##gff-version 3
##sequence-region IBMc371 9 6003
IBMc371	.	CDS	2731	3033	.	+	0	ID=annotation5;Name=ECF20 1-101
IBMc371	.	origin_of_replication	5179	6003	.	+	0	ID=annotation10;Name=p15A origin of replication
IBMc371	.	promoter	2422	2705	.	+	0	ID=annotation1;Name=PBAD(SapI-)
IBMc371	.	CDS	4048	4254	.	+	0	ID=annotation3;Name=M86(85-154)
IBMc371	.	CDS	4258	4533	.	+	0	ID=annotation12;Name=ECF20 102-193
IBMc371	.	CDS	3037	3291	.	+	0	ID=annotation14;Name=M86(1-76)
IBMc371	.	CDS	3301	4044	.	+	0	ID=annotation4;Name=ER-LBD
IBMc371	.	CDS	1516	2394	.	-	0	ID=annotation8;Name=araC
IBMc371	.	promoter	9	97	.	+	0	ID=annotation6;Name=tetR promoter
IBMc371	.	ribosome_entry_site	2714	2724	.	+	0	ID=annotation15;Name=BBa_B0033
IBMc371	.	biological_region	4598	4618	.	+	0	ID=annotation9;Name=RFC10 Suffix
IBMc371	.	primer_binding_site	4713	4732	.	-	0	ID=annotation0;Name=VR
IBMc371	.	biological_region	1494	1515	.	+	0	ID=annotation11;Name=RFC10 Prefix (full)
IBMc371	.	primer_binding_site	1371	1390	.	+	0	ID=annotation13;Name=VF2
IBMc371	.	CDS	98	1291	.	+	0	ID=annotation2;Name=tetR
IBMc371	.	terminator	4545	4597	.	+	0	ID=annotation7;Name=ECK120033736
>IBMc371
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggc
tggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgat
aggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccct
aactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgc
tgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaat
cgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccg
gcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaa
atattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagc
ctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgt
ccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataa
aaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcag
gggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcaga
cattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaac
aaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaa
ctctctactgtttctccattactagagtcacacaggactactagatgaatgaaaccgatcctgatctggaactgctgaa
acgtattggtaataatgatgcacaggccgttaaagaaatggttacccgtaaactgcctcgtctgctggcactggcaagt
cgcctgctgggtgatgcagatgaagcacgtgatattgcacaagaaagttttctgcgcatttggaaacaggcagcaagct
ggcgtagcgaacaggcacgttttgatacctggctgcatcgtgttgcactgaatctgtgttatgatcgtctgcgtcgtcg
taaagaacatgtgccggttgatagcgaacatGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaa
agagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaat
cagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaa
ggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctTGGAAATAGCCTGGCACTGAGC
CTGACCGCAGATCAGATGGTTAGCGCACTGCTGGATGCAGAACCGCCTATTCTGTATAGCGAATATGATCCGACACGTC
CGTTTAGCGAAGCAAGCATGATGGGTCTGCTGACCAATCTGGCAGATCGTGAACTGGTTCATATGATTAATTGGGCAAA
ACGTGTTCCGGGTTTTGTTGATCTGACCCTGCATGATCAGGTTCATCTGCTGGAATGTGCATGGCTGGAAATTCTGATG
ATTGGTCTGGTTTGGCGTAGCATGGAACATCCGGGTAAACTGCTGTTTGCACCGAATCTGCTGCTGGATCGTAATCAGG
GTAAATGTGTTGAAGGTATGGTGGAAATCTTTGATATGCTGCTGGCAACCAGCAGCCGTTTTCGTATGATGAATCTGCA
AGGTGAAGAATTTGTTTGCCTGAAAAGCATTATTCTGCTGAACAGCGGTGTTTATACCTTTCTGAGCAGCACCCTGAAA
AGTCTGGAAGAAAAAGATCATATCCATCGTGTGCTGGATAAAATCACCGATACACTGATTCATCTGATGGCAAAAGCCG
GTCTGACCTTACAGCAGCAGCATCAGCGTCTGGCACAACTGCTGCTGATTCTGAGCCATATTCGTCATATGAGCAATAA
AGGTATGGAACACCTGTACAGCATGAAATGCAAAAATGTTGTTCCGCTGTATGACCTGCTGTTAGAAATGCTGGACGCA
CATCGTCTGCATGCATCAgagctatctttaaaagagcatattgctctaccccgtaaactagaaagctcctctttacaat
tggcaccagaaatagaaaagttgcctcagagtgatatttactgggaccccatcgtttctattacggagactggagtcga
agaggtttttgatttgactgtgccaggactacgtaactttgtcgccaatgacatcattgtacataacTCAgcctgtgaa
gcactggatacccgtccggcaccggatgaacagctggaagcaagcgcacagagccgtcgtatggcacaggcactggatc
agctgccggatcgtcagcgtgaagcaattgttctgcagtattatcaagaactgagcaataccgaagcagcagcactgat
gcaaattagcgttgaagccctggaaagcctgctgagccgtgcacgtcgtaatctgcgtagccatctggccgaagcaccg
ggtgcagatctgagcggtcgtcgcaaaccgtaatactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGG
GCTTTtttattgcgctactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaa
gcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaa
aaaaatccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggac
cacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtat
cattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggat
gaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatata
tactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaa
aatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctg
cgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctt
tgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgactt
caagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaag
acgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcc
tacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggca
ggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactg
atttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcactt
ccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaac
gaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagcc
ttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctag
cgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtga
gggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtc
tgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtct
caaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagta
atacaaggggtgtt