##gff-version 3
##sequence-region IBMc428 9 5139
IBMc428	.	CDS	2281	2883	.	+	0	ID=annotation4;Name=PhlF
IBMc428	.	origin_of_replication	4315	5139	.	+	0	ID=annotation16;Name=p15A origin of replication
IBMc428	.	biological_region	1494	1494	.	+	0	ID=annotation2;Name=RFC10 Prefix (full)
IBMc428	.	primer_binding_site	3849	3868	.	-	0	ID=annotation3;Name=VR
IBMc428	.	CDS	98	1291	.	+	0	ID=annotation5;Name=tetR
IBMc428	.	terminator	3148	3200	.	+	0	ID=annotation15;Name=L3S3P21
IBMc428	.	promoter	9	97	.	+	0	ID=annotation19;Name=tetR promoter
IBMc428	.	promoter	2199	2252	.	+	0	ID=annotation1;Name=PlacIQ
IBMc428	.	CDS	3391	3552	.	+	0	ID=annotation6;Name=M86C
IBMc428	.	CDS	3556	3669	.	+	0	ID=annotation18;Name=SrpR
IBMc428	.	promoter	3220	3285	.	+	0	ID=annotation10;Name=PPhlF
IBMc428	.	terminator	2134	2194	.	+	0	ID=annotation8;Name=L3S2P21
IBMc428	.	primer_binding_site	1371	1390	.	+	0	ID=annotation9;Name=VF2
IBMc428	.	terminator	2893	3147	.	+	0	ID=annotation7;Name=IOT
IBMc428	.	terminator	3681	3733	.	+	0	ID=annotation12;Name=ECK120033736
IBMc428	.	biological_region	3734	3754	.	+	0	ID=annotation14;Name=RFC10 Suffix
IBMc428	.	ribosome_entry_site	2253	2280	.	+	0	ID=annotation11;Name=phl2
IBMc428	.	insulator	3286	3360	.	+	0	ID=annotation0;Name=RiboJ
IBMc428	.	promoter	2036	2133	.	-	0	ID=annotation13;Name=cat promoter
IBMc428	.	CDS	1517	2035	.	-	0	ID=annotation17;Name=cat (BsmBI-)
IBMc428	.	ribosome_entry_site	3369	3381	.	+	0	ID=annotation20;Name=BBa_B0032
>IBMc428
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAA
CTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAAC
ACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTC
AGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGA
AATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGG
TCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG
TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCT
GAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCC
ctcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtccaatggcggcgcgccatcg
aatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcatgggggtgaatatggcacgtac
cccgagccgtagcagcattggtagcctgcgtagtccgcatacccataaagcaattctgaccagcaccattgaaatcctg
aaagaatgtggttatagcggtctgagcattgaaagcgtggcacgtcgcgccggtgcaggcaaaccgaccatttatcgtt
ggtggaccaacaaagcagcactgattgccgaagtgtatgaaaatgaaatcgaacaggtacgtaaatttccggatttggg
tagctttaaagccgatctggattttctgctgcataatctgtggaaagtttggcgtgaaaccatttgtggtgaagcattt
cgttgtgttattgcagaagcacagttggaccctgtaaccctgacccaactgaaagatcagtttatggaacgtcgtcgtg
agataccgaaaaaactggttgaagatgccattagcaatggtgaactgccgaaagatatcaatcgtgaactgctgctgga
tatgatttttggtttttgttggtatcgcctgctgaccgaacagttgaccgttgaacaggatattgaagaatttaccttc
ctgctgattaatggtgtttgtccgggtacacagtgttgataaggatcctaattggtaacgaatcagacaattgacggct
cgagggagtagcatagggtttgcagaatccctgcttcgtccatttgacaggcacattatgcatcgatgataagctgtca
aacatgagcagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatat
cgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcaaatattttatctgccaattattgaag
gcctccctaacggggggcctttttttgtttctggtctcccgcttaacgatcgttggctgcgacgtacggtggaatctga
ttcgttaccaattgacatgatacgaaacgtaccgtatcgttaaggtagctgtcaccggatgtgctttccggtctgatga
gtccgtgaggacgaaacagcctctacaaataattttgtttaatactagagtcacacaggaaagtactagATGagctcct
ctttacaattggcaccagaaatagaaaagttgcctcagagtgatatttactgggaccccatcgtttctattacggagac
tggagtcgaagaggtttttgatttgactgtgccaggactacgtaactttgtcgccaatgacatcattgtacataacTCA
CAGCAGGCACAGATTATCAAAGTTGCACTGGGTAGCTTTTGGGCACTGCTGCGTGAACCGCCTCGTTTTCTGCTGTGTG
AAGAAGCACAGATTAAACAGGTGAAATCCTTCGAATAAtactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTC
CGGGGGCTTTtttattgcgctactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagt
caaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcag
ataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgc
aggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgc
ggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaacta
tggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactc
atatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatg
accaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttg
gtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaa
ctctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcat
gacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggac
tcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaa
ctgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaa
aggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccac
cactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctct
cacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagt
cgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtg
cagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactcc
gctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaa
agtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaa
cggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttg
tgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaa
cagtaatacaaggggtgtt