##gff-version 3
##sequence-region all_or_nothing 1 4276
all_or_nothing	.	sticky_end_restriction_enzyme_cleavage_site	4271	4276	.	+	0	ID=SpeI;Name=SpeI
all_or_nothing	.	sticky_end_restriction_enzyme_cleavage_site	1	8	.	+	0	ID=PacI;Name=PacI
all_or_nothing	.	engineered_region	3976	4270	.	-	0	ID=all_or_nothing_PA1_04s;Name=all_or_nothing_PA1_04s
all_or_nothing	.	promoter	243	295	.	+	0	ID=PA1_04s;Name=PA1_04s;Parent=all_or_nothing_PA1_04s
all_or_nothing	.	CDS	135	242	.	+	0	ID=sRNA_cassette;Name=sRNA_cassette;Parent=all_or_nothing_PA1_04s
all_or_nothing	.	engineered_region	220	242	.	+	0	ID=sRNA_target;Name=sRNA_target;Parent=sRNA_cassette
all_or_nothing	.	engineered_region	135	219	.	+	0	ID=MICc_scaffold;Name=MICc_scaffold;Parent=sRNA_cassette
all_or_nothing	.	terminator	1	134	.	+	0	ID=T1_TE;Name=T1_TE;Parent=all_or_nothing_PA1_04s
all_or_nothing	.	engineered_region	9	3975	.	+	0	ID=all_or_nothing_Pm;Name=all_or_nothing_Pm
all_or_nothing	.	terminator	3946	3975	.	+	0	ID=T500;Name=T500;Parent=all_or_nothing_Pm
all_or_nothing	.	promoter	9	1995	.	+	0	ID=XylSPm;Name=XylSPm;Parent=all_or_nothing_Pm
all_or_nothing	.	CDS	3134	3862	.	+	0	ID=gfp_cassette;Name=gfp_cassette;Parent=all_or_nothing_Pm
all_or_nothing	.	sticky_end_restriction_enzyme_cleavage_site	3137	3142	.	+	0	ID=NheI;Name=NheI;Parent=gfp_cassette
all_or_nothing	.	sticky_end_restriction_enzyme_cleavage_site	3857	3862	.	+	0	ID=EcoRI;Name=EcoRI;Parent=gfp_cassette
all_or_nothing	.	CDS	3143	3856	.	+	0	ID=msfGFP;Name=msfGFP;Parent=gfp_cassette
all_or_nothing	.	engineered_region	3134	3136	.	+	0	ID=ATG;Name=ATG;Parent=gfp_cassette
all_or_nothing	.	sequence_feature	1996	2005	.	+	0	ID=RBS_cassette;Name=RBS_cassette;Parent=all_or_nothing_Pm
all_or_nothing	.	engineered_region	3863	3945	.	+	0	ID=MCS;Name=MCS;Parent=all_or_nothing_Pm
all_or_nothing	.	engineered_region	3863	3921	.	+	0	ID=Restriction_sites;Name=Restriction_sites;Parent=MCS
all_or_nothing	.	primer_binding_site	3922	3945	.	+	0	ID=PBS_F24;Name=PBS_F24;Parent=MCS
all_or_nothing	.	CDS	2006	3133	.	+	0	ID=LacI_cassette;Name=LacI_cassette;Parent=all_or_nothing_Pm
all_or_nothing	.	engineered_region	3097	3133	.	+	0	ID=Coupling;Name=Coupling;Parent=LacI_cassette
all_or_nothing	.	engineered_region	2006	2016	.	+	0	ID=Spacer;Name=Spacer;Parent=LacI_cassette
all_or_nothing	.	CDS	2017	3096	.	+	0	ID=LacI;Name=LacI;Parent=LacI_cassette
>all_or_nothing
ttaattaaacgttcgtaatcaagccacttcctttttgcattgacgcagggtgtcggaaggcaactcgccgaacgcgctc
ctatagttttcagcgaagcgtcccaaatgtaagaagccgtagtctagggctatctcagttatactacgcacattggcac
tgggatcgttcaagcaggcgcggatgctttcgagcttgcggttgcggatgtagttcttcggcgtggtgccggcgtgctt
ctcgaacaaattgtagagcgagcgtggactcatcatcgccagctccgctaaccgctcaaggctgatattccgtttgaga
ttctcctcaatgaattgaacgactcgctcgaaagacgggttacctttgctgaaaatttcacggctgacattgctgccca
gcatttcgagcagcttggaagcgatgatccccgcatagtgctcttggacccgaggcatcgactttgtatgttccgcttc
gtcacaaactaacccgagtagattgataaagccatcgagttgctggagattgtgtcgcgcggcgaaacggataccctcc
ctcggcttgtgccaattgttgtcactgcacgcccgatcaaggaccactgagggcaatttaacgataaatttctcgcaat
cttctgaataggtcaggtcggcttggtcatccggattgagcagcaatagttcgcccggcgcaaaatagtgctcctggcc
atggccacgccacaggcaatggcctttgagtattatttgcagatgataacaggtctctaatccaggcgagattaccctc
acgctaccgccgtagctgattcgacacagatcgaggcatccgaagattctgtggtgcagcctgcctgccgggcgcccgc
ccttgggcaggcgaatagagtgcgtaccgacatactggttaacataatcggagactgcatagggctcggcgtggacgaa
gatctgacttttctcgttcaataagcaaaaatccatagttcacggttctcttattttaatgtgggctgcttggtgtgat
gtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccat
cctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgaga
acaccagaagttcgtgctgtcggggcatggggcgacgaattggcggataaaggggatctgctggatattacggcctttt
taaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatcc
gtaattacgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgag
caaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatg
tggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctg
ggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgcatgggaat
tagcttgatctgaccaacgaccggtagcggagctatccaacggcggtataccaggaaaacacacagcaggtacatcaga
acagtaccatgactgaagaacaaatagttttttcctgatccataaagcagaacggcctgctccatgacaaatctggctc
cccaactaatgccccatgcagccagcataaccagcataaagtgcagtgtccggtttgatagggataagtccagccttgc
aagaagcggatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaata
ataatggagtcatgaccatgcctaggaggaaaaaacatatggtgaaaccagtaacgttatacgatgtcgcagagtatgc
cggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaa
gcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcg
ttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgc
cagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgc
gtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccgg
cgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgt
ggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgt
ctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgt
ccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggc
gctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgatacc
gaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggacc
gcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccac
cctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccga
ctggaaagcgggcagcatcatcaccaccatcattaggatggtggtgatgataatggctagccgtaaaggtgaagaactg
ttcaccggtgttgttccgatcctggttgaactggatggtgatgttaacggccacaaattctctgttcgtggtgaaggtg
aaggtgatgcaaccaacggtaaactgaccctgaaattcatctgcactaccggtaaactgccggttccatggccgactct
ggtgactaccctgacctatggtgttcagtgtttttctcgttacccggatcacatgaagcagcatgatttcttcaaatct
gcaatgccggaaggttatgtacaggagcgcaccatttctttcaaagacgatggcacctacaaaacccgtgcagaggtta
aatttgaaggtgatactctggtgaaccgtattgaactgaaaggcattgatttcaaagaggacggcaacatcctgggcca
caaactggaatataacttcaactcccataacgtttacatcaccgcagacaaacagaagaacggtatcaaagctaacttc
aaaattcgccataacgttgaagacggtagcgtacagctggcggaccactaccagcagaacactccgatcggtgatggtc
cggttctgctgccggataaccactacctgtccacccagtctaaactgtccaaagacccgaacgaaaagcgcgaccacat
ggtgctgctggagttcgttactgccgcaggtatcacgcacggcatggatgaactctacaaataagaattcgagctcggt
acccggggatcctctagagtcgacctgcaggcatgcaagcttgcggccgcgtcgtgactgggaaaaccctggcgcaaag
cccgccgaaaggcgggcttttctgttataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagag
cgttcaccgacaaaaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctcga
gctcgagaaaaaaagcccggacgactgttcgggcttgtctttttatatgttggaaaatcagtggcaatgcaatggccca
acagaaaatggtgaaaccagtaacgttatactgaatctaagtatcaatgtgagcgctcacaagtcaacactctttttga
taaaactagt