##gff-version 3
##sequence-region IBMc325 9 6175
IBMc325	.	promoter	9	97	.	+	0	ID=annotation8;Name=tetR promoter
IBMc325	.	origin_of_replication	5351	6175	.	+	0	ID=annotation9;Name=p15A origin of replication
IBMc325	.	biological_region	1494	1515	.	+	0	ID=annotation10;Name=RFC10 Prefix (full)
IBMc325	.	CDS	4688	4705	.	+	0	ID=annotation11;Name=H6
IBMc325	.	CDS	98	1291	.	+	0	ID=annotation4;Name=tetR
IBMc325	.	promoter	2422	2705	.	+	0	ID=annotation14;Name=PBAD(SapI-)
IBMc325	.	terminator	4717	4769	.	+	0	ID=annotation5;Name=ECK120033736
IBMc325	.	CDS	1516	2394	.	-	0	ID=annotation6;Name=araC
IBMc325	.	CDS	4556	4687	.	+	0	ID=annotation1;Name=mCherry*(193-236)
IBMc325	.	ribosome_entry_site	2714	2728	.	+	0	ID=annotation3;Name=BBa_B0030
IBMc325	.	primer_binding_site	4885	4904	.	-	0	ID=annotation0;Name=VR
IBMc325	.	biological_region	4770	4790	.	+	0	ID=annotation7;Name=RFC10 Suffix
IBMc325	.	primer_binding_site	1371	1390	.	+	0	ID=annotation12;Name=VF2
IBMc325	.	CDS	2735	3310	.	+	0	ID=annotation13;Name=mCherry*(1-192)
IBMc325	.	CDS	3314	4552	.	+	0	ID=annotation2;Name=Mtu RecA 37R3-2
>IBMc325
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggc
tggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgat
aggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccct
aactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgc
tgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaat
cgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccg
gcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaa
atattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagc
ctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgt
ccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataa
aaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcag
gggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcaga
cattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaac
aaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaa
ctctctactgtttctccattactagagattaaagaggagaaatactagatggtgagcaagggcgaagaaGATaacatgg
ccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcga
gggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgg
gacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagc
tgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactc
ctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcag
aagAAAaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcaga
ggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccGTTGGATGTCT
GGCAGAAGGCACCCGTATTTTTGATCCGGTTACCGGTACAACCCATCGTATTGAAGATGTTGTTGATGGTCGTAAACCG
ATTCATGTTGTTGCAGCAGCAAAAGATGGCACCCTGCTGGCACGTCCGGTTGTTAGCTGGTTTGATCAGGGCACCCGTG
ATGTTATTGGTCTGCGTATTGCCGGTGGTGCAATTGTTTGGGCAACACCGGATCATAAAGTTCTGACCGAATATGGTTG
GCGTGCAGCCGGTGAACTGCGTAAAGGTGATCGTGTTGCAGGTCCTGGTGGTAGCGGTAATAGCCTGGCACTGAGCCTG
ACCGCAGATCAGATGGTTAGCGCACTGCTGGATGCAGAACCGCCTATTCTGTATAGCGAATATGATCCGACCAGTCCGT
TTAGCGAAGCAAGCATGATGGGTCTGCTGACCAATCTGGCAGATCGTGAACTGGTTCATATGATTAATTGGGCAAAACG
TGTTCCGGGTTTTGTTGATCTGACCCTGCATGATCAGGCACATCTGCTGGAACGTGCATGGCTGGAAATTCTGATGATT
GGTCTGGTTTGGCGTAGCATGGAACATCCGGGTAAACTGCTGTTTGCACCGAATCTGCTGCTGGATCGTAATCAGGGTA
AATGTGTTGAAGGTATGGTGGAAATCTTTGATATGCTGCTGGCAACCAGCAGCCGTTTTCGTATGATGAATCTGCAAGG
TGAAGAATTTGTTTGCCTGAAAAGCATTATTCTGCTGAACAGCGGTGTTTATACCTTTCTGAGCAGCACCCTGAAAAGT
CTGGAAGAAAAAGATCATATCCATCGTGCCCTGGATAAAATCACCGATACACTGATTCATCTGATGGCAAAAGCCGGTC
TGACCTTACAGCAGCAGCATCAGCGTCTGGCACAACTGCTGCTGATTCTGAGCCATATTCGTCATATGAGCAATAAAGG
TATGGAACACCTGTACAGCATGAAGTATAAAAACGTTGTGCCGCTGTATGATCTGCTGTTAGAAATGCTGGACGCACAT
CGTCTGCATGCCGGTGGCAGCGGTGCAAGCCGTGTTCAGGCATTTGCAGATGCACTGGATGATAAATTTCTGCACGATA
TGCTGGCCGAAGGTCTGCGTTATAGCGTTATTCGTGAAGTTCTGCCGACACGTCGTGCACGTACCTTTGATCTGGAAGT
TGAAGAACTGCATACCCTGGTTGCAGAAGGTGTTGTTGTTCATAATTGCTCAcagctgcccggcgcctacaacgtcaac
atcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactcca
ccggcggcatggacgagctgtacaagcatcatcaccatcaccactaatactagagaacgcatgagAAAGCCCCCGGAAG
ATCACCTTCCGGGGGCTTTtttattgcgctactagtagcggccgctgcaggagtcactaagggttagttagttagatta
gcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggc
ggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcgga
taaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgt
ggatctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtc
aggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagacca
agtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgat
aatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgag
atcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctg
agctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaa
ccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttcc
gggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagct
tggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccgg
taaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcggg
tttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccg
cggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgct
cgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgac
gcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagt
atacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatc
cagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgcttt
gccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaa
gccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgc
ttacataaacagtaatacaaggggtgtt