##gff-version 3 ##sequence-region all_or_nothing_Pm 1 3967 all_or_nothing_Pm . terminator 3938 3967 . + 0 ID=T500;Name=T500 all_or_nothing_Pm . promoter 1 1987 . + 0 ID=XylSPm;Name=XylSPm all_or_nothing_Pm . CDS 3126 3854 . + 0 ID=gfp_cassette;Name=gfp_cassette all_or_nothing_Pm . CDS 3135 3848 . + 0 ID=msfGFP;Name=msfGFP;Parent=gfp_cassette all_or_nothing_Pm . sticky_end_restriction_enzyme_cleavage_site 3129 3134 . + 0 ID=NheI;Name=NheI;Parent=gfp_cassette all_or_nothing_Pm . sticky_end_restriction_enzyme_cleavage_site 3849 3854 . + 0 ID=EcoRI;Name=EcoRI;Parent=gfp_cassette all_or_nothing_Pm . engineered_region 3126 3128 . + 0 ID=ATG;Name=ATG;Parent=gfp_cassette all_or_nothing_Pm . CDS 1998 3125 . + 0 ID=LacI_cassette;Name=LacI_cassette all_or_nothing_Pm . engineered_region 3089 3125 . + 0 ID=Coupling;Name=Coupling;Parent=LacI_cassette all_or_nothing_Pm . engineered_region 1998 2008 . + 0 ID=Spacer;Name=Spacer;Parent=LacI_cassette all_or_nothing_Pm . CDS 2009 3088 . + 0 ID=LacI;Name=LacI;Parent=LacI_cassette all_or_nothing_Pm . engineered_region 3855 3937 . + 0 ID=MCS;Name=MCS all_or_nothing_Pm . engineered_region 3855 3913 . + 0 ID=Restriction_sites;Name=Restriction_sites;Parent=MCS all_or_nothing_Pm . primer_binding_site 3914 3937 . + 0 ID=PBS_F24;Name=PBS_F24;Parent=MCS all_or_nothing_Pm . sequence_feature 1988 1997 . + 0 ID=RBS_cassette;Name=RBS_cassette >all_or_nothing_Pm acgttcgtaatcaagccacttcctttttgcattgacgcagggtgtcggaaggcaactcgccgaacgcgctcctatagtt ttcagcgaagcgtcccaaatgtaagaagccgtagtctagggctatctcagttatactacgcacattggcactgggatcg ttcaagcaggcgcggatgctttcgagcttgcggttgcggatgtagttcttcggcgtggtgccggcgtgcttctcgaaca aattgtagagcgagcgtggactcatcatcgccagctccgctaaccgctcaaggctgatattccgtttgagattctcctc aatgaattgaacgactcgctcgaaagacgggttacctttgctgaaaatttcacggctgacattgctgcccagcatttcg agcagcttggaagcgatgatccccgcatagtgctcttggacccgaggcatcgactttgtatgttccgcttcgtcacaaa ctaacccgagtagattgataaagccatcgagttgctggagattgtgtcgcgcggcgaaacggataccctccctcggctt gtgccaattgttgtcactgcacgcccgatcaaggaccactgagggcaatttaacgataaatttctcgcaatcttctgaa taggtcaggtcggcttggtcatccggattgagcagcaatagttcgcccggcgcaaaatagtgctcctggccatggccac gccacaggcaatggcctttgagtattatttgcagatgataacaggtctctaatccaggcgagattaccctcacgctacc gccgtagctgattcgacacagatcgaggcatccgaagattctgtggtgcagcctgcctgccgggcgcccgcccttgggc aggcgaatagagtgcgtaccgacatactggttaacataatcggagactgcatagggctcggcgtggacgaagatctgac ttttctcgttcaataagcaaaaatccatagttcacggttctcttattttaatgtgggctgcttggtgtgatgtagaaag gcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttg caatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccaga agttcgtgctgtcggggcatggggcgacgaattggcggataaaggggatctgctggatattacggcctttttaaagacc gtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgtaattac gtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactga aacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgt tacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtt tcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgcatgggaattagcttga tctgaccaacgaccggtagcggagctatccaacggcggtataccaggaaaacacacagcaggtacatcagaacagtacc atgactgaagaacaaatagttttttcctgatccataaagcagaacggcctgctccatgacaaatctggctccccaacta atgccccatgcagccagcataaccagcataaagtgcagtgtccggtttgatagggataagtccagccttgcaagaagcg gatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaataataatgga gtcatgaccatgcctaggaggaaaaaacatatggtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtct cttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgat ggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacc tccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtgg tggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgg gctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttattt cttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatc tggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctgg ctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggtttt caacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcg caatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacag ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctg caactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgc ccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaag cgggcagcatcatcaccaccatcattaggatggtggtgatgataatggctagccgtaaaggtgaagaactgttcaccgg tgttgttccgatcctggttgaactggatggtgatgttaacggccacaaattctctgttcgtggtgaaggtgaaggtgat gcaaccaacggtaaactgaccctgaaattcatctgcactaccggtaaactgccggttccatggccgactctggtgacta ccctgacctatggtgttcagtgtttttctcgttacccggatcacatgaagcagcatgatttcttcaaatctgcaatgcc ggaaggttatgtacaggagcgcaccatttctttcaaagacgatggcacctacaaaacccgtgcagaggttaaatttgaa ggtgatactctggtgaaccgtattgaactgaaaggcattgatttcaaagaggacggcaacatcctgggccacaaactgg aatataacttcaactcccataacgtttacatcaccgcagacaaacagaagaacggtatcaaagctaacttcaaaattcg ccataacgttgaagacggtagcgtacagctggcggaccactaccagcagaacactccgatcggtgatggtccggttctg ctgccggataaccactacctgtccacccagtctaaactgtccaaagacccgaacgaaaagcgcgaccacatggtgctgc tggagttcgttactgccgcaggtatcacgcacggcatggatgaactctacaaataagaattcgagctcggtacccgggg atcctctagagtcgacctgcaggcatgcaagcttgcggccgcgtcgtgactgggaaaaccctggcgcaaagcccgccga aaggcgggcttttctgt