##gff-version 3
##sequence-region IBMc334 9 7921
IBMc334	.	terminator	4216	4305	.	+	0	ID=annotation3;Name=ECK120029600
IBMc334	.	insulator	5530	5604	.	+	0	ID=annotation17;Name=RiboJ
IBMc334	.	promoter	9	97	.	+	0	ID=annotation1;Name=tetR promoter
IBMc334	.	terminator	4314	4370	.	-	0	ID=annotation0;Name=ECK120033737
IBMc334	.	CDS	4399	5058	.	-	0	ID=annotation29;Name=cat (BsmBI-)
IBMc334	.	promoter	5241	5524	.	+	0	ID=annotation20;Name=PBAD(SapI-)
IBMc334	.	terminator	5165	5221	.	+	0	ID=annotation23;Name=L3S2P55
IBMc334	.	ribosome_entry_site	5675	5683	.	+	0	ID=annotation12;Name=RBS for CDS2
IBMc334	.	primer_binding_site	1371	1390	.	+	0	ID=annotation22;Name=VF2
IBMc334	.	ribosome_entry_site	2714	2728	.	+	0	ID=annotation14;Name=BBa_B0030
IBMc334	.	primer_binding_site	6631	6650	.	-	0	ID=annotation19;Name=VR
IBMc334	.	CDS	3884	4204	.	+	0	ID=annotation6;Name=FKBP E coli optimized
IBMc334	.	CDS	98	1291	.	+	0	ID=annotation7;Name=tetR
IBMc334	.	CDS	1516	2394	.	-	0	ID=annotation9;Name=araC
IBMc334	.	promoter	5059	5156	.	-	0	ID=annotation26;Name=cat promoter
IBMc334	.	CDS	5987	6142	.	+	0	ID=annotation5;Name=NpuN(51-102)
IBMc334	.	CDS	2735	3309	.	+	0	ID=annotation28;Name=mCherry*(1-192)
IBMc334	.	CDS	6185	6298	.	+	0	ID=annotation2;Name=NpuC
IBMc334	.	CDS	5693	5971	.	+	0	ID=annotation18;Name=FRB E coli optimized
IBMc334	.	CDS	3680	3862	.	+	0	ID=annotation24;Name=(NpuC1-30(K2E,R6E,K11E))x2
IBMc334	.	CDS	3644	3655	.	+	0	ID=annotation8;Name=Caspase3 cut site
IBMc334	.	origin_of_replication	7097	7921	.	+	0	ID=annotation16;Name=p15A origin of replication
IBMc334	.	CDS	3323	3628	.	+	0	ID=annotation11;Name=NpuN(E52K,E54K,D85K,E89R,E91K)
IBMc334	.	CDS	6434	6451	.	+	0	ID=annotation27;Name=H6
IBMc334	.	biological_region	5605	5689	.	+	0	ID=annotation10;Name=BCD1
IBMc334	.	biological_region	6516	6536	.	+	0	ID=annotation21;Name=RFC10 Suffix
IBMc334	.	biological_region	1494	1515	.	+	0	ID=annotation4;Name=RFC10 Prefix (full)
IBMc334	.	ribosome_entry_site	5624	5631	.	+	0	ID=annotation15;Name=RBS for CDS 1
IBMc334	.	CDS	6302	6433	.	+	0	ID=annotation30;Name=mCherry*(193-236)
IBMc334	.	terminator	6463	6515	.	+	0	ID=annotation25;Name=ECK120033736
IBMc334	.	promoter	2422	2705	.	+	0	ID=annotation31;Name=PBAD(SapI-)
IBMc334	.	CDS	6158	6169	.	+	0	ID=annotation13;Name=Caspase3 cut site
>IBMc334
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggc
tggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgat
aggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccct
aactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgc
tgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaat
cgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccg
gcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaa
atattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagc
ctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgt
ccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataa
aaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcag
gggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcaga
cattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaac
aaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaa
ctctctactgtttctccattactagagattaaagaggagaaatactagatggtgagcaagggcgaagaaGATaacatgg
ccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcga
gggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgg
gacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagc
tgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactc
ctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcag
aagAAAaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcaga
ggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccGTTGGAGCAGA
ATATTGCCTGAGCTATGAAACCGAAATTCTGACCGTTGAATATGGTCTGCTGCCGATTGGTAAAATTGTGGAAAAACGT
ATTGAGTGCACCGTGTATAGCGTGGATAATAACGGTAACATTTATACCCAGCCGGTTGCACAGTGGCATGATCGTGGTA
AACAGAAAGTTTTTGAGTACTGTCTGGAAGATGGTAGCCTGATTCGTGCAACCAAAGATCACAAATTTATGACCGTGGA
TGGTCAGATGCTGCCCATTAAAGAAATTTTTCGTCGTAAACTGGATCTGATGCGTGTTGATAATCTGCCGAATGAAAAT
CTGTATGGTGATGAAGTGGATGGCTATTTTCAGGGTATTGAAATTGCCACCGAAAAATATCTGGGTGAGCAGAACGTTT
ATGATATTGGTGTTGAACGCGATCATAACTTCGCACTGAAAAATGGTGGTTATTTCCAGGGCATCGAAATCGCAACAGA
GAAATACCTGGGCGAACAGAATGTGTATGACATCGGCGTGGAACGTGATCACAATTTTGCCCTGAAGAATGGCGGTAGC
GGTAGCGCAAGCGGAGTTCAGGTAGAGACAATTAGTCCTGGGGATGGCCGCACATTTCCGAAACGCGGCCAAACCTGTG
TGGTACACTATACAGGAATGTTGGAAGATGGCAAAAAGTTTGATTCCAGTCGTGATCGCAACAAGCCTTTTAAATTTAT
GTTGGGAAAACAGGAGGTAATTCGCGGTTGGGAGGAAGGTGTCGCACAAATGTCCGTGGGTCAACGCGCCAAGTTGACG
ATCTCACCTGATTACGCTTACGGAGCCACCGGCCATCCTGGCATTATTCCCCCACATGCCACTTTGGTCTTTGATGTTG
AACTTTTAAAGTTAGAAtaaTACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaagg
tagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCA
GGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGC
AGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGG
CATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACG
TTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAAT
AGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACT
CCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCA
CCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACT
TGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAAC
TGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCC
ATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAA
AGTTGGAACCTCTTACGTGCCTACTAGAGCTCGGTACCAAAGACGAACAATAAGACGCTGAAAAGCGTCTTTTTTCGTT
TTGGTCCgcttaacgatcgttggctgagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttact
ggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaa
aacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgcc
atagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccga
gctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaGGGCC
CAAGTTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCATGCACAGGAGACTTTCT
AatgGCCGTGTTATGGCACGAACAGTGGTATGAGGGTTTGGATGACGCATCGCGCCAGTTCTTCGGAGAACATAACACC
GAAAAAATGTTTGCGGCACTGGAACCTCTTTATGAGATGCTTAAACGTGGGCCTGAAACGTTGCGTGAGATTTCTTTTC
AGAACTCATTTGGGCGTGATTTGAACGATGCCTACGAATGGCTGATGAATTACAAAAAGTCGAAAGATGTATCTAACCT
GAATCAAGCATGGGATATTTACTATAATGTCTTTCGTAAAATCGGCGGTAATGGTAATGGTGGTGAACAAGAAGTGTTT
GAGTATTGTCTGGAAGATGGTAGCCTGATTCGTGCAACCAAAGATCACAAATTTATGACCGTTGATGGTCAGATGCTGC
CGATTGATGAAATTTTTGAACGTGAACTGGATCTGATGCGTGTTGATAATCTGCCGAATGAAAATCTGTATGGTGATGA
AGTGGATGGCTATTTTCAGGGTATCAAAATTGCGACCCGTAAATATCTGGGTAAACAGAACGTTTATGATATTGGTGTG
GAACGCGATCATAACTTTGCACTGAAAAATGGTTTTATCGCCAGCAACTGCTTCAATTCAcagctgcccggcgcctaca
acgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccg
ccactccaccggcggcatggacgagctgtacaagcatcatcaccatcaccactaatactagagaacgcatgagAAAGCC
CCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctactagtagcggccgctgcaggagtcactaagggttagttag
ttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcac
tcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaatagactggatg
gaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccg
gtgagcgtggatctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgac
ggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactg
tcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcc
tttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatc
ttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaag
gttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagttt
agccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcat
gtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcataca
gtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaat
gacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtc
ctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacgg
ctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagcca
tttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatatt
ctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagt
aagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgcc
ccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaact
tttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttat
tcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaa
ctgtctgcttacataaacagtaatacaaggggtgtt