##gff-version 3
##sequence-region IBMc259 186 3974
IBMc259	.	terminator	3794	3854	.	+	0	ID=annotation9;Name=L3S2P21
IBMc259	.	origin_of_replication	2240	2462	.	+	0	ID=annotation2;Name=pSC101 ori
IBMc259	.	biological_region	2877	2898	.	+	0	ID=annotation8;Name=RFC10 Prefix (full)
IBMc259	.	biological_region	3855	3875	.	+	0	ID=annotation13;Name=RFC10 Suffix
IBMc259	.	insulator	2994	3068	.	+	0	ID=annotation10;Name=RiboJ10
IBMc259	.	promoter	2918	2993	.	+	0	ID=annotation1;Name=pTet*
IBMc259	.	primer_binding_site	2719	2736	.	+	0	ID=annotation7;Name=pSEVA PS1 Fwd
IBMc259	.	CDS	1242	2192	.	-	0	ID=annotation3;Name=Rep101
IBMc259	.	ribosome_entry_site	3077	3088	.	+	0	ID=annotation6;Name=BBa_B0064
IBMc259	.	primer_binding_site	3934	3950	.	-	0	ID=annotation11;Name=pSEVA PS2 Rev
IBMc259	.	CDS	3095	3790	.	+	0	ID=annotation0;Name=mScarlet-I
IBMc259	.	CDS	186	977	.	+	0	ID=annotation4;Name=SmR
IBMc259	.	terminator	3880	3974	.	+	0	ID=annotation5;Name=lambda t0 terminator
IBMc259	.	terminator	2729	2815	.	-	0	ID=annotation12;Name=rrnB T1 terminator
>IBMc259
aacgcagcggtggtaacggcgcagtggcggttttcatggcttgttatgactgtttttttggggtacagtctatgcctcg
ggcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgatgttatggagcagcaacgatgttacgcagcagggc
agtcgccctaaaacaaagttaaacatcATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTG
GCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCC
ACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTT
TTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCA
TTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGA
GCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCG
GCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGC
CGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAAT
CGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGA
CAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAG
GCGAGATCACCAAGGTAGTCGGCAAATAAGACaaTTGTCCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCG
ATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTA
TCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTC
GCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCTCAGATCCTTCCGTATTTAGCCA
GTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTC
ACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTA
TGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTA
CGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTT
CATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTA
TTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTT
CTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAA
TTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAG
TTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTG
CTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGT
CCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCC
GTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGT
GATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACATGTCCTTTTCCTTTGAGTTGTGGGTATCTG
TAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTT
TTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCC
CTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAA
CAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGAC
CATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAA
TGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAA
CAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAAGCGGATAACAATTT
CACACAGGAGGCCGCCTAGGCCGCGGCCGCGCGaattcgcggccgcttctagaggcttaacgatcgttggctgttttca
gcaggacgcactgacctccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacagcgctcaa
cgggtgtgcttcccgttctgatgagtccgtgaggacgaaagcgcctctacaaataattttgtttaatactagagaaaga
ggggaaatactagatggtgagtaaaggagaagctgtgattaaagagttcatgcgcttcaaagttcacatggagggttct
atgaacggtcacgagttcgagatcgaaggcgaaggcgagggccgtccgtatgaaggcacccagaccgccaaactgaaag
tgactaaaggcggcccgctgcctttttcctgggacatcctgagcccgcaatttatgtacggttctagggcgttcatcaa
acacccagcggatatcccggactattataagcagtcttttccggaaggtttcaagtgggaacgcgtaatgaattttgaa
gatggtggtgccgtgaccgtcactcaggacacctccctggaggatggcaccctgatctataaagttaaactgcgtggta
ctaattttccacctgatggcccggtgatgcagaaaaagacgatgggttgggaggcgtctaccgaacgcttgtatccgga
agatggtgtgctgaaaggcgacattaaaatggccctgcgcctgaaagatggcggccgctatctggctgacttcaaaacc
acgtacaaagccaagaaacctgtgcagatgcctggcgcgtacaatgtggaccgcaaactggacatcacctctcataatg
aagattatacggtggtagagcaatatgagcgctccgagggtcgtcattctaccggtggcatggatgaactatacaaata
actcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtcctactagtagcggccgct
gcagCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGC
CGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATgaaccttgaccg