1
type: Range
persistent identity: range1059138
display id: range1059138
version: 1
direction: direction/forward
top level: BBa_I13263
start: 2004
end: 2044
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1059056
display id: annotation1059056
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059056/range1059056/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I13263/annotation1059056/range1059056/1
type: Range
persistent identity: range1059056
display id: range1059056
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1
end: 151
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1059121
display id: annotation1059121
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059121/range1059121/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13263/annotation1059121/range1059121/1
type: Range
persistent identity: range1059121
display id: range1059121
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1167
end: 1178
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1059065
display id: annotation1059065
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059065/range1059065/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13263/annotation1059065/range1059065/1
type: Range
persistent identity: range1059065
display id: range1059065
version: 1
direction: direction/forward
top level: BBa_I13263
start: 160
end: 171
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1059113
display id: annotation1059113
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059113/range1059113/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13263/annotation1059113/range1059113/1
type: Range
persistent identity: range1059113
display id: range1059113
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1104
end: 1158
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1059123
display id: annotation1059123
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059123/range1059123/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13263/annotation1059123/range1059123/1
type: Range
persistent identity: range1059123
display id: range1059123
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1185
end: 1907
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1059098
display id: annotation1059098
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059098/range1059098/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13263/annotation1059098/range1059098/1
type: Range
persistent identity: range1059098
display id: range1059098
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1055
end: 1095
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1059080
display id: annotation1059080
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059080/range1059080/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13263/annotation1059080/range1059080/1
type: Range
persistent identity: range1059080
display id: range1059080
version: 1
direction: direction/forward
top level: BBa_I13263
start: 178
end: 933
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1059128
display id: annotation1059128
version: 1
top level: BBa_I13263
location: https://synbiohub.org/public/igem/BBa_I13263/annotation1059128/range1059128/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13263/annotation1059128/range1059128/1
type: Range
persistent identity: range1059128
display id: range1059128
version: 1
direction: direction/forward
top level: BBa_I13263
start: 1916
end: 1995
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: CAP binding site
sequence annotation: -10
sequence annotation: start
sequence annotation: LacI binding site
sequence annotation: -35
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
direction: direction/forward
top level: LacI
start: 89
end: 126
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
direction: direction/forward
top level: LacI
start: 161
end: 166
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
direction: direction/forward
top level: LacI
start: 173
end: 173
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
direction: direction/forward
top level: LacI
start: 166
end: 200
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
direction: direction/forward
top level: LacI
start: 137
end: 142
orientation: inline

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
direction: direction/forward
top level: LacI
start: 1
end: 200
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: promoter P(Lac) IQ
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive Promoter, High Transcription
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: P(Lac) IQ
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 3
end: 8
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 1
end: 37
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
direction: direction/forward
top level: P(Lac) IQ
start: 26
end: 31
orientation: inline

BBa_I13543
type: ComponentDefinition
persistent identity: BBa I13543
display id: BBa_I13543
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intermediate in two promoter screening plasmid
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I0500
component: BBa_I13457
component: BBa_B0010
component: BBa_B0034
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_I13457
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I13543_sequence/1

BBa_I0500
type: Component
persistent identity: component1663959
display id: component1663959
version: 1
top level: BBa_I13543
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_I13457
type: Component
persistent identity: component1663979
display id: component1663979
version: 1
top level: BBa_I13543
access: public
definition: https://synbiohub.org/public/igem/BBa_I13457/1

BBa_B0010
type: Component
persistent identity: component1663997
display id: component1663997
version: 1
top level: BBa_I13543
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1663984
display id: component1663984
version: 1
top level: BBa_I13543
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1663991
display id: component1663991
version: 1
top level: BBa_I13543
access: public
definition: mRFP1

BBa_B0012
type: Component
persistent identity: component1664007
display id: component1664007
version: 1
top level: BBa_I13543
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1663991
display id: annotation1663991
version: 1
top level: BBa_I13543
location: https://synbiohub.org/public/igem/BBa_I13543/annotation1663991/range1663991/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13543/annotation1663991/range1663991/1
type: Range
persistent identity: range1663991
display id: range1663991
version: 1
direction: direction/forward
top level: BBa_I13543
start: 1356
end: 2036
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1664007
display id: annotation1664007
version: 1
top level: BBa_I13543
location: https://synbiohub.org/public/igem/BBa_I13543/annotation1664007/range1664007/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13543/annotation1664007/range1664007/1
type: Range
persistent identity: range1664007
display id: range1664007
version: 1
direction: direction/forward
top level: BBa_I13543
start: 2158
end: 2198
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation1663979
display id: annotation1663979
version: 1
top level: BBa_I13543
location: https://synbiohub.org/public/igem/BBa_I13543/annotation1663979/range1663979/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13543/annotation1663979/range1663979/1
type: Range
persistent identity: range1663979
display id: range1663979
version: 1
direction: direction/forward
top level: BBa_I13543
start: 1219
end: 1329
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663984
display id: annotation1663984
version: 1
top level: BBa_I13543
location: https://synbiohub.org/public/igem/BBa_I13543/annotation1663984/range1663984/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13543/annotation1663984/range1663984/1
type: Range
persistent identity: range1663984
display id: range1663984
version: 1
direction: direction/forward
top level: BBa_I13543
start: 1338
end: 1349
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663997
display id: annotation1663997
version: 1
top level: BBa_I13543
location: https://synbiohub.org/public/igem/BBa_I13543/annotation1663997/range1663997/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13543/annotation1663997/range1663997/1
type: Range
persistent identity: range1663997
display id: range1663997
version: 1
direction: direction/forward
top level: BBa_I13543
start: 2070
end: 2149
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1663959
display id: annotation1663959
version: 1
top level: BBa_I13543
location: https://synbiohub.org/public/igem/BBa_I13543/annotation1663959/range1663959/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13543/annotation1663959/range1663959/1
type: Range
persistent identity: range1663959
display id: range1663959
version: 1
direction: direction/forward
top level: BBa_I13543
start: 1
end: 1210
orientation: inline

BBa_K082003
type: ComponentDefinition
persistent identity: BBa K082003
display id: BBa_K082003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP(+LVA)
creator: Yanting Xue , He Shen
created: 2008-10-27T12:00:00Z
modified: 2016-01-25T04:43:13Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 4206
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: noen
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_K082003_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1993327
display id: annotation1993327
version: 1
top level: BBa_K082003
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_K082003/annotation1993327/range1993327/1

https://synbiohub.org/public/igem/BBa_K082003/annotation1993327/range1993327/1
type: Range
persistent identity: range1993327
display id: range1993327
version: 1
direction: direction/forward
top level: BBa_K082003
start: 721
end: 753
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation1992959
display id: annotation1992959
version: 1
top level: BBa_K082003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082003/annotation1992959/range1992959/1

https://synbiohub.org/public/igem/BBa_K082003/annotation1992959/range1992959/1
type: Range
persistent identity: range1992959
display id: range1992959
version: 1
direction: direction/forward
top level: BBa_K082003
start: 1
end: 720
orientation: inline

BBa_I732904
type: ComponentDefinition
persistent identity: BBa I732904
display id: BBa_I732904
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040 B0015
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0040
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I732904_sequence/1

BBa_R0040
type: Component
persistent identity: component1948588
display id: component1948588
version: 1
top level: BBa_I732904
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1948592
display id: component1948592
version: 1
top level: BBa_I732904
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1948594
display id: component1948594
version: 1
top level: BBa_I732904
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948592
display id: annotation1948592
version: 1
top level: BBa_I732904
location: https://synbiohub.org/public/igem/BBa_I732904/annotation1948592/range1948592/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732904/annotation1948592/range1948592/1
type: Range
persistent identity: range1948592
display id: range1948592
version: 1
direction: direction/forward
top level: BBa_I732904
start: 63
end: 142
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948594
display id: annotation1948594
version: 1
top level: BBa_I732904
location: https://synbiohub.org/public/igem/BBa_I732904/annotation1948594/range1948594/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732904/annotation1948594/range1948594/1
type: Range
persistent identity: range1948594
display id: range1948594
version: 1
direction: direction/forward
top level: BBa_I732904
start: 151
end: 191
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1948588
display id: annotation1948588
version: 1
top level: BBa_I732904
location: https://synbiohub.org/public/igem/BBa_I732904/annotation1948588/range1948588/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I732904/annotation1948588/range1948588/1
type: Range
persistent identity: range1948588
display id: range1948588
version: 1
direction: direction/forward
top level: BBa_I732904
start: 1
end: 54
orientation: inline

rbs+crtZ
type: ComponentDefinition
persistent identity: BBa I742158
display id: BBa_I742158
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtZ (beta-carotene hydroxylase) with native rbs
creator: Chris French
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxnathin.
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt combination to generate the final sequence.
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_I742156
component: BBa_I742157
sequence annotation: BBa_I742156
sequence annotation: BBa_I742157
sequence: https://synbiohub.org/public/igem/BBa_I742158_sequence/1

BBa_I742156
type: Component
persistent identity: component1953813
display id: component1953813
version: 1
top level: rbs+crtZ
access: public
definition: https://synbiohub.org/public/igem/BBa_I742156/1

BBa_I742157
type: Component
persistent identity: component1953815
display id: component1953815
version: 1
top level: rbs+crtZ
access: public
definition: https://synbiohub.org/public/igem/BBa_I742157/1

BBa_I742156
type: SequenceAnnotation
persistent identity: annotation1953813
display id: annotation1953813
version: 1
top level: rbs+crtZ
location: https://synbiohub.org/public/igem/BBa_I742158/annotation1953813/range1953813/1
component: BBa_I742156

https://synbiohub.org/public/igem/BBa_I742158/annotation1953813/range1953813/1
type: Range
persistent identity: range1953813
display id: range1953813
version: 1
direction: direction/reverse
top level: rbs+crtZ
start: 1
end: 16
orientation: inline

BBa_I742157
type: SequenceAnnotation
persistent identity: annotation1953815
display id: annotation1953815
version: 1
top level: rbs+crtZ
location: https://synbiohub.org/public/igem/BBa_I742158/annotation1953815/range1953815/1
component: BBa_I742157

https://synbiohub.org/public/igem/BBa_I742158/annotation1953815/range1953815/1
type: Range
persistent identity: range1953815
display id: range1953815
version: 1
direction: direction/reverse
top level: rbs+crtZ
start: 17
end: 547
orientation: inline

BBa_I732097
type: ComponentDefinition
persistent identity: BBa I732097
display id: BBa_I732097
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Activity Reporter (RFP)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter can be inserted into the adapter containing BglII and BamHI sites. The activity of inserted promoter can be quantificationally measured using ONPG assay or fluorescent assay.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E1010
component: BBa_I732007
component: BBa_B0015
component: BBa_B0034
sequence annotation: BBa_I732007
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732097_sequence/1

BBa_E1010
type: Component
persistent identity: component2253303
display id: component2253303
version: 1
top level: BBa_I732097
access: public
definition: mRFP1

BBa_I732007
type: Component
persistent identity: component2253298
display id: component2253298
version: 1
top level: BBa_I732097
access: public
definition: Adapter

BBa_B0015
type: Component
persistent identity: component2253310
display id: component2253310
version: 1
top level: BBa_I732097
access: public
definition: BBa_B0015

BBa_B0034
type: Component
persistent identity: component2253300
display id: component2253300
version: 1
top level: BBa_I732097
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation2253298
display id: annotation2253298
version: 1
top level: BBa_I732097
location: https://synbiohub.org/public/igem/BBa_I732097/annotation2253298/range2253298/1
component: BBa_I732007

https://synbiohub.org/public/igem/BBa_I732097/annotation2253298/range2253298/1
type: Range
persistent identity: range2253298
display id: range2253298
version: 1
direction: direction/reverse
top level: BBa_I732097
start: 1
end: 19
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2253303
display id: annotation2253303
version: 1
top level: BBa_I732097
location: https://synbiohub.org/public/igem/BBa_I732097/annotation2253303/range2253303/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I732097/annotation2253303/range2253303/1
type: Range
persistent identity: range2253303
display id: range2253303
version: 1
direction: direction/reverse
top level: BBa_I732097
start: 46
end: 751
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2253310
display id: annotation2253310
version: 1
top level: BBa_I732097
location: https://synbiohub.org/public/igem/BBa_I732097/annotation2253310/range2253310/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_I732097/annotation2253310/range2253310/1
type: Range
persistent identity: range2253310
display id: range2253310
version: 1
direction: direction/reverse
top level: BBa_I732097
start: 760
end: 888
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2253300
display id: annotation2253300
version: 1
top level: BBa_I732097
location: https://synbiohub.org/public/igem/BBa_I732097/annotation2253300/range2253300/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732097/annotation2253300/range2253300/1
type: Range
persistent identity: range2253300
display id: range2253300
version: 1
direction: direction/reverse
top level: BBa_I732097
start: 28
end: 39
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa E2060
display id: BBa_E2060
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: derivative of mRFP1, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP (DsRed) derived, Ex587/Em610, yeast codon optimized
mutable notes: Codon optimized for yeast. Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2050 (mOrange), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mOrange (BBa_E2050).
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: GSGTA linker
sequence annotation: mCherry
sequence annotation: Help:Barcodes
sequence annotation: MATSG linker
sequence: https://synbiohub.org/public/igem/BBa_E2060_sequence/1

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431931
display id: annotation1431931
version: 1
top level: mCherry
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1

https://synbiohub.org/public/igem/BBa_E2060/annotation1431931/range1431931/1
type: Range
persistent identity: range1431931
display id: range1431931
version: 1
direction: direction/forward
top level: mCherry
start: 724
end: 738
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation1431932
display id: annotation1431932
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1

https://synbiohub.org/public/igem/BBa_E2060/annotation1431932/range1431932/1
type: Range
persistent identity: range1431932
display id: range1431932
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 738
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214037
display id: annotation2214037
version: 1
top level: mCherry
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1

https://synbiohub.org/public/igem/BBa_E2060/annotation2214037/range2214037/1
type: Range
persistent identity: range2214037
display id: range2214037
version: 1
direction: direction/forward
top level: mCherry
start: 745
end: 769
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431927
display id: annotation1431927
version: 1
top level: mCherry
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1

https://synbiohub.org/public/igem/BBa_E2060/annotation1431927/range1431927/1
type: Range
persistent identity: range1431927
display id: range1431927
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 15
orientation: inline

ARL4A0604
type: ComponentDefinition
persistent identity: BBa I732115
display id: BBa_I732115
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL4A0604
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732115_sequence/1

AraC O2
type: ComponentDefinition
persistent identity: BBa R0081
display id: BBa_R0081
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Inhibitor (AraC loop attachment with O2 site)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: this piece of DNA contains a "dead" part of coding region for AraC, and a functional Operator2 site. when attached to the rest of the promoter region, this part allows for the binding and loop formation that would result in repression of the araC promoter
mutable notes: 1) changed ATG start codon to TAG stop 2) 6 random deletions upstream of putative araC binding sites to preserve spacing for loop back (BB ends will add 6 base pairs); spacing crucial in matching up O2 and I2 binding sites (turns of DNA helix) 3) designed to attach to R0080 to enable all (positive and negative) regulatory functions, and to form "loop back"
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: stem_loop
sequence annotation: defunct araC
sequence: https://synbiohub.org/public/igem/BBa_R0081_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation331787
display id: annotation331787
version: 1
top level: AraC O2
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0081/annotation331787/range331787/1

https://synbiohub.org/public/igem/BBa_R0081/annotation331787/range331787/1
type: Range
persistent identity: range331787
display id: range331787
version: 1
direction: direction/forward
top level: AraC O2
start: 57
end: 72
orientation: inline

defunct araC
type: SequenceAnnotation
persistent identity: annotation331785
display id: annotation331785
version: 1
top level: AraC O2
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_R0081/annotation331785/range331785/1

https://synbiohub.org/public/igem/BBa_R0081/annotation331785/range331785/1
type: Range
persistent identity: range331785
display id: range331785
version: 1
direction: direction/forward
top level: AraC O2
start: 1
end: 78
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation331786
display id: annotation331786
version: 1
top level: AraC O2
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_R0081/annotation331786/range331786/1

https://synbiohub.org/public/igem/BBa_R0081/annotation331786/range331786/1
type: Range
persistent identity: range331786
display id: range331786
version: 1
direction: direction/forward
top level: AraC O2
start: 22
end: 37
orientation: inline

BBa_I746324
type: ComponentDefinition
persistent identity: BBa I746324
display id: BBa_I746324
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP under control of Psid promoter
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is used to screen the level of induction of the Psid promoter (I746364). The Psid promoter is induced by a class of phage activators (P2 ogr: I746350, PSP3pag: I746351, phiR73 delta: I746352).
mutable notes: The Psid promoter was put upstream of I13504.
mutable provenance: registry (I13504) and own construct (I746364)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I746364
component: BBa_B0012
component: BBa_B0034
component: BBa_E0040
component: BBa_B0010
sequence annotation: BBa_I746364
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I746324_sequence/1

BBa_I746364
type: Component
persistent identity: component1943838
display id: component1943838
version: 1
top level: BBa_I746324
access: public
definition: BBa_I746364

BBa_B0012
type: Component
persistent identity: component1943845
display id: component1943845
version: 1
top level: BBa_I746324
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1943840
display id: component1943840
version: 1
top level: BBa_I746324
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1943842
display id: component1943842
version: 1
top level: BBa_I746324
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1943843
display id: component1943843
version: 1
top level: BBa_I746324
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746364
type: SequenceAnnotation
persistent identity: annotation1943838
display id: annotation1943838
version: 1
top level: BBa_I746324
location: https://synbiohub.org/public/igem/BBa_I746324/annotation1943838/range1943838/1
component: BBa_I746364

https://synbiohub.org/public/igem/BBa_I746324/annotation1943838/range1943838/1
type: Range
persistent identity: range1943838
display id: range1943838
version: 1
direction: direction/reverse
top level: BBa_I746324
start: 1
end: 93
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1943842
display id: annotation1943842
version: 1
top level: BBa_I746324
location: https://synbiohub.org/public/igem/BBa_I746324/annotation1943842/range1943842/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I746324/annotation1943842/range1943842/1
type: Range
persistent identity: range1943842
display id: range1943842
version: 1
direction: direction/reverse
top level: BBa_I746324
start: 120
end: 839
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1943845
display id: annotation1943845
version: 1
top level: BBa_I746324
location: https://synbiohub.org/public/igem/BBa_I746324/annotation1943845/range1943845/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746324/annotation1943845/range1943845/1
type: Range
persistent identity: range1943845
display id: range1943845
version: 1
direction: direction/forward
top level: BBa_I746324
start: 936
end: 976
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1943840
display id: annotation1943840
version: 1
top level: BBa_I746324
location: https://synbiohub.org/public/igem/BBa_I746324/annotation1943840/range1943840/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746324/annotation1943840/range1943840/1
type: Range
persistent identity: range1943840
display id: range1943840
version: 1
direction: direction/reverse
top level: BBa_I746324
start: 102
end: 113
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1943843
display id: annotation1943843
version: 1
top level: BBa_I746324
location: https://synbiohub.org/public/igem/BBa_I746324/annotation1943843/range1943843/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746324/annotation1943843/range1943843/1
type: Range
persistent identity: range1943843
display id: range1943843
version: 1
direction: direction/forward
top level: BBa_I746324
start: 848
end: 927
orientation: inline

BBa_F2620
type: ComponentDefinition
persistent identity: BBa F2620
display id: BBa_F2620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL -> PoPS Receiver
creator: Barry Canton [bcanton@mit.edu] and Anna Labno [labnoa@mit.edu]
created: 2004-08-08T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the tet repressible R0040. Initial characterization work has been done on this device using I13273 as a test device.
mutable notes: Example Data Sheets for F2620
mutable provenance: Formerly I13270
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0040
component: BBa_B0034
component: BBa_B0015
component: BBa_R0062
component: BBa_C0062
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence annotation: BBa_R0062
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_F2620_sequence/1

BBa_R0040
type: Component
persistent identity: component2259889
display id: component2259889
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0034
type: Component
persistent identity: component2259895
display id: component2259895
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2259908
display id: component2259908
version: 1
top level: BBa_F2620
access: public
definition: BBa_B0015

BBa_R0062
type: Component
persistent identity: component2259910
display id: component2259910
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_C0062
type: Component
persistent identity: component2259898
display id: component2259898
version: 1
top level: BBa_F2620
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2259895
display id: annotation2259895
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259895/range2259895/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F2620/annotation2259895/range2259895/1
type: Range
persistent identity: range2259895
display id: range2259895
version: 1
direction: direction/reverse
top level: BBa_F2620
start: 63
end: 74
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2259908
display id: annotation2259908
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259908/range2259908/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_F2620/annotation2259908/range2259908/1
type: Range
persistent identity: range2259908
display id: range2259908
version: 1
direction: direction/reverse
top level: BBa_F2620
start: 870
end: 998
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2259889
display id: annotation2259889
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259889/range2259889/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_F2620/annotation2259889/range2259889/1
type: Range
persistent identity: range2259889
display id: range2259889
version: 1
direction: direction/forward
top level: BBa_F2620
start: 1
end: 54
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2259910
display id: annotation2259910
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259910/range2259910/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_F2620/annotation2259910/range2259910/1
type: Range
persistent identity: range2259910
display id: range2259910
version: 1
direction: direction/forward
top level: BBa_F2620
start: 1007
end: 1061
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2259898
display id: annotation2259898
version: 1
top level: BBa_F2620
location: https://synbiohub.org/public/igem/BBa_F2620/annotation2259898/range2259898/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_F2620/annotation2259898/range2259898/1
type: Range
persistent identity: range2259898
display id: range2259898
version: 1
direction: direction/forward
top level: BBa_F2620
start: 81
end: 836
orientation: inline

BBa_E5501
type: ComponentDefinition
persistent identity: BBa E5501
display id: BBa_E5501
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.E0040
creator: jcbraff
created: 2005-05-16T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: B0032.E0040
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E0040
component: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_E5501_sequence/1

BBa_E0040
type: Component
persistent identity: component1489101
display id: component1489101
version: 1
top level: BBa_E5501
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0032
type: Component
persistent identity: component1489098
display id: component1489098
version: 1
top level: BBa_E5501
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1489101
display id: annotation1489101
version: 1
top level: BBa_E5501
location: https://synbiohub.org/public/igem/BBa_E5501/annotation1489101/range1489101/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E5501/annotation1489101/range1489101/1
type: Range
persistent identity: range1489101
display id: range1489101
version: 1
direction: direction/forward
top level: BBa_E5501
start: 20
end: 739
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1489098
display id: annotation1489098
version: 1
top level: BBa_E5501
location: https://synbiohub.org/public/igem/BBa_E5501/annotation1489098/range1489098/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E5501/annotation1489098/range1489098/1
type: Range
persistent identity: range1489098
display id: range1489098
version: 1
direction: direction/forward
top level: BBa_E5501
start: 1
end: 13
orientation: inline

BBa_I13974
type: ComponentDefinition
persistent identity: BBa I13974
display id: BBa_I13974
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: intermediate for lac synchro device
creator: jasonk
created: 2004-10-04T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 2
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: intermediate for lac ring oscillator synchronization device
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0063
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_E0034
component: BBa_C0062
component: BBa_R0051
sequence annotation: BBa_B0012
sequence annotation: BBa_C0062
sequence annotation: BBa_R0051
sequence annotation: BBa_R0063
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0034
sequence: https://synbiohub.org/public/igem/BBa_I13974_sequence/1

BBa_R0063
type: Component
persistent identity: component1246690
display id: component1246690
version: 1
top level: BBa_I13974
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0010
type: Component
persistent identity: component1246663
display id: component1246663
version: 1
top level: BBa_I13974
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1246650
display id: component1246650
version: 1
top level: BBa_I13974
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1246673
display id: component1246673
version: 1
top level: BBa_I13974
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1246732
display id: component1246732
version: 1
top level: BBa_I13974
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0034
type: Component
persistent identity: component1246658
display id: component1246658
version: 1
top level: BBa_I13974
access: public
definition: EYFP

BBa_C0062
type: Component
persistent identity: component1246714
display id: component1246714
version: 1
top level: BBa_I13974
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0010
type: Component
persistent identity: component1246722
display id: component1246722
version: 1
top level: BBa_I13974
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0051
type: Component
persistent identity: component1246642
display id: component1246642
version: 1
top level: BBa_I13974
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0034
type: Component
persistent identity: component1246699
display id: component1246699
version: 1
top level: BBa_I13974
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1246673
display id: annotation1246673
version: 1
top level: BBa_I13974
location: https://synbiohub.org/public/igem/BBa_I13974/annotation1246673/range1246673/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13974/annotation1246673/range1246673/1
type: Range
persistent identity: range1246673
display id: range1246673
version: 1
direction: direction/forward
top level: BBa_I13974
start: 934
end: 974
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1246732
display id: annotation1246732
version: 1
top level: BBa_I13974
location: https://synbiohub.org/public/igem/BBa_I13974/annotation1246732/range1246732/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13974/annotation1246732/range1246732/1
type: Range
persistent identity: range1246732
display id: range1246732
version: 1
direction: direction/forward
top level: BBa_I13974
start: 2037
end: 2077
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1246714
display id: annotation1246714
version: 1
top level: BBa_I13974
location: https://synbiohub.org/public/igem/BBa_I13974/annotation1246714/range1246714/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13974/annotation1246714/range1246714/1
type: Range
persistent identity: range1246714
display id: range1246714
version: 1
direction: direction/forward
top level: BBa_I13974
start: 1160
end: 1915
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1246642
display id: annotation1246642
version: 1
top level: BBa_I13974
location: https://synbiohub.org/public/igem/BBa_I13974/annotation1246642/range1246642/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I13974/annotation1246642/range1246642/1
type: Range
persistent identity: range1246642
display id: range1246642
version: 1
direction: direction/forward
top level: BBa_I13974
start: 1
end: 49
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1246690
display id: annotation1246690
version: 1
top level: BBa_I13974
location: https://synbiohub.org/public/igem/BBa_I13974/annotation1246690/range1246690/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I13974/annotation1246690/range1246690/1
type: Range
persistent identity: range1246690
display id: range1246690
version: 1
direction: direction/forward
top level: BBa_I13974
start: 983
end: 1133
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1246663
display id: annotation1246663
version: 1
top level: BBa_I13974
location: https://synbiohub.org/public/igem/BBa_I13974/annotation1246663/range1246663/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13974/annotation1246663/range1246663/1
type: Range
persistent identity: range1246663
display id: range1246663
version: 1
direction: direction/forward
top level: BBa_I13974
start: 846
end: 925
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1246722
display id: annotation1246722
version: 1
top level: BBa_I13974
location: https://synbiohub.org/public/igem/BBa_I13974/annotation1246722/range1246722/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13974/annotation1246722/range1246722/1
type: Range
persistent identity: range1246722
display id: range1246722
version: 1
direction: direction/forward
top level: BBa_I13974
start: 1949
end: 2028
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1246699
display id: annotation1246699
version: 1
top level: BBa_I13974
location: https://synbiohub.org/public/igem/BBa_I13974/annotation1246699/range1246699/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13974/annotation1246699/range1246699/1
type: Range
persistent identity: range1246699
display id: range1246699
version: 1
direction: direction/forward
top level: BBa_I13974
start: 1142
end: 1153
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1246650
display id: annotation1246650
version: 1
top level: BBa_I13974
location: https://synbiohub.org/public/igem/BBa_I13974/annotation1246650/range1246650/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13974/annotation1246650/range1246650/1
type: Range
persistent identity: range1246650
display id: range1246650
version: 1
direction: direction/forward
top level: BBa_I13974
start: 58
end: 69
orientation: inline

BBa_E0034
type: SequenceAnnotation
persistent identity: annotation1246658
display id: annotation1246658
version: 1
top level: BBa_I13974
location: https://synbiohub.org/public/igem/BBa_I13974/annotation1246658/range1246658/1
component: BBa_E0034

https://synbiohub.org/public/igem/BBa_I13974/annotation1246658/range1246658/1
type: Range
persistent identity: range1246658
display id: range1246658
version: 1
direction: direction/forward
top level: BBa_I13974
start: 76
end: 837
orientation: inline

BBa_J06620
type: ComponentDefinition
persistent identity: BBa J06620
display id: BBa_J06620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Construction intermediate: Lambda cI QPI with EYFP (Q04510.E0430)
creator: ytwang
created: 2005-06-13T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0030
component: BBa_B0012
component: BBa_B0034
component: BBa_C0051
component: BBa_B0010
component: BBa_R0051
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0051
sequence annotation: BBa_C0051
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J06620_sequence/1

BBa_E0030
type: Component
persistent identity: component1534095
display id: component1534095
version: 1
top level: BBa_J06620
access: public
definition: eyfp

BBa_B0012
type: Component
persistent identity: component1534110
display id: component1534110
version: 1
top level: BBa_J06620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1534093
display id: component1534093
version: 1
top level: BBa_J06620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0051
type: Component
persistent identity: component1534054
display id: component1534054
version: 1
top level: BBa_J06620
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0010
type: Component
persistent identity: component1534060
display id: component1534060
version: 1
top level: BBa_J06620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1534044
display id: component1534044
version: 1
top level: BBa_J06620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1534100
display id: component1534100
version: 1
top level: BBa_J06620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0051
type: Component
persistent identity: component1534085
display id: component1534085
version: 1
top level: BBa_J06620
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0012
type: Component
persistent identity: component1534070
display id: component1534070
version: 1
top level: BBa_J06620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1534070
display id: annotation1534070
version: 1
top level: BBa_J06620
location: https://synbiohub.org/public/igem/BBa_J06620/annotation1534070/range1534070/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06620/annotation1534070/range1534070/1
type: Range
persistent identity: range1534070
display id: range1534070
version: 1
direction: direction/forward
top level: BBa_J06620
start: 890
end: 930
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1534044
display id: annotation1534044
version: 1
top level: BBa_J06620
location: https://synbiohub.org/public/igem/BBa_J06620/annotation1534044/range1534044/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06620/annotation1534044/range1534044/1
type: Range
persistent identity: range1534044
display id: range1534044
version: 1
direction: direction/forward
top level: BBa_J06620
start: 1
end: 12
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1534085
display id: annotation1534085
version: 1
top level: BBa_J06620
location: https://synbiohub.org/public/igem/BBa_J06620/annotation1534085/range1534085/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_J06620/annotation1534085/range1534085/1
type: Range
persistent identity: range1534085
display id: range1534085
version: 1
direction: direction/forward
top level: BBa_J06620
start: 939
end: 987
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1534054
display id: annotation1534054
version: 1
top level: BBa_J06620
location: https://synbiohub.org/public/igem/BBa_J06620/annotation1534054/range1534054/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_J06620/annotation1534054/range1534054/1
type: Range
persistent identity: range1534054
display id: range1534054
version: 1
direction: direction/forward
top level: BBa_J06620
start: 19
end: 768
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1534095
display id: annotation1534095
version: 1
top level: BBa_J06620
location: https://synbiohub.org/public/igem/BBa_J06620/annotation1534095/range1534095/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_J06620/annotation1534095/range1534095/1
type: Range
persistent identity: range1534095
display id: range1534095
version: 1
direction: direction/forward
top level: BBa_J06620
start: 1014
end: 1736
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1534093
display id: annotation1534093
version: 1
top level: BBa_J06620
location: https://synbiohub.org/public/igem/BBa_J06620/annotation1534093/range1534093/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06620/annotation1534093/range1534093/1
type: Range
persistent identity: range1534093
display id: range1534093
version: 1
direction: direction/forward
top level: BBa_J06620
start: 996
end: 1007
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1534110
display id: annotation1534110
version: 1
top level: BBa_J06620
location: https://synbiohub.org/public/igem/BBa_J06620/annotation1534110/range1534110/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06620/annotation1534110/range1534110/1
type: Range
persistent identity: range1534110
display id: range1534110
version: 1
direction: direction/forward
top level: BBa_J06620
start: 1833
end: 1873
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1534060
display id: annotation1534060
version: 1
top level: BBa_J06620
location: https://synbiohub.org/public/igem/BBa_J06620/annotation1534060/range1534060/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06620/annotation1534060/range1534060/1
type: Range
persistent identity: range1534060
display id: range1534060
version: 1
direction: direction/forward
top level: BBa_J06620
start: 802
end: 881
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1534100
display id: annotation1534100
version: 1
top level: BBa_J06620
location: https://synbiohub.org/public/igem/BBa_J06620/annotation1534100/range1534100/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06620/annotation1534100/range1534100/1
type: Range
persistent identity: range1534100
display id: range1534100
version: 1
direction: direction/forward
top level: BBa_J06620
start: 1745
end: 1824
orientation: inline

BBa_K081001
type: ComponentDefinition
persistent identity: BBa K081001
display id: BBa_K081001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mux CH1
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device is a PoPS in -> lasI out protein generator with Plas promoter downstream. It is a module of the following genetic multiplexer (mux): {|align="center" | |- | |} where: *PA, PS and PB are three promoters that can be ACTIVATED by the three generic elements A, S and B respectively; *GOI is the ''gene of interest'' that is the output of the system. In particular, '''K081001 is Channel 1'''. People who want to use the genetic mux have to assemble: *K081000 under the desired regulation for Channel 0 (for example the generic promoter PA); *K081001 under the desired regulation for Channel 1 (for example the generic promoter PB); *K081002 under the desired regulation for Selector (for example the generic promoter PS); *the desired protein generator downstream of K081000 and K081001 (for example the generic RBS-GOI-T generator);
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: lasI: T: Plas:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0078
component: BBa_B0030
component: BBa_B1006
component: BBa_R0079
sequence annotation: BBa_C0078
sequence annotation: BBa_R0079
sequence annotation: BBa_B0030
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081001_sequence/1

BBa_C0078
type: Component
persistent identity: component2249344
display id: component2249344
version: 1
top level: BBa_K081001
access: public
definition: lasI

BBa_B0030
type: Component
persistent identity: component2249339
display id: component2249339
version: 1
top level: BBa_K081001
access: public
definition: BBa_B0030

BBa_B1006
type: Component
persistent identity: component2249349
display id: component2249349
version: 1
top level: BBa_K081001
access: public
definition: BBa_B1006

BBa_R0079
type: Component
persistent identity: component2249354
display id: component2249354
version: 1
top level: BBa_K081001
access: public
definition: LasR+PAI

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2249344
display id: annotation2249344
version: 1
top level: BBa_K081001
location: https://synbiohub.org/public/igem/BBa_K081001/annotation2249344/range2249344/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K081001/annotation2249344/range2249344/1
type: Range
persistent identity: range2249344
display id: range2249344
version: 1
direction: direction/reverse
top level: BBa_K081001
start: 22
end: 688
orientation: inline

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2249354
display id: annotation2249354
version: 1
top level: BBa_K081001
location: https://synbiohub.org/public/igem/BBa_K081001/annotation2249354/range2249354/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K081001/annotation2249354/range2249354/1
type: Range
persistent identity: range2249354
display id: range2249354
version: 1
direction: direction/reverse
top level: BBa_K081001
start: 744
end: 900
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249339
display id: annotation2249339
version: 1
top level: BBa_K081001
location: https://synbiohub.org/public/igem/BBa_K081001/annotation2249339/range2249339/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081001/annotation2249339/range2249339/1
type: Range
persistent identity: range2249339
display id: range2249339
version: 1
direction: direction/forward
top level: BBa_K081001
start: 1
end: 15
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2249349
display id: annotation2249349
version: 1
top level: BBa_K081001
location: https://synbiohub.org/public/igem/BBa_K081001/annotation2249349/range2249349/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081001/annotation2249349/range2249349/1
type: Range
persistent identity: range2249349
display id: range2249349
version: 1
direction: direction/reverse
top level: BBa_K081001
start: 697
end: 735
orientation: inline

BBa_I6058
type: ComponentDefinition
persistent identity: BBa I6058
display id: BBa_I6058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test I0500.E0430
creator: Caitlin Conboy and Jennifer Braff
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I0500
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_E0030
sequence annotation: BBa_E0030
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I6058_sequence/1

BBa_I0500
type: Component
persistent identity: component948854
display id: component948854
version: 1
top level: BBa_I6058
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: Component
persistent identity: component948876
display id: component948876
version: 1
top level: BBa_I6058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component948859
display id: component948859
version: 1
top level: BBa_I6058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component948866
display id: component948866
version: 1
top level: BBa_I6058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component948861
display id: component948861
version: 1
top level: BBa_I6058
access: public
definition: eyfp

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation948861
display id: annotation948861
version: 1
top level: BBa_I6058
location: https://synbiohub.org/public/igem/BBa_I6058/annotation948861/range948861/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6058/annotation948861/range948861/1
type: Range
persistent identity: range948861
display id: range948861
version: 1
direction: direction/forward
top level: BBa_I6058
start: 1237
end: 1959
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation948859
display id: annotation948859
version: 1
top level: BBa_I6058
location: https://synbiohub.org/public/igem/BBa_I6058/annotation948859/range948859/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6058/annotation948859/range948859/1
type: Range
persistent identity: range948859
display id: range948859
version: 1
direction: direction/forward
top level: BBa_I6058
start: 1219
end: 1230
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation948854
display id: annotation948854
version: 1
top level: BBa_I6058
location: https://synbiohub.org/public/igem/BBa_I6058/annotation948854/range948854/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I6058/annotation948854/range948854/1
type: Range
persistent identity: range948854
display id: range948854
version: 1
direction: direction/forward
top level: BBa_I6058
start: 1
end: 1210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation948876
display id: annotation948876
version: 1
top level: BBa_I6058
location: https://synbiohub.org/public/igem/BBa_I6058/annotation948876/range948876/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6058/annotation948876/range948876/1
type: Range
persistent identity: range948876
display id: range948876
version: 1
direction: direction/forward
top level: BBa_I6058
start: 2056
end: 2096
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation948866
display id: annotation948866
version: 1
top level: BBa_I6058
location: https://synbiohub.org/public/igem/BBa_I6058/annotation948866/range948866/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6058/annotation948866/range948866/1
type: Range
persistent identity: range948866
display id: range948866
version: 1
direction: direction/forward
top level: BBa_I6058
start: 1968
end: 2047
orientation: inline

BBa_K082006
type: ComponentDefinition
persistent identity: BBa K082006
display id: BBa_K082006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxR-G2F
creator: Yanting Xue , He Shen
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: none
mutable notes: None
mutable provenance: None
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_K082006
sequence: https://synbiohub.org/public/igem/BBa_K082006_sequence/1

BBa_K082006
type: SequenceAnnotation
persistent identity: annotation1993281
display id: annotation1993281
version: 1
top level: BBa_K082006
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_K082006/annotation1993281/range1993281/1

https://synbiohub.org/public/igem/BBa_K082006/annotation1993281/range1993281/1
type: Range
persistent identity: range1993281
display id: range1993281
version: 1
direction: direction/reverse
top level: BBa_K082006
start: 1
end: 753
orientation: inline

BBa_I732912
type: ComponentDefinition
persistent identity: BBa I732912
display id: BBa_I732912
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Adapter + LacI
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_I732100
component: BBa_I732007
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I732100
sequence annotation: BBa_I732007
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I732912_sequence/1

BBa_B0012
type: Component
persistent identity: component1948773
display id: component1948773
version: 1
top level: BBa_I732912
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I732100
type: Component
persistent identity: component1948770
display id: component1948770
version: 1
top level: BBa_I732912
access: public
definition: LacI

BBa_I732007
type: Component
persistent identity: component1948767
display id: component1948767
version: 1
top level: BBa_I732912
access: public
definition: Adapter

BBa_B0010
type: Component
persistent identity: component1948771
display id: component1948771
version: 1
top level: BBa_I732912
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1948769
display id: component1948769
version: 1
top level: BBa_I732912
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948773
display id: annotation1948773
version: 1
top level: BBa_I732912
location: https://synbiohub.org/public/igem/BBa_I732912/annotation1948773/range1948773/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732912/annotation1948773/range1948773/1
type: Range
persistent identity: range1948773
display id: range1948773
version: 1
direction: direction/forward
top level: BBa_I732912
start: 1228
end: 1268
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1948769
display id: annotation1948769
version: 1
top level: BBa_I732912
location: https://synbiohub.org/public/igem/BBa_I732912/annotation1948769/range1948769/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732912/annotation1948769/range1948769/1
type: Range
persistent identity: range1948769
display id: range1948769
version: 1
direction: direction/reverse
top level: BBa_I732912
start: 28
end: 39
orientation: inline

BBa_I732100
type: SequenceAnnotation
persistent identity: annotation1948770
display id: annotation1948770
version: 1
top level: BBa_I732912
location: https://synbiohub.org/public/igem/BBa_I732912/annotation1948770/range1948770/1
component: BBa_I732100

https://synbiohub.org/public/igem/BBa_I732912/annotation1948770/range1948770/1
type: Range
persistent identity: range1948770
display id: range1948770
version: 1
direction: direction/reverse
top level: BBa_I732912
start: 46
end: 1131
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1948767
display id: annotation1948767
version: 1
top level: BBa_I732912
location: https://synbiohub.org/public/igem/BBa_I732912/annotation1948767/range1948767/1
component: BBa_I732007

https://synbiohub.org/public/igem/BBa_I732912/annotation1948767/range1948767/1
type: Range
persistent identity: range1948767
display id: range1948767
version: 1
direction: direction/reverse
top level: BBa_I732912
start: 1
end: 19
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948771
display id: annotation1948771
version: 1
top level: BBa_I732912
location: https://synbiohub.org/public/igem/BBa_I732912/annotation1948771/range1948771/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732912/annotation1948771/range1948771/1
type: Range
persistent identity: range1948771
display id: range1948771
version: 1
direction: direction/forward
top level: BBa_I732912
start: 1140
end: 1219
orientation: inline

RcsB
type: ComponentDefinition
persistent identity: BBa K200000
display id: BBa_K200000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Colanic acid global regulator -> RcsB
creator: James Field
created: 2009-08-11T11:00:00Z
modified: 2015-05-08T01:11:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _298_
m user id: 0
owner id: 5277
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: RcsB is a receiver protein in a two component phosphorelay system. Up-regulation of RcsB has been shown to induce colanic acid production. This is via the activation of the ugd operon which is required for capsule synthesis.
mutable notes: RcsB also upregulates ftsZ which increases the rate of cell division.
mutable provenance: NCBI Reference Sequence: NC_000913.2 >gi|49175990:2314199-2314849
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: stop
sequence annotation: RcsB gene
sequence: https://synbiohub.org/public/igem/BBa_K200000_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2041559
display id: annotation2041559
version: 1
top level: RcsB
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K200000/annotation2041559/range2041559/1

https://synbiohub.org/public/igem/BBa_K200000/annotation2041559/range2041559/1
type: Range
persistent identity: range2041559
display id: range2041559
version: 1
direction: direction/forward
top level: RcsB
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2041560
display id: annotation2041560
version: 1
top level: RcsB
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K200000/annotation2041560/range2041560/1

https://synbiohub.org/public/igem/BBa_K200000/annotation2041560/range2041560/1
type: Range
persistent identity: range2041560
display id: range2041560
version: 1
direction: direction/forward
top level: RcsB
start: 649
end: 653
orientation: inline

RcsB gene
type: SequenceAnnotation
persistent identity: annotation2041558
display id: annotation2041558
version: 1
top level: RcsB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K200000/annotation2041558/range2041558/1

https://synbiohub.org/public/igem/BBa_K200000/annotation2041558/range2041558/1
type: Range
persistent identity: range2041558
display id: range2041558
version: 1
direction: direction/forward
top level: RcsB
start: 1
end: 648
orientation: inline

BBa_B1010
type: ComponentDefinition
persistent identity: BBa B1010
display id: BBa_B1010
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terninator (artificial, large, %T~<10)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~<10% 8bp stem, 6nt loop Bidirectional, estimated reverse %T~=40%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 3 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1010
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1010_sequence/1

B1010
type: SequenceAnnotation
persistent identity: annotation1901013
display id: annotation1901013
version: 1
top level: BBa_B1010
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901013/range1901013/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901013/range1901013/1
type: Range
persistent identity: range1901013
display id: range1901013
version: 1
direction: direction/forward
top level: BBa_B1010
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901015
display id: annotation1901015
version: 1
top level: BBa_B1010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901015/range1901015/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901015/range1901015/1
type: Range
persistent identity: range1901015
display id: range1901015
version: 1
direction: direction/reverse
top level: BBa_B1010
start: 7
end: 9
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901016
display id: annotation1901016
version: 1
top level: BBa_B1010
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901016/range1901016/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901016/range1901016/1
type: Range
persistent identity: range1901016
display id: range1901016
version: 1
direction: direction/forward
top level: BBa_B1010
start: 32
end: 34
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1901014
display id: annotation1901014
version: 1
top level: BBa_B1010
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901014/range1901014/1

https://synbiohub.org/public/igem/BBa_B1010/annotation1901014/range1901014/1
type: Range
persistent identity: range1901014
display id: range1901014
version: 1
direction: direction/forward
top level: BBa_B1010
start: 10
end: 31
orientation: inline

BBa_K145301
type: ComponentDefinition
persistent identity: BBa K145301
display id: BBa_K145301
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacI controlled by key/lock
creator: Nathalie Busschaert
created: 2008-10-20T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _257_
m user id: 0
owner id: 2967
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part can generate the lacI repressor. Normally, however, the production of lacI is inhibited by the presence of the lock. But when key is added, lacI can be generated.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0012
component: BBa_J23032
component: BBa_J23109
component: BBa_B0015
sequence annotation: BBa_C0012
sequence annotation: BBa_B0015
sequence annotation: BBa_J23032
sequence annotation: BBa_J23109
sequence: https://synbiohub.org/public/igem/BBa_K145301_sequence/1

BBa_C0012
type: Component
persistent identity: component2254709
display id: component2254709
version: 1
top level: BBa_K145301
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_J23032
type: Component
persistent identity: component2254707
display id: component2254707
version: 1
top level: BBa_K145301
access: public
definition: https://synbiohub.org/public/igem/BBa_J23032/1

BBa_J23109
type: Component
persistent identity: component2254706
display id: component2254706
version: 1
top level: BBa_K145301
access: public
definition: https://synbiohub.org/public/igem/BBa_J23109/1

BBa_B0015
type: Component
persistent identity: component2254718
display id: component2254718
version: 1
top level: BBa_K145301
access: public
definition: BBa_B0015

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2254709
display id: annotation2254709
version: 1
top level: BBa_K145301
location: https://synbiohub.org/public/igem/BBa_K145301/annotation2254709/range2254709/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K145301/annotation2254709/range2254709/1
type: Range
persistent identity: range2254709
display id: range2254709
version: 1
direction: direction/forward
top level: BBa_K145301
start: 93
end: 1220
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2254718
display id: annotation2254718
version: 1
top level: BBa_K145301
location: https://synbiohub.org/public/igem/BBa_K145301/annotation2254718/range2254718/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K145301/annotation2254718/range2254718/1
type: Range
persistent identity: range2254718
display id: range2254718
version: 1
direction: direction/reverse
top level: BBa_K145301
start: 1254
end: 1382
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation2254707
display id: annotation2254707
version: 1
top level: BBa_K145301
location: https://synbiohub.org/public/igem/BBa_K145301/annotation2254707/range2254707/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K145301/annotation2254707/range2254707/1
type: Range
persistent identity: range2254707
display id: range2254707
version: 1
direction: direction/reverse
top level: BBa_K145301
start: 44
end: 86
orientation: inline

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2254706
display id: annotation2254706
version: 1
top level: BBa_K145301
location: https://synbiohub.org/public/igem/BBa_K145301/annotation2254706/range2254706/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K145301/annotation2254706/range2254706/1
type: Range
persistent identity: range2254706
display id: range2254706
version: 1
direction: direction/reverse
top level: BBa_K145301
start: 1
end: 35
orientation: inline

BBa_I746320
type: ComponentDefinition
persistent identity: BBa I746320
display id: BBa_I746320
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP under control of PF promoter
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is used to screen the level of induction of the PF promoter (I746360). The PF promoter is induced by a class of phage activators (P2 ogr: I746350, PSP3pag: I746351, phiR73 delta: I746352).
mutable notes: The PF promoter was put upstream of I13504.
mutable provenance: registry (I13504) and own construct (I746360)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_I746360
component: BBa_B0012
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I746360
sequence: https://synbiohub.org/public/igem/BBa_I746320_sequence/1

BBa_B0034
type: Component
persistent identity: component1943792
display id: component1943792
version: 1
top level: BBa_I746320
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1943795
display id: component1943795
version: 1
top level: BBa_I746320
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746360
type: Component
persistent identity: component1943790
display id: component1943790
version: 1
top level: BBa_I746320
access: public
definition: BBa_I746360

BBa_B0012
type: Component
persistent identity: component1943797
display id: component1943797
version: 1
top level: BBa_I746320
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1943794
display id: component1943794
version: 1
top level: BBa_I746320
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1943794
display id: annotation1943794
version: 1
top level: BBa_I746320
location: https://synbiohub.org/public/igem/BBa_I746320/annotation1943794/range1943794/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I746320/annotation1943794/range1943794/1
type: Range
persistent identity: range1943794
display id: range1943794
version: 1
direction: direction/reverse
top level: BBa_I746320
start: 118
end: 837
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1943792
display id: annotation1943792
version: 1
top level: BBa_I746320
location: https://synbiohub.org/public/igem/BBa_I746320/annotation1943792/range1943792/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746320/annotation1943792/range1943792/1
type: Range
persistent identity: range1943792
display id: range1943792
version: 1
direction: direction/reverse
top level: BBa_I746320
start: 100
end: 111
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1943795
display id: annotation1943795
version: 1
top level: BBa_I746320
location: https://synbiohub.org/public/igem/BBa_I746320/annotation1943795/range1943795/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746320/annotation1943795/range1943795/1
type: Range
persistent identity: range1943795
display id: range1943795
version: 1
direction: direction/forward
top level: BBa_I746320
start: 846
end: 925
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1943797
display id: annotation1943797
version: 1
top level: BBa_I746320
location: https://synbiohub.org/public/igem/BBa_I746320/annotation1943797/range1943797/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746320/annotation1943797/range1943797/1
type: Range
persistent identity: range1943797
display id: range1943797
version: 1
direction: direction/forward
top level: BBa_I746320
start: 934
end: 974
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation1943790
display id: annotation1943790
version: 1
top level: BBa_I746320
location: https://synbiohub.org/public/igem/BBa_I746320/annotation1943790/range1943790/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_I746320/annotation1943790/range1943790/1
type: Range
persistent identity: range1943790
display id: range1943790
version: 1
direction: direction/reverse
top level: BBa_I746320
start: 1
end: 91
orientation: inline

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: heme oxygenase (ho1) from Synechocystis
creator: Jeff Tabor
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
direction: direction/forward
top level: BBa_I15008
start: 727
end: 751
orientation: inline

BBa_Q04530
type: ComponentDefinition
persistent identity: BBa Q04530
display id: BBa_Q04530
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI (B0034.C0053.B0015.R0053)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: p22 c2 QPI w/ strong RBS
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_R0053
component: BBa_C0053
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_C0053
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0053
sequence: https://synbiohub.org/public/igem/BBa_Q04530_sequence/1

BBa_R0053
type: Component
persistent identity: component953094
display id: component953094
version: 1
top level: BBa_Q04530
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_C0053
type: Component
persistent identity: component953057
display id: component953057
version: 1
top level: BBa_Q04530
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_B0034
type: Component
persistent identity: component953045
display id: component953045
version: 1
top level: BBa_Q04530
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component953075
display id: component953075
version: 1
top level: BBa_Q04530
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component953065
display id: component953065
version: 1
top level: BBa_Q04530
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation953065
display id: annotation953065
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953065/range953065/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04530/annotation953065/range953065/1
type: Range
persistent identity: range953065
display id: range953065
version: 1
direction: direction/forward
top level: BBa_Q04530
start: 739
end: 818
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation953057
display id: annotation953057
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953057/range953057/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_Q04530/annotation953057/range953057/1
type: Range
persistent identity: range953057
display id: range953057
version: 1
direction: direction/forward
top level: BBa_Q04530
start: 19
end: 705
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation953075
display id: annotation953075
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953075/range953075/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04530/annotation953075/range953075/1
type: Range
persistent identity: range953075
display id: range953075
version: 1
direction: direction/forward
top level: BBa_Q04530
start: 827
end: 867
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation953045
display id: annotation953045
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953045/range953045/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04530/annotation953045/range953045/1
type: Range
persistent identity: range953045
display id: range953045
version: 1
direction: direction/forward
top level: BBa_Q04530
start: 1
end: 12
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation953094
display id: annotation953094
version: 1
top level: BBa_Q04530
location: https://synbiohub.org/public/igem/BBa_Q04530/annotation953094/range953094/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_Q04530/annotation953094/range953094/1
type: Range
persistent identity: range953094
display id: range953094
version: 1
direction: direction/forward
top level: BBa_Q04530
start: 876
end: 929
orientation: inline

BBa_S0105
type: ComponentDefinition
persistent identity: BBa S0105
display id: BBa_S0105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0053
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0053
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0053
sequence: https://synbiohub.org/public/igem/BBa_S0105_sequence/1

BBa_C0053
type: Component
persistent identity: component939789
display id: component939789
version: 1
top level: BBa_S0105
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_B0034
type: Component
persistent identity: component939777
display id: component939777
version: 1
top level: BBa_S0105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939777
display id: annotation939777
version: 1
top level: BBa_S0105
location: https://synbiohub.org/public/igem/BBa_S0105/annotation939777/range939777/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0105/annotation939777/range939777/1
type: Range
persistent identity: range939777
display id: range939777
version: 1
direction: direction/forward
top level: BBa_S0105
start: 1
end: 12
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation939789
display id: annotation939789
version: 1
top level: BBa_S0105
location: https://synbiohub.org/public/igem/BBa_S0105/annotation939789/range939789/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_S0105/annotation939789/range939789/1
type: Range
persistent identity: range939789
display id: range939789
version: 1
direction: direction/forward
top level: BBa_S0105
start: 19
end: 705
orientation: inline

RBS-LacY
type: ComponentDefinition
persistent identity: BBa I763015
display id: BBa_I763015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-LacY
creator: Alice Pasini
created: 2007-08-19T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PLac activation induces the expression of the Lactose permase (Lac Y) gene, LacI repressor protein with GFP (+LVA tag) as a reporter.
mutable notes: ---
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J22101
component: BBa_B0034
sequence annotation: BBa_J22101
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I763015_sequence/1

BBa_J22101
type: Component
persistent identity: component1943313
display id: component1943313
version: 1
top level: RBS-LacY
access: public
definition: BBa_J22101

BBa_B0034
type: Component
persistent identity: component1943312
display id: component1943312
version: 1
top level: RBS-LacY
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J22101
type: SequenceAnnotation
persistent identity: annotation1943313
display id: annotation1943313
version: 1
top level: RBS-LacY
location: https://synbiohub.org/public/igem/BBa_I763015/annotation1943313/range1943313/1
component: BBa_J22101

https://synbiohub.org/public/igem/BBa_I763015/annotation1943313/range1943313/1
type: Range
persistent identity: range1943313
display id: range1943313
version: 1
direction: direction/reverse
top level: RBS-LacY
start: 19
end: 1306
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1943312
display id: annotation1943312
version: 1
top level: RBS-LacY
location: https://synbiohub.org/public/igem/BBa_I763015/annotation1943312/range1943312/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I763015/annotation1943312/range1943312/1
type: Range
persistent identity: range1943312
display id: range1943312
version: 1
direction: direction/reverse
top level: RBS-LacY
start: 1
end: 12
orientation: inline

BBa_I732815
type: ComponentDefinition
persistent identity: BBa I732815
display id: BBa_I732815
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL4A0604 (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I732115
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I732115
sequence: https://synbiohub.org/public/igem/BBa_I732815_sequence/1

BBa_I732115
type: Component
persistent identity: component1946208
display id: component1946208
version: 1
top level: BBa_I732815
access: public
definition: ARL4A0604

BBa_B0034
type: Component
persistent identity: component1946207
display id: component1946207
version: 1
top level: BBa_I732815
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946207
display id: annotation1946207
version: 1
top level: BBa_I732815
location: https://synbiohub.org/public/igem/BBa_I732815/annotation1946207/range1946207/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732815/annotation1946207/range1946207/1
type: Range
persistent identity: range1946207
display id: range1946207
version: 1
direction: direction/reverse
top level: BBa_I732815
start: 1
end: 12
orientation: inline

BBa_I732115
type: SequenceAnnotation
persistent identity: annotation1946208
display id: annotation1946208
version: 1
top level: BBa_I732815
location: https://synbiohub.org/public/igem/BBa_I732815/annotation1946208/range1946208/1
component: BBa_I732115

https://synbiohub.org/public/igem/BBa_I732815/annotation1946208/range1946208/1
type: Range
persistent identity: range1946208
display id: range1946208
version: 1
direction: direction/reverse
top level: BBa_I732815
start: 19
end: 1104
orientation: inline

BBa_E2020
type: ComponentDefinition
persistent identity: BBa E2020
display id: BBa_E2020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced version of ECFP, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CFP cerulean, yeast codon optimized.
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Venus YFP (BBa_E2030) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).
mutable provenance: Rizzo MA, et al. (2004) Nat Biot (22):445-9
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Help:Barcodes
sequence annotation: Cerulean CFP
sequence annotation: GSGTA linker
sequence annotation: MATSG linker
sequence: https://synbiohub.org/public/igem/BBa_E2020_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214038
display id: annotation2214038
version: 1
top level: BBa_E2020
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2020/annotation2214038/range2214038/1

https://synbiohub.org/public/igem/BBa_E2020/annotation2214038/range2214038/1
type: Range
persistent identity: range2214038
display id: range2214038
version: 1
direction: direction/forward
top level: BBa_E2020
start: 754
end: 778
orientation: inline

Cerulean CFP
type: SequenceAnnotation
persistent identity: annotation1431974
display id: annotation1431974
version: 1
top level: BBa_E2020
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2020/annotation1431974/range1431974/1

https://synbiohub.org/public/igem/BBa_E2020/annotation1431974/range1431974/1
type: Range
persistent identity: range1431974
display id: range1431974
version: 1
direction: direction/forward
top level: BBa_E2020
start: 1
end: 747
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431973
display id: annotation1431973
version: 1
top level: BBa_E2020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2020/annotation1431973/range1431973/1

https://synbiohub.org/public/igem/BBa_E2020/annotation1431973/range1431973/1
type: Range
persistent identity: range1431973
display id: range1431973
version: 1
direction: direction/forward
top level: BBa_E2020
start: 733
end: 747
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431972
display id: annotation1431972
version: 1
top level: BBa_E2020
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2020/annotation1431972/range1431972/1

https://synbiohub.org/public/igem/BBa_E2020/annotation1431972/range1431972/1
type: Range
persistent identity: range1431972
display id: range1431972
version: 1
direction: direction/forward
top level: BBa_E2020
start: 1
end: 15
orientation: inline

BBa_I13514
type: ComponentDefinition
persistent identity: BBa I13514
display id: BBa_I13514
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 3
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The third screening plasmid. Same as the second except with the flurophores switched. The ends should be capped with I13450.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I0500
component: BBa_B0034
component: BBa_E1010
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_I13514_sequence/1

BBa_I0500
type: Component
persistent identity: component1524851
display id: component1524851
version: 1
top level: BBa_I13514
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component1524863
display id: component1524863
version: 1
top level: BBa_I13514
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1524870
display id: component1524870
version: 1
top level: BBa_I13514
access: public
definition: mRFP1

BBa_E0040
type: Component
persistent identity: component1524858
display id: component1524858
version: 1
top level: BBa_I13514
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: Component
persistent identity: component1524856
display id: component1524856
version: 1
top level: BBa_I13514
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1524876
display id: component1524876
version: 1
top level: BBa_I13514
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1524886
display id: component1524886
version: 1
top level: BBa_I13514
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1524858
display id: annotation1524858
version: 1
top level: BBa_I13514
location: https://synbiohub.org/public/igem/BBa_I13514/annotation1524858/range1524858/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13514/annotation1524858/range1524858/1
type: Range
persistent identity: range1524858
display id: range1524858
version: 1
direction: direction/forward
top level: BBa_I13514
start: 1237
end: 1956
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1524876
display id: annotation1524876
version: 1
top level: BBa_I13514
location: https://synbiohub.org/public/igem/BBa_I13514/annotation1524876/range1524876/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13514/annotation1524876/range1524876/1
type: Range
persistent identity: range1524876
display id: range1524876
version: 1
direction: direction/forward
top level: BBa_I13514
start: 2697
end: 2776
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1524856
display id: annotation1524856
version: 1
top level: BBa_I13514
location: https://synbiohub.org/public/igem/BBa_I13514/annotation1524856/range1524856/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13514/annotation1524856/range1524856/1
type: Range
persistent identity: range1524856
display id: range1524856
version: 1
direction: direction/forward
top level: BBa_I13514
start: 1219
end: 1230
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1524851
display id: annotation1524851
version: 1
top level: BBa_I13514
location: https://synbiohub.org/public/igem/BBa_I13514/annotation1524851/range1524851/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13514/annotation1524851/range1524851/1
type: Range
persistent identity: range1524851
display id: range1524851
version: 1
direction: direction/forward
top level: BBa_I13514
start: 1
end: 1210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1524886
display id: annotation1524886
version: 1
top level: BBa_I13514
location: https://synbiohub.org/public/igem/BBa_I13514/annotation1524886/range1524886/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13514/annotation1524886/range1524886/1
type: Range
persistent identity: range1524886
display id: range1524886
version: 1
direction: direction/forward
top level: BBa_I13514
start: 2785
end: 2825
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1524863
display id: annotation1524863
version: 1
top level: BBa_I13514
location: https://synbiohub.org/public/igem/BBa_I13514/annotation1524863/range1524863/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13514/annotation1524863/range1524863/1
type: Range
persistent identity: range1524863
display id: range1524863
version: 1
direction: direction/forward
top level: BBa_I13514
start: 1965
end: 1976
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1524870
display id: annotation1524870
version: 1
top level: BBa_I13514
location: https://synbiohub.org/public/igem/BBa_I13514/annotation1524870/range1524870/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13514/annotation1524870/range1524870/1
type: Range
persistent identity: range1524870
display id: range1524870
version: 1
direction: direction/forward
top level: BBa_I13514
start: 1983
end: 2663
orientation: inline

lox71
type: ComponentDefinition
persistent identity: BBa I718017
display id: BBa_I718017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lox71
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lox71 is a site specific recombination cassette. It belongs to the loxP family frequently used in genetics, particularly in mouse genetics. lox site recombination is catalysed by a Site specific recombinase, Cre. lox sequences are composed of an 8 bp Core sequence surrounded by two Arms. The particularity of lox66 is that it has an altered sequence at the end of it's left arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm. As a consequence, after a recombination between a lox71 and a lox66 (altered right arm sequence), one of the two resulting generated lox sites has very low recombination potential as it inherited both mutated arms. Use of lox71 & lox66 sites is potentially interesting when the recombination reaction must be "irreversible".
mutable notes: No modifaication was made on lox71 sequence
mutable provenance: This part was generated in the form of a forward & a reverse primer. After annealing these primers EcoRI & PstI compatible cohesive ends at the 5' & 3' ends of the dsDNA were generated. Next, the dsDNA was subcloned in a pSB1A2 open plasmid (digested with EcoRI & PstI) You can follow the construction process by following the links available in the Paris iGEM 2007 wiki: http://parts.mit.edu/igem07/index.php/Paris "freezer" section plasmids table. A links sends you to the corresponding notebook date when the ligation reaction was performed
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I718017_sequence/1

BBa_I13027
type: ComponentDefinition
persistent identity: BBa I13027
display id: BBa_I13027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: penI QPI intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_C0074
sequence annotation: BBa_C0074
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13027_sequence/1

BBa_B0034
type: Component
persistent identity: component939448
display id: component939448
version: 1
top level: BBa_I13027
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0074
type: Component
persistent identity: component939458
display id: component939458
version: 1
top level: BBa_I13027
access: public
definition: penI

BBa_C0074
type: SequenceAnnotation
persistent identity: annotation939458
display id: annotation939458
version: 1
top level: BBa_I13027
location: https://synbiohub.org/public/igem/BBa_I13027/annotation939458/range939458/1
component: BBa_C0074

https://synbiohub.org/public/igem/BBa_I13027/annotation939458/range939458/1
type: Range
persistent identity: range939458
display id: range939458
version: 1
direction: direction/forward
top level: BBa_I13027
start: 19
end: 441
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939448
display id: annotation939448
version: 1
top level: BBa_I13027
location: https://synbiohub.org/public/igem/BBa_I13027/annotation939448/range939448/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13027/annotation939448/range939448/1
type: Range
persistent identity: range939448
display id: range939448
version: 1
direction: direction/forward
top level: BBa_I13027
start: 1
end: 12
orientation: inline

RhlR+C4
type: ComponentDefinition
persistent identity: BBa R0071
display id: BBa_R0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (RhlR & C4-HSL regulated)
creator: Ronny Krashinsky
created: 2004-01-24T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. This is the natural sequence taken from Pseudomonas aeruginosa. Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
mutable notes: The -10 and -35 sites are labeled as identified in [Pearson97]. The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
mutable provenance: Pseudomonas aeruginosa rhlAB promoter
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -10
sequence annotation: RhlR
sequence annotation: -35
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0071_sequence/1

-10
type: SequenceAnnotation
persistent identity: annotation297104
display id: annotation297104
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297104/range297104/1
type: Range
persistent identity: range297104
display id: range297104
version: 1
direction: direction/forward
top level: RhlR+C4
start: 42
end: 47
orientation: inline

RhlR
type: SequenceAnnotation
persistent identity: annotation297102
display id: annotation297102
version: 1
top level: RhlR+C4
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297102/range297102/1
type: Range
persistent identity: range297102
display id: range297102
version: 1
direction: direction/forward
top level: RhlR+C4
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation297103
display id: annotation297103
version: 1
top level: RhlR+C4
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297103/range297103/1
type: Range
persistent identity: range297103
display id: range297103
version: 1
direction: direction/forward
top level: RhlR+C4
start: 19
end: 24
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation297105
display id: annotation297105
version: 1
top level: RhlR+C4
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1

https://synbiohub.org/public/igem/BBa_R0071/annotation297105/range297105/1
type: Range
persistent identity: range297105
display id: range297105
version: 1
direction: direction/forward
top level: RhlR+C4
start: 53
end: 53
orientation: inline

BBa_I714075
type: ComponentDefinition
persistent identity: BBa I714075
display id: BBa_I714075
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [J01008][B0015] ([Key1][Double Terminator])
creator: Mingzhi Qu
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is J01008(key 1) with B0015 double terminator in the end.
mutable provenance: [J01008] + [B0015]
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J01008
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_J01008
sequence: https://synbiohub.org/public/igem/BBa_I714075_sequence/1

BBa_J01008
type: Component
persistent identity: component1952129
display id: component1952129
version: 1
top level: BBa_I714075
access: public
definition: https://synbiohub.org/public/igem/BBa_J01008/1

BBa_B0012
type: Component
persistent identity: component1952132
display id: component1952132
version: 1
top level: BBa_I714075
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1952130
display id: component1952130
version: 1
top level: BBa_I714075
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1952132
display id: annotation1952132
version: 1
top level: BBa_I714075
location: https://synbiohub.org/public/igem/BBa_I714075/annotation1952132/range1952132/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I714075/annotation1952132/range1952132/1
type: Range
persistent identity: range1952132
display id: range1952132
version: 1
direction: direction/forward
top level: BBa_I714075
start: 191
end: 231
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1952130
display id: annotation1952130
version: 1
top level: BBa_I714075
location: https://synbiohub.org/public/igem/BBa_I714075/annotation1952130/range1952130/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I714075/annotation1952130/range1952130/1
type: Range
persistent identity: range1952130
display id: range1952130
version: 1
direction: direction/forward
top level: BBa_I714075
start: 103
end: 182
orientation: inline

BBa_J01008
type: SequenceAnnotation
persistent identity: annotation1952129
display id: annotation1952129
version: 1
top level: BBa_I714075
location: https://synbiohub.org/public/igem/BBa_I714075/annotation1952129/range1952129/1
component: BBa_J01008

https://synbiohub.org/public/igem/BBa_I714075/annotation1952129/range1952129/1
type: Range
persistent identity: range1952129
display id: range1952129
version: 1
direction: direction/reverse
top level: BBa_I714075
start: 1
end: 94
orientation: inline

BBa_I13516
type: ComponentDefinition
persistent identity: BBa I13516
display id: BBa_I13516
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid Intermediate
creator: jkm
created: 2005-06-05T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
mutable notes: Deletion of the C at position 1194. This is right at the end of I0500, after the transcriptional start of the downstream gene but before the RBS. Shouldn't make a difference, but needs to be checked out.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E1010
component: BBa_B0034
component: BBa_I0500
sequence annotation: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence: https://synbiohub.org/public/igem/BBa_I13516_sequence/1

BBa_E1010
type: Component
persistent identity: component1505497
display id: component1505497
version: 1
top level: BBa_I13516
access: public
definition: mRFP1

BBa_B0034
type: Component
persistent identity: component1505490
display id: component1505490
version: 1
top level: BBa_I13516
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component1505485
display id: component1505485
version: 1
top level: BBa_I13516
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505497
display id: annotation1505497
version: 1
top level: BBa_I13516
location: https://synbiohub.org/public/igem/BBa_I13516/annotation1505497/range1505497/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13516/annotation1505497/range1505497/1
type: Range
persistent identity: range1505497
display id: range1505497
version: 1
direction: direction/forward
top level: BBa_I13516
start: 1237
end: 1917
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505490
display id: annotation1505490
version: 1
top level: BBa_I13516
location: https://synbiohub.org/public/igem/BBa_I13516/annotation1505490/range1505490/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13516/annotation1505490/range1505490/1
type: Range
persistent identity: range1505490
display id: range1505490
version: 1
direction: direction/forward
top level: BBa_I13516
start: 1219
end: 1230
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1505485
display id: annotation1505485
version: 1
top level: BBa_I13516
location: https://synbiohub.org/public/igem/BBa_I13516/annotation1505485/range1505485/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13516/annotation1505485/range1505485/1
type: Range
persistent identity: range1505485
display id: range1505485
version: 1
direction: direction/forward
top level: BBa_I13516
start: 1
end: 1210
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06702
display id: BBa_J06702
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mCherry, bacterial with RBS and forward terminator
creator: Yves Wang
created: 2005-07-24T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Combines BBa_B0015 forward terminator with BBa_J06602 mCherry, bacterial with RBS
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_J06504
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J06504
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J06702_sequence/1

BBa_B0010
type: Component
persistent identity: component1596432
display id: component1596432
version: 1
top level: mCherry
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1596442
display id: component1596442
version: 1
top level: mCherry
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J06504
type: Component
persistent identity: component1596427
display id: component1596427
version: 1
top level: mCherry
access: public
definition: https://synbiohub.org/public/igem/BBa_J06504/1

BBa_B0034
type: Component
persistent identity: component1596419
display id: component1596419
version: 1
top level: mCherry
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1596432
display id: annotation1596432
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06702/annotation1596432/range1596432/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J06702/annotation1596432/range1596432/1
type: Range
persistent identity: range1596432
display id: range1596432
version: 1
direction: direction/forward
top level: mCherry
start: 741
end: 820
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1596442
display id: annotation1596442
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06702/annotation1596442/range1596442/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J06702/annotation1596442/range1596442/1
type: Range
persistent identity: range1596442
display id: range1596442
version: 1
direction: direction/forward
top level: mCherry
start: 829
end: 869
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation1596427
display id: annotation1596427
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06702/annotation1596427/range1596427/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_J06702/annotation1596427/range1596427/1
type: Range
persistent identity: range1596427
display id: range1596427
version: 1
direction: direction/forward
top level: mCherry
start: 19
end: 732
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1596419
display id: annotation1596419
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06702/annotation1596419/range1596419/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J06702/annotation1596419/range1596419/1
type: Range
persistent identity: range1596419
display id: range1596419
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 12
orientation: inline

BBa_I13405
type: ComponentDefinition
persistent identity: BBa I13405
display id: BBa_I13405
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.YFP + Pbad CFP
creator: jkm
created: 2005-04-26T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _6_
m user id: 0
owner id: 2
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Part for screening plasmid test (to be combined with an upstream inverter library).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0030
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_E0020
component: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13405_sequence/1

BBa_E0030
type: Component
persistent identity: component1480605
display id: component1480605
version: 1
top level: BBa_I13405
access: public
definition: eyfp

BBa_B0034
type: Component
persistent identity: component1480630
display id: component1480630
version: 1
top level: BBa_I13405
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1480610
display id: component1480610
version: 1
top level: BBa_I13405
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1480603
display id: component1480603
version: 1
top level: BBa_I13405
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1480620
display id: component1480620
version: 1
top level: BBa_I13405
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1480647
display id: component1480647
version: 1
top level: BBa_I13405
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1480637
display id: component1480637
version: 1
top level: BBa_I13405
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0020
type: Component
persistent identity: component1480632
display id: component1480632
version: 1
top level: BBa_I13405
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_I0500
type: Component
persistent identity: component1480625
display id: component1480625
version: 1
top level: BBa_I13405
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1480620
display id: annotation1480620
version: 1
top level: BBa_I13405
location: https://synbiohub.org/public/igem/BBa_I13405/annotation1480620/range1480620/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13405/annotation1480620/range1480620/1
type: Range
persistent identity: range1480620
display id: range1480620
version: 1
direction: direction/forward
top level: BBa_I13405
start: 838
end: 878
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1480647
display id: annotation1480647
version: 1
top level: BBa_I13405
location: https://synbiohub.org/public/igem/BBa_I13405/annotation1480647/range1480647/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13405/annotation1480647/range1480647/1
type: Range
persistent identity: range1480647
display id: range1480647
version: 1
direction: direction/forward
top level: BBa_I13405
start: 2942
end: 2982
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1480637
display id: annotation1480637
version: 1
top level: BBa_I13405
location: https://synbiohub.org/public/igem/BBa_I13405/annotation1480637/range1480637/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13405/annotation1480637/range1480637/1
type: Range
persistent identity: range1480637
display id: range1480637
version: 1
direction: direction/forward
top level: BBa_I13405
start: 2854
end: 2933
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1480610
display id: annotation1480610
version: 1
top level: BBa_I13405
location: https://synbiohub.org/public/igem/BBa_I13405/annotation1480610/range1480610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13405/annotation1480610/range1480610/1
type: Range
persistent identity: range1480610
display id: range1480610
version: 1
direction: direction/forward
top level: BBa_I13405
start: 750
end: 829
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1480625
display id: annotation1480625
version: 1
top level: BBa_I13405
location: https://synbiohub.org/public/igem/BBa_I13405/annotation1480625/range1480625/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13405/annotation1480625/range1480625/1
type: Range
persistent identity: range1480625
display id: range1480625
version: 1
direction: direction/forward
top level: BBa_I13405
start: 887
end: 2096
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1480630
display id: annotation1480630
version: 1
top level: BBa_I13405
location: https://synbiohub.org/public/igem/BBa_I13405/annotation1480630/range1480630/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13405/annotation1480630/range1480630/1
type: Range
persistent identity: range1480630
display id: range1480630
version: 1
direction: direction/forward
top level: BBa_I13405
start: 2105
end: 2116
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1480632
display id: annotation1480632
version: 1
top level: BBa_I13405
location: https://synbiohub.org/public/igem/BBa_I13405/annotation1480632/range1480632/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I13405/annotation1480632/range1480632/1
type: Range
persistent identity: range1480632
display id: range1480632
version: 1
direction: direction/forward
top level: BBa_I13405
start: 2123
end: 2845
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1480603
display id: annotation1480603
version: 1
top level: BBa_I13405
location: https://synbiohub.org/public/igem/BBa_I13405/annotation1480603/range1480603/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13405/annotation1480603/range1480603/1
type: Range
persistent identity: range1480603
display id: range1480603
version: 1
direction: direction/forward
top level: BBa_I13405
start: 1
end: 12
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1480605
display id: annotation1480605
version: 1
top level: BBa_I13405
location: https://synbiohub.org/public/igem/BBa_I13405/annotation1480605/range1480605/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13405/annotation1480605/range1480605/1
type: Range
persistent identity: range1480605
display id: range1480605
version: 1
direction: direction/forward
top level: BBa_I13405
start: 19
end: 741
orientation: inline

BBa_S03881
type: ComponentDefinition
persistent identity: BBa S03881
display id: BBa_S03881
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I14016:S03873
creator: Kathy Lam
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:32Z
discontinued: false
dominant: false
group u list: _82_
m user id: 0
owner id: 927
owning group id: 82
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I14016
component: BBa_S03873
sequence annotation: BBa_S03873
sequence annotation: BBa_I14016
sequence: https://synbiohub.org/public/igem/BBa_S03881_sequence/1

BBa_I14016
type: Component
persistent identity: component2245775
display id: component2245775
version: 1
top level: BBa_S03881
access: public
definition: https://synbiohub.org/public/igem/BBa_I14016/1

BBa_S03873
type: Component
persistent identity: component2245781
display id: component2245781
version: 1
top level: BBa_S03881
access: public
definition: https://synbiohub.org/public/igem/BBa_S03873/1

BBa_S03873
type: SequenceAnnotation
persistent identity: annotation2245781
display id: annotation2245781
version: 1
top level: BBa_S03881
location: https://synbiohub.org/public/igem/BBa_S03881/annotation2245781/range2245781/1
component: BBa_S03873

https://synbiohub.org/public/igem/BBa_S03881/annotation2245781/range2245781/1
type: Range
persistent identity: range2245781
display id: range2245781
version: 1
direction: direction/reverse
top level: BBa_S03881
start: 177
end: 900
orientation: inline

BBa_I14016
type: SequenceAnnotation
persistent identity: annotation2245775
display id: annotation2245775
version: 1
top level: BBa_S03881
location: https://synbiohub.org/public/igem/BBa_S03881/annotation2245775/range2245775/1
component: BBa_I14016

https://synbiohub.org/public/igem/BBa_S03881/annotation2245775/range2245775/1
type: Range
persistent identity: range2245775
display id: range2245775
version: 1
direction: direction/reverse
top level: BBa_S03881
start: 1
end: 168
orientation: inline

BBa_I13538
type: ComponentDefinition
persistent identity: BBa I13538
display id: BBa_I13538
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Two promoter screening plasmid v1.1
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E1010
component: BBa_B0012
component: BBa_B0010
component: BBa_I13457
component: BBa_I13453
component: BBa_E0040
component: BBa_I0500
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I13453
sequence annotation: BBa_E0040
sequence annotation: BBa_I13457
sequence: https://synbiohub.org/public/igem/BBa_I13538_sequence/1

BBa_B0034
type: Component
persistent identity: component1663699
display id: component1663699
version: 1
top level: BBa_I13538
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: Component
persistent identity: component1663706
display id: component1663706
version: 1
top level: BBa_I13538
access: public
definition: mRFP1

BBa_B0012
type: Component
persistent identity: component1663722
display id: component1663722
version: 1
top level: BBa_I13538
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1663659
display id: component1663659
version: 1
top level: BBa_I13538
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13457
type: Component
persistent identity: component1663694
display id: component1663694
version: 1
top level: BBa_I13538
access: public
definition: https://synbiohub.org/public/igem/BBa_I13457/1

BBa_B0010
type: Component
persistent identity: component1663712
display id: component1663712
version: 1
top level: BBa_I13538
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13453
type: Component
persistent identity: component1663674
display id: component1663674
version: 1
top level: BBa_I13538
access: public
definition: BBa_I13453

BBa_E0040
type: Component
persistent identity: component1663654
display id: component1663654
version: 1
top level: BBa_I13538
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1663669
display id: component1663669
version: 1
top level: BBa_I13538
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1663652
display id: component1663652
version: 1
top level: BBa_I13538
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component1663647
display id: component1663647
version: 1
top level: BBa_I13538
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663699
display id: annotation1663699
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663699/range1663699/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13538/annotation1663699/range1663699/1
type: Range
persistent identity: range1663699
display id: range1663699
version: 1
direction: direction/forward
top level: BBa_I13538
start: 2359
end: 2370
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1663647
display id: annotation1663647
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663647/range1663647/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13538/annotation1663647/range1663647/1
type: Range
persistent identity: range1663647
display id: range1663647
version: 1
direction: direction/forward
top level: BBa_I13538
start: 1
end: 1210
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1663706
display id: annotation1663706
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663706/range1663706/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13538/annotation1663706/range1663706/1
type: Range
persistent identity: range1663706
display id: range1663706
version: 1
direction: direction/forward
top level: BBa_I13538
start: 2377
end: 3057
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1663722
display id: annotation1663722
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663722/range1663722/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13538/annotation1663722/range1663722/1
type: Range
persistent identity: range1663722
display id: range1663722
version: 1
direction: direction/forward
top level: BBa_I13538
start: 3179
end: 3219
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663712
display id: annotation1663712
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663712/range1663712/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13538/annotation1663712/range1663712/1
type: Range
persistent identity: range1663712
display id: range1663712
version: 1
direction: direction/forward
top level: BBa_I13538
start: 3091
end: 3170
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1663659
display id: annotation1663659
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663659/range1663659/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13538/annotation1663659/range1663659/1
type: Range
persistent identity: range1663659
display id: range1663659
version: 1
direction: direction/forward
top level: BBa_I13538
start: 1965
end: 2044
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1663674
display id: annotation1663674
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663674/range1663674/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13538/annotation1663674/range1663674/1
type: Range
persistent identity: range1663674
display id: range1663674
version: 1
direction: direction/forward
top level: BBa_I13538
start: 2102
end: 2231
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1663654
display id: annotation1663654
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663654/range1663654/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13538/annotation1663654/range1663654/1
type: Range
persistent identity: range1663654
display id: range1663654
version: 1
direction: direction/forward
top level: BBa_I13538
start: 1237
end: 1956
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1663652
display id: annotation1663652
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663652/range1663652/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13538/annotation1663652/range1663652/1
type: Range
persistent identity: range1663652
display id: range1663652
version: 1
direction: direction/forward
top level: BBa_I13538
start: 1219
end: 1230
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1663669
display id: annotation1663669
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663669/range1663669/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13538/annotation1663669/range1663669/1
type: Range
persistent identity: range1663669
display id: range1663669
version: 1
direction: direction/forward
top level: BBa_I13538
start: 2053
end: 2093
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation1663694
display id: annotation1663694
version: 1
top level: BBa_I13538
location: https://synbiohub.org/public/igem/BBa_I13538/annotation1663694/range1663694/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13538/annotation1663694/range1663694/1
type: Range
persistent identity: range1663694
display id: range1663694
version: 1
direction: direction/forward
top level: BBa_I13538
start: 2240
end: 2350
orientation: inline

BBa_I732807
type: ComponentDefinition
persistent identity: BBa I732807
display id: BBa_I732807
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0103 (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_I732107
sequence annotation: BBa_I732107
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732807_sequence/1

BBa_B0034
type: Component
persistent identity: component1946183
display id: component1946183
version: 1
top level: BBa_I732807
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732107
type: Component
persistent identity: component1946184
display id: component1946184
version: 1
top level: BBa_I732807
access: public
definition: ARL2A0103

BBa_I732107
type: SequenceAnnotation
persistent identity: annotation1946184
display id: annotation1946184
version: 1
top level: BBa_I732807
location: https://synbiohub.org/public/igem/BBa_I732807/annotation1946184/range1946184/1
component: BBa_I732107

https://synbiohub.org/public/igem/BBa_I732807/annotation1946184/range1946184/1
type: Range
persistent identity: range1946184
display id: range1946184
version: 1
direction: direction/reverse
top level: BBa_I732807
start: 19
end: 1104
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946183
display id: annotation1946183
version: 1
top level: BBa_I732807
location: https://synbiohub.org/public/igem/BBa_I732807/annotation1946183/range1946183/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732807/annotation1946183/range1946183/1
type: Range
persistent identity: range1946183
display id: range1946183
version: 1
direction: direction/reverse
top level: BBa_I732807
start: 1
end: 12
orientation: inline

lasr
type: ComponentDefinition
persistent identity: BBa C0079
display id: BBa_C0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasR activator from P. aeruginosa PAO1(+LVA)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein)
mutable notes: Mutated 480 from g to a to remove PstI site
mutable provenance: Genbank: NC_002516 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lasR
sequence annotation: G
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0079_sequence/1

lasR
type: SequenceAnnotation
persistent identity: annotation307935
display id: annotation307935
version: 1
top level: lasr
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1

https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
type: Range
persistent identity: range307935
display id: range307935
version: 1
direction: direction/forward
top level: lasr
start: 1
end: 717
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308006
display id: annotation308006
version: 1
top level: lasr
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1

https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
type: Range
persistent identity: range308006
display id: range308006
version: 1
direction: direction/forward
top level: lasr
start: 480
end: 480
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213999
display id: annotation2213999
version: 1
top level: lasr
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1

https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
type: Range
persistent identity: range2213999
display id: range2213999
version: 1
direction: direction/forward
top level: lasr
start: 757
end: 781
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation307948
display id: annotation307948
version: 1
top level: lasr
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1

https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
type: Range
persistent identity: range307948
display id: range307948
version: 1
direction: direction/forward
top level: lasr
start: 718
end: 750
orientation: inline

BBa_K118003
type: ComponentDefinition
persistent identity: BBa K118003
display id: BBa_K118003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crtI coding sequence encoding phytoene dehydrogenase
creator: Andrew Hall
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PstI site removed
sequence annotation: crtI coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118003_sequence/1

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968244
display id: annotation1968244
version: 1
top level: BBa_K118003
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1

https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
type: Range
persistent identity: range1968244
display id: range1968244
version: 1
direction: direction/forward
top level: BBa_K118003
start: 478
end: 483
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968245
display id: annotation1968245
version: 1
top level: BBa_K118003
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1

https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
type: Range
persistent identity: range1968245
display id: range1968245
version: 1
direction: direction/forward
top level: BBa_K118003
start: 889
end: 894
orientation: inline

crtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1995702
display id: annotation1995702
version: 1
top level: BBa_K118003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1

https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
type: Range
persistent identity: range1995702
display id: range1995702
version: 1
direction: direction/forward
top level: BBa_K118003
start: 1
end: 1479
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0160
display id: BBa_C0160
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer inactivation enzyme aiiA (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0060 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: aiiA
sequence: https://synbiohub.org/public/igem/BBa_C0160_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1891585
display id: annotation1891585
version: 1
top level: aiiA
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1

https://synbiohub.org/public/igem/BBa_C0160/annotation1891585/range1891585/1
type: Range
persistent identity: range1891585
display id: range1891585
version: 1
direction: direction/forward
top level: aiiA
start: 1
end: 756
orientation: inline

BBa_I746105
type: ComponentDefinition
persistent identity: BBa I746105
display id: BBa_I746105
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP with agr P2 promoter
creator: Zhizhen Zhao
created: 2007-09-06T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2121
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is composed of a promoter and a GFP reporter gene. This can produce green fluorescence and in theory the strength of the green flourence is much stronger when there is phosphorylated AgrA in the cell. This is used to test the basal activity of agr P2 promoter.
mutable notes: NA
mutable provenance: We used two subparts from the registry to produce the new part. BBa_I746104+BBa_E0840
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_I746104
component: BBa_B0030
component: BBa_E0040
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_B0010
sequence annotation: BBa_I746104
sequence: https://synbiohub.org/public/igem/BBa_I746105_sequence/1

BBa_B0012
type: Component
persistent identity: component1943717
display id: component1943717
version: 1
top level: BBa_I746105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1943715
display id: component1943715
version: 1
top level: BBa_I746105
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746104
type: Component
persistent identity: component1943709
display id: component1943709
version: 1
top level: BBa_I746105
access: public
definition: BBa_I746104

BBa_B0030
type: Component
persistent identity: component1943711
display id: component1943711
version: 1
top level: BBa_I746105
access: public
definition: BBa_B0030

BBa_E0040
type: Component
persistent identity: component1943714
display id: component1943714
version: 1
top level: BBa_I746105
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1943714
display id: annotation1943714
version: 1
top level: BBa_I746105
location: https://synbiohub.org/public/igem/BBa_I746105/annotation1943714/range1943714/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I746105/annotation1943714/range1943714/1
type: Range
persistent identity: range1943714
display id: range1943714
version: 1
direction: direction/reverse
top level: BBa_I746105
start: 126
end: 845
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1943717
display id: annotation1943717
version: 1
top level: BBa_I746105
location: https://synbiohub.org/public/igem/BBa_I746105/annotation1943717/range1943717/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746105/annotation1943717/range1943717/1
type: Range
persistent identity: range1943717
display id: range1943717
version: 1
direction: direction/forward
top level: BBa_I746105
start: 942
end: 982
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1943711
display id: annotation1943711
version: 1
top level: BBa_I746105
location: https://synbiohub.org/public/igem/BBa_I746105/annotation1943711/range1943711/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I746105/annotation1943711/range1943711/1
type: Range
persistent identity: range1943711
display id: range1943711
version: 1
direction: direction/forward
top level: BBa_I746105
start: 105
end: 119
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1943715
display id: annotation1943715
version: 1
top level: BBa_I746105
location: https://synbiohub.org/public/igem/BBa_I746105/annotation1943715/range1943715/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746105/annotation1943715/range1943715/1
type: Range
persistent identity: range1943715
display id: range1943715
version: 1
direction: direction/forward
top level: BBa_I746105
start: 854
end: 933
orientation: inline

BBa_I746104
type: SequenceAnnotation
persistent identity: annotation1943709
display id: annotation1943709
version: 1
top level: BBa_I746105
location: https://synbiohub.org/public/igem/BBa_I746105/annotation1943709/range1943709/1
component: BBa_I746104

https://synbiohub.org/public/igem/BBa_I746105/annotation1943709/range1943709/1
type: Range
persistent identity: range1943709
display id: range1943709
version: 1
direction: direction/reverse
top level: BBa_I746105
start: 1
end: 96
orientation: inline

BBa_A340620
type: ComponentDefinition
persistent identity: BBa A340620
display id: BBa_A340620
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Quadpart Amplifier (B0034.C0062.B0015.R0062)
creator: cconboy
created: 2004-08-09T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_R0062
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_C0062
sequence annotation: BBa_C0062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_A340620_sequence/1

BBa_R0062
type: Component
persistent identity: component1041235
display id: component1041235
version: 1
top level: BBa_A340620
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0034
type: Component
persistent identity: component1041187
display id: component1041187
version: 1
top level: BBa_A340620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1041220
display id: component1041220
version: 1
top level: BBa_A340620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1041210
display id: component1041210
version: 1
top level: BBa_A340620
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0062
type: Component
persistent identity: component1041202
display id: component1041202
version: 1
top level: BBa_A340620
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1041202
display id: annotation1041202
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041202/range1041202/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_A340620/annotation1041202/range1041202/1
type: Range
persistent identity: range1041202
display id: range1041202
version: 1
direction: direction/forward
top level: BBa_A340620
start: 19
end: 774
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1041220
display id: annotation1041220
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041220/range1041220/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_A340620/annotation1041220/range1041220/1
type: Range
persistent identity: range1041220
display id: range1041220
version: 1
direction: direction/forward
top level: BBa_A340620
start: 896
end: 936
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1041210
display id: annotation1041210
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041210/range1041210/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_A340620/annotation1041210/range1041210/1
type: Range
persistent identity: range1041210
display id: range1041210
version: 1
direction: direction/forward
top level: BBa_A340620
start: 808
end: 887
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1041235
display id: annotation1041235
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041235/range1041235/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_A340620/annotation1041235/range1041235/1
type: Range
persistent identity: range1041235
display id: range1041235
version: 1
direction: direction/forward
top level: BBa_A340620
start: 945
end: 999
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1041187
display id: annotation1041187
version: 1
top level: BBa_A340620
location: https://synbiohub.org/public/igem/BBa_A340620/annotation1041187/range1041187/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_A340620/annotation1041187/range1041187/1
type: Range
persistent identity: range1041187
display id: range1041187
version: 1
direction: direction/forward
top level: BBa_A340620
start: 1
end: 12
orientation: inline

BBa_S03858
type: ComponentDefinition
persistent identity: BBa S03858
display id: BBa_S03858
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I718017:B0015
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 1568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0010
component: BBa_I718017
component: BBa_B0012
sequence annotation: BBa_I718017
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S03858_sequence/1

BBa_B0010
type: Component
persistent identity: component1956462
display id: component1956462
version: 1
top level: BBa_S03858
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I718017
type: Component
persistent identity: component1956461
display id: component1956461
version: 1
top level: BBa_S03858
access: public
definition: lox71

BBa_B0012
type: Component
persistent identity: component1956464
display id: component1956464
version: 1
top level: BBa_S03858
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I718017
type: SequenceAnnotation
persistent identity: annotation1956461
display id: annotation1956461
version: 1
top level: BBa_S03858
location: https://synbiohub.org/public/igem/BBa_S03858/annotation1956461/range1956461/1
component: BBa_I718017

https://synbiohub.org/public/igem/BBa_S03858/annotation1956461/range1956461/1
type: Range
persistent identity: range1956461
display id: range1956461
version: 1
direction: direction/reverse
top level: BBa_S03858
start: 1
end: 34
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956464
display id: annotation1956464
version: 1
top level: BBa_S03858
location: https://synbiohub.org/public/igem/BBa_S03858/annotation1956464/range1956464/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03858/annotation1956464/range1956464/1
type: Range
persistent identity: range1956464
display id: range1956464
version: 1
direction: direction/forward
top level: BBa_S03858
start: 131
end: 171
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956462
display id: annotation1956462
version: 1
top level: BBa_S03858
location: https://synbiohub.org/public/igem/BBa_S03858/annotation1956462/range1956462/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03858/annotation1956462/range1956462/1
type: Range
persistent identity: range1956462
display id: range1956462
version: 1
direction: direction/forward
top level: BBa_S03858
start: 43
end: 122
orientation: inline

BBa_E0432
type: ComponentDefinition
persistent identity: BBa E0432
display id: BBa_E0432
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: EYFP (RBS+ LVA+ TERM) (B0034.E0032.B0015)
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_E0032
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0432_sequence/1

BBa_B0012
type: Component
persistent identity: component942938
display id: component942938
version: 1
top level: BBa_E0432
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0032
type: Component
persistent identity: component942920
display id: component942920
version: 1
top level: BBa_E0432
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0010
type: Component
persistent identity: component942928
display id: component942928
version: 1
top level: BBa_E0432
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component942908
display id: component942908
version: 1
top level: BBa_E0432
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation942928
display id: annotation942928
version: 1
top level: BBa_E0432
location: https://synbiohub.org/public/igem/BBa_E0432/annotation942928/range942928/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0432/annotation942928/range942928/1
type: Range
persistent identity: range942928
display id: range942928
version: 1
direction: direction/forward
top level: BBa_E0432
start: 789
end: 868
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation942908
display id: annotation942908
version: 1
top level: BBa_E0432
location: https://synbiohub.org/public/igem/BBa_E0432/annotation942908/range942908/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0432/annotation942908/range942908/1
type: Range
persistent identity: range942908
display id: range942908
version: 1
direction: direction/forward
top level: BBa_E0432
start: 1
end: 12
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation942920
display id: annotation942920
version: 1
top level: BBa_E0432
location: https://synbiohub.org/public/igem/BBa_E0432/annotation942920/range942920/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_E0432/annotation942920/range942920/1
type: Range
persistent identity: range942920
display id: range942920
version: 1
direction: direction/forward
top level: BBa_E0432
start: 19
end: 780
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation942938
display id: annotation942938
version: 1
top level: BBa_E0432
location: https://synbiohub.org/public/igem/BBa_E0432/annotation942938/range942938/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0432/annotation942938/range942938/1
type: Range
persistent identity: range942938
display id: range942938
version: 1
direction: direction/forward
top level: BBa_E0432
start: 877
end: 917
orientation: inline

ARL2A0102
type: ComponentDefinition
persistent identity: BBa I732106
display id: BBa_I732106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0102
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732106_sequence/1

spacer
type: ComponentDefinition
persistent identity: BBa B0040
display id: BBa_B0040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Spacer.1 (generic)
creator: Vinay S. Mahajan, Brian Chow, Peter Carr
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Generic spacer for ensuring a 70 bp distance between the end of the suffix of the BioBrick part containing the double terminator and the prefix of the BioBrick part containing the promoter of the new gene. Please, use the AlignX function of Vector NT to check for homology with the components in your plasmid before using this spacer.
mutable notes: The size of the spacer was choosed to meet the minimum length of a sequence that can be queried using the BLAST search engine. However, subsequences of it can be used to design shorter spacers. The sequence was selected from many more sequences randomly generated using the Random DNA Generator engine; the GC% parameter used as input was 50%. The sequences were selected based on the following constraints listed in their order of importance: the absence of any putative promoter regions, a low degree of homology with the Elowitz plasmid (whose components are widely used in our designs), no homology with other E.coli sequences as shown by BLASTN search results and the presence of a number of TAA stop codons. The second constraint was the most stringent leading to the elimination of most sequences. DE made the following changes to the original sequence in order to add stop codons in the -3 frame and more in the +2 frame (note, not all of these stop codons are UAA. Thus, if used in an organism that inserts an amino acid @ UGA or UAG the obvious will occur): T->A @ 85 T->A @ 42 C->T @ 79 A->T @ 64 A->T @ 31 T->A @ 34 C->A @ 37 Also, note that the above changes further reduce (the already very weak) homology to current NCBI-stored sequences. In the process of selecting the best sequence it appeared that a good alternative sequence for a spacer would be: AGGTTCTGATATGTAACTGTGCCCAATGTCGTTAGTGACGCATACCTCTTAAGAGGCCACTGTCCTAACA. The sequence contains no putative promoters and shows moderate homology with the 5' end of the Ampicillin resistance gene. However a strong promoter sequence starts 12 bp downstream of this sequence, and therefore the sequence presented above was preferred. The sequence is compatible (does not show significant homology) with the components in the Elowitz repressilator plasmid.
mutable provenance: Randomly generated and optimized for several parameters (see Design notes).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Spacer-1
sequence annotation: BBa_B0040
sequence: https://synbiohub.org/public/igem/BBa_B0040_sequence/1

Spacer-1
type: SequenceAnnotation
persistent identity: annotation1721
display id: annotation1721
version: 1
top level: spacer
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1

https://synbiohub.org/public/igem/BBa_B0040/annotation1721/range1721/1
type: Range
persistent identity: range1721
display id: range1721
version: 1
direction: direction/forward
top level: spacer
start: 1
end: 70
orientation: inline

BBa_B0040
type: SequenceAnnotation
persistent identity: annotation7030
display id: annotation7030
version: 1
top level: spacer
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1

https://synbiohub.org/public/igem/BBa_B0040/annotation7030/range7030/1
type: Range
persistent identity: range7030
display id: range7030
version: 1
direction: direction/forward
top level: spacer
start: 1
end: 70
orientation: inline

BBa_J04971
type: ComponentDefinition
persistent identity: BBa J04971
display id: BBa_J04971
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BBa_J04900 (3AptRibo) Riboswitch with BBa_J04671 (EYFP, RBS-, LVA+, B0015)
creator: Andrew Drysdale and Kristen DeCelle (also Oscar Hernandez and Matt Gemberling)
created: 2005-11-02T12:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _16_
m user id: 0
owner id: 435
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a (3AptRibo) Riboswitch device that contains a EYFP reporter region with an LVA tag and a B0015 Terminator Sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_E0032
component: BBa_B0012
component: BBa_B0010
component: BBa_J04900
sequence annotation: BBa_B0012
sequence annotation: BBa_J04900
sequence annotation: BBa_B0010
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_J04971_sequence/1

BBa_E0032
type: Component
persistent identity: component1735335
display id: component1735335
version: 1
top level: BBa_J04971
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0012
type: Component
persistent identity: component1735353
display id: component1735353
version: 1
top level: BBa_J04971
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1735343
display id: component1735343
version: 1
top level: BBa_J04971
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J04900
type: Component
persistent identity: component1735323
display id: component1735323
version: 1
top level: BBa_J04971
access: public
definition: https://synbiohub.org/public/igem/BBa_J04900/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1735353
display id: annotation1735353
version: 1
top level: BBa_J04971
location: https://synbiohub.org/public/igem/BBa_J04971/annotation1735353/range1735353/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04971/annotation1735353/range1735353/1
type: Range
persistent identity: range1735353
display id: range1735353
version: 1
direction: direction/forward
top level: BBa_J04971
start: 1123
end: 1163
orientation: inline

BBa_J04900
type: SequenceAnnotation
persistent identity: annotation1735323
display id: annotation1735323
version: 1
top level: BBa_J04971
location: https://synbiohub.org/public/igem/BBa_J04971/annotation1735323/range1735323/1
component: BBa_J04900

https://synbiohub.org/public/igem/BBa_J04971/annotation1735323/range1735323/1
type: Range
persistent identity: range1735323
display id: range1735323
version: 1
direction: direction/forward
top level: BBa_J04971
start: 1
end: 258
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1735343
display id: annotation1735343
version: 1
top level: BBa_J04971
location: https://synbiohub.org/public/igem/BBa_J04971/annotation1735343/range1735343/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04971/annotation1735343/range1735343/1
type: Range
persistent identity: range1735343
display id: range1735343
version: 1
direction: direction/forward
top level: BBa_J04971
start: 1035
end: 1114
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1735335
display id: annotation1735335
version: 1
top level: BBa_J04971
location: https://synbiohub.org/public/igem/BBa_J04971/annotation1735335/range1735335/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_J04971/annotation1735335/range1735335/1
type: Range
persistent identity: range1735335
display id: range1735335
version: 1
direction: direction/forward
top level: BBa_J04971
start: 265
end: 1026
orientation: inline

BBa_I746104
type: ComponentDefinition
persistent identity: BBa I746104
display id: BBa_I746104
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P2 promoter in agr operon from S. aureus
creator: Zhizhen Zhao
created: 2007-08-30T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2121
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the expression level from P2 promoter.
mutable notes: Codon usage has been optimised for E. coli (which also seems to give good results for B. subtilis
mutable provenance: The section of the sequence constituting the P2 promoter is subject to rather more debate than the sequences of the coding regions. [http://jb.asm.org/cgi/content/full/186/22/7549 Koenig, Robbin L., Ray, Jessica L., Maleki, Soheila J., Smeltzer, Mark S., Hurlburt, Barry K. Staphylococcus aureus AgrA Binding to the RNAIII-agr Regulatory Region J. Bacteriol. 2004 186: 7549-7555] shows the areas of the sequence to which phosphorylated AgrA binds while L Rao, R K Karls, and M J Betley: In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus. Bacteriol. 1995 May; 177(10): 2609???2614. shows the transcription start sites; we took the overall extent of the promoter region from that labelled in Morfeldt, E., K. Tegmark, and S. Arvidson. 1996. Transcriptional control of the agr-dependent virulence gene regulator, RNAIII, in Staphylococcus aureus. Mol. Microbiol. 21:1227???1237. (not available online).
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I746104_sequence/1

BBa_S03857
type: ComponentDefinition
persistent identity: BBa S03857
display id: BBa_S03857
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E0840:I718016
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 1568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I718016
component: BBa_B0030
component: BBa_B0012
component: BBa_E0040
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_I718016
sequence: https://synbiohub.org/public/igem/BBa_S03857_sequence/1

BBa_I718016
type: Component
persistent identity: component1956459
display id: component1956459
version: 1
top level: BBa_S03857
access: public
definition: https://synbiohub.org/public/igem/BBa_I718016/1

BBa_B0030
type: Component
persistent identity: component1956449
display id: component1956449
version: 1
top level: BBa_S03857
access: public
definition: BBa_B0030

BBa_B0012
type: Component
persistent identity: component1956455
display id: component1956455
version: 1
top level: BBa_S03857
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1956452
display id: component1956452
version: 1
top level: BBa_S03857
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1956453
display id: component1956453
version: 1
top level: BBa_S03857
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956453
display id: annotation1956453
version: 1
top level: BBa_S03857
location: https://synbiohub.org/public/igem/BBa_S03857/annotation1956453/range1956453/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03857/annotation1956453/range1956453/1
type: Range
persistent identity: range1956453
display id: range1956453
version: 1
direction: direction/forward
top level: BBa_S03857
start: 750
end: 829
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1956452
display id: annotation1956452
version: 1
top level: BBa_S03857
location: https://synbiohub.org/public/igem/BBa_S03857/annotation1956452/range1956452/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S03857/annotation1956452/range1956452/1
type: Range
persistent identity: range1956452
display id: range1956452
version: 1
direction: direction/reverse
top level: BBa_S03857
start: 22
end: 741
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956449
display id: annotation1956449
version: 1
top level: BBa_S03857
location: https://synbiohub.org/public/igem/BBa_S03857/annotation1956449/range1956449/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_S03857/annotation1956449/range1956449/1
type: Range
persistent identity: range1956449
display id: range1956449
version: 1
direction: direction/forward
top level: BBa_S03857
start: 1
end: 15
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956455
display id: annotation1956455
version: 1
top level: BBa_S03857
location: https://synbiohub.org/public/igem/BBa_S03857/annotation1956455/range1956455/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03857/annotation1956455/range1956455/1
type: Range
persistent identity: range1956455
display id: range1956455
version: 1
direction: direction/forward
top level: BBa_S03857
start: 838
end: 878
orientation: inline

BBa_I718016
type: SequenceAnnotation
persistent identity: annotation1956459
display id: annotation1956459
version: 1
top level: BBa_S03857
location: https://synbiohub.org/public/igem/BBa_S03857/annotation1956459/range1956459/1
component: BBa_I718016

https://synbiohub.org/public/igem/BBa_S03857/annotation1956459/range1956459/1
type: Range
persistent identity: range1956459
display id: range1956459
version: 1
direction: direction/reverse
top level: BBa_S03857
start: 887
end: 920
orientation: inline

BBa_I13261
type: ComponentDefinition
persistent identity: BBa I13261
display id: BBa_I13261
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lux Receiver (I13263 with reversed part order)
creator: Barry Canton
created: 2004-10-30T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Receiver circuit similar to I13263 but with the order of the reporter and luxR generator reversed
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0062
component: BBa_R0063
component: BBa_C0062
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_C0062
sequence annotation: BBa_E0030
sequence annotation: BBa_R0063
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_I13261_sequence/1

BBa_R0062
type: Component
persistent identity: component1250950
display id: component1250950
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_R0063
type: Component
persistent identity: component1250992
display id: component1250992
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_C0062
type: Component
persistent identity: component1251016
display id: component1251016
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0010
type: Component
persistent identity: component1251024
display id: component1251024
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1251034
display id: component1251034
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1251001
display id: component1251001
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1250965
display id: component1250965
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1250975
display id: component1250975
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1250958
display id: component1250958
version: 1
top level: BBa_I13261
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0030
type: Component
persistent identity: component1250960
display id: component1250960
version: 1
top level: BBa_I13261
access: public
definition: eyfp

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1250965
display id: annotation1250965
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1250965/range1250965/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13261/annotation1250965/range1250965/1
type: Range
persistent identity: range1250965
display id: range1250965
version: 1
direction: direction/forward
top level: BBa_I13261
start: 813
end: 892
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1251016
display id: annotation1251016
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1251016/range1251016/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13261/annotation1251016/range1251016/1
type: Range
persistent identity: range1251016
display id: range1251016
version: 1
direction: direction/forward
top level: BBa_I13261
start: 1127
end: 1882
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1251024
display id: annotation1251024
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1251024/range1251024/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13261/annotation1251024/range1251024/1
type: Range
persistent identity: range1251024
display id: range1251024
version: 1
direction: direction/forward
top level: BBa_I13261
start: 1916
end: 1995
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1250960
display id: annotation1250960
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1250960/range1250960/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13261/annotation1250960/range1250960/1
type: Range
persistent identity: range1250960
display id: range1250960
version: 1
direction: direction/forward
top level: BBa_I13261
start: 82
end: 804
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation1250992
display id: annotation1250992
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1250992/range1250992/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I13261/annotation1250992/range1250992/1
type: Range
persistent identity: range1250992
display id: range1250992
version: 1
direction: direction/forward
top level: BBa_I13261
start: 950
end: 1100
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1250958
display id: annotation1250958
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1250958/range1250958/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13261/annotation1250958/range1250958/1
type: Range
persistent identity: range1250958
display id: range1250958
version: 1
direction: direction/forward
top level: BBa_I13261
start: 64
end: 75
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1250975
display id: annotation1250975
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1250975/range1250975/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13261/annotation1250975/range1250975/1
type: Range
persistent identity: range1250975
display id: range1250975
version: 1
direction: direction/forward
top level: BBa_I13261
start: 901
end: 941
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1251001
display id: annotation1251001
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1251001/range1251001/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13261/annotation1251001/range1251001/1
type: Range
persistent identity: range1251001
display id: range1251001
version: 1
direction: direction/forward
top level: BBa_I13261
start: 1109
end: 1120
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1251034
display id: annotation1251034
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1251034/range1251034/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13261/annotation1251034/range1251034/1
type: Range
persistent identity: range1251034
display id: range1251034
version: 1
direction: direction/forward
top level: BBa_I13261
start: 2004
end: 2044
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1250950
display id: annotation1250950
version: 1
top level: BBa_I13261
location: https://synbiohub.org/public/igem/BBa_I13261/annotation1250950/range1250950/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13261/annotation1250950/range1250950/1
type: Range
persistent identity: range1250950
display id: range1250950
version: 1
direction: direction/forward
top level: BBa_I13261
start: 1
end: 55
orientation: inline

BBa_I13533
type: ComponentDefinition
persistent identity: BBa I13533
display id: BBa_I13533
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 2.5
creator: Josh Michener
created: 2005-07-28T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I13516
component: BBa_I13527
sequence annotation: BBa_I13527
sequence annotation: BBa_I13516
sequence: https://synbiohub.org/public/igem/BBa_I13533_sequence/1

BBa_I13516
type: Component
persistent identity: component2226159
display id: component2226159
version: 1
top level: BBa_I13533
access: public
definition: BBa_I13516

BBa_I13527
type: Component
persistent identity: component2226177
display id: component2226177
version: 1
top level: BBa_I13533
access: public
definition: https://synbiohub.org/public/igem/BBa_I13527/1

BBa_I13527
type: SequenceAnnotation
persistent identity: annotation2226177
display id: annotation2226177
version: 1
top level: BBa_I13533
location: https://synbiohub.org/public/igem/BBa_I13533/annotation2226177/range2226177/1
component: BBa_I13527

https://synbiohub.org/public/igem/BBa_I13533/annotation2226177/range2226177/1
type: Range
persistent identity: range2226177
display id: range2226177
version: 1
direction: direction/reverse
top level: BBa_I13533
start: 1951
end: 2944
orientation: inline

BBa_I13516
type: SequenceAnnotation
persistent identity: annotation2226159
display id: annotation2226159
version: 1
top level: BBa_I13533
location: https://synbiohub.org/public/igem/BBa_I13533/annotation2226159/range2226159/1
component: BBa_I13516

https://synbiohub.org/public/igem/BBa_I13533/annotation2226159/range2226159/1
type: Range
persistent identity: range2226159
display id: range2226159
version: 1
direction: direction/reverse
top level: BBa_I13533
start: 1
end: 1942
orientation: inline

rhII
type: ComponentDefinition
persistent identity: BBa C0070
display id: BBa_C0070
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Autoinducer synthesis protein that produces N-butyryl-HSL which binds to RhlR, obtained from Pseudomonas aeruginosa.
mutable notes: Editing Check (1/28/04) (0) BB sites cleaned (1) ATG (2) TAATAA (3) Blast checks out (i.e., it's RhlI) (4) Codon changes OK (AA and tRNA usage) (5) Chassis might have conflict (w/ native autoinducer system?) (6) ssrA tag added (7) barcode added (8) Codon optimized for expression in enteric bacteria 2 silent point mutations have been included into the sequence at base 138 from A to G and at base 576 from G to C to remove internal EcoRI and PstI sites. Mutations were chosen to yield the same amino acid and codons of similar frequency in E. coli.
mutable provenance: P. aeruginosa PA3476
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rhlL
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_C0070_sequence/1

rhlL
type: SequenceAnnotation
persistent identity: annotation301203
display id: annotation301203
version: 1
top level: rhII
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1

https://synbiohub.org/public/igem/BBa_C0070/annotation301203/range301203/1
type: Range
persistent identity: range301203
display id: range301203
version: 1
direction: direction/forward
top level: rhII
start: 1
end: 603
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213996
display id: annotation2213996
version: 1
top level: rhII
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1

https://synbiohub.org/public/igem/BBa_C0070/annotation2213996/range2213996/1
type: Range
persistent identity: range2213996
display id: range2213996
version: 1
direction: direction/forward
top level: rhII
start: 643
end: 667
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306820
display id: annotation306820
version: 1
top level: rhII
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1

https://synbiohub.org/public/igem/BBa_C0070/annotation306820/range306820/1
type: Range
persistent identity: range306820
display id: range306820
version: 1
direction: direction/forward
top level: rhII
start: 604
end: 636
orientation: inline

BBa_C2006
type: ComponentDefinition
persistent identity: BBa C2006
display id: BBa_C2006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: MalE.FactorXa.Zif268-GCN4
creator: Reshma Shetty
created: 2007-03-27T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Maltose binding protein fused to Zif268-GCN4.
mutable notes: None.
mutable provenance: From pMAL-c2x and BBa_C2005.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C2006_sequence/1

BBa_K145201
type: ComponentDefinition
persistent identity: BBa K145201
display id: BBa_K145201
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: INPUT TetR generator
creator: Jonas Demeulemeester
created: 2008-08-19T11:00:00Z
modified: 2015-05-08T01:10:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _257_
m user id: 0
owner id: 2970
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: INPUT TetR constitutive generator
mutable notes: chosen a medium-weak constitutive promoter. In order to have strong repression but also easy derepression by aTc.
mutable provenance: other parts
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0015
component: BBa_J23116
component: BBa_C0040
sequence annotation: BBa_C0040
sequence annotation: BBa_J23116
sequence annotation: BBa_B0034
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K145201_sequence/1

BBa_B0034
type: Component
persistent identity: component2254370
display id: component2254370
version: 1
top level: BBa_K145201
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0015
type: Component
persistent identity: component2254381
display id: component2254381
version: 1
top level: BBa_K145201
access: public
definition: BBa_B0015

BBa_J23116
type: Component
persistent identity: component2254368
display id: component2254368
version: 1
top level: BBa_K145201
access: public
definition: https://synbiohub.org/public/igem/BBa_J23116/1

BBa_C0040
type: Component
persistent identity: component2254374
display id: component2254374
version: 1
top level: BBa_K145201
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2254374
display id: annotation2254374
version: 1
top level: BBa_K145201
location: https://synbiohub.org/public/igem/BBa_K145201/annotation2254374/range2254374/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K145201/annotation2254374/range2254374/1
type: Range
persistent identity: range2254374
display id: range2254374
version: 1
direction: direction/reverse
top level: BBa_K145201
start: 62
end: 746
orientation: inline

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2254368
display id: annotation2254368
version: 1
top level: BBa_K145201
location: https://synbiohub.org/public/igem/BBa_K145201/annotation2254368/range2254368/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K145201/annotation2254368/range2254368/1
type: Range
persistent identity: range2254368
display id: range2254368
version: 1
direction: direction/reverse
top level: BBa_K145201
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2254370
display id: annotation2254370
version: 1
top level: BBa_K145201
location: https://synbiohub.org/public/igem/BBa_K145201/annotation2254370/range2254370/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K145201/annotation2254370/range2254370/1
type: Range
persistent identity: range2254370
display id: range2254370
version: 1
direction: direction/reverse
top level: BBa_K145201
start: 44
end: 55
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2254381
display id: annotation2254381
version: 1
top level: BBa_K145201
location: https://synbiohub.org/public/igem/BBa_K145201/annotation2254381/range2254381/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K145201/annotation2254381/range2254381/1
type: Range
persistent identity: range2254381
display id: range2254381
version: 1
direction: direction/reverse
top level: BBa_K145201
start: 755
end: 883
orientation: inline

BBa_I746382
type: ComponentDefinition
persistent identity: BBa I746382
display id: BBa_I746382
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PSP3 pag inducing PP promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the PSP3 pag activator on the PP promoter. Note that the I746351 does contain a RBS.
mutable notes: I746314 was put upstream of I746322
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746314
component: BBa_I746322
sequence annotation: BBa_I746322
sequence annotation: BBa_I746314
sequence: https://synbiohub.org/public/igem/BBa_I746382_sequence/1

BBa_I746314
type: Component
persistent identity: component2291918
display id: component2291918
version: 1
top level: BBa_I746382
access: public
definition: https://synbiohub.org/public/igem/BBa_I746314/1

BBa_I746322
type: Component
persistent identity: component2291931
display id: component2291931
version: 1
top level: BBa_I746382
access: public
definition: https://synbiohub.org/public/igem/BBa_I746322/1

BBa_I746322
type: SequenceAnnotation
persistent identity: annotation2291931
display id: annotation2291931
version: 1
top level: BBa_I746382
location: https://synbiohub.org/public/igem/BBa_I746382/annotation2291931/range2291931/1
component: BBa_I746322

https://synbiohub.org/public/igem/BBa_I746382/annotation2291931/range2291931/1
type: Range
persistent identity: range2291931
display id: range2291931
version: 1
direction: direction/reverse
top level: BBa_I746382
start: 2333
end: 3307
orientation: inline

BBa_I746314
type: SequenceAnnotation
persistent identity: annotation2291918
display id: annotation2291918
version: 1
top level: BBa_I746382
location: https://synbiohub.org/public/igem/BBa_I746382/annotation2291918/range2291918/1
component: BBa_I746314

https://synbiohub.org/public/igem/BBa_I746382/annotation2291918/range2291918/1
type: Range
persistent identity: range2291918
display id: range2291918
version: 1
direction: direction/reverse
top level: BBa_I746382
start: 1
end: 2324
orientation: inline

amyE 5 IS
type: ComponentDefinition
persistent identity: BBa K143001
display id: BBa_K143001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 5??? Integration Sequence for the amyE locus of B. subtilis
creator: Chris Hirst
created: 2008-08-26T11:00:00Z
modified: 2015-05-08T01:10:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _199_
m user id: 0
owner id: 3475
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (Part BBa_K143002) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium B.subtilis. The AmyE locus was the first locus used for integration into ''B.subtilis'' by Shimotsu and Henner#1 and is still commonly used in vectors such as pDR111#2, pDL#3 and their derivatives. Integration at the AmyE locus removes the ability of ''B.subtilis'' to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn#4. The 5' and 3' integration sequences for the AmyE locus were used to integrate the Imperial 2008 iGEM project primary construct into the ''B.sutbilis'' chromosome. #1 pmid=3019840 #2 pmid=14597697 #3 ''Bacillus'' Genetic Stock Center [www.bgsc.org] #4 Cutting, S M.; Vander-Horn, P B. Genetic analysis. In: Harwood C R, Cutting S M. , editors. Molecular biological methods for Bacillus. Chichester, England: John Wiley & Sons, Ltd.; 1990. pp. 27???74.
mutable notes: The AmyE integration sequence was taken from the vector after comparison by BLAST to the ''B.subtilis'' chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 5' end of the gene is the 5' sequence required for integration.
mutable provenance: The 5??? integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on ''B.subtilis'', in particular in the studies of transcriptional control#1 #2 #3 #1 pmid=14597697 #2 pmid=15937167 #3 pmid=12169614
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 5' AmyE homologous sequence
sequence: https://synbiohub.org/public/igem/BBa_K143001_sequence/1

5' AmyE homologous sequence
type: SequenceAnnotation
persistent identity: annotation1974145
display id: annotation1974145
version: 1
top level: amyE 5 IS
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1

https://synbiohub.org/public/igem/BBa_K143001/annotation1974145/range1974145/1
type: Range
persistent identity: range1974145
display id: range1974145
version: 1
direction: direction/forward
top level: amyE 5 IS
start: 1
end: 522
orientation: inline

BBa_I718012
type: ComponentDefinition
persistent identity: BBa I718012
display id: BBa_I718012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ptet-lox71-ter
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Upstream construct of the Synthetics Multicellular Bacterium
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0010
component: BBa_B0012
component: BBa_R0040
component: BBa_I718017
sequence annotation: BBa_I718017
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I718012_sequence/1

BBa_B0010
type: Component
persistent identity: component1956342
display id: component1956342
version: 1
top level: BBa_I718012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1956344
display id: component1956344
version: 1
top level: BBa_I718012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component1956337
display id: component1956337
version: 1
top level: BBa_I718012
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_I718017
type: Component
persistent identity: component1956341
display id: component1956341
version: 1
top level: BBa_I718012
access: public
definition: lox71

BBa_I718017
type: SequenceAnnotation
persistent identity: annotation1956341
display id: annotation1956341
version: 1
top level: BBa_I718012
location: https://synbiohub.org/public/igem/BBa_I718012/annotation1956341/range1956341/1
component: BBa_I718017

https://synbiohub.org/public/igem/BBa_I718012/annotation1956341/range1956341/1
type: Range
persistent identity: range1956341
display id: range1956341
version: 1
direction: direction/reverse
top level: BBa_I718012
start: 63
end: 96
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1956337
display id: annotation1956337
version: 1
top level: BBa_I718012
location: https://synbiohub.org/public/igem/BBa_I718012/annotation1956337/range1956337/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I718012/annotation1956337/range1956337/1
type: Range
persistent identity: range1956337
display id: range1956337
version: 1
direction: direction/forward
top level: BBa_I718012
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956342
display id: annotation1956342
version: 1
top level: BBa_I718012
location: https://synbiohub.org/public/igem/BBa_I718012/annotation1956342/range1956342/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I718012/annotation1956342/range1956342/1
type: Range
persistent identity: range1956342
display id: range1956342
version: 1
direction: direction/forward
top level: BBa_I718012
start: 105
end: 184
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956344
display id: annotation1956344
version: 1
top level: BBa_I718012
location: https://synbiohub.org/public/igem/BBa_I718012/annotation1956344/range1956344/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I718012/annotation1956344/range1956344/1
type: Range
persistent identity: range1956344
display id: range1956344
version: 1
direction: direction/forward
top level: BBa_I718012
start: 193
end: 233
orientation: inline

BBa_I0466
type: ComponentDefinition
persistent identity: BBa I0466
display id: BBa_I0466
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR Protein Generator
creator: mit
created: 2004-07-12T11:00:00Z
modified: 2015-08-31T04:07:29Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR can sense N-butyryl-HSL in the media and can activate transcription from R0071. It can be used as part of a receiver device.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_C0071
sequence annotation: BBa_C0071
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0466_sequence/1

BBa_B0010
type: Component
persistent identity: component943320
display id: component943320
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component943304
display id: component943304
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component943330
display id: component943330
version: 1
top level: BBa_I0466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0071
type: Component
persistent identity: component943314
display id: component943314
version: 1
top level: BBa_I0466
access: public
definition: rhlR

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation943314
display id: annotation943314
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943314/range943314/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_I0466/annotation943314/range943314/1
type: Range
persistent identity: range943314
display id: range943314
version: 1
direction: direction/forward
top level: BBa_I0466
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943320
display id: annotation943320
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943320/range943320/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0466/annotation943320/range943320/1
type: Range
persistent identity: range943320
display id: range943320
version: 1
direction: direction/forward
top level: BBa_I0466
start: 814
end: 893
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943304
display id: annotation943304
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943304/range943304/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0466/annotation943304/range943304/1
type: Range
persistent identity: range943304
display id: range943304
version: 1
direction: direction/forward
top level: BBa_I0466
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943330
display id: annotation943330
version: 1
top level: BBa_I0466
location: https://synbiohub.org/public/igem/BBa_I0466/annotation943330/range943330/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0466/annotation943330/range943330/1
type: Range
persistent identity: range943330
display id: range943330
version: 1
direction: direction/forward
top level: BBa_I0466
start: 902
end: 942
orientation: inline

BBa_I15018
type: ComponentDefinition
persistent identity: BBa I15018
display id: BBa_I15018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: polycistronic ECFP+EYFP under B0031
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to produce a single mRNA encoding ECFP and EYFP under weak RBS B0031
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_E0020
component: BBa_E0030
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_E0030
sequence annotation: BBa_E0020
sequence: https://synbiohub.org/public/igem/BBa_I15018_sequence/1

BBa_E0020
type: Component
persistent identity: component1478442
display id: component1478442
version: 1
top level: BBa_I15018
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_E0030
type: Component
persistent identity: component1478449
display id: component1478449
version: 1
top level: BBa_I15018
access: public
definition: eyfp

BBa_B0031
type: Component
persistent identity: component1478440
display id: component1478440
version: 1
top level: BBa_I15018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0031
type: Component
persistent identity: component1478447
display id: component1478447
version: 1
top level: BBa_I15018
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1478447
display id: annotation1478447
version: 1
top level: BBa_I15018
location: https://synbiohub.org/public/igem/BBa_I15018/annotation1478447/range1478447/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I15018/annotation1478447/range1478447/1
type: Range
persistent identity: range1478447
display id: range1478447
version: 1
direction: direction/forward
top level: BBa_I15018
start: 752
end: 765
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1478449
display id: annotation1478449
version: 1
top level: BBa_I15018
location: https://synbiohub.org/public/igem/BBa_I15018/annotation1478449/range1478449/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I15018/annotation1478449/range1478449/1
type: Range
persistent identity: range1478449
display id: range1478449
version: 1
direction: direction/forward
top level: BBa_I15018
start: 772
end: 1494
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1478442
display id: annotation1478442
version: 1
top level: BBa_I15018
location: https://synbiohub.org/public/igem/BBa_I15018/annotation1478442/range1478442/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I15018/annotation1478442/range1478442/1
type: Range
persistent identity: range1478442
display id: range1478442
version: 1
direction: direction/forward
top level: BBa_I15018
start: 21
end: 743
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation1478440
display id: annotation1478440
version: 1
top level: BBa_I15018
location: https://synbiohub.org/public/igem/BBa_I15018/annotation1478440/range1478440/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I15018/annotation1478440/range1478440/1
type: Range
persistent identity: range1478440
display id: range1478440
version: 1
direction: direction/forward
top level: BBa_I15018
start: 1
end: 14
orientation: inline

BBa_S03813
type: ComponentDefinition
persistent identity: BBa S03813
display id: BBa_S03813
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S0103:J06602
creator: Kenichiro
created: 2007-10-14T11:00:00Z
modified: 2015-05-08T01:14:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 2188
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S0103
component: BBa_J06602
sequence annotation: BBa_J06602
sequence annotation: BBa_S0103
sequence: https://synbiohub.org/public/igem/BBa_S03813_sequence/1

BBa_S0103
type: Component
persistent identity: component2248901
display id: component2248901
version: 1
top level: BBa_S03813
access: public
definition: BBa_S0103

BBa_J06602
type: Component
persistent identity: component2248907
display id: component2248907
version: 1
top level: BBa_S03813
access: public
definition: https://synbiohub.org/public/igem/BBa_J06602/1

BBa_J06602
type: SequenceAnnotation
persistent identity: annotation2248907
display id: annotation2248907
version: 1
top level: BBa_S03813
location: https://synbiohub.org/public/igem/BBa_S03813/annotation2248907/range2248907/1
component: BBa_J06602

https://synbiohub.org/public/igem/BBa_S03813/annotation2248907/range2248907/1
type: Range
persistent identity: range2248907
display id: range2248907
version: 1
direction: direction/reverse
top level: BBa_S03813
start: 802
end: 1533
orientation: inline

BBa_S0103
type: SequenceAnnotation
persistent identity: annotation2248901
display id: annotation2248901
version: 1
top level: BBa_S03813
location: https://synbiohub.org/public/igem/BBa_S03813/annotation2248901/range2248901/1
component: BBa_S0103

https://synbiohub.org/public/igem/BBa_S03813/annotation2248901/range2248901/1
type: Range
persistent identity: range2248901
display id: range2248901
version: 1
direction: direction/reverse
top level: BBa_S03813
start: 1
end: 793
orientation: inline

BBa_I732085
type: ComponentDefinition
persistent identity: BBa I732085
display id: BBa_I732085
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tet repressor generator (version1, TERM+)
creator: Zhan Jian
created: 2007-10-16T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: There is a mutation induced by PCR in [http://partsregistry.org/wiki/index.php/Part:BBa_I732084 BBa_I732084]. But we are convinced that the mutation has nothing to do.
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_I732084
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I732084
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732085_sequence/1

BBa_B0010
type: Component
persistent identity: component1948628
display id: component1948628
version: 1
top level: BBa_I732085
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I732084
type: Component
persistent identity: component1948627
display id: component1948627
version: 1
top level: BBa_I732085
access: public
definition: https://synbiohub.org/public/igem/BBa_I732084/1

BBa_B0012
type: Component
persistent identity: component1948630
display id: component1948630
version: 1
top level: BBa_I732085
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1948628
display id: annotation1948628
version: 1
top level: BBa_I732085
location: https://synbiohub.org/public/igem/BBa_I732085/annotation1948628/range1948628/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732085/annotation1948628/range1948628/1
type: Range
persistent identity: range1948628
display id: range1948628
version: 1
direction: direction/forward
top level: BBa_I732085
start: 698
end: 777
orientation: inline

BBa_I732084
type: SequenceAnnotation
persistent identity: annotation1948627
display id: annotation1948627
version: 1
top level: BBa_I732085
location: https://synbiohub.org/public/igem/BBa_I732085/annotation1948627/range1948627/1
component: BBa_I732084

https://synbiohub.org/public/igem/BBa_I732085/annotation1948627/range1948627/1
type: Range
persistent identity: range1948627
display id: range1948627
version: 1
direction: direction/reverse
top level: BBa_I732085
start: 1
end: 689
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1948630
display id: annotation1948630
version: 1
top level: BBa_I732085
location: https://synbiohub.org/public/igem/BBa_I732085/annotation1948630/range1948630/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732085/annotation1948630/range1948630/1
type: Range
persistent identity: range1948630
display id: range1948630
version: 1
direction: direction/forward
top level: BBa_I732085
start: 786
end: 826
orientation: inline

BBa_J04871
type: ComponentDefinition
persistent identity: BBa J04871
display id: BBa_J04871
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BBa_J04800 (RevAptRibo) Riboswitch with BBa_J04671 (EYFP, RBS-, LVA+, B0015)
creator: Andrew Drysdale and Kristen DeCelle (also Oscar Hernandez and Matt Gemberling)
created: 2005-11-02T12:00:00Z
modified: 2015-08-31T04:08:15Z
discontinued: false
dominant: false
group u list: _16_
m user id: 0
owner id: 435
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is a (RevAptRibo) Riboswitch device that contains a EYFP reporter region with an LVA tag and a B0015 Terminator Sequence
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J04800
component: BBa_B0010
component: BBa_B0012
component: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_J04800
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J04871_sequence/1

BBa_J04800
type: Component
persistent identity: component1735041
display id: component1735041
version: 1
top level: BBa_J04871
access: public
definition: https://synbiohub.org/public/igem/BBa_J04800/1

BBa_B0010
type: Component
persistent identity: component1735061
display id: component1735061
version: 1
top level: BBa_J04871
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1735071
display id: component1735071
version: 1
top level: BBa_J04871
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0032
type: Component
persistent identity: component1735053
display id: component1735053
version: 1
top level: BBa_J04871
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1735061
display id: annotation1735061
version: 1
top level: BBa_J04871
location: https://synbiohub.org/public/igem/BBa_J04871/annotation1735061/range1735061/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04871/annotation1735061/range1735061/1
type: Range
persistent identity: range1735061
display id: range1735061
version: 1
direction: direction/forward
top level: BBa_J04871
start: 1035
end: 1114
orientation: inline

BBa_J04800
type: SequenceAnnotation
persistent identity: annotation1735041
display id: annotation1735041
version: 1
top level: BBa_J04871
location: https://synbiohub.org/public/igem/BBa_J04871/annotation1735041/range1735041/1
component: BBa_J04800

https://synbiohub.org/public/igem/BBa_J04871/annotation1735041/range1735041/1
type: Range
persistent identity: range1735041
display id: range1735041
version: 1
direction: direction/forward
top level: BBa_J04871
start: 1
end: 258
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1735053
display id: annotation1735053
version: 1
top level: BBa_J04871
location: https://synbiohub.org/public/igem/BBa_J04871/annotation1735053/range1735053/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_J04871/annotation1735053/range1735053/1
type: Range
persistent identity: range1735053
display id: range1735053
version: 1
direction: direction/forward
top level: BBa_J04871
start: 265
end: 1026
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1735071
display id: annotation1735071
version: 1
top level: BBa_J04871
location: https://synbiohub.org/public/igem/BBa_J04871/annotation1735071/range1735071/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04871/annotation1735071/range1735071/1
type: Range
persistent identity: range1735071
display id: range1735071
version: 1
direction: direction/forward
top level: BBa_J04871
start: 1123
end: 1163
orientation: inline

BBa_S01013
type: ComponentDefinition
persistent identity: BBa S01013
display id: BBa_S01013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0079.E0420
creator: Randy Rettberg
created: 2005-06-10T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from Jen 1 tube 82
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_C0079
component: BBa_B0034
component: BBa_E0020
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0079
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence: https://synbiohub.org/public/igem/BBa_S01013_sequence/1

BBa_B0012
type: Component
persistent identity: component1511627
display id: component1511627
version: 1
top level: BBa_S01013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0079
type: Component
persistent identity: component1511604
display id: component1511604
version: 1
top level: BBa_S01013
access: public
definition: lasr

BBa_B0034
type: Component
persistent identity: component1511610
display id: component1511610
version: 1
top level: BBa_S01013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component1511591
display id: component1511591
version: 1
top level: BBa_S01013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: Component
persistent identity: component1511612
display id: component1511612
version: 1
top level: BBa_S01013
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component1511617
display id: component1511617
version: 1
top level: BBa_S01013
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1511627
display id: annotation1511627
version: 1
top level: BBa_S01013
location: https://synbiohub.org/public/igem/BBa_S01013/annotation1511627/range1511627/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01013/annotation1511627/range1511627/1
type: Range
persistent identity: range1511627
display id: range1511627
version: 1
direction: direction/forward
top level: BBa_S01013
start: 1645
end: 1685
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation1511604
display id: annotation1511604
version: 1
top level: BBa_S01013
location: https://synbiohub.org/public/igem/BBa_S01013/annotation1511604/range1511604/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_S01013/annotation1511604/range1511604/1
type: Range
persistent identity: range1511604
display id: range1511604
version: 1
direction: direction/forward
top level: BBa_S01013
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1511617
display id: annotation1511617
version: 1
top level: BBa_S01013
location: https://synbiohub.org/public/igem/BBa_S01013/annotation1511617/range1511617/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01013/annotation1511617/range1511617/1
type: Range
persistent identity: range1511617
display id: range1511617
version: 1
direction: direction/forward
top level: BBa_S01013
start: 1557
end: 1636
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511591
display id: annotation1511591
version: 1
top level: BBa_S01013
location: https://synbiohub.org/public/igem/BBa_S01013/annotation1511591/range1511591/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01013/annotation1511591/range1511591/1
type: Range
persistent identity: range1511591
display id: range1511591
version: 1
direction: direction/forward
top level: BBa_S01013
start: 1
end: 12
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1511612
display id: annotation1511612
version: 1
top level: BBa_S01013
location: https://synbiohub.org/public/igem/BBa_S01013/annotation1511612/range1511612/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_S01013/annotation1511612/range1511612/1
type: Range
persistent identity: range1511612
display id: range1511612
version: 1
direction: direction/forward
top level: BBa_S01013
start: 826
end: 1548
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511610
display id: annotation1511610
version: 1
top level: BBa_S01013
location: https://synbiohub.org/public/igem/BBa_S01013/annotation1511610/range1511610/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01013/annotation1511610/range1511610/1
type: Range
persistent identity: range1511610
display id: range1511610
version: 1
direction: direction/forward
top level: BBa_S01013
start: 808
end: 819
orientation: inline

BBa_I746360
type: ComponentDefinition
persistent identity: BBa I746360
display id: BBa_I746360
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PF promoter from P2 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.
mutable notes: no special considerations seemed necessary
mutable provenance: The original source is the P2 phage genome. For our work the DNA came from plasmids, kindly supplied to us by Prof. Calendar, University of California (Berkeley): Bacteriophage PSP3 and fR73 Activator Proteins: Analysis of Promoter Specificities; Julien and Calendar, 1996; JOURNAL OF BACTERIOLOGY, Oct. 1996, p. 5668???5675
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PF
sequence: https://synbiohub.org/public/igem/BBa_I746360_sequence/1

PF
type: SequenceAnnotation
persistent identity: annotation1943783
display id: annotation1943783
version: 1
top level: BBa_I746360
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1

https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1
type: Range
persistent identity: range1943783
display id: range1943783
version: 1
direction: direction/forward
top level: BBa_I746360
start: 1
end: 91
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0024
display id: BBa_E0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0024_sequence/1

BBa_J45397
type: ComponentDefinition
persistent identity: BBa J45397
display id: BBa_J45397
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + BAT2 + RBS
creator: Andr?? Green II
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is an intermediate to the protein generator BBa_45400.
mutable notes: coming
mutable provenance: coming
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J45008
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_J45008
sequence: https://synbiohub.org/public/igem/BBa_J45397_sequence/1

BBa_J45008
type: Component
persistent identity: component1907924
display id: component1907924
version: 1
top level: BBa_J45397
access: public
definition: https://synbiohub.org/public/igem/BBa_J45008/1

BBa_B0030
type: Component
persistent identity: component1907919
display id: component1907919
version: 1
top level: BBa_J45397
access: public
definition: BBa_B0030

BBa_B0030
type: Component
persistent identity: component1907926
display id: component1907926
version: 1
top level: BBa_J45397
access: public
definition: BBa_B0030

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1907919
display id: annotation1907919
version: 1
top level: BBa_J45397
location: https://synbiohub.org/public/igem/BBa_J45397/annotation1907919/range1907919/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45397/annotation1907919/range1907919/1
type: Range
persistent identity: range1907919
display id: range1907919
version: 1
direction: direction/forward
top level: BBa_J45397
start: 1
end: 15
orientation: inline

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1907924
display id: annotation1907924
version: 1
top level: BBa_J45397
location: https://synbiohub.org/public/igem/BBa_J45397/annotation1907924/range1907924/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45397/annotation1907924/range1907924/1
type: Range
persistent identity: range1907924
display id: range1907924
version: 1
direction: direction/reverse
top level: BBa_J45397
start: 22
end: 1155
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1907926
display id: annotation1907926
version: 1
top level: BBa_J45397
location: https://synbiohub.org/public/igem/BBa_J45397/annotation1907926/range1907926/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45397/annotation1907926/range1907926/1
type: Range
persistent identity: range1907926
display id: range1907926
version: 1
direction: direction/forward
top level: BBa_J45397
start: 1164
end: 1178
orientation: inline

BBa_S03154
type: ComponentDefinition
persistent identity: BBa S03154
display id: BBa_S03154
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0078
creator: Jennifer Braff
created: 2004-07-22T11:00:00Z
modified: 2015-05-08T01:14:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0078
component: BBa_B0034
sequence annotation: BBa_C0078
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03154_sequence/1

BBa_C0078
type: Component
persistent identity: component940972
display id: component940972
version: 1
top level: BBa_S03154
access: public
definition: lasI

BBa_B0034
type: Component
persistent identity: component940962
display id: component940962
version: 1
top level: BBa_S03154
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation940972
display id: annotation940972
version: 1
top level: BBa_S03154
location: https://synbiohub.org/public/igem/BBa_S03154/annotation940972/range940972/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_S03154/annotation940972/range940972/1
type: Range
persistent identity: range940972
display id: range940972
version: 1
direction: direction/forward
top level: BBa_S03154
start: 19
end: 660
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation940962
display id: annotation940962
version: 1
top level: BBa_S03154
location: https://synbiohub.org/public/igem/BBa_S03154/annotation940962/range940962/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03154/annotation940962/range940962/1
type: Range
persistent identity: range940962
display id: range940962
version: 1
direction: direction/forward
top level: BBa_S03154
start: 1
end: 12
orientation: inline

BBa_I11031
type: ComponentDefinition
persistent identity: BBa I11031
display id: BBa_I11031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: excisionase from E. coli phage P22 (removes prophage from host genome)
creator: mschomp
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: P22 Excisionase with AAV tag
mutable provenance: Nuc Acid Res, 2001; 29(11):2205-2216
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: stop
sequence annotation: Xis p22
sequence annotation: AAV
sequence: https://synbiohub.org/public/igem/BBa_I11031_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214030
display id: annotation2214030
version: 1
top level: BBa_I11031
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I11031/annotation2214030/range2214030/1

https://synbiohub.org/public/igem/BBa_I11031/annotation2214030/range2214030/1
type: Range
persistent identity: range2214030
display id: range2214030
version: 1
direction: direction/forward
top level: BBa_I11031
start: 388
end: 412
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1198221
display id: annotation1198221
version: 1
top level: BBa_I11031
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11031/annotation1198221/range1198221/1

https://synbiohub.org/public/igem/BBa_I11031/annotation1198221/range1198221/1
type: Range
persistent identity: range1198221
display id: range1198221
version: 1
direction: direction/forward
top level: BBa_I11031
start: 382
end: 387
orientation: inline

Xis p22
type: SequenceAnnotation
persistent identity: annotation1198186
display id: annotation1198186
version: 1
top level: BBa_I11031
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11031/annotation1198186/range1198186/1

https://synbiohub.org/public/igem/BBa_I11031/annotation1198186/range1198186/1
type: Range
persistent identity: range1198186
display id: range1198186
version: 1
direction: direction/forward
top level: BBa_I11031
start: 1
end: 348
orientation: inline

AAV
type: SequenceAnnotation
persistent identity: annotation1064115
display id: annotation1064115
version: 1
top level: BBa_I11031
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I11031/annotation1064115/range1064115/1

https://synbiohub.org/public/igem/BBa_I11031/annotation1064115/range1064115/1
type: Range
persistent identity: range1064115
display id: range1064115
version: 1
direction: direction/forward
top level: BBa_I11031
start: 349
end: 381
orientation: inline

Cat
type: ComponentDefinition
persistent identity: BBa I14033
display id: BBa_I14033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Cat)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Constitutive Promoter, Medium Transcription
mutable provenance: Plasmid pACYC184
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(Cat)
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_I14033_sequence/1

P(Cat)
type: SequenceAnnotation
persistent identity: annotation1026174
display id: annotation1026174
version: 1
top level: Cat
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1

https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1
type: Range
persistent identity: range1026174
display id: range1026174
version: 1
direction: direction/forward
top level: Cat
start: 1
end: 38
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026173
display id: annotation1026173
version: 1
top level: Cat
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1

https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1
type: Range
persistent identity: range1026173
display id: range1026173
version: 1
direction: direction/forward
top level: Cat
start: 27
end: 32
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026172
display id: annotation1026172
version: 1
top level: Cat
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1

https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1
type: Range
persistent identity: range1026172
display id: range1026172
version: 1
direction: direction/forward
top level: Cat
start: 4
end: 9
orientation: inline

BBa_I732835
type: ComponentDefinition
persistent identity: BBa I732835
display id: BBa_I732835
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL4A0604 (RBS+, TERM+)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:08:00Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_I732115
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_I732115
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I732835_sequence/1

BBa_B0012
type: Component
persistent identity: component1946881
display id: component1946881
version: 1
top level: BBa_I732835
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I732115
type: Component
persistent identity: component1946878
display id: component1946878
version: 1
top level: BBa_I732835
access: public
definition: ARL4A0604

BBa_B0010
type: Component
persistent identity: component1946879
display id: component1946879
version: 1
top level: BBa_I732835
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1946877
display id: component1946877
version: 1
top level: BBa_I732835
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1946879
display id: annotation1946879
version: 1
top level: BBa_I732835
location: https://synbiohub.org/public/igem/BBa_I732835/annotation1946879/range1946879/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732835/annotation1946879/range1946879/1
type: Range
persistent identity: range1946879
display id: range1946879
version: 1
direction: direction/forward
top level: BBa_I732835
start: 1113
end: 1192
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946877
display id: annotation1946877
version: 1
top level: BBa_I732835
location: https://synbiohub.org/public/igem/BBa_I732835/annotation1946877/range1946877/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732835/annotation1946877/range1946877/1
type: Range
persistent identity: range1946877
display id: range1946877
version: 1
direction: direction/reverse
top level: BBa_I732835
start: 1
end: 12
orientation: inline

BBa_I732115
type: SequenceAnnotation
persistent identity: annotation1946878
display id: annotation1946878
version: 1
top level: BBa_I732835
location: https://synbiohub.org/public/igem/BBa_I732835/annotation1946878/range1946878/1
component: BBa_I732115

https://synbiohub.org/public/igem/BBa_I732835/annotation1946878/range1946878/1
type: Range
persistent identity: range1946878
display id: range1946878
version: 1
direction: direction/reverse
top level: BBa_I732835
start: 19
end: 1104
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1946881
display id: annotation1946881
version: 1
top level: BBa_I732835
location: https://synbiohub.org/public/igem/BBa_I732835/annotation1946881/range1946881/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732835/annotation1946881/range1946881/1
type: Range
persistent identity: range1946881
display id: range1946881
version: 1
direction: direction/forward
top level: BBa_I732835
start: 1201
end: 1241
orientation: inline

BBa_S03156
type: ComponentDefinition
persistent identity: BBa S03156
display id: BBa_S03156
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0079
creator: Jennifer Braff
created: 2004-07-22T11:00:00Z
modified: 2015-05-08T01:14:20Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0079
component: BBa_B0034
sequence annotation: BBa_C0079
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S03156_sequence/1

BBa_C0079
type: Component
persistent identity: component940989
display id: component940989
version: 1
top level: BBa_S03156
access: public
definition: lasr

BBa_B0034
type: Component
persistent identity: component940976
display id: component940976
version: 1
top level: BBa_S03156
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation940989
display id: annotation940989
version: 1
top level: BBa_S03156
location: https://synbiohub.org/public/igem/BBa_S03156/annotation940989/range940989/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_S03156/annotation940989/range940989/1
type: Range
persistent identity: range940989
display id: range940989
version: 1
direction: direction/forward
top level: BBa_S03156
start: 19
end: 774
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation940976
display id: annotation940976
version: 1
top level: BBa_S03156
location: https://synbiohub.org/public/igem/BBa_S03156/annotation940976/range940976/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03156/annotation940976/range940976/1
type: Range
persistent identity: range940976
display id: range940976
version: 1
direction: direction/forward
top level: BBa_S03156
start: 1
end: 12
orientation: inline

LRLa
type: ComponentDefinition
persistent identity: BBa I732101
display id: BBa_I732101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LRLa
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732101_sequence/1

BBa_S01012
type: ComponentDefinition
persistent identity: BBa S01012
display id: BBa_S01012
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0078.E0420
creator: Randy Rettberg
created: 2005-06-10T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from Jen 1 tube 83
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0034
component: BBa_E0020
component: BBa_B0010
component: BBa_C0078
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_C0078
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S01012_sequence/1

BBa_B0012
type: Component
persistent identity: component1511585
display id: component1511585
version: 1
top level: BBa_S01012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1511552
display id: component1511552
version: 1
top level: BBa_S01012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: Component
persistent identity: component1511570
display id: component1511570
version: 1
top level: BBa_S01012
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component1511575
display id: component1511575
version: 1
top level: BBa_S01012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1511568
display id: component1511568
version: 1
top level: BBa_S01012
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0078
type: Component
persistent identity: component1511562
display id: component1511562
version: 1
top level: BBa_S01012
access: public
definition: lasI

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1511570
display id: annotation1511570
version: 1
top level: BBa_S01012
location: https://synbiohub.org/public/igem/BBa_S01012/annotation1511570/range1511570/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_S01012/annotation1511570/range1511570/1
type: Range
persistent identity: range1511570
display id: range1511570
version: 1
direction: direction/forward
top level: BBa_S01012
start: 712
end: 1434
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511552
display id: annotation1511552
version: 1
top level: BBa_S01012
location: https://synbiohub.org/public/igem/BBa_S01012/annotation1511552/range1511552/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01012/annotation1511552/range1511552/1
type: Range
persistent identity: range1511552
display id: range1511552
version: 1
direction: direction/forward
top level: BBa_S01012
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1511575
display id: annotation1511575
version: 1
top level: BBa_S01012
location: https://synbiohub.org/public/igem/BBa_S01012/annotation1511575/range1511575/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01012/annotation1511575/range1511575/1
type: Range
persistent identity: range1511575
display id: range1511575
version: 1
direction: direction/forward
top level: BBa_S01012
start: 1443
end: 1522
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation1511562
display id: annotation1511562
version: 1
top level: BBa_S01012
location: https://synbiohub.org/public/igem/BBa_S01012/annotation1511562/range1511562/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_S01012/annotation1511562/range1511562/1
type: Range
persistent identity: range1511562
display id: range1511562
version: 1
direction: direction/forward
top level: BBa_S01012
start: 19
end: 660
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1511585
display id: annotation1511585
version: 1
top level: BBa_S01012
location: https://synbiohub.org/public/igem/BBa_S01012/annotation1511585/range1511585/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01012/annotation1511585/range1511585/1
type: Range
persistent identity: range1511585
display id: range1511585
version: 1
direction: direction/forward
top level: BBa_S01012
start: 1531
end: 1571
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511568
display id: annotation1511568
version: 1
top level: BBa_S01012
location: https://synbiohub.org/public/igem/BBa_S01012/annotation1511568/range1511568/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01012/annotation1511568/range1511568/1
type: Range
persistent identity: range1511568
display id: range1511568
version: 1
direction: direction/forward
top level: BBa_S01012
start: 694
end: 705
orientation: inline

BBa_I13028
type: ComponentDefinition
persistent identity: BBa I13028
display id: BBa_I13028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI (TP-901) QPI intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0034
component: BBa_C0075
sequence annotation: BBa_C0075
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I13028_sequence/1

BBa_B0034
type: Component
persistent identity: component939462
display id: component939462
version: 1
top level: BBa_I13028
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0075
type: Component
persistent identity: component939472
display id: component939472
version: 1
top level: BBa_I13028
access: public
definition: cI TP901

BBa_C0075
type: SequenceAnnotation
persistent identity: annotation939472
display id: annotation939472
version: 1
top level: BBa_I13028
location: https://synbiohub.org/public/igem/BBa_I13028/annotation939472/range939472/1
component: BBa_C0075

https://synbiohub.org/public/igem/BBa_I13028/annotation939472/range939472/1
type: Range
persistent identity: range939472
display id: range939472
version: 1
direction: direction/forward
top level: BBa_I13028
start: 19
end: 597
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939462
display id: annotation939462
version: 1
top level: BBa_I13028
location: https://synbiohub.org/public/igem/BBa_I13028/annotation939462/range939462/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13028/annotation939462/range939462/1
type: Range
persistent identity: range939462
display id: range939462
version: 1
direction: direction/forward
top level: BBa_I13028
start: 1
end: 12
orientation: inline

BBa_I714031
type: ComponentDefinition
persistent identity: BBa I714031
display id: BBa_I714031
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriT-R (Origin of Transfer for the R751-plasmid nic region)
creator: Mingzhi Qu
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: OriT-R751 (Origin of Transfer for the R751-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by R751 plasmid machinery begins via rolling circle replication.
mutable provenance: Enterobacter aerogenes plasmid R751 NCBI:NC_001735
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_I714031_sequence/1

BBa_S03827
type: ComponentDefinition
persistent identity: BBa S03827
display id: BBa_S03827
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I722005:A340620
creator: Zhu Manli, Cao Yingxiu, Wang Xing
created: 2007-10-19T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 1532
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_A340620
component: BBa_I722005
sequence annotation: BBa_A340620
sequence annotation: BBa_I722005
sequence: https://synbiohub.org/public/igem/BBa_S03827_sequence/1

BBa_A340620
type: Component
persistent identity: component2299159
display id: component2299159
version: 1
top level: BBa_S03827
access: public
definition: BBa_A340620

BBa_I722005
type: Component
persistent identity: component2299139
display id: component2299139
version: 1
top level: BBa_S03827
access: public
definition: https://synbiohub.org/public/igem/BBa_I722005/1

BBa_A340620
type: SequenceAnnotation
persistent identity: annotation2299159
display id: annotation2299159
version: 1
top level: BBa_S03827
location: https://synbiohub.org/public/igem/BBa_S03827/annotation2299159/range2299159/1
component: BBa_A340620

https://synbiohub.org/public/igem/BBa_S03827/annotation2299159/range2299159/1
type: Range
persistent identity: range2299159
display id: range2299159
version: 1
direction: direction/reverse
top level: BBa_S03827
start: 1873
end: 2871
orientation: inline

BBa_I722005
type: SequenceAnnotation
persistent identity: annotation2299139
display id: annotation2299139
version: 1
top level: BBa_S03827
location: https://synbiohub.org/public/igem/BBa_S03827/annotation2299139/range2299139/1
component: BBa_I722005

https://synbiohub.org/public/igem/BBa_S03827/annotation2299139/range2299139/1
type: Range
persistent identity: range2299139
display id: range2299139
version: 1
direction: direction/reverse
top level: BBa_S03827
start: 1
end: 1864
orientation: inline

BBa_S03184
type: ComponentDefinition
persistent identity: BBa S03184
display id: BBa_S03184
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015.I6108
creator: cconboy
created: 2004-07-06T11:00:00Z
modified: 2015-05-08T01:14:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0080
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0080
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_S03184_sequence/1

BBa_R0080
type: Component
persistent identity: component959416
display id: component959416
version: 1
top level: BBa_S03184
access: public
definition: AraC

BBa_B0012
type: Component
persistent identity: component959393
display id: component959393
version: 1
top level: BBa_S03184
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component959383
display id: component959383
version: 1
top level: BBa_S03184
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component959421
display id: component959421
version: 1
top level: BBa_S03184
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component959438
display id: component959438
version: 1
top level: BBa_S03184
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0030
type: Component
persistent identity: component959423
display id: component959423
version: 1
top level: BBa_S03184
access: public
definition: eyfp

BBa_B0010
type: Component
persistent identity: component959428
display id: component959428
version: 1
top level: BBa_S03184
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation959428
display id: annotation959428
version: 1
top level: BBa_S03184
location: https://synbiohub.org/public/igem/BBa_S03184/annotation959428/range959428/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03184/annotation959428/range959428/1
type: Range
persistent identity: range959428
display id: range959428
version: 1
direction: direction/forward
top level: BBa_S03184
start: 1044
end: 1123
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation959438
display id: annotation959438
version: 1
top level: BBa_S03184
location: https://synbiohub.org/public/igem/BBa_S03184/annotation959438/range959438/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03184/annotation959438/range959438/1
type: Range
persistent identity: range959438
display id: range959438
version: 1
direction: direction/forward
top level: BBa_S03184
start: 1132
end: 1172
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation959393
display id: annotation959393
version: 1
top level: BBa_S03184
location: https://synbiohub.org/public/igem/BBa_S03184/annotation959393/range959393/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03184/annotation959393/range959393/1
type: Range
persistent identity: range959393
display id: range959393
version: 1
direction: direction/forward
top level: BBa_S03184
start: 89
end: 129
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation959416
display id: annotation959416
version: 1
top level: BBa_S03184
location: https://synbiohub.org/public/igem/BBa_S03184/annotation959416/range959416/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_S03184/annotation959416/range959416/1
type: Range
persistent identity: range959416
display id: range959416
version: 1
direction: direction/forward
top level: BBa_S03184
start: 138
end: 286
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation959421
display id: annotation959421
version: 1
top level: BBa_S03184
location: https://synbiohub.org/public/igem/BBa_S03184/annotation959421/range959421/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03184/annotation959421/range959421/1
type: Range
persistent identity: range959421
display id: range959421
version: 1
direction: direction/forward
top level: BBa_S03184
start: 295
end: 306
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation959423
display id: annotation959423
version: 1
top level: BBa_S03184
location: https://synbiohub.org/public/igem/BBa_S03184/annotation959423/range959423/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_S03184/annotation959423/range959423/1
type: Range
persistent identity: range959423
display id: range959423
version: 1
direction: direction/forward
top level: BBa_S03184
start: 313
end: 1035
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation959383
display id: annotation959383
version: 1
top level: BBa_S03184
location: https://synbiohub.org/public/igem/BBa_S03184/annotation959383/range959383/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03184/annotation959383/range959383/1
type: Range
persistent identity: range959383
display id: range959383
version: 1
direction: direction/forward
top level: BBa_S03184
start: 1
end: 80
orientation: inline

BBa_E5503
type: ComponentDefinition
persistent identity: BBa E5503
display id: BBa_E5503
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0030.E0040
creator: jcbraff
created: 2005-05-16T11:00:00Z
modified: 2015-08-31T04:07:27Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 61
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0040.B0030.E0040 (R0040.E5500)
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_R0040
component: BBa_E0040
component: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0030
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_E5503_sequence/1

BBa_R0040
type: Component
persistent identity: component1489136
display id: component1489136
version: 1
top level: BBa_E5503
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0040
type: Component
persistent identity: component1489150
display id: component1489150
version: 1
top level: BBa_E5503
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0030
type: Component
persistent identity: component1489147
display id: component1489147
version: 1
top level: BBa_E5503
access: public
definition: BBa_B0030

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1489150
display id: annotation1489150
version: 1
top level: BBa_E5503
location: https://synbiohub.org/public/igem/BBa_E5503/annotation1489150/range1489150/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E5503/annotation1489150/range1489150/1
type: Range
persistent identity: range1489150
display id: range1489150
version: 1
direction: direction/forward
top level: BBa_E5503
start: 84
end: 803
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1489147
display id: annotation1489147
version: 1
top level: BBa_E5503
location: https://synbiohub.org/public/igem/BBa_E5503/annotation1489147/range1489147/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E5503/annotation1489147/range1489147/1
type: Range
persistent identity: range1489147
display id: range1489147
version: 1
direction: direction/forward
top level: BBa_E5503
start: 63
end: 77
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1489136
display id: annotation1489136
version: 1
top level: BBa_E5503
location: https://synbiohub.org/public/igem/BBa_E5503/annotation1489136/range1489136/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_E5503/annotation1489136/range1489136/1
type: Range
persistent identity: range1489136
display id: range1489136
version: 1
direction: direction/forward
top level: BBa_E5503
start: 1
end: 54
orientation: inline

LacY
type: ComponentDefinition
persistent identity: BBa K079015
display id: BBa_K079015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacY transporter protein from E. coli
creator: Francesca Ceroni
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:33Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _185_
m user id: 0
owner id: 3530
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part has been derived from the BBa_J22101 part. The BBa_J22101 part was not working due to an insertion of 35 bp upstream the LacY coding sequence. We amplified the right gene sequence by PCR and put it into a standard plasmid.
mutable notes: Derived from the BBa_J22101 part
mutable provenance: Derived from the BBa_J22101 part
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K079015_sequence/1

cI 434
type: ComponentDefinition
persistent identity: BBa R0052
display id: BBa_R0052
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (434 cI regulated)
creator: Maia Mahoney
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The 434 cI regulatory region sequence is a 89 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. 434 cI repressor protein, <bb_part>BBa_C0052<bb_part>, binds to it. This segment contains O-R1, O-R2, P-R, and P-RM -35.
mutable provenance: Bacteriophage 434 right operator
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: start
sequence annotation: BBa_R0052
sequence: https://synbiohub.org/public/igem/BBa_R0052_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation2028
display id: annotation2028
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2028/range2028/1
type: Range
persistent identity: range2028
display id: range2028
version: 1
direction: direction/forward
top level: cI 434
start: 30
end: 43
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2031
display id: annotation2031
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2031/range2031/1
type: Range
persistent identity: range2031
display id: range2031
version: 1
direction: direction/forward
top level: cI 434
start: 2
end: 7
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2027
display id: annotation2027
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2027/range2027/1
type: Range
persistent identity: range2027
display id: range2027
version: 1
direction: direction/forward
top level: cI 434
start: 8
end: 21
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2029
display id: annotation2029
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2029/range2029/1
type: Range
persistent identity: range2029
display id: range2029
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 6
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2030
display id: annotation2030
version: 1
top level: cI 434
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2030/range2030/1
type: Range
persistent identity: range2030
display id: range2030
version: 1
direction: direction/forward
top level: cI 434
start: 24
end: 29
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2026
display id: annotation2026
version: 1
top level: cI 434
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2026/range2026/1
type: Range
persistent identity: range2026
display id: range2026
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 43
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2032
display id: annotation2032
version: 1
top level: cI 434
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1

https://synbiohub.org/public/igem/BBa_R0052/annotation2032/range2032/1
type: Range
persistent identity: range2032
display id: range2032
version: 1
direction: direction/forward
top level: cI 434
start: 35
end: 37
orientation: inline

BBa_R0052
type: SequenceAnnotation
persistent identity: annotation7068
display id: annotation7068
version: 1
top level: cI 434
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1

https://synbiohub.org/public/igem/BBa_R0052/annotation7068/range7068/1
type: Range
persistent identity: range7068
display id: range7068
version: 1
direction: direction/forward
top level: cI 434
start: 1
end: 46
orientation: inline

BBa_S01004
type: ComponentDefinition
persistent identity: BBa S01004
display id: BBa_S01004
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E0432.R0062.E0422
creator: Randy Rettberg
created: 2005-06-10T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from Jen 1 tube 54
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0022
component: BBa_B0010
component: BBa_B0012
component: BBa_B0034
component: BBa_E0032
component: BBa_R0062
sequence annotation: BBa_E0022
sequence annotation: BBa_E0032
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S01004_sequence/1

BBa_E0022
type: Component
persistent identity: component1511134
display id: component1511134
version: 1
top level: BBa_S01004
access: public
definition: https://synbiohub.org/public/igem/BBa_E0022/1

BBa_B0010
type: Component
persistent identity: component1511142
display id: component1511142
version: 1
top level: BBa_S01004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1511099
display id: component1511099
version: 1
top level: BBa_S01004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1511122
display id: component1511122
version: 1
top level: BBa_S01004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0032
type: Component
persistent identity: component1511081
display id: component1511081
version: 1
top level: BBa_S01004
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0034
type: Component
persistent identity: component1511069
display id: component1511069
version: 1
top level: BBa_S01004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1511089
display id: component1511089
version: 1
top level: BBa_S01004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0062
type: Component
persistent identity: component1511114
display id: component1511114
version: 1
top level: BBa_S01004
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0012
type: Component
persistent identity: component1511152
display id: component1511152
version: 1
top level: BBa_S01004
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1511134
display id: annotation1511134
version: 1
top level: BBa_S01004
location: https://synbiohub.org/public/igem/BBa_S01004/annotation1511134/range1511134/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_S01004/annotation1511134/range1511134/1
type: Range
persistent identity: range1511134
display id: range1511134
version: 1
direction: direction/forward
top level: BBa_S01004
start: 1007
end: 1768
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1511081
display id: annotation1511081
version: 1
top level: BBa_S01004
location: https://synbiohub.org/public/igem/BBa_S01004/annotation1511081/range1511081/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_S01004/annotation1511081/range1511081/1
type: Range
persistent identity: range1511081
display id: range1511081
version: 1
direction: direction/forward
top level: BBa_S01004
start: 19
end: 780
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1511099
display id: annotation1511099
version: 1
top level: BBa_S01004
location: https://synbiohub.org/public/igem/BBa_S01004/annotation1511099/range1511099/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01004/annotation1511099/range1511099/1
type: Range
persistent identity: range1511099
display id: range1511099
version: 1
direction: direction/forward
top level: BBa_S01004
start: 877
end: 917
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1511114
display id: annotation1511114
version: 1
top level: BBa_S01004
location: https://synbiohub.org/public/igem/BBa_S01004/annotation1511114/range1511114/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_S01004/annotation1511114/range1511114/1
type: Range
persistent identity: range1511114
display id: range1511114
version: 1
direction: direction/forward
top level: BBa_S01004
start: 926
end: 980
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511069
display id: annotation1511069
version: 1
top level: BBa_S01004
location: https://synbiohub.org/public/igem/BBa_S01004/annotation1511069/range1511069/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01004/annotation1511069/range1511069/1
type: Range
persistent identity: range1511069
display id: range1511069
version: 1
direction: direction/forward
top level: BBa_S01004
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1511089
display id: annotation1511089
version: 1
top level: BBa_S01004
location: https://synbiohub.org/public/igem/BBa_S01004/annotation1511089/range1511089/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01004/annotation1511089/range1511089/1
type: Range
persistent identity: range1511089
display id: range1511089
version: 1
direction: direction/forward
top level: BBa_S01004
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1511152
display id: annotation1511152
version: 1
top level: BBa_S01004
location: https://synbiohub.org/public/igem/BBa_S01004/annotation1511152/range1511152/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01004/annotation1511152/range1511152/1
type: Range
persistent identity: range1511152
display id: range1511152
version: 1
direction: direction/forward
top level: BBa_S01004
start: 1865
end: 1905
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1511142
display id: annotation1511142
version: 1
top level: BBa_S01004
location: https://synbiohub.org/public/igem/BBa_S01004/annotation1511142/range1511142/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01004/annotation1511142/range1511142/1
type: Range
persistent identity: range1511142
display id: range1511142
version: 1
direction: direction/forward
top level: BBa_S01004
start: 1777
end: 1856
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511122
display id: annotation1511122
version: 1
top level: BBa_S01004
location: https://synbiohub.org/public/igem/BBa_S01004/annotation1511122/range1511122/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01004/annotation1511122/range1511122/1
type: Range
persistent identity: range1511122
display id: range1511122
version: 1
direction: direction/forward
top level: BBa_S01004
start: 989
end: 1000
orientation: inline

BBa_J37032
type: ComponentDefinition
persistent identity: BBa J37032
display id: BBa_J37032
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP expression controlled by LuxR promoter
creator: John Sy
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: true
group u list: _66_
m user id: 0
owner id: 1052
owning group id: 66
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This forms a GFP coding region which is controlled by the pLuxR promoter. This part will be used to form J37020, a test construct for the polycistronic predator cell (Imperial College iGEM 2006)
mutable notes: None
mutable provenance: Parts ligated from existing parts in registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_E0040
component: BBa_B0012
component: BBa_R0062
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J37032_sequence/1

BBa_E0040
type: Component
persistent identity: component1897088
display id: component1897088
version: 1
top level: BBa_J37032
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1897091
display id: component1897091
version: 1
top level: BBa_J37032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0062
type: Component
persistent identity: component1897082
display id: component1897082
version: 1
top level: BBa_J37032
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0010
type: Component
persistent identity: component1897089
display id: component1897089
version: 1
top level: BBa_J37032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1897087
display id: component1897087
version: 1
top level: BBa_J37032
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1897089
display id: annotation1897089
version: 1
top level: BBa_J37032
location: https://synbiohub.org/public/igem/BBa_J37032/annotation1897089/range1897089/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J37032/annotation1897089/range1897089/1
type: Range
persistent identity: range1897089
display id: range1897089
version: 1
direction: direction/forward
top level: BBa_J37032
start: 810
end: 889
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1897082
display id: annotation1897082
version: 1
top level: BBa_J37032
location: https://synbiohub.org/public/igem/BBa_J37032/annotation1897082/range1897082/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_J37032/annotation1897082/range1897082/1
type: Range
persistent identity: range1897082
display id: range1897082
version: 1
direction: direction/forward
top level: BBa_J37032
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1897087
display id: annotation1897087
version: 1
top level: BBa_J37032
location: https://synbiohub.org/public/igem/BBa_J37032/annotation1897087/range1897087/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J37032/annotation1897087/range1897087/1
type: Range
persistent identity: range1897087
display id: range1897087
version: 1
direction: direction/reverse
top level: BBa_J37032
start: 64
end: 75
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1897088
display id: annotation1897088
version: 1
top level: BBa_J37032
location: https://synbiohub.org/public/igem/BBa_J37032/annotation1897088/range1897088/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_J37032/annotation1897088/range1897088/1
type: Range
persistent identity: range1897088
display id: range1897088
version: 1
direction: direction/reverse
top level: BBa_J37032
start: 82
end: 801
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1897091
display id: annotation1897091
version: 1
top level: BBa_J37032
location: https://synbiohub.org/public/igem/BBa_J37032/annotation1897091/range1897091/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J37032/annotation1897091/range1897091/1
type: Range
persistent identity: range1897091
display id: range1897091
version: 1
direction: direction/forward
top level: BBa_J37032
start: 898
end: 938
orientation: inline

BBa_B1007
type: ComponentDefinition
persistent identity: BBa B1007
display id: BBa_B1007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~=80)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~=80% # 8bp stem, 6nt loop # Bidirectional, estimated reverse %T~>90%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 6 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1007
sequence annotation: PolyA
sequence annotation: modified thr
sequence: https://synbiohub.org/public/igem/BBa_B1007_sequence/1

B1007
type: SequenceAnnotation
persistent identity: annotation1900955
display id: annotation1900955
version: 1
top level: BBa_B1007
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900955/range1900955/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900955/range1900955/1
type: Range
persistent identity: range1900955
display id: range1900955
version: 1
direction: direction/forward
top level: BBa_B1007
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1900958
display id: annotation1900958
version: 1
top level: BBa_B1007
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900958/range1900958/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900958/range1900958/1
type: Range
persistent identity: range1900958
display id: range1900958
version: 1
direction: direction/forward
top level: BBa_B1007
start: 32
end: 37
orientation: inline

modified thr
type: SequenceAnnotation
persistent identity: annotation1900956
display id: annotation1900956
version: 1
top level: BBa_B1007
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900956/range1900956/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900956/range1900956/1
type: Range
persistent identity: range1900956
display id: range1900956
version: 1
direction: direction/forward
top level: BBa_B1007
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1900957
display id: annotation1900957
version: 1
top level: BBa_B1007
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1007/annotation1900957/range1900957/1

https://synbiohub.org/public/igem/BBa_B1007/annotation1900957/range1900957/1
type: Range
persistent identity: range1900957
display id: range1900957
version: 1
direction: direction/reverse
top level: BBa_B1007
start: 4
end: 9
orientation: inline

BBa_I13539
type: ComponentDefinition
persistent identity: BBa I13539
display id: BBa_I13539
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Two promoter screening plasmid v1.2
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13453
component: BBa_I0500
component: BBa_B0034
component: BBa_E0040
component: BBa_B0010
component: BBa_I13457
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_I13457
sequence annotation: BBa_I13453
sequence annotation: BBa_E1010
sequence annotation: BBa_I0500
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13539_sequence/1

BBa_I13453
type: Component
persistent identity: component1679632
display id: component1679632
version: 1
top level: BBa_I13539
access: public
definition: BBa_I13453

BBa_I0500
type: Component
persistent identity: component1679579
display id: component1679579
version: 1
top level: BBa_I13539
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0034
type: Component
persistent identity: component1679604
display id: component1679604
version: 1
top level: BBa_I13539
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1679639
display id: component1679639
version: 1
top level: BBa_I13539
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1679644
display id: component1679644
version: 1
top level: BBa_I13539
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13457
type: Component
persistent identity: component1679599
display id: component1679599
version: 1
top level: BBa_I13539
access: public
definition: https://synbiohub.org/public/igem/BBa_I13457/1

BBa_E1010
type: Component
persistent identity: component1679611
display id: component1679611
version: 1
top level: BBa_I13539
access: public
definition: mRFP1

BBa_B0010
type: Component
persistent identity: component1679617
display id: component1679617
version: 1
top level: BBa_I13539
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1679637
display id: component1679637
version: 1
top level: BBa_I13539
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1679627
display id: component1679627
version: 1
top level: BBa_I13539
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component1679654
display id: component1679654
version: 1
top level: BBa_I13539
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1679637
display id: annotation1679637
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679637/range1679637/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13539/annotation1679637/range1679637/1
type: Range
persistent identity: range1679637
display id: range1679637
version: 1
direction: direction/forward
top level: BBa_I13539
start: 2345
end: 2356
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1679644
display id: annotation1679644
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679644/range1679644/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13539/annotation1679644/range1679644/1
type: Range
persistent identity: range1679644
display id: range1679644
version: 1
direction: direction/forward
top level: BBa_I13539
start: 3091
end: 3170
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation1679599
display id: annotation1679599
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679599/range1679599/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13539/annotation1679599/range1679599/1
type: Range
persistent identity: range1679599
display id: range1679599
version: 1
direction: direction/forward
top level: BBa_I13539
start: 1219
end: 1329
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1679632
display id: annotation1679632
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679632/range1679632/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13539/annotation1679632/range1679632/1
type: Range
persistent identity: range1679632
display id: range1679632
version: 1
direction: direction/forward
top level: BBa_I13539
start: 2207
end: 2336
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1679617
display id: annotation1679617
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679617/range1679617/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13539/annotation1679617/range1679617/1
type: Range
persistent identity: range1679617
display id: range1679617
version: 1
direction: direction/forward
top level: BBa_I13539
start: 2070
end: 2149
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1679611
display id: annotation1679611
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679611/range1679611/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13539/annotation1679611/range1679611/1
type: Range
persistent identity: range1679611
display id: range1679611
version: 1
direction: direction/forward
top level: BBa_I13539
start: 1356
end: 2036
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1679579
display id: annotation1679579
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679579/range1679579/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13539/annotation1679579/range1679579/1
type: Range
persistent identity: range1679579
display id: range1679579
version: 1
direction: direction/forward
top level: BBa_I13539
start: 1
end: 1210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1679627
display id: annotation1679627
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679627/range1679627/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13539/annotation1679627/range1679627/1
type: Range
persistent identity: range1679627
display id: range1679627
version: 1
direction: direction/forward
top level: BBa_I13539
start: 2158
end: 2198
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1679639
display id: annotation1679639
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679639/range1679639/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13539/annotation1679639/range1679639/1
type: Range
persistent identity: range1679639
display id: range1679639
version: 1
direction: direction/forward
top level: BBa_I13539
start: 2363
end: 3082
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1679604
display id: annotation1679604
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679604/range1679604/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13539/annotation1679604/range1679604/1
type: Range
persistent identity: range1679604
display id: range1679604
version: 1
direction: direction/forward
top level: BBa_I13539
start: 1338
end: 1349
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1679654
display id: annotation1679654
version: 1
top level: BBa_I13539
location: https://synbiohub.org/public/igem/BBa_I13539/annotation1679654/range1679654/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13539/annotation1679654/range1679654/1
type: Range
persistent identity: range1679654
display id: range1679654
version: 1
direction: direction/forward
top level: BBa_I13539
start: 3179
end: 3219
orientation: inline

BBa_S03814
type: ComponentDefinition
persistent identity: BBa S03814
display id: BBa_S03814
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: S0100:J06602
creator: Kenichiro
created: 2007-10-14T11:00:00Z
modified: 2015-05-08T01:14:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 2188
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J06602
component: BBa_S0100
sequence annotation: BBa_J06602
sequence annotation: BBa_S0100
sequence: https://synbiohub.org/public/igem/BBa_S03814_sequence/1

BBa_J06602
type: Component
persistent identity: component2248920
display id: component2248920
version: 1
top level: BBa_S03814
access: public
definition: https://synbiohub.org/public/igem/BBa_J06602/1

BBa_S0100
type: Component
persistent identity: component2248914
display id: component2248914
version: 1
top level: BBa_S03814
access: public
definition: https://synbiohub.org/public/igem/BBa_S0100/1

BBa_J06602
type: SequenceAnnotation
persistent identity: annotation2248920
display id: annotation2248920
version: 1
top level: BBa_S03814
location: https://synbiohub.org/public/igem/BBa_S03814/annotation2248920/range2248920/1
component: BBa_J06602

https://synbiohub.org/public/igem/BBa_S03814/annotation2248920/range2248920/1
type: Range
persistent identity: range2248920
display id: range2248920
version: 1
direction: direction/reverse
top level: BBa_S03814
start: 1180
end: 1911
orientation: inline

BBa_S0100
type: SequenceAnnotation
persistent identity: annotation2248914
display id: annotation2248914
version: 1
top level: BBa_S03814
location: https://synbiohub.org/public/igem/BBa_S03814/annotation2248914/range2248914/1
component: BBa_S0100

https://synbiohub.org/public/igem/BBa_S03814/annotation2248914/range2248914/1
type: Range
persistent identity: range2248914
display id: range2248914
version: 1
direction: direction/reverse
top level: BBa_S03814
start: 1
end: 1171
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced yellow fluorescent protein derived from A. victoria GFP
creator: Caitlin Conboy and Jennifer Braff
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

BBa_I13202
type: ComponentDefinition
persistent identity: BBa I13202
display id: BBa_I13202
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: 3OC6HSL Sender Controlled by Lac Repressible Promoter
creator: Barry Canton
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:33Z
discontinued: false
dominant: true
experience: experience/None
group u list: _101_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: A lac repressible promoter driving production of an untagged LuxI enzyme which produces an AHL (3OC6HSL).
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0011
component: BBa_B0034
component: BBa_B0010
component: BBa_C0161
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_B0010
sequence annotation: BBa_C0161
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13202_sequence/1

BBa_R0011
type: Component
persistent identity: component1484926
display id: component1484926
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_B0034
type: Component
persistent identity: component1484935
display id: component1484935
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1484942
display id: component1484942
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0161
type: Component
persistent identity: component1484937
display id: component1484937
version: 1
top level: BBa_I13202
access: public
definition: luxI

BBa_B0012
type: Component
persistent identity: component1484952
display id: component1484952
version: 1
top level: BBa_I13202
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1484935
display id: annotation1484935
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484935/range1484935/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13202/annotation1484935/range1484935/1
type: Range
persistent identity: range1484935
display id: range1484935
version: 1
direction: direction/forward
top level: BBa_I13202
start: 64
end: 75
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1484926
display id: annotation1484926
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484926/range1484926/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13202/annotation1484926/range1484926/1
type: Range
persistent identity: range1484926
display id: range1484926
version: 1
direction: direction/forward
top level: BBa_I13202
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1484942
display id: annotation1484942
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484942/range1484942/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13202/annotation1484942/range1484942/1
type: Range
persistent identity: range1484942
display id: range1484942
version: 1
direction: direction/forward
top level: BBa_I13202
start: 675
end: 754
orientation: inline

BBa_C0161
type: SequenceAnnotation
persistent identity: annotation1484937
display id: annotation1484937
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484937/range1484937/1
component: BBa_C0161

https://synbiohub.org/public/igem/BBa_I13202/annotation1484937/range1484937/1
type: Range
persistent identity: range1484937
display id: range1484937
version: 1
direction: direction/forward
top level: BBa_I13202
start: 82
end: 666
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1484952
display id: annotation1484952
version: 1
top level: BBa_I13202
location: https://synbiohub.org/public/igem/BBa_I13202/annotation1484952/range1484952/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13202/annotation1484952/range1484952/1
type: Range
persistent identity: range1484952
display id: range1484952
version: 1
direction: direction/forward
top level: BBa_I13202
start: 763
end: 803
orientation: inline

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
creator: Todd Eckdahl
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Reverse version of RBS BBa_B0030
mutable notes: Repeats in oligos caused unusual products during cloning
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
direction: direction/reverse
top level: BBa_J44001
start: 1
end: 15
orientation: inline

P22 attP
type: ComponentDefinition
persistent identity: BBa I11033
display id: BBa_I11033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P22 ''attP''
creator: 2004 Boston University iGEM team
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: P22 phage attP site (P-O-P')
mutable provenance: gi|51236723:21158-21417
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I11033_sequence/1

OmpR
type: ComponentDefinition
persistent identity: BBa R0084
display id: BBa_R0084
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (OmpR, positive)
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
mutable notes: The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: F2 OmpR
sequence annotation: F1 OmpR
sequence annotation: -35
sequence annotation: F3 OmpR
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0084_sequence/1

F2 OmpR
type: SequenceAnnotation
persistent identity: annotation301309
display id: annotation301309
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
type: Range
persistent identity: range301309
display id: range301309
version: 1
direction: direction/forward
top level: OmpR
start: 31
end: 48
orientation: inline

F1 OmpR
type: SequenceAnnotation
persistent identity: annotation301308
display id: annotation301308
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
type: Range
persistent identity: range301308
display id: range301308
version: 1
direction: direction/forward
top level: OmpR
start: 11
end: 28
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301317
display id: annotation301317
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
type: Range
persistent identity: range301317
display id: range301317
version: 1
direction: direction/forward
top level: OmpR
start: 73
end: 78
orientation: inline

F3 OmpR
type: SequenceAnnotation
persistent identity: annotation301313
display id: annotation301313
version: 1
top level: OmpR
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
type: Range
persistent identity: range301313
display id: range301313
version: 1
direction: direction/forward
top level: OmpR
start: 51
end: 68
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301318
display id: annotation301318
version: 1
top level: OmpR
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1

https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
type: Range
persistent identity: range301318
display id: range301318
version: 1
direction: direction/forward
top level: OmpR
start: 88
end: 93
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa C0050
display id: BBa_C0050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage HK022 (+LVA?)
creator: Reshma Shetty
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.
mutable notes: References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. Derived from STHK022N . Response from John Little (Arizona) regarding the start of HK022 cI From: <jlittle@email.arizona.edu> Date: Tue Jan 21, 2003 4:39:21 PM US/Eastern To: "Drew Endy" <endy@MIT.EDU> Subject: RE: hk022 cI start (naive question)? Hello Drew and Michael, I seriously doubt that the extra 27 aa are part of CI. It doesn't make sense in terms of the biology, for sure. In any case, the protein we characterized starts where Carlson and Little stated; as I recall, oR3 partially overlaps the start of cI. I have a vague memory of some possibly interesting biology, having to do with multicopy plasmids. I don't recall the findings themselves. Anyway, one possible explanation was that this extra N-terminal addition was made (e.g. from the pRE promoter, or from a message that arose from transcription around the entire plasmid), making a protein with altered functions. We never followed it up. Good luck John -- Original Message -- Date: Sun, 19 Jan 2003 15:26:26 -0500 Subject: hk022 cI start (naive question)? Cc: elowitm@rockefeller.edu To: jlittle@u.arizona.edu From: Drew Endy <endy@MIT.EDU> Hi John, I'm sitting here with Michael Elowitz and we're working through the sequence for HK022 cI. We noticed that the annotation from NC_002166 includes an "extra" 81 base pairs upstream of what we thought of as the actual start. It looks like this extra …
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SsrA
sequence annotation: OR3 partial
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence annotation: BBa_C0050
sequence annotation: cI HK022
sequence: https://synbiohub.org/public/igem/BBa_C0050_sequence/1

SsrA
type: SequenceAnnotation
persistent identity: annotation1736
display id: annotation1736
version: 1
top level: cI HK022
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
type: Range
persistent identity: range1736
display id: range1736
version: 1
direction: direction/forward
top level: cI HK022
start: 706
end: 738
orientation: inline

OR3 partial
type: SequenceAnnotation
persistent identity: annotation1737
display id: annotation1737
version: 1
top level: cI HK022
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
type: Range
persistent identity: range1737
display id: range1737
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 8
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1734
display id: annotation1734
version: 1
top level: cI HK022
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
type: Range
persistent identity: range1734
display id: range1734
version: 1
direction: direction/forward
top level: cI HK022
start: 739
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213990
display id: annotation2213990
version: 1
top level: cI HK022
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1

https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
type: Range
persistent identity: range2213990
display id: range2213990
version: 1
direction: direction/forward
top level: cI HK022
start: 745
end: 769
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation7033
display id: annotation7033
version: 1
top level: cI HK022
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1

https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
type: Range
persistent identity: range7033
display id: range7033
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 744
orientation: inline

cI HK022
type: SequenceAnnotation
persistent identity: annotation1735
display id: annotation1735
version: 1
top level: cI HK022
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1

https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
type: Range
persistent identity: range1735
display id: range1735
version: 1
direction: direction/forward
top level: cI HK022
start: 1
end: 744
orientation: inline

RBS-GFP-Te
type: ComponentDefinition
persistent identity: BBa I763020
display id: BBa_I763020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS-GFP (+LVA Tag)-Terminators
creator: Alice Pasini
created: 2007-08-26T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part composed by RBS (B0034), GFP with LVA tag and terminators.
mutable notes: Intermediate
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_J04031
sequence annotation: BBa_B0034
sequence annotation: BBa_J04031
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I763020_sequence/1

BBa_B0012
type: Component
persistent identity: component1942528
display id: component1942528
version: 1
top level: RBS-GFP-Te
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1942526
display id: component1942526
version: 1
top level: RBS-GFP-Te
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1942520
display id: component1942520
version: 1
top level: RBS-GFP-Te
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_J04031
type: Component
persistent identity: component1942525
display id: component1942525
version: 1
top level: RBS-GFP-Te
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1942520
display id: annotation1942520
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942520/range1942520/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I763020/annotation1942520/range1942520/1
type: Range
persistent identity: range1942520
display id: range1942520
version: 1
direction: direction/reverse
top level: RBS-GFP-Te
start: 1
end: 12
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation1942525
display id: annotation1942525
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942525/range1942525/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_I763020/annotation1942525/range1942525/1
type: Range
persistent identity: range1942525
display id: range1942525
version: 1
direction: direction/reverse
top level: RBS-GFP-Te
start: 19
end: 777
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1942528
display id: annotation1942528
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942528/range1942528/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I763020/annotation1942528/range1942528/1
type: Range
persistent identity: range1942528
display id: range1942528
version: 1
direction: direction/forward
top level: RBS-GFP-Te
start: 874
end: 914
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1942526
display id: annotation1942526
version: 1
top level: RBS-GFP-Te
location: https://synbiohub.org/public/igem/BBa_I763020/annotation1942526/range1942526/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I763020/annotation1942526/range1942526/1
type: Range
persistent identity: range1942526
display id: range1942526
version: 1
direction: direction/forward
top level: RBS-GFP-Te
start: 786
end: 865
orientation: inline

BBa_I759001
type: ComponentDefinition
persistent identity: BBa I759001
display id: BBa_I759001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-spacer-N
creator: Josh Michener
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _115_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pTet-spacer-N
mutable notes: None
mutable provenance: Caltech iGEM
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: N
sequence annotation: pTet
sequence annotation: spacer
sequence: https://synbiohub.org/public/igem/BBa_I759001_sequence/1

N
type: SequenceAnnotation
persistent identity: annotation1958470
display id: annotation1958470
version: 1
top level: BBa_I759001
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I759001/annotation1958470/range1958470/1

https://synbiohub.org/public/igem/BBa_I759001/annotation1958470/range1958470/1
type: Range
persistent identity: range1958470
display id: range1958470
version: 1
direction: direction/forward
top level: BBa_I759001
start: 113
end: 513
orientation: inline

pTet
type: SequenceAnnotation
persistent identity: annotation1958468
display id: annotation1958468
version: 1
top level: BBa_I759001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I759001/annotation1958468/range1958468/1

https://synbiohub.org/public/igem/BBa_I759001/annotation1958468/range1958468/1
type: Range
persistent identity: range1958468
display id: range1958468
version: 1
direction: direction/forward
top level: BBa_I759001
start: 1
end: 54
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1958469
display id: annotation1958469
version: 1
top level: BBa_I759001
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I759001/annotation1958469/range1958469/1

https://synbiohub.org/public/igem/BBa_I759001/annotation1958469/range1958469/1
type: Range
persistent identity: range1958469
display id: range1958469
version: 1
direction: direction/forward
top level: BBa_I759001
start: 55
end: 112
orientation: inline

BBa_I13452
type: ComponentDefinition
persistent identity: BBa I13452
display id: BBa_I13452
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Adds another BioBrick site
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains both a BbsI and a BstXI restriction site. When cut with both enzymes, it leaves an EcoRI and a PstI site.
mutable notes: 70 bp from Eco to Spe or Xba to Pst, to allow for gel extraction. The middle portion is largely noise, intended as filler to raise the size of the part.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Future Eco site
sequence annotation: Future Pst site
sequence annotation: BstXI site
sequence annotation: BbsI site
sequence: https://synbiohub.org/public/igem/BBa_I13452_sequence/1

Future Eco site
type: SequenceAnnotation
persistent identity: annotation1891619
display id: annotation1891619
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891619/range1891619/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891619/range1891619/1
type: Range
persistent identity: range1891619
display id: range1891619
version: 1
direction: direction/forward
top level: BBa_I13452
start: 1
end: 6
orientation: inline

Future Pst site
type: SequenceAnnotation
persistent identity: annotation1891622
display id: annotation1891622
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891622/range1891622/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891622/range1891622/1
type: Range
persistent identity: range1891622
display id: range1891622
version: 1
direction: direction/forward
top level: BBa_I13452
start: 44
end: 49
orientation: inline

BstXI site
type: SequenceAnnotation
persistent identity: annotation1891621
display id: annotation1891621
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891621/range1891621/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891621/range1891621/1
type: Range
persistent identity: range1891621
display id: range1891621
version: 1
direction: direction/forward
top level: BBa_I13452
start: 41
end: 52
orientation: inline

BbsI site
type: SequenceAnnotation
persistent identity: annotation1891620
display id: annotation1891620
version: 1
top level: BBa_I13452
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13452/annotation1891620/range1891620/1

https://synbiohub.org/public/igem/BBa_I13452/annotation1891620/range1891620/1
type: Range
persistent identity: range1891620
display id: range1891620
version: 1
direction: direction/forward
top level: BBa_I13452
start: 8
end: 13
orientation: inline

BBa_I718009
type: ComponentDefinition
persistent identity: BBa I718009
display id: BBa_I718009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-lox71-rbs-gfp-ter
creator: David Bikard
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _141_
m user id: 0
owner id: 1481
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Upstream construct of the synthetic multicellular bacteria
mutable notes: none
mutable provenance: none
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0040
component: BBa_B0010
component: BBa_J37028
component: BBa_B0012
component: BBa_E0040
component: BBa_B0030
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_J37028
sequence: https://synbiohub.org/public/igem/BBa_I718009_sequence/1

BBa_R0040
type: Component
persistent identity: component1956123
display id: component1956123
version: 1
top level: BBa_I718009
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0010
type: Component
persistent identity: component1956133
display id: component1956133
version: 1
top level: BBa_I718009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_J37028
type: Component
persistent identity: component1956127
display id: component1956127
version: 1
top level: BBa_I718009
access: public
definition: https://synbiohub.org/public/igem/BBa_J37028/1

BBa_B0012
type: Component
persistent identity: component1956135
display id: component1956135
version: 1
top level: BBa_I718009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1956132
display id: component1956132
version: 1
top level: BBa_I718009
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0030
type: Component
persistent identity: component1956129
display id: component1956129
version: 1
top level: BBa_I718009
access: public
definition: BBa_B0030

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956129
display id: annotation1956129
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956129/range1956129/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I718009/annotation1956129/range1956129/1
type: Range
persistent identity: range1956129
display id: range1956129
version: 1
direction: direction/forward
top level: BBa_I718009
start: 105
end: 119
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1956132
display id: annotation1956132
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956132/range1956132/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I718009/annotation1956132/range1956132/1
type: Range
persistent identity: range1956132
display id: range1956132
version: 1
direction: direction/reverse
top level: BBa_I718009
start: 126
end: 845
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956135
display id: annotation1956135
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956135/range1956135/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I718009/annotation1956135/range1956135/1
type: Range
persistent identity: range1956135
display id: range1956135
version: 1
direction: direction/forward
top level: BBa_I718009
start: 942
end: 982
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956133
display id: annotation1956133
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956133/range1956133/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I718009/annotation1956133/range1956133/1
type: Range
persistent identity: range1956133
display id: range1956133
version: 1
direction: direction/forward
top level: BBa_I718009
start: 854
end: 933
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1956123
display id: annotation1956123
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956123/range1956123/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I718009/annotation1956123/range1956123/1
type: Range
persistent identity: range1956123
display id: range1956123
version: 1
direction: direction/forward
top level: BBa_I718009
start: 1
end: 54
orientation: inline

BBa_J37028
type: SequenceAnnotation
persistent identity: annotation1956127
display id: annotation1956127
version: 1
top level: BBa_I718009
location: https://synbiohub.org/public/igem/BBa_I718009/annotation1956127/range1956127/1
component: BBa_J37028

https://synbiohub.org/public/igem/BBa_I718009/annotation1956127/range1956127/1
type: Range
persistent identity: range1956127
display id: range1956127
version: 1
direction: direction/reverse
top level: BBa_I718009
start: 63
end: 96
orientation: inline

BBa_K079050
type: ComponentDefinition
persistent identity: BBa K079050
display id: BBa_K079050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP reporter protein under the control of the J23100 constitutive promoter and LexA 2 operator
creator: Francesca Ceroni
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:08:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _185_
m user id: 0
owner id: 3530
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: ...
mutable notes: ...
mutable provenance: ..
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_J23100
component: BBa_B0034
component: BBa_K079040
component: BBa_J04031
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_K079040
sequence annotation: BBa_J23100
sequence annotation: BBa_J04031
sequence: https://synbiohub.org/public/igem/BBa_K079050_sequence/1

BBa_B0010
type: Component
persistent identity: component1988239
display id: component1988239
version: 1
top level: BBa_K079050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1988241
display id: component1988241
version: 1
top level: BBa_K079050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_J23100
type: Component
persistent identity: component1988230
display id: component1988230
version: 1
top level: BBa_K079050
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0034
type: Component
persistent identity: component1988233
display id: component1988233
version: 1
top level: BBa_K079050
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K079040
type: Component
persistent identity: component1988231
display id: component1988231
version: 1
top level: BBa_K079050
access: public
definition: https://synbiohub.org/public/igem/BBa_K079040/1

BBa_J04031
type: Component
persistent identity: component1988238
display id: component1988238
version: 1
top level: BBa_K079050
access: public
definition: https://synbiohub.org/public/igem/BBa_J04031/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1988241
display id: annotation1988241
version: 1
top level: BBa_K079050
location: https://synbiohub.org/public/igem/BBa_K079050/annotation1988241/range1988241/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K079050/annotation1988241/range1988241/1
type: Range
persistent identity: range1988241
display id: range1988241
version: 1
direction: direction/forward
top level: BBa_K079050
start: 941
end: 981
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1988233
display id: annotation1988233
version: 1
top level: BBa_K079050
location: https://synbiohub.org/public/igem/BBa_K079050/annotation1988233/range1988233/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K079050/annotation1988233/range1988233/1
type: Range
persistent identity: range1988233
display id: range1988233
version: 1
direction: direction/reverse
top level: BBa_K079050
start: 68
end: 79
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1988239
display id: annotation1988239
version: 1
top level: BBa_K079050
location: https://synbiohub.org/public/igem/BBa_K079050/annotation1988239/range1988239/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K079050/annotation1988239/range1988239/1
type: Range
persistent identity: range1988239
display id: range1988239
version: 1
direction: direction/forward
top level: BBa_K079050
start: 853
end: 932
orientation: inline

BBa_K079040
type: SequenceAnnotation
persistent identity: annotation1988231
display id: annotation1988231
version: 1
top level: BBa_K079050
location: https://synbiohub.org/public/igem/BBa_K079050/annotation1988231/range1988231/1
component: BBa_K079040

https://synbiohub.org/public/igem/BBa_K079050/annotation1988231/range1988231/1
type: Range
persistent identity: range1988231
display id: range1988231
version: 1
direction: direction/reverse
top level: BBa_K079050
start: 44
end: 59
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1988230
display id: annotation1988230
version: 1
top level: BBa_K079050
location: https://synbiohub.org/public/igem/BBa_K079050/annotation1988230/range1988230/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K079050/annotation1988230/range1988230/1
type: Range
persistent identity: range1988230
display id: range1988230
version: 1
direction: direction/reverse
top level: BBa_K079050
start: 1
end: 35
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation1988238
display id: annotation1988238
version: 1
top level: BBa_K079050
location: https://synbiohub.org/public/igem/BBa_K079050/annotation1988238/range1988238/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_K079050/annotation1988238/range1988238/1
type: Range
persistent identity: range1988238
display id: range1988238
version: 1
direction: direction/reverse
top level: BBa_K079050
start: 86
end: 844
orientation: inline

BBa_S03859
type: ComponentDefinition
persistent identity: BBa S03859
display id: BBa_S03859
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015:I718016
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 1568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_I718016
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_I718016
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S03859_sequence/1

BBa_B0012
type: Component
persistent identity: component1956471
display id: component1956471
version: 1
top level: BBa_S03859
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I718016
type: Component
persistent identity: component1956475
display id: component1956475
version: 1
top level: BBa_S03859
access: public
definition: https://synbiohub.org/public/igem/BBa_I718016/1

BBa_B0010
type: Component
persistent identity: component1956469
display id: component1956469
version: 1
top level: BBa_S03859
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956469
display id: annotation1956469
version: 1
top level: BBa_S03859
location: https://synbiohub.org/public/igem/BBa_S03859/annotation1956469/range1956469/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03859/annotation1956469/range1956469/1
type: Range
persistent identity: range1956469
display id: range1956469
version: 1
direction: direction/forward
top level: BBa_S03859
start: 1
end: 80
orientation: inline

BBa_I718016
type: SequenceAnnotation
persistent identity: annotation1956475
display id: annotation1956475
version: 1
top level: BBa_S03859
location: https://synbiohub.org/public/igem/BBa_S03859/annotation1956475/range1956475/1
component: BBa_I718016

https://synbiohub.org/public/igem/BBa_S03859/annotation1956475/range1956475/1
type: Range
persistent identity: range1956475
display id: range1956475
version: 1
direction: direction/reverse
top level: BBa_S03859
start: 138
end: 171
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956471
display id: annotation1956471
version: 1
top level: BBa_S03859
location: https://synbiohub.org/public/igem/BBa_S03859/annotation1956471/range1956471/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03859/annotation1956471/range1956471/1
type: Range
persistent identity: range1956471
display id: range1956471
version: 1
direction: direction/forward
top level: BBa_S03859
start: 89
end: 129
orientation: inline

BBa_I732094
type: ComponentDefinition
persistent identity: BBa I732094
display id: BBa_I732094
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter Activity Reporter (LacZ-alpha and GFP-AAV)
creator: Zhan Jian
created: 2007-09-19T11:00:00Z
modified: 2015-08-31T04:07:57Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1572
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: To be later edited.
mutable notes: To be later edited.
mutable provenance: To be later edited.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_I732007
component: BBa_I732006
component: BBa_E0044
sequence annotation: BBa_E0044
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I732006
sequence annotation: BBa_I732007
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732094_sequence/1

BBa_B0034
type: Component
persistent identity: component1944359
display id: component1944359
version: 1
top level: BBa_I732094
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1944361
display id: component1944361
version: 1
top level: BBa_I732094
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1944353
display id: component1944353
version: 1
top level: BBa_I732094
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1944363
display id: component1944363
version: 1
top level: BBa_I732094
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I732007
type: Component
persistent identity: component1944351
display id: component1944351
version: 1
top level: BBa_I732094
access: public
definition: Adapter

BBa_I732006
type: Component
persistent identity: component1944357
display id: component1944357
version: 1
top level: BBa_I732094
access: public
definition: lacZ-alpha

BBa_E0044
type: Component
persistent identity: component1944360
display id: component1944360
version: 1
top level: BBa_I732094
access: public
definition: https://synbiohub.org/public/igem/BBa_E0044/1

BBa_E0044
type: SequenceAnnotation
persistent identity: annotation1944360
display id: annotation1944360
version: 1
top level: BBa_I732094
location: https://synbiohub.org/public/igem/BBa_I732094/annotation1944360/range1944360/1
component: BBa_E0044

https://synbiohub.org/public/igem/BBa_I732094/annotation1944360/range1944360/1
type: Range
persistent identity: range1944360
display id: range1944360
version: 1
direction: direction/reverse
top level: BBa_I732094
start: 306
end: 1064
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1944361
display id: annotation1944361
version: 1
top level: BBa_I732094
location: https://synbiohub.org/public/igem/BBa_I732094/annotation1944361/range1944361/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732094/annotation1944361/range1944361/1
type: Range
persistent identity: range1944361
display id: range1944361
version: 1
direction: direction/forward
top level: BBa_I732094
start: 1073
end: 1152
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1944363
display id: annotation1944363
version: 1
top level: BBa_I732094
location: https://synbiohub.org/public/igem/BBa_I732094/annotation1944363/range1944363/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732094/annotation1944363/range1944363/1
type: Range
persistent identity: range1944363
display id: range1944363
version: 1
direction: direction/forward
top level: BBa_I732094
start: 1161
end: 1201
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1944357
display id: annotation1944357
version: 1
top level: BBa_I732094
location: https://synbiohub.org/public/igem/BBa_I732094/annotation1944357/range1944357/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732094/annotation1944357/range1944357/1
type: Range
persistent identity: range1944357
display id: range1944357
version: 1
direction: direction/reverse
top level: BBa_I732094
start: 46
end: 279
orientation: inline

BBa_I732007
type: SequenceAnnotation
persistent identity: annotation1944351
display id: annotation1944351
version: 1
top level: BBa_I732094
location: https://synbiohub.org/public/igem/BBa_I732094/annotation1944351/range1944351/1
component: BBa_I732007

https://synbiohub.org/public/igem/BBa_I732094/annotation1944351/range1944351/1
type: Range
persistent identity: range1944351
display id: range1944351
version: 1
direction: direction/reverse
top level: BBa_I732094
start: 1
end: 19
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944359
display id: annotation1944359
version: 1
top level: BBa_I732094
location: https://synbiohub.org/public/igem/BBa_I732094/annotation1944359/range1944359/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732094/annotation1944359/range1944359/1
type: Range
persistent identity: range1944359
display id: range1944359
version: 1
direction: direction/reverse
top level: BBa_I732094
start: 288
end: 299
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1944353
display id: annotation1944353
version: 1
top level: BBa_I732094
location: https://synbiohub.org/public/igem/BBa_I732094/annotation1944353/range1944353/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732094/annotation1944353/range1944353/1
type: Range
persistent identity: range1944353
display id: range1944353
version: 1
direction: direction/reverse
top level: BBa_I732094
start: 28
end: 39
orientation: inline

BBa_K206001
type: ComponentDefinition
persistent identity: BBa K206001
display id: BBa_K206001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pBAD weak
creator: Amelia Hardjasa
created: 2009-10-16T11:00:00Z
modified: 2015-05-08T01:11:23Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Weaker version of wild type pBAD ( I13453 ).
mutable notes: No special considerations
mutable provenance: Site-directed mutagenesis on I13453 with the following primers: Forward: TAATCTTATGGATAAAAAAGCTATGGCATAGC Reverse: GCGGATCCTACCTGACGCTTTTTATC
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: AraI1
sequence annotation: promoter
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206001_sequence/1

AraI1
type: SequenceAnnotation
persistent identity: annotation2049256
display id: annotation2049256
version: 1
top level: BBa_K206001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049256/range2049256/1
type: Range
persistent identity: range2049256
display id: range2049256
version: 1
direction: direction/forward
top level: BBa_K206001
start: 40
end: 57
orientation: inline

promoter
type: SequenceAnnotation
persistent identity: annotation2049255
display id: annotation2049255
version: 1
top level: BBa_K206001
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049255/range2049255/1
type: Range
persistent identity: range2049255
display id: range2049255
version: 1
direction: direction/forward
top level: BBa_K206001
start: 1
end: 131
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049257
display id: annotation2049257
version: 1
top level: BBa_K206001
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1

https://synbiohub.org/public/igem/BBa_K206001/annotation2049257/range2049257/1
type: Range
persistent identity: range2049257
display id: range2049257
version: 1
direction: direction/forward
top level: BBa_K206001
start: 61
end: 78
orientation: inline

BBa_P0151
type: ComponentDefinition
persistent identity: BBa P0151
display id: BBa_P0151
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cI (lambda) [S0165]
creator: Randy Rettberg
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Protein generator converting TIPS to the protein cI from lambda. Used as the input section for Quad Part Inverters Q01150 and Q01151.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0051
component: BBa_B0031
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_C0051
sequence annotation: BBa_B0031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0151_sequence/1

BBa_C0051
type: Component
persistent identity: component944513
display id: component944513
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0031
type: Component
persistent identity: component944503
display id: component944503
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0012
type: Component
persistent identity: component944529
display id: component944529
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component944519
display id: component944519
version: 1
top level: BBa_P0151
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation944513
display id: annotation944513
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
type: Range
persistent identity: range944513
display id: range944513
version: 1
direction: direction/forward
top level: BBa_P0151
start: 21
end: 770
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944503
display id: annotation944503
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
type: Range
persistent identity: range944503
display id: range944503
version: 1
direction: direction/forward
top level: BBa_P0151
start: 1
end: 14
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944519
display id: annotation944519
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
type: Range
persistent identity: range944519
display id: range944519
version: 1
direction: direction/forward
top level: BBa_P0151
start: 804
end: 883
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944529
display id: annotation944529
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
type: Range
persistent identity: range944529
display id: range944529
version: 1
direction: direction/forward
top level: BBa_P0151
start: 892
end: 932
orientation: inline

BBa_E0615
type: ComponentDefinition
persistent identity: BBa E0615
display id: BBa_E0615
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I0500.Q04530.E0430
creator: cconboy
created: 2004-05-13T11:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I0500
component: BBa_E0030
component: BBa_R0053
component: BBa_B0012
component: BBa_C0053
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0053
sequence annotation: BBa_E0030
sequence annotation: BBa_B0012
sequence annotation: BBa_I0500
sequence annotation: BBa_R0053
sequence: https://synbiohub.org/public/igem/BBa_E0615_sequence/1

BBa_B0034
type: Component
persistent identity: component965007
display id: component965007
version: 1
top level: BBa_E0615
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component964943
display id: component964943
version: 1
top level: BBa_E0615
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_E0030
type: Component
persistent identity: component965009
display id: component965009
version: 1
top level: BBa_E0615
access: public
definition: eyfp

BBa_R0053
type: Component
persistent identity: component964997
display id: component964997
version: 1
top level: BBa_E0615
access: public
definition: https://synbiohub.org/public/igem/BBa_R0053/1

BBa_B0012
type: Component
persistent identity: component964978
display id: component964978
version: 1
top level: BBa_E0615
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0053
type: Component
persistent identity: component964960
display id: component964960
version: 1
top level: BBa_E0615
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_B0034
type: Component
persistent identity: component964948
display id: component964948
version: 1
top level: BBa_E0615
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component964968
display id: component964968
version: 1
top level: BBa_E0615
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component965024
display id: component965024
version: 1
top level: BBa_E0615
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component965014
display id: component965014
version: 1
top level: BBa_E0615
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation965014
display id: annotation965014
version: 1
top level: BBa_E0615
location: https://synbiohub.org/public/igem/BBa_E0615/annotation965014/range965014/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0615/annotation965014/range965014/1
type: Range
persistent identity: range965014
display id: range965014
version: 1
direction: direction/forward
top level: BBa_E0615
start: 2905
end: 2984
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation964948
display id: annotation964948
version: 1
top level: BBa_E0615
location: https://synbiohub.org/public/igem/BBa_E0615/annotation964948/range964948/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0615/annotation964948/range964948/1
type: Range
persistent identity: range964948
display id: range964948
version: 1
direction: direction/forward
top level: BBa_E0615
start: 1219
end: 1230
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation965007
display id: annotation965007
version: 1
top level: BBa_E0615
location: https://synbiohub.org/public/igem/BBa_E0615/annotation965007/range965007/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0615/annotation965007/range965007/1
type: Range
persistent identity: range965007
display id: range965007
version: 1
direction: direction/forward
top level: BBa_E0615
start: 2156
end: 2167
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation964960
display id: annotation964960
version: 1
top level: BBa_E0615
location: https://synbiohub.org/public/igem/BBa_E0615/annotation964960/range964960/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_E0615/annotation964960/range964960/1
type: Range
persistent identity: range964960
display id: range964960
version: 1
direction: direction/forward
top level: BBa_E0615
start: 1237
end: 1923
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation965009
display id: annotation965009
version: 1
top level: BBa_E0615
location: https://synbiohub.org/public/igem/BBa_E0615/annotation965009/range965009/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_E0615/annotation965009/range965009/1
type: Range
persistent identity: range965009
display id: range965009
version: 1
direction: direction/forward
top level: BBa_E0615
start: 2174
end: 2896
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation964968
display id: annotation964968
version: 1
top level: BBa_E0615
location: https://synbiohub.org/public/igem/BBa_E0615/annotation964968/range964968/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0615/annotation964968/range964968/1
type: Range
persistent identity: range964968
display id: range964968
version: 1
direction: direction/forward
top level: BBa_E0615
start: 1957
end: 2036
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation964978
display id: annotation964978
version: 1
top level: BBa_E0615
location: https://synbiohub.org/public/igem/BBa_E0615/annotation964978/range964978/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0615/annotation964978/range964978/1
type: Range
persistent identity: range964978
display id: range964978
version: 1
direction: direction/forward
top level: BBa_E0615
start: 2045
end: 2085
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation964943
display id: annotation964943
version: 1
top level: BBa_E0615
location: https://synbiohub.org/public/igem/BBa_E0615/annotation964943/range964943/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_E0615/annotation964943/range964943/1
type: Range
persistent identity: range964943
display id: range964943
version: 1
direction: direction/forward
top level: BBa_E0615
start: 1
end: 1210
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation965024
display id: annotation965024
version: 1
top level: BBa_E0615
location: https://synbiohub.org/public/igem/BBa_E0615/annotation965024/range965024/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0615/annotation965024/range965024/1
type: Range
persistent identity: range965024
display id: range965024
version: 1
direction: direction/forward
top level: BBa_E0615
start: 2993
end: 3033
orientation: inline

BBa_R0053
type: SequenceAnnotation
persistent identity: annotation964997
display id: annotation964997
version: 1
top level: BBa_E0615
location: https://synbiohub.org/public/igem/BBa_E0615/annotation964997/range964997/1
component: BBa_R0053

https://synbiohub.org/public/igem/BBa_E0615/annotation964997/range964997/1
type: Range
persistent identity: range964997
display id: range964997
version: 1
direction: direction/forward
top level: BBa_E0615
start: 2094
end: 2147
orientation: inline

PT7-E
type: ComponentDefinition
persistent identity: BBa I716107
display id: BBa_I716107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter (Mutant E)
creator: David Tulga
created: 2007-06-08T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _110_
m user id: 0
owner id: 1694
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the T7 Promoter which is activated when the T7 RNA Polymerase is produced. It provides very good PoPS (Polymerases Per Second) output, as well as it is highly orthogonal to E. Coli's natural transcription system. This one of the moderately active (Mutant E) variations we are using.
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: This part was generated synthetically.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: T7 Promoter E
sequence: https://synbiohub.org/public/igem/BBa_I716107_sequence/1

T7 Promoter E
type: SequenceAnnotation
persistent identity: annotation1954611
display id: annotation1954611
version: 1
top level: PT7-E
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716107/annotation1954611/range1954611/1

https://synbiohub.org/public/igem/BBa_I716107/annotation1954611/range1954611/1
type: Range
persistent identity: range1954611
display id: range1954611
version: 1
direction: direction/forward
top level: PT7-E
start: 1
end: 29
orientation: inline

penI
type: ComponentDefinition
persistent identity: BBa C0074
display id: BBa_C0074
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: penI repressor from Bacillus licheniformis (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Since penI is being ported from Bacillus Licheniformis, the sequence was changed within the coding region to reflect codon usage in E.Coli K12. Codons were mapped to the codon of the same usage rank in E.Coli.
mutable provenance: bacillus licheniformis
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: penI
sequence: https://synbiohub.org/public/igem/BBa_C0074_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation306563
display id: annotation306563
version: 1
top level: penI
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1

https://synbiohub.org/public/igem/BBa_C0074/annotation306563/range306563/1
type: Range
persistent identity: range306563
display id: range306563
version: 1
direction: direction/forward
top level: penI
start: 385
end: 417
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214009
display id: annotation2214009
version: 1
top level: penI
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1

https://synbiohub.org/public/igem/BBa_C0074/annotation2214009/range2214009/1
type: Range
persistent identity: range2214009
display id: range2214009
version: 1
direction: direction/forward
top level: penI
start: 424
end: 448
orientation: inline

penI
type: SequenceAnnotation
persistent identity: annotation302638
display id: annotation302638
version: 1
top level: penI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1

https://synbiohub.org/public/igem/BBa_C0074/annotation302638/range302638/1
type: Range
persistent identity: range302638
display id: range302638
version: 1
direction: direction/forward
top level: penI
start: 1
end: 387
orientation: inline

BBa_I13907
type: ComponentDefinition
persistent identity: BBa I13907
display id: BBa_I13907
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Ring Oscillator Synchronization Device (lac)
creator: jasonk
created: 2004-12-28T12:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 2
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This device is meant to be coupled to the elowitz repressilator device to synchronize a population of oscillating cells.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0010
component: BBa_C0061
component: BBa_B0012
component: BBa_C0012
component: BBa_R0062
component: BBa_E0034
component: BBa_B0034
component: BBa_C0062
component: BBa_R0063
component: BBa_R0011
component: BBa_R0051
sequence annotation: BBa_B0012
sequence annotation: BBa_E0034
sequence annotation: BBa_B0010
sequence annotation: BBa_R0051
sequence annotation: BBa_R0011
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_R0063
sequence annotation: BBa_C0061
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_I13907_sequence/1

BBa_B0010
type: Component
persistent identity: component2238093
display id: component2238093
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0061
type: Component
persistent identity: component2238090
display id: component2238090
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0012
type: Component
persistent identity: component2238095
display id: component2238095
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0012
type: Component
persistent identity: component2238073
display id: component2238073
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_R0062
type: Component
persistent identity: component2238066
display id: component2238066
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_E0034
type: Component
persistent identity: component2238038
display id: component2238038
version: 1
top level: BBa_I13907
access: public
definition: EYFP

BBa_B0034
type: Component
persistent identity: component2238071
display id: component2238071
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2238088
display id: component2238088
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0062
type: Component
persistent identity: component2238055
display id: component2238055
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_R0063
type: Component
persistent identity: component2238046
display id: component2238046
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_R0063/1

BBa_B0010
type: Component
persistent identity: component2238059
display id: component2238059
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component2238061
display id: component2238061
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component2238078
display id: component2238078
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: Component
persistent identity: component2238041
display id: component2238041
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component2238039
display id: component2238039
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component2238076
display id: component2238076
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component2238035
display id: component2238035
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component2238052
display id: component2238052
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0011
type: Component
persistent identity: component2238082
display id: component2238082
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_R0051
type: Component
persistent identity: component2238030
display id: component2238030
version: 1
top level: BBa_I13907
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2238078
display id: annotation2238078
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238078/range2238078/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13907/annotation2238078/range2238078/1
type: Range
persistent identity: range2238078
display id: range2238078
version: 1
direction: direction/forward
top level: BBa_I13907
start: 3416
end: 3456
orientation: inline

BBa_E0034
type: SequenceAnnotation
persistent identity: annotation2238038
display id: annotation2238038
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238038/range2238038/1
component: BBa_E0034

https://synbiohub.org/public/igem/BBa_I13907/annotation2238038/range2238038/1
type: Range
persistent identity: range2238038
display id: range2238038
version: 1
direction: direction/reverse
top level: BBa_I13907
start: 76
end: 837
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2238095
display id: annotation2238095
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238095/range2238095/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13907/annotation2238095/range2238095/1
type: Range
persistent identity: range2238095
display id: range2238095
version: 1
direction: direction/forward
top level: BBa_I13907
start: 4285
end: 4325
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2238093
display id: annotation2238093
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238093/range2238093/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13907/annotation2238093/range2238093/1
type: Range
persistent identity: range2238093
display id: range2238093
version: 1
direction: direction/forward
top level: BBa_I13907
start: 4197
end: 4276
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2238061
display id: annotation2238061
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238061/range2238061/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13907/annotation2238061/range2238061/1
type: Range
persistent identity: range2238061
display id: range2238061
version: 1
direction: direction/forward
top level: BBa_I13907
start: 2037
end: 2077
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2238030
display id: annotation2238030
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238030/range2238030/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I13907/annotation2238030/range2238030/1
type: Range
persistent identity: range2238030
display id: range2238030
version: 1
direction: direction/forward
top level: BBa_I13907
start: 1
end: 49
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2238082
display id: annotation2238082
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238082/range2238082/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13907/annotation2238082/range2238082/1
type: Range
persistent identity: range2238082
display id: range2238082
version: 1
direction: direction/forward
top level: BBa_I13907
start: 3465
end: 3518
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2238039
display id: annotation2238039
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238039/range2238039/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13907/annotation2238039/range2238039/1
type: Range
persistent identity: range2238039
display id: range2238039
version: 1
direction: direction/forward
top level: BBa_I13907
start: 846
end: 925
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2238066
display id: annotation2238066
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238066/range2238066/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13907/annotation2238066/range2238066/1
type: Range
persistent identity: range2238066
display id: range2238066
version: 1
direction: direction/forward
top level: BBa_I13907
start: 2086
end: 2140
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2238035
display id: annotation2238035
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238035/range2238035/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13907/annotation2238035/range2238035/1
type: Range
persistent identity: range2238035
display id: range2238035
version: 1
direction: direction/reverse
top level: BBa_I13907
start: 58
end: 69
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2238071
display id: annotation2238071
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238071/range2238071/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13907/annotation2238071/range2238071/1
type: Range
persistent identity: range2238071
display id: range2238071
version: 1
direction: direction/reverse
top level: BBa_I13907
start: 2149
end: 2160
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2238052
display id: annotation2238052
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238052/range2238052/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13907/annotation2238052/range2238052/1
type: Range
persistent identity: range2238052
display id: range2238052
version: 1
direction: direction/reverse
top level: BBa_I13907
start: 1142
end: 1153
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2238088
display id: annotation2238088
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238088/range2238088/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13907/annotation2238088/range2238088/1
type: Range
persistent identity: range2238088
display id: range2238088
version: 1
direction: direction/reverse
top level: BBa_I13907
start: 3528
end: 3539
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2238076
display id: annotation2238076
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238076/range2238076/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13907/annotation2238076/range2238076/1
type: Range
persistent identity: range2238076
display id: range2238076
version: 1
direction: direction/forward
top level: BBa_I13907
start: 3328
end: 3407
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2238055
display id: annotation2238055
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238055/range2238055/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13907/annotation2238055/range2238055/1
type: Range
persistent identity: range2238055
display id: range2238055
version: 1
direction: direction/forward
top level: BBa_I13907
start: 1160
end: 1915
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2238046
display id: annotation2238046
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238046/range2238046/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I13907/annotation2238046/range2238046/1
type: Range
persistent identity: range2238046
display id: range2238046
version: 1
direction: direction/forward
top level: BBa_I13907
start: 983
end: 1133
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2238059
display id: annotation2238059
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238059/range2238059/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13907/annotation2238059/range2238059/1
type: Range
persistent identity: range2238059
display id: range2238059
version: 1
direction: direction/forward
top level: BBa_I13907
start: 1949
end: 2028
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2238090
display id: annotation2238090
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238090/range2238090/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_I13907/annotation2238090/range2238090/1
type: Range
persistent identity: range2238090
display id: range2238090
version: 1
direction: direction/forward
top level: BBa_I13907
start: 3546
end: 4163
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2238073
display id: annotation2238073
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238073/range2238073/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_I13907/annotation2238073/range2238073/1
type: Range
persistent identity: range2238073
display id: range2238073
version: 1
direction: direction/forward
top level: BBa_I13907
start: 2167
end: 3294
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2238041
display id: annotation2238041
version: 1
top level: BBa_I13907
location: https://synbiohub.org/public/igem/BBa_I13907/annotation2238041/range2238041/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13907/annotation2238041/range2238041/1
type: Range
persistent identity: range2238041
display id: range2238041
version: 1
direction: direction/forward
top level: BBa_I13907
start: 934
end: 974
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa I15016
display id: BBa_I15016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0032.ECFP
creator: Jeff Tabor
created: 2005-04-14T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _5_
m user id: 0
owner id: 88
owning group id: 5
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part accepts POPS to produce ECFP under the medium RBS B0032
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0032
component: BBa_E0020
sequence annotation: BBa_E0020
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_I15016_sequence/1

BBa_B0032
type: Component
persistent identity: component1478405
display id: component1478405
version: 1
top level: ecfp
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_E0020
type: Component
persistent identity: component1478408
display id: component1478408
version: 1
top level: ecfp
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1478408
display id: annotation1478408
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15016/annotation1478408/range1478408/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I15016/annotation1478408/range1478408/1
type: Range
persistent identity: range1478408
display id: range1478408
version: 1
direction: direction/forward
top level: ecfp
start: 20
end: 742
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1478405
display id: annotation1478405
version: 1
top level: ecfp
location: https://synbiohub.org/public/igem/BBa_I15016/annotation1478405/range1478405/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_I15016/annotation1478405/range1478405/1
type: Range
persistent identity: range1478405
display id: range1478405
version: 1
direction: direction/forward
top level: ecfp
start: 1
end: 13
orientation: inline

lasR
type: ComponentDefinition
persistent identity: BBa C0179
display id: BBa_C0179
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasR activator from P. aeruginosa PAO1(no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0079 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0179_sequence/1

BBa_J33204
type: ComponentDefinition
persistent identity: BBa J33204
display id: BBa_J33204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: xylE reporter gene with rbs
creator: Chris French
created: 2006-10-16T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _63_
m user id: 0
owner id: 837
owning group id: 63
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part includes the xylE gene from the Pseudomonas putida TOL (naphthalene and xylene degradadative plasmid) pWW0. This gene encodes the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This is a useful reporter gene; colonies or broths expressing active XylE, in the presence of oxygen, will rapidly convert catechol, a cheap colourless substrate, to a bright yellow compound with an absorbance maximum around 377 nm. The part includes the native ribosome binding site, so simply needs to be added after a suitable promoter to act as a reporter. I have previously used this gene to generate whole cell biosensors for various heavy metals. Note that, unlike Xgal etc., catechol in solution is prone to spontaneous oxidation resulting in brown melanin-like polymeric products, so is not stable enough to incorporate into plates or growth media; it should be dripped onto colonies (I use 10 mM catechol in water for this) or added to liquid cultures at a final concentration of about 0.5 mM prior to assay. Note also that there is a SacI site at the very start of the part, when the prefix is included, so this can be used as a replacement vector to introduce PCR products with SacI-SpeI ends into pSB1A2 giving them full Biobrick prefixes and suffixes (but don't forget the G base before the SpeI site). This results in shorter non-complementary tails on PCR primers than using a full prefix or suffix. You can test for colonies that have lost xylE using catechol as described above.
mutable notes: Note that this sequence includes the native ribsomome binding site. Also, when the prefix is included, there is a SacI site at the start, which allows this part to be used as a vector for insertion of PCR products with SacI-SpeI ends into pSB1A2, replacing xylE, giving the full Biobrick prefixes and suffixes (but don't forget the G base before the SpeI site). This results in shorter non-complementary tails on PCR primers than using a full prefix or suffix. You can test for colonies that have lost xylE using catechol as described above.
mutable provenance: The template DNA was kindly supplied by Dr. Peter Williams of the University of Wales, Bangor. The primer design was based on Genbank sequence M64747 (GI:151718). The sequence reported here was confirmed by sequencing the Biobrick construct.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: rbs
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_J33204_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1903406
display id: annotation1903406
version: 1
top level: BBa_J33204
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_J33204/annotation1903406/range1903406/1

https://synbiohub.org/public/igem/BBa_J33204/annotation1903406/range1903406/1
type: Range
persistent identity: range1903406
display id: range1903406
version: 1
direction: direction/forward
top level: BBa_J33204
start: 14
end: 19
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1903407
display id: annotation1903407
version: 1
top level: BBa_J33204
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J33204/annotation1903407/range1903407/1

https://synbiohub.org/public/igem/BBa_J33204/annotation1903407/range1903407/1
type: Range
persistent identity: range1903407
display id: range1903407
version: 1
direction: direction/forward
top level: BBa_J33204
start: 27
end: 950
orientation: inline

BBa_J04500
type: ComponentDefinition
persistent identity: BBa J04500
display id: BBa_J04500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: IPTG inducible promoter with RBS
creator: Kristen DeCelle
created: 2005-06-08T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0010.B0034
mutable provenance: Davidson Synth-Aces
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_R0010
sequence: https://synbiohub.org/public/igem/BBa_J04500_sequence/1

BBa_B0034
type: Component
persistent identity: component1508159
display id: component1508159
version: 1
top level: BBa_J04500
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0010
type: Component
persistent identity: component1508149
display id: component1508149
version: 1
top level: BBa_J04500
access: public
definition: LacI

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1508159
display id: annotation1508159
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J04500/annotation1508159/range1508159/1
type: Range
persistent identity: range1508159
display id: range1508159
version: 1
direction: direction/forward
top level: BBa_J04500
start: 209
end: 220
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1508149
display id: annotation1508149
version: 1
top level: BBa_J04500
location: https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_J04500/annotation1508149/range1508149/1
type: Range
persistent identity: range1508149
display id: range1508149
version: 1
direction: direction/forward
top level: BBa_J04500
start: 1
end: 200
orientation: inline

BBa_S01009
type: ComponentDefinition
persistent identity: BBa S01009
display id: BBa_S01009
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0071.E0420
creator: Randy Rettberg
created: 2005-06-10T11:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from Jen 1 tube 78
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_C0071
component: BBa_B0034
component: BBa_E0020
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0071
sequence annotation: BBa_E0020
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S01009_sequence/1

BBa_B0012
type: Component
persistent identity: component1511466
display id: component1511466
version: 1
top level: BBa_S01009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0071
type: Component
persistent identity: component1511443
display id: component1511443
version: 1
top level: BBa_S01009
access: public
definition: rhlR

BBa_B0034
type: Component
persistent identity: component1511433
display id: component1511433
version: 1
top level: BBa_S01009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: Component
persistent identity: component1511451
display id: component1511451
version: 1
top level: BBa_S01009
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component1511456
display id: component1511456
version: 1
top level: BBa_S01009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1511449
display id: component1511449
version: 1
top level: BBa_S01009
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511449
display id: annotation1511449
version: 1
top level: BBa_S01009
location: https://synbiohub.org/public/igem/BBa_S01009/annotation1511449/range1511449/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01009/annotation1511449/range1511449/1
type: Range
persistent identity: range1511449
display id: range1511449
version: 1
direction: direction/forward
top level: BBa_S01009
start: 814
end: 825
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1511433
display id: annotation1511433
version: 1
top level: BBa_S01009
location: https://synbiohub.org/public/igem/BBa_S01009/annotation1511433/range1511433/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01009/annotation1511433/range1511433/1
type: Range
persistent identity: range1511433
display id: range1511433
version: 1
direction: direction/forward
top level: BBa_S01009
start: 1
end: 12
orientation: inline

BBa_C0071
type: SequenceAnnotation
persistent identity: annotation1511443
display id: annotation1511443
version: 1
top level: BBa_S01009
location: https://synbiohub.org/public/igem/BBa_S01009/annotation1511443/range1511443/1
component: BBa_C0071

https://synbiohub.org/public/igem/BBa_S01009/annotation1511443/range1511443/1
type: Range
persistent identity: range1511443
display id: range1511443
version: 1
direction: direction/forward
top level: BBa_S01009
start: 19
end: 780
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1511451
display id: annotation1511451
version: 1
top level: BBa_S01009
location: https://synbiohub.org/public/igem/BBa_S01009/annotation1511451/range1511451/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_S01009/annotation1511451/range1511451/1
type: Range
persistent identity: range1511451
display id: range1511451
version: 1
direction: direction/forward
top level: BBa_S01009
start: 832
end: 1554
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1511466
display id: annotation1511466
version: 1
top level: BBa_S01009
location: https://synbiohub.org/public/igem/BBa_S01009/annotation1511466/range1511466/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S01009/annotation1511466/range1511466/1
type: Range
persistent identity: range1511466
display id: range1511466
version: 1
direction: direction/forward
top level: BBa_S01009
start: 1651
end: 1691
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1511456
display id: annotation1511456
version: 1
top level: BBa_S01009
location: https://synbiohub.org/public/igem/BBa_S01009/annotation1511456/range1511456/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S01009/annotation1511456/range1511456/1
type: Range
persistent identity: range1511456
display id: range1511456
version: 1
direction: direction/forward
top level: BBa_S01009
start: 1563
end: 1642
orientation: inline

BBa_I13025
type: ComponentDefinition
persistent identity: BBa I13025
display id: BBa_I13025
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt QPI Intermediate
creator: jenmitch
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_C0072
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0072
sequence: https://synbiohub.org/public/igem/BBa_I13025_sequence/1

BBa_C0072
type: Component
persistent identity: component939424
display id: component939424
version: 1
top level: BBa_I13025
access: public
definition: mnt (s)

BBa_B0034
type: Component
persistent identity: component939411
display id: component939411
version: 1
top level: BBa_I13025
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939411
display id: annotation939411
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13025/annotation939411/range939411/1
type: Range
persistent identity: range939411
display id: range939411
version: 1
direction: direction/forward
top level: BBa_I13025
start: 1
end: 12
orientation: inline

BBa_C0072
type: SequenceAnnotation
persistent identity: annotation939424
display id: annotation939424
version: 1
top level: BBa_I13025
location: https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
component: BBa_C0072

https://synbiohub.org/public/igem/BBa_I13025/annotation939424/range939424/1
type: Range
persistent identity: range939424
display id: range939424
version: 1
direction: direction/forward
top level: BBa_I13025
start: 19
end: 306
orientation: inline

BBa_I716211
type: ComponentDefinition
persistent identity: BBa I716211
display id: BBa_I716211
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Barnase active peptide
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: true
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Barnase active peptide (no start codon)
mutable notes: PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716211 sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
mutable provenance: PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L) Product is pBca9145-I716211 sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716211_sequence/1

mRFP1
type: ComponentDefinition
persistent identity: BBa E2050
display id: BBa_E2050
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: derivative of mRFP1, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2016-01-25T02:35:54Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 4206
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP derivative. Ex548nm/Em562. yeast codon optimized.
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mCherry (BBa_E2060).
mutable provenance: Shaner et al, 2004. Nat Biotech (22):1567-1571
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GSGTA linker
sequence annotation: mOrange
sequence annotation: Help:Barcodes
sequence annotation: MATSG linker
sequence: https://synbiohub.org/public/igem/BBa_E2050_sequence/1

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431919
display id: annotation1431919
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431919/range1431919/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431919/range1431919/1
type: Range
persistent identity: range1431919
display id: range1431919
version: 1
direction: direction/forward
top level: mRFP1
start: 724
end: 738
orientation: inline

mOrange
type: SequenceAnnotation
persistent identity: annotation1431920
display id: annotation1431920
version: 1
top level: mRFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431920/range1431920/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431920/range1431920/1
type: Range
persistent identity: range1431920
display id: range1431920
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 738
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214024
display id: annotation2214024
version: 1
top level: mRFP1
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2050/annotation2214024/range2214024/1

https://synbiohub.org/public/igem/BBa_E2050/annotation2214024/range2214024/1
type: Range
persistent identity: range2214024
display id: range2214024
version: 1
direction: direction/forward
top level: mRFP1
start: 745
end: 769
orientation: inline

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431915
display id: annotation1431915
version: 1
top level: mRFP1
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2050/annotation1431915/range1431915/1

https://synbiohub.org/public/igem/BBa_E2050/annotation1431915/range1431915/1
type: Range
persistent identity: range1431915
display id: range1431915
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 15
orientation: inline

BBa_K118006
type: ComponentDefinition
persistent identity: BBa K118006
display id: BBa_K118006
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+crtB (phytoene synthase)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087) preceded by an E. coli synthetic ribosome binding site (rbs). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene.
mutable notes: No special considerations.
mutable provenance: rbs: Escherichia coli JM109 genomic DNA. crtB: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_K118002
sequence annotation: BBa_J15001
sequence annotation: BBa_K118002
sequence: https://synbiohub.org/public/igem/BBa_K118006_sequence/1

BBa_J15001
type: Component
persistent identity: component1970363
display id: component1970363
version: 1
top level: BBa_K118006
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118002
type: Component
persistent identity: component1970364
display id: component1970364
version: 1
top level: BBa_K118006
access: public
definition: BBa_K118002

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970363
display id: annotation1970363
version: 1
top level: BBa_K118006
location: https://synbiohub.org/public/igem/BBa_K118006/annotation1970363/range1970363/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118006/annotation1970363/range1970363/1
type: Range
persistent identity: range1970363
display id: range1970363
version: 1
direction: direction/reverse
top level: BBa_K118006
start: 1
end: 10
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation1970364
display id: annotation1970364
version: 1
top level: BBa_K118006
location: https://synbiohub.org/public/igem/BBa_K118006/annotation1970364/range1970364/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K118006/annotation1970364/range1970364/1
type: Range
persistent identity: range1970364
display id: range1970364
version: 1
direction: direction/reverse
top level: BBa_K118006
start: 17
end: 949
orientation: inline

BBa_B0017
type: ComponentDefinition
persistent identity: BBa B0017
display id: BBa_B0017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0010-B0010)
creator: Austin Che
created: 2004-01-08T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Double terminator with two copies of <bb_part>BBa_B0010</bb_part>
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0017_sequence/1

BBa_B0010
type: Component
persistent identity: component939337
display id: component939337
version: 1
top level: BBa_B0017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: Component
persistent identity: component939331
display id: component939331
version: 1
top level: BBa_B0017
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939337
display id: annotation939337
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
type: Range
persistent identity: range939337
display id: range939337
version: 1
direction: direction/forward
top level: BBa_B0017
start: 89
end: 168
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939331
display id: annotation939331
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
type: Range
persistent identity: range939331
display id: range939331
version: 1
direction: direction/forward
top level: BBa_B0017
start: 1
end: 80
orientation: inline

BBa_I11021
type: ComponentDefinition
persistent identity: BBa I11021
display id: BBa_I11021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: excisionase from E. coli phage lambda (removes prophage from host genome)
creator: mschomp
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Lambda excisionase coding region with AAV degradation tag and two stop codons
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: stop
sequence annotation: cI lambda
sequence annotation: AAV
sequence: https://synbiohub.org/public/igem/BBa_I11021_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214029
display id: annotation2214029
version: 1
top level: BBa_I11021
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_I11021/annotation2214029/range2214029/1

https://synbiohub.org/public/igem/BBa_I11021/annotation2214029/range2214029/1
type: Range
persistent identity: range2214029
display id: range2214029
version: 1
direction: direction/forward
top level: BBa_I11021
start: 256
end: 280
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1064104
display id: annotation1064104
version: 1
top level: BBa_I11021
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I11021/annotation1064104/range1064104/1

https://synbiohub.org/public/igem/BBa_I11021/annotation1064104/range1064104/1
type: Range
persistent identity: range1064104
display id: range1064104
version: 1
direction: direction/forward
top level: BBa_I11021
start: 250
end: 256
orientation: inline

cI lambda
type: SequenceAnnotation
persistent identity: annotation1064105
display id: annotation1064105
version: 1
top level: BBa_I11021
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I11021/annotation1064105/range1064105/1

https://synbiohub.org/public/igem/BBa_I11021/annotation1064105/range1064105/1
type: Range
persistent identity: range1064105
display id: range1064105
version: 1
direction: direction/forward
top level: BBa_I11021
start: 1
end: 212
orientation: inline

AAV
type: SequenceAnnotation
persistent identity: annotation1064103
display id: annotation1064103
version: 1
top level: BBa_I11021
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_I11021/annotation1064103/range1064103/1

https://synbiohub.org/public/igem/BBa_I11021/annotation1064103/range1064103/1
type: Range
persistent identity: range1064103
display id: range1064103
version: 1
direction: direction/forward
top level: BBa_I11021
start: 213
end: 249
orientation: inline

BBa_C0056
type: ComponentDefinition
persistent identity: BBa C0056
display id: BBa_C0056
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor from phage 434 (no LVA)
creator: Reshma Shetty
created: 2004-09-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: Removed LVA tag from BBa_C0052 via PCR. In pSB1AK3 currently.
mutable provenance: Removed LVA tag from BBa_C0052
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI 434
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_C0056_sequence/1

cI 434
type: SequenceAnnotation
persistent identity: annotation1063931
display id: annotation1063931
version: 1
top level: BBa_C0056
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1

https://synbiohub.org/public/igem/BBa_C0056/annotation1063931/range1063931/1
type: Range
persistent identity: range1063931
display id: range1063931
version: 1
direction: direction/forward
top level: BBa_C0056
start: 1
end: 636
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1063930
display id: annotation1063930
version: 1
top level: BBa_C0056
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1

https://synbiohub.org/public/igem/BBa_C0056/annotation1063930/range1063930/1
type: Range
persistent identity: range1063930
display id: range1063930
version: 1
direction: direction/forward
top level: BBa_C0056
start: 631
end: 636
orientation: inline

BBa_I732801
type: ComponentDefinition
persistent identity: BBa I732801
display id: BBa_I732801
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LRLa (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I732101
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_I732101
sequence: https://synbiohub.org/public/igem/BBa_I732801_sequence/1

BBa_I732101
type: Component
persistent identity: component1946166
display id: component1946166
version: 1
top level: BBa_I732801
access: public
definition: LRLa

BBa_B0034
type: Component
persistent identity: component1946165
display id: component1946165
version: 1
top level: BBa_I732801
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946165
display id: annotation1946165
version: 1
top level: BBa_I732801
location: https://synbiohub.org/public/igem/BBa_I732801/annotation1946165/range1946165/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732801/annotation1946165/range1946165/1
type: Range
persistent identity: range1946165
display id: range1946165
version: 1
direction: direction/reverse
top level: BBa_I732801
start: 1
end: 12
orientation: inline

BBa_I732101
type: SequenceAnnotation
persistent identity: annotation1946166
display id: annotation1946166
version: 1
top level: BBa_I732801
location: https://synbiohub.org/public/igem/BBa_I732801/annotation1946166/range1946166/1
component: BBa_I732101

https://synbiohub.org/public/igem/BBa_I732801/annotation1946166/range1946166/1
type: Range
persistent identity: range1946166
display id: range1946166
version: 1
direction: direction/reverse
top level: BBa_I732801
start: 19
end: 1104
orientation: inline

BBa_K137058
type: ComponentDefinition
persistent identity: BBa K137058
display id: BBa_K137058
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP fimE switch with 250 bp tetR promoter
creator: Allen Lin
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: A promoter facing upstream is flanked by two FimE binding sites. GFP is downstream to this fimE switch. Active FimE will flip the promoter such that GFP is expresssed.
mutable notes: None.
mutable provenance: Previously constructed Biobrick parts.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K137008
component: BBa_K137047
component: BBa_B0012
component: BBa_E0040
component: BBa_K137010
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_K137010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_K137008
sequence annotation: BBa_K137047
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K137058_sequence/1

BBa_K137008
type: Component
persistent identity: component1969295
display id: component1969295
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_K137008/1

BBa_K137047
type: Component
persistent identity: component1969292
display id: component1969292
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_K137047/1

BBa_B0012
type: Component
persistent identity: component1969302
display id: component1969302
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1969299
display id: component1969299
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K137010
type: Component
persistent identity: component1969290
display id: component1969290
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_K137010/1

BBa_B0010
type: Component
persistent identity: component1969300
display id: component1969300
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1969297
display id: component1969297
version: 1
top level: BBa_K137058
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation1969290
display id: annotation1969290
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969290/range1969290/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_K137058/annotation1969290/range1969290/1
type: Range
persistent identity: range1969290
display id: range1969290
version: 1
direction: direction/reverse
top level: BBa_K137058
start: 1
end: 35
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1969302
display id: annotation1969302
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969302/range1969302/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137058/annotation1969302/range1969302/1
type: Range
persistent identity: range1969302
display id: range1969302
version: 1
direction: direction/forward
top level: BBa_K137058
start: 1179
end: 1219
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1969299
display id: annotation1969299
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969299/range1969299/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K137058/annotation1969299/range1969299/1
type: Range
persistent identity: range1969299
display id: range1969299
version: 1
direction: direction/reverse
top level: BBa_K137058
start: 363
end: 1082
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation1969295
display id: annotation1969295
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969295/range1969295/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_K137058/annotation1969295/range1969295/1
type: Range
persistent identity: range1969295
display id: range1969295
version: 1
direction: direction/reverse
top level: BBa_K137058
start: 302
end: 336
orientation: inline

BBa_K137047
type: SequenceAnnotation
persistent identity: annotation1969292
display id: annotation1969292
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969292/range1969292/1
component: BBa_K137047

https://synbiohub.org/public/igem/BBa_K137058/annotation1969292/range1969292/1
type: Range
persistent identity: range1969292
display id: range1969292
version: 1
direction: direction/reverse
top level: BBa_K137058
start: 44
end: 293
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1969300
display id: annotation1969300
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969300/range1969300/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137058/annotation1969300/range1969300/1
type: Range
persistent identity: range1969300
display id: range1969300
version: 1
direction: direction/forward
top level: BBa_K137058
start: 1091
end: 1170
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1969297
display id: annotation1969297
version: 1
top level: BBa_K137058
location: https://synbiohub.org/public/igem/BBa_K137058/annotation1969297/range1969297/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137058/annotation1969297/range1969297/1
type: Range
persistent identity: range1969297
display id: range1969297
version: 1
direction: direction/reverse
top level: BBa_K137058
start: 345
end: 356
orientation: inline

BBa_I13023
type: ComponentDefinition
persistent identity: BBa I13023
display id: BBa_I13023
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: QPI Test Construct Intermediate (Q01400.E0430)
creator: Barry Canton
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_R0040
component: BBa_C0040
component: BBa_E0030
component: BBa_B0010
component: BBa_B0031
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_B0031
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_I13023_sequence/1

BBa_B0012
type: Component
persistent identity: component962702
display id: component962702
version: 1
top level: BBa_I13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0040
type: Component
persistent identity: component962677
display id: component962677
version: 1
top level: BBa_I13023
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0040
type: Component
persistent identity: component962646
display id: component962646
version: 1
top level: BBa_I13023
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_E0030
type: Component
persistent identity: component962687
display id: component962687
version: 1
top level: BBa_I13023
access: public
definition: eyfp

BBa_B0012
type: Component
persistent identity: component962662
display id: component962662
version: 1
top level: BBa_I13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component962652
display id: component962652
version: 1
top level: BBa_I13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0031
type: Component
persistent identity: component962636
display id: component962636
version: 1
top level: BBa_I13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0010
type: Component
persistent identity: component962692
display id: component962692
version: 1
top level: BBa_I13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component962685
display id: component962685
version: 1
top level: BBa_I13023
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation962685
display id: annotation962685
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962685/range962685/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13023/annotation962685/range962685/1
type: Range
persistent identity: range962685
display id: range962685
version: 1
direction: direction/forward
top level: BBa_I13023
start: 913
end: 924
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation962636
display id: annotation962636
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962636/range962636/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I13023/annotation962636/range962636/1
type: Range
persistent identity: range962636
display id: range962636
version: 1
direction: direction/forward
top level: BBa_I13023
start: 1
end: 14
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation962677
display id: annotation962677
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962677/range962677/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13023/annotation962677/range962677/1
type: Range
persistent identity: range962677
display id: range962677
version: 1
direction: direction/forward
top level: BBa_I13023
start: 851
end: 904
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation962702
display id: annotation962702
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962702/range962702/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13023/annotation962702/range962702/1
type: Range
persistent identity: range962702
display id: range962702
version: 1
direction: direction/forward
top level: BBa_I13023
start: 1750
end: 1790
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation962652
display id: annotation962652
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962652/range962652/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13023/annotation962652/range962652/1
type: Range
persistent identity: range962652
display id: range962652
version: 1
direction: direction/forward
top level: BBa_I13023
start: 714
end: 793
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation962687
display id: annotation962687
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962687/range962687/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13023/annotation962687/range962687/1
type: Range
persistent identity: range962687
display id: range962687
version: 1
direction: direction/forward
top level: BBa_I13023
start: 931
end: 1653
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation962646
display id: annotation962646
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962646/range962646/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_I13023/annotation962646/range962646/1
type: Range
persistent identity: range962646
display id: range962646
version: 1
direction: direction/forward
top level: BBa_I13023
start: 21
end: 680
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation962662
display id: annotation962662
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962662/range962662/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13023/annotation962662/range962662/1
type: Range
persistent identity: range962662
display id: range962662
version: 1
direction: direction/forward
top level: BBa_I13023
start: 802
end: 842
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation962692
display id: annotation962692
version: 1
top level: BBa_I13023
location: https://synbiohub.org/public/igem/BBa_I13023/annotation962692/range962692/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13023/annotation962692/range962692/1
type: Range
persistent identity: range962692
display id: range962692
version: 1
direction: direction/forward
top level: BBa_I13023
start: 1662
end: 1741
orientation: inline

BBa_I759002
type: ComponentDefinition
persistent identity: BBa I759002
display id: BBa_I759002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet-spacer-cro
creator: Josh Michener
created: 2007-06-26T11:00:00Z
modified: 2015-08-31T04:08:07Z
discontinued: false
dominant: false
experience: experience/None
group u list: _115_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: pTet-spacer-cro
mutable notes: None
mutable provenance: Caltech iGEM
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
sequence annotation: cro gene
sequence annotation: spacer
sequence annotation: pTet
sequence: https://synbiohub.org/public/igem/BBa_I759002_sequence/1

cro gene
type: SequenceAnnotation
persistent identity: annotation1957528
display id: annotation1957528
version: 1
top level: BBa_I759002
role: SO:0000110
role: feature/dna
location: https://synbiohub.org/public/igem/BBa_I759002/annotation1957528/range1957528/1

https://synbiohub.org/public/igem/BBa_I759002/annotation1957528/range1957528/1
type: Range
persistent identity: range1957528
display id: range1957528
version: 1
direction: direction/forward
top level: BBa_I759002
start: 112
end: 312
orientation: inline

spacer
type: SequenceAnnotation
persistent identity: annotation1957529
display id: annotation1957529
version: 1
top level: BBa_I759002
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I759002/annotation1957529/range1957529/1

https://synbiohub.org/public/igem/BBa_I759002/annotation1957529/range1957529/1
type: Range
persistent identity: range1957529
display id: range1957529
version: 1
direction: direction/forward
top level: BBa_I759002
start: 55
end: 111
orientation: inline

pTet
type: SequenceAnnotation
persistent identity: annotation1957527
display id: annotation1957527
version: 1
top level: BBa_I759002
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I759002/annotation1957527/range1957527/1

https://synbiohub.org/public/igem/BBa_I759002/annotation1957527/range1957527/1
type: Range
persistent identity: range1957527
display id: range1957527
version: 1
direction: direction/forward
top level: BBa_I759002
start: 1
end: 54
orientation: inline

BBa_S03855
type: ComponentDefinition
persistent identity: BBa S03855
display id: BBa_S03855
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I718017:E0840
creator: Eimad Shotar
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
discontinued: false
dominant: true
group u list: _9_
m user id: 0
owner id: 1568
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0012
component: BBa_I718017
component: BBa_B0010
component: BBa_E0040
component: BBa_B0030
sequence annotation: BBa_B0012
sequence annotation: BBa_I718017
sequence annotation: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_S03855_sequence/1

BBa_B0012
type: Component
persistent identity: component1956431
display id: component1956431
version: 1
top level: BBa_S03855
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I718017
type: Component
persistent identity: component1956423
display id: component1956423
version: 1
top level: BBa_S03855
access: public
definition: lox71

BBa_B0010
type: Component
persistent identity: component1956429
display id: component1956429
version: 1
top level: BBa_S03855
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1956428
display id: component1956428
version: 1
top level: BBa_S03855
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0030
type: Component
persistent identity: component1956425
display id: component1956425
version: 1
top level: BBa_S03855
access: public
definition: BBa_B0030

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1956431
display id: annotation1956431
version: 1
top level: BBa_S03855
location: https://synbiohub.org/public/igem/BBa_S03855/annotation1956431/range1956431/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03855/annotation1956431/range1956431/1
type: Range
persistent identity: range1956431
display id: range1956431
version: 1
direction: direction/forward
top level: BBa_S03855
start: 880
end: 920
orientation: inline

BBa_I718017
type: SequenceAnnotation
persistent identity: annotation1956423
display id: annotation1956423
version: 1
top level: BBa_S03855
location: https://synbiohub.org/public/igem/BBa_S03855/annotation1956423/range1956423/1
component: BBa_I718017

https://synbiohub.org/public/igem/BBa_S03855/annotation1956423/range1956423/1
type: Range
persistent identity: range1956423
display id: range1956423
version: 1
direction: direction/reverse
top level: BBa_S03855
start: 1
end: 34
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1956429
display id: annotation1956429
version: 1
top level: BBa_S03855
location: https://synbiohub.org/public/igem/BBa_S03855/annotation1956429/range1956429/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03855/annotation1956429/range1956429/1
type: Range
persistent identity: range1956429
display id: range1956429
version: 1
direction: direction/forward
top level: BBa_S03855
start: 792
end: 871
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1956425
display id: annotation1956425
version: 1
top level: BBa_S03855
location: https://synbiohub.org/public/igem/BBa_S03855/annotation1956425/range1956425/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_S03855/annotation1956425/range1956425/1
type: Range
persistent identity: range1956425
display id: range1956425
version: 1
direction: direction/forward
top level: BBa_S03855
start: 43
end: 57
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1956428
display id: annotation1956428
version: 1
top level: BBa_S03855
location: https://synbiohub.org/public/igem/BBa_S03855/annotation1956428/range1956428/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S03855/annotation1956428/range1956428/1
type: Range
persistent identity: range1956428
display id: range1956428
version: 1
direction: direction/reverse
top level: BBa_S03855
start: 64
end: 783
orientation: inline

BBa_K081000
type: ComponentDefinition
persistent identity: BBa K081000
display id: BBa_K081000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Mux CH0
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This device is a PoPS in -> luxI out protein generator with Plux promoter downstream. It is a module of the following genetic multiplexer (mux): {|align="center" | |- | |} where: *PA, PS and PB are three promoters that can be ACTIVATED by the three generic elements A, S and B respectively; *GOI is the ''gene of interest'' that is the output of the system. In particular, '''K081000 is Channel 0'''. People who want to use the genetic mux have to assemble: *K081000 under the desired regulation for Channel 0 (for example the generic promoter PA); *K081001 under the desired regulation for Channel 1 (for example the generic promoter PB); *K081002 under the desired regulation for Selector (for example the generic promoter PS); *the desired protein generator downstream of K081000 and K081001 (for example the generic RBS-GOI-T generator);
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: luxI: T: Plux:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B1006
component: BBa_R0062
component: BBa_C0061
component: BBa_B0030
sequence annotation: BBa_C0061
sequence annotation: BBa_B1006
sequence annotation: BBa_B0030
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K081000_sequence/1

BBa_B1006
type: Component
persistent identity: component2249268
display id: component2249268
version: 1
top level: BBa_K081000
access: public
definition: BBa_B1006

BBa_R0062
type: Component
persistent identity: component2249270
display id: component2249270
version: 1
top level: BBa_K081000
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_C0061
type: Component
persistent identity: component2249261
display id: component2249261
version: 1
top level: BBa_K081000
access: public
definition: https://synbiohub.org/public/igem/BBa_C0061/1

BBa_B0030
type: Component
persistent identity: component2249258
display id: component2249258
version: 1
top level: BBa_K081000
access: public
definition: BBa_B0030

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2249261
display id: annotation2249261
version: 1
top level: BBa_K081000
location: https://synbiohub.org/public/igem/BBa_K081000/annotation2249261/range2249261/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K081000/annotation2249261/range2249261/1
type: Range
persistent identity: range2249261
display id: range2249261
version: 1
direction: direction/forward
top level: BBa_K081000
start: 22
end: 639
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2249268
display id: annotation2249268
version: 1
top level: BBa_K081000
location: https://synbiohub.org/public/igem/BBa_K081000/annotation2249268/range2249268/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081000/annotation2249268/range2249268/1
type: Range
persistent identity: range2249268
display id: range2249268
version: 1
direction: direction/reverse
top level: BBa_K081000
start: 673
end: 711
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2249258
display id: annotation2249258
version: 1
top level: BBa_K081000
location: https://synbiohub.org/public/igem/BBa_K081000/annotation2249258/range2249258/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081000/annotation2249258/range2249258/1
type: Range
persistent identity: range2249258
display id: range2249258
version: 1
direction: direction/forward
top level: BBa_K081000
start: 1
end: 15
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2249270
display id: annotation2249270
version: 1
top level: BBa_K081000
location: https://synbiohub.org/public/igem/BBa_K081000/annotation2249270/range2249270/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K081000/annotation2249270/range2249270/1
type: Range
persistent identity: range2249270
display id: range2249270
version: 1
direction: direction/forward
top level: BBa_K081000
start: 720
end: 774
orientation: inline

BBa_I714033
type: ComponentDefinition
persistent identity: BBa I714033
display id: BBa_I714033
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: crRNA-lock3-var1 (A new "lock" designed by our team)
creator: Mingzhi Qu
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:07:47Z
discontinued: false
dominant: false
experience: experience/None
group u list: _142_
m user id: 0
owner id: 2248
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: crRNA(I714033) and taRNA(I714034) whose structure resemble that of J23078 (lock3) and J23066 (key3) but with a different matching regions to minimize the possible interaction with the key3-lock3.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_I714033_sequence/1

COMT
type: ComponentDefinition
persistent identity: BBa I742107
display id: BBa_I742107
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: COMT
creator: sarah hollingshead
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: COMT coding sequence Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring) from Medicago sativa (alfalfa), synthesised by Geneart
mutable notes: Used cDNA as coding gene in Alfalfa genome contains introns.
mutable provenance: Alfalfa cDNA Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: COMT coding region
sequence: https://synbiohub.org/public/igem/BBa_I742107_sequence/1

COMT coding region
type: SequenceAnnotation
persistent identity: annotation1949817
display id: annotation1949817
version: 1
top level: COMT
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I742107/annotation1949817/range1949817/1

https://synbiohub.org/public/igem/BBa_I742107/annotation1949817/range1949817/1
type: Range
persistent identity: range1949817
display id: range1949817
version: 1
direction: direction/forward
top level: COMT
start: 1
end: 1101
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: double terminator (B0010-B0012)
creator: Reshma Shetty
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
direction: direction/forward
top level: BBa_B0015
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
direction: direction/forward
top level: BBa_B0015
start: 89
end: 129
orientation: inline

Adapter
type: ComponentDefinition
persistent identity: BBa I732007
display id: BBa_I732007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BglII + BamHI
creator: Zhan Jian
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BglII and BamHI restriction sites with some flanking
mutable notes: None
mutable provenance: http://openwetware.org/wiki/Silver:_Oligonucleotide_Inserts Oligonucleotide Inserts
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: BglII
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_I732007_sequence/1

BglII
type: SequenceAnnotation
persistent identity: annotation1936907
display id: annotation1936907
version: 1
top level: Adapter
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732007/annotation1936907/range1936907/1

https://synbiohub.org/public/igem/BBa_I732007/annotation1936907/range1936907/1
type: Range
persistent identity: range1936907
display id: range1936907
version: 1
direction: direction/forward
top level: Adapter
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1936908
display id: annotation1936908
version: 1
top level: Adapter
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I732007/annotation1936908/range1936908/1

https://synbiohub.org/public/igem/BBa_I732007/annotation1936908/range1936908/1
type: Range
persistent identity: range1936908
display id: range1936908
version: 1
direction: direction/forward
top level: Adapter
start: 12
end: 17
orientation: inline

BBa_B1003
type: ComponentDefinition
persistent identity: BBa B1003
display id: BBa_B1003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, small, %T~=80)
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artifical terminator, estimated %T~=80 6bp stem, 4nt loop Bidirectional, estimated reverse %T=40
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 5 residues.
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: stem loop
sequence annotation: B1003
sequence: https://synbiohub.org/public/igem/BBa_B1003_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1898419
display id: annotation1898419
version: 1
top level: BBa_B1003
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898419/range1898419/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898419/range1898419/1
type: Range
persistent identity: range1898419
display id: range1898419
version: 1
direction: direction/forward
top level: BBa_B1003
start: 26
end: 30
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898417
display id: annotation1898417
version: 1
top level: BBa_B1003
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898417/range1898417/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898417/range1898417/1
type: Range
persistent identity: range1898417
display id: range1898417
version: 1
direction: direction/forward
top level: BBa_B1003
start: 10
end: 25
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898418
display id: annotation1898418
version: 1
top level: BBa_B1003
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898418/range1898418/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898418/range1898418/1
type: Range
persistent identity: range1898418
display id: range1898418
version: 1
direction: direction/reverse
top level: BBa_B1003
start: 5
end: 9
orientation: inline

B1003
type: SequenceAnnotation
persistent identity: annotation1898416
display id: annotation1898416
version: 1
top level: BBa_B1003
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1003/annotation1898416/range1898416/1

https://synbiohub.org/public/igem/BBa_B1003/annotation1898416/range1898416/1
type: Range
persistent identity: range1898416
display id: range1898416
version: 1
direction: direction/forward
top level: BBa_B1003
start: 1
end: 34
orientation: inline

BBa_I6116
type: ComponentDefinition
persistent identity: BBa I6116
display id: BBa_I6116
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0077.E0030.B0015
creator: jcbraff
created: 2004-06-08T11:00:00Z
modified: 2015-08-31T04:07:44Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_R0077
component: BBa_B0012
component: BBa_B0010
component: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence annotation: BBa_R0077
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I6116_sequence/1

BBa_R0077
type: Component
persistent identity: component943811
display id: component943811
version: 1
top level: BBa_I6116
access: public
definition: cinR

BBa_B0012
type: Component
persistent identity: component943828
display id: component943828
version: 1
top level: BBa_I6116
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component943818
display id: component943818
version: 1
top level: BBa_I6116
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component943813
display id: component943813
version: 1
top level: BBa_I6116
access: public
definition: eyfp

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943818
display id: annotation943818
version: 1
top level: BBa_I6116
location: https://synbiohub.org/public/igem/BBa_I6116/annotation943818/range943818/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6116/annotation943818/range943818/1
type: Range
persistent identity: range943818
display id: range943818
version: 1
direction: direction/forward
top level: BBa_I6116
start: 969
end: 1048
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943813
display id: annotation943813
version: 1
top level: BBa_I6116
location: https://synbiohub.org/public/igem/BBa_I6116/annotation943813/range943813/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I6116/annotation943813/range943813/1
type: Range
persistent identity: range943813
display id: range943813
version: 1
direction: direction/forward
top level: BBa_I6116
start: 238
end: 960
orientation: inline

BBa_R0077
type: SequenceAnnotation
persistent identity: annotation943811
display id: annotation943811
version: 1
top level: BBa_I6116
location: https://synbiohub.org/public/igem/BBa_I6116/annotation943811/range943811/1
component: BBa_R0077

https://synbiohub.org/public/igem/BBa_I6116/annotation943811/range943811/1
type: Range
persistent identity: range943811
display id: range943811
version: 1
direction: direction/forward
top level: BBa_I6116
start: 1
end: 231
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943828
display id: annotation943828
version: 1
top level: BBa_I6116
location: https://synbiohub.org/public/igem/BBa_I6116/annotation943828/range943828/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6116/annotation943828/range943828/1
type: Range
persistent identity: range943828
display id: range943828
version: 1
direction: direction/forward
top level: BBa_I6116
start: 1057
end: 1097
orientation: inline

BBa_I746909
type: ComponentDefinition
persistent identity: BBa I746909
display id: BBa_I746909
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: superfolder GFP driven by T7 promoter
creator: Stefan Milde
created: 2008-09-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is one of the constructs used to characterise superfolder GFP (see I746916 part description for source and other information about this GFP variant)
mutable notes: no special considerations
mutable provenance: This part was created by standard assembly from construction intermediates: I746911 was put together with I746916:B0015
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_I719005
component: BBa_B0012
component: BBa_I746916
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_I746916
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I719005
sequence: https://synbiohub.org/public/igem/BBa_I746909_sequence/1

BBa_I719005
type: Component
persistent identity: component1977593
display id: component1977593
version: 1
top level: BBa_I746909
access: public
definition: https://synbiohub.org/public/igem/BBa_I719005/1

BBa_B0012
type: Component
persistent identity: component1977602
display id: component1977602
version: 1
top level: BBa_I746909
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I746916
type: Component
persistent identity: component1977599
display id: component1977599
version: 1
top level: BBa_I746909
access: public
definition: https://synbiohub.org/public/igem/BBa_I746916/1

BBa_B0010
type: Component
persistent identity: component1977600
display id: component1977600
version: 1
top level: BBa_I746909
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1977595
display id: component1977595
version: 1
top level: BBa_I746909
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation1977599
display id: annotation1977599
version: 1
top level: BBa_I746909
location: https://synbiohub.org/public/igem/BBa_I746909/annotation1977599/range1977599/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_I746909/annotation1977599/range1977599/1
type: Range
persistent identity: range1977599
display id: range1977599
version: 1
direction: direction/reverse
top level: BBa_I746909
start: 50
end: 769
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1977595
display id: annotation1977595
version: 1
top level: BBa_I746909
location: https://synbiohub.org/public/igem/BBa_I746909/annotation1977595/range1977595/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746909/annotation1977595/range1977595/1
type: Range
persistent identity: range1977595
display id: range1977595
version: 1
direction: direction/reverse
top level: BBa_I746909
start: 32
end: 43
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1977600
display id: annotation1977600
version: 1
top level: BBa_I746909
location: https://synbiohub.org/public/igem/BBa_I746909/annotation1977600/range1977600/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746909/annotation1977600/range1977600/1
type: Range
persistent identity: range1977600
display id: range1977600
version: 1
direction: direction/forward
top level: BBa_I746909
start: 778
end: 857
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1977602
display id: annotation1977602
version: 1
top level: BBa_I746909
location: https://synbiohub.org/public/igem/BBa_I746909/annotation1977602/range1977602/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746909/annotation1977602/range1977602/1
type: Range
persistent identity: range1977602
display id: range1977602
version: 1
direction: direction/forward
top level: BBa_I746909
start: 866
end: 906
orientation: inline

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation1977593
display id: annotation1977593
version: 1
top level: BBa_I746909
location: https://synbiohub.org/public/igem/BBa_I746909/annotation1977593/range1977593/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_I746909/annotation1977593/range1977593/1
type: Range
persistent identity: range1977593
display id: range1977593
version: 1
direction: direction/reverse
top level: BBa_I746909
start: 1
end: 23
orientation: inline

T7rnap
type: ComponentDefinition
persistent identity: BBa I716103
display id: BBa_I716103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7rnap (T7 RNA Polymerase) - ATG Start Codon
creator: David Tulga
created: 2007-06-08T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _110_
m user id: 0
owner id: 1694
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the T7 RNA Polymerase gene's Open Reading Frame, and it will then transcribe any genes with a T7 Promoter, when this polymerase is produced. It provides very good PoPS (Polymerases Per Second) output, as well as it is highly orthogonal to E. Coli's natural transcription system.
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: The source is from pBAC-T7621.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: T7rnap ATG Start
sequence: https://synbiohub.org/public/igem/BBa_I716103_sequence/1

T7rnap ATG Start
type: SequenceAnnotation
persistent identity: annotation1954615
display id: annotation1954615
version: 1
top level: T7rnap
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716103/annotation1954615/range1954615/1

https://synbiohub.org/public/igem/BBa_I716103/annotation1954615/range1954615/1
type: Range
persistent identity: range1954615
display id: range1954615
version: 1
direction: direction/forward
top level: T7rnap
start: 1
end: 2652
orientation: inline

BBa_K118007
type: ComponentDefinition
persistent identity: BBa K118007
display id: BBa_K118007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+appY (encoding a DNA-binding transcriptional activator)
creator: Andrew Hall
created: 2008-08-07T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of appY from Escherichia coli JM109 preceded by a synthetic ribosome binding site (rbs). It encodes a transcriptional regulator related to anaerobic energy metabolism. Overexpression of the gene has been reported to result in enhanced lycopene production by E. coli (Kang et al., 2005).
mutable notes: No special considerations
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_K118001
sequence annotation: BBa_K118001
sequence annotation: BBa_J15001
sequence: https://synbiohub.org/public/igem/BBa_K118007_sequence/1

BBa_J15001
type: Component
persistent identity: component1970367
display id: component1970367
version: 1
top level: BBa_K118007
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118001
type: Component
persistent identity: component1970368
display id: component1970368
version: 1
top level: BBa_K118007
access: public
definition: https://synbiohub.org/public/igem/BBa_K118001/1

BBa_K118001
type: SequenceAnnotation
persistent identity: annotation1970368
display id: annotation1970368
version: 1
top level: BBa_K118007
location: https://synbiohub.org/public/igem/BBa_K118007/annotation1970368/range1970368/1
component: BBa_K118001

https://synbiohub.org/public/igem/BBa_K118007/annotation1970368/range1970368/1
type: Range
persistent identity: range1970368
display id: range1970368
version: 1
direction: direction/reverse
top level: BBa_K118007
start: 17
end: 769
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1970367
display id: annotation1970367
version: 1
top level: BBa_K118007
location: https://synbiohub.org/public/igem/BBa_K118007/annotation1970367/range1970367/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118007/annotation1970367/range1970367/1
type: Range
persistent identity: range1970367
display id: range1970367
version: 1
direction: direction/reverse
top level: BBa_K118007
start: 1
end: 10
orientation: inline

BBa_I746370
type: ComponentDefinition
persistent identity: BBa I746370
display id: BBa_I746370
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P2 ogr inducing PF promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the P2 ogr activator on the PF promoter.
mutable notes: I746313 was put upstream of I746320
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746313
component: BBa_I746320
sequence annotation: BBa_I746313
sequence annotation: BBa_I746320
sequence: https://synbiohub.org/public/igem/BBa_I746370_sequence/1

BBa_I746313
type: Component
persistent identity: component2291673
display id: component2291673
version: 1
top level: BBa_I746370
access: public
definition: https://synbiohub.org/public/igem/BBa_I746313/1

BBa_I746320
type: Component
persistent identity: component2291686
display id: component2291686
version: 1
top level: BBa_I746370
access: public
definition: BBa_I746320

BBa_I746313
type: SequenceAnnotation
persistent identity: annotation2291673
display id: annotation2291673
version: 1
top level: BBa_I746370
location: https://synbiohub.org/public/igem/BBa_I746370/annotation2291673/range2291673/1
component: BBa_I746313

https://synbiohub.org/public/igem/BBa_I746370/annotation2291673/range2291673/1
type: Range
persistent identity: range2291673
display id: range2291673
version: 1
direction: direction/reverse
top level: BBa_I746370
start: 1
end: 2324
orientation: inline

BBa_I746320
type: SequenceAnnotation
persistent identity: annotation2291686
display id: annotation2291686
version: 1
top level: BBa_I746370
location: https://synbiohub.org/public/igem/BBa_I746370/annotation2291686/range2291686/1
component: BBa_I746320

https://synbiohub.org/public/igem/BBa_I746370/annotation2291686/range2291686/1
type: Range
persistent identity: range2291686
display id: range2291686
version: 1
direction: direction/reverse
top level: BBa_I746370
start: 2333
end: 3306
orientation: inline

BBa_J52008
type: ComponentDefinition
persistent identity: BBa J52008
display id: BBa_J52008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
creator: Monika Ciglic
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Rluc is a protein called Renilla`s luciferase and it emits light when adding the right substrate. That is why it can bi used as a reported to track other proteins or to monitor the activity of a promotor.
mutable notes: Rluc is cloned in BioBrick vector pSB1AK3.
mutable provenance: -
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52008_sequence/1

Rluc
type: SequenceAnnotation
persistent identity: annotation1902833
display id: annotation1902833
version: 1
top level: BBa_J52008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1

https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
type: Range
persistent identity: range1902833
display id: range1902833
version: 1
direction: direction/forward
top level: BBa_J52008
start: 1
end: 936
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902845
display id: annotation1902845
version: 1
top level: BBa_J52008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1

https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
type: Range
persistent identity: range1902845
display id: range1902845
version: 1
direction: direction/forward
top level: BBa_J52008
start: 1
end: 936
orientation: inline

BBa_I13522
type: ComponentDefinition
persistent identity: BBa I13522
display id: BBa_I13522
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet GFP
creator: jkm
created: 2005-06-30T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Untagged GFP behind a constitutive promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0034
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13522_sequence/1

BBa_B0034
type: Component
persistent identity: component1559464
display id: component1559464
version: 1
top level: BBa_I13522
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1559466
display id: component1559466
version: 1
top level: BBa_I13522
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1559481
display id: component1559481
version: 1
top level: BBa_I13522
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1559471
display id: component1559471
version: 1
top level: BBa_I13522
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_R0040
type: Component
persistent identity: component1559456
display id: component1559456
version: 1
top level: BBa_I13522
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1559456
display id: annotation1559456
version: 1
top level: BBa_I13522
location: https://synbiohub.org/public/igem/BBa_I13522/annotation1559456/range1559456/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13522/annotation1559456/range1559456/1
type: Range
persistent identity: range1559456
display id: range1559456
version: 1
direction: direction/forward
top level: BBa_I13522
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1559481
display id: annotation1559481
version: 1
top level: BBa_I13522
location: https://synbiohub.org/public/igem/BBa_I13522/annotation1559481/range1559481/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13522/annotation1559481/range1559481/1
type: Range
persistent identity: range1559481
display id: range1559481
version: 1
direction: direction/forward
top level: BBa_I13522
start: 897
end: 937
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1559471
display id: annotation1559471
version: 1
top level: BBa_I13522
location: https://synbiohub.org/public/igem/BBa_I13522/annotation1559471/range1559471/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13522/annotation1559471/range1559471/1
type: Range
persistent identity: range1559471
display id: range1559471
version: 1
direction: direction/forward
top level: BBa_I13522
start: 809
end: 888
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1559464
display id: annotation1559464
version: 1
top level: BBa_I13522
location: https://synbiohub.org/public/igem/BBa_I13522/annotation1559464/range1559464/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13522/annotation1559464/range1559464/1
type: Range
persistent identity: range1559464
display id: range1559464
version: 1
direction: direction/forward
top level: BBa_I13522
start: 63
end: 74
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1559466
display id: annotation1559466
version: 1
top level: BBa_I13522
location: https://synbiohub.org/public/igem/BBa_I13522/annotation1559466/range1559466/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13522/annotation1559466/range1559466/1
type: Range
persistent identity: range1559466
display id: range1559466
version: 1
direction: direction/forward
top level: BBa_I13522
start: 81
end: 800
orientation: inline

BBa_I13500
type: ComponentDefinition
persistent identity: BBa I13500
display id: BBa_I13500
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I13500_sequence/1

BBa_B0034
type: Component
persistent identity: component1505000
display id: component1505000
version: 1
top level: BBa_I13500
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1505002
display id: component1505002
version: 1
top level: BBa_I13500
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505000
display id: annotation1505000
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13500/annotation1505000/range1505000/1
type: Range
persistent identity: range1505000
display id: range1505000
version: 1
direction: direction/forward
top level: BBa_I13500
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505002
display id: annotation1505002
version: 1
top level: BBa_I13500
location: https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13500/annotation1505002/range1505002/1
type: Range
persistent identity: range1505002
display id: range1505002
version: 1
direction: direction/forward
top level: BBa_I13500
start: 19
end: 738
orientation: inline

ATF1
type: ComponentDefinition
persistent identity: BBa J45014
display id: BBa_J45014
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)
creator: Andr?? Green II
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T04:08:49Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part is the ATF sequence (BBa_45J006) with a single point mutation at base pair 1176 (T -> C).
mutable notes: It has a single point mutation at base pair 1176 (T -> C).
mutable provenance: This part comes from BBa_45J006.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATF1
sequence annotation: t -> c
sequence annotation: double TAA stop codon
sequence annotation: a->g to eliminate EcoRI site
sequence: https://synbiohub.org/public/igem/BBa_J45014_sequence/1

ATF1
type: SequenceAnnotation
persistent identity: annotation1961300
display id: annotation1961300
version: 1
top level: ATF1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1
type: Range
persistent identity: range1961300
display id: range1961300
version: 1
direction: direction/forward
top level: ATF1
start: 1
end: 1581
orientation: inline

t -> c
type: SequenceAnnotation
persistent identity: annotation1961301
display id: annotation1961301
version: 1
top level: ATF1
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1
type: Range
persistent identity: range1961301
display id: range1961301
version: 1
direction: direction/forward
top level: ATF1
start: 1148
end: 1148
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1961302
display id: annotation1961302
version: 1
top level: ATF1
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1
type: Range
persistent identity: range1961302
display id: range1961302
version: 1
direction: direction/forward
top level: ATF1
start: 1576
end: 1581
orientation: inline

a->g to eliminate EcoRI site
type: SequenceAnnotation
persistent identity: annotation1961305
display id: annotation1961305
version: 1
top level: ATF1
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1

https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1
type: Range
persistent identity: range1961305
display id: range1961305
version: 1
direction: direction/forward
top level: ATF1
start: 414
end: 414
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa J04650
display id: BBa_J04650
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E1010.B0015
creator: Kristen DeCelle
created: 2005-08-28T11:00:00Z
modified: 2015-08-31T04:08:14Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part results from ligating E1010 with B0015 in the manner described on this website.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_B0012
component: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_J04650_sequence/1

BBa_B0010
type: Component
persistent identity: component1676942
display id: component1676942
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1676952
display id: component1676952
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E1010
type: Component
persistent identity: component1676935
display id: component1676935
version: 1
top level: mRFP1
access: public
definition: mRFP1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1676952
display id: annotation1676952
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_J04650/annotation1676952/range1676952/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J04650/annotation1676952/range1676952/1
type: Range
persistent identity: range1676952
display id: range1676952
version: 1
direction: direction/forward
top level: mRFP1
start: 803
end: 843
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1676935
display id: annotation1676935
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_J04650/annotation1676935/range1676935/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_J04650/annotation1676935/range1676935/1
type: Range
persistent identity: range1676935
display id: range1676935
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 681
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1676942
display id: annotation1676942
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_J04650/annotation1676942/range1676942/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J04650/annotation1676942/range1676942/1
type: Range
persistent identity: range1676942
display id: range1676942
version: 1
direction: direction/forward
top level: mRFP1
start: 715
end: 794
orientation: inline

BBa_I13544
type: ComponentDefinition
persistent identity: BBa I13544
display id: BBa_I13544
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Intermediate in two promoter screening plasmid
creator: Josh Michener
created: 2005-08-21T11:00:00Z
modified: 2015-08-31T04:07:35Z
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13458
component: BBa_E1010
component: BBa_B0012
component: BBa_I13457
component: BBa_B0034
component: BBa_I13453
component: BBa_B0010
sequence annotation: BBa_I13458
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0012
sequence annotation: BBa_I13453
sequence annotation: BBa_B0010
sequence annotation: BBa_I13457
sequence: https://synbiohub.org/public/igem/BBa_I13544_sequence/1

BBa_I13458
type: Component
persistent identity: component1686755
display id: component1686755
version: 1
top level: BBa_I13544
access: public
definition: BBa_I13458

BBa_E1010
type: Component
persistent identity: component1686789
display id: component1686789
version: 1
top level: BBa_I13544
access: public
definition: mRFP1

BBa_B0012
type: Component
persistent identity: component1686805
display id: component1686805
version: 1
top level: BBa_I13544
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I13457
type: Component
persistent identity: component1686777
display id: component1686777
version: 1
top level: BBa_I13544
access: public
definition: https://synbiohub.org/public/igem/BBa_I13457/1

BBa_B0034
type: Component
persistent identity: component1686782
display id: component1686782
version: 1
top level: BBa_I13544
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13453
type: Component
persistent identity: component1686757
display id: component1686757
version: 1
top level: BBa_I13544
access: public
definition: BBa_I13453

BBa_B0010
type: Component
persistent identity: component1686795
display id: component1686795
version: 1
top level: BBa_I13544
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation1686755
display id: annotation1686755
version: 1
top level: BBa_I13544
location: https://synbiohub.org/public/igem/BBa_I13544/annotation1686755/range1686755/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_I13544/annotation1686755/range1686755/1
type: Range
persistent identity: range1686755
display id: range1686755
version: 1
direction: direction/forward
top level: BBa_I13544
start: 1
end: 1114
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1686782
display id: annotation1686782
version: 1
top level: BBa_I13544
location: https://synbiohub.org/public/igem/BBa_I13544/annotation1686782/range1686782/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13544/annotation1686782/range1686782/1
type: Range
persistent identity: range1686782
display id: range1686782
version: 1
direction: direction/forward
top level: BBa_I13544
start: 1380
end: 1391
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1686789
display id: annotation1686789
version: 1
top level: BBa_I13544
location: https://synbiohub.org/public/igem/BBa_I13544/annotation1686789/range1686789/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13544/annotation1686789/range1686789/1
type: Range
persistent identity: range1686789
display id: range1686789
version: 1
direction: direction/forward
top level: BBa_I13544
start: 1398
end: 2078
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1686805
display id: annotation1686805
version: 1
top level: BBa_I13544
location: https://synbiohub.org/public/igem/BBa_I13544/annotation1686805/range1686805/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13544/annotation1686805/range1686805/1
type: Range
persistent identity: range1686805
display id: range1686805
version: 1
direction: direction/forward
top level: BBa_I13544
start: 2200
end: 2240
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1686757
display id: annotation1686757
version: 1
top level: BBa_I13544
location: https://synbiohub.org/public/igem/BBa_I13544/annotation1686757/range1686757/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_I13544/annotation1686757/range1686757/1
type: Range
persistent identity: range1686757
display id: range1686757
version: 1
direction: direction/forward
top level: BBa_I13544
start: 1123
end: 1252
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1686795
display id: annotation1686795
version: 1
top level: BBa_I13544
location: https://synbiohub.org/public/igem/BBa_I13544/annotation1686795/range1686795/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13544/annotation1686795/range1686795/1
type: Range
persistent identity: range1686795
display id: range1686795
version: 1
direction: direction/forward
top level: BBa_I13544
start: 2112
end: 2191
orientation: inline

BBa_I13457
type: SequenceAnnotation
persistent identity: annotation1686777
display id: annotation1686777
version: 1
top level: BBa_I13544
location: https://synbiohub.org/public/igem/BBa_I13544/annotation1686777/range1686777/1
component: BBa_I13457

https://synbiohub.org/public/igem/BBa_I13544/annotation1686777/range1686777/1
type: Range
persistent identity: range1686777
display id: range1686777
version: 1
direction: direction/forward
top level: BBa_I13544
start: 1261
end: 1371
orientation: inline

BBa_K117000
type: ComponentDefinition
persistent identity: BBa K117000
display id: BBa_K117000
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Lysis gene (promotes lysis in colicin-producing bacteria strain)
creator: Nguyen Xuan Hung
created: 2008-10-06T11:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _209_
m user id: 0
owner id: 2774
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This lysis gene encodes for the lysis protein in colicin-producing strains of bacteria. Once activated, it causes the host cell to lyze. It also removes the immunity protein out of colicin, and hence, activates the endonuclease activity of the colicin.
mutable notes: to be updated
mutable provenance: to be updated
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K117000_sequence/1

BBa_I746364
type: ComponentDefinition
persistent identity: BBa I746364
display id: BBa_I746364
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Psid promoter from P4 phage
creator: Stefan Milde
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the Psid promoter taken from the P4 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the Psid promoter.
mutable notes: no special considerations
mutable provenance: P4 phage genome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Psid
sequence: https://synbiohub.org/public/igem/BBa_I746364_sequence/1

Psid
type: SequenceAnnotation
persistent identity: annotation1943787
display id: annotation1943787
version: 1
top level: BBa_I746364
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I746364/annotation1943787/range1943787/1

https://synbiohub.org/public/igem/BBa_I746364/annotation1943787/range1943787/1
type: Range
persistent identity: range1943787
display id: range1943787
version: 1
direction: direction/forward
top level: BBa_I746364
start: 1
end: 93
orientation: inline

BBa_J23040
type: ComponentDefinition
persistent identity: BBa J23040
display id: BBa_J23040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [TetR][rbs][LuxR][dblTerm][LuxPR]+[rbs][LacI][dblTerm] "AHL-dependent inverter"
creator: Will Bosworth
created: 2006-07-31T11:00:00Z
modified: 2015-08-31T04:08:39Z
discontinued: false
dominant: false
group u list: _52_
m user id: 0
owner id: 363
owning group id: 52
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: F2620+P0412
mutable notes: part of a NAND-gate system with parts ____
mutable provenance: parts source: iGEM headquarters
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_F2620
component: BBa_P0412
sequence annotation: BBa_P0412
sequence annotation: BBa_F2620
sequence: https://synbiohub.org/public/igem/BBa_J23040_sequence/1

BBa_F2620
type: Component
persistent identity: component2278751
display id: component2278751
version: 1
top level: BBa_J23040
access: public
definition: BBa_F2620

BBa_P0412
type: Component
persistent identity: component2278764
display id: component2278764
version: 1
top level: BBa_J23040
access: public
definition: BBa_P0412

BBa_P0412
type: SequenceAnnotation
persistent identity: annotation2278764
display id: annotation2278764
version: 1
top level: BBa_J23040
location: https://synbiohub.org/public/igem/BBa_J23040/annotation2278764/range2278764/1
component: BBa_P0412

https://synbiohub.org/public/igem/BBa_J23040/annotation2278764/range2278764/1
type: Range
persistent identity: range2278764
display id: range2278764
version: 1
direction: direction/reverse
top level: BBa_J23040
start: 1070
end: 2377
orientation: inline

BBa_F2620
type: SequenceAnnotation
persistent identity: annotation2278751
display id: annotation2278751
version: 1
top level: BBa_J23040
location: https://synbiohub.org/public/igem/BBa_J23040/annotation2278751/range2278751/1
component: BBa_F2620

https://synbiohub.org/public/igem/BBa_J23040/annotation2278751/range2278751/1
type: Range
persistent identity: range2278751
display id: range2278751
version: 1
direction: direction/reverse
top level: BBa_J23040
start: 1
end: 1061
orientation: inline

BBa_I13605
type: ComponentDefinition
persistent identity: BBa I13605
display id: BBa_I13605
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reporter Construct (Ly-Tc-) for constitutive CFP and inducible YFP
creator: Chris Batton, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reporter plasmid with Lac operator+YFP(LVA-) reporter followed by a Tet operator+CFP(LVA-) reporter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_R0040
component: BBa_E0420
component: BBa_I6031
sequence annotation: BBa_I6031
sequence annotation: BBa_E0420
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_I13605_sequence/1

BBa_R0040
type: Component
persistent identity: component2228641
display id: component2228641
version: 1
top level: BBa_I13605
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0420
type: Component
persistent identity: component2228655
display id: component2228655
version: 1
top level: BBa_I13605
access: public
definition: BBa_E0420

BBa_I6031
type: Component
persistent identity: component2228640
display id: component2228640
version: 1
top level: BBa_I13605
access: public
definition: https://synbiohub.org/public/igem/BBa_I6031/1

BBa_I6031
type: SequenceAnnotation
persistent identity: annotation2228640
display id: annotation2228640
version: 1
top level: BBa_I13605
location: https://synbiohub.org/public/igem/BBa_I13605/annotation2228640/range2228640/1
component: BBa_I6031

https://synbiohub.org/public/igem/BBa_I13605/annotation2228640/range2228640/1
type: Range
persistent identity: range2228640
display id: range2228640
version: 1
direction: direction/reverse
top level: BBa_I13605
start: 1
end: 941
orientation: inline

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2228655
display id: annotation2228655
version: 1
top level: BBa_I13605
location: https://synbiohub.org/public/igem/BBa_I13605/annotation2228655/range2228655/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_I13605/annotation2228655/range2228655/1
type: Range
persistent identity: range2228655
display id: range2228655
version: 1
direction: direction/reverse
top level: BBa_I13605
start: 1012
end: 1889
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2228641
display id: annotation2228641
version: 1
top level: BBa_I13605
location: https://synbiohub.org/public/igem/BBa_I13605/annotation2228641/range2228641/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13605/annotation2228641/range2228641/1
type: Range
persistent identity: range2228641
display id: range2228641
version: 1
direction: direction/forward
top level: BBa_I13605
start: 950
end: 1003
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS.1 (strong) -- modified from R. Weiss
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
role: feature/rbs
role: SO:0000139
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
direction: direction/forward
top level: BBa_B0030
start: 8
end: 12
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
direction: direction/forward
top level: BBa_B0030
start: 1
end: 15
orientation: inline

BBa_I716208
type: ComponentDefinition
persistent identity: BBa I716208
display id: BBa_I716208
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [lox][terminator][lox]
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: [lox][terminator][lox]
mutable notes: Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large) Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) Product is pBca9145-I716208.
mutable provenance: Digest pBca9145-I716206 (BamHI/AlwnI, 1694+553bp, Large) Digest pBca9145-Bca1114 (BglII/AlwnI, 1519+593bp, Small) Product is pBca9145-I716208.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence: https://synbiohub.org/public/igem/BBa_I716208_sequence/1

BBa_S03119
type: ComponentDefinition
persistent identity: BBa S03119
display id: BBa_S03119
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: --Specify Parts List--
creator: Randy Rettberg
created: 2004-04-19T11:00:00Z
modified: 2015-05-08T01:14:19Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate part from assembly 299
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_R0040
component: BBa_C0062
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_C0062
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_S03119_sequence/1

BBa_B0034
type: Component
persistent identity: component955034
display id: component955034
version: 1
top level: BBa_S03119
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_R0040
type: Component
persistent identity: component955026
display id: component955026
version: 1
top level: BBa_S03119
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0062
type: Component
persistent identity: component955049
display id: component955049
version: 1
top level: BBa_S03119
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_B0012
type: Component
persistent identity: component955067
display id: component955067
version: 1
top level: BBa_S03119
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component955057
display id: component955057
version: 1
top level: BBa_S03119
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation955067
display id: annotation955067
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955067/range955067/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03119/annotation955067/range955067/1
type: Range
persistent identity: range955067
display id: range955067
version: 1
direction: direction/forward
top level: BBa_S03119
start: 958
end: 998
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation955026
display id: annotation955026
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955026/range955026/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_S03119/annotation955026/range955026/1
type: Range
persistent identity: range955026
display id: range955026
version: 1
direction: direction/forward
top level: BBa_S03119
start: 1
end: 54
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation955049
display id: annotation955049
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955049/range955049/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_S03119/annotation955049/range955049/1
type: Range
persistent identity: range955049
display id: range955049
version: 1
direction: direction/forward
top level: BBa_S03119
start: 81
end: 836
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation955034
display id: annotation955034
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955034/range955034/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03119/annotation955034/range955034/1
type: Range
persistent identity: range955034
display id: range955034
version: 1
direction: direction/forward
top level: BBa_S03119
start: 63
end: 74
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation955057
display id: annotation955057
version: 1
top level: BBa_S03119
location: https://synbiohub.org/public/igem/BBa_S03119/annotation955057/range955057/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03119/annotation955057/range955057/1
type: Range
persistent identity: range955057
display id: range955057
version: 1
direction: direction/forward
top level: BBa_S03119
start: 870
end: 949
orientation: inline

BBa_I746385
type: ComponentDefinition
persistent identity: BBa I746385
display id: BBa_I746385
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PSP3 pag inducing PLL promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the PSP3 pag activator on the PLL promoter. Note that the I746351 does contain a RBS.
mutable notes: I746314 was put upstream of I746325
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746314
component: BBa_I746325
sequence annotation: BBa_I746325
sequence annotation: BBa_I746314
sequence: https://synbiohub.org/public/igem/BBa_I746385_sequence/1

BBa_I746314
type: Component
persistent identity: component2377058
display id: component2377058
version: 1
top level: BBa_I746385
access: public
definition: https://synbiohub.org/public/igem/BBa_I746314/1

BBa_I746325
type: Component
persistent identity: component2377071
display id: component2377071
version: 1
top level: BBa_I746385
access: public
definition: https://synbiohub.org/public/igem/BBa_I746325/1

BBa_I746325
type: SequenceAnnotation
persistent identity: annotation2377071
display id: annotation2377071
version: 1
top level: BBa_I746385
location: https://synbiohub.org/public/igem/BBa_I746385/annotation2377071/range2377071/1
component: BBa_I746325

https://synbiohub.org/public/igem/BBa_I746385/annotation2377071/range2377071/1
type: Range
persistent identity: range2377071
display id: range2377071
version: 1
direction: direction/reverse
top level: BBa_I746385
start: 2333
end: 3307
orientation: inline

BBa_I746314
type: SequenceAnnotation
persistent identity: annotation2377058
display id: annotation2377058
version: 1
top level: BBa_I746385
location: https://synbiohub.org/public/igem/BBa_I746385/annotation2377058/range2377058/1
component: BBa_I746314

https://synbiohub.org/public/igem/BBa_I746385/annotation2377058/range2377058/1
type: Range
persistent identity: range2377058
display id: range2377058
version: 1
direction: direction/reverse
top level: BBa_I746385
start: 1
end: 2324
orientation: inline

mnt (w)
type: ComponentDefinition
persistent identity: BBa C0073
display id: BBa_C0073
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: mnt repressor (weak) from Salmonella phage P22 (+LVA)
creator: crackdots
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
mutable notes: dimeric repressor from enterobacteriophage p22 with dissociation constant of 3*10^-8. sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99). the mutation R10->K10 (ctg->aaa) decreases the binding affinity of the mnt protein to its operator by approximately 12,000 fold (Kd: 2.5e-12 to 3e-8)
mutable provenance: enterobacteriophage p22
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T
sequence annotation: mnt
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence annotation: Arg->Lys (ctg)
sequence: https://synbiohub.org/public/igem/BBa_C0073_sequence/1

T
type: SequenceAnnotation
persistent identity: annotation308457
display id: annotation308457
version: 1
top level: mnt (w)
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_C0073/annotation308457/range308457/1

https://synbiohub.org/public/igem/BBa_C0073/annotation308457/range308457/1
type: Range
persistent identity: range308457
display id: range308457
version: 1
direction: direction/forward
top level: mnt (w)
start: 95
end: 97
orientation: inline

mnt
type: SequenceAnnotation
persistent identity: annotation302631
display id: annotation302631
version: 1
top level: mnt (w)
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0073/annotation302631/range302631/1

https://synbiohub.org/public/igem/BBa_C0073/annotation302631/range302631/1
type: Range
persistent identity: range302631
display id: range302631
version: 1
direction: direction/forward
top level: mnt (w)
start: 1
end: 249
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306535
display id: annotation306535
version: 1
top level: mnt (w)
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0073/annotation306535/range306535/1

https://synbiohub.org/public/igem/BBa_C0073/annotation306535/range306535/1
type: Range
persistent identity: range306535
display id: range306535
version: 1
direction: direction/forward
top level: mnt (w)
start: 250
end: 282
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214006
display id: annotation2214006
version: 1
top level: mnt (w)
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0073/annotation2214006/range2214006/1

https://synbiohub.org/public/igem/BBa_C0073/annotation2214006/range2214006/1
type: Range
persistent identity: range2214006
display id: range2214006
version: 1
direction: direction/forward
top level: mnt (w)
start: 289
end: 313
orientation: inline

Arg->Lys (ctg)
type: SequenceAnnotation
persistent identity: annotation308213
display id: annotation308213
version: 1
top level: mnt (w)
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_C0073/annotation308213/range308213/1

https://synbiohub.org/public/igem/BBa_C0073/annotation308213/range308213/1
type: Range
persistent identity: range308213
display id: range308213
version: 1
direction: direction/forward
top level: mnt (w)
start: 31
end: 33
orientation: inline

BBa_I746371
type: ComponentDefinition
persistent identity: BBa I746371
display id: BBa_I746371
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P2 ogr inducing PO promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the P2 ogr activator on the PO promoter.
mutable notes: I746313 was put upstream of I746321
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746321
component: BBa_I746313
sequence annotation: BBa_I746313
sequence annotation: BBa_I746321
sequence: https://synbiohub.org/public/igem/BBa_I746371_sequence/1

BBa_I746321
type: Component
persistent identity: component2291721
display id: component2291721
version: 1
top level: BBa_I746371
access: public
definition: https://synbiohub.org/public/igem/BBa_I746321/1

BBa_I746313
type: Component
persistent identity: component2291708
display id: component2291708
version: 1
top level: BBa_I746371
access: public
definition: https://synbiohub.org/public/igem/BBa_I746313/1

BBa_I746313
type: SequenceAnnotation
persistent identity: annotation2291708
display id: annotation2291708
version: 1
top level: BBa_I746371
location: https://synbiohub.org/public/igem/BBa_I746371/annotation2291708/range2291708/1
component: BBa_I746313

https://synbiohub.org/public/igem/BBa_I746371/annotation2291708/range2291708/1
type: Range
persistent identity: range2291708
display id: range2291708
version: 1
direction: direction/reverse
top level: BBa_I746371
start: 1
end: 2324
orientation: inline

BBa_I746321
type: SequenceAnnotation
persistent identity: annotation2291721
display id: annotation2291721
version: 1
top level: BBa_I746371
location: https://synbiohub.org/public/igem/BBa_I746371/annotation2291721/range2291721/1
component: BBa_I746321

https://synbiohub.org/public/igem/BBa_I746371/annotation2291721/range2291721/1
type: Range
persistent identity: range2291721
display id: range2291721
version: 1
direction: direction/reverse
top level: BBa_I746371
start: 2333
end: 3307
orientation: inline

BBa_K203118
type: ComponentDefinition
persistent identity: BBa K203118
display id: BBa_K203118
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Constitutive promoter; 1 REU
creator: Lars Velten, Simon Haas, Hannah Meyer, Anne Rademacher, Hannah Uckelmann and Corinna Hiller
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:22Z
discontinued: false
dominant: false
experience: experience/None
group u list: _301_
m user id: 0
owner id: 5074
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: To be updated
mutable notes: To be updated
mutable provenance: To be updated
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: NFkB Binding Site
sequence annotation: JeT Core promoter
sequence annotation: NfkB Binding site
sequence annotation: CREB Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K203118_sequence/1

NFkB Binding Site
type: SequenceAnnotation
persistent identity: annotation2046078
display id: annotation2046078
version: 1
top level: BBa_K203118
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203118/annotation2046078/range2046078/1

https://synbiohub.org/public/igem/BBa_K203118/annotation2046078/range2046078/1
type: Range
persistent identity: range2046078
display id: range2046078
version: 1
direction: direction/forward
top level: BBa_K203118
start: 94
end: 105
orientation: inline

JeT Core promoter
type: SequenceAnnotation
persistent identity: annotation2046094
display id: annotation2046094
version: 1
top level: BBa_K203118
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203118/annotation2046094/range2046094/1

https://synbiohub.org/public/igem/BBa_K203118/annotation2046094/range2046094/1
type: Range
persistent identity: range2046094
display id: range2046094
version: 1
direction: direction/forward
top level: BBa_K203118
start: 229
end: 330
orientation: inline

NfkB Binding site
type: SequenceAnnotation
persistent identity: annotation2046077
display id: annotation2046077
version: 1
top level: BBa_K203118
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203118/annotation2046077/range2046077/1

https://synbiohub.org/public/igem/BBa_K203118/annotation2046077/range2046077/1
type: Range
persistent identity: range2046077
display id: range2046077
version: 1
direction: direction/forward
top level: BBa_K203118
start: 42
end: 53
orientation: inline

CREB Binding Site
type: SequenceAnnotation
persistent identity: annotation2046079
display id: annotation2046079
version: 1
top level: BBa_K203118
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203118/annotation2046079/range2046079/1

https://synbiohub.org/public/igem/BBa_K203118/annotation2046079/range2046079/1
type: Range
persistent identity: range2046079
display id: range2046079
version: 1
direction: direction/forward
top level: BBa_K203118
start: 146
end: 158
orientation: inline

CREB Binding Site
type: SequenceAnnotation
persistent identity: annotation2046092
display id: annotation2046092
version: 1
top level: BBa_K203118
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K203118/annotation2046092/range2046092/1

https://synbiohub.org/public/igem/BBa_K203118/annotation2046092/range2046092/1
type: Range
persistent identity: range2046092
display id: range2046092
version: 1
direction: direction/forward
top level: BBa_K203118
start: 198
end: 210
orientation: inline

BBa_I716210
type: ComponentDefinition
persistent identity: BBa I716210
display id: BBa_I716210
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: BamHI endonuclease without start codon
creator: Samantha Liang
created: 2007-06-04T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: BamHI endonuclease without start codon
mutable notes: PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L) Product is pBca9145-I716210 sil04F Forward BglII biobrick of BamHI ccaaaAGATCTgaagtagaaaaagagtttattactga sil04R Reverse XholI/BamHI biobrick of BamHI gctagCTCGAGttaGGATCCctatttgttttcaactttatc
mutable provenance: PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L) Product is pBca9145-I716210
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716210_sequence/1

PT7-D
type: ComponentDefinition
persistent identity: BBa I716106
display id: BBa_I716106
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: T7 Promoter (Mutant D)
creator: David Tulga
created: 2007-06-08T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _110_
m user id: 0
owner id: 1694
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is the T7 Promoter which is activated when the T7 RNA Polymerase is produced. It provides very good PoPS (Polymerases Per Second) output, as well as it is highly orthogonal to E. Coli's natural transcription system. This one of the moderately active (Mutant D) variations we are using.
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: This part was generated synthetically.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/T7
role: SO:0001207
sequence annotation: T7 Promoter D
sequence: https://synbiohub.org/public/igem/BBa_I716106_sequence/1

T7 Promoter D
type: SequenceAnnotation
persistent identity: annotation1954612
display id: annotation1954612
version: 1
top level: PT7-D
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I716106/annotation1954612/range1954612/1

https://synbiohub.org/public/igem/BBa_I716106/annotation1954612/range1954612/1
type: Range
persistent identity: range1954612
display id: range1954612
version: 1
direction: direction/forward
top level: PT7-D
start: 1
end: 29
orientation: inline

BBa_T9002
type: ComponentDefinition
persistent identity: BBa T9002
display id: BBa_T9002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: GFP Producer Controlled by 3OC6HSL Receiver Device
creator: Anna Labno
created: 2005-06-20T11:00:00Z
modified: 2015-05-08T01:14:52Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _101_
m user id: 0
owner id: 371
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The luxR based receiver, F2620 (formerly I13270), controls the production of GFP. The GFP protein generator is the same as that found in I7101.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_R0040
component: BBa_C0062
component: BBa_R0062
component: BBa_B0032
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0032
sequence annotation: BBa_R0040
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_T9002_sequence/1

BBa_R0040
type: Component
persistent identity: component1565165
display id: component1565165
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_C0062
type: Component
persistent identity: component1565188
display id: component1565188
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_C0062/1

BBa_R0062
type: Component
persistent identity: component1565221
display id: component1565221
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_B0032
type: Component
persistent identity: component1565232
display id: component1565232
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0010
type: Component
persistent identity: component1565196
display id: component1565196
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0040
type: Component
persistent identity: component1565235
display id: component1565235
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0012
type: Component
persistent identity: component1565206
display id: component1565206
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1565173
display id: component1565173
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1565250
display id: component1565250
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1565240
display id: component1565240
version: 1
top level: BBa_T9002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1565232
display id: annotation1565232
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565232/range1565232/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_T9002/annotation1565232/range1565232/1
type: Range
persistent identity: range1565232
display id: range1565232
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1070
end: 1082
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1565165
display id: annotation1565165
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565165/range1565165/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_T9002/annotation1565165/range1565165/1
type: Range
persistent identity: range1565165
display id: range1565165
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1
end: 54
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1565250
display id: annotation1565250
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565250/range1565250/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_T9002/annotation1565250/range1565250/1
type: Range
persistent identity: range1565250
display id: range1565250
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1905
end: 1945
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1565221
display id: annotation1565221
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565221/range1565221/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_T9002/annotation1565221/range1565221/1
type: Range
persistent identity: range1565221
display id: range1565221
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1007
end: 1061
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1565206
display id: annotation1565206
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565206/range1565206/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_T9002/annotation1565206/range1565206/1
type: Range
persistent identity: range1565206
display id: range1565206
version: 1
direction: direction/forward
top level: BBa_T9002
start: 958
end: 998
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1565196
display id: annotation1565196
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565196/range1565196/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_T9002/annotation1565196/range1565196/1
type: Range
persistent identity: range1565196
display id: range1565196
version: 1
direction: direction/forward
top level: BBa_T9002
start: 870
end: 949
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1565235
display id: annotation1565235
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565235/range1565235/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_T9002/annotation1565235/range1565235/1
type: Range
persistent identity: range1565235
display id: range1565235
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1089
end: 1808
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1565240
display id: annotation1565240
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565240/range1565240/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_T9002/annotation1565240/range1565240/1
type: Range
persistent identity: range1565240
display id: range1565240
version: 1
direction: direction/forward
top level: BBa_T9002
start: 1817
end: 1896
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1565173
display id: annotation1565173
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565173/range1565173/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_T9002/annotation1565173/range1565173/1
type: Range
persistent identity: range1565173
display id: range1565173
version: 1
direction: direction/forward
top level: BBa_T9002
start: 63
end: 74
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1565188
display id: annotation1565188
version: 1
top level: BBa_T9002
location: https://synbiohub.org/public/igem/BBa_T9002/annotation1565188/range1565188/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_T9002/annotation1565188/range1565188/1
type: Range
persistent identity: range1565188
display id: range1565188
version: 1
direction: direction/forward
top level: BBa_T9002
start: 81
end: 836
orientation: inline

BBa_K112806
type: ComponentDefinition
persistent identity: BBa K112806
display id: BBa_K112806
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [T4 endolysin]
creator: Jin Huh
created: 2008-10-31T12:00:00Z
modified: 2015-05-08T01:09:19Z
discontinued: false
dominant: true
group u list: _224_
m user id: 0
owner id: 3025
owning group id: 171
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The lysozyme from enterobacteria phage T4 degrades peptidoglycan layer.
mutable notes: High expression of lysozyme only is toxic to bacteria.
mutable provenance: Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=66503&to=66997&view=gbwithparts
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: T4 Endolysin
sequence: https://synbiohub.org/public/igem/BBa_K112806_sequence/1

T4 Endolysin
type: SequenceAnnotation
persistent identity: annotation1999018
display id: annotation1999018
version: 1
top level: BBa_K112806
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1

https://synbiohub.org/public/igem/BBa_K112806/annotation1999018/range1999018/1
type: Range
persistent identity: range1999018
display id: range1999018
version: 1
direction: direction/forward
top level: BBa_K112806
start: 17
end: 511
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0026
display id: BBa_E0026
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced cyan fluorescent protein derived from A. victoria GFP
creator: jcbraff
created: 2004-06-06T11:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_E0026_sequence/1

cheB
type: ComponentDefinition
persistent identity: BBa C0024
display id: BBa_C0024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CheB chemotaxis coding sequence (protein glutamate methylesterase)
creator: MIT SBC 2004 (Ken)
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: CheB is part of the chemotaxis pathway in E. coli.
mutable notes: Checked (DE, VC, KN). Part ready for synthesis. 8/3/4
mutable provenance: NCBI - E. Coli strain K12 substrain MG1655
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: CheB
sequence: https://synbiohub.org/public/igem/BBa_C0024_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214017
display id: annotation2214017
version: 1
top level: cheB
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0024/annotation2214017/range2214017/1

https://synbiohub.org/public/igem/BBa_C0024/annotation2214017/range2214017/1
type: Range
persistent identity: range2214017
display id: range2214017
version: 1
direction: direction/forward
top level: cheB
start: 1054
end: 1078
orientation: inline

CheB
type: SequenceAnnotation
persistent identity: annotation1891614
display id: annotation1891614
version: 1
top level: cheB
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0024/annotation1891614/range1891614/1

https://synbiohub.org/public/igem/BBa_C0024/annotation1891614/range1891614/1
type: Range
persistent identity: range1891614
display id: range1891614
version: 1
direction: direction/forward
top level: cheB
start: 1
end: 1053
orientation: inline

BBa_J24679
type: ComponentDefinition
persistent identity: BBa J24679
display id: BBa_J24679
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS + LacI
creator: Annie Gao
created: 2006-10-25T11:00:00Z
modified: 2015-08-31T04:08:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _58_
m user id: 0
owner id: 1017
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Adds an RBS to LacI protein coding region
mutable notes: Strength of RBS
mutable provenance: composite of other iGEM parts
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0033
component: BBa_C0012
sequence annotation: BBa_C0012
sequence annotation: BBa_B0033
sequence: https://synbiohub.org/public/igem/BBa_J24679_sequence/1

BBa_B0033
type: Component
persistent identity: component2243741
display id: component2243741
version: 1
top level: BBa_J24679
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_C0012
type: Component
persistent identity: component2243744
display id: component2243744
version: 1
top level: BBa_J24679
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation2243744
display id: annotation2243744
version: 1
top level: BBa_J24679
location: https://synbiohub.org/public/igem/BBa_J24679/annotation2243744/range2243744/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_J24679/annotation2243744/range2243744/1
type: Range
persistent identity: range2243744
display id: range2243744
version: 1
direction: direction/forward
top level: BBa_J24679
start: 18
end: 1145
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2243741
display id: annotation2243741
version: 1
top level: BBa_J24679
location: https://synbiohub.org/public/igem/BBa_J24679/annotation2243741/range2243741/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_J24679/annotation2243741/range2243741/1
type: Range
persistent identity: range2243741
display id: range2243741
version: 1
direction: direction/forward
top level: BBa_J24679
start: 1
end: 11
orientation: inline

BBa_I732020
type: ComponentDefinition
persistent identity: BBa I732020
display id: BBa_I732020
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lacZ alpha (fragment RBS+ TERM+)
creator: Zhan Jian
created: 2007-07-13T11:00:00Z
modified: 2015-08-31T04:07:56Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: None
mutable notes: None
mutable provenance: I732018 inserted into pSB1AK3-B0015
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_B0010
component: BBa_B0012
component: BBa_I732006
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I732006
sequence: https://synbiohub.org/public/igem/BBa_I732020_sequence/1

BBa_B0034
type: Component
persistent identity: component1938215
display id: component1938215
version: 1
top level: BBa_I732020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1938220
display id: component1938220
version: 1
top level: BBa_I732020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1938222
display id: component1938222
version: 1
top level: BBa_I732020
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_I732006
type: Component
persistent identity: component1938219
display id: component1938219
version: 1
top level: BBa_I732020
access: public
definition: lacZ-alpha

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1938215
display id: annotation1938215
version: 1
top level: BBa_I732020
location: https://synbiohub.org/public/igem/BBa_I732020/annotation1938215/range1938215/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732020/annotation1938215/range1938215/1
type: Range
persistent identity: range1938215
display id: range1938215
version: 1
direction: direction/reverse
top level: BBa_I732020
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1938222
display id: annotation1938222
version: 1
top level: BBa_I732020
location: https://synbiohub.org/public/igem/BBa_I732020/annotation1938222/range1938222/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732020/annotation1938222/range1938222/1
type: Range
persistent identity: range1938222
display id: range1938222
version: 1
direction: direction/forward
top level: BBa_I732020
start: 349
end: 389
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1938220
display id: annotation1938220
version: 1
top level: BBa_I732020
location: https://synbiohub.org/public/igem/BBa_I732020/annotation1938220/range1938220/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732020/annotation1938220/range1938220/1
type: Range
persistent identity: range1938220
display id: range1938220
version: 1
direction: direction/forward
top level: BBa_I732020
start: 261
end: 340
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation1938219
display id: annotation1938219
version: 1
top level: BBa_I732020
location: https://synbiohub.org/public/igem/BBa_I732020/annotation1938219/range1938219/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_I732020/annotation1938219/range1938219/1
type: Range
persistent identity: range1938219
display id: range1938219
version: 1
direction: direction/reverse
top level: BBa_I732020
start: 19
end: 252
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Pbad promoter
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: PBad promoter from I0500 without AraC.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K081016
type: ComponentDefinition
persistent identity: BBa K081016
display id: BBa_K081016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: lasI protein generator - PoPS->lasI
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: PoPS in -> lasI out. Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: lasI: T:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B1006
component: BBa_B0030
component: BBa_C0078
sequence annotation: BBa_B0030
sequence annotation: BBa_C0078
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081016_sequence/1

BBa_B1006
type: Component
persistent identity: component2246167
display id: component2246167
version: 1
top level: BBa_K081016
access: public
definition: BBa_B1006

BBa_B0030
type: Component
persistent identity: component2246157
display id: component2246157
version: 1
top level: BBa_K081016
access: public
definition: BBa_B0030

BBa_C0078
type: Component
persistent identity: component2246162
display id: component2246162
version: 1
top level: BBa_K081016
access: public
definition: lasI

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246157
display id: annotation2246157
version: 1
top level: BBa_K081016
location: https://synbiohub.org/public/igem/BBa_K081016/annotation2246157/range2246157/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081016/annotation2246157/range2246157/1
type: Range
persistent identity: range2246157
display id: range2246157
version: 1
direction: direction/forward
top level: BBa_K081016
start: 1
end: 15
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2246162
display id: annotation2246162
version: 1
top level: BBa_K081016
location: https://synbiohub.org/public/igem/BBa_K081016/annotation2246162/range2246162/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K081016/annotation2246162/range2246162/1
type: Range
persistent identity: range2246162
display id: range2246162
version: 1
direction: direction/reverse
top level: BBa_K081016
start: 22
end: 688
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2246167
display id: annotation2246167
version: 1
top level: BBa_K081016
location: https://synbiohub.org/public/igem/BBa_K081016/annotation2246167/range2246167/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081016/annotation2246167/range2246167/1
type: Range
persistent identity: range2246167
display id: range2246167
version: 1
direction: direction/reverse
top level: BBa_K081016
start: 697
end: 735
orientation: inline

BBa_I6088
type: ComponentDefinition
persistent identity: BBa I6088
display id: BBa_I6088
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter O_H Test I0500.E0432
creator: Randy Rettberg
created: 2004-04-21T11:00:00Z
modified: 2015-08-31T04:07:43Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0012
component: BBa_E0032
component: BBa_B0034
component: BBa_I0500
component: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I0500
sequence annotation: BBa_B0010
sequence annotation: BBa_E0032
sequence: https://synbiohub.org/public/igem/BBa_I6088_sequence/1

BBa_B0012
type: Component
persistent identity: component949669
display id: component949669
version: 1
top level: BBa_I6088
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0032
type: Component
persistent identity: component949651
display id: component949651
version: 1
top level: BBa_I6088
access: public
definition: https://synbiohub.org/public/igem/BBa_E0032/1

BBa_B0034
type: Component
persistent identity: component949639
display id: component949639
version: 1
top level: BBa_I6088
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I0500
type: Component
persistent identity: component949634
display id: component949634
version: 1
top level: BBa_I6088
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0010
type: Component
persistent identity: component949659
display id: component949659
version: 1
top level: BBa_I6088
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation949669
display id: annotation949669
version: 1
top level: BBa_I6088
location: https://synbiohub.org/public/igem/BBa_I6088/annotation949669/range949669/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6088/annotation949669/range949669/1
type: Range
persistent identity: range949669
display id: range949669
version: 1
direction: direction/forward
top level: BBa_I6088
start: 2095
end: 2135
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation949639
display id: annotation949639
version: 1
top level: BBa_I6088
location: https://synbiohub.org/public/igem/BBa_I6088/annotation949639/range949639/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I6088/annotation949639/range949639/1
type: Range
persistent identity: range949639
display id: range949639
version: 1
direction: direction/forward
top level: BBa_I6088
start: 1219
end: 1230
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation949634
display id: annotation949634
version: 1
top level: BBa_I6088
location: https://synbiohub.org/public/igem/BBa_I6088/annotation949634/range949634/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I6088/annotation949634/range949634/1
type: Range
persistent identity: range949634
display id: range949634
version: 1
direction: direction/forward
top level: BBa_I6088
start: 1
end: 1210
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation949659
display id: annotation949659
version: 1
top level: BBa_I6088
location: https://synbiohub.org/public/igem/BBa_I6088/annotation949659/range949659/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I6088/annotation949659/range949659/1
type: Range
persistent identity: range949659
display id: range949659
version: 1
direction: direction/forward
top level: BBa_I6088
start: 2007
end: 2086
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation949651
display id: annotation949651
version: 1
top level: BBa_I6088
location: https://synbiohub.org/public/igem/BBa_I6088/annotation949651/range949651/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I6088/annotation949651/range949651/1
type: Range
persistent identity: range949651
display id: range949651
version: 1
direction: direction/forward
top level: BBa_I6088
start: 1237
end: 1998
orientation: inline

BBa_I13604
type: ComponentDefinition
persistent identity: BBa I13604
display id: BBa_I13604
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Reporter construct for constitutive YFP and inducible CFP
creator: Chris Batton, Victoria Chou, Kenneth Nesmith
created: 2004-07-15T11:00:00Z
modified: 2015-08-31T04:07:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _6_
m user id: 0
owner id: 139
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Reporter Construct with Tet operator+YFP(LVA-) reporter followed by a Lac operator +CFP(LVA-) reporter
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I6032
component: BBa_E0420
component: BBa_R0011
sequence annotation: BBa_E0420
sequence annotation: BBa_I6032
sequence annotation: BBa_R0011
sequence: https://synbiohub.org/public/igem/BBa_I13604_sequence/1

BBa_I6032
type: Component
persistent identity: component2228609
display id: component2228609
version: 1
top level: BBa_I13604
access: public
definition: https://synbiohub.org/public/igem/BBa_I6032/1

BBa_E0420
type: Component
persistent identity: component2228624
display id: component2228624
version: 1
top level: BBa_I13604
access: public
definition: BBa_E0420

BBa_R0011
type: Component
persistent identity: component2228610
display id: component2228610
version: 1
top level: BBa_I13604
access: public
definition: https://synbiohub.org/public/igem/BBa_R0011/1

BBa_E0420
type: SequenceAnnotation
persistent identity: annotation2228624
display id: annotation2228624
version: 1
top level: BBa_I13604
location: https://synbiohub.org/public/igem/BBa_I13604/annotation2228624/range2228624/1
component: BBa_E0420

https://synbiohub.org/public/igem/BBa_I13604/annotation2228624/range2228624/1
type: Range
persistent identity: range2228624
display id: range2228624
version: 1
direction: direction/reverse
top level: BBa_I13604
start: 1012
end: 1889
orientation: inline

BBa_I6032
type: SequenceAnnotation
persistent identity: annotation2228609
display id: annotation2228609
version: 1
top level: BBa_I13604
location: https://synbiohub.org/public/igem/BBa_I13604/annotation2228609/range2228609/1
component: BBa_I6032

https://synbiohub.org/public/igem/BBa_I13604/annotation2228609/range2228609/1
type: Range
persistent identity: range2228609
display id: range2228609
version: 1
direction: direction/reverse
top level: BBa_I13604
start: 1
end: 940
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2228610
display id: annotation2228610
version: 1
top level: BBa_I13604
location: https://synbiohub.org/public/igem/BBa_I13604/annotation2228610/range2228610/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_I13604/annotation2228610/range2228610/1
type: Range
persistent identity: range2228610
display id: range2228610
version: 1
direction: direction/forward
top level: BBa_I13604
start: 949
end: 1002
orientation: inline

BBa_I732820
type: ComponentDefinition
persistent identity: BBa I732820
display id: BBa_I732820
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI (RBS+, TERM+)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_B0010
component: BBa_I732100
component: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I732100
sequence: https://synbiohub.org/public/igem/BBa_I732820_sequence/1

BBa_B0034
type: Component
persistent identity: component1946760
display id: component1946760
version: 1
top level: BBa_I732820
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1946762
display id: component1946762
version: 1
top level: BBa_I732820
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I732100
type: Component
persistent identity: component1946761
display id: component1946761
version: 1
top level: BBa_I732820
access: public
definition: LacI

BBa_B0012
type: Component
persistent identity: component1946764
display id: component1946764
version: 1
top level: BBa_I732820
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1946762
display id: annotation1946762
version: 1
top level: BBa_I732820
location: https://synbiohub.org/public/igem/BBa_I732820/annotation1946762/range1946762/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732820/annotation1946762/range1946762/1
type: Range
persistent identity: range1946762
display id: range1946762
version: 1
direction: direction/forward
top level: BBa_I732820
start: 1113
end: 1192
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1946764
display id: annotation1946764
version: 1
top level: BBa_I732820
location: https://synbiohub.org/public/igem/BBa_I732820/annotation1946764/range1946764/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732820/annotation1946764/range1946764/1
type: Range
persistent identity: range1946764
display id: range1946764
version: 1
direction: direction/forward
top level: BBa_I732820
start: 1201
end: 1241
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946760
display id: annotation1946760
version: 1
top level: BBa_I732820
location: https://synbiohub.org/public/igem/BBa_I732820/annotation1946760/range1946760/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732820/annotation1946760/range1946760/1
type: Range
persistent identity: range1946760
display id: range1946760
version: 1
direction: direction/reverse
top level: BBa_I732820
start: 1
end: 12
orientation: inline

BBa_I732100
type: SequenceAnnotation
persistent identity: annotation1946761
display id: annotation1946761
version: 1
top level: BBa_I732820
location: https://synbiohub.org/public/igem/BBa_I732820/annotation1946761/range1946761/1
component: BBa_I732100

https://synbiohub.org/public/igem/BBa_I732820/annotation1946761/range1946761/1
type: Range
persistent identity: range1946761
display id: range1946761
version: 1
direction: direction/reverse
top level: BBa_I732820
start: 19
end: 1104
orientation: inline

PelB
type: ComponentDefinition
persistent identity: BBa J32015
display id: BBa_J32015
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PelB leader sequence; directs protein to E. coli periplasmic membrane
creator: Austen Heinz
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
discontinued: false
dominant: true
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface.
mutable notes: .
mutable provenance: Novagen
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: PelB
sequence: https://synbiohub.org/public/igem/BBa_J32015_sequence/1

PelB
type: SequenceAnnotation
persistent identity: annotation1898182
display id: annotation1898182
version: 1
top level: PelB
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1

https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1
type: Range
persistent identity: range1898182
display id: range1898182
version: 1
direction: direction/forward
top level: PelB
start: 1
end: 66
orientation: inline

rhlR
type: ComponentDefinition
persistent identity: BBa C0071
display id: BBa_C0071
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rhlR repressor/activator from P. aeruginosa PA3477 (+LVA)
creator: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
created: 2004-01-26T12:00:00Z
modified: 2015-08-31T04:07:23Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Transcriptional regulator, in complex with N-butyryl-HSL, RhlR binds to the Rhl promoter
mutable notes: (1/28/04 Edit Jamboree) (0) No BB sites (1) ATG (2) TAATAA (3) Blast checks out (4) No codon changes (5) Maybe chassis compatability (check by experiment) (6) Codon usage not optimal (but looks OK) (7) ssrA tag added
mutable provenance: P. aeruginosa PA3477
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Help:Barcodes
sequence annotation: LVA
sequence annotation: rhlR
sequence: https://synbiohub.org/public/igem/BBa_C0071_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213995
display id: annotation2213995
version: 1
top level: rhlR
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1

https://synbiohub.org/public/igem/BBa_C0071/annotation2213995/range2213995/1
type: Range
persistent identity: range2213995
display id: range2213995
version: 1
direction: direction/forward
top level: rhlR
start: 763
end: 787
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306523
display id: annotation306523
version: 1
top level: rhlR
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1

https://synbiohub.org/public/igem/BBa_C0071/annotation306523/range306523/1
type: Range
persistent identity: range306523
display id: range306523
version: 1
direction: direction/forward
top level: rhlR
start: 724
end: 756
orientation: inline

rhlR
type: SequenceAnnotation
persistent identity: annotation301192
display id: annotation301192
version: 1
top level: rhlR
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1

https://synbiohub.org/public/igem/BBa_C0071/annotation301192/range301192/1
type: Range
persistent identity: range301192
display id: range301192
version: 1
direction: direction/forward
top level: rhlR
start: 1
end: 723
orientation: inline

BBa_I741018
type: ComponentDefinition
persistent identity: BBa I741018
display id: BBa_I741018
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Right facing promoter (for xylF) controlled by xylR and CRP-cAMP
creator: Lucien Weiss
created: 2007-06-20T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1763
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: potential looping feature possibly removed
mutable notes: removed RBS, left promoter, and several xylR binding sites
mutable provenance: ecoli k12 chromosome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: +1
sequence annotation: Promoter: xylFp
sequence annotation: CRP-cAMP duel regulator binding site
sequence annotation: xylR activator binding site
sequence: https://synbiohub.org/public/igem/BBa_I741018_sequence/1

+1
type: SequenceAnnotation
persistent identity: annotation1935202
display id: annotation1935202
version: 1
top level: BBa_I741018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935202/range1935202/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935202/range1935202/1
type: Range
persistent identity: range1935202
display id: range1935202
version: 1
direction: direction/forward
top level: BBa_I741018
start: 195
end: 195
orientation: inline

Promoter: xylFp
type: SequenceAnnotation
persistent identity: annotation1935200
display id: annotation1935200
version: 1
top level: BBa_I741018
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935200/range1935200/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935200/range1935200/1
type: Range
persistent identity: range1935200
display id: range1935200
version: 1
direction: direction/forward
top level: BBa_I741018
start: 138
end: 215
orientation: inline

CRP-cAMP duel regulator binding site
type: SequenceAnnotation
persistent identity: annotation1935203
display id: annotation1935203
version: 1
top level: BBa_I741018
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935203/range1935203/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935203/range1935203/1
type: Range
persistent identity: range1935203
display id: range1935203
version: 1
direction: direction/forward
top level: BBa_I741018
start: 4
end: 25
orientation: inline

xylR activator binding site
type: SequenceAnnotation
persistent identity: annotation1935201
display id: annotation1935201
version: 1
top level: BBa_I741018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935201/range1935201/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935201/range1935201/1
type: Range
persistent identity: range1935201
display id: range1935201
version: 1
direction: direction/forward
top level: BBa_I741018
start: 146
end: 162
orientation: inline

xylR activator binding site
type: SequenceAnnotation
persistent identity: annotation1935199
display id: annotation1935199
version: 1
top level: BBa_I741018
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I741018/annotation1935199/range1935199/1

https://synbiohub.org/public/igem/BBa_I741018/annotation1935199/range1935199/1
type: Range
persistent identity: range1935199
display id: range1935199
version: 1
direction: direction/forward
top level: BBa_I741018
start: 125
end: 142
orientation: inline

BBa_I732821
type: ComponentDefinition
persistent identity: BBa I732821
display id: BBa_I732821
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LRLa (RBS+, TERM+)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I732101
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_I732101
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732821_sequence/1

BBa_I732101
type: Component
persistent identity: component1946733
display id: component1946733
version: 1
top level: BBa_I732821
access: public
definition: LRLa

BBa_B0012
type: Component
persistent identity: component1946736
display id: component1946736
version: 1
top level: BBa_I732821
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1946734
display id: component1946734
version: 1
top level: BBa_I732821
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1946732
display id: component1946732
version: 1
top level: BBa_I732821
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1946736
display id: annotation1946736
version: 1
top level: BBa_I732821
location: https://synbiohub.org/public/igem/BBa_I732821/annotation1946736/range1946736/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732821/annotation1946736/range1946736/1
type: Range
persistent identity: range1946736
display id: range1946736
version: 1
direction: direction/forward
top level: BBa_I732821
start: 1201
end: 1241
orientation: inline

BBa_I732101
type: SequenceAnnotation
persistent identity: annotation1946733
display id: annotation1946733
version: 1
top level: BBa_I732821
location: https://synbiohub.org/public/igem/BBa_I732821/annotation1946733/range1946733/1
component: BBa_I732101

https://synbiohub.org/public/igem/BBa_I732821/annotation1946733/range1946733/1
type: Range
persistent identity: range1946733
display id: range1946733
version: 1
direction: direction/reverse
top level: BBa_I732821
start: 19
end: 1104
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1946734
display id: annotation1946734
version: 1
top level: BBa_I732821
location: https://synbiohub.org/public/igem/BBa_I732821/annotation1946734/range1946734/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732821/annotation1946734/range1946734/1
type: Range
persistent identity: range1946734
display id: range1946734
version: 1
direction: direction/forward
top level: BBa_I732821
start: 1113
end: 1192
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946732
display id: annotation1946732
version: 1
top level: BBa_I732821
location: https://synbiohub.org/public/igem/BBa_I732821/annotation1946732/range1946732/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732821/annotation1946732/range1946732/1
type: Range
persistent identity: range1946732
display id: range1946732
version: 1
direction: direction/reverse
top level: BBa_I732821
start: 1
end: 12
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa I732100
display id: BBa_I732100
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LacI
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2016-10-18T06:58:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 31248
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I732100_sequence/1

BBa_K116640
type: ComponentDefinition
persistent identity: BBa K116640
display id: BBa_K116640
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pTet + RBS + LuxR + T
creator: Jesse Wu
created: 2008-10-30T12:00:00Z
modified: 2015-05-08T01:09:34Z
discontinued: false
dominant: false
group u list: _210_
m user id: 0
owner id: 2749
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: LuxR regulated by the pTet promoter. Used to constitutively express LuxR.
mutable notes: none
mutable provenance: ligation
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K116626
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_K116626
sequence: https://synbiohub.org/public/igem/BBa_K116640_sequence/1

BBa_K116626
type: Component
persistent identity: component2254978
display id: component2254978
version: 1
top level: BBa_K116640
access: public
definition: https://synbiohub.org/public/igem/BBa_K116626/1

BBa_R0040
type: Component
persistent identity: component2254956
display id: component2254956
version: 1
top level: BBa_K116640
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2254956
display id: annotation2254956
version: 1
top level: BBa_K116640
location: https://synbiohub.org/public/igem/BBa_K116640/annotation2254956/range2254956/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K116640/annotation2254956/range2254956/1
type: Range
persistent identity: range2254956
display id: range2254956
version: 1
direction: direction/forward
top level: BBa_K116640
start: 1
end: 54
orientation: inline

BBa_K116626
type: SequenceAnnotation
persistent identity: annotation2254978
display id: annotation2254978
version: 1
top level: BBa_K116640
location: https://synbiohub.org/public/igem/BBa_K116640/annotation2254978/range2254978/1
component: BBa_K116626

https://synbiohub.org/public/igem/BBa_K116640/annotation2254978/range2254978/1
type: Range
persistent identity: range2254978
display id: range2254978
version: 1
direction: direction/reverse
top level: BBa_K116640
start: 63
end: 999
orientation: inline

BBa_K137040
type: ComponentDefinition
persistent identity: BBa K137040
display id: BBa_K137040
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Coding SSM device: GFP with (AGTC)10 after start codon
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Strong promoter + strong RBS + GFP with (AGTC)9 repeat after start codon + double terminator
mutable notes: Strong promoter + strong RBS + GFP with (AGTC)9 repeat after start codon + double terminator
mutable provenance: S03952 + S03996
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23100
component: BBa_B0012
component: BBa_B0034
component: BBa_B0010
component: BBa_K137027
sequence annotation: BBa_B0012
sequence annotation: BBa_K137027
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K137040_sequence/1

BBa_J23100
type: Component
persistent identity: component1967945
display id: component1967945
version: 1
top level: BBa_K137040
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0012
type: Component
persistent identity: component1967952
display id: component1967952
version: 1
top level: BBa_K137040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0034
type: Component
persistent identity: component1967947
display id: component1967947
version: 1
top level: BBa_K137040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1967950
display id: component1967950
version: 1
top level: BBa_K137040
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K137027
type: Component
persistent identity: component1967949
display id: component1967949
version: 1
top level: BBa_K137040
access: public
definition: https://synbiohub.org/public/igem/BBa_K137027/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1967952
display id: annotation1967952
version: 1
top level: BBa_K137040
location: https://synbiohub.org/public/igem/BBa_K137040/annotation1967952/range1967952/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137040/annotation1967952/range1967952/1
type: Range
persistent identity: range1967952
display id: range1967952
version: 1
direction: direction/forward
top level: BBa_K137040
start: 918
end: 958
orientation: inline

BBa_K137027
type: SequenceAnnotation
persistent identity: annotation1967949
display id: annotation1967949
version: 1
top level: BBa_K137040
location: https://synbiohub.org/public/igem/BBa_K137040/annotation1967949/range1967949/1
component: BBa_K137027

https://synbiohub.org/public/igem/BBa_K137040/annotation1967949/range1967949/1
type: Range
persistent identity: range1967949
display id: range1967949
version: 1
direction: direction/reverse
top level: BBa_K137040
start: 62
end: 821
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1967950
display id: annotation1967950
version: 1
top level: BBa_K137040
location: https://synbiohub.org/public/igem/BBa_K137040/annotation1967950/range1967950/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137040/annotation1967950/range1967950/1
type: Range
persistent identity: range1967950
display id: range1967950
version: 1
direction: direction/forward
top level: BBa_K137040
start: 830
end: 909
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1967947
display id: annotation1967947
version: 1
top level: BBa_K137040
location: https://synbiohub.org/public/igem/BBa_K137040/annotation1967947/range1967947/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137040/annotation1967947/range1967947/1
type: Range
persistent identity: range1967947
display id: range1967947
version: 1
direction: direction/reverse
top level: BBa_K137040
start: 44
end: 55
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1967945
display id: annotation1967945
version: 1
top level: BBa_K137040
location: https://synbiohub.org/public/igem/BBa_K137040/annotation1967945/range1967945/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K137040/annotation1967945/range1967945/1
type: Range
persistent identity: range1967945
display id: range1967945
version: 1
direction: direction/reverse
top level: BBa_K137040
start: 1
end: 35
orientation: inline

BBa_K228001
type: ComponentDefinition
persistent identity: BBa K228001
display id: BBa_K228001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: SupD-tRNA
creator: Lin Min
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated. This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.
mutable notes: There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.
mutable provenance: SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K228001_sequence/1

BBa_I746001
type: ComponentDefinition
persistent identity: BBa I746001
display id: BBa_I746001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: I746000 (AIP generator - agrB + agrD with RBSes) + B0015 (Terminator)
creator: Lovelace Soirez
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:04Z
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2127
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part is simply the AIP generator from the agr system with a standard registry terminator downstream. The original (I746000) part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. This part contains the coding regions for group I agrB and agrD (in that order) from S. aureus strain NCTC8325, prefixed by the B0034 RBS and separated by a SaII site.
mutable notes: N/A
mutable provenance: The original sequences for agrB and agrD were taken from the S. aureus Sequencing Project at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0010
component: BBa_I746000
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence annotation: BBa_I746000
sequence: https://synbiohub.org/public/igem/BBa_I746001_sequence/1

BBa_B0010
type: Component
persistent identity: component1952601
display id: component1952601
version: 1
top level: BBa_I746001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I746000
type: Component
persistent identity: component1952600
display id: component1952600
version: 1
top level: BBa_I746001
access: public
definition: https://synbiohub.org/public/igem/BBa_I746000/1

BBa_B0012
type: Component
persistent identity: component1952603
display id: component1952603
version: 1
top level: BBa_I746001
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1952603
display id: annotation1952603
version: 1
top level: BBa_I746001
location: https://synbiohub.org/public/igem/BBa_I746001/annotation1952603/range1952603/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746001/annotation1952603/range1952603/1
type: Range
persistent identity: range1952603
display id: range1952603
version: 1
direction: direction/forward
top level: BBa_I746001
start: 856
end: 896
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1952601
display id: annotation1952601
version: 1
top level: BBa_I746001
location: https://synbiohub.org/public/igem/BBa_I746001/annotation1952601/range1952601/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746001/annotation1952601/range1952601/1
type: Range
persistent identity: range1952601
display id: range1952601
version: 1
direction: direction/forward
top level: BBa_I746001
start: 768
end: 847
orientation: inline

BBa_I746000
type: SequenceAnnotation
persistent identity: annotation1952600
display id: annotation1952600
version: 1
top level: BBa_I746001
location: https://synbiohub.org/public/igem/BBa_I746001/annotation1952600/range1952600/1
component: BBa_I746000

https://synbiohub.org/public/igem/BBa_I746001/annotation1952600/range1952600/1
type: Range
persistent identity: range1952600
display id: range1952600
version: 1
direction: direction/reverse
top level: BBa_I746001
start: 1
end: 759
orientation: inline

BBa_I715039
type: ComponentDefinition
persistent identity: BBa I715039
display id: BBa_I715039
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: pLac-RBS-RFP
creator: Oyinade Adefuye
created: 2007-07-31T11:00:00Z
modified: 2015-08-31T04:07:49Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1523
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RBS-1Chlora
mutable notes: RBS-1Chlora
mutable provenance: RBS-1Chlora
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13502
component: BBa_R0010
sequence annotation: BBa_R0010
sequence annotation: BBa_I13502
sequence: https://synbiohub.org/public/igem/BBa_I715039_sequence/1

BBa_I13502
type: Component
persistent identity: component2245609
display id: component2245609
version: 1
top level: BBa_I715039
access: public
definition: mRFP1

BBa_R0010
type: Component
persistent identity: component2245597
display id: component2245597
version: 1
top level: BBa_I715039
access: public
definition: LacI

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2245597
display id: annotation2245597
version: 1
top level: BBa_I715039
location: https://synbiohub.org/public/igem/BBa_I715039/annotation2245597/range2245597/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I715039/annotation2245597/range2245597/1
type: Range
persistent identity: range2245597
display id: range2245597
version: 1
direction: direction/forward
top level: BBa_I715039
start: 1
end: 200
orientation: inline

BBa_I13502
type: SequenceAnnotation
persistent identity: annotation2245609
display id: annotation2245609
version: 1
top level: BBa_I715039
location: https://synbiohub.org/public/igem/BBa_I715039/annotation2245609/range2245609/1
component: BBa_I13502

https://synbiohub.org/public/igem/BBa_I715039/annotation2245609/range2245609/1
type: Range
persistent identity: range2245609
display id: range2245609
version: 1
direction: direction/reverse
top level: BBa_I715039
start: 209
end: 932
orientation: inline

BBa_P0340
type: ComponentDefinition
persistent identity: BBa P0340
display id: BBa_P0340
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> TetR [S0157]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_B0010
component: BBa_B0012
component: BBa_B0033
sequence annotation: BBa_B0012
sequence annotation: BBa_B0033
sequence annotation: BBa_B0010
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_P0340_sequence/1

BBa_C0040
type: Component
persistent identity: component944653
display id: component944653
version: 1
top level: BBa_P0340
access: public
definition: https://synbiohub.org/public/igem/BBa_C0040/1

BBa_B0010
type: Component
persistent identity: component944659
display id: component944659
version: 1
top level: BBa_P0340
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component944669
display id: component944669
version: 1
top level: BBa_P0340
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0033
type: Component
persistent identity: component944642
display id: component944642
version: 1
top level: BBa_P0340
access: public
definition: https://synbiohub.org/public/igem/BBa_B0033/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944669
display id: annotation944669
version: 1
top level: BBa_P0340
location: https://synbiohub.org/public/igem/BBa_P0340/annotation944669/range944669/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0340/annotation944669/range944669/1
type: Range
persistent identity: range944669
display id: range944669
version: 1
direction: direction/forward
top level: BBa_P0340
start: 799
end: 839
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation944642
display id: annotation944642
version: 1
top level: BBa_P0340
location: https://synbiohub.org/public/igem/BBa_P0340/annotation944642/range944642/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_P0340/annotation944642/range944642/1
type: Range
persistent identity: range944642
display id: range944642
version: 1
direction: direction/forward
top level: BBa_P0340
start: 1
end: 11
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944659
display id: annotation944659
version: 1
top level: BBa_P0340
location: https://synbiohub.org/public/igem/BBa_P0340/annotation944659/range944659/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0340/annotation944659/range944659/1
type: Range
persistent identity: range944659
display id: range944659
version: 1
direction: direction/forward
top level: BBa_P0340
start: 711
end: 790
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944653
display id: annotation944653
version: 1
top level: BBa_P0340
location: https://synbiohub.org/public/igem/BBa_P0340/annotation944653/range944653/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0340/annotation944653/range944653/1
type: Range
persistent identity: range944653
display id: range944653
version: 1
direction: direction/forward
top level: BBa_P0340
start: 18
end: 677
orientation: inline

lox
type: ComponentDefinition
persistent identity: BBa J61046
display id: BBa_J61046
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: [Lox] site for recombination
creator: John Anderson
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: bob
mutable notes: bob
mutable provenance: bob
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J61046_sequence/1

BBa_K098991
type: ComponentDefinition
persistent identity: BBa K098991
display id: BBa_K098991
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI promoter with GFP reporter
creator: Harvard iGEM 08
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:41Z
discontinued: false
dominant: false
experience: experience/None
group u list: _196_
m user id: 0
owner id: 3394
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the a constitutive GFP reporter driven by the cI promoter.
mutable notes: Obtained by cloning.
mutable provenance: Biobricks.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_E0040
component: BBa_R0051
component: BBa_B0010
component: BBa_B0032
component: BBa_B0012
sequence annotation: BBa_R0051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0032
sequence: https://synbiohub.org/public/igem/BBa_K098991_sequence/1

BBa_E0040
type: Component
persistent identity: component1989547
display id: component1989547
version: 1
top level: BBa_K098991
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_R0051
type: Component
persistent identity: component1989539
display id: component1989539
version: 1
top level: BBa_K098991
access: public
definition: https://synbiohub.org/public/igem/BBa_R0051/1

BBa_B0010
type: Component
persistent identity: component1989548
display id: component1989548
version: 1
top level: BBa_K098991
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0032
type: Component
persistent identity: component1989544
display id: component1989544
version: 1
top level: BBa_K098991
access: public
definition: https://synbiohub.org/public/igem/BBa_B0032/1

BBa_B0012
type: Component
persistent identity: component1989550
display id: component1989550
version: 1
top level: BBa_K098991
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1989539
display id: annotation1989539
version: 1
top level: BBa_K098991
location: https://synbiohub.org/public/igem/BBa_K098991/annotation1989539/range1989539/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K098991/annotation1989539/range1989539/1
type: Range
persistent identity: range1989539
display id: range1989539
version: 1
direction: direction/forward
top level: BBa_K098991
start: 1
end: 49
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1989548
display id: annotation1989548
version: 1
top level: BBa_K098991
location: https://synbiohub.org/public/igem/BBa_K098991/annotation1989548/range1989548/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K098991/annotation1989548/range1989548/1
type: Range
persistent identity: range1989548
display id: range1989548
version: 1
direction: direction/forward
top level: BBa_K098991
start: 805
end: 884
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1989550
display id: annotation1989550
version: 1
top level: BBa_K098991
location: https://synbiohub.org/public/igem/BBa_K098991/annotation1989550/range1989550/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K098991/annotation1989550/range1989550/1
type: Range
persistent identity: range1989550
display id: range1989550
version: 1
direction: direction/forward
top level: BBa_K098991
start: 893
end: 933
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1989547
display id: annotation1989547
version: 1
top level: BBa_K098991
location: https://synbiohub.org/public/igem/BBa_K098991/annotation1989547/range1989547/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K098991/annotation1989547/range1989547/1
type: Range
persistent identity: range1989547
display id: range1989547
version: 1
direction: direction/reverse
top level: BBa_K098991
start: 77
end: 796
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1989544
display id: annotation1989544
version: 1
top level: BBa_K098991
location: https://synbiohub.org/public/igem/BBa_K098991/annotation1989544/range1989544/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K098991/annotation1989544/range1989544/1
type: Range
persistent identity: range1989544
display id: range1989544
version: 1
direction: direction/forward
top level: BBa_K098991
start: 58
end: 70
orientation: inline

RQE Lambda
type: ComponentDefinition
persistent identity: BBa I744204
display id: BBa_I744204
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Tc sensor and cl lambda generator (Tet promoter)
creator: Thomas Segall-Shapiro
created: 2007-07-17T11:00:00Z
modified: 2015-08-31T04:08:03Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _147_
m user id: 0
owner id: 728
owning group id: 75
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This part serves as a sensor and reporter of intracellular tetracycline concentrations as well as producing cl (lambda) corrosponding to the level of Tc. This part uses the tet promoter to drive production of TetR. The sensor and reporter portions are identical to Part:BBa_I744104.
mutable notes: The promoter driving tetR production is crucial to the sensor's sensitivity and range of detection.
mutable provenance: Composite - all parts are drawn from the registry
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_Q04400
component: BBa_P0455
component: BBa_R0040
component: BBa_E0430
sequence annotation: BBa_R0040
sequence annotation: BBa_P0455
sequence annotation: BBa_E0430
sequence annotation: BBa_Q04400
sequence: https://synbiohub.org/public/igem/BBa_I744204_sequence/1

BBa_Q04400
type: Component
persistent identity: component2304719
display id: component2304719
version: 1
top level: RQE Lambda
access: public
definition: https://synbiohub.org/public/igem/BBa_Q04400/1

BBa_P0455
type: Component
persistent identity: component2304747
display id: component2304747
version: 1
top level: RQE Lambda
access: public
definition: https://synbiohub.org/public/igem/BBa_P0455/1

BBa_R0040
type: Component
persistent identity: component2304730
display id: component2304730
version: 1
top level: RQE Lambda
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_R0040
type: Component
persistent identity: component2304697
display id: component2304697
version: 1
top level: RQE Lambda
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_E0430
type: Component
persistent identity: component2304729
display id: component2304729
version: 1
top level: RQE Lambda
access: public
definition: BBa_E0430

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2304697
display id: annotation2304697
version: 1
top level: RQE Lambda
location: https://synbiohub.org/public/igem/BBa_I744204/annotation2304697/range2304697/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I744204/annotation2304697/range2304697/1
type: Range
persistent identity: range2304697
display id: range2304697
version: 1
direction: direction/forward
top level: RQE Lambda
start: 1
end: 54
orientation: inline

BBa_P0455
type: SequenceAnnotation
persistent identity: annotation2304747
display id: annotation2304747
version: 1
top level: RQE Lambda
location: https://synbiohub.org/public/igem/BBa_I744204/annotation2304747/range2304747/1
component: BBa_P0455

https://synbiohub.org/public/igem/BBa_I744204/annotation2304747/range2304747/1
type: Range
persistent identity: range2304747
display id: range2304747
version: 1
direction: direction/reverse
top level: RQE Lambda
start: 1921
end: 2816
orientation: inline

BBa_E0430
type: SequenceAnnotation
persistent identity: annotation2304729
display id: annotation2304729
version: 1
top level: RQE Lambda
location: https://synbiohub.org/public/igem/BBa_I744204/annotation2304729/range2304729/1
component: BBa_E0430

https://synbiohub.org/public/igem/BBa_I744204/annotation2304729/range2304729/1
type: Range
persistent identity: range2304729
display id: range2304729
version: 1
direction: direction/reverse
top level: RQE Lambda
start: 973
end: 1850
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2304730
display id: annotation2304730
version: 1
top level: RQE Lambda
location: https://synbiohub.org/public/igem/BBa_I744204/annotation2304730/range2304730/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I744204/annotation2304730/range2304730/1
type: Range
persistent identity: range2304730
display id: range2304730
version: 1
direction: direction/forward
top level: RQE Lambda
start: 1859
end: 1912
orientation: inline

BBa_Q04400
type: SequenceAnnotation
persistent identity: annotation2304719
display id: annotation2304719
version: 1
top level: RQE Lambda
location: https://synbiohub.org/public/igem/BBa_I744204/annotation2304719/range2304719/1
component: BBa_Q04400

https://synbiohub.org/public/igem/BBa_I744204/annotation2304719/range2304719/1
type: Range
persistent identity: range2304719
display id: range2304719
version: 1
direction: direction/reverse
top level: RQE Lambda
start: 63
end: 964
orientation: inline

BBa_S03653
type: ComponentDefinition
persistent identity: BBa S03653
display id: BBa_S03653
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: E0042:S03650
creator: Heather Keller
created: 2007-03-05T12:00:00Z
modified: 2015-05-08T01:14:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _10_
m user id: 0
owner id: 571
owning group id: 10
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0010
component: BBa_B0101
component: BBa_B0012
component: BBa_E0042
sequence annotation: BBa_B0101
sequence annotation: BBa_B0010
sequence annotation: BBa_E0042
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S03653_sequence/1

BBa_B0010
type: Component
persistent identity: component1920503
display id: component1920503
version: 1
top level: BBa_S03653
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0101
type: Component
persistent identity: component1920502
display id: component1920502
version: 1
top level: BBa_S03653
access: public
definition: https://synbiohub.org/public/igem/BBa_B0101/1

BBa_B0012
type: Component
persistent identity: component1920505
display id: component1920505
version: 1
top level: BBa_S03653
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0042
type: Component
persistent identity: component1920500
display id: component1920500
version: 1
top level: BBa_S03653
access: public
definition: https://synbiohub.org/public/igem/BBa_E0042/1

BBa_B0101
type: SequenceAnnotation
persistent identity: annotation1920502
display id: annotation1920502
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920502/range1920502/1
component: BBa_B0101

https://synbiohub.org/public/igem/BBa_S03653/annotation1920502/range1920502/1
type: Range
persistent identity: range1920502
display id: range1920502
version: 1
direction: direction/reverse
top level: BBa_S03653
start: 731
end: 788
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1920503
display id: annotation1920503
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920503/range1920503/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03653/annotation1920503/range1920503/1
type: Range
persistent identity: range1920503
display id: range1920503
version: 1
direction: direction/forward
top level: BBa_S03653
start: 797
end: 876
orientation: inline

BBa_E0042
type: SequenceAnnotation
persistent identity: annotation1920500
display id: annotation1920500
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920500/range1920500/1
component: BBa_E0042

https://synbiohub.org/public/igem/BBa_S03653/annotation1920500/range1920500/1
type: Range
persistent identity: range1920500
display id: range1920500
version: 1
direction: direction/reverse
top level: BBa_S03653
start: 1
end: 722
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1920505
display id: annotation1920505
version: 1
top level: BBa_S03653
location: https://synbiohub.org/public/igem/BBa_S03653/annotation1920505/range1920505/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03653/annotation1920505/range1920505/1
type: Range
persistent identity: range1920505
display id: range1920505
version: 1
direction: direction/forward
top level: BBa_S03653
start: 885
end: 925
orientation: inline

BBa_I732830
type: ComponentDefinition
persistent identity: BBa I732830
display id: BBa_I732830
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0203 (RBS+, TERM+)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/None
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_I732110
sequence annotation: BBa_B0012
sequence annotation: BBa_I732110
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I732830_sequence/1

BBa_B0034
type: Component
persistent identity: component1946832
display id: component1946832
version: 1
top level: BBa_I732830
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component1946836
display id: component1946836
version: 1
top level: BBa_I732830
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1946834
display id: component1946834
version: 1
top level: BBa_I732830
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_I732110
type: Component
persistent identity: component1946833
display id: component1946833
version: 1
top level: BBa_I732830
access: public
definition: ARL2A0203

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1946836
display id: annotation1946836
version: 1
top level: BBa_I732830
location: https://synbiohub.org/public/igem/BBa_I732830/annotation1946836/range1946836/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I732830/annotation1946836/range1946836/1
type: Range
persistent identity: range1946836
display id: range1946836
version: 1
direction: direction/forward
top level: BBa_I732830
start: 1201
end: 1241
orientation: inline

BBa_I732110
type: SequenceAnnotation
persistent identity: annotation1946833
display id: annotation1946833
version: 1
top level: BBa_I732830
location: https://synbiohub.org/public/igem/BBa_I732830/annotation1946833/range1946833/1
component: BBa_I732110

https://synbiohub.org/public/igem/BBa_I732830/annotation1946833/range1946833/1
type: Range
persistent identity: range1946833
display id: range1946833
version: 1
direction: direction/reverse
top level: BBa_I732830
start: 19
end: 1104
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946832
display id: annotation1946832
version: 1
top level: BBa_I732830
location: https://synbiohub.org/public/igem/BBa_I732830/annotation1946832/range1946832/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732830/annotation1946832/range1946832/1
type: Range
persistent identity: range1946832
display id: range1946832
version: 1
direction: direction/reverse
top level: BBa_I732830
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1946834
display id: annotation1946834
version: 1
top level: BBa_I732830
location: https://synbiohub.org/public/igem/BBa_I732830/annotation1946834/range1946834/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I732830/annotation1946834/range1946834/1
type: Range
persistent identity: range1946834
display id: range1946834
version: 1
direction: direction/forward
top level: BBa_I732830
start: 1113
end: 1192
orientation: inline

BBa_S03724
type: ComponentDefinition
persistent identity: BBa S03724
display id: BBa_S03724
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0015:S03708
creator: Josh Michener
created: 2007-06-25T11:00:00Z
modified: 2015-05-08T01:14:29Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _115_6_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_S03708
component: BBa_B0015
sequence annotation: BBa_S03708
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_S03724_sequence/1

BBa_S03708
type: Component
persistent identity: component2265453
display id: component2265453
version: 1
top level: BBa_S03724
access: public
definition: https://synbiohub.org/public/igem/BBa_S03708/1

BBa_B0015
type: Component
persistent identity: component2265438
display id: component2265438
version: 1
top level: BBa_S03724
access: public
definition: BBa_B0015

BBa_S03708
type: SequenceAnnotation
persistent identity: annotation2265453
display id: annotation2265453
version: 1
top level: BBa_S03724
location: https://synbiohub.org/public/igem/BBa_S03724/annotation2265453/range2265453/1
component: BBa_S03708

https://synbiohub.org/public/igem/BBa_S03724/annotation2265453/range2265453/1
type: Range
persistent identity: range2265453
display id: range2265453
version: 1
direction: direction/reverse
top level: BBa_S03724
start: 138
end: 1020
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2265438
display id: annotation2265438
version: 1
top level: BBa_S03724
location: https://synbiohub.org/public/igem/BBa_S03724/annotation2265438/range2265438/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S03724/annotation2265438/range2265438/1
type: Range
persistent identity: range2265438
display id: range2265438
version: 1
direction: direction/reverse
top level: BBa_S03724
start: 1
end: 129
orientation: inline

BBa_K200008
type: ComponentDefinition
persistent identity: BBa K200008
display id: BBa_K200008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Phenylalanine hydroxylase
creator: Charles Fracchia, Royah Vaezi, Tianyi Wang, Nuri Purswani
created: 2009-08-11T11:00:00Z
modified: 2015-05-08T01:11:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _298_
m user id: 0
owner id: 5091
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Phenylalanine hydroxylase (PAH) is an enzyme used to break down phenylalanine in humans.
mutable notes: none
mutable provenance: Homo Sapiens.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Reverse primer
sequence annotation: PAH
sequence annotation: Forward primer
sequence: https://synbiohub.org/public/igem/BBa_K200008_sequence/1

Reverse primer
type: SequenceAnnotation
persistent identity: annotation2018264
display id: annotation2018264
version: 1
top level: BBa_K200008
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K200008/annotation2018264/range2018264/1

https://synbiohub.org/public/igem/BBa_K200008/annotation2018264/range2018264/1
type: Range
persistent identity: range2018264
display id: range2018264
version: 1
direction: direction/reverse
top level: BBa_K200008
start: 1341
end: 1362
orientation: inline

PAH
type: SequenceAnnotation
persistent identity: annotation2018160
display id: annotation2018160
version: 1
top level: BBa_K200008
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K200008/annotation2018160/range2018160/1

https://synbiohub.org/public/igem/BBa_K200008/annotation2018160/range2018160/1
type: Range
persistent identity: range2018160
display id: range2018160
version: 1
direction: direction/forward
top level: BBa_K200008
start: 1
end: 3
orientation: inline

Forward primer
type: SequenceAnnotation
persistent identity: annotation2018253
display id: annotation2018253
version: 1
top level: BBa_K200008
role: feature/primer binding
role: SO:0005850
location: https://synbiohub.org/public/igem/BBa_K200008/annotation2018253/range2018253/1

https://synbiohub.org/public/igem/BBa_K200008/annotation2018253/range2018253/1
type: Range
persistent identity: range2018253
display id: range2018253
version: 1
direction: direction/forward
top level: BBa_K200008
start: 1
end: 16
orientation: inline

PAH
type: SequenceAnnotation
persistent identity: annotation2018159
display id: annotation2018159
version: 1
top level: BBa_K200008
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_K200008/annotation2018159/range2018159/1

https://synbiohub.org/public/igem/BBa_K200008/annotation2018159/range2018159/1
type: Range
persistent identity: range2018159
display id: range2018159
version: 1
direction: direction/forward
top level: BBa_K200008
start: 1360
end: 1362
orientation: inline

PAH
type: SequenceAnnotation
persistent identity: annotation2018158
display id: annotation2018158
version: 1
top level: BBa_K200008
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K200008/annotation2018158/range2018158/1

https://synbiohub.org/public/igem/BBa_K200008/annotation2018158/range2018158/1
type: Range
persistent identity: range2018158
display id: range2018158
version: 1
direction: direction/forward
top level: BBa_K200008
start: 1
end: 1362
orientation: inline

OriTR
type: ComponentDefinition
persistent identity: BBa J01003
display id: BBa_J01003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: OriT-R (Origin of transfer for the R-plasmid nic region)
creator: Golden Bear
created: 2005-10-17T11:00:00Z
modified: 2015-08-31T04:08:11Z
discontinued: false
dominant: false
experience: experience/None
group u list: _13_
m user id: 0
owner id: 395
owning group id: 13
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: OriTR, the R plasmid nic region, is where the relaxosome nicks the plasmid and conjugative transfer by R plasmid machinery begins.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Conjugation
role: SO:0000724
sequence: https://synbiohub.org/public/igem/BBa_J01003_sequence/1

BBa_K165002
type: ComponentDefinition
persistent identity: BBa K165002
display id: BBa_K165002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Kozak sequence (yeast RBS)
creator: John Szymanski
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:10:55Z
discontinued: false
dominant: false
experience: experience/None
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region.
mutable notes: -
mutable provenance: -
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K165002_sequence/1

difF
type: ComponentDefinition
persistent identity: BBa I742101
display id: BBa_I742101
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dif site with forward orientation
creator: Xiaonan Wang
created: 2007-08-07T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1965
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: 'dif' (division induced filamentation) is the recombination site for XerC and XerD recombinases. Once replicated, aligned direct repeats of the dif site are vital to DNA monomerisation during bacterial septation. As with other recombinase sites, inverted repeats cause inversion of the DNA in between. However, XerCD requires activation by FtsK and thus two dif-sites (see also part BBa_I742102) enables recombination that is temporally isolated to septation.
mutable notes: Constructed as two complimentary oligonucleotides.
mutable provenance: E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: SacI
sequence annotation: dif site (forward)
sequence: https://synbiohub.org/public/igem/BBa_I742101_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1940992
display id: annotation1940992
version: 1
top level: difF
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I742101/annotation1940992/range1940992/1

https://synbiohub.org/public/igem/BBa_I742101/annotation1940992/range1940992/1
type: Range
persistent identity: range1940992
display id: range1940992
version: 1
direction: direction/forward
top level: difF
start: 1
end: 3
orientation: inline

dif site (forward)
type: SequenceAnnotation
persistent identity: annotation1955264
display id: annotation1955264
version: 1
top level: difF
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I742101/annotation1955264/range1955264/1

https://synbiohub.org/public/igem/BBa_I742101/annotation1955264/range1955264/1
type: Range
persistent identity: range1955264
display id: range1955264
version: 1
direction: direction/forward
top level: difF
start: 4
end: 31
orientation: inline

BBa_K105024
type: ComponentDefinition
persistent identity: BBa K105024
display id: BBa_K105024
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Gal4 - operator
creator: Katja Karstens
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:08:52Z
discontinued: false
dominant: false
experience: experience/None
group u list: _253_
m user id: 0
owner id: 3313
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This BioBrick is the Gal4 recognition site. It can be used for the construction of promoters which should be regulated by activators or repressors containing a Gal4 DNA binding domain ( BBa_K105007).
mutable notes: This BioBrick contains the consensus sequence plus 5 spacer nucleotides on each side. The spacer is directly followed by the restriction site without a base in between. So when you construct multiple operator sites from this basic part you will have 16 bp between two consensus sequences.
mutable provenance: from oligos
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus sequence
sequence: https://synbiohub.org/public/igem/BBa_K105024_sequence/1

consensus sequence
type: SequenceAnnotation
persistent identity: annotation1989136
display id: annotation1989136
version: 1
top level: BBa_K105024
role: feature/conserved
role: SO:0000330
location: https://synbiohub.org/public/igem/BBa_K105024/annotation1989136/range1989136/1

https://synbiohub.org/public/igem/BBa_K105024/annotation1989136/range1989136/1
type: Range
persistent identity: range1989136
display id: range1989136
version: 1
direction: direction/forward
top level: BBa_K105024
start: 6
end: 22
orientation: inline

BBa_I746395
type: ComponentDefinition
persistent identity: BBa I746395
display id: BBa_I746395
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phiR73 delta inducing PLL promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the phiR73 delta activator on the PLL promoter. Note that the I746352 does contain a RBS.
mutable notes: I746315 was put upstream of I746325
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746325
component: BBa_I746315
sequence annotation: BBa_I746315
sequence annotation: BBa_I746325
sequence: https://synbiohub.org/public/igem/BBa_I746395_sequence/1

BBa_I746325
type: Component
persistent identity: component2377107
display id: component2377107
version: 1
top level: BBa_I746395
access: public
definition: https://synbiohub.org/public/igem/BBa_I746325/1

BBa_I746315
type: Component
persistent identity: component2377094
display id: component2377094
version: 1
top level: BBa_I746395
access: public
definition: https://synbiohub.org/public/igem/BBa_I746315/1

BBa_I746315
type: SequenceAnnotation
persistent identity: annotation2377094
display id: annotation2377094
version: 1
top level: BBa_I746395
location: https://synbiohub.org/public/igem/BBa_I746395/annotation2377094/range2377094/1
component: BBa_I746315

https://synbiohub.org/public/igem/BBa_I746395/annotation2377094/range2377094/1
type: Range
persistent identity: range2377094
display id: range2377094
version: 1
direction: direction/reverse
top level: BBa_I746395
start: 1
end: 2351
orientation: inline

BBa_I746325
type: SequenceAnnotation
persistent identity: annotation2377107
display id: annotation2377107
version: 1
top level: BBa_I746395
location: https://synbiohub.org/public/igem/BBa_I746395/annotation2377107/range2377107/1
component: BBa_I746325

https://synbiohub.org/public/igem/BBa_I746395/annotation2377107/range2377107/1
type: Range
persistent identity: range2377107
display id: range2377107
version: 1
direction: direction/reverse
top level: BBa_I746395
start: 2360
end: 3334
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0161
display id: BBa_C0161
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: autoinducer synthetase for AHL (no LVA)
creator: jcbraff
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
discontinued: false
dominant: true
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: same as C0061 except no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LuxI
sequence: https://synbiohub.org/public/igem/BBa_C0161_sequence/1

LuxI
type: SequenceAnnotation
persistent identity: annotation1891615
display id: annotation1891615
version: 1
top level: luxI
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C0161/annotation1891615/range1891615/1

https://synbiohub.org/public/igem/BBa_C0161/annotation1891615/range1891615/1
type: Range
persistent identity: range1891615
display id: range1891615
version: 1
direction: direction/forward
top level: luxI
start: 1
end: 585
orientation: inline

BBa_K118016
type: ComponentDefinition
persistent identity: BBa K118016
display id: BBa_K118016
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: glgC16 (glgC with G336D substitution)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' (ADP-glucose pyrophosphorylase) from ''Escherichia coli'' JM109 with the substitution G336D. This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung ''et al'', 1986; Meyer ''et al.'', 1988)
mutable notes: The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) by MABEL.
mutable provenance: ''Escherichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EcoRI site removed
sequence annotation: G336D
sequence: https://synbiohub.org/public/igem/BBa_K118016_sequence/1

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978558
display id: annotation1978558
version: 1
top level: BBa_K118016
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978558/range1978558/1

https://synbiohub.org/public/igem/BBa_K118016/annotation1978558/range1978558/1
type: Range
persistent identity: range1978558
display id: range1978558
version: 1
direction: direction/forward
top level: BBa_K118016
start: 571
end: 576
orientation: inline

G336D
type: SequenceAnnotation
persistent identity: annotation1978557
display id: annotation1978557
version: 1
top level: BBa_K118016
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978557/range1978557/1

https://synbiohub.org/public/igem/BBa_K118016/annotation1978557/range1978557/1
type: Range
persistent identity: range1978557
display id: range1978557
version: 1
direction: direction/forward
top level: BBa_K118016
start: 1006
end: 1008
orientation: inline

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978559
display id: annotation1978559
version: 1
top level: BBa_K118016
role: SO:1000008
role: feature/s mutation
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978559/range1978559/1

https://synbiohub.org/public/igem/BBa_K118016/annotation1978559/range1978559/1
type: Range
persistent identity: range1978559
display id: range1978559
version: 1
direction: direction/forward
top level: BBa_K118016
start: 1068
end: 1073
orientation: inline

BBa_P0453
type: ComponentDefinition
persistent identity: BBa P0453
display id: BBa_P0453
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> cII (p22) [S0155]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:11Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0034
component: BBa_B0012
component: BBa_C0053
sequence annotation: BBa_C0053
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_P0453_sequence/1

BBa_B0010
type: Component
persistent identity: component945042
display id: component945042
version: 1
top level: BBa_P0453
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component945022
display id: component945022
version: 1
top level: BBa_P0453
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0012
type: Component
persistent identity: component945052
display id: component945052
version: 1
top level: BBa_P0453
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0053
type: Component
persistent identity: component945034
display id: component945034
version: 1
top level: BBa_P0453
access: public
definition: https://synbiohub.org/public/igem/BBa_C0053/1

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation945034
display id: annotation945034
version: 1
top level: BBa_P0453
location: https://synbiohub.org/public/igem/BBa_P0453/annotation945034/range945034/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_P0453/annotation945034/range945034/1
type: Range
persistent identity: range945034
display id: range945034
version: 1
direction: direction/forward
top level: BBa_P0453
start: 19
end: 705
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation945022
display id: annotation945022
version: 1
top level: BBa_P0453
location: https://synbiohub.org/public/igem/BBa_P0453/annotation945022/range945022/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0453/annotation945022/range945022/1
type: Range
persistent identity: range945022
display id: range945022
version: 1
direction: direction/forward
top level: BBa_P0453
start: 1
end: 12
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation945052
display id: annotation945052
version: 1
top level: BBa_P0453
location: https://synbiohub.org/public/igem/BBa_P0453/annotation945052/range945052/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0453/annotation945052/range945052/1
type: Range
persistent identity: range945052
display id: range945052
version: 1
direction: direction/forward
top level: BBa_P0453
start: 827
end: 867
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation945042
display id: annotation945042
version: 1
top level: BBa_P0453
location: https://synbiohub.org/public/igem/BBa_P0453/annotation945042/range945042/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0453/annotation945042/range945042/1
type: Range
persistent identity: range945042
display id: range945042
version: 1
direction: direction/forward
top level: BBa_P0453
start: 739
end: 818
orientation: inline

BBa_K081005
type: ComponentDefinition
persistent identity: BBa K081005
display id: BBa_K081005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: constitutive promoter family member and RBS
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:34Z
discontinued: false
dominant: true
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Constitutive promoter (strong) with RBS (strong, efficiency=0.3)
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: Promoter: John Anderson. RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_J23100
sequence annotation: BBa_B0030
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K081005_sequence/1

BBa_B0030
type: Component
persistent identity: component1981865
display id: component1981865
version: 1
top level: BBa_K081005
access: public
definition: BBa_B0030

BBa_J23100
type: Component
persistent identity: component1981863
display id: component1981863
version: 1
top level: BBa_K081005
access: public
definition: https://synbiohub.org/public/igem/BBa_J23100/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1981865
display id: annotation1981865
version: 1
top level: BBa_K081005
location: https://synbiohub.org/public/igem/BBa_K081005/annotation1981865/range1981865/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081005/annotation1981865/range1981865/1
type: Range
persistent identity: range1981865
display id: range1981865
version: 1
direction: direction/forward
top level: BBa_K081005
start: 44
end: 58
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation1981863
display id: annotation1981863
version: 1
top level: BBa_K081005
location: https://synbiohub.org/public/igem/BBa_K081005/annotation1981863/range1981863/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K081005/annotation1981863/range1981863/1
type: Range
persistent identity: range1981863
display id: range1981863
version: 1
direction: direction/reverse
top level: BBa_K081005
start: 1
end: 35
orientation: inline

BBa_K118027
type: ComponentDefinition
persistent identity: BBa K118027
display id: BBa_K118027
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcstA + lacZ
creator: Chris French
created: 2008-10-22T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 837
owning group id: 163
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This part consists of the cstA promoter, which is repressed by high levels of glucose, coupled to the lacZ' reporter gene for promoter activity assays.
mutable notes: No special considerations.
mutable provenance: The cstA promoter and lacZ' are both from Escherichia coli.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_J15103
component: BBa_J15001
component: BBa_K118011
sequence annotation: BBa_J15001
sequence annotation: BBa_J15103
sequence annotation: BBa_K118011
sequence: https://synbiohub.org/public/igem/BBa_K118027_sequence/1

BBa_J15103
type: Component
persistent identity: component1985191
display id: component1985191
version: 1
top level: BBa_K118027
access: public
definition: https://synbiohub.org/public/igem/BBa_J15103/1

BBa_J15001
type: Component
persistent identity: component1985186
display id: component1985186
version: 1
top level: BBa_K118027
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118011
type: Component
persistent identity: component1985183
display id: component1985183
version: 1
top level: BBa_K118027
access: public
definition: BBa_K118011

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1985186
display id: annotation1985186
version: 1
top level: BBa_K118027
location: https://synbiohub.org/public/igem/BBa_K118027/annotation1985186/range1985186/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118027/annotation1985186/range1985186/1
type: Range
persistent identity: range1985186
display id: range1985186
version: 1
direction: direction/reverse
top level: BBa_K118027
start: 140
end: 149
orientation: inline

BBa_J15103
type: SequenceAnnotation
persistent identity: annotation1985191
display id: annotation1985191
version: 1
top level: BBa_K118027
location: https://synbiohub.org/public/igem/BBa_K118027/annotation1985191/range1985191/1
component: BBa_J15103

https://synbiohub.org/public/igem/BBa_K118027/annotation1985191/range1985191/1
type: Range
persistent identity: range1985191
display id: range1985191
version: 1
direction: direction/reverse
top level: BBa_K118027
start: 156
end: 386
orientation: inline

BBa_K118011
type: SequenceAnnotation
persistent identity: annotation1985183
display id: annotation1985183
version: 1
top level: BBa_K118027
location: https://synbiohub.org/public/igem/BBa_K118027/annotation1985183/range1985183/1
component: BBa_K118011

https://synbiohub.org/public/igem/BBa_K118027/annotation1985183/range1985183/1
type: Range
persistent identity: range1985183
display id: range1985183
version: 1
direction: direction/reverse
top level: BBa_K118027
start: 1
end: 131
orientation: inline

BBa_K081007
type: ComponentDefinition
persistent identity: BBa K081007
display id: BBa_K081007
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor (TERM-)
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: cI protein generator without terminator (strong RBS, efficiency=0.6)
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: cI: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0030
component: BBa_C0051
sequence annotation: BBa_C0051
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081007_sequence/1

BBa_B0030
type: Component
persistent identity: component2243981
display id: component2243981
version: 1
top level: BBa_K081007
access: public
definition: BBa_B0030

BBa_C0051
type: Component
persistent identity: component2243986
display id: component2243986
version: 1
top level: BBa_K081007
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2243986
display id: annotation2243986
version: 1
top level: BBa_K081007
location: https://synbiohub.org/public/igem/BBa_K081007/annotation2243986/range2243986/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K081007/annotation2243986/range2243986/1
type: Range
persistent identity: range2243986
display id: range2243986
version: 1
direction: direction/reverse
top level: BBa_K081007
start: 22
end: 796
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2243981
display id: annotation2243981
version: 1
top level: BBa_K081007
location: https://synbiohub.org/public/igem/BBa_K081007/annotation2243981/range2243981/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081007/annotation2243981/range2243981/1
type: Range
persistent identity: range2243981
display id: range2243981
version: 1
direction: direction/forward
top level: BBa_K081007
start: 1
end: 15
orientation: inline

BBa_J52028
type: ComponentDefinition
persistent identity: BBa J52028
display id: BBa_J52028
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: green fluorescent protein derived from A. victoria GFP (SwissProt: P42212) with PEST191 tag
creator: Jelka Pohar
created: 2006-10-14T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 855
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: GFP-linker-PEST191 is a part composed of GFP (as a reporter) linked with PEST191 through six aminoacid long protein linker. PEST191 is a sequence that leads towards degradation of protein linked with this sequence. It's estimated half-time is 2h.
mutable notes: GFP-linker-PEST191 is made by PCR overlap extension technique.
mutable provenance: GFP- PEST191-
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: start codon
sequence annotation: PEST191 (degradation flag)
sequence annotation: stop codon
sequence annotation: GFP-linker-PEST191
sequence annotation: GFP
sequence annotation: linker
sequence: https://synbiohub.org/public/igem/BBa_J52028_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1908104
display id: annotation1908104
version: 1
top level: BBa_J52028
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1908104/range1908104/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1908104/range1908104/1
type: Range
persistent identity: range1908104
display id: range1908104
version: 1
direction: direction/forward
top level: BBa_J52028
start: 1
end: 3
orientation: inline

PEST191 (degradation flag)
type: SequenceAnnotation
persistent identity: annotation1903016
display id: annotation1903016
version: 1
top level: BBa_J52028
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1903016/range1903016/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1903016/range1903016/1
type: Range
persistent identity: range1903016
display id: range1903016
version: 1
direction: direction/forward
top level: BBa_J52028
start: 736
end: 1221
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1908105
display id: annotation1908105
version: 1
top level: BBa_J52028
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1908105/range1908105/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1908105/range1908105/1
type: Range
persistent identity: range1908105
display id: range1908105
version: 1
direction: direction/forward
top level: BBa_J52028
start: 1219
end: 1221
orientation: inline

GFP-linker-PEST191
type: SequenceAnnotation
persistent identity: annotation1903017
display id: annotation1903017
version: 1
top level: BBa_J52028
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1903017/range1903017/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1903017/range1903017/1
type: Range
persistent identity: range1903017
display id: range1903017
version: 1
direction: direction/forward
top level: BBa_J52028
start: 1
end: 1221
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation1903014
display id: annotation1903014
version: 1
top level: BBa_J52028
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1903014/range1903014/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1903014/range1903014/1
type: Range
persistent identity: range1903014
display id: range1903014
version: 1
direction: direction/forward
top level: BBa_J52028
start: 1
end: 717
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation1903015
display id: annotation1903015
version: 1
top level: BBa_J52028
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_J52028/annotation1903015/range1903015/1

https://synbiohub.org/public/igem/BBa_J52028/annotation1903015/range1903015/1
type: Range
persistent identity: range1903015
display id: range1903015
version: 1
direction: direction/forward
top level: BBa_J52028
start: 718
end: 735
orientation: inline

BBa_K081013
type: ComponentDefinition
persistent identity: BBa K081013
display id: BBa_K081013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS - cI - RBS
creator: Lorenzo Pasotti, Paolo Magni
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Strong RBS (efficiency=0.6) - cI repressor - Strong RBS (efficiency=0.6).
mutable notes: We used BioBrick Standard Assembly.
mutable provenance: RBS: cI:
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_C0051
sequence annotation: BBa_C0051
sequence annotation: BBa_B0030
sequence: https://synbiohub.org/public/igem/BBa_K081013_sequence/1

BBa_B0030
type: Component
persistent identity: component2246143
display id: component2246143
version: 1
top level: BBa_K081013
access: public
definition: BBa_B0030

BBa_B0030
type: Component
persistent identity: component2246136
display id: component2246136
version: 1
top level: BBa_K081013
access: public
definition: BBa_B0030

BBa_C0051
type: Component
persistent identity: component2246141
display id: component2246141
version: 1
top level: BBa_K081013
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2246141
display id: annotation2246141
version: 1
top level: BBa_K081013
location: https://synbiohub.org/public/igem/BBa_K081013/annotation2246141/range2246141/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K081013/annotation2246141/range2246141/1
type: Range
persistent identity: range2246141
display id: range2246141
version: 1
direction: direction/reverse
top level: BBa_K081013
start: 22
end: 796
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246143
display id: annotation2246143
version: 1
top level: BBa_K081013
location: https://synbiohub.org/public/igem/BBa_K081013/annotation2246143/range2246143/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081013/annotation2246143/range2246143/1
type: Range
persistent identity: range2246143
display id: range2246143
version: 1
direction: direction/forward
top level: BBa_K081013
start: 805
end: 819
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246136
display id: annotation2246136
version: 1
top level: BBa_K081013
location: https://synbiohub.org/public/igem/BBa_K081013/annotation2246136/range2246136/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081013/annotation2246136/range2246136/1
type: Range
persistent identity: range2246136
display id: range2246136
version: 1
direction: direction/forward
top level: BBa_K081013
start: 1
end: 15
orientation: inline

BBa_B1005
type: ComponentDefinition
persistent identity: BBa B1005
display id: BBa_B1005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, small, %T~=25%
creator: Haiyao Huang
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~=25% * 6bp stem, 4nt loop * Bidirectional, estimated reverse %T~<10%
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 3 residues. Designed to be a terminator with low efficiency.
mutable provenance: antiquity
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: B1005
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B1005_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1898425
display id: annotation1898425
version: 1
top level: BBa_B1005
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898425/range1898425/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898425/range1898425/1
type: Range
persistent identity: range1898425
display id: range1898425
version: 1
direction: direction/forward
top level: BBa_B1005
start: 26
end: 28
orientation: inline

B1005
type: SequenceAnnotation
persistent identity: annotation1898424
display id: annotation1898424
version: 1
top level: BBa_B1005
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898424/range1898424/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898424/range1898424/1
type: Range
persistent identity: range1898424
display id: range1898424
version: 1
direction: direction/forward
top level: BBa_B1005
start: 1
end: 34
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898427
display id: annotation1898427
version: 1
top level: BBa_B1005
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898427/range1898427/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898427/range1898427/1
type: Range
persistent identity: range1898427
display id: range1898427
version: 1
direction: direction/reverse
top level: BBa_B1005
start: 7
end: 9
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1898426
display id: annotation1898426
version: 1
top level: BBa_B1005
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1005/annotation1898426/range1898426/1

https://synbiohub.org/public/igem/BBa_B1005/annotation1898426/range1898426/1
type: Range
persistent identity: range1898426
display id: range1898426
version: 1
direction: direction/forward
top level: BBa_B1005
start: 10
end: 25
orientation: inline

BBa_I1466
type: ComponentDefinition
persistent identity: BBa I1466
display id: BBa_I1466
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RhlR protein generator (LVA-)
creator: Barry Canton
created: 2004-08-09T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Elowitz RBS. RhlR CDS (no LVA tag) and terminator. Same as I0466 but no LVA tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0171
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0171
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I1466_sequence/1

BBa_B0034
type: Component
persistent identity: component1040123
display id: component1040123
version: 1
top level: BBa_I1466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1040130
display id: component1040130
version: 1
top level: BBa_I1466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_C0171
type: Component
persistent identity: component1040125
display id: component1040125
version: 1
top level: BBa_I1466
access: public
definition: rhIR

BBa_B0012
type: Component
persistent identity: component1040140
display id: component1040140
version: 1
top level: BBa_I1466
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1040140
display id: annotation1040140
version: 1
top level: BBa_I1466
location: https://synbiohub.org/public/igem/BBa_I1466/annotation1040140/range1040140/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I1466/annotation1040140/range1040140/1
type: Range
persistent identity: range1040140
display id: range1040140
version: 1
direction: direction/forward
top level: BBa_I1466
start: 844
end: 884
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1040123
display id: annotation1040123
version: 1
top level: BBa_I1466
location: https://synbiohub.org/public/igem/BBa_I1466/annotation1040123/range1040123/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I1466/annotation1040123/range1040123/1
type: Range
persistent identity: range1040123
display id: range1040123
version: 1
direction: direction/forward
top level: BBa_I1466
start: 1
end: 12
orientation: inline

BBa_C0171
type: SequenceAnnotation
persistent identity: annotation1040125
display id: annotation1040125
version: 1
top level: BBa_I1466
location: https://synbiohub.org/public/igem/BBa_I1466/annotation1040125/range1040125/1
component: BBa_C0171

https://synbiohub.org/public/igem/BBa_I1466/annotation1040125/range1040125/1
type: Range
persistent identity: range1040125
display id: range1040125
version: 1
direction: direction/forward
top level: BBa_I1466
start: 19
end: 747
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1040130
display id: annotation1040130
version: 1
top level: BBa_I1466
location: https://synbiohub.org/public/igem/BBa_I1466/annotation1040130/range1040130/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I1466/annotation1040130/range1040130/1
type: Range
persistent identity: range1040130
display id: range1040130
version: 1
direction: direction/forward
top level: BBa_I1466
start: 756
end: 835
orientation: inline

BBa_I13513
type: ComponentDefinition
persistent identity: BBa I13513
display id: BBa_I13513
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening Plasmid 2
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: The second screening plasmid. Same as the first, except with stronger RBSs. Ends should be capped with I13450.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I0500
component: BBa_B0012
component: BBa_E0040
component: BBa_E1010
component: BBa_B0010
component: BBa_B0034
component: BBa_I13452
sequence annotation: BBa_E1010
sequence annotation: BBa_E0040
sequence annotation: BBa_I0500
sequence annotation: BBa_I13452
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_I13513_sequence/1

BBa_I0500
type: Component
persistent identity: component1505312
display id: component1505312
version: 1
top level: BBa_I13513
access: public
definition: https://synbiohub.org/public/igem/BBa_I0500/1

BBa_B0012
type: Component
persistent identity: component1505361
display id: component1505361
version: 1
top level: BBa_I13513
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1505346
display id: component1505346
version: 1
top level: BBa_I13513
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_E1010
type: Component
persistent identity: component1505324
display id: component1505324
version: 1
top level: BBa_I13513
access: public
definition: mRFP1

BBa_B0010
type: Component
persistent identity: component1505351
display id: component1505351
version: 1
top level: BBa_I13513
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1505344
display id: component1505344
version: 1
top level: BBa_I13513
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I13452
type: Component
persistent identity: component1505339
display id: component1505339
version: 1
top level: BBa_I13513
access: public
definition: BBa_I13452

BBa_B0034
type: Component
persistent identity: component1505317
display id: component1505317
version: 1
top level: BBa_I13513
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505324
display id: annotation1505324
version: 1
top level: BBa_I13513
location: https://synbiohub.org/public/igem/BBa_I13513/annotation1505324/range1505324/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13513/annotation1505324/range1505324/1
type: Range
persistent identity: range1505324
display id: range1505324
version: 1
direction: direction/forward
top level: BBa_I13513
start: 1237
end: 1917
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1505346
display id: annotation1505346
version: 1
top level: BBa_I13513
location: https://synbiohub.org/public/igem/BBa_I13513/annotation1505346/range1505346/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13513/annotation1505346/range1505346/1
type: Range
persistent identity: range1505346
display id: range1505346
version: 1
direction: direction/forward
top level: BBa_I13513
start: 2029
end: 2748
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1505312
display id: annotation1505312
version: 1
top level: BBa_I13513
location: https://synbiohub.org/public/igem/BBa_I13513/annotation1505312/range1505312/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13513/annotation1505312/range1505312/1
type: Range
persistent identity: range1505312
display id: range1505312
version: 1
direction: direction/forward
top level: BBa_I13513
start: 1
end: 1210
orientation: inline

BBa_I13452
type: SequenceAnnotation
persistent identity: annotation1505339
display id: annotation1505339
version: 1
top level: BBa_I13513
location: https://synbiohub.org/public/igem/BBa_I13513/annotation1505339/range1505339/1
component: BBa_I13452

https://synbiohub.org/public/igem/BBa_I13513/annotation1505339/range1505339/1
type: Range
persistent identity: range1505339
display id: range1505339
version: 1
direction: direction/forward
top level: BBa_I13513
start: 1951
end: 2002
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505344
display id: annotation1505344
version: 1
top level: BBa_I13513
location: https://synbiohub.org/public/igem/BBa_I13513/annotation1505344/range1505344/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13513/annotation1505344/range1505344/1
type: Range
persistent identity: range1505344
display id: range1505344
version: 1
direction: direction/forward
top level: BBa_I13513
start: 2011
end: 2022
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505317
display id: annotation1505317
version: 1
top level: BBa_I13513
location: https://synbiohub.org/public/igem/BBa_I13513/annotation1505317/range1505317/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13513/annotation1505317/range1505317/1
type: Range
persistent identity: range1505317
display id: range1505317
version: 1
direction: direction/forward
top level: BBa_I13513
start: 1219
end: 1230
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1505361
display id: annotation1505361
version: 1
top level: BBa_I13513
location: https://synbiohub.org/public/igem/BBa_I13513/annotation1505361/range1505361/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13513/annotation1505361/range1505361/1
type: Range
persistent identity: range1505361
display id: range1505361
version: 1
direction: direction/forward
top level: BBa_I13513
start: 2845
end: 2885
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1505351
display id: annotation1505351
version: 1
top level: BBa_I13513
location: https://synbiohub.org/public/igem/BBa_I13513/annotation1505351/range1505351/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13513/annotation1505351/range1505351/1
type: Range
persistent identity: range1505351
display id: range1505351
version: 1
direction: direction/forward
top level: BBa_I13513
start: 2757
end: 2836
orientation: inline

BBa_B0021
type: ComponentDefinition
persistent identity: BBa B0021
display id: BBa_B0021
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: LuxICDABEG (+/-), reversed
creator: Caitlin Conboy
created: 2003-10-13T11:00:00Z
modified: 2015-08-31T04:07:20Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Bidirectional transcriptional terminator cloned in the reverse direction of B0011. 22 bp stem-loop
mutable notes: Cloned with primer-dimers into pSB1A2
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0021
sequence: https://synbiohub.org/public/igem/BBa_B0021_sequence/1

BBa_B0021
type: SequenceAnnotation
persistent identity: annotation7024
display id: annotation7024
version: 1
top level: BBa_B0021
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B0021/annotation7024/range7024/1

https://synbiohub.org/public/igem/BBa_B0021/annotation7024/range7024/1
type: Range
persistent identity: range7024
display id: range7024
version: 1
direction: direction/forward
top level: BBa_B0021
start: 1
end: 46
orientation: inline

BBa_I746394
type: ComponentDefinition
persistent identity: BBa I746394
display id: BBa_I746394
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: phiR73 delta inducing Psid promoter
creator: Stefan Milde
created: 2007-09-25T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This construct is used to screen the level of induction of the phiR73 delta activator on the Psid promoter. Note that the I746352 does contain a RBS.
mutable notes: I746315 was put upstream of I746324
mutable provenance: own construct
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I746315
component: BBa_I746324
sequence annotation: BBa_I746324
sequence annotation: BBa_I746315
sequence: https://synbiohub.org/public/igem/BBa_I746394_sequence/1

BBa_I746315
type: Component
persistent identity: component2292132
display id: component2292132
version: 1
top level: BBa_I746394
access: public
definition: https://synbiohub.org/public/igem/BBa_I746315/1

BBa_I746324
type: Component
persistent identity: component2292145
display id: component2292145
version: 1
top level: BBa_I746394
access: public
definition: BBa_I746324

BBa_I746324
type: SequenceAnnotation
persistent identity: annotation2292145
display id: annotation2292145
version: 1
top level: BBa_I746394
location: https://synbiohub.org/public/igem/BBa_I746394/annotation2292145/range2292145/1
component: BBa_I746324

https://synbiohub.org/public/igem/BBa_I746394/annotation2292145/range2292145/1
type: Range
persistent identity: range2292145
display id: range2292145
version: 1
direction: direction/reverse
top level: BBa_I746394
start: 2360
end: 3335
orientation: inline

BBa_I746315
type: SequenceAnnotation
persistent identity: annotation2292132
display id: annotation2292132
version: 1
top level: BBa_I746394
location: https://synbiohub.org/public/igem/BBa_I746394/annotation2292132/range2292132/1
component: BBa_I746315

https://synbiohub.org/public/igem/BBa_I746394/annotation2292132/range2292132/1
type: Range
persistent identity: range2292132
display id: range2292132
version: 1
direction: direction/reverse
top level: BBa_I746394
start: 1
end: 2351
orientation: inline

BBa_I741005
type: ComponentDefinition
persistent identity: BBa I741005
display id: BBa_I741005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: XylR Transcriptional Regulator Right Facing
creator: Galen Lynch
created: 2007-06-06T11:00:00Z
modified: 2015-08-31T04:08:01Z
discontinued: false
dominant: false
experience: experience/Issues
group u list: _143_
m user id: 0
owner id: 1939
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Code for XylR Transcriptional regulator of xylose catbolization pathway. XylR recognizes xylulose (the isomer of xylose) and promotes the transcription for the high affinity transporter (XylF,G,H) and catbolization enzymes XylA,B.
mutable notes: Open reading frame
mutable provenance: E. coli Chromosome
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: stop codon
sequence annotation: xylR
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_I741005_sequence/1

stop codon
type: SequenceAnnotation
persistent identity: annotation1935095
display id: annotation1935095
version: 1
top level: BBa_I741005
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_I741005/annotation1935095/range1935095/1

https://synbiohub.org/public/igem/BBa_I741005/annotation1935095/range1935095/1
type: Range
persistent identity: range1935095
display id: range1935095
version: 1
direction: direction/forward
top level: BBa_I741005
start: 1177
end: 1179
orientation: inline

xylR
type: SequenceAnnotation
persistent identity: annotation1935096
display id: annotation1935096
version: 1
top level: BBa_I741005
role: SO:0000316
role: feature/protein
location: https://synbiohub.org/public/igem/BBa_I741005/annotation1935096/range1935096/1

https://synbiohub.org/public/igem/BBa_I741005/annotation1935096/range1935096/1
type: Range
persistent identity: range1935096
display id: range1935096
version: 1
direction: direction/forward
top level: BBa_I741005
start: 4
end: 1176
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1935093
display id: annotation1935093
version: 1
top level: BBa_I741005
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I741005/annotation1935093/range1935093/1

https://synbiohub.org/public/igem/BBa_I741005/annotation1935093/range1935093/1
type: Range
persistent identity: range1935093
display id: range1935093
version: 1
direction: direction/forward
top level: BBa_I741005
start: 1
end: 3
orientation: inline

BBa_S0103
type: ComponentDefinition
persistent identity: BBa S0103
display id: BBa_S0103
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.C0051
creator: Caitlin Conboy and Jennifer Braff
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0051
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence: https://synbiohub.org/public/igem/BBa_S0103_sequence/1

BBa_C0051
type: Component
persistent identity: component939758
display id: component939758
version: 1
top level: BBa_S0103
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_B0034
type: Component
persistent identity: component939748
display id: component939748
version: 1
top level: BBa_S0103
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939748
display id: annotation939748
version: 1
top level: BBa_S0103
location: https://synbiohub.org/public/igem/BBa_S0103/annotation939748/range939748/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0103/annotation939748/range939748/1
type: Range
persistent identity: range939748
display id: range939748
version: 1
direction: direction/forward
top level: BBa_S0103
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation939758
display id: annotation939758
version: 1
top level: BBa_S0103
location: https://synbiohub.org/public/igem/BBa_S0103/annotation939758/range939758/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_S0103/annotation939758/range939758/1
type: Range
persistent identity: range939758
display id: range939758
version: 1
direction: direction/forward
top level: BBa_S0103
start: 19
end: 768
orientation: inline

BBa_S03889
type: ComponentDefinition
persistent identity: BBa S03889
display id: BBa_S03889
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0062:S03873
creator: Kathy Lam
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:32Z
discontinued: false
dominant: false
group u list: _82_
m user id: 0
owner id: 927
owning group id: 82
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_R0062
component: BBa_S03873
sequence annotation: BBa_S03873
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_S03889_sequence/1

BBa_R0062
type: Component
persistent identity: component2245817
display id: component2245817
version: 1
top level: BBa_S03889
access: public
definition: https://synbiohub.org/public/igem/BBa_R0062/1

BBa_S03873
type: Component
persistent identity: component2245827
display id: component2245827
version: 1
top level: BBa_S03889
access: public
definition: https://synbiohub.org/public/igem/BBa_S03873/1

BBa_S03873
type: SequenceAnnotation
persistent identity: annotation2245827
display id: annotation2245827
version: 1
top level: BBa_S03889
location: https://synbiohub.org/public/igem/BBa_S03889/annotation2245827/range2245827/1
component: BBa_S03873

https://synbiohub.org/public/igem/BBa_S03889/annotation2245827/range2245827/1
type: Range
persistent identity: range2245827
display id: range2245827
version: 1
direction: direction/reverse
top level: BBa_S03889
start: 64
end: 787
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2245817
display id: annotation2245817
version: 1
top level: BBa_S03889
location: https://synbiohub.org/public/igem/BBa_S03889/annotation2245817/range2245817/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_S03889/annotation2245817/range2245817/1
type: Range
persistent identity: range2245817
display id: range2245817
version: 1
direction: direction/forward
top level: BBa_S03889
start: 1
end: 55
orientation: inline

BBa_K118017
type: ComponentDefinition
persistent identity: BBa K118017
display id: BBa_K118017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: rbs+glgC (ADP-glucose pyrophosphorylase)
creator: Andrew Hall
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the coding sequence of ''glgC'' from ''Escherichia coli'' JM109 with added ribosome binding site (rbs). It encodes ADP-glucose pyrophosphorylase, which catalyses Glucose 1-phosphate + ATP -> ADP-glucose + PPi, a step in the production of glycogen from glucose.
mutable notes: No special considerations.
mutable provenance: Escherichia coli JM109 genomic DNA.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_K118015
sequence annotation: BBa_J15001
sequence annotation: BBa_K118015
sequence: https://synbiohub.org/public/igem/BBa_K118017_sequence/1

BBa_J15001
type: Component
persistent identity: component1978627
display id: component1978627
version: 1
top level: BBa_K118017
access: public
definition: https://synbiohub.org/public/igem/BBa_J15001/1

BBa_K118015
type: Component
persistent identity: component1978630
display id: component1978630
version: 1
top level: BBa_K118017
access: public
definition: BBa_K118015

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation1978627
display id: annotation1978627
version: 1
top level: BBa_K118017
location: https://synbiohub.org/public/igem/BBa_K118017/annotation1978627/range1978627/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K118017/annotation1978627/range1978627/1
type: Range
persistent identity: range1978627
display id: range1978627
version: 1
direction: direction/reverse
top level: BBa_K118017
start: 1
end: 10
orientation: inline

BBa_K118015
type: SequenceAnnotation
persistent identity: annotation1978630
display id: annotation1978630
version: 1
top level: BBa_K118017
location: https://synbiohub.org/public/igem/BBa_K118017/annotation1978630/range1978630/1
component: BBa_K118015

https://synbiohub.org/public/igem/BBa_K118017/annotation1978630/range1978630/1
type: Range
persistent identity: range1978630
display id: range1978630
version: 1
direction: direction/reverse
top level: BBa_K118017
start: 17
end: 1315
orientation: inline

BBa_I732812
type: ComponentDefinition
persistent identity: BBa I732812
display id: BBa_I732812
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ARL2A0301 (RBS+, TERM-)
creator: Zhan Jian
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:07:59Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: N/A
mutable notes: N/A
mutable provenance: N/A
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_I732112
component: BBa_B0034
sequence annotation: BBa_I732112
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I732812_sequence/1

BBa_I732112
type: Component
persistent identity: component1946199
display id: component1946199
version: 1
top level: BBa_I732812
access: public
definition: ARL2A0301

BBa_B0034
type: Component
persistent identity: component1946198
display id: component1946198
version: 1
top level: BBa_I732812
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_I732112
type: SequenceAnnotation
persistent identity: annotation1946199
display id: annotation1946199
version: 1
top level: BBa_I732812
location: https://synbiohub.org/public/igem/BBa_I732812/annotation1946199/range1946199/1
component: BBa_I732112

https://synbiohub.org/public/igem/BBa_I732812/annotation1946199/range1946199/1
type: Range
persistent identity: range1946199
display id: range1946199
version: 1
direction: direction/reverse
top level: BBa_I732812
start: 19
end: 1104
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1946198
display id: annotation1946198
version: 1
top level: BBa_I732812
location: https://synbiohub.org/public/igem/BBa_I732812/annotation1946198/range1946198/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I732812/annotation1946198/range1946198/1
type: Range
persistent identity: range1946198
display id: range1946198
version: 1
direction: direction/reverse
top level: BBa_I732812
start: 1
end: 12
orientation: inline

BBa_I750013
type: ComponentDefinition
persistent identity: BBa I750013
display id: BBa_I750013
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: ComAalt
creator: Patricia Illing
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:08:05Z
discontinued: false
dominant: false
group u list: _132_
m user id: 0
owner id: 1683
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This protein is part of a two part signaling system. Phosphorylation of ComA by ComP(a cell surface receptor) results in its activation. It then initiates transcription from a specific response promoter. This protein is not endogenous to E. coli.
mutable provenance: Synthesized based on genomic sequence in B. subtilus
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I750013_sequence/1

BBa_P0412
type: ComponentDefinition
persistent identity: BBa P0412
display id: BBa_P0412
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PoPS -> LacI [S0150]
creator: Randy Rettberg
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0010
component: BBa_B0012
component: BBa_C0012
component: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_P0412_sequence/1

BBa_B0010
type: Component
persistent identity: component944844
display id: component944844
version: 1
top level: BBa_P0412
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component944854
display id: component944854
version: 1
top level: BBa_P0412
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_C0012
type: Component
persistent identity: component944837
display id: component944837
version: 1
top level: BBa_P0412
access: public
definition: https://synbiohub.org/public/igem/BBa_C0012/1

BBa_B0034
type: Component
persistent identity: component944827
display id: component944827
version: 1
top level: BBa_P0412
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation944837
display id: annotation944837
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944837/range944837/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_P0412/annotation944837/range944837/1
type: Range
persistent identity: range944837
display id: range944837
version: 1
direction: direction/forward
top level: BBa_P0412
start: 19
end: 1146
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944854
display id: annotation944854
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944854/range944854/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0412/annotation944854/range944854/1
type: Range
persistent identity: range944854
display id: range944854
version: 1
direction: direction/forward
top level: BBa_P0412
start: 1268
end: 1308
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944827
display id: annotation944827
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944827/range944827/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0412/annotation944827/range944827/1
type: Range
persistent identity: range944827
display id: range944827
version: 1
direction: direction/forward
top level: BBa_P0412
start: 1
end: 12
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944844
display id: annotation944844
version: 1
top level: BBa_P0412
location: https://synbiohub.org/public/igem/BBa_P0412/annotation944844/range944844/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0412/annotation944844/range944844/1
type: Range
persistent identity: range944844
display id: range944844
version: 1
direction: direction/forward
top level: BBa_P0412
start: 1180
end: 1259
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06505
display id: BBa_J06505
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: monomeric RFP optimized for bacteria
creator: ytwang
created: 2005-07-17T11:00:00Z
modified: 2015-08-31T04:08:18Z
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 0
owner id: 340
owning group id: 20
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends with the LVA tag using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible and with an LVA tag added.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: LVA
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06505_sequence/1

LVA
type: SequenceAnnotation
persistent identity: annotation1585887
display id: annotation1585887
version: 1
top level: mCherry
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1

https://synbiohub.org/public/igem/BBa_J06505/annotation1585887/range1585887/1
type: Range
persistent identity: range1585887
display id: range1585887
version: 1
direction: direction/forward
top level: mCherry
start: 709
end: 717
orientation: inline

mCherry
type: SequenceAnnotation
persistent identity: annotation1585879
display id: annotation1585879
version: 1
top level: mCherry
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1

https://synbiohub.org/public/igem/BBa_J06505/annotation1585879/range1585879/1
type: Range
persistent identity: range1585879
display id: range1585879
version: 1
direction: direction/forward
top level: mCherry
start: 1
end: 708
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585880
display id: annotation1585880
version: 1
top level: mCherry
role: SO:0001059
role: feature/mutation
location: https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1

https://synbiohub.org/public/igem/BBa_J06505/annotation1585880/range1585880/1
type: Range
persistent identity: range1585880
display id: range1585880
version: 1
direction: direction/forward
top level: mCherry
start: 352
end: 352
orientation: inline

BBa_I13450
type: ComponentDefinition
persistent identity: BBa I13450
display id: BBa_I13450
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Removes BioBrick End
creator: jkm
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Contains both an early MfeI site and a late NsiI site. Cutting with Mfe and Spe and ligation to a BB part cut EX will form two mixed sites, neither of which can be recut. Cutting with Xba and Nsi and ligation to a BB part cut SP will again form two mixed sites, neither of which can be recut.
mutable notes: Should be grown in pSB1A2, as Nsi cuts both pSB2K3 and pSB1AK3 and Mfe cuts pSB4A3. 70 bp from Xba to Nsi or Mfe to Spe, the minimum for gel extraction. The middle is largely noise, to raise the size of the part.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NsiI
sequence annotation: MfeI
sequence: https://synbiohub.org/public/igem/BBa_I13450_sequence/1

NsiI
type: SequenceAnnotation
persistent identity: annotation1494630
display id: annotation1494630
version: 1
top level: BBa_I13450
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13450/annotation1494630/range1494630/1

https://synbiohub.org/public/igem/BBa_I13450/annotation1494630/range1494630/1
type: Range
persistent identity: range1494630
display id: range1494630
version: 1
direction: direction/forward
top level: BBa_I13450
start: 68
end: 73
orientation: inline

MfeI
type: SequenceAnnotation
persistent identity: annotation1494720
display id: annotation1494720
version: 1
top level: BBa_I13450
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I13450/annotation1494720/range1494720/1

https://synbiohub.org/public/igem/BBa_I13450/annotation1494720/range1494720/1
type: Range
persistent identity: range1494720
display id: range1494720
version: 1
direction: direction/forward
top level: BBa_I13450
start: 2
end: 7
orientation: inline

BBa_S01022
type: ComponentDefinition
persistent identity: BBa S01022
display id: BBa_S01022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B0034.E0020
creator: Randy Rettberg
created: 2005-06-15T11:00:00Z
modified: 2015-05-08T01:14:17Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 25
owning group id: 1
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Intermediate Part from Box 7
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E0020
component: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_S01022_sequence/1

BBa_E0020
type: Component
persistent identity: component1520219
display id: component1520219
version: 1
top level: BBa_S01022
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0034
type: Component
persistent identity: component1520217
display id: component1520217
version: 1
top level: BBa_S01022
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1520219
display id: annotation1520219
version: 1
top level: BBa_S01022
location: https://synbiohub.org/public/igem/BBa_S01022/annotation1520219/range1520219/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_S01022/annotation1520219/range1520219/1
type: Range
persistent identity: range1520219
display id: range1520219
version: 1
direction: direction/forward
top level: BBa_S01022
start: 19
end: 741
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1520217
display id: annotation1520217
version: 1
top level: BBa_S01022
location: https://synbiohub.org/public/igem/BBa_S01022/annotation1520217/range1520217/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S01022/annotation1520217/range1520217/1
type: Range
persistent identity: range1520217
display id: range1520217
version: 1
direction: direction/forward
top level: BBa_S01022
start: 1
end: 12
orientation: inline

BBa_E2030
type: ComponentDefinition
persistent identity: BBa E2030
display id: BBa_E2030
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: enhanced version of EYFP, yeast-optimized
creator: ryu
created: 2005-01-24T12:00:00Z
modified: 2015-08-31T04:07:26Z
discontinued: false
dominant: false
experience: experience/None
group u list: _8_
m user id: 0
owner id: 230
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Venus YFP, yeast codon optimized. EX515/EM528
mutable notes: Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2020 (Cerulean CFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Cerulean CFP (BBa_E2020) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).
mutable provenance: nagai T. et al. (2002) Nat Biot (20):87-90
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: MATSG linker
sequence annotation: Venus YFP
sequence annotation: GSGTA linker
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E2030_sequence/1

MATSG linker
type: SequenceAnnotation
persistent identity: annotation1431979
display id: annotation1431979
version: 1
top level: BBa_E2030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431979/range1431979/1

https://synbiohub.org/public/igem/BBa_E2030/annotation1431979/range1431979/1
type: Range
persistent identity: range1431979
display id: range1431979
version: 1
direction: direction/forward
top level: BBa_E2030
start: 1
end: 15
orientation: inline

Venus YFP
type: SequenceAnnotation
persistent identity: annotation1431978
display id: annotation1431978
version: 1
top level: BBa_E2030
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431978/range1431978/1

https://synbiohub.org/public/igem/BBa_E2030/annotation1431978/range1431978/1
type: Range
persistent identity: range1431978
display id: range1431978
version: 1
direction: direction/forward
top level: BBa_E2030
start: 1
end: 747
orientation: inline

GSGTA linker
type: SequenceAnnotation
persistent identity: annotation1431980
display id: annotation1431980
version: 1
top level: BBa_E2030
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_E2030/annotation1431980/range1431980/1

https://synbiohub.org/public/igem/BBa_E2030/annotation1431980/range1431980/1
type: Range
persistent identity: range1431980
display id: range1431980
version: 1
direction: direction/forward
top level: BBa_E2030
start: 733
end: 747
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214039
display id: annotation2214039
version: 1
top level: BBa_E2030
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_E2030/annotation2214039/range2214039/1

https://synbiohub.org/public/igem/BBa_E2030/annotation2214039/range2214039/1
type: Range
persistent identity: range2214039
display id: range2214039
version: 1
direction: direction/forward
top level: BBa_E2030
start: 754
end: 778
orientation: inline

pLac+cI
type: ComponentDefinition
persistent identity: BBa I763005
display id: BBa_I763005
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: cI repressor (+LVA) regulated by pLac Promoter
creator: Alice Pasini
created: 2007-07-29T11:00:00Z
modified: 2015-08-31T04:08:08Z
discontinued: false
dominant: true
experience: experience/Issues
group u list: _111_
m user id: 0
owner id: 2088
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Coding region for the cI repressor (C0051) based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051). The expression of the protein is under the regulation of pLac Promoter (R0010).
mutable notes: Intermediate
mutable provenance: Igem 2007 plates
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0051
component: BBa_J04500
sequence annotation: BBa_C0051
sequence annotation: BBa_J04500
sequence: https://synbiohub.org/public/igem/BBa_I763005_sequence/1

BBa_C0051
type: Component
persistent identity: component2245596
display id: component2245596
version: 1
top level: pLac+cI
access: public
definition: https://synbiohub.org/public/igem/BBa_C0051/1

BBa_J04500
type: Component
persistent identity: component2245592
display id: component2245592
version: 1
top level: pLac+cI
access: public
definition: BBa_J04500

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2245596
display id: annotation2245596
version: 1
top level: pLac+cI
location: https://synbiohub.org/public/igem/BBa_I763005/annotation2245596/range2245596/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I763005/annotation2245596/range2245596/1
type: Range
persistent identity: range2245596
display id: range2245596
version: 1
direction: direction/reverse
top level: pLac+cI
start: 227
end: 1001
orientation: inline

BBa_J04500
type: SequenceAnnotation
persistent identity: annotation2245592
display id: annotation2245592
version: 1
top level: pLac+cI
location: https://synbiohub.org/public/igem/BBa_I763005/annotation2245592/range2245592/1
component: BBa_J04500

https://synbiohub.org/public/igem/BBa_I763005/annotation2245592/range2245592/1
type: Range
persistent identity: range2245592
display id: range2245592
version: 1
direction: direction/reverse
top level: pLac+cI
start: 1
end: 220
orientation: inline

BBa_K137043
type: ComponentDefinition
persistent identity: BBa K137043
display id: BBa_K137043
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulatory SSM device: Promoter with (TA)9 repeat expressing GFP
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Promoter with (TA)9 repeat upstream of RBS + GFP + TT
mutable notes: Promoter with (TA)9 repeat upstream of RBS + GFP + TT
mutable provenance: K137030 + I13504
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0012
component: BBa_E0040
component: BBa_K137030
component: BBa_B0034
component: BBa_B0010
sequence annotation: BBa_K137030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_K137043_sequence/1

BBa_B0012
type: Component
persistent identity: component1967989
display id: component1967989
version: 1
top level: BBa_K137043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0040
type: Component
persistent identity: component1967986
display id: component1967986
version: 1
top level: BBa_K137043
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_K137030
type: Component
persistent identity: component1967982
display id: component1967982
version: 1
top level: BBa_K137043
access: public
definition: https://synbiohub.org/public/igem/BBa_K137030/1

BBa_B0034
type: Component
persistent identity: component1967984
display id: component1967984
version: 1
top level: BBa_K137043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0010
type: Component
persistent identity: component1967987
display id: component1967987
version: 1
top level: BBa_K137043
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_K137030
type: SequenceAnnotation
persistent identity: annotation1967982
display id: annotation1967982
version: 1
top level: BBa_K137043
location: https://synbiohub.org/public/igem/BBa_K137043/annotation1967982/range1967982/1
component: BBa_K137030

https://synbiohub.org/public/igem/BBa_K137043/annotation1967982/range1967982/1
type: Range
persistent identity: range1967982
display id: range1967982
version: 1
direction: direction/reverse
top level: BBa_K137043
start: 1
end: 37
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1967986
display id: annotation1967986
version: 1
top level: BBa_K137043
location: https://synbiohub.org/public/igem/BBa_K137043/annotation1967986/range1967986/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K137043/annotation1967986/range1967986/1
type: Range
persistent identity: range1967986
display id: range1967986
version: 1
direction: direction/reverse
top level: BBa_K137043
start: 64
end: 783
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1967984
display id: annotation1967984
version: 1
top level: BBa_K137043
location: https://synbiohub.org/public/igem/BBa_K137043/annotation1967984/range1967984/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137043/annotation1967984/range1967984/1
type: Range
persistent identity: range1967984
display id: range1967984
version: 1
direction: direction/reverse
top level: BBa_K137043
start: 46
end: 57
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1967989
display id: annotation1967989
version: 1
top level: BBa_K137043
location: https://synbiohub.org/public/igem/BBa_K137043/annotation1967989/range1967989/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137043/annotation1967989/range1967989/1
type: Range
persistent identity: range1967989
display id: range1967989
version: 1
direction: direction/forward
top level: BBa_K137043
start: 880
end: 920
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1967987
display id: annotation1967987
version: 1
top level: BBa_K137043
location: https://synbiohub.org/public/igem/BBa_K137043/annotation1967987/range1967987/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137043/annotation1967987/range1967987/1
type: Range
persistent identity: range1967987
display id: range1967987
version: 1
direction: direction/forward
top level: BBa_K137043
start: 792
end: 871
orientation: inline

BBa_B1008
type: ComponentDefinition
persistent identity: BBa B1008
display id: BBa_B1008
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Terminator (artificial, large, %T~=70)
creator: Haiyao Huang
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
discontinued: false
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Artificial terminator, estimated %T~=70% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~=80%
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 5 residues.
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pair
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence annotation: B1008
sequence: https://synbiohub.org/public/igem/BBa_B1008_sequence/1

PolyA
type: SequenceAnnotation
persistent identity: annotation1901008
display id: annotation1901008
version: 1
top level: BBa_B1008
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901008/range1901008/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901008/range1901008/1
type: Range
persistent identity: range1901008
display id: range1901008
version: 1
direction: direction/forward
top level: BBa_B1008
start: 32
end: 36
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1901006
display id: annotation1901006
version: 1
top level: BBa_B1008
role: feature/stem loop
role: SO:0000313
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901006/range1901006/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901006/range1901006/1
type: Range
persistent identity: range1901006
display id: range1901006
version: 1
direction: direction/forward
top level: BBa_B1008
start: 10
end: 31
orientation: inline

B1008
type: SequenceAnnotation
persistent identity: annotation1901005
display id: annotation1901005
version: 1
top level: BBa_B1008
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901005/range1901005/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901005/range1901005/1
type: Range
persistent identity: range1901005
display id: range1901005
version: 1
direction: direction/forward
top level: BBa_B1008
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901007
display id: annotation1901007
version: 1
top level: BBa_B1008
role: feature/polya
role: SO:0000553
location: https://synbiohub.org/public/igem/BBa_B1008/annotation1901007/range1901007/1

https://synbiohub.org/public/igem/BBa_B1008/annotation1901007/range1901007/1
type: Range
persistent identity: range1901007
display id: range1901007
version: 1
direction: direction/reverse
top level: BBa_B1008
start: 5
end: 9
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2002
display id: BBa_R2002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter, Zif23 regulated, test: between and after
creator: Ronny Krashinsky
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:16Z
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is between the -10 and -35 sites, and a symmetric Zif23 dimer target site is after the -10 site.
mutable notes: consensus sequences based on [Jensen98].
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus
sequence annotation: -35
sequence annotation: Zif23
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R2002_sequence/1

consensus
type: SequenceAnnotation
persistent identity: annotation318174
display id: annotation318174
version: 1
top level: Zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318174/range318174/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318174/range318174/1
type: Range
persistent identity: range318174
display id: range318174
version: 1
direction: direction/forward
top level: Zif23
start: 1
end: 9
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318160
display id: annotation318160
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318160/range318160/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318160/range318160/1
type: Range
persistent identity: range318160
display id: range318160
version: 1
direction: direction/forward
top level: Zif23
start: 10
end: 15
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318171
display id: annotation318171
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318171/range318171/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318171/range318171/1
type: Range
persistent identity: range318171
display id: range318171
version: 1
direction: direction/forward
top level: Zif23
start: 20
end: 33
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318164
display id: annotation318164
version: 1
top level: Zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318164/range318164/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318164/range318164/1
type: Range
persistent identity: range318164
display id: range318164
version: 1
direction: direction/forward
top level: Zif23
start: 39
end: 52
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318163
display id: annotation318163
version: 1
top level: Zif23
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R2002/annotation318163/range318163/1

https://synbiohub.org/public/igem/BBa_R2002/annotation318163/range318163/1
type: Range
persistent identity: range318163
display id: range318163
version: 1
direction: direction/forward
top level: Zif23
start: 33
end: 38
orientation: inline

BBa_K118011
type: ComponentDefinition
persistent identity: BBa K118011
display id: BBa_K118011
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: PcstA (glucose-repressible promoter)
creator: Andrew Hall
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
discontinued: false
dominant: true
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
mutable notes: No special considerations
mutable provenance: ''Eschaerichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: cAMP receptor protein binding site
sequence annotation: RNA polymerase binding site
sequence: https://synbiohub.org/public/igem/BBa_K118011_sequence/1

cAMP receptor protein binding site
type: SequenceAnnotation
persistent identity: annotation1972435
display id: annotation1972435
version: 1
top level: BBa_K118011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1

https://synbiohub.org/public/igem/BBa_K118011/annotation1972435/range1972435/1
type: Range
persistent identity: range1972435
display id: range1972435
version: 1
direction: direction/forward
top level: BBa_K118011
start: 1
end: 42
orientation: inline

RNA polymerase binding site
type: SequenceAnnotation
persistent identity: annotation1972436
display id: annotation1972436
version: 1
top level: BBa_K118011
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1

https://synbiohub.org/public/igem/BBa_K118011/annotation1972436/range1972436/1
type: Range
persistent identity: range1972436
display id: range1972436
version: 1
direction: direction/forward
top level: BBa_K118011
start: 101
end: 131
orientation: inline

difR
type: ComponentDefinition
persistent identity: BBa I742102
display id: BBa_I742102
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dif site with reverse orientation
creator: Xiaonan Wang
created: 2007-08-07T11:00:00Z
modified: 2015-08-31T04:08:02Z
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1965
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: 'dif' (division induced filamentation) is the recombination site for XerC and XerD recombinases. Once replicated, aligned direct dif repeats are vital to DNA monomerisation during bacterial septation. As with other recombination sites, inverted repeats cause inversion of the DNA in between. However, XerCD requires activation by FtsK and thus two dif-sites (see also part BBa_I742102) enable recombination that is temporally isolated to division.
mutable notes: Synthesised using two oligonucleotides.
mutable provenance: E. coli K12
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: dif site (reverse)
sequence annotation: SacI
sequence: https://synbiohub.org/public/igem/BBa_I742102_sequence/1

dif site (reverse)
type: SequenceAnnotation
persistent identity: annotation1955265
display id: annotation1955265
version: 1
top level: difR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I742102/annotation1955265/range1955265/1

https://synbiohub.org/public/igem/BBa_I742102/annotation1955265/range1955265/1
type: Range
persistent identity: range1955265
display id: range1955265
version: 1
direction: direction/reverse
top level: difR
start: 4
end: 31
orientation: inline

SacI
type: SequenceAnnotation
persistent identity: annotation1940993
display id: annotation1940993
version: 1
top level: difR
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_I742102/annotation1940993/range1940993/1

https://synbiohub.org/public/igem/BBa_I742102/annotation1940993/range1940993/1
type: Range
persistent identity: range1940993
display id: range1940993
version: 1
direction: direction/forward
top level: difR
start: 1
end: 3
orientation: inline

BBa_I716155
type: ComponentDefinition
persistent identity: BBa I716155
display id: BBa_I716155
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: hemD
creator: Kristin Doan
created: 2007-06-27T11:00:00Z
modified: 2015-08-31T04:07:51Z
discontinued: false
dominant: false
experience: experience/None
group u list: _110_
m user id: 0
owner id: 1715
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: This is an enzyme involved in the heme biosynthetic pathway. Also called uroporphyrinogen III synthase.
mutable notes: {{BerkiGEM2007-Biobrick2.0Intro}}
mutable provenance: Genomic MG1655
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I716155_sequence/1

mRFP1
type: ComponentDefinition
persistent identity: BBa I13502
display id: BBa_I13502
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Screening plasmid intermediate
creator: jkm
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Built by Josh as an intermediate in screening plasmid construction.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_I13502_sequence/1

BBa_E1010
type: Component
persistent identity: component1505045
display id: component1505045
version: 1
top level: mRFP1
access: public
definition: mRFP1

BBa_B0034
type: Component
persistent identity: component1505038
display id: component1505038
version: 1
top level: mRFP1
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1505038
display id: annotation1505038
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13502/annotation1505038/range1505038/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13502/annotation1505038/range1505038/1
type: Range
persistent identity: range1505038
display id: range1505038
version: 1
direction: direction/forward
top level: mRFP1
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1505045
display id: annotation1505045
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_I13502/annotation1505045/range1505045/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13502/annotation1505045/range1505045/1
type: Range
persistent identity: range1505045
display id: range1505045
version: 1
direction: direction/forward
top level: mRFP1
start: 19
end: 699
orientation: inline

RFP1
type: ComponentDefinition
persistent identity: BBa I715022
display id: BBa_I715022
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Amino Portion of RFP
creator: Andrew Martens
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:43:58Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: af
mutable notes: afd
mutable provenance: adf
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding sequence
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I715022_sequence/1

Coding sequence
type: SequenceAnnotation
persistent identity: annotation1935381
display id: annotation1935381
version: 1
top level: RFP1
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1

https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1
type: Range
persistent identity: range1935381
display id: range1935381
version: 1
direction: direction/forward
top level: RFP1
start: 1
end: 462
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1935380
display id: annotation1935380
version: 1
top level: RFP1
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1

https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1
type: Range
persistent identity: range1935380
display id: range1935380
version: 1
direction: direction/forward
top level: RFP1
start: 1
end: 3
orientation: inline

BBa_I0406
type: ComponentDefinition
persistent identity: BBa I0406
display id: BBa_I0406
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: R0040.B0034.C0079.E0420
creator: jcbraff
created: 2004-06-08T11:00:00Z
modified: 2015-08-31T04:07:28Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: R0040.B0034.C0079.E0420
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_C0079
component: BBa_R0040
component: BBa_B0012
component: BBa_E0020
component: BBa_B0010
component: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_B0010
sequence annotation: BBa_C0079
sequence: https://synbiohub.org/public/igem/BBa_I0406_sequence/1

BBa_C0079
type: Component
persistent identity: component958690
display id: component958690
version: 1
top level: BBa_I0406
access: public
definition: lasr

BBa_R0040
type: Component
persistent identity: component958669
display id: component958669
version: 1
top level: BBa_I0406
access: public
definition: https://synbiohub.org/public/igem/BBa_R0040/1

BBa_B0012
type: Component
persistent identity: component958713
display id: component958713
version: 1
top level: BBa_I0406
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_E0020
type: Component
persistent identity: component958698
display id: component958698
version: 1
top level: BBa_I0406
access: public
definition: https://synbiohub.org/public/igem/BBa_E0020/1

BBa_B0010
type: Component
persistent identity: component958703
display id: component958703
version: 1
top level: BBa_I0406
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component958696
display id: component958696
version: 1
top level: BBa_I0406
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_B0034
type: Component
persistent identity: component958677
display id: component958677
version: 1
top level: BBa_I0406
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation958698
display id: annotation958698
version: 1
top level: BBa_I0406
location: https://synbiohub.org/public/igem/BBa_I0406/annotation958698/range958698/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_I0406/annotation958698/range958698/1
type: Range
persistent identity: range958698
display id: range958698
version: 1
direction: direction/forward
top level: BBa_I0406
start: 888
end: 1610
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation958677
display id: annotation958677
version: 1
top level: BBa_I0406
location: https://synbiohub.org/public/igem/BBa_I0406/annotation958677/range958677/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0406/annotation958677/range958677/1
type: Range
persistent identity: range958677
display id: range958677
version: 1
direction: direction/forward
top level: BBa_I0406
start: 63
end: 74
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation958713
display id: annotation958713
version: 1
top level: BBa_I0406
location: https://synbiohub.org/public/igem/BBa_I0406/annotation958713/range958713/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0406/annotation958713/range958713/1
type: Range
persistent identity: range958713
display id: range958713
version: 1
direction: direction/forward
top level: BBa_I0406
start: 1707
end: 1747
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation958696
display id: annotation958696
version: 1
top level: BBa_I0406
location: https://synbiohub.org/public/igem/BBa_I0406/annotation958696/range958696/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0406/annotation958696/range958696/1
type: Range
persistent identity: range958696
display id: range958696
version: 1
direction: direction/forward
top level: BBa_I0406
start: 870
end: 881
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation958669
display id: annotation958669
version: 1
top level: BBa_I0406
location: https://synbiohub.org/public/igem/BBa_I0406/annotation958669/range958669/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I0406/annotation958669/range958669/1
type: Range
persistent identity: range958669
display id: range958669
version: 1
direction: direction/forward
top level: BBa_I0406
start: 1
end: 54
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation958703
display id: annotation958703
version: 1
top level: BBa_I0406
location: https://synbiohub.org/public/igem/BBa_I0406/annotation958703/range958703/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0406/annotation958703/range958703/1
type: Range
persistent identity: range958703
display id: range958703
version: 1
direction: direction/forward
top level: BBa_I0406
start: 1619
end: 1698
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation958690
display id: annotation958690
version: 1
top level: BBa_I0406
location: https://synbiohub.org/public/igem/BBa_I0406/annotation958690/range958690/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_I0406/annotation958690/range958690/1
type: Range
persistent identity: range958690
display id: range958690
version: 1
direction: direction/forward
top level: BBa_I0406
start: 81
end: 836
orientation: inline

BBa_I13977
type: ComponentDefinition
persistent identity: BBa I13977
display id: BBa_I13977
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: intermediate for lac relaxation oscillator
creator: jasonk
created: 2004-10-04T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 2
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: intermediate part for the lac version of the synchronized relaxation oscillator
mutable notes: Sequence verified, however there is an N at position 1021, however upon looking at the actual sequence trace it looks like it is most likely the correct base.
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_S3511
component: BBa_I13976
sequence annotation: BBa_S3511
sequence annotation: BBa_I13976
sequence: https://synbiohub.org/public/igem/BBa_I13977_sequence/1

BBa_S3511
type: Component
persistent identity: component2229895
display id: component2229895
version: 1
top level: BBa_I13977
access: public
definition: https://synbiohub.org/public/igem/BBa_S3511/1

BBa_I13976
type: Component
persistent identity: component2229874
display id: component2229874
version: 1
top level: BBa_I13977
access: public
definition: https://synbiohub.org/public/igem/BBa_I13976/1

BBa_S3511
type: SequenceAnnotation
persistent identity: annotation2229895
display id: annotation2229895
version: 1
top level: BBa_I13977
location: https://synbiohub.org/public/igem/BBa_I13977/annotation2229895/range2229895/1
component: BBa_S3511

https://synbiohub.org/public/igem/BBa_I13977/annotation2229895/range2229895/1
type: Range
persistent identity: range2229895
display id: range2229895
version: 1
direction: direction/reverse
top level: BBa_I13977
start: 989
end: 2083
orientation: inline

BBa_I13976
type: SequenceAnnotation
persistent identity: annotation2229874
display id: annotation2229874
version: 1
top level: BBa_I13977
location: https://synbiohub.org/public/igem/BBa_I13977/annotation2229874/range2229874/1
component: BBa_I13976

https://synbiohub.org/public/igem/BBa_I13977/annotation2229874/range2229874/1
type: Range
persistent identity: range2229874
display id: range2229874
version: 1
direction: direction/reverse
top level: BBa_I13977
start: 1
end: 980
orientation: inline

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (LasR & PAI regulated)
creator: Alvin Carter Powers (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Binding region for LasR protein (positive regulation)
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OP1
sequence annotation: OP2
sequence annotation: -10
sequence annotation: -35
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
direction: direction/forward
top level: LasR+PAI
start: 106
end: 125
orientation: inline

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
direction: direction/forward
top level: LasR+PAI
start: 46
end: 63
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
direction: direction/forward
top level: LasR+PAI
start: 140
end: 145
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
direction: direction/forward
top level: LasR+PAI
start: 117
end: 122
orientation: inline

BBa_K103003
type: ComponentDefinition
persistent identity: BBa K103003
display id: BBa_K103003
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: B domain of Staphylococcal protein A
creator: Michael Lower
created: 2008-09-13T11:00:00Z
modified: 2015-05-08T01:08:46Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _180_
m user id: 0
owner id: 2582
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: protein A from Staphylococcus aureus. Also known as part of Tap-Tag
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SacI
sequence annotation: NdeI
sequence annotation: BamHI
sequence annotation: ATG
sequence annotation: B-domain of protein A
sequence: https://synbiohub.org/public/igem/BBa_K103003_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1989823
display id: annotation1989823
version: 1
top level: BBa_K103003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K103003/annotation1989823/range1989823/1

https://synbiohub.org/public/igem/BBa_K103003/annotation1989823/range1989823/1
type: Range
persistent identity: range1989823
display id: range1989823
version: 1
direction: direction/forward
top level: BBa_K103003
start: 230
end: 235
orientation: inline

NdeI
type: SequenceAnnotation
persistent identity: annotation1989822
display id: annotation1989822
version: 1
top level: BBa_K103003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K103003/annotation1989822/range1989822/1

https://synbiohub.org/public/igem/BBa_K103003/annotation1989822/range1989822/1
type: Range
persistent identity: range1989822
display id: range1989822
version: 1
direction: direction/forward
top level: BBa_K103003
start: 3
end: 8
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1986760
display id: annotation1986760
version: 1
top level: BBa_K103003
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_K103003/annotation1986760/range1986760/1

https://synbiohub.org/public/igem/BBa_K103003/annotation1986760/range1986760/1
type: Range
persistent identity: range1986760
display id: range1986760
version: 1
direction: direction/forward
top level: BBa_K103003
start: 9
end: 14
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation1975845
display id: annotation1975845
version: 1
top level: BBa_K103003
role: SO:0000318
role: feature/start
location: https://synbiohub.org/public/igem/BBa_K103003/annotation1975845/range1975845/1

https://synbiohub.org/public/igem/BBa_K103003/annotation1975845/range1975845/1
type: Range
persistent identity: range1975845
display id: range1975845
version: 1
direction: direction/forward
top level: BBa_K103003
start: 6
end: 9
orientation: inline

B-domain of protein A
type: SequenceAnnotation
persistent identity: annotation1975846
display id: annotation1975846
version: 1
top level: BBa_K103003
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_K103003/annotation1975846/range1975846/1

https://synbiohub.org/public/igem/BBa_K103003/annotation1975846/range1975846/1
type: Range
persistent identity: range1975846
display id: range1975846
version: 1
direction: direction/forward
top level: BBa_K103003
start: 6
end: 233
orientation: inline

zif23
type: ComponentDefinition
persistent identity: BBa C2001
display id: BBa_C2001
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli)
creator: Drew Endy
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:25Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Variant of Zif23-GCN4 (see BBa_C2000). CDS has been optimized (via VNTI) for expression in enteric bacteria. ssrA tag (LVA) added.
mutable notes: This protein should form a dimer and bind a specific operator site, thus repressing transcription.
mutable provenance: Protein Engineering Gods from the Pabo Lab
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Finger 3
sequence annotation: linker
sequence annotation: GCN4: leucine zipper
sequence annotation: Recognition site
sequence annotation: LVA
sequence annotation: Finger 2
sequence annotation: Help:Barcodes
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_C2001_sequence/1

Finger 3
type: SequenceAnnotation
persistent identity: annotation318103
display id: annotation318103
version: 1
top level: zif23
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318103/range318103/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318103/range318103/1
type: Range
persistent identity: range318103
display id: range318103
version: 1
direction: direction/forward
top level: zif23
start: 91
end: 150
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation318104
display id: annotation318104
version: 1
top level: zif23
role: SO:0000110
role: feature/misc
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318104/range318104/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318104/range318104/1
type: Range
persistent identity: range318104
display id: range318104
version: 1
direction: direction/forward
top level: zif23
start: 151
end: 165
orientation: inline

GCN4: leucine zipper
type: SequenceAnnotation
persistent identity: annotation318105
display id: annotation318105
version: 1
top level: zif23
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318105/range318105/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318105/range318105/1
type: Range
persistent identity: range318105
display id: range318105
version: 1
direction: direction/forward
top level: zif23
start: 166
end: 261
orientation: inline

Recognition site
type: SequenceAnnotation
persistent identity: annotation318109
display id: annotation318109
version: 1
top level: zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318109/range318109/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318109/range318109/1
type: Range
persistent identity: range318109
display id: range318109
version: 1
direction: direction/forward
top level: zif23
start: 127
end: 147
orientation: inline

Recognition site
type: SequenceAnnotation
persistent identity: annotation318108
display id: annotation318108
version: 1
top level: zif23
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318108/range318108/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318108/range318108/1
type: Range
persistent identity: range318108
display id: range318108
version: 1
direction: direction/forward
top level: zif23
start: 43
end: 63
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation318106
display id: annotation318106
version: 1
top level: zif23
role: SO:0000324
role: feature/tag
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318106/range318106/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318106/range318106/1
type: Range
persistent identity: range318106
display id: range318106
version: 1
direction: direction/forward
top level: zif23
start: 262
end: 294
orientation: inline

Finger 2
type: SequenceAnnotation
persistent identity: annotation318101
display id: annotation318101
version: 1
top level: zif23
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318101/range318101/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318101/range318101/1
type: Range
persistent identity: range318101
display id: range318101
version: 1
direction: direction/forward
top level: zif23
start: 4
end: 90
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214011
display id: annotation2214011
version: 1
top level: zif23
role: SO:0000807
role: feature/barcode
location: https://synbiohub.org/public/igem/BBa_C2001/annotation2214011/range2214011/1

https://synbiohub.org/public/igem/BBa_C2001/annotation2214011/range2214011/1
type: Range
persistent identity: range2214011
display id: range2214011
version: 1
direction: direction/forward
top level: zif23
start: 301
end: 325
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation318107
display id: annotation318107
version: 1
top level: zif23
role: SO:0000319
role: feature/stop
location: https://synbiohub.org/public/igem/BBa_C2001/annotation318107/range318107/1

https://synbiohub.org/public/igem/BBa_C2001/annotation318107/range318107/1
type: Range
persistent identity: range318107
display id: range318107
version: 1
direction: direction/forward
top level: zif23
start: 295
end: 300
orientation: inline

BBa_I13002
type: ComponentDefinition
persistent identity: BBa I13002
display id: BBa_I13002
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: RBS B0031 w/ YFP (-LVA) TT
creator: Jay Blumling, Debra Lin, Madeleine Sheldon-dante, Fred Tan, MIT SMUG
created: 2004-06-27T11:00:00Z
modified: 2015-08-31T04:07:32Z
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 101
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: -- No description --
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0010
component: BBa_E0030
component: BBa_B0012
component: BBa_B0031
sequence annotation: BBa_B0012
sequence annotation: BBa_B0031
sequence annotation: BBa_B0010
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_I13002_sequence/1

BBa_B0010
type: Component
persistent identity: component943459
display id: component943459
version: 1
top level: BBa_I13002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_E0030
type: Component
persistent identity: component943454
display id: component943454
version: 1
top level: BBa_I13002
access: public
definition: eyfp

BBa_B0012
type: Component
persistent identity: component943469
display id: component943469
version: 1
top level: BBa_I13002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0031
type: Component
persistent identity: component943452
display id: component943452
version: 1
top level: BBa_I13002
access: public
definition: https://synbiohub.org/public/igem/BBa_B0031/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943469
display id: annotation943469
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943469/range943469/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13002/annotation943469/range943469/1
type: Range
persistent identity: range943469
display id: range943469
version: 1
direction: direction/forward
top level: BBa_I13002
start: 840
end: 880
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation943452
display id: annotation943452
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943452/range943452/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_I13002/annotation943452/range943452/1
type: Range
persistent identity: range943452
display id: range943452
version: 1
direction: direction/forward
top level: BBa_I13002
start: 1
end: 14
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943459
display id: annotation943459
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943459/range943459/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13002/annotation943459/range943459/1
type: Range
persistent identity: range943459
display id: range943459
version: 1
direction: direction/forward
top level: BBa_I13002
start: 752
end: 831
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation943454
display id: annotation943454
version: 1
top level: BBa_I13002
location: https://synbiohub.org/public/igem/BBa_I13002/annotation943454/range943454/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13002/annotation943454/range943454/1
type: Range
persistent identity: range943454
display id: range943454
version: 1
direction: direction/forward
top level: BBa_I13002
start: 21
end: 743
orientation: inline

RhI
type: ComponentDefinition
persistent identity: BBa I14017
display id: BBa_I14017
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: P(Rhl)
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
created: 2004-08-01T11:00:00Z
modified: 2015-08-31T04:07:37Z
discontinued: false
dominant: true
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: RhlR and C4HSL regulated promoter.
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: RhlR
sequence annotation: -35
sequence annotation: modified P(Rhl)
sequence: https://synbiohub.org/public/igem/BBa_I14017_sequence/1

RhlR
type: SequenceAnnotation
persistent identity: annotation1043005
display id: annotation1043005
version: 1
top level: RhI
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043005/range1043005/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043005/range1043005/1
type: Range
persistent identity: range1043005
display id: range1043005
version: 1
direction: direction/forward
top level: RhI
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1043007
display id: annotation1043007
version: 1
top level: RhI
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043007/range1043007/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043007/range1043007/1
type: Range
persistent identity: range1043007
display id: range1043007
version: 1
direction: direction/forward
top level: RhI
start: 21
end: 50
orientation: inline

modified P(Rhl)
type: SequenceAnnotation
persistent identity: annotation1043006
display id: annotation1043006
version: 1
top level: RhI
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_I14017/annotation1043006/range1043006/1

https://synbiohub.org/public/igem/BBa_I14017/annotation1043006/range1043006/1
type: Range
persistent identity: range1043006
display id: range1043006
version: 1
direction: direction/forward
top level: RhI
start: 1
end: 51
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Promoter (AraC regulated)
creator: Sara Neves (Fighting Darwins)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: c-amp2
sequence annotation: araO1
sequence annotation: c-amp1
sequence annotation: ara1 and ara2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
direction: direction/forward
top level: AraC
start: 113
end: 118
orientation: inline

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
direction: direction/forward
top level: AraC
start: 4
end: 29
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
role: SO:0000057
role: feature/operator
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
direction: direction/forward
top level: AraC
start: 6
end: 44
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
direction: direction/forward
top level: AraC
start: 43
end: 72
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
role: feature/binding
role: SO:0001091
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
direction: direction/forward
top level: AraC
start: 73
end: 101
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
role: SO:0000167
role: feature/promoter
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
direction: direction/forward
top level: AraC
start: 136
end: 141
orientation: inline

BBa_J52019
type: ComponentDefinition
persistent identity: BBa J52019
display id: BBa_J52019
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: dnTraf6
creator: Matej Skocaj
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
discontinued: false
dominant: false
group u list: _80_
m user id: 0
owner id: 857
owning group id: 80
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: It is an imortant protein in signaling cascade through TLr receptors (MyD88 dependent pathway).
mutable notes: None.
mutable provenance: pDeNy
owned by: james
owned by: myers
star: false
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: dnTraf6
sequence: https://synbiohub.org/public/igem/BBa_J52019_sequence/1

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903257
display id: annotation1903257
version: 1
top level: BBa_J52019
role: feature/cds
role: SO:0000316
location: https://synbiohub.org/public/igem/BBa_J52019/annotation1903257/range1903257/1

https://synbiohub.org/public/igem/BBa_J52019/annotation1903257/range1903257/1
type: Range
persistent identity: range1903257
display id: range1903257
version: 1
direction: direction/forward
top level: BBa_J52019
start: 1
end: 714
orientation: inline

dnTraf6
type: SequenceAnnotation
persistent identity: annotation1903258
display id: annotation1903258
version: 1
top level: BBa_J52019
role: SO:0000804
role: feature/BioBrick
location: https://synbiohub.org/public/igem/BBa_J52019/annotation1903258/range1903258/1

https://synbiohub.org/public/igem/BBa_J52019/annotation1903258/range1903258/1
type: Range
persistent identity: range1903258
display id: range1903258
version: 1
direction: direction/forward
top level: BBa_J52019
start: 1
end: 714
orientation: inline

BBa_I1468
type: ComponentDefinition
persistent identity: BBa I1468
display id: BBa_I1468
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: CinR protein generator
creator: Barry Canton
created: 2004-08-09T11:00:00Z
modified: 2015-08-31T04:07:38Z
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: false
mutable description: Elowitz RBS. CinR CDS (no LVA tag) and terminator. Same as I0468 but no LVA tag
owned by: james
owned by: myers
star: false
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_C0177
sequence annotation: BBa_B0012
sequence annotation: BBa_C0177
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_I1468_sequence/1

BBa_B0012
type: Component
persistent identity: component1040175
display id: component1040175
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_B0010
type: Component
persistent identity: component1040165
display id: component1040165
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0034
type: Component
persistent identity: component1040158
display id: component1040158
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_C0177
type: Component
persistent identity: component1040160
display id: component1040160
version: 1
top level: BBa_I1468
access: public
definition: https://synbiohub.org/public/igem/BBa_C0177/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1040175
display id: annotation1040175
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040175/range1040175/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I1468/annotation1040175/range1040175/1
type: Range
persistent identity: range1040175
display id: range1040175
version: 1
direction: direction/forward
top level: BBa_I1468
start: 844
end: 884
orientation: inline

BBa_C0177
type: SequenceAnnotation
persistent identity: annotation1040160
display id: annotation1040160
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040160/range1040160/1
component: BBa_C0177

https://synbiohub.org/public/igem/BBa_I1468/annotation1040160/range1040160/1
type: Range
persistent identity: range1040160
display id: range1040160
version: 1
direction: direction/forward
top level: BBa_I1468
start: 19
end: 747
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1040165
display id: annotation1040165
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040165/range1040165/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I1468/annotation1040165/range1040165/1
type: Range
persistent identity: range1040165
display id: range1040165
version: 1
direction: direction/forward
top level: BBa_I1468
start: 756
end: 835
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1040158
display id: annotation1040158
version: 1
top level: BBa_I1468
location: https://synbiohub.org/public/igem/BBa_I1468/annotation1040158/range1040158/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I1468/annotation1040158/range1040158/1
type: Range
persistent identity: range1040158
display id: range1040158
version: 1
direction: direction/forward
top level: BBa_I1468
start: 1
end: 12
orientation: inline

BBa_K137042
type: ComponentDefinition
persistent identity: BBa K137042
display id: BBa_K137042
version: 1
was generated by: https://synbiohub.org/public/igem/igem2sbol/1
description: Regulatory SSM device: Promoter with (TA)10 repeat expressing GFP
creator: Allen Lin
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
part status: Released HQ 2013
sample status: In stock
status: status/Available
bookmark: true
mutable description: Promoter with (TA)10 repeat upstream of RBS + GFP + TT
mutable notes: Promoter with (TA)10 repeat upstream of RBS + GFP + TT
mutable provenance: K137029 + I13504
owned by: james
owned by: myers
star: true
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0034
component: BBa_E0040
component: BBa_B0010
component: BBa_B0012
component: BBa_K137029
sequence annotation: BBa_K137029
sequence annotation: BBa_B0012
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K137042_sequence/1

BBa_B0034
type: Component
persistent identity: component1967971
display id: component1967971
version: 1
top level: BBa_K137042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0034/1

BBa_E0040
type: Component
persistent identity: component1967973
display id: component1967973
version: 1
top level: BBa_K137042
access: public
definition: https://synbiohub.org/public/igem/BBa_E0040/1

BBa_B0010
type: Component
persistent identity: component1967974
display id: component1967974
version: 1
top level: BBa_K137042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0010/1

BBa_B0012
type: Component
persistent identity: component1967976
display id: component1967976
version: 1
top level: BBa_K137042
access: public
definition: https://synbiohub.org/public/igem/BBa_B0012/1

BBa_K137029
type: Component
persistent identity: component1967969
display id: component1967969
version: 1
top level: BBa_K137042
access: public
definition: https://synbiohub.org/public/igem/BBa_K137029/1

BBa_K137029
type: SequenceAnnotation
persistent identity: annotation1967969
display id: annotation1967969
version: 1
top level: BBa_K137042
location: https://synbiohub.org/public/igem/BBa_K137042/annotation1967969/range1967969/1
component: BBa_K137029

https://synbiohub.org/public/igem/BBa_K137042/annotation1967969/range1967969/1
type: Range
persistent identity: range1967969
display id: range1967969
version: 1
direction: direction/reverse
top level: BBa_K137042
start: 1
end: 39
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1967976
display id: annotation1967976
version: 1
top level: BBa_K137042
location: https://synbiohub.org/public/igem/BBa_K137042/annotation1967976/range1967976/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K137042/annotation1967976/range1967976/1
type: Range
persistent identity: range1967976
display id: range1967976
version: 1
direction: direction/forward
top level: BBa_K137042
start: 882
end: 922
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1967973
display id: annotation1967973
version: 1
top level: BBa_K137042
location: https://synbiohub.org/public/igem/BBa_K137042/annotation1967973/range1967973/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K137042/annotation1967973/range1967973/1
type: Range
persistent identity: range1967973
display id: range1967973
version: 1
direction: direction/reverse
top level: BBa_K137042
start: 66
end: 785
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1967974
display id: annotation1967974
version: 1
top level: BBa_K137042
location: https://synbiohub.org/public/igem/BBa_K137042/annotation1967974/range1967974/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K137042/annotation1967974/range1967974/1
type: Range
persistent identity: range1967974
display id: range1967974
version: 1
direction: direction/forward
top level: BBa_K137042
start: 794
end: 873
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1967971
display id: annotation1967971
version: 1
top level: BBa_K137042
location: https://synbiohub.org/public/igem/BBa_K137042/annotation1967971/range1967971/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K137042/annotation1967971/range1967971/1
type: Range
persistent identity: range1967971
display id: range1967971
version: 1
direction: direction/reverse
top level: BBa_K137042
start: 48
end: 59
orientation: inline