##gff-version 3
##sequence-region IBMc175 9 5247
IBMc175	.	CDS	1516	2394	.	-	0	ID=annotation6;Name=araC
IBMc175	.	CDS	3037	3336	.	+	0	ID=annotation7;Name=M86N
IBMc175	.	ribosome_entry_site	2714	2724	.	+	0	ID=annotation10;Name=BBa_B0033
IBMc175	.	primer_binding_site	1371	1390	.	+	0	ID=annotation14;Name=VF2
IBMc175	.	terminator	3789	3841	.	+	0	ID=annotation12;Name=ECK120033736
IBMc175	.	biological_region	1494	1515	.	+	0	ID=annotation11;Name=RFC10 Prefix (full)
IBMc175	.	primer_binding_site	3957	3976	.	-	0	ID=annotation5;Name=VR
IBMc175	.	promoter	9	97	.	+	0	ID=annotation9;Name=tetR promoter
IBMc175	.	promoter	2422	2705	.	+	0	ID=annotation2;Name=PBAD(SapI-)
IBMc175	.	CDS	3502	3777	.	+	0	ID=annotation1;Name=ECF20 102-193
IBMc175	.	biological_region	3842	3862	.	+	0	ID=annotation13;Name=RFC10 Suffix
IBMc175	.	CDS	2731	3033	.	+	0	ID=annotation3;Name=ECF20 1-101
IBMc175	.	CDS	98	1291	.	+	0	ID=annotation4;Name=tetR
IBMc175	.	CDS	3337	3498	.	+	0	ID=annotation8;Name=M86C
IBMc175	.	origin_of_replication	4423	5247	.	+	0	ID=annotation0;Name=p15A origin of replication
>IBMc175
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggc
tggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgat
aggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccct
aactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgc
tgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaat
cgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccg
gcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaa
atattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagc
ctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgt
ccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataa
aaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcag
gggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcaga
cattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaac
aaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaa
ctctctactgtttctccattactagagtcacacaggactactagatgaatgaaaccgatcctgatctggaactgctgaa
acgtattggtaataatgatgcacaggccgttaaagaaatggttacccgtaaactgcctcgtctgctggcactggcaagt
cgcctgctgggtgatgcagatgaagcacgtgatattgcacaagaaagttttctgcgcatttggaaacaggcagcaagct
ggcgtagcgaacaggcacgttttgatacctggctgcatcgtgttgcactgaatctgtgttatgatcgtctgcgtcgtcg
taaagaacatgtgccggttgatagcgaacatGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaa
agagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaat
cagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaa
ggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgct
ctaccccgtaaactagaaagctcctctttacaattggcaccagaaatagaaaagttgcctcagagtgatatttactggg
accccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggactacgtaactttgtcgc
caatgacatcattgtacataacTCAgcctgtgaagcactggatacccgtccggcaccggatgaacagctggaagcaagc
gcacagagccgtcgtatggcacaggcactggatcagctgccggatcgtcagcgtgaagcaattgttctgcagtattatc
aagaactgagcaataccgaagcagcagcactgatgcaaattagcgttgaagccctggaaagcctgctgagccgtgcacg
tcgtaatctgcgtagccatctggccgaagcaccgggtgcagatctgagcggtcgtcgcaaaccgtaatactagagaacg
catgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctactagtagcggccgctgcaggagtcacta
agggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatc
attggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagagatgga
atagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgata
aatctggagccggtgagcgtggatctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagt
tatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaag
cattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct
aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagacccctt
aataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggc
ggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttg
tcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtg
gtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacgggggg
ttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggcca
taacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccaggggaaacgcctggt
atctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcct
atggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgcccc
gttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcct
gtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcag
tgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaagaaggtgttgctgactcataccag
gcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagctttgttgtaggtggaccagttgg
tgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtgatctgatccttcaactcagcaaa
agttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatca
tgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtt