A genetic device to digitalize gene expression into a sharp on/off signal.
Digitalizing heterologous gene expression in Gram-negative bacteria with a portable on/off module
Preprint at: https://www.biorxiv.org/content/10.1101/783506v1
AvrII restriction site (cctagg), overlapped with RBS (aggagga)
SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI