##gff-version 3
##sequence-region IBMc090 183 3600
IBMc090	.	long_terminal_repeat	2606	2627	.	-	0	ID=annotation4;Name=modified R1 for SapI
IBMc090	.	promoter	2472	2501	.	-	0	ID=annotation17;Name=Plac
IBMc090	.	long_terminal_repeat	971	992	.	+	0	ID=annotation0;Name=modified R1 for BbsI
IBMc090	.	long_terminal_repeat	993	1014	.	+	0	ID=annotation12;Name=R2
IBMc090	.	CDS	2131	2427	.	-	0	ID=annotation15;Name=Reporter lacZα
IBMc090	.	biological_region	2624	2624	.	-	0	ID=annotation9;Name=C>A (SapI)
IBMc090	.	terminator	1974	2063	.	+	0	ID=annotation7;Name=ECK120029600
IBMc090	.	biological_region	975	977	.	+	0	ID=annotation18;Name=GCA>TTC (BbsI)
IBMc090	.	biological_region	2630	2632	.	+	0	ID=annotation3;Name=overhang by SapI
IBMc090	.	origin_of_replication	183	771	.	+	0	ID=annotation16;Name=ori
IBMc090	.	biological_region	966	2632	.	+	0	ID=annotation10;Name=BbsI, SapI modified transpsoson
IBMc090	.	CDS	1143	1958	.	+	0	ID=annotation1;Name=KanR
IBMc090	.	biological_region	2626	2627	.	-	0	ID=annotation11;Name=CG>AA (SapI)
IBMc090	.	CDS	2826	3485	.	+	0	ID=annotation2;Name=CmR
IBMc090	.	terminator	3506	3600	.	+	0	ID=annotation19;Name=lambda t0 terminator
IBMc090	.	long_terminal_repeat	2584	2605	.	-	0	ID=annotation8;Name=R2
IBMc090	.	biological_region	968	968	.	+	0	ID=annotation5;Name=A>G (Glycine)
IBMc090	.	biological_region	966	969	.	+	0	ID=annotation6;Name=overhang for BbsI
IBMc090	.	terminator	2072	2128	.	-	0	ID=annotation13;Name=ECK120033737
IBMc090	.	biological_region	973	973	.	+	0	ID=annotation14;Name=G>T (BbsI)
>IBMc090
ccaagcctcgagctgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaagga
tctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccc
cgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccg
ctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcaga
taccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgc
tctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagtta
ccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaac
tgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcgg
cagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgc
cacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcct
ttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccg
tattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcc
tgcataacgcgaaGATCTGGAGCGtCttcCGAAAAACGCGAAAGCGTTTCACGATAAATGCGAAAACTGTCTGGATCTG
AATAATTAATTAAATTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAA
CTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGTTCGAGGCCGCGAT
TAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTA
TCGGTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGAT
GAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATG
CATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATAT
TGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTA
TTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCT
GGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTGTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTT
CTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGA
TACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGGAACGGCTTTTTCAAAAATATG
GTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTACTAGAGtt
gagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagtttt
ttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTA
gccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgcta
ttacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgtt
gtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaattgggtaccgggccccccctcgaggtcct
ccagcttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattg
ttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaa
ctcacattaattgcgttgcgctcactgcccgctttccaccggtTGACCCATAATGTTTTCGCATTTATCGTGAAACGCT
TTCGCGTTTTTCGTGCtCttCTTCAgatctgaaattctgcctcgtgatacgcctatttttataggttaatgtcatgata
ataatggtttcttagacgtcaagtggcactcgagttgatcgggcacgtaagaggttccaactttcaccataatgaaata
agatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactg
gatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtaccta
taaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggccttt
attcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatggg
atagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacga
tttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggttt
attgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggaca
acttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggt
tcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggc
ggggcgtaatttgatatcgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttg
ttcagaacgctcggttgccgccgggcgttttttattggtgagaat